obtain more information from your target · more complete biological assessment. in vivo....

In Vivo Imaging Agents

OBTAIN MOREINFORMATIONFROM YOURTARGET

For research use only. Not for use in diagnostic procedures.

2www.perkinelmer.com/invivoreagents2

TARGETEDTO YOUR APPLICATION AND

VALIDATEDFOR YOUR CONFIDENCE

MMP and cathepsin activity in 4T1 tumors by MMPSense® 680 and ProSense® 750 EX

Bioware Brite Red-Fluc transduced A549 lung cancer cells

Cathepsin activity in antibody induced Arthritis by ProSense 750 EX

Migration of UTI infection from bladder to kidney by Proteus mirabilis strain Xen44

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Activatable Fluorescent Agents

Activatable agents are optically silent upon injection but are activated in vivo through cleavage by specific protease biomarkers of disease. Benefits include biologically specific readouts and high signal-to-noise at the target biology. The FAST platform represents the next generation of agents from PerkinElmer. Utilizing a novel small molecule design, the FAST agents offer improved specificity, accelerated activation profile, and earlier imaging timepoints.

PerkinElmer’s comprehensive suite of fluorescent in vivo imaging agents enables unmatched imaging of a broad range of

disease-related biomarkers and pathways in your research models. Our fluorescent agents and labels are optimized for use in the full range of PerkinElmer optical in vivo imaging systems, as well as other fluorescence microscopy systems and many in vitro and cell-based systems.

MORE INSIGHTFULRESEARCHRESULTS

VivoTag

Linker

Fluorescentdyes quenched

by proximity

VivoTag

LinkerProtease-specific peptide

VivoTag

Linker

Peptide cleaved by protease

VivoTag

Linker

Dye fluoresces

Dye fluoresces

Target protease

Activatable agents mechanism of action.

Fluorescent images of Neutrophil Elastase 680 FAST activity in control and arthritic mice using the IVIS Spectrum.

FLUORESCENT IN VIVO IMAGING AGENTS

Cat B 680 FAST Selective imaging of cathepsin B proteinases (Cat B). Optically silent in the inactivated state, NEV11112 becominghighlyfluorescentwhenactivated. Cat B 750 FAST NEV11098

CatK680FAST ImagingofcathepsinKactivityinoncologyapplicationsinvolvingmetastasistotheboneaswellasabroad NEV11000 rangeofboneapplicationsincludingboneloss,tumor-inducedosteolysisandbonechangesfollowingarthritis.

MMPSense 645 FAST NEV10100 MMPSense 680 NEV10126

MMPSense 750 FAST NEV10168

Neutrophil Elastase 680 FAST Fluorescent neutrophil elastase-activatable agent that is optically silent upon injection and produces NEV11169 fluorescentsignalaftercleavagebyelastaseproducedbyneutrophilcells.

ProSense 680 NEV10003 ProSense 750 EX NEV10001EX

ProSense750FAST FASTversionofProSense,withfasterkineticsandabroaderimagingwindow. NEV11171

ReninSense680FAST Imagingofrenin-angiotensinpathwayassociatedwithhypertension,kidneyandcardiovasculardisease. NEV11079

Product Product Description Catalog Number

Imaging of MMP (metalloproteinase) activity is involved in many disease-related phenomena including cancer propagation, invasion and metastasis, rheumatoid arthritis and areas of cardiovascular disease.

Versatile imaging of changes in cathepsin-based protease activity as seen in a number of pathological states and disease-related events including rheumatoid arthritis, cancer, atherosclerosis, angiogenesis and cardiovascular disease.

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Targeted Fluorescent Agents

Optimized in vivo imaging agents that actively target and bind to specific biomarkers. Benefits include the agents’ highly specific targeting to key biological mechanisms.

BacteriSense mechanism of action

Positively-charged small molecules

labeled with fluorescent VivoTag

Bind to negatively-charged membrane lipids

VivoTag

+ + +

VivoTag

+ + +


Optimized imaging of bone turnover through binding of hydroxyapatite.

Annexin-Vivo 750 In vivo targeting of membrane-bound phosphatidylserine exposed during the early stages of apoptosis. NEV11053

BacteriSense645 Fast-clearing,targetedprobewhichbindstonegativelychargedlipidsonthebacterialcellmembrane, NEV10080 enabling the monitoring of infection progression in real time.

BombesinRSense 680 Target and quantify up-regulation of bombesin receptors (BBR) in vivoassociatedwithtumorproliferation. NEV10090 These receptors are also over-expressed in a variety of cancers.

FolateRSense680 HighlyspecificandsensitiveindetectionofFolateReceptorprotein.Canbeusedtocloselymonitorand NEV10040 quantitatetumorgrowthandmetabolism.

HypoxiSense680 Detectsthetumorcellsurfaceexpressionofcarbonicanhydrase9(CAIX)protein,whichincreasesin NEV11070 hypoxicregionswithinmanytumors.

IntegriSense 645 NEV10640



OsteoSense 680 EX NEV10020EX

OsteoSense 750 EX NEV10053EX

OsteoSense 800 NEV11105

TLectinSense680 NIR-labeledTomatoLectinproteinwhichhashighbindingaffinityforglycoproteinN-acetylglucosaminesonthe NEV10060 surface of vascular endothelial cells. Use for vascular mapping in vivo.

Transferrin-Vivo750 NIR-labeledtransferrindetectstransferrinreceptorupregulationassociatedwiththeincreasedcellmetabolic NEV10091 needforironincancerandinflammatorycells.Alsodetectsnormalironmetabolismintheliver.

Targets integrin avβ3expressedinoncolo≠gy,atherosclerosisandangiogenesisdiseasemodels.

Multimodal Detection with Bioluminescent and Fluorescent Imaging Agents in the Same Animal Reveals the Context of Disease

In vivo fluorescent imaging of same Bioware Brite U-87 MG-Red-Fluc orthotopic tumor mouse. Mouse was injected with a single dose of IntegriSense750 imaging agent to detect expression of integrin avb3 and imaged using the IVIS SpectrumCT instrument 24 hrs post-injection. Three-dimensional FLIT (Fluorescent Imaging Tomography) reconstruction of the signal shows precise localization of the avb3 expressing tumor.

UsingfluorescentandbioluminescentimagingagentsinconjunctionwithmicroCTandopticalimaginginstrumentationprovidessynchronizationoffunctional and anatomical data, simultaneously and co-registered, for true quantitative 3D image data. Composite functional and anatomical imaging obtained by using fluorescent and bioluminescent agents together gives a clearer context and understanding of the mechanisms of disease. Imaging thereagentcombinationwiththePerkinElmer'sIVIS® SpectrumCT and Quantum enables the co-registration of microCT and optical image data for more complete biological assessment.

In vivo bioluminescent imaging of Bioware Brite U-87 MG-Red-Fluc orthotopic tumor mouse. In this study, 300,000 cells were implanted directly into the brain of nude mice and tumors were imaged using the IVIS Spectrum optical system two weeks post-injection. Three-dimensional DLIT (Diffuse Light Imaging Tomography) reconstruction of bioluminescent signal shows precise location of the brain tumor.

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TVascular and Physiological Fluorescent Agents

PerkinElmer’s vascular and physiological agents are a range of highly fluorescent in vivo imaging molecules that remain highly stable and localized in the anatomy for various periods of time to enable imaging of disease physiology, vasculature, vascular permeability and angiogenesis.

AngioSense 680 EX NEV10054EX AngioSense 750 EX NEV10011EX

AngioSPARK 680 NEV10149

AngioSPARK 750 NEV10150

GastroSense 750 Imaging to monitor gastric emptying and the impact of various drugs on gastric motility; may also NEV11121 beusedasananatomicalmarkerforthestomach.

Genhance 680 (1 mg) NEV10117




GFR-Vivo680 NIRfluorescentimagingagenttonon-invasivelydetermineglomerularfiltrationrate(GFR)in vivo in models NEV30000 ofkidneydisease,dysfunction,anddrugtoxicity.

Superhance680 Imagingofvascularity,perfusionandvascularpermeability;shortpharmacokineticprofile. NEV10116


Imagingofvascularity,perfusionandvascularpermeability;longpharmacokineticprofile.

Imaging of vascularity, perfusion and vascular permeability. Remains localized in vasculature for 0-4 h; accumulates in tumors and arthritic joints at 24 h.

Smallmoleculefluorescenceagent.Useasacontrolorinvascularpermeabilityimaging.

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Measure Kidney Function Non-Invasively in vivo

Glomerularfiltrationrate(GFR)isthegoldstandardinkidneyfunctionassessmentandisusedtodetermineprogressionofkidneydiseaseanddrug-inducedkidneytoxicity.GFR-Vivo™ 680 is a near infrared (NIR) fluorescent-labeled form of inulin in a spectral regionprovidinglowbackgroundandhightissuepenetration(ex/em=670/685nm)forin vivo application.

Fluorescencemoleculartomographic(FMT)imagingoftheheartwasusedtodetectandquantify blood levels of GFR-Vivo 680 at multiple time points, providing the necessary datatocalculatetheclearanceratesinindividualanimals.Followinganintravenousbolus of GFR-Vivo 680 in SKH-1E mice, FMT®imageswereacquiredat1,5,15,30,and45minutespost-injectionGFR-Vivo680,incombinationwithFMTheartimaging,provides a non-invasive fluorescent imaging approach to generate consistent GFR measurementsinmodelsofkidneydisease,dysfunction,anddrugtoxicity.


XenoLight® RediJect™ COX-2 133316 ProbeExplorerkit(5injections)

XenoLight® RediJect™ 133314 COX-2ProbeStandardkit (20 injections)

XenoLight® RediJect™ Non reactive control dye for COX-2 probe 133349 COX-2 Probe Control dye (5 injections)

XenoLight® RediJect™ 133397

Bacterial Detection Probe 750 (5 injections)


Bacterial Detection Probe 750 (20 injections)

XenoLight® RediJect™ Non reactive control dye for RediJect Bacterial Detection Probe 133399

Bacterial Detection Probe Control dye (5 injections)


2-DG750ProbeExplorerkit (5 injections)


2-DG750ProbeStandardkit (20 injections)

XenoLight® RediJect™ 2-DG Non-reactive control dye for RediJect 2-DG 750 probe 760567

750 control dye (5 injections)

NIR targeted probe for non-invasive detection of bacterial infections in vivo

Imagingprobethatspecificallydetectsthecyclooxygenase-2(COX-2)

NIRtargetedprobefornon-invasiveimagingofglucoseuptakein vivo

Targeted Fluorescent XenoLight Probes


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Fluorescent Labels and Dyes

PerkinElmer fluorochromes and nanoparticles are designed specifically to enable customized development of novel superbright fluorescent imaging agents, with properties that are ideal for use in in vivo or in vitro imaging.

AminoSPARK 680 (3 mg) NEV10142 AminoSPARK 750 (3 mg) NEV10143

VivoTag-S 680 (1 mg) NEV10121




VivoTag-S 750-MAL (1 mg) NEV11223 VivoTag-S 750-MAL (5 mg) NEV11224

VivoTag 645 (1 mg) NEV11173 VivoTag 645 (5 mg) NEV11174

VivoTag 645-MAL (1 mg) NEV11273

VivoTag 645-MAL (5 mg) NEV11274

VivoTag 680 XL-MAL (1 mg) NEV11219

VivoTag 680 XL-MAL (5 mg) NEV11220

VivoTag 680 XL (1 mg) NEV11119 VivoTag 680 XL (5 mg) NEV11120

VivoTag680XLProtein Aneasyandconvenientwaytolabelupto10mgofprotein.Eachkitcontainsoursuperiorin vivo optimized NEV11118 Labeling Kit VivoTag 680XL (2 x 250 μg) and everything you need to carry out the reaction and purify the labeled protein.

VivoTag800(1mg) Smallmoleculefluorochrometolabelatargetligand.Optimizedforhigh-densityloading. NEV11107

VivoTag800(5mg) Smallmoleculefluorochrometolabelatargetligand.Optimizedforhigh-densityloading. NEV11108

VivoTrack680Explorer NIRwatersolublecelllabelingagentthatcangeneratebrightly-labeledandhighlyviablecellssuitablefor NEV12001 detectionandlongitudinaltrackingin vivo. Contains 1 vial that can stain up to 2 x 108 cells.

VivoTrack680Standard NIRwatersolublecelllabelingagentthatcangeneratebrightly-labeledandhighlyviablecellssuitablefor NEV12000 detectionandlongitudinaltrackingin vivo. Contains 5 vials, each vial can stain up to 2 x 108 cells.

XenoLight® DiR (25 mg) NIR dye for non-invasive imaging of cell homing (stem cells, T cells) 125964


Smallmoleculefluorochrometolabelatargetligand.Optimizedforsinglemoleculeloading.Amine-reactiveforlabelingviaanNHSesterlinkage.

Nanoparticlefluorescentlabelforatargetligand.Superbrightwithextendedpharmacokineticprofileandtheabilityformultivalentligandcoupling.

Amine-reactivenearinfraredfluorochromeforlabelingviaanNHSesterlinkagetopeptides,smallmolecules,proteins, antibodies or macromolecules. Optimized for in vitro-in vivo imaging.

Redfluorochromeforcouplingviamaleimidechemistrytolabelfreecysteinesorthiolgroups.Optimized for in vitro-in vivo imaging.

NIRfluorochromeforcouplingviamaleimidechemistrytolabelfreecysteinesorthiolgroups.LowerquenchingthanVivoTag-S680.

Fluorochrome for labeling small molecules, proteins, antibodies, nanoparticles or other macromolecules.Hydrolyticallystable.Lowself-quenchingforhigherloading.

Smallmoleculefluorochrometolabelatargetligand.Optimizedforsinglemoleculeloading.Thiol-reactive for coupling via maleimide chemistry to label free cysteines or thiol groups.

Fluorescent images using the IVIS Spectrum of VivoTrack™ 680-induced macrophages trafficking to carrageenan-induced inflamed paw (right) but not control pay (left).

Thioglycollate-elicited peritoneal macrophages labeled with VivoTrack 680 (bottom) or unlabeled (top).

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Fluorescent Panel Part Number Used To Panel Contents

Arthritis Panel NEV20003Detect molecular players in arthritis pathology

AngioSense 750EXCAT B 750 FAST

CAT K 680 FASTMMPSense 680

OsteoSense® 680EXProSense 680

Tumor Metabolism Panel NEV20015

Non-invasively measure tumor microenvironment biology for early detection of treatment-induced biological changes

AngioSense 680EXAnnexin-Vivo™ 750BombesinRSense™

680

FolateRSense 680MMPSense 750 FAST

ProSense 680Transferrin-Vivo™ 750

Toxicology Panel NEV20016

Detect molecular players and measure early changes in drug-induced injury

AngioSense 680EXAnnexin-Vivo 750

MMPSense 750 FAST*ReninSense™ 680

FASTTransferrin-Vivo 750

Oncology Panel NEV20005

Non-invasively characterize the tumor environment to assess biological changes associatedwithdiseaseprogression or treatment efficacy

AngioSense 680EXFolateRSense™ 680

IntegriSense 750 MMPSense 750 FAST

ProSense 750 EX

InflammationPanel NEV20013

Quantifyinflammationbiolo-gy, particularly in longitudinal studies for understanding disease pathogenesis

AngioSense 680EXFolateRSense 680

IntegriSense 750MMPSense 750 FAST

Neutrophil Elastase 680 FAST

ProSense 750 FAST

Vascular Panel NEV20014Characterize biological changesassociatedwithvascular injury and disease

AngioSense 680EXCAT B 680 FAST


ProSense 750 FAST

FeaturedProbesPack NEV20011

Approach different research areas by sampling our most popular and published fluorescentprobes

AngioSense 680EXFolateRSense 680


ProSense 680VivoTag™ 680XL Protein

Labeling Kit

SamplePack NEV20000

Combinefluorescenceandbioluminescent imaging by sampling our most popular andpublishedfluorescentprobes,aswellasready-to-use injectable luciferin for bioluminescent imaging

AngioSense 680EXIntegriSense™ 750

MMPSense 750 FASTProSense 750 EX

XenoLight® RediJect™ D-Luciferin

With PerkinElmer’s Fluorescent In Vivo Imaging Panels, getting the results you need has never been easier. Our most published fluorescent imaging agents are bundled into application panels targeting key disease areas to enhance your imaging data. Each application panel contains up to seven fluorescent probes, carefully grouped together and designed to optimize your findings and simplify your research.

FLUORESCENT IMAGING PANELS

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*2MMPSenseareprovidedfortheAnnexin/MMPSense/TransferrincocktailasdetailedintheFluorescentPanelUserGuide.

Allpanelscontainfullsizefluorescentprobes(10doses)exceptfortheSamplePackwhichcontainssamplesizes(3doses)ofeachfluorescentprobeand1fullsizeXenoLightRediJectD-luciferin(50doses).

Panels are not open to substitution.


Built around your applications – choose one, or use in combination for your disease focus to obtain more information (see list)

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Easily activated fluorescence probes enable specific imaging of biological processes that underlie disease

Cat B 680 FAST � � � � � � � �Cat B 750 FAST � � � � � � � �Cat K 680 FAST � � � � �MMPSense® 680 � � � � � � � � �MMPSense 645 FAST � � � � � � �MMPSense 750 FAST � � � � � � � � � � � �Neutrophil Elastase 680 FAST � � � � �ProSense® 680 � � � � � � � � �ProSense 750EX � � � � � � � �ProSense 750 FAST � � � � � � � � �ReninSense™ 680 FAST � � �Targeted probes enable specific areas of interest to be detected, monitored and measured in vivo

2-DG 750 Probe �Annexin-Vivo 750 � � � � �BacteriSense™ 645 �Bacterial Detection Probe 750 �COX-2 Probe � �FolateRSense™ 680 � � � � � � � �IntegriSense™ 645 � � � � � � �IntegriSense 680 �IntegriSense 750 � � � � � � � � �HypoxiSense 680 �Inflammation Probe � � �OsteoSense® 680EX � � � � �OsteoSense 750EX � � � �OsteoSense 800 � � � �TLectinSense™ 680 � � �BombesinRSense™ 680 � �Transferrin-Vivo™ 750 � � �Vascular and physiologic fluorescence agents are distributed passively through blood vessels to enable imaging of vascularity, blood pooling near tumors and edema

AngioSense® 680EX � � � � � � � � � � �AngioSense 750EX � � � � � � �AngioSPARK 680 � � � � � �AngioSPARK 750 � � � � � �Superhance 680 � � � � �GFR-Vivo 680 �Optical Reporter Oncology Cell Lines and Microorganisms

Bioware® Brite Cell Lines � � �Bioware Microorganisms �RediFect Lentiviral particles � � �

FLUORESCENT AGENTS BY DISEASE AREA

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Expand your oncology models to deep tissue tumors with brighter, red-shifted cell lines. PerkinElmer’s new Bioware® Brite light-producing cell lines are significantly brighter than other firefly luciferases. In vitro studies have shown that Red-Fluc is 10-20 fold brighter*. Available in a wide range of cancer models including breast, colorectal, lung, and prostate, the cells have been stably transduced with the red-shifted firefly luciferase gene from Luciola Italica (Red-Fluc), for a brighter, red-shifted signal.

BIOWARE® BRITE BIOLUMINESCENT ONCOLOGY CELL LINESBy emitting intensified, longer wavelength light, our bioluminescent oncology cell lines allow you to visualize and monitor the growth of deep tissue tumors in vivo.

The optimized Red-Fluc luciferase enables more sensitive in vivo optical imaging with less tissue attenuation so you can detect tumor development earlier, and monitor tumor growth and metastases in both subcutaneous and orthotopic models.

Bioluminescence image of Bioware Brite HCT-116-Red-Fluc subcutaneous tumor, imaged using the IVIS Spectrum system.

*In vivo Comparison: Five million of both Bioware Brite HepG2-Red-Fluc cells and competitor luciferase transduced HepG2 cells were injected s.c. in the flank of nude mice; tumors were imaged after five weeks. Red-Fluc transduced cells generate 15 times brighter BLI signal than the corresponding tranduced cells despite similar tumor size. (Peterson, et al. 2014) Brightness varies by cell line.

Bioluminescence image of Bioware Brite U-87 MG Red-Fluc orthotopic tumor imaged using the IVIS Spectrum system.

Bioware® Brite cell lines labeled with enhanced Red-Fluc vector


HT1080-Red-Fluc Human Fibrosarcoma Cancer Cell line. BW 128092

4T1-Red-Fluc Murine Breast Cancer Cell line BW 124087

GL261-Red-Fluc Murine Glioma Cell line BW 134246

HepG2-Red-Fluc Human Hepatic Cancer cell line BW 134280

PC-3-Red-Fluc Human Prostate Cancer Cell line BW 128444

LnCaP-Red-Fluc Human Prostate Cancer Cell line BW 125055

B16-F10-Red-Fluc Murine Melanoma Cancer Cell line BW 124734

HCT-116-Red-Fluc Human Colorectal Cancer Cell line BW 124318

HT-29-Red-Fluc Human Colorectal Cancer Cell line BW 124353

Colo205-Red-Fluc Human Colorectal Cancer Cell line BW 124317

U-87 MG-Red-Fluc Human Brain Cancer Cell line, ideal for glioblastoma models BW 124577

NCI-H460-Red-Fluc Human Lung Cancer Cell line, ideal for orthotopic lung tumor models BW 124316

K-562-Red-Fluc HumanLeukemiaCellline BW 124735

BxPC3-Red-Fluc Human Pancreatic Cancer Cell BW 125058

MCF-7-lRed-Fluc Human Breast Cancer BW 119262

A549-Red-Fluc Human Lung Cancer BW 119266

LL/2-Red-Fluc Murine Lung Cancer BW 119267

SKOV3-Red-Fluc Human Ovarian Cancer BW 119276

4T1-Red-Fluc-GFP MurineBreastcancercelllineduallabeledwithLuciferaseandGFP BW128090

PC-3-Red-Fluc-GFP HumanProstatecancercelllineduallabeledwithLuciferaseandGFP BW133416

Bioware® Brite Ultra Green cell lines dual-labeled with enhanced Red-Fluc vector and Green Fluorescent ProteinProduct Product Description Catalog Number


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XenoLight Bioluminescent/Chemiluminescent Substrates

PerkinElmer offers bioluminescent substrates in two easy-to-use formats that fit your laboratory workflow for in vivo imaging.

XenoLight RediJect substrates in pre-formulated, ready-to-use kits, reduce preparation time and effort, while still delivering ultimate sensitivity and reproducibility that is critical for accurate quantitation. Optimize your work flow patterns and obtain better results by minimizing batch-to-batch variation with batch controlled lots.

LUMINESCENCE AGENTS

XenoLight RediJect Pre-formulated in PBS, batch controlled D-Luciferin (K+ salt) ready for in vivo use 770504 D-Luciferin (50 injections)

XenoLight RediJect Pre-formulated in PBS, batch controlled D-Luciferin (K+ salt) for in vivo use D-LuciferinUltra(50injections) Includesafluorescentmarkertovalidatesubstrateinjection 770505

XenoLight RediJect Pre-formulated in PBS, batch controlled Coelenterazine h for in vivo use 760506 Coelenterazine h (50 injections)

XenoLightRediJectInflammation Pre-formulatedinPBS,chemiluminescentprobeformonitoringinflammation 760535 Probe,Explorerkit(5injections)

XenoLightRediJectInflammation Pre-formulatedinPBS,chemiluminescentprobeformonitoringinflammation 760536 Probe,Standardkit(20injections)

XenoLight D-Luciferin Lyophilized bioluminescence substrate for in vivoimagingwithFireflyLuciferase 122799 (K+ Salt) (1 g)


RediFectRed-Fluc-Puromycin Lentivirusparticlescontainingred-shiftedfireflyluciferasewithpuromycinasselectionmarker CLS960002

RediFectRed-Fluc-GFP Lentivirusparticlescontainingred-shiftedfireflyluciferaseandGreenFluorescentProtein(GFP) CLS960003

RediFectGreenRenilla-Puromycin LentivirusparticlescontainingGreenRenillaluciferasewithpuromycinasselectionmarker CLS960004


RediFect lentiviral particles

RediFect™ lentiviral particles are self-inactivating, recombination incompetent lentiviral particles carrying red-shifted firefly luciferase (Luciola italica) or green-shifted Renilla luciferase (Renilla reniformis)

Also available is XenoLight RediJect Ultra, the same preformulation but with a rapidly clearing fluorescent dye to validate your substrate injection, and provide you with confidence in your data quality.

XenoLight D-Luciferin offers the same sensitivity and high performance in lyophilized form, available in gram and bulk quantities.

All PerkinElmer substrates have been optimized and validated in multiple biophotonic imaging applications including in vivo using the PerkinElmer IVIS® Imaging Systems.

transgene under control of the stable UbC promoter. Get rapid, stable and efficient transduction of a wide variety of mammalian cells including most cancer cell lines, primary, stem, and non-dividing cells.

Kinetics of XenoLight® RediJect™ D-Luciferin bioluminscence imaging in a flank 4T1 mouse breast adenocarcinoma.

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Optical in vivo imaging technology has been successfully used to non-invasively measure the spread of infection, monitor infection dynamics and determine the in vivo efficacy of antimicrobial compounds in various ID models. PerkinElmer offers various Gram positive and Gram negative bacteria labeled with bacterial Luciferase. One advantage of bacterial Luciferase is that it negates the use of an exogenous substrate like Luciferin.

E. coli EPEC WS2572 (Xen14) 119223

L. monocytogenes ATCC 23074 (Xen19) 119237 10403S(Serotype1/2awild-typestrain) (Xen32) 119238

P. aeruginosa ATCC 19660 (Xen5) 119228 PAO1 (Xen41) 119229

P. mirabilis ATCC 51286 (Xen44) 119236

S. dysenteriae 88A6205. Clinical isolate (Xen27) 119231

S. typhimurium FDA1189 (Xen33) 119235

Y. enterocolitica 91A1854 Clinical isolate (Xen24) 119232 WS2589 (Xen25) 119233

S. aureus 8325-4 (Xen8.1) 119239 ATCC 12600 (Xen29) 119240 ATCC 33591 (Xen31) 119242 ATCC 49525 (Xen36) 119243 UAMS-1 (Xen40) 119244

Bacterium Parental strain Catalog No. Bacterium Parental strain Catalog No.

OPTICAL REPORTER MICROORGANISMS

Real time non-invasive imaging of Proteus mirabilis - Xen 44 migration of UTI infection from the bladder to the kidney. Imaged using IVIS Spectrum system.

Pseudomonas aeruginosa - Xen05 biofilm infection imaged on IVIS Spectrum.

Pseudomonas aeruginosa - Xen05 imaged on IVIS Spectrum.

Results. Smarter. Faster.

With a growing emphasis on translational insight, it is more important than ever to be able to examine the molecular mechanisms of disease and translate your in vitro models into in vivo results. PerkinElmer offers leading solutions and renowned expertise in assays, imaging and informatics that will help you bring it all together. Whether working in a well, cell or animal models large or small, now you can focus on your science, gain insight sooner, and succeed faster.

Learn more at www.perkinelmer.com/invivoreagents

For a complete listing of our global offices, visit www.perkinelmer.com/ContactUs

Copyright©2010-2019,PerkinElmer,Inc.Allrightsreserved.PerkinElmer®isaregisteredtrademarkofPerkinElmer,Inc.Allothertrademarksarethepropertyoftheirrespectiveowners. 009335N_01 PKI

PerkinElmer, Inc. 940 Winter Street Waltham, MA 02451 USA P: (800) 762-4000 or (+1) 203-925-4602www.perkinelmer.com

PerkinElmer in vivo imaging agents are optimized for use in our suite of optical preclinical imaging systems.

obtain more information from your target · more complete biological assessment. in vivo....

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