novel technologies in mass rearing of beneficial arthropods · anagyrus field trials in citrus |...
TRANSCRIPT
![Page 1: Novel Technologies in Mass Rearing of Beneficial Arthropods · Anagyrus field trials in citrus | Western Cape, S.A Augmentation with Anagyrus sp. nr pseudococci should be conducted](https://reader033.vdocuments.site/reader033/viewer/2022042314/5f020c4b7e708231d402514e/html5/thumbnails/1.jpg)
Novel Technologies in Mass Rearing of
Beneficial Arthropods
Shimon Steinberg, BioBee Sde Eliyahu Ltd.ISRAEL ABIM, Basel, October 2013
![Page 2: Novel Technologies in Mass Rearing of Beneficial Arthropods · Anagyrus field trials in citrus | Western Cape, S.A Augmentation with Anagyrus sp. nr pseudococci should be conducted](https://reader033.vdocuments.site/reader033/viewer/2022042314/5f020c4b7e708231d402514e/html5/thumbnails/2.jpg)
Macrobial natural enemies for biological pest control
BioBee Sde Eliyahu Ltd. Produces, markets and implementscomprehensive solutions by means of:
Bumblebees for natural pollination
Sterile Mediterranean fruit flies for medfly control using Sterile Insect Technique (SIT)
Biopesticides - To complement the natural enemy’s package
![Page 3: Novel Technologies in Mass Rearing of Beneficial Arthropods · Anagyrus field trials in citrus | Western Cape, S.A Augmentation with Anagyrus sp. nr pseudococci should be conducted](https://reader033.vdocuments.site/reader033/viewer/2022042314/5f020c4b7e708231d402514e/html5/thumbnails/3.jpg)
BioBee
• Established 1983
• Two hundred employees in Israel and
in 3 subsidiaries
• The majority of the produce is
exported to 100 destinations world-
wide
![Page 4: Novel Technologies in Mass Rearing of Beneficial Arthropods · Anagyrus field trials in citrus | Western Cape, S.A Augmentation with Anagyrus sp. nr pseudococci should be conducted](https://reader033.vdocuments.site/reader033/viewer/2022042314/5f020c4b7e708231d402514e/html5/thumbnails/4.jpg)
BioBee
![Page 5: Novel Technologies in Mass Rearing of Beneficial Arthropods · Anagyrus field trials in citrus | Western Cape, S.A Augmentation with Anagyrus sp. nr pseudococci should be conducted](https://reader033.vdocuments.site/reader033/viewer/2022042314/5f020c4b7e708231d402514e/html5/thumbnails/5.jpg)
Production of P. persimilis
Off-Plant Production of the Predatory Mite:
Phytoseiulus persimilis
![Page 6: Novel Technologies in Mass Rearing of Beneficial Arthropods · Anagyrus field trials in citrus | Western Cape, S.A Augmentation with Anagyrus sp. nr pseudococci should be conducted](https://reader033.vdocuments.site/reader033/viewer/2022042314/5f020c4b7e708231d402514e/html5/thumbnails/6.jpg)
Production of P. persimilis
Modified Tri-trophic System
P. persimilisSpider-miteBean
![Page 7: Novel Technologies in Mass Rearing of Beneficial Arthropods · Anagyrus field trials in citrus | Western Cape, S.A Augmentation with Anagyrus sp. nr pseudococci should be conducted](https://reader033.vdocuments.site/reader033/viewer/2022042314/5f020c4b7e708231d402514e/html5/thumbnails/7.jpg)
Production of P. persimilis
• Indoor (=climate room) controlled rearing system
![Page 8: Novel Technologies in Mass Rearing of Beneficial Arthropods · Anagyrus field trials in citrus | Western Cape, S.A Augmentation with Anagyrus sp. nr pseudococci should be conducted](https://reader033.vdocuments.site/reader033/viewer/2022042314/5f020c4b7e708231d402514e/html5/thumbnails/8.jpg)
Production of P. persimilis
• Indoor (=climate room) controlled rearing system
• The product: age distribution includes 30-50% eggs
![Page 9: Novel Technologies in Mass Rearing of Beneficial Arthropods · Anagyrus field trials in citrus | Western Cape, S.A Augmentation with Anagyrus sp. nr pseudococci should be conducted](https://reader033.vdocuments.site/reader033/viewer/2022042314/5f020c4b7e708231d402514e/html5/thumbnails/9.jpg)
Production of P. persimilis
• Indoor (=climate room) controlled rearing system
• The product: age distribution includes 30-50% eggs
• The product: well fed gravid females capable of
surviving storage and transport and establish
rapidly in the field
![Page 10: Novel Technologies in Mass Rearing of Beneficial Arthropods · Anagyrus field trials in citrus | Western Cape, S.A Augmentation with Anagyrus sp. nr pseudococci should be conducted](https://reader033.vdocuments.site/reader033/viewer/2022042314/5f020c4b7e708231d402514e/html5/thumbnails/10.jpg)
P. persimilis rate of establishment
Tomato | 2012
![Page 11: Novel Technologies in Mass Rearing of Beneficial Arthropods · Anagyrus field trials in citrus | Western Cape, S.A Augmentation with Anagyrus sp. nr pseudococci should be conducted](https://reader033.vdocuments.site/reader033/viewer/2022042314/5f020c4b7e708231d402514e/html5/thumbnails/11.jpg)
Spider mites population dynamics
Tomato | 2012
![Page 12: Novel Technologies in Mass Rearing of Beneficial Arthropods · Anagyrus field trials in citrus | Western Cape, S.A Augmentation with Anagyrus sp. nr pseudococci should be conducted](https://reader033.vdocuments.site/reader033/viewer/2022042314/5f020c4b7e708231d402514e/html5/thumbnails/12.jpg)
Prey/predator ratio
Tomato | 2012
![Page 13: Novel Technologies in Mass Rearing of Beneficial Arthropods · Anagyrus field trials in citrus | Western Cape, S.A Augmentation with Anagyrus sp. nr pseudococci should be conducted](https://reader033.vdocuments.site/reader033/viewer/2022042314/5f020c4b7e708231d402514e/html5/thumbnails/13.jpg)
BugFeed®
Inactivated Frozen Medfly Eggs
![Page 14: Novel Technologies in Mass Rearing of Beneficial Arthropods · Anagyrus field trials in citrus | Western Cape, S.A Augmentation with Anagyrus sp. nr pseudococci should be conducted](https://reader033.vdocuments.site/reader033/viewer/2022042314/5f020c4b7e708231d402514e/html5/thumbnails/14.jpg)
BugFeed®
![Page 15: Novel Technologies in Mass Rearing of Beneficial Arthropods · Anagyrus field trials in citrus | Western Cape, S.A Augmentation with Anagyrus sp. nr pseudococci should be conducted](https://reader033.vdocuments.site/reader033/viewer/2022042314/5f020c4b7e708231d402514e/html5/thumbnails/15.jpg)
• Alternative to Ephestia eggs as feed for mass
rearing of predatory bugs, predatory beetles,
others?
BugFeed®
![Page 16: Novel Technologies in Mass Rearing of Beneficial Arthropods · Anagyrus field trials in citrus | Western Cape, S.A Augmentation with Anagyrus sp. nr pseudococci should be conducted](https://reader033.vdocuments.site/reader033/viewer/2022042314/5f020c4b7e708231d402514e/html5/thumbnails/16.jpg)
• Alternative to Ephestia eggs as feed for mass
rearing of predatory bugs, predatory beetles,
others?
• One year storage capacity
BugFeed®
![Page 17: Novel Technologies in Mass Rearing of Beneficial Arthropods · Anagyrus field trials in citrus | Western Cape, S.A Augmentation with Anagyrus sp. nr pseudococci should be conducted](https://reader033.vdocuments.site/reader033/viewer/2022042314/5f020c4b7e708231d402514e/html5/thumbnails/17.jpg)
• Alternative to Ephestia eggs as feed for mass
rearing of predatory bugs, predatory beetles,
others?
• One year storage capacity
• Patent granted. Product on sale
BugFeed®
![Page 18: Novel Technologies in Mass Rearing of Beneficial Arthropods · Anagyrus field trials in citrus | Western Cape, S.A Augmentation with Anagyrus sp. nr pseudococci should be conducted](https://reader033.vdocuments.site/reader033/viewer/2022042314/5f020c4b7e708231d402514e/html5/thumbnails/18.jpg)
BugFeed® | as Good as Ephestia Eggs
Orius laevigatus mass rearing
0
10000
20000
30000
40000
50000
Ephestia eggs 8 months stored
BugFeed®
Mixed diet
No. of
indiv
idual
s co
llec
ted
Diet
Low feeding
High feeding
0
2
4
6
8
10
Ephestia eggs BugFeed®
% M
ort
alit
y
Diet
High feeding
Low feeding
![Page 19: Novel Technologies in Mass Rearing of Beneficial Arthropods · Anagyrus field trials in citrus | Western Cape, S.A Augmentation with Anagyrus sp. nr pseudococci should be conducted](https://reader033.vdocuments.site/reader033/viewer/2022042314/5f020c4b7e708231d402514e/html5/thumbnails/19.jpg)
Mealybug Parasitoid’s Mummies
Mealybugs’ Mummies
Anagyrus pseudococciLeptomastix algirica
![Page 20: Novel Technologies in Mass Rearing of Beneficial Arthropods · Anagyrus field trials in citrus | Western Cape, S.A Augmentation with Anagyrus sp. nr pseudococci should be conducted](https://reader033.vdocuments.site/reader033/viewer/2022042314/5f020c4b7e708231d402514e/html5/thumbnails/20.jpg)
Mealybugs’ Mummies
![Page 21: Novel Technologies in Mass Rearing of Beneficial Arthropods · Anagyrus field trials in citrus | Western Cape, S.A Augmentation with Anagyrus sp. nr pseudococci should be conducted](https://reader033.vdocuments.site/reader033/viewer/2022042314/5f020c4b7e708231d402514e/html5/thumbnails/21.jpg)
Mealybugs’ Mummies
• A robust rearing system that can be applied to a number of mealybug parasitoids
![Page 22: Novel Technologies in Mass Rearing of Beneficial Arthropods · Anagyrus field trials in citrus | Western Cape, S.A Augmentation with Anagyrus sp. nr pseudococci should be conducted](https://reader033.vdocuments.site/reader033/viewer/2022042314/5f020c4b7e708231d402514e/html5/thumbnails/22.jpg)
Mealybugs’ Mummies
• A robust rearing system that can be applied to a number of mealybug parasitoids
• A breakthrough in delivery of mealybugparasitoids’ commercial products to the field
![Page 23: Novel Technologies in Mass Rearing of Beneficial Arthropods · Anagyrus field trials in citrus | Western Cape, S.A Augmentation with Anagyrus sp. nr pseudococci should be conducted](https://reader033.vdocuments.site/reader033/viewer/2022042314/5f020c4b7e708231d402514e/html5/thumbnails/23.jpg)
Mealybugs’ Mummies
• A robust rearing system that can be applied to a number of mealybug parasitoids
• A breakthrough in delivery of mealybugparasitoids’ commercial products to the field
• Patent granted (national phase). Two products on sale
![Page 24: Novel Technologies in Mass Rearing of Beneficial Arthropods · Anagyrus field trials in citrus | Western Cape, S.A Augmentation with Anagyrus sp. nr pseudococci should be conducted](https://reader033.vdocuments.site/reader033/viewer/2022042314/5f020c4b7e708231d402514e/html5/thumbnails/24.jpg)
Mealybugs’ Mummies
R² = 0.9167
R² = 0.8949
1
2
3
4
5
6
7
8
9
0
10
20
30
40
50
60
70
80
90
100
0 5 10 15 20 25 30
Avera
ge
eg
gs
/fem
ale
Em
erg
en
ce r
ate
(%
)
storage (days)
Anagyrus pseudococci(a) Emergence rate
(b) Fertility
R² = 0.9872
R² = 0.9832
1
2
3
4
5
6
7
8
9
10
0
10
20
30
40
50
60
70
80
90
100
0 5 10 15 20 25 30 35 40 45A
vera
ge
eg
gs
/fem
ale
Em
erg
en
ce r
ate
(%
)
Storage (days)
Leptomastix algirica
(a) Emergence rate
(b) Fertility
Post-storage capacity | @12°C
![Page 25: Novel Technologies in Mass Rearing of Beneficial Arthropods · Anagyrus field trials in citrus | Western Cape, S.A Augmentation with Anagyrus sp. nr pseudococci should be conducted](https://reader033.vdocuments.site/reader033/viewer/2022042314/5f020c4b7e708231d402514e/html5/thumbnails/25.jpg)
Mealybugs’ Mummies
0.05.0
10.015.020.025.030.035.040.0
15
.11
.20
11
29
.11
.20
11
12
.12
.20
11
29
.12
.20
12
14
.01
.20
12
28
.1.2
01
2
13
.2.2
01
2
5.3
.20
12
19
.3.2
01
2
7.0
4.2
01
2
21
.04
.20
12
% f
ruit
infe
ste
d
Robyn - % fruit infestation by mealybug
Control
2500/ha
5000/ ha
Moore et al. 2012
Anagyrus field trials in citrus | Western Cape, S.A
![Page 26: Novel Technologies in Mass Rearing of Beneficial Arthropods · Anagyrus field trials in citrus | Western Cape, S.A Augmentation with Anagyrus sp. nr pseudococci should be conducted](https://reader033.vdocuments.site/reader033/viewer/2022042314/5f020c4b7e708231d402514e/html5/thumbnails/26.jpg)
Mealybugs’ Mummies
Moore et al. 2012
Anagyrus field trials in citrus | Western Cape, S.A
Augmentation with Anagyrus sp. nrpseudococci should be conducted at a rate ofat least 5000 parasitoids per ha in the firstyear, and possibly a lesser quantity from thesecond year onwards.
Conclusion
![Page 27: Novel Technologies in Mass Rearing of Beneficial Arthropods · Anagyrus field trials in citrus | Western Cape, S.A Augmentation with Anagyrus sp. nr pseudococci should be conducted](https://reader033.vdocuments.site/reader033/viewer/2022042314/5f020c4b7e708231d402514e/html5/thumbnails/27.jpg)
Take Home Message
Mass rearing of macrobial biocontrol agents is an everlasting challenge
BioBee has developed novel rearing techniques which contribute significantly to its commercial products’ Availability-Consistency-Quality
![Page 28: Novel Technologies in Mass Rearing of Beneficial Arthropods · Anagyrus field trials in citrus | Western Cape, S.A Augmentation with Anagyrus sp. nr pseudococci should be conducted](https://reader033.vdocuments.site/reader033/viewer/2022042314/5f020c4b7e708231d402514e/html5/thumbnails/28.jpg)
Thank you!
www.biobee.com