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Novocastra HD - HematopathologyHighly Definitive Antibodies
Trust the HD Difference – Highly Definitive Antibodies for Diagnostic Confidence• Independently benchmarked against leading equivalents by external QA*
• Establishing a new quality standard in IHC; simplifying antibody selection
*Independent analysis commissioned by Leica Biosystems and conducted by NordiQC according to the manufacturers’ instructions for use and on the corresponding staining platform.
Anaplastic Large Cell LymphomaStrong nuclear and cytoplasmic staining in the neoplastic cells.
Human Follicular LymphomaNeoplastic cells show a moderate, predominantly cytoplasmic staining reaction.
Human Diffuse Large B Cell LymphomaThe majority of neoplastic cells show a moderate to strong nuclear staining reaction.
Human ThymomaNeoplastic cells show a moderate to strong and distinct membrane staining reaction.
Human Lymph Node, T Cell LymphomaNeoplastic cells show a moderate and distinct predominantly membrane staining reaction. Occasional plasma cells show a strong staining reaction.
T Cell LymphomaMembrane staining of tumor cells.
T Cell LymphomaTumor cells show a strong membrane staining reaction.
T Cell LymphomaIntense membrane staining of tumor cells.
BOND 7 mL PA0306
Liquid 0.5 mL NCL-L-ALK
Anaplastic Lymphoma Kinase (ALK)
Clone 5A4
BOND 7 mL PA0117
Liquid 1 mL NCL-L-bcl-2
Bcl-2 Oncoprotein Clone Bcl-2/100/D5
BOND 7 mL PA0204
Liquid 1 mL NCL-L-Bcl-6-564
Bcl-6 Oncoprotein Clone LN22
BOND 7 mL PA0235
Liquid 0.5 mL NCL-L-CD1a-235
Liquid 1 mL NCL-L-CD1a-235
CD1a Clone MTB1
CD3 Clone LN10
BOND 7 mL PA0553
BOND 30 mL PA0122
Liquid 1 mL NCL-L-CD3-565
CD4 Clone 4B12
BOND 7 mL PA0427
Liquid 0.5 mL NCL-L-CD4-368
Liquid 1 mL NCL-L-CD4-368
BOND 7 mL PA0168
Liquid 0.5 mL NCL-L-CD5-4C7
Liquid 1 mL NCL-L-CD5-4C7
CD5 Clone 4C7 CD7 Clone
LP15
BOND 7 mL PA0266
Liquid 1 mL NCL-L-CD7-580
Novocastra HD - Hematopathology
BOND 7 mL PA0843
Liquid 0.5 mL NCL-L-CD19-163
Liquid 1 mL NCL-L-CD19-163
Human Lymph Node, T Cell LymphomaNeoplastic cells show a moderate to strong, distinct membrane staining reaction.
Human Lymphoblastic LymphomaIntense membrane staining of neoplastic lymphoid cells.
Human Hairy Cell LeukemiaMembrane staining of malignant cells.
Hodgkin’s Disease, Mixed CellularityMixed cellular membrane staining and characteristic staining of paranuclear hofs of Reed Sternberg cells.
Pre B Acute Lymphoblastic LymphomaNeoplastic cells show a moderate to strong predominantly membrane staining reaction.
Mantle Cell LymphomaIntense membrane staining of malignant lymphocytes.
Follicular B Cell LymphomaFollicular B cell lymphoma showing a strong membrane staining reaction.
Human TonsilNK cells and CD4/CD8 double positive T cells show a weak to moderate and distinct membrane staining reaction while the majority of lymphocytes are unstained.
Mantle Cell LymphomaNeoplastic cells show a moderate and distinct predominantly membrane staining reaction. Occasional plasma cells show a strong staining reaction.
Hodgkin’s LymphomaNeoplastic cells show a moderate and distinct predominantly membrane staining reaction. Occasional plasma cells show a strong staining reaction.
Human LymphomaMembrane staining of endothelial cells.
Acute Myeloid LeukemiaNeoplastic cells show a moderate and distinct predominantly membrane staining reaction. Occasional plasma cells, as expected, show a strong staining reaction.
CD19 Clone BT51E
CD8 Clone 4B11
BOND 7 mL PA0183
Liquid 0.5 mL NCL-L-CD8-4B11
BOND 7 mL PA0270
BOND 30 mL PA0131
Liquid 1 mL NCL-L-CD10-270
CD10 Clone 56C6
BOND 7 mL PA0554
Liquid 1 mL NCL-L-CD11c-563
BOND 7 mL PA0473 NEW
CD11c Clone 5D11 CD15 Clone
MMA
CD20 Clone L26
BOND 7 mL PA0200
BOND 30 mL PA0359
Liquid 0.5 mL NCL-L-CD20-L26
Liquid 1 mL NCL-L-CD20-L26
BOND 7 mL PA0169
Liquid 0.5 mL NCL-L-CD23-1B12
Liquid 1 mL NCL-L-CD23-1B12
CD23 Clone 1B12
BOND 7 mL PA0790
Liquid 1 mL NCL-L-CD30-591
CD30 Clone JCM182
CD31 Clone JC70A
BOND 7 mL PA0414
Liquid 1 mL NCL-L-CD31-607
BOND 7 mL PA0555
Liquid 1 mL NCL-L-CD33
CD33 Clone PWS44
BOND 7 mL PA0042
Liquid 0.5 mL NCL-L-LCA
Liquid 1 mL NCL-L-LCA
CD45 Clone X16/99
BOND 7 mL PA0191
Liquid 1 mL NCL-L-CD56-504
CD56 Clone CD564
Human AppendixIntense staining of plasma cells and secreted immunoglobulin A.
Myeloid SarcomaSelective medium to strong staining of neoplastic cells.
Mantle Cell LymphomaMantle cell tumor cells show moderate staining, with a stronger reaction seen in heavy chain expressing plasma cells.
Control Tissue, Human ColonIntense staining of the epithelium in the crypts.
Human Mantle Cell LymphomaNuclear staining of malignant pre-germinal B lymphocytes.
Human TonsilGerminal centre macrophages show a strong cytoplasmic staining reaction, while the interfollicular macrophages show correct weak to moderate staining reaction.
PlasmacytomaIntense membrane staining of tumor cells.
Human Gastrointestinal Stromal TumorIntense membrane staining of tumor cells.
Human Diffuse Large B Cell LymphomaMembrane staining of tumor cells.
CD68 Clone 514H12
BOND 7 mL PA0273
Liquid 1 mL NCL-L-CD68
BOND 7 mL PA0007
Liquid 1 mL NCL-L-CD117-032
BOND 7 mL PA0599 NEW
CD117 (c-kit Oncoprotein)
Clone EP10 CD79a Clone
JCB117
BOND 7 mL PA0088
CD138 Clone MI15
Liquid 1 mL NCL-L-CD163 NEW
CD163 Clone 10D6 Cytokeratin 20 Clone
Ks20.8 Cyclin D1 Clone EP12
BOND 7 mL PA0022
BOND 30 mL PA0037
Liquid 0.5 mL NCL-L-CK20
Liquid 1 mL NCL-L-CK20
BOND 7 mL PA0046 NEW
Immunoglobulin A Clone N1CLA
Liquid 1 mL NCL-L-IgA
Immunoglobulin D Clone DRN1C
BOND 7 mL PA0061 He Only
Liquid 1 mL NCL-L-IgD
Invasive Breast CarcinomaIntense staining of invasive tumor cells.
Cytokeratin 7 Clone RN7
BOND 7 mL PA0942
BOND 30 mL PA0138
Liquid 1 mL NCL-L-CK7-560
Human Skin, Kaposi’s SarcomaNeoplastic cells show moderate cytoplasmic staining, while normal endothelial cells show strong staining.
Factor VIII related antigen (von Willebrand Factor)
Clone 36B11
BOND 7 mL PA0055 NEW
Liquid 1 mL NCL-L-vWF
Immunoglobulin G Clone RWP49
BOND 7 mL PA0905 NEW
Liquid 1 mL NCL-L-IgG
Human TonsilCytoplasmic staining of the plasma myeloma cells.
LeicaBiosystems.com
Novocastra HDHighly Definitive Antibodies
Benefits of BOND Ready-to-Use antibodies
✓ Optimized for staining performance on BOND
✓ Validated antibody concentrations
✓ Ease of use: just plug and play
✓ Increase standardization through automation
✓ Eliminate variability in antibody preparation
Benefits of concentrated liquid antibodies
✓ Flexibility of use on various platforms1
✓ Adaptable to a variety of detection systems1
✓ Adjustable concentration to suit user’s tissue1
✓ Economical option that retains confidence in clone selection
1. The performance of antibodies should be validated when used with automated platforms or manual staining systems other than stated on Instructions for Use.
Improve workflow efficiency and streamline validation.
95.11988 Rev F · 03/2016 · Copyright©2016 Leica Biosystems Newcastle Ltd. All rights reserved. LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH. Novocastra and BOND are trademarks of Leica Biosystems and its affiliates. Other logos, product and/or company names might be trademarks of their respective owners. The EP clones have been created by Epitomics Inc., using Epitomics’ proprietary rabbit monoclonal antibody technology covered under Patent No.’s 5, 675, 063 and 7, 402, 409.
Pre B Cell Acute Lymphoblastic LeukemiaTumor cells show a strong, nuclear staining reaction.
Pre B Cell Acute Lymphoblastic LeukemiaNeoplastic cells show a strong and distinct nuclear staining reaction.
Human Kidney, Wilms’ TumorNuclear staining of invasive tumor cells.
BOND 7 mL PA0552
Liquid 1 mL NCL-L-PAX-5
Pax-5 Clone 1EW
BOND 7 mL PA0339
Liquid 0.5 mL NCL-L-TdT-339
Liquid 1 mL NCL-L-TdT-339
Terminal Deoxynucleotidyl Transferase (TdT)
Clone SEN28
BOND 7 mL PA0562
Liquid 1 mL NCL-L-WT1-562
Wilms’ Tumor Clone WT49
BOND 7 mL PA0129
Liquid 1 mL NCL-L-MUM1
Multiple Myeloma Oncogene 1 (MUM-1)
Clone EAU32
Hodgkin’s LymphomaA moderate to strong predominantly nuclear staining reaction is seen in the Reed-Sternberg cells and plasma cells.
B Cell Chronic Lymphocytic LeukemiaNeoplastic cells show a moderate and distinct predominantly membrane staining reaction. Occasional plasma cells show a strong staining reaction.
High Grade B Cell Immunoblastic LymphomaIntense nuclear staining in proliferating B cells.
Kappa Light Chain Clone CH15 Ki67 Clone
MM1
BOND 7 mL PA0606 He Only
Liquid 1 mL NCL-L-KAP-581
BOND 7 mL PA0118
BOND 30 mL PA0410
Liquid 1 mL NCL-L-Ki67-MM1
Lambda Light Chain Clone SHL53
BOND 7 mL PA0570 He Only
Liquid 1 mL NCL-L-LAM-578
B Cell Chronic Lymphocytic LeukemiaNeoplastic cells show a moderate and distinct predominantly membrane staining reaction. Occasional plasma cells show a strong staining reaction.
Immunoglobulin M Clone 8HC
BOND 7 mL PA0278 NEW
Liquid 1 mL NCL-L-IgM NEW
Normal Human LarynxIntense membrane staining of lymphocytes.