Short-neck clam, Ruditapes philippinarum cultureusing Porphyra spheroplast in closed recirculation system

with special reference to water quality monitoringNovi Arisman1, Takao Yoshimatsu1, Mohammad Nakid Dad Khan1,

A.M. Shahabuddin1, Toshiyoshi Araki1

What is Porphyra?

Porphyra spp., including P. yezoensis as its representative species, is among the nutritious edible seaweeds. It usually to be used as mainly as a luxury food, wrapped around a small handful of rice in sushi.

The experiment aimed to clarify the suitability and availability of feeds as a continual development of new culture feed for aquatic animal from Porphya prepared by enzymatic digestion.

Objective

METHOD

How to prepare Porphyra spheroplast (PS)?

Porphyra spp. P. yozoensis

Enzymatic digestion

Centrifugation

Supernatant Precipitate

Removing cell wall,enhance the digestibility

Liquid mixture

Freeze drying

Spray drying

FDS

SDS

ETN

PS

PS + oligosaccharides This three kinds of PS were used as the experimental

feed for sort-neck clam, d in the closed recirculation system.

FDS SDS ETN

Why:•Good taste & high nutritive value•High demandProblem:•Highly dependent on live micro algae (diatom: Chaetoceros calcitrans) – Integrated algal culture facility

Porphyra spheroplast has a great potential opportunity to be implemented as a substitute for live micro algae, base on the growth, survival, and water quality. Special attention for ETN, this type of PS are predominate as a simpler and less consuming time that particularly beneficial in order to maximize cost-effectiveness.

RESULT

Feed Treatments

Shell Length(mm)

Shell Width(mm)

Shell Depth(mm)

Carcass wet weight (g)

Carcass Ratio(CR)

Condition Index (CI)

FDS 30.65 ± 0.44 21.34 ± 0.53 14.63 ± 0.13 0.97 ± 0.03a 20.48 ± 0.55 10.22 ± 0.32SDS 30.65 ± 0.15 22.16 ± 1.44 14.51 ± 0.10 0.95 ± 0.05 20.77 ± 0.57 10.15 ± 0.77ETN 30.51 ± 0.47 20.89 ± 0.04 14.41 ± 0.17 0.90 ± 0.03a 19.81 ± 0.94 9.96 ± 0.63

Rearing results of short-neck clam, Ruditapes philippinarum fed with different type of Porphyra spheroplast in closed recirculation system for 30 days

Parameter Initial FDS SDS ETN

Temperature (0C) 17.97 ± 0.04 17.97 ± 0.04 17.97 ± 0.04 17.97 ± 0.04

Salinity (psu) 30 30 30 30

pH 8.10 7.91 ± 0.08 7.93 ± 0.09 7.93 ± 0.09

DO (ppm) 6.4 6.91 ± 0.12 7.03 ± 0.16 7.17 ± 0.22

Nitrogen, Ammonia (mg/L) 0.06 0.17 ± 0.15 0.13 ± 0.06 0.11 ± 0.04

Nitrogen, Nitrite (mg/L) 0.225 0.234 ± 0.02 0.205 ± 0.03 0.233 ± 0.04

Nitrogen, Nitrate (mg/L) 2.30 3.96 ± 2.10 3.67 ± 2.04 3.51 ± 2.04

Phosphorus (mg/L) 0.32 1.65 ± 1.11 1.72 ± 1.07 1.55 ± 0.92

Hardness, total (mg/L) 4975 5511.00 ± 365.31 5584.33 ± 493.87 5667.5 ± 384.81

Alkalinity (mg/L) 140 134.00 ± 16.94 151.67 ± 24.83 144.5 ± 23.51

Cloride (mg/L) 4462.25 3193.25 ± 779.37 3178.42 ± 748.60 3181.25 ± 666.51

Carbon dioxide (mg/L) 2.5 2.47 ± 1.00 2.30 ± 1.22 2.36 ± 0.76

Mean of water quality parameter

Conclusion

1Graduate School of Bioresources, Mie University, Tsu, Mie 514-8507, Japan

Kalla, A., Yoshimatsu, T., Araki, T., Zhang, D. M., Yamamoto, T., & Sakammoto, S. (2008). Use of Porphyra spheroplasts as feed additive for red sea bream. Fisheries Science, 104–108.

Kalla, A., Yoshimatsu, T., Higano, J., Umemoto, Y., Sakamoto, S., & Araki, T. (2008). Novel Diet for Short-Neck Clam by Using Porphyra Spheroplasts. Aquaculture Science, 56(3), 465-466

Khan, M. N., Yoshimatsu, T., Kalla, A., Ishihi, Y., Araki, T., & Sakamoto, S. (2009). Effect of Dif ferent Particle Size of Porphyra Spheroplasts Diet for Short-neck Clam. Aquaculture Science, 57(1), 31-38.

Reference