olympus ck40-rfa reflected light fluorescence attachment ... · ck40-rfa d. repair procedure 5....

REFLECTED LIGHT

FLUORESCENCE ATTACHMENT

CK40-RFA

REPAIR MANUAL

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OLYrvjPUS

All rights reserved. Reproduction in whole or in part without written permission is prohibited. (8H) f

CK40-RFA

cm‘ CONTENTS

A. OUTLINE OF PRODUCT 1. Outline . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-l 2. Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-l 3. Using conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-l 4. Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-2 5. Dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-3

B. INSPECTION STANDARD

f 1. Inspection items and methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-l

C. DISASSEMBLY AND ASSEMBLY PROCEDURES 1. Slider unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-l 2. Cube dovetail/diaphragm unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . c-3

D. REPAIR PROCEDURE 1. Adjustment procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . D-l 2. Adjusting the cube working force . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . D-2 3. Adjusting the click removing force of the cube . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . D-2 4. Adjusting the click removing force of the slider . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . D-3 5. Adjusting the optical axis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . D-4 6. Checking the FS image center . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . D-7

r E. JIGS AND TOOLS

1. List of jigs and tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . E-l

F. LUBRICANTS AND CHEMICALS 1. List of lubricants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . F-l 2. List of chemicals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . F-l

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CK40-RFA A. OUTLINE OF PRODUCT

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2.

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3.

P

Outline

(1) This product is an illuminator of a reflected fluorescence system designed for CK40.

(2) Target market and users Target market: Tissue culture routine market represented by culture, growth, immunity and pharmacy

(3) Applicable microscope: CK40

Features

(1) Switching between B excitation and G excitation is easy because the 2-cube IN-OUT method is adopted.

(2) Two-wavelength observation and transmitted light observation can be set simultaneously.

Using conditions

(1) This illuminator is designed for B excitation and G excitation observation only. (This is

(2) because the lamp housing (U-LHSOHG) has a built-in ultraviolet protective filter.) Use a shutter for transmitted light observation.

(3) Be sure to mount the shield covers to the RFA base from both sides of cube. This prevents flashlight.

(4) Weak flashlight goes into the visual field at the time of cube switching. However, this

(5) involves no hazard because the lamp housing has a built-in ultraviolet protective filter. The standard performance is not guaranteed if it is combined with a lamp housing other than U-LHSOHG.

(6) The standard performance is not guaranteed if an objective other than the designated types is used. (Refer to Item 11 in “4. Specifications.“)

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CK40-RFA A. OUTLINE OF PRODUCT

4. Specifications

Item Specitications Remarks

1 Weight 1.14 kg CK40-RFA single unit

2 Field number 3 Projection

magnification 4 Diaphragm

5 Observation method

F.N. 20 3.139x

Built-in field diaphragm (with centering mechanism) $2-$5mm

1) Reflected light fluorescence (B excitation, G The lamp housing excitation) (U-LHSOHG) has a

2) Transmitted light observation built-in ultraviolet protective filter (L-42).

6 Observation sxtitching 1) Movable dovetail type (with optical path click) method 2) Maximum of 2 cubes

7 Shutter Slide type (with a click at the center and stoppers on the both ends)

8 Filter With a filter pocket 32ND25

9 Reflected light 1) Supported excitation: B excitation, G excitation fluorescence 2) Cube type: Built-in dichroic mirror, excitation

filter and barrier filter

10 Cube

3) Auxiliary excitation filter: None 4) Auxiliary barrier filter: None 1) Cube type

CX-DMB-2 for B excitation CX-DMG-2 for G excitation

2) The cube for fluorescence has built-in fixed dichroic mirror, excitation filter, and barrier filter.

11 Objective SPlan4XPL-6 DAchlOXPL-6 LWDCDAch20XPL-6 LWDCDPlan40X

Designated objective

A-2

A. OUTLINE OF PRODUCT CK40-RFA

r‘ 5. Dimensions

Unit: mm

A-3

CK40-RFA B. INSPECTION STANDARD

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1. Inspection items and methods

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Part Item Standard Method

lluminator Cube working 250 t 50 g Insert the cube with no click pressure applied to it force (2450 mN 2 490 n-8) and measure the working force using a tension gauge.

Click removing 750 + 50 g Insert the cube with click pressure applied to it and force of cube (7350 InN t 490 mN) measure the click removing force using a tension

gauge.

Click removing 500 + 100 g Insert the slider and measure the click removing force force of slider (4900 mN 2 980 InN) using a tension gauge.

Optical axis 0.3 mm or less Combining the standard eyepiece (KNO048; with adapter-l), a microscope stand (product), the standard objective (KNOO15), and the cube (BH2- UBF), read the displacement between the specimen center and the visual field center in the right sleeve using the reticle scale (1 graduation=O.l mm) of the KNO048 (with adapter-l).

FS image It can be set at the center Combining the NCWHKlOX, a microscope stand center of the visual field. (product), the cube (product: CX-DMB-2, CX-

DMG-2), U-LHSOHG (product), and U-RFLTSO (product), focus on a name card (coloured with a fluorescent pen) used as a specimen and make sure that the FS image center can be set at the center of the visual field.

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CK40-RFA C. DISASSEMBLY AND ASSEMBLY PROCEDURES

1. Slider unit

0

-Q

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pply adhesive to the screw

c-2


2. Cube dovetail/diaphragm unit

:-q!/i @a=$&& xiJ

*1

c-3


No. Parts name

@ COVER

Screw Grease Adhesive Remarks

0 SCREW OT1131 Apply adhesive to the screw. Refer to “3. Adjusting the click removing force of the cube (D-2).”

0 SPRING B3UO (*l)

@ SCREW (4 PCS.) OT1131 Apply adhesive to the screws. Refer to “2. Adjusting the cube working force (D-2).”

@ SPRING (4 PCS.)

@ COVER PSK2X3SA (*2) 6 PCS.

0 DOVETAIL (2 PCS.)

@ PLATE (2 PCS.)

@ DOVETAIL AB3X12SA (*3) 2 PCS. OT1595 OT1131 Apply adhesive to the screws (*3). Apply grease to the dovetail surface only. Refer to “5. Adjusting the optical axis @-4).”

@ BASE

@ FIXING SPRING OT2008 Apply grease to the reverse side.

@ LEVER-l OT1983 Apply adhesive to the screw thread.

@ SPRING HOLDER OT1983 Apply adhesive to the screw thread.

8 SPRING

@ SPINDLE

8 KNOB (2 PCS.) OT2008 Apply adhesive to the screw thread.

@ FSFRAME ANU3X4SA (*4) 3 PCS. OT1131 Apply adhesive to the screws (*4). Refer to “6. Checking the FS image center (D-7).”

@ RING

@ INNERFRAME

8 DIAPHRAGM BLADE-A (8 PCS.)

@ OUTERFRAME

@ SPRING AHU4X4SA (*5) Refer to “4. Adjusting the click B3UO (*6) removing force of the slider (D-3).”

@ SLIDER HOLDER-2 ABSX12SA (*7) 2 PCS.

c-4

CK40-RFA D. REPAIR PROCEDURE

1. Adjustment procedure

2. Adjusting the cube working force

(Refer to D-2.)

3. Adjusting the click removing force of the cube

(Refer to D-2.)

1

4. Adjusting the click removing force

Of the ‘lider I (Refer to D-3.) 1

5. Adjusting the optical axis (Refer to D-4.)

6. Checking the FS image center (Refer to D-7.)

D-l


2. Adjusting the cube working force

2-1 Set the cube shown in the following illustration to adjust the working force using a tension gauge.

Insert a set of SPRING @ and SCREW 0 at four locations, fit the CX-DMB-2 0 (product) on the DOVETAIL 0, and measure the working force in the whole stroke using a TENSION GAUGE 0.

Setting the end of the tension gauge on the (B, G) knob position, measure the working force.

Standard working force: 250 2 50 g (2450

If the result is not within the standard range, adjust it by tightening or loosening the four pieces of SCREW 0.

3. Adjusting the click removing force of the cube

3-1 Set the cube shown in the following illustration to adjust the click removing force using a tension gauge.

Insert a set of BALL @, SPRING 0 and SCREW @>, fit the CX-DMB-2 0 (product) on the DOVETAIL 0, move it to the clicking position, and measure the click removing force using a TENSION GAUGE 0.

Setting the end of the tension gauge on the (B, G) knob position, measure the removing force.

Standard removing force: 750 -c 50 g (7350 mN 2 490 mN)

If the result is not within the standard range, adjust it by tightening or loosening the SCREW 63,.

D-2


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4. Adjusting the click removing force of the slider

4-1 Set the slider shown in the following illustration to adjust the click removing force using a tension gauge.

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f-

Slider @

gauge 0

Insert a set of BALL @, SPRING 0 and SCREW @, move the SLIDER 0 to the clicking position of the SLIDER HOLDER-l 0, and measure the click removing force using a TENSION GAUGE 0.

Setting the end of the tension gauge at the center of the knob, measure the removing force.

(“ Standard removing force: 500 + 100 g (4900 mN + 980 mN)

If the result is not within the standard range, adjust it by tightening or loosening the SCREW 63.

D-3


5. Adjusting the optical axis

5-l Perform the adjustment in the following procedure.

(1) Remove the AB3X20SA screws (“1) from the LH MOUNT 0 of CKLCO-RFA 0. Instead of the LH MOUNT 0, mount the BXKC002 (optical alignment jig) and RFA unit on CK40 MAIN BODY 0 with AB3X25SA.

(2) Insert the CUBE (CX-DMB-2) 0 into the dovetail of CK40-RFA 0 and mount the COVER ASS’Y L/R 0 (The cube should be inserted in such a way that the characters on the CX-DMB-2 are seen in the reverse).

/ a

D-4


(3) Mount the KNOB ASS’Y-B on the cube through the COVER-R and move the cube to the optical path.

5-2 Setting the jigs shown below, adjust the optical axis.

Centering telescop (KN0029)

Optical alignment jig for illumination part (BXKC002)

Standard objective for optical axis (KN0015)

5-3 Turn on the power of CK40 main body and observe the optical axis position using the centering telescope.

D-5


5-4 Loosen two SCREWS 0 and move the CUBE DOVETAIL 0 to adjust the optical axis.

Standard: Within 3 graduations on reticle scale of centering telescope (KNOO29)

If the result is not within the standard range, return to “Adjusting the optical axis” on page D- 4 and repeat the work.

D-6


6. Checking the FS image center

6-1 Set the following jig and products to check the FS image center:

l Eyepiece . . . . . . . . . . . . NCWHKlOX

l Cube . . . . . . . . . . . . . . . . . . CX-DMB-2 or CX-DMG-2 (product)

l Standard tube . . . . BKN0003 (Use it when no observation tube is available.)

l Reflected light fluorescence unit . . . . . . . . . . . . . U-LHSOHG, U-RFLTSO (product)

l Other . . . . . . . . . . . . . . . . . Name card (coloured with a fluorescent pen)

D-7


( 6-2 Mount the CK40-RFA on the CK40 main body, turn off the power of main body, set the

microscope to reflected fluorescence observation, and move the two KNOBS 0 to make sure that the FS image center can be set at the center of the visual field.

D-8

CK40-RFA E. JIGS AND TOOLS

1. List of jigs and tools

No.

CX-DMB-2 (product) Cube

Description Page

D-2 - 8 or CX-DMG-2 (product)

OT1144 OT1145 KNO029

BXKC002 KNOOlS

U-LHSOHG (product)

Tension gauge (500 g)

Optical alignment jig for illumination part

Tension gauge (1 kg) Centering telescope

D-2 D-2 - 3 D-4 - 6

1 D-4 - 6 LB standard objective for optical axis D-4 - 6 Lamp housing D-7 - 8 a

I U-RFLT.50 (product) Power supply unit 1 D-7 - 8

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CK40-RFA F. LUBRICANTS AND CHEMICALS

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1. List of lubricants

<Grease>

No. Description

OT1595 Silicone grease OT2008 Grease (medium)

c-2, 4 c-4

Page

2. List of chemicals

p <Adhesive>

No.

OT1131 OT1983

Description

Shellac Solvent-based adhesive (red)

c-2, 4 c-4

Page

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olympus ck40-rfa reflected light fluorescence attachment ... · ck40-rfa d. repair procedure 5....

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