one step ahead in allergy management - dr. baddaky€¦ · why is the ige test of choice? 1 three...
TRANSCRIPT
They can’t tell you what they’re allergic to That’s where we come in
Ground-breaking technology
New-generation CCD blockers
3 unique monoclonal antibodies from recombinant IgE
100% specificity with high sensitivity
One step ahead in allergy management
What are CCDs and why is it important to block them?
What is ? Next+ is an innovative allergy test for detecting specific immunoglobulin E (IgE) in the serum of dogs and cats. It has all the benefits of our ground-breaking technology it employs a new generation of CCD blockers.
Specific allergen protein Carbohydrate chains
CCDs are cross-reactive carbohydrate determinants – the carbohydrate chains found in glycoproteins. CCDs are part of the structure of many allergy-causing proteins, especially plant-based allergens like pollen. During an allergic reaction, IgE is produced against the carbohydrate chains as well as the allergen proteins. Studies have confirmed that this occurs in
30% of humans, dogs and cats [1-4].Other research shows that IgE against CCDs is clinically irrelevant. But it is now known that removing CCD-specific IgE makes in vitro test correlate well with the sensitivity and specificity of intradermal skin tests [4]. Blocking CCDs simply means that the specificity of the in vitro test is enhanced.
STEP 1 – CCD SCREENING STEP 2 – CCD BLOCKING
CCD-specific IgE Specific allergen protein CCD chain CCD-blockerAllergen-specific IgE
slgE
Relevant positive
SAMPLE 3 SAMPLE 3
Irrelevant positive
SAMPLE 4 SAMPLE 4
slgE
Relevant positive
SAMPLE 2
slgE
Relevant positive
slgE
Relevant negative
slgE
Serum
Relevant negative
SAMPLE 1
slgE
Why is the IgE test of choice?1 Three proprietary monoclonal antibodies
derived from recombinant IgE• Unique monoclonal antibodies that target
specific IgE sites• Developed from canine recombinant IgE• No cross-reactions with other
immunoglobulins like IgG• 100% specificity and superior sensitivity • Fewer in-lab steps to minimize the risk
of inaccuracy
2 New-generation semi-synthetic CCD blockers
• First test with next-generation CCD blockers • Highly specific blocking of CCDs• No interference with any tested allergens
3 Excellent accuracy and reproducibility• Best-in-class intra-assay and inter-assay
reproducibility• Daily calibration curve removes variability
IgE, IgG and BSA as antigens in differents wells
IgE
O.D
.
3,5
3
2,5
2
1,5
1
0,5
0 0,05 0,10 0,20 0,39 0,78 1,56 3,13 6,25 12,50
IgG BSA
Protein Amount [Nanogram / Well]N
orm
aliz
ed A
.U.
OD IgE curve
0 0,2 0,4 0,6 0,8 1 1,2 1,4
y = 1,1571x - 0,0116R2 = 0,9996
1,4
1,2
1
0,8
0,6
0,4
0,2
0
ALLERGENS WITHOUT CCD BLOCKER
WITH CCD BLOCKER
Phleum pratense 486 375
Poa pratensis 205 52
Dactylis glomerata 259 32
Lolium perenne 181 81
Rumex acetosella 352 52
Plantago lanceolata 316 66
Urtica spp. 205 50
Chenopodium album 358 108
Artemisia vulgaris 235 35
Ambrosia eliator 409 49
Betula pendula 428 415
Corylus avellana 247 47
Salix viminalis 100 60
Ulmus americana 121 21
Positive allergens 7 2
[1] Petersen A et al Ubiquitous structures responsible for IgE cross-reactivity between tomato fruit and grass pollen allergens J Allergy Clin Immunol 1996 Oct; 98(4):805-15
[2] Mari A et al Specific IgE to cross-reactive carbohydrate determinants strongly affect the in vitro diagnosis of allergic diseases J Allergy Clin Immunol 1999; 103(6): 1006-1011
[3] Gedon NKY et al Agreement of serum allergen test results with unblocked and blocked IgE against cross-reactive carbohydrate determinants (CCD) and intradermal test results in atopic dogs Vet Dermatol 2019; 30(3):195
[4] Álvarez J Unpublished data Alergovet S.L.
ArtuvetVijzelweg 11, 8243 PM [email protected]+31 320 783 100artuvet.com
Alergovet Valentin Beato 24, 28037 [email protected]+34 914 134 472alergovet.com
Dr BaddakySnickarvägen 7, 673 32 [email protected]+46 (0)571-202 30drbaddaky.com
NEX
T9I1
9
nextmune.com
One step ahead in allergy management