optofluidic devices for single cell manipulation ana rita ribeiro, ariel guerreiro, pedro jorge new...
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OPTOFLUIDIC DEVICES FOR SINGLE CELL MANIPULATION
Ana Rita Ribeiro, Ariel Guerreiro, Pedro Jorge
New Challenges in the European Area - Young
Scientist's 1st International Baku Forum
23-05-2013
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Outline
• Introduction
• Main Principles
• Computational Model and Results
• Experimental Preliminary Results
• Conclusions and Future Work
23-05-2013 Young Scientist's 1st International Baku Forum
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What are optical tweezers?
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Motivation• Manipulation: Cell/particle manipulation withouth
physical contact;
• Miniaturization: Diagnostic devices (in vivo cells, ...)
Main Goals• Development of fiber optical tweezers;
• Study of the forces:
• Numerical models;
• Design and optimization of fiber structures;
• Fabrication of devices;
• Field of application: Manipulation of Red Blood Cells.
Liu, Z. et al Opt. Express, OSA, 2006, 14, 12510-12516
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Main Principles
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Ashkin, A., Dziedzic, J. M., Bjorkholm, J.E. and Chu, S. (1986), Opt Lett., 11, 288-290;Ashkin, A. (1970), Phys. Rev. Lett., 24, 156-159.
Particle placed in the focusing region of a laser beam:
• Force transference of momentum on the particle surface (reflections/refractions).
• Scatering Force
• Gradient Force
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Computational model and Results
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Lorentz Force (dipoles):
𝑓 𝑖=14𝜀0(𝜀𝑝−𝜀𝑚)𝛻𝑖|𝐸|2
R. S. RODRIGUES RIBEIRO et al, “Computational Models for New Fiber Optic Tweezers”, Photonic Sensors
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Results
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Preliminary Results
• Lens – polimer:
– radius ~ 30-40 μm;
• Laser Diode 980 nm, P = 100mW;
• Polystyrene particles:
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Experimental Preliminary Results
R. S. Rodrigues Ribeiro, et al., New theoretical and experimental methods for the design of fiber optic tweezers, EWOFS, Poland,2013
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Conclusion and Future Work
• Development of a 2D numerical model to calculate optical forces acting on a particle/cell;
• Fabrication of lenses in optical fibers;
• Implementation of a 3D numerical model;
• Study of distinct fiber geometries;
• Manipulation of RBC.
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Thanks!23-05-2013 Young Scientist's 1st International Baku Forum