nutritional metrology: the role of reference materials in improving quality of fats and oils...

Nutritional Metrology: The Role of Reference

Materials in Improving Quality of

Fats and Oils Analysis

Dr. Ibrahim Tahoun

National Institute of Standards

[email protected]

Mobile: 01002644877

1

Metrology = science of measurement

and its applications

2

Metrology Applications

Reference Materials (Preparation and Use)

Traceability and Calibration in Chemical

Measurements

Validation of Measurement Procedures

Uncertainty of Measurement Results

Statistical Treatments of Analytical Results

Quality Assurance [Internal& External]

3

Data Quality

Organizational structure

Management system

Quality manual

Document control

Qualified and trained

staff

Documented and validated

method

Calibrated and

maintained instrument

(Certified) reference material

Quality Control

PT

Quality of Measurement Results

Measurement results must be:-

Traceable to a known and recognizable set of

national and international standards.

Valid and accurate measurements depend upon

use of valid methods of analysis and use of

reference materials and establishment of adequate

quality control systems.

5

Traceability to SI Units

Traceability to the International System of Units (SI) leads to

measurement results that have three properties.

Stable

Results from measurements of the same quantity against the

same reference in one laboratory will be stable over time.

Comparable

Results from measurements of the same quantity against the

same reference in different laboratories will be comparable over

both time and space.

Absolute

Results from measurements of the same quantity against

different references will be same over both time and space.

International traceability to the SI provides all three of these

6

Reference materials Laboratory is primary metrology

laboratory (Metrology in Chemistry), it develops national

measurement standards (CRM).

Development and improvement of certification, harmonization

and compatibility of reference materials with international

standards.

Technical support to industry in everything related to

measurements, reference materials and data to establish

traceability of their measurements.


NIS

AWA

EGAC

EOS


Reference Materials

RM Laboratory Produced number of Reference materials for

food analysis:-

- Anions standard solutions

- Elemental standard solutions

- Pesticides solutions

- Vitamins in Milk Powder and Infant Formula

- Tocopherols in Edible oils

13

RM Production

Different indicator parameters are used to assess the quality of

edible fats and oils, including:-

Iodine value

Free, trans fatty acids and fatty acids profile

Heavy metals and trace elements and phosphorus content

Tocopherol and tocotrienol contents

Mono-, di-, triglyceride contents

Residual solvents

Water content

Quality of Edible Fats and Oils

Individual and total sterols contents

Saponification value

Peroxide value

Acid value and acidity

Ester value

Turbidity

Color

Flash and smoke point

Refractive index

Quality of Edible Fats and Oils

Test Method Equipment

Determination of heavy metals and trace elements BS EN ISO 5774:2001 AAS

Determination of phosphorus content BS ISO 10540-2:2003

Determination of benzo[a]pyrene ISO15302:2010 HPLC

Determination of stigmastadienes in vegetable oils BS EN ISO 15788-2:2004

Determination of tocopherol and tocotrienol contents BS EN ISO 9936:2006

Determination of residual benzene content BS ISO 14714:1998 GC and GC-MS

Determination of free and total glycerol and mono-, di-, triglyceride contents EN 14105

Determination of individual and total sterols contents BS EN ISO 2228:1999

Determination of aliphatic alcohols content BS ISO 12871:2010

Determination of total residual hexane BS EN ISO 8892:1995

Determination of water content BS ISO 11021:1999 KF

Determination of iodine value BS EN ISO 961:2000

Determination of moisture and volatile matter content BS 4325-1: 1978

Determination of saponification value BS EN ISO 657:2003

Determination of peroxide value ISO3960:2007

Determination of acid value and acidity ISO 660:2009

Determination of acid value BS ISO 1242:1999

Determination of ester value BS ISO 709:2001

Determination of Turbidity Turbidity meter

Determination of Color Lovibond

Determination of Flash and smoke point

Determination of refractive index Refractometer

min15 20 25 30 35 40 45 50 55

mAU

0

2

4

6

8

10

12

DAD1 C, Sig=298,8 Ref=360,16, TT (TOCOPHEROL 24-9-2012\TOCO 2012-09-25 11-19-15\012-0201.D)

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Vitamin E Isomers in Corn Oil by Normal and

Reversed Phase Liquid Chromatography

m in6 8 1 0 1 2 1 4

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2

4

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1 2

1 4

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Vitamin E Isomers in Peanut Oil by Normal and


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Vitamin E Isomers in Soybean Oil by Normal and


m in6 8 1 0 1 2 1 4

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Fat Soluble Vitamin in Infant Formula by Normal and


Fat Soluble Vitamin in Milk Powder by Normal and


min4 6 8 10 12 14 16 18

mAU

0

50

100

150

200

250

DAD1 C, Sig=296,8 Ref=off (WSV 2012\SIG1000077.D)

4.6

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3.6

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6

Fatty acid Composition by Gas Chromatography

Equipment and Facilities

- Gas Chromatography with variety of detectors (ECD, NPD, FID and MS).

- Gas Chromatography sample preparation and introduction systems (Static and

dynamic headspace and SPME)

LC systems to cover many types of samples

- HPLC with Diode array Detector

- Preparative LC System for sample purification and fractionation

- IC with conductivity detector for charged species


AAS equipped to cover all types of samples

- Flame

- Graphite

- Hydride

- Solid sampling


Calibration

Reference Materials

Testing

Training

28

Services Provided by RM

Laboratory

nutritional metrology: the role of reference materials in improving quality of fats and oils...

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