Vot. 268. No. 3 The Journal of J a ~ u a ~ 25,1993

Biological Chemistry Copyright 0 1993 by the American Society for Biochemistry and Molecular Biology, Inc.

428 East Preston St., Baltimore, MD 21202 U.S.A.

CONTENTS*

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MINIREVIEW The MARCKS family of cellular protein kinase C sub- strates. Perry J . B lac~~hear

COMMUNICATIONS Promoter activity of human renin 5'-flanking DNA sequences is activated by the pituitary-specific tran- scription factor Pit-1. Jidong Sun, Carole Oddoux, Amy Lazarus, Matthew 7'. Gilbert, and Daniel I? Catantaro

Cloning and expression of a mammalian Na+/amino acid cotransporter with sequence similarity to Na+/glucose cotransporters. Cheng-Te Kong, Shaw-Fang Yet, and Julia E. Lever

Disruption of potential sites for N-linked glycosylation does not impair hormone binding to the lutropin/cho- riogonadotropin receptor if Asn-173 is left intact. Xuebo Liu, David Davis, and Deborah L. Segaloff

Small heat shock proteins are molecular chaperones. Ursula Jakob, Matthias Gaestel, Katrin Engel, and Johonnes Buchner

The biological relevance of the binding of calcium ions by inositol phosphates. Brian M. Luttrell

ARTICLES

erties of Munducu sextu apolipophorin 111. Robert 0. Conformational, thermodynamic, and stability prop-

Ryan, Kim Oikawa, and Cyr{l M. Kay

Evidence that energization of the chloroplast ATP syn- thase favors ATP formation at the tight binding cata- lytic site and increases the affinity for ADP at another catalytic site. Jun-Mei Zhou and Paul D. Boyer

Studies on the mechanism of oxidative phosphoryla- tion. Different effects of FO inhibitors on unisite and multisite ATP hydrolysis by bovine submitochondrial particles. Akemi Matsuno-Yagi and Youssef Hatefi

Evidence for specific base catalysis in N-dealkylation

peroxidase. Differences in rates of deprotonation of reactions catalyzed by cytochrome P450 and chloro-

aminium radicals as an explanation for high kinetic hydrogen isotope effects observed with peroxidases. Osamu Okazaki and F. Peter Guengerich

pH-dependent stability and membrane interaction of the pore-forming domain of colicin A. Arturo Muga, Juan M. Gonzalez-Manas, Jeremy H. Lakey, Franc Pattus, and Witold K. Surewicz

sites which accelerates catalysis is responsible for ap- Slow binding of ATP to noncatalytic nucleotide binding

parent negative cooperativity exhibited by the bovine mitochondrial F,-ATPase. Jean-Michel Jault and William S. Allison

Cell penetration of diphtheria toxin. Reduction of the interchain disulfide bridge is the rate-limiting step of

puoli, Marta Murgia, and Cesare Montecucco translocation in the cytosol. Emanuele Papini, Rino Rap-

Genetic and immunological analyses of the cyanobac- terium Synechocystis sp. PCC 6803 show that the pro- tein encoded by the psbJ gene regulates the number of photosystem I1 centers in thylakoid membranes. Lisbet K. Lind, Vipula K. Shukla, Karin J. Nyhus, and Himadri B. Pakrasi

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Growth factors that repress myoblast differentiation

Francesca Cole, Thomas M. Fasy, Sunkara S. Rao, Mary Anne sustain phosphorylation of a specific site on histone H1.

de Peralta, and D. Stave Kohtz

Histamine induces a gene-specific synthesis regulation of secretogranin I1 but not of chromogranin A and B in chromaffin cells in a calcium-dependent manner. Jo- hann W. Bauer, Rudolf Kirchmair, Claudia Egger, and Reiner Fisc~r-Colbrie

Mutations of the molecular chaperone protein SecB which alter the interaction between SecB and maltose- binding protein. Pamela M. Gannon and Carol A. Kumamoto

The number of amphipathic a-helical segments of apo- lipoproteins A-I, E, and A-IV determines the size and functional properties of their reconstituted lipoprotein particles. Ana Jonas, Armin Steinmetz, and Lisa Churgay

Chemical modification of bacteriophage T4 deoxynu- cleotide kinase. Evidence of a single catalytic region. George S. Brush and Maurice J. Bessman

cyclic AMP, and dexamethasone on lysosomal proteol- Effects of intracellular amino acid concentrations,

Pie t j~n J. E. B ~ m m ~ r t , Dum Zonneve~ , Alfred J. Meijer, ysis in primary cultures of perinatal rat hepatocytes.

and Wouter H. Lamers

a- and 8-xylosides alter glycolipid synthesis in human

Freeze, Deepk Sampath, and Ajit Varki melanoma and Chinese hamster ovary cells. Hudson H.

Identification of vicinal thiols of phosphoenolpyruvate carboxykinase (GTP). Cristina T. Lewis, Jerome M. Seyer, Robert G. Cmsell, and Gerald M. Carlson

Horseradish peroxidase-catalyzed two-electron oxida- tions. Oxidation of iodide, thioanisoles, and phenols a t distinct sites. Robert Z. Harris, Sherri L. Newmyer, and Paul R. Ortiz de Montellano

The Streptococcus sunguis platelet aggregation-asso- ciated protein. identification and characterization of the minimal platelet-interactive domain. Pamela R. Er- ickson and Mark C. Herzberg

Repair of individual DNA strands in the hamster di- hydrofolate reductase gene after treatment with ultra- violet light, alkylating agents, and cisplatin. Alfred May, Rodney S. Nairn, Diane S. Okumoto, Karsten W ~ s e r ~ a n n , Tinna Stevnsner, Jennifer C. Jones, and Vilhelm A. Bohr

Phycobilins of cryptophycean algae. Novel linkage of dihydrobiliverdin in a phycoerythrin 555 and a phy- cocyanin 645. David E. Wemmer, Gary J . Wedemayer, and Alexander N. Glazer

Uptake of high density lipoprotein cholesterol ester by HepG2 cells involves apolipoprotein E localized on the cell surface. Lorraine Leblond and Yues L. Marcel

Isozyme-specific modules on human aldolase A mole- cule. Isozyme group-specific sequences 1 and 4 are required for showing characteristics as aldolase A. Ki- yohisa Motoki, Yoshihiko Kitajima, and Katsuji Hori

Precise location of the Cu(I1)-inhibitory binding site in higher plant and bacterial photosynthetic reaction cen- ters as probed by light-induced absorption changes. Inmaculada Yruela, Miguel Alfonso, Iriaki Ortiz de Zarate, Guillermo Montoya, and Rafael Picorel

* The CONTENTS arranged by Subject Categories will be found immediately following these CONTENTS. Full l n s t ~ u c t i o ~ ~ to A u t ~ r s will be found in (1993) J. Bwl. Chem. 268, 745-753, and reprints may be obtained from the editorial office.

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Cooperative phenomena in binding and activation of

Ahmed Bouhss, Euelyne Krin, Helene Munier, Anne-Marie Bordetella pertussis adenylate cyclase by calmodulin.

Gilles, Antoine Danchin, Philippe Gloser, and Octauian Bkrzu

Characterization of a synthetic calmodulin-binding peptide derived from Bacillus anthracis adenylate cy- clase. Helene Munier, Francisco J . Blanco, Benedicte Pr6- cheur, Eric Diesis, Jose L. Nieio, Constantin T. Craescu, and Octauian Bkrzu

Purification and characterization of membrane-bound chitin synthase. Sachiko Machida and Michihiko Saito

with soybean peroxygenase. Elizabeth Blie, Allan L. Wil- Mechanism of reaction of fatty acid hydroperoxides

cox, Lawrence J. Marnett, and Francis Schuber

Influenza hemagglutinin-mediated membrane fusion does not involve inverted phase lipid intermediates. Toon Stegmann

A luteinizing hormone receptor with a severely trun- cated cytoplasmic tail (LHR-ct628) desensitizes to the same degree as the full-length receptor. X i Zhu, Thomas Gudermann, Marie1 Birnbaumer, and Lutz Birnbaumer

Physiological role of NhaB, a specific Na+/H+ anti- porter in Escherichia coli. Elhanan Pinner, Yaniu Kotler, Etana Padan, and Shimon Schuldiner

Hydrolysis of short acyl chain inositol lipids by phos- pholipase C-6,. Mario J . Rebecchi, Robert Eberhardi, Tracy Delaney, Shaukat Ali, and Robert Bittman

Cumulative effect of double-site mutations of human epidermal growth factor on receptor binding. Stephen R. Campion, Mary K. Geck, and Salil K. Niyogi

Caz+-dependent and Caz+-independent isozymes of pro-

rat basophilic RBL-2H3 cells. Reconstitution of secre- tein kinase C mediate exocytosis in antigen-stimulated

tory responses with Ca2+ and purified isozymes in washed permeabilized cells. Koichiro Ozawa, Zoltan Szal- lasi, Marcel0 G. Kazanietz, Peter M. Blumberg, HaraM Mis- chak, J . Frederic Mushinski, and Michael A. Beauen

An N-terminal glycosylation signal on cytochrome P450 is restricted to the endoplasmic reticulum in a luminal orientation. Elzbieta Szczesna-Skorupa and Byron Kemper

The prohormone convertases PC1 and PC2 mediate distinct endoproteolytic cleavages in a strict temporal order during proopiomelanocortin biosynthetic proc- essing. A n Zhou, Brian T. Bloomquist, and Richard E. Mains

Insulinomimetic effect on glucose transport by epider-

compatibility complex class I-derived peptide. J a n mal growth factor when combined with a major histo-

Stagsted, SBren Ziebe, Shinobu Satoh, Geoffrey D. Holman, Samuel W. Cushman, and Lennart Olsson

Direct analysis of the binding of the abl Src homology

receptor. Guochang Zhu, Stuart J. Decker, Bruce J . Mayer, 2 domain to the activated epidermal growth factor

and Alan R. Saltiel

Isolation and properties of adenovirus type 2 protein-

dine Rancourt, and Joseph M. Weber ase. Karoly Tihanyi, Martin Bourbonniere, Alain Houde, Clnu-

Differential regulation of adenylylcyclases in vegeta-

Zhang and William J. Snell tive and gametic flagella of chlamydomonas. Yuhua

Topology of P-glycoprotein as determined by epitope mapping of MRK-16 monoclonal antibody. Elias Georges, Takashi Tsuruo, and Victor Ling

A second groEL-like gene, organized in a groESL op- eron is present in the genome of Synechocystis sp. PCC 6803. Csaba Lehel, Dmitry Los, Hajime Wada, Janos Gyor- gyei, Ibolya Horucith, Eszter Kouacs, Norio Murata, and Lkzld Vigh

1805 Binding of DNA quenches tyrosine fluorescence of RecA without energy transfer to DNA bases. Suante Eriksson, Bengt Norden, and Masayuki Takahashi

1811 Role of tyrosine residue 264 of RecA for the binding of cofactor and DNA. Suante Eriksson, Bengt Nordhn, Katsumi Morimatsu, Toshihiro Horii, and Masayuki Takahashi

1817 Removal of stable tyrosine radical D+ affects the struc- ture or redox properties of tyrosine Z in manganese- depleted photosystem I1 particles from Synechocystis 6803. Renee J . Boerner, Kathryn A. Bixby, A n h P. Nguyen, George H. Noren, Richard J . Debus, and Bridgette A. Barry

1824 Cloning and sequencing of a cDNA encoding Saccha- romyces cerevisiae carnitine acetyltransferase. Use of the cDNA in gene disruption studies. Gyula Kispal, Balazs

hamer Tomcsanyi, and Attila Sandor Sumegi, Klaus Dietmeier, Ildiko Bock, Gabriella Gajdos, Ti-

1830 Cold adaptations in Drosophila. Qualitative changes of triacylglycerols with relation to overwintering. Takashi Ohtsu, Chihiro Katagiri, Masahito T. Kimura, and Samuel H. Hori

1835 Cloning and functional expression in yeast of two hu-

channel, the voltage-dependent anion channel. Elizabeth man isoforms of the outer mitochondrial membrane

Adams, Edward R. B. McCabe, John Adelman, Marco Colom- Blachly-Dyson, E . Brygida Zambronicz, Wei Hong Y u , Volker

bini, and Michael Forte

1842 Pteridine biosynthesis in human endothelial cells. Im- pact on nitric oxide-mediated formation of cyclic GMP.

Arno Hausen, Gilbert Reibnegger, Kurt Schmidt, Gunter Weiss, Gabriele Werner-Felmayer, Ernst R. Werner, Dietmar Fuchs,

and Helmut Wachter

1847 The structural motif glycine 190-valine 202 of the fibrinogen y chain interacts with CD1 lb/CD18 integrin (a&, Mac-1) and promotes leukocyte adhesion. Dario C. Altieri, Janet Plescia, and Edward F. Plow

1854 The glucocorticoid receptor and a putative repressor protein coordinately modulate glucocorticoid respon- siveness of the mouse mammary tumor virus promoter in the rat hepatoma cell line M1.19. Hirotoshi Tanaka, Y u Dong,, Jacqueline M*cGuire, S a m Okret, Lorenz Poellinger, Isao Makmo, and Jan-Ake Gustafsson

1860 Polypeptide composition of the a-latrotoxin receptor. High affinity binding protein consists of a family of related high molecular weight polypeptides complexed to a low molecular weight protein. Alexander G. Petrenko, Victoria D. Lazaryeua, Martin Geppert, Tatyana A. Tarasyuk, Carolyn Moomaw, Andrei V . Khokhlatcheu, Yuri A. Ushkar- you, Cliue Slaughter, Igor V. Nasimou, and Thomas C. Sudhof

1868 Translational regulation of mitochondrial differentia- tion in neonatal rat liver. Specific increase in the trans-

drial 8-F1-ATPase mRNA. Ana M. Luis, Jose M. Izquierdo, lational efficiency of the nuclear-encoded mitochon-

Luciana K. Ostronoff, Matilde Salinas, Juan F. Santaren, and Josh M. Cuezua

1876 Control of protein traffic between distinct plasma mem- brane domains. Requirement for a novel 108,000 pro- tein in the fusion of transcytotic vesicles with the apical plasma membrane. Elizabeth Sztul, Maria Colombo, Philip Stahl, and Reba Samanta

1886 Cross-competition for binding of a,-antitrypsin (al AT)-elastase complexes to the serpin-enzyme complex receptor by other serpin-enzyme complexes and by pro- teolytically modified al AT. Gregg Joslin, Art Wittwer,

H. Perlmutter Steve Adams, Douglas M. Tollefsen, Anna August, and Dauid

1894 Cloning of a novel surface antigen from the insect stages of Trypanosoma brucei by expression in COS cells. David G. Jackson, Dean K. Smith, Colin Luo, and John F. Elliott

1901 ATP induces a conformational change of the 90-kDa

Miklos Hollosi, George Jalsouszky, Sandor Holly, C. Ronald heat shock protein (hsp9O). Peter Csermely, Judit Kajtar,

Kahn, Peter Gergely, Jr., Csaba Soti, Katalin Mihaly, and Janos Somogyi

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Expression of the nitric oxide synthase gene in mouse

lar basis for the synergy between interferon-y and macrophages activated for tumor cell killing. Molecu-

lipopolysaccharide. Robert B. Lorsbach, William J. Murphy,

Russell Charles J. Lowenstein, Solomon H. Snyder, and Stephen W.

Coupling of cytosolic protein synthesis and mitochon- drial protein import in yeast. Evidence for cotransla- tional import in vivo. Masaaki Fujiki and Keith Verner

Activating and inhibitory mutations in the regulatory domain of CheB, the methylesterase in bacterial cbemo- taxis. R i c h a ~ C. Stewart

DNA repair by eukaryotic nucleotide excision nuclease. Removal of thymine dimer and psoralen monoadduct by HeLa cell-free extract and of thymine dimer by Xenopus iaevis oocytes. Daniel L. Suobodn, John-Stephen Taylor, John E. Hearst, and Aziz Sancar

Sequence analysis of lens &crystallins suggests in- volvement of calpain in cataract formation. Larry L. David, Thomas R. Shearer, and Marjorie Shih

The motif Tyr-X-X-hydrophobic residue mediates ty- sosomal membrane targeting of lysosome-associated membrane protein 1. Frank G. Guarnieri, Linda M. Arter- burn, Margaret B. Penno, Ying Cha, and J . Thomas August

Binding of the RNA polymerase I transcription com- plex to its promoter can modify positioning of down- stream nucleosomes assembled in vitro. Philippe Georgel,

sal E. uan Holde Borries Demeler, Chris Terpening, Marvin R, Paule, and Ken-

Point mutagenesis of carboxyl-terminal amino acids of cholesteryl ester transfer protein. Opposite faces of an amphipathic helix important for cholesteryl ester transfer or for binding neutralizing antibody. Suke Wang, Xingbo Wang, Liping Deng, Eric Rassart, ROSS W. Milne, and Alan R. Tall

P h ~ h o r y l a t i o n a n d activation of a high molecular weight form of phospholipase A2 by p42 microtubule-

phael A. Nemenoff, Sim Winitz, Nan-Xin Qian, Vicki Van associated protein 2 kinase and protein kinase C. Ra-

Putten, Gary L. Johnson, and Lynn E. Heasley

Character i~t ion of a DNA polymerase from the hy- perthermophile archaea Thermococcus Zitorali8. Vent DNA polymerase, steady state kinetics, thermal stabfl- ity, processivity, strand displacement, and exonuclease

Jack activities. Huimin Kong, Rebecca B. Kucera, and William E.

A 40-kDa epidermal growth factoritransforming growth factor a-binding domain produced by limited proteolysis of the extracellular domain of the epidermal

Irit LAX, Hiroshi Kawasaki, Koichi Suauki, Axel Ullrich, Joseph growth factor receptor. Vaisuke Kohda, Masafumi Odaka,

Schlessinger, and Fuyuhiko Inagaki

A myristoylated pseudosuhstrate peptide, a novel pro-

Bont, John de Widt, Rob M. J. Liskamp, and Hidde L. Ploegh tein kinase C inhibitor. Thomas Eichholtz, Dries B. A. de

Angiotensin I1 regulates parathyroid hormone-related

cells through transcriptional and post-transcriptional protein expression in cultured rat aortic smooth muscle

mechanisms. Carlos J . Pirola, Hai-mei Wang, Amin Kamyar, Siaosing Wu, Hiro Enomoto, Behrooz Sharifi, James S. Forres- ter, Thomas L. Clemens, and James A. Fagin

Enhancement by protein kinase C of prostacydin receptor-mediated activation of adenylate cyclase through a calmodulin/myristoylated alanine-rich C ki- nase substrate (MARCKS) system in IC2 mast cells.

Ohno, and Atsushi Ichikawa Tohru Sawai, Manabu Negishi, Nobuhiro Nishigaki, Tadao

Role of endoplasmic reticular calcium in oligosaccha- ride processing of crl-antitrypsin. Galina Kuznetsou, Mar- garet A. Brostrom, and Charles 0. Brostrom

Proteolytic processing of human amyloid t3 protein pre- cursor in insect cells. Major carboxyl-terminal frag-

Ramabhadran, Samuel E. Gandy, Jorge Ghiso, Andrew J . Czer- ment is identical to its human counterpart. Triprayar V.

nik, David Ferris, Ramaninder Bhasin, Dmitry Goldgaber, Blas Frangione, and Paul Greengard

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Evidence for an extended structure of the T-cell co- receptor CD8a as deduced from the hydrodynamic

Jean Philippe Boursier, Andres Alcover, Frederique Herue, properties of soluble forms of the extracellular region.

Isabelle Laisney, and Oreste Acuto

The D domain of the thyroid hormone receptor a1 specifies positive and negative tran~riptional regula- tion functions. Youngsoak Lee and Vijak Mahdaui

Changes in the structure and catalytic activities of the bovine pituitary multicatalytic proteinase complex fol- lowing dialysis. Bo Yu, Maria E. Pereira, and Sherwin Wilk

Regulation of the cystic fibrosis transmembrane con- ductance regulator C1’ channel by specific protein kinases and protein phosphatases. Herbert A. Berger, Sue M. Trauis, and Michael J . Welsh

Identification of the herpes simplex virus-I protease cleavage sites by direct sequence analysis of autopro- teolytic cleavage products. Carolyn L. Dilanni, Diana A. Drier, Ingrid C. Deckman, Patrick J . McCann III, Fenyong Liu, Bernard Roizman, Richard J . Colonno, and Mcchael G. Cordingley

sophila Ca~/calmodulin-dependent protein kinase I1 Molecular characterization and expression of the Dro-

gene. Identification of four forms of the enzyme generated from a single gene by alternative splicing. Shunji Ohsako, Yasuyoshi Niahida, Haruko Ryo, and Takashi Yamauchi

Aspartyl residue 10 is essential for ATPase activity of rat hsc70. Shim-ping Hung, Ming-Ying Tsai, Ywh-Min Tzou, Wen-guey Wu, and Chung Wang

Degradation of entactin by matrix metalloproteinases. Susceptibility to matrilysin and identification of cleav- age sites. Ulrike I. Sires, Gait L. Griffin, T~~ J. Broekej-

Howard G. Welgus, and Robert M. Senior mann, Robert P. Mecham, Gillian Murphy, Albert E. Chung,

Expression of Drosophila Rrpl protein in Escherichia coli. Enzymatic and physical characterization of the intact protein and a carboxyl-terminally deleted exo- nuclease-deficient mutant. Miriam Sander, Meryl Carter, and Shu-Mei Huang

The rat liver ecto-ATPase is also a canalicular bile acid transport protein. C. Jeffrey Sippel, Frederick J. Suchy, M. Ananthanurayanan, and Vavid H. Perlmutter

Novel 8-base pair sequence (Drosophila DNA replica- tion-related element) and specific binding factor in-

polymerase a and proliferating cell nuclear antigen. volved in the expression of Drosophila genes for DNA

Fumiko Hirose, Masamitsu Yamaguchi, Hiroshi Handa, Yukio Inomata, and Akio ~ f a ~ u ~ g e

Accumulation of 1,2-sn-diradylglycerol with increased membrane-associated protein kinase C may be the

choline-deficient rats. Kerry-Ann da Costa, Elizabeth F. mechanism for spontaneous hepatocarcinogenesis in

Zeiset Cochury, Jan K. Blusztajn, Sanford C. Garner, and Steven H.

Molecular characterization of four pharmacologically distinct a-aminobutyric acid transporters in mouse brain. Qing-Rong Liu, Beatriz Lcipez-Corcuera, Sreekala Man- diyan, Hannah Nelson, and Nathan Nelson

Site-directed mutagenesis of HIV-1 integrase demon- strates differential effects on integrase functions in vitro. Andrew D. Leavitt, Lily Shiue, and Harold E. Varmus

Structure, organization, and chromosomal mapping of the human macrophage scavenger receptor gene. Mit- suru Emi, Hitoshi Asaoka, Akiyo Matsurnoto, Hiroshige Itak- ura, Yukiko Kurihara, Yoichiro Wada, Hiroshi Kanamori,

Lalouel, Tatsuhiko Kodama, and Tsunehiro Mukai Yoshio Yazaki, Ei-ichi Takahashi, Mark Lepert, Jean-Marc

Charged collagen structure mediates the recognition of negatively charged macromolecules by macrophage scavenger receptors. Takefumi Doi, Ken-&hi Higashino, Yukiko Kurihara, Yoichiro Wada, Tohru Miyazak!, Haruki Nakamura, Seiichi Uesugi, Takeshc Imanishi, Yoshtkt Kawabe, Hiroshige Itakura, Yoshco Yazaki, AkLyo Matsumoto, and Tat- suhiko Kodama Recombinant human tumor necrosis factor Q induces calcium oscillation and calcium-activated chloride cur- rent in human neutrophils. The role of calcium/calmod- ulin-dependent protein kinase. Muhammad A. Schurnann, Phyllis Gardner, and Thomas A. Raffm

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Inactivation of a redox-sensitive protein phosphatase during the early events of tumor necrosis factorbnter- leukin-1 signal transduction. Gmeme R. Guy, Jennifer Cairns, Siew Bee Ng, and Y. H. Tan

Direct association of interleukin-6 with a 130-kDa component of the interleukin-6 receptor system. Fran- cesco D’Alessandro, Oscar R. Colamonici, and Richard P. Nordan

Regulation of human tissue factor expression by mRNA turnover. Shawn M . Ahern, Toshiyuki Miyata, and J. Euan Sadler

tion in the topoisomerase I1 cDNA from an etoposide- Molecular cloning and identification of a point muta-

resistant Chinese hamster ovary cell line. Victor T. W. Chon, Shu-wing Ng, J. Paul Eder, and Lowell E. Schnipper

of vaccinia RNA polymerase. Jeremiah Hagler and Stewart Nascent RNA cleavage by purified ternary complexes

Shuman

Guanylyl cyclase C is an N-linked glycoprotein recep-

binding proteins in the intestine. Arie B. Vaandrager, tor that accounts for multiple heat-stable enterotoxin-

Stephanie Schulz, Hugo R. De Jonge, and David L. Garbers

Activation of the human vimentin gene by the Tax human T-cell leukemia virus I. Mechanisms of regula- tion by the NF-KB transcription factor. Ahin Lilienbaum and Denise Paulin

A pathogen-res~nsive gene of parsley encodes tyro- sine decarboxylase. Petra Kawalleck, Harald Ketler, Klaus Hahlbrock, Dierk Scheel, and Imre E. Somssich

Dominant lethal mutations near the 5’ substrate bind- ing site affect RNA polymerase propagation. Valery Sag- itou, Vadim Nikiforov, and Alex Goldfarb

Stimulation of poly(A) tail elongation by the VP39 subunit of the vaccinia virus-encoded poly(A) polym- erase. Paul David Gershon and Bernard Moss

Selection of a mutant cell line based on differential expression of glycosphingolipid, utilizing anti-lactosyl- ceramide antibody and complement. Tsutomu Tsuruoka, Tsutomu Tsuji, Hisao Nojiri, Eric H. Holmes, and Sen-itiroh Hakomori

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GENERAL INFOR~ATION

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Regulatory role of GMs ganglioside in a661 integrin receptor for fibronectin-mediated adhesion of FUA169 cells. Ming~he Zheng, Hang Fang, Tsutomu Tsuruokn, Tsu- tomu Tsuji, Tomikazu Sasaki, and Sen-itiroh Hakomori

Structural and functional changes of lysosomal acid a- ghcosidase during intracellular transport and matu- ration. Ifeleen A. W i s s e ~ r ~ Marinn A. K m s , Moniqw M. P. Hermans, Jos van Beeumen, and Arnold J. J. Reuser

Dissection of the biotinyl subunit of transcarboxylase into regions essential for activity and assembly. B. C. Shenoq: Ganesh R Kunar, and David Samols Hydrodynamic properties and immunological identifi- cation of the sodium- and chloride-coupled glycine transporter. Beatriz L@xz-Corcuera, Rufael Alcantara, Jesus Vazquez, and Carmen Aragbn

HRas-dependent pathways can activate morphological and genetic markers of cardiac muscle cell hypertro- phy. Andrew Thorburn, Jackie Thorburn, Sei-Yu Chen, Scott Powers, Huda E. Shubeita, James R. Feramisco, and Kenneth R. Chien

Mitogen-activated protein kinase stimulation by a ty-

tor. Erica Selua, Dauid L. Raden, and Roger J. Davis rosine kinase-negative epidermal growth factor recep-

c-myb transactivates cdc2 expression via Myb binding sites in the 6’-flanking region of the human cdc2 gene.

~ i c o ~ i d e s , Kenneth E. Lipson, Thorn# A. Marinq and Bruno De-Hui Ku, Sku-Ching Wen, Andrew Engelhard, Niciwlns C.

Calabretta

Glycophorin B and glycophorin E genes arose from the glycophorin A ancestral gene via two duplications dur- ing primate evolution. Ann Rearden, Andrew Magnet, Shin- ichi Kudo, and Minoru Fukuda

ADDITIONS AND CORRECTIONS Characterization of a cDNA encoding the 70-kDa sin-

tion protein A and the role of the protein in DNA gle-stranded DNA-binding subunit of human replica-

replication. Vol. 266 (1991) 12090-12098. Lorne F. Erdile, Wolf-Dietrich Heyer, Richard Kolodner, and Thomas J. Kelly

TvDe I human comDlement C2 deficiencv. A 28-base p i i gene deletion causes skipping of exon 6 during RNA splicing. Vol. 267 (1992) 9347-9353. Charles A. Johnson, Peter Densen, Robert K. Hurford, Jr., Harvey R. Colten, and Rick A. Wetsel

Cumulative author index. Numbers 1 through 3.

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Vol. 268, No. 3 The Journal of Biological Chemistry January 25, 1993

CONTENTS Arranged by Subject Categories

MINIREVIEW 1501 The MARCKS family of cellular protein kinase C sub-

strates. Perry J . Blackshear

CARBOHYDRATES, LIPIDS, AND OTHER NATURAL PRODUCTS

1618 a- and B-xylosides alter glycolipid synthesis in human melanoma and Chinese hamster ovary cells. Hudson H. Freeze, Deepak Sampath, and Ajit Varki

1735 Hydrolysis of short acyl chain inositol lipids by phos- pholipase C-6,. Mario J . Rebecchi, Robert Eberhardt, Tracy Delaney, Shaukat Ali, and Robert Bittman

1830 Cold adaptations in Drosophila. Qualitative changes of triacylglycerols with relation to overwintering. Takashi Ohtsu, Chihiro Katagiri, Masahito T. Kimura, and Samuel H. Hori

2211 Selection of a mutant cell line based on differential expression of glycosphingolipid, utilizing anti-lactosyl- ceramide antibody and complement. Tsutomu Tsuruoka, Tsutomu Tsuji, Hisao Nojiri, Eric H. Holmes, and Sen-itiroh Hakomori

2217 Regulatory role of GW3 ganglioside in cy501 integrin receptor for fibronectin-mediated adhesion of FUA169 cells. Mingzhe Zheng, Hang Fang, Tsutomu Tsuruoka, Tsu- tomu Tsuji, Tomikazu Sasaki, and Sen-itiroh Hakomori

CELL BIOLOGY AND METABOLISM '1513 Disruption of potential sites for N-linked glycosylation

does not impair hormone binding to the lutropinlcho- riogonadotropin receptor if Asn-173 is left intact. Xuebo Liu, Dauid Dauis, and Deborah L. Segaloff

-1521 The biological relevance of the binding of calcium ions by inositol phosphates. Brian M. Luttrell

1580 Growth factors that repress myoblast differentiation

Francesca Cole, Thomas M. Fasy, Sunkara S. Rao, Mary Anne sustain phosphorylation of a specific site on histone H1.

de Peralta, and D. Staue Kohtz

1610 Effects of intracellular amino acid concentrations, cyclic AMP, and dexamethasone on lysosomal proteol- ysis in primary cultures of perinatal rat hepatocytes. Pietjan J . E. Blornmaart, Duco Zonneueld, Alfred J . Meijer, and Wouter H. Lamers

1749 Ca'+-dependent and Ca2+-independent isozymes of pro- tein kinase C mediate exocytosis in antigen-stimulated

tory responses with CaZ+ and purified isozymes in rat basophilic RBL-2H3 cells. Reconstitution of secre-

washed permeabilized cells. Koichiro Ozawa, Zoltan Szal- lasi, Marcelo G. Kazanietz, Peter M. Blumberg, Harald Mis- chak, J . Frederic Mushinski, and Michael A. Beauen

1757 An N-terminal glycosylation signal on cytochrome P450 is restricted to the endoplasmic reticulum in a luminal orientation. Elzbieta Szczesna-Skorupa and Byron Kemper

1763 The prohormone convertases PC1 and PC2 mediate

order during proopiomelanocortin biosynthetic proc- distinct endoproteolytic cleavages in a strict temporal

essing. A n Zhou, Brian T. Bloomguist, and Richard E. Mains

1770 Insulinomimetic effect on glucose transport by epider- mal growth factor when combined with a major histo- compatibility complex class I-derived peptide. Jan Stagsted, S ~ e n Ziebe, Shinobu Satoh, Geoffrey D. Holman, Samuel W. Cushman, and Lennart Olsson

1775 Direct analysis of the binding of the ab1 Src homology 2 domain to the activated epidermal growth factor

and Alan R. Saltiel receptor. Guochang Zhu, Stuart J. Decker, Bruce J . Mayer,

1786 Differential regulation of adenylylcyclases in vegeta-

Zhang and William J . Snell tive and gametic flagella of chlamydomonas. Yuhua

1799 A second groEL-like gene, organized in a groESL op- eron is present in the genome of Synechocystis sp. PCC

gyei, Ibolya Horuath, Eszter Kouacs, Norio Murata, and L&zlo 6803. Csaba Lehel, Dmitry L m , Hajime Wada, Janos Gyor-

Vigh

1842 Pteridine biosynthesis in human endothelial cells. Im- pact on nitric oxide-mediated formation of cyclic GMP. Gabriele Werner-Felmayer, Ernst R. Werner, Dietmar Fuchs, Arno Hausen, Gilbert Reibnegger, Kurt Schmidt, Giinter Weiss, and Helmut Wachter

1847 The structural motif glycine 190-valine 202 of the fibrinogen y chain interacts with CD1 lb/CD18 integrin

C. Altieri, Janet Plescin, and Edward F. Plow ( a d z , Mac-1) and promotes leukocyte adhesion. Dario

1854 The glucocorticoid receptor and a putative repressor protein coordinately modulate glucocorticoid respon- siveness of the mouse mammary tumor virus promoter in the rat hepatoma cell line M1.19. Hirotoshi Tanaka,

Isao Makino, and Jan-Ake Gustafsson Y u Dong, Jacqueline MSGuire, S a m Okret, Lorenz Poellinger,

1868 Translational regulation of mitochondrial differentia- tion in neonatal rat live;,. Specific increase in the trans-

drial B-F1-ATPase mRNA. Ana M. Luis, Jose M. Izguierdo, lational efficiency of the nuclear-encoded mitochon-

Luciana K. Ostronoff, Matilde Salinas, Juan F. Santaren, and Jose M. Cuezua

1876 Control of protein traffic between distinct plasma mem- brane domains. Requirement for a novel 108,000 pro- tein in the fusion of transcytotic vesicles with the apical

Stahl, and Reba Samanta plasma membrane. Elizabeth Sztul, Maria Colombo, Philip

1886 Cross-competition for binding of al-antitrypsin (a1 AT)-elastase complexes to the serpin-enzyme complex receptor by other serpin-enzyme complexes and by pro- teolytically modified al AT. Gregg Joslin, Art Wittwer, Steve Adams, Douglas M. Tollefsen, Anna August, and Dauid H. Perlmutter

1894 Cloning of a novel surface antigen from the insect stages of Trypanosoma brucei by expression in COS cells. Dauid G. Jackson, Dean K. Smith, Colin Luo, and John F. Elliott

1908 Expression of the nitric oxide synthase gene in mouse macrophages activated for tumor cell killing. Molecu- lar basis for the synergy between interferon-y and lipopolysaccharide. Robert B. Lorsbach, William J. Murphy, Charles J. Lowenstein, Solomon H. Snyder, and Stephen W . Russell

1914 Coupling of cytosolic protein synthesis and mitochon- drial protein import in yeast. Evidence for cotransla- tional import in vivo. Masaaki Fujiki and Keith Verner

1941 The motif Tyr-X-X-hydrophobic residue mediates ly- sosomal membrane targeting of lysosome-associated membrane protein 1. Frank G. Guarnieri, Linda M. Arter- burn, Margaret B. Penno, Ying Cha, and J. Thomas August

1960 Phosphorylation and activation of a high molecular weight form of phospholipase A2 by p42 microtubule-

phael A. Nemenoff, Sim Winitz, Nan-Xin Qian, Vicki Van associated protein 2 kinase and protein kinase C. Ra-

Putten, Gary L. Johnson, and Lynn E. Heasley

1982 A myristoylated pseudosubstrate peptide, a novel pro-

Bont, John de Widt, Rob M. J. Liskamp, and Hidde L. Ploegh tein kinase C inhibitor. Thomas Eichholtz, Dries B. A. de

1987 Angiotensin I1 regulates parathyroid hormone-related

cells through transcriptional and post-transcriptional protein expression in cultured rat aortic smooth muscle

Siaoxing Wu, Hiro Enomoto, Behrooz Sharifi, James S. Forres- mechanisms. Carlos J. Pirola, Hai-mei Wang, Amin Kamyar,

ter, Thomas L. Clemens, and James A. Fagin

Indicates a Communication

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Enhancement by protein kinase C of prostacyclin receptor-mediated activation of adenylate cyclase through a calmodulin/myristoylated alanine-rich C ki- nase substrate (MARCKS) system in IC2 mast cells. Tohru Sawai, Manabu Negishi, Nobuhiro Nishigaki, Tadao Ohno, and Atsushi Ichikawa

Role of endoplasmic reticular calcium in oligosaccha-

garet A. Brostrom, and Charles 0. Brostrom ride processing of a,-antitrypsin. Galina Kuznetsou, Mar-

Proteolytic processing of human amyloid j3 protein pre- cursor in insect cells. Major carboxyl-terminal frag- ment is identical to its human counterpart. Triprayar V. Ramabhadran, Samuel E. Gandy, Jorge Ghiso, Andrew J . Czer-

Frangione, and Paul Greengard nik, David Ferris, Ramaninder Bhasin, Dmitry Goldgaber, BEas

sophila Ca2+/calmodulin-dependent protein kinase 11 Molecular characterization and expression of the Dro-

gene. Identification of four forms of the enzyme generated from a single gene by alternative splicing. Shunji Ohsako, Yasuyoshi Nishida, Haruko Ryo, and Takashi Yamauchi

The rat liver ecto-ATPase is also a canalicular bile acid transport protein. C. Jeffrey Sippel, Frederick J. Suchy, M. Ananthanarayanan, and David H. Perlmutter

Accumulation of 1,2-sn-diradylglycerol with increased membrane-associated protein kinase C may be the

choline-deficient rats. Kerry-Ann da Costa, Elizabeth F. mechanism for spontaneous hepatocarcinogenesis in

Zeisel Cochary, Jan K. Blusztajn, Sanford C. Garner, and Steven H.

Recombinant human tumor necrosis factor a induces calcium oscillation and calcium-activated chloride cur-

ulin-dependent protein kinase. Muhammad A. Schumann, rent in human neutrophils. The role of calcium/calmod-

Phyllis Gardner, and Thomas A. Raffin

Inactivation of a redox-sensitive protein phosphatase during the early events of tumor necrosis factor/inter- leukin-1 signal transduction. Graeme R. Guy, Jennifer Cairns, Siew Bee Ng, and Y. H. Tan

component of the interleukin-6 receptor system. Fran- Direct association of interleukin-6 with a 130-kDa

Nordan cesco D'Alessandro, Oscar R. Colamonici, and Richard P.

Guanylyl cyclase C is an N-linked glycoprotein recep-

binding proteins in the intestine. Arie B. Vaandrager, tor that accounts for multiple heat-stable enterotoxin-

Stephanie Schulz, Hugo R. De Jonge, and David L. Garbers

Mitogen-activated protein kinase stimulation by a ty- rosine kinase-negative epidermal growth factor recep- tor. Erica Selua, David L. Raden, and Roger J . Davis

ENZYMOLOGY Evidence for specific base catalysis in N-dealkylation

peroxidase. Differences in rates of deprotonation of reactions catalyzed by cytochrome P450 and chloro-

aminium radicals as an explanation for high kinetic hydrogen isotope effects observed with peroxidases. Osamu Okazaki and F. Peter Guengerich

Chemical modification of bacteriophage T4 deoxynu- cleotide kinase. Evidence of a single catalytic region. George S. Brush and Maurice J . Bessman

tions. Oxidation of iodide, thioanisoles, and phenols at Horseradish peroxidase-catalyzed two-electron oxida-

R. Ortiz de Montellano distinct sites. Robert 2. Harris, Sherri L. Newmyer, and Paul

Cooperative phenomena in binding and activation of

Ahmed Bouhss, Euelyne Krin, Helene Munier, Anne-Marie Bordetella pertussis adenylate cyclase by calmodulin.

Gilles, Antoine Danchin, Philippe Glaser, and Octavian Birzu

Characterization of a synthetic calmodulin-binding peptide derived from Bacillus anthracis adenylate cy- clase. Helene Munier, Francisco J . Blanco, Benidicte Pre- cheur, Eric Diesis, Jose L. Nieto, Constantin T. Craescu, and Octavian Birzu

Purification and characterization of membrane-bound chitin synthase. Sachiko Machida and Michihiko Saito

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1965

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2069

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Mechanism of reaction of fatty acid hydroperoxides with soybean peroxygenase. Elizabeth Blee, Allan L. Wil- con, Lawrence J . Marnett, and Francis Schuber

cated cytoplasmic tail (LHR-ct628) desensitizes to the A luteinizing hormone receptor with a severely trun-

Gudermann, Marie1 Birnbaumer, and Lutz Birnbaumer same degree as the full-length receptor. Xi Zhu, Thomas

Activating and inhibitory mutations in the regulatory domain of CheB, the methylesterase in bacterial chemo- taxis. Richard C. Stewart

Characterization of a DNA polymerase from the hy- perthermophile archaea Thermococcus litoralis. Vent DNA polymerase, steady state kinetics, thermal stabil-

activities. Huimin Kong, Rebecca B. Kucera, and William E. ity, processivity, strand displacement, and exonuclease

Jack

Changes in the structure and catalytic activities of the bovine pituitary multicatalytic proteinase complex fol- lowing dialysis. Bo Yu, Maria E. Pereira, and Sherwin Wilk

Identification of the herpes simplex virus-1 protease cleavage sites by direct sequence analysis of autopro- teolytic cleavage products. Carolyn L. Dilanni, Diana A. Drier, Ingrid C. Deckman, Patrick J . McCann III, Fenyong Liu, Bernard Roizman, Richard J . Colonno, and Michael G. Cordingley

Degradation of entactin by matrix metalloproteinases.

age sites. Ulrike I. Sires, Gail L. Griffin, Thomas J . Broekel- Susceptibility to matrilysin and identification of cleav-

mann, Robert P. Mecham, Gillian Murphy, Albert E. Chung, Howard G. Welgus, and Robert M. Senior

Site-directed mutagenesis of HIV-1 integrase demon-

vitro. Andrew D. Leauitt, Lily Shiue, and Harold E. Varmus strates differential effects on integrase functions in

Molecular cloning and identification of a point muta- tion in the topoisomerase I1 cDNA from an etoposide- resistant Chinese hamster ovary cell line. Victor T . W. Chan, Shu-wing Ng, J . Paul Eder, and Lowell E. Schnipper

MEMBRANES AND BIOENERGETICS Cloning and expression of a mammalian Na+/amino acid

cotransporters. Cheng-Te Kong, Shaw-Fang Yet, and Julia cotransporter with sequence similarity to Na+/glucose

E. Lever

Evidence that energization of the chloroplast ATP syn- thase favors ATP formation at the tight binding cata- lytic site and increases the affinity for ADP at another catalytic site. Jun-Mei Zhou and Paul D. Boyer

Studies on the mechanism of oxidative phosphoryla- tion. Different effects of Fo inhibitors on unisite and multisite ATP hydrolysis by bovine submitochondrial particles. Akemi Matsuno-Yagi and Youssef Hatefi

Slow binding of ATP to noncatalytic nucleotide binding sites which accelerates catalysis is responsible for ap- parent negative cooperativity exhibited by the bovine mitochondrial FI-ATPase. Jean-Michel Jault and William S. Allison

interchain disulfide bridge is the rate-limiting step of Cell penetration of diphtheria toxin. Reduction of the

translocation in the cytosol. Emanuele Papini, Rino Rap- puoli, Marta Murgia, and Cesare Montecucco

Genetic and immunological analyses of the cyanobac- terium Synechocystis sp. PCC 6803 show that the pro-

photosystem I1 centers in thylakoid membranes. Lisbet tein encoded by the psbJ gene regulates the number of

K. Lind, Vipula K. Shukla, Karin J. Nyhus, and Himadri B. Pakrasi

Mutations of the molecular chaperone protein SecB

binding protein. Pamela M . Gannon and Carol A. Kumamoto which alter the interaction between SecB and maltose-

Influenza hemagglutinin-mediated membrane fusion does not involve inverted phase lipid intermediates. Toon Stegmann

porter in Escherichia coli. Elhanan Pinner, Yaniu Kotler, Physiological role of NhaB, a specific Na+/H+ anti-

Etana Padan, and Shimon Schuldiner

Indicates a Communication

X

1792 Topology of P-glycoprotein as determined by epitope mapping of MRK-16 monoclonal antibody. Elias Georges, Takashi Tsuruo, and Victor Ling

1817 Removal of stable tyrosine radical D+ affects the struc-

depleted photosystem I1 particles from Synechocystis ture or redox properties of tyrosine 2 in manganese-

6803. Renee J. Boerner, Kathryn A. Bixby, Anh P. Nguyen, George H. Noren, Richard J , Debus, and Bridgette A. Barry

1835 Cloning and functional expression in yeast of two hu- man isoforms of the outer mitochondrial membrane channel, the voltage-dependent anion channel. Elizabeth

Adams, Edward R. B. McCabe, John Adelman, Marco Colom- Blachly-Dyson, E. Brygida Zambronicz, Wei Hang Yu, Volker

bini, and Michael Forte

2037 Regulation of the cystic fibrosis transmembrane con- ductance regulator C1- channel by specific protein kinases and protein phosphatases. Herbert A. Berger, Sue M. Trauis, and Michael J . Welsh

2106 Molecular characterization of four pharmacologically distinct a-aminobutyric acid transporters in mouse brain. Qing-Rong Liu, Beatriz L6pez-Corcuera, Sreekala Man- diyan, Hannah Nelson, and Nathan Nelson

NUCLEIC ACIDS, PROTEIN SYNTHESIS, AND MOLECULAR GENETICS

‘1505

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Promoter activity of human renin 5’-flanking DNA sequences is activated by the pituitary-specific tran- scription factor Pit-1. Jidong Sun, Carole Oddoux, Amy Lazarus, Matthew T. Gilbert, and Daniel F. Catanzaro

Histamine induces a gene-specific synthesis regulation of secretogranin I1 but not of chromogranin A and B in chromaffin cells in a calcium-dependent manner. Jo-

Fischer-Colbrie hann W. Bauer, Rudolf Kirchmair, Claudia Egger, and Reiner

hydrofolate reductase gene after treatment with ultra- Repair of individual DNA strands in the hamster di-

Rodney S. Nairn, Diane S. Okumoto, Karsten Wassermann, violet light, alkylating agents, and cisplatin. Alfred May,

Tinna Steunsner, Jennifer C. Jones, and Vilhelm A. Bohr

Isolation and properties of adenovirus type 2 protein- ase. Karoly Tihanyi, Martin Bourbonniere, Alain Houde, Clau- dine Rancourt, and Joseph M. Weber

Binding of DNA quenches tyrosine fluorescence of RecA without energy transfer to DNA bases. Suante Eriksson, Bengt Norden, and Masayuki Takahashi

Role of tyrosine residue 264 of RecA for the binding of

Morimatsu, Toshihiro Horii, and Masayuki Takahashi cofactor and DNA. Suante Eriksson, Bengt Norddn, Katsumi

Cloning and sequencing of a cDNA encoding Saecha- romyces cereuisiae carnitine acetyltransferase. Use of the cDNA in gene disruption studies. Gyula Kispal, Balazs Sumegi, Klaus Dietmeier, Ildiko Bock, Gabriella Gajdos, Ti- hamer Tomcsanyi, and Attila Sandor

DNA repair by eukaryotic nucleotide excision nuclease. Removal of thymine dimer and psoralen monoadduct by HeLa cell-free extract and. of thymine dimer by Xenopus laevis oocytes. Daniel L. Suoboda, JohnStephen Taylor, John E. Hearst, and Aziz Sancar

plex to its promoter can modify positioning of down- Binding of the RNA polymerase I transcription com-

Borries Demeler, Chris Terpening, Maruin R. Paule, and Ken- stream nucleosomes assembled in vitro. Philippe Georgel,

sal E. uan Holde

The D domain of the thyroid hormone receptor a1 specifies positive and negative transcriptional regula- tion functions. Youngsook Lee and Vijak Mahdaui

coli. Enzymatic and physical characterization of the Expression of Drosophila Rrpl protein in Escherichia

intact protein and a carboxyl-terminally deleted exo- nuclease-deficient mutant. Miriam Sander, Meryl Carter, and Shu-Mei Huang

Novel 8-base pair sequence (Drosophila DNA replica- tion-related element) and specific binding factor in- volved in the expression of Drosophila genes for DNA polymerase a and proliferating cell nuclear antigen. Fumiko Hirose, Masamitsu Yamagwhi, Hiroshi Handa, Yukio Inomata, and Akio Matsukage

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the human macrophage scavenger receptor gene. Mit- Structure, organization, and chromosomal mapping of

suru Emi, Hitoshi Asaoka, Akiyo Matsumoto, Hiroshige Itak- ura, Yukiko Kurihara, Yoichiro Wada, Hiroshi Kanamori,

Lalouel, Tatsuhiko Kodama, and Tsunehiro Mukai Yoshio Yazaki, Ei-ichi Takahashi, Mark Lepert, Jean-Marc

Charged collagen structure mediates the recognition of negatively charged macromolecules by macrophage scavenger receptors. Takefumi Doi, Ken-ichi Higashino, Yukiko Kurihara, Yoichiro Wada, Tohru Miyazaki, Haruki Nakamura, Seiichi Uesugi, Takeshi Imanbhi, Yoshiki Kawabe,

suhiko Kodama Hiroshige Itakura, Yoshio Yazaki, Akiyo Matsumoto, and Tat-

Regulation of human tissue factor expression by mRNA

Sadler turnover. Shawn M. Ahern, Toshiyuki Miyata, and J . Euan

of vaccinia RNA polymerase. Jeremiah Hagler and Stewart Nascent RNA cleavage by purified ternary complexes

Shuman

Activation of the human vimentin gene by the Tax human T-cell leukemia virus I. Mechanisms of regula-

and Denise Paulin tion by the NF-KB transcription factor. Alain Lilienbaum

A pathogen-responsive gene of parsley encodes tyro-

Hahlbrock, Dierk Scheel, and Imre E. Somssich sine decarboxylase. Petra Kawalleck, Harald Keller, Klaus

Dominant lethal mutations near the 5’ substrate bind- ing site affect RNA polymerase propagation. Valery Sag- itou, Vadim Nikiforou, and Alex Goldfarb

Stimulation of poly(A) tail elongation by the VP39 subunit of the vaccinia virus-encoded poly(A) polym- erase. Paul Dauid Gershon and Bernard Moss

HRas-dependent pathways can activate morphological and genetic markers of cardiac muscle cell hypertro- phy. Andrew Thorburn, Jackie Thorburn, Sei- Yu Chen, Scott Powers, Huda E. Shubeita, James R. Feramisco, and Kenneth R. Chien

c-myb transactivates cdc2 expression via Myb binding sites in the 5”flanking region of the human cdc2 gene. De-Hui Ku, Shau-Ching Wen, Andrew Engelhard, Nicholas C. Nicolaides, Kenneth E. Lipson, Thomas A. Marina, and Bruno Calabretta

Glycophorin B and glycophorin E genes arose from the glycophorin A ancestral gene via two duplications dur- ing primate evolution. Ann Rearden, Andrew Magnet, Shin- ichi Kudo, and Minoru Fukuda

pair gene deletion causes skipping of exon 6 during Type I human complement C2 deficiency. A 28-base

Johnson, Peter Densen, Robert K. Hurford, Jr., Haruey R. RNA splicing. Vol. 267 (1992) 9347-9353. Charles A.

Colten, and Rick A. Wetsel

Characterization of a cDNA encoding the 70-kDa sin- gle-stranded DNA-binding subunit of human replica- tion protein A and the role of the protein in DNA replication. Vol. 266 (1991) 12090-12098. Lome F. Erdile, Wolf-Dietrich Heyer, Richard Kolodner, and Thomas J. Kelly

PROTEIN CHEMISTRY AND STRUCTURE Small heat shock proteins are molecular chaperones. Ursula Jakob, Matthias Gaestel, Katrin Engel, and Johannes Buchner

Conformational, thermodynamic, and stability prop- erties of Manduca sexta apolipophorin 111. Robert 0. Ryan, Kim Oikawa, and Cyril M. Kay

pH-dependent stability and membrane interaction of the pore-forming domain of colicin A. Arturo Muga, Juan M. Gonzalez-Manas, Jeremy H. Lakey, Franc Pattus, and Witold K. Surewicz

The number of amphipathic a-helical segments of apo- lipoproteins A-I, E, and A-IV determines the size and functional properties of their reconstituted lipoprotein particles. Ana Jonas, Armin Steinmetz, and Lisa Churgay

Identification of vicinal thiols of phosphoenolpyruvate carboxykinase (GTP). Cristina T. Lewis, Jerome M. Seyer, Robert G. Cassell, and Gerald M. Carlson

Indicates a Communication

xi

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1901

The Streptococcus sanguis platelet aggregation-asso- ciated protein. Identification and characterization of the minimal platelet-interactive domain. Pamela R. Er- ickson and Mark C. Herzberg

Phycobilins of cryptophycean algae. Novel linkage of dihydrobiliverdin in a phycoerythrin 555 and a phy- cocyanin 645. Dauid E . W e m m r , Gary J . Wedemayer, and Alexander N . Glazer

Uptake of high density lipoprotein cholesterol ester by HepG2 cells involves apolipoprotein E localized on the cell surface. Lorraine Leblond and Yues L. Marcel

Isozyme-specific modules on human aldolase A mole- cule. Isozyme group-specific sequences 1 and 4 are

yohisa Motoki, Yoshihiko Kitajima, and Katsuji Hori required for showing characteristics as aldolase A. Ki-

higher plant and bacteria! photosynthetic reaction cen- Precise location of the Cu(I1)-inhibitory binding site in

ters as probed by light-Induced absorption changes. Inmaculada Yruela, Miguel Aljonso, Iriaki Ortiz de Zarate, Guillermo Montoya, and Rafael Picorel

Cumulative effect of double-site mutations of human epidermal growth factor on receptor binding. Stephen R. Campion, Mary K. Geck, and Salil K. Niyogi

High affinity binding protein consists of a family of Polypeptide composition of the a-latrotoxin receptor.

related high molecular weight polypeptides complexed to a low molecular weight protein. Alexander G. Petrenko,

Carol n Moomaw, Andrei V. Khokhlatcheu, Yuri A. Ushkar- Victoria D. Lazaryeua, Martin Geppert, Tatyana A. Tarasyuk,

you, &iue Slaughter, Igor V. Nasimou, and Thomas C. Sudhof

ATP induces a conformational change of the 90-kDa heat shock protein (hsp9O). Peter Csermely, Judit Kajtar, Miklos Hollosi, George Jalsouszky, Sandor Holly, C. Ronald Kahn, Peter Gergely, Jr., Csaba Soti, Katalm Mihdy, and Janos Somogyi

1937

1955

1976

2013

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Sequence analysis of lens b-crystallins suggests in-

Dauid, Thomas R. Shearer, and Marjorie Shih volvement of calpain in cataract formation. Larry L.

cholesteryl ester transfer protein. Opposite faces of an Point mutagenesis of carboxyl-terminal amino acids of

amphipathic helix important for cholesteryl ester transfer or for binding neutralizing antibody. Suke Wang, Xingbo Wang, Liping Deng, Eric Rassart, Ross W . Mzlne, and Alan R. Tall

A 40-kDa epidermal growth factor/transforming growth factor a-binding domain produced by limited proteolysis of the extracellular domain of the epidermal growth factor receptor. Daisuke Kohda, Masajumi Odaka, Irit Lax, Hiroshi Kawasaki, Koichi Suzuki, Axel Ullrich, Joseph Schlessinger, and Fuyuhiko Inagaki

Evidence for an extended structure of the T-cell co- receptor CDBa as deduced from the hydrodynamic

Jean Philippe Boursier, Andres Alcouer, Frederique Herue, properties of soluble forms of the extracellular region.

Isabelle Laisney, and Oreste Acuto

Aspartyl residue 10 is essential for ATPase activity of rat hsc70. Shiao-ping Huang, Ming-Ying Tsai, Ywh-Min Tzou, Wen-guey Wu, and Chung Wang

glucosidase during intracellular transport and matu- Structural and functional changes of lysosomal acid a-

ration. Heleen A. Wisselaar, Marian A. Kroos, Monique M. P. Hermans, Jos uan Beeumen, and Arnold J . J . Reuser

Dissection of the biotinyl subunit of transcarboxylase into regions essential for activity and assembly. B. C. Shenoy, Ganesh K. Kumar, and Dauid Samols

Hydrodynamic properties and immunological identifi- cation of the sodium- and chloride-coupled glycine transporter. Beatriz Ldpez-Corcuera, Rafael Alcantara, Jeslis Vazquez, and Carmen Aragon

Acuto, Oreste, 2013 Adams, Steve, 1886 Adams, Volker, 1835 Adelman, John, 1835 Ahern, Shawn M., 2154 Alcantara, Rafael, 2239 Alcover, Andres, 2013 Alfonso, Miguel, 1684 Ah, Shaukat, 1735

Altieri, Dario C., 1847 Allison, William S., 1558

Ananthanarayanan, M., 2083 Aragbn, Carmen, 2239

Asaoka, Hitoshi, 2120 Arterburn, Linda M., 1941

August, J. Thomas, 1941 August, Anna, 1886

Barry, Bridgette A., 1817 Birzu, Octavian, 1690, 1695 Bauer, Johann W., 1586 Beaven, Michael A,, 1749

Bessman, Maurice J., 1603 Berger, Herbert A., 2037

Bhasin, Ramaninder, 2009 Birnhaumer, Lutz, 1723

Bittman, Robert, 1735 Birnbaumer, Mariel, 1723

Bixby, Kathryn A,, 1817 Blachly-Dyson, Elizabeth,

Blackshear, Perry J., 1501 Blanco, Francisco J., 1695 BIie, Elizabeth, 1708 Blommaart, Pietjan J. E.,

Bloomquist, Brian T., 1763 Blumberg, Peter M., 1749 Blusztajn, Jan K., 2100 Bock, Ildiko, 1824

1835

1610

Boerner, Renee J., 1817

Bouhss, Ahmed, 1690 Bohr, Vilhelm A., 1650

Bourbonniire, Martin, 1780 Boursier, Jean Philippe, 2013

Broekelmann, Thomas J., Boyer, Paul D., 1531

Brostrom, Charles O., 2001

Brush, George S., 1603 Brostrom, Margaret A,, 2001

Buchner, Johannes, 1517

Cairns, Jennifer, 2141 Calahretta, Bruno, 2255 Campion, Stephen R., 1742 Carlson, Gerald M., 1628 Carter, Meryl, 2075 Cassell, Robert G., 1628 Catanzaro, Daniel F.. 1505

2069

Cha, Ying, 1941 Chan, Victor T. W., 2160 Chen, Sei-Yu, 2244 Chien, Kenneth R., 2244 Chung, Albert E., 2069 Churgay, Lisa, 1596 Clemens, Thomas L., 1987 Cochary, Elizabeth F., 2100 Colamonici, Oscar R., 2149

Colombini, Marco, 1835 Cole, Francesca, 1580

Colombo, Maria, 1876 Colonno, Richard J., 2048

Cordingley, Michael G., 2048 Colten, Harvey R., 2268

Craescu, Constantin T., 1695 Csermely, Piter, 1901

Cushman, Samuel W., 1770 Cuezva, Jose M., 1868

Czernik, Andrew J., 2009

AUTHOR INDEX

da Costa, Kerry-Ann, 2100 D’Alessandro, Francesco,

Danchin, Antoine, 1690 David, Larry L., 1937

Davis, Roger J., 2250 Davis, David, 1513

de Bont, Dries B. A., 1982 Debus, Richard J., 1817

Deckman, Ingrid C., 2048 Decker, Stuart J., 1775

De Jonge, Hugo R., 2174 Delaney, Tracy, 1735 Demeler, Borries, 1947 Deng, Liping, 1955 Densen, Peter, 2268 de Peralta, Mary Anne, 1580 de Widt, John, 1982 Diesis, Eric, 1695 Dietmeier, Klaus, 1824 Dilanni, Carolyn L., 2048

Dong, Yu, 1854 Doi, Takefumi, 2126

Drier, Diana A., 2048 Eberhardt, Robert, 1735

Egger, Claudia, 1586 Eder, J. Paul, 2160

Eichholtz, Thomas, 1982 Elliott, John F., 1894 Emi, Mitsuru, 2120 Engel, Katrin, 1517 Engelhard, Andrew, 2255 Enomoto, Hiro, 1987 Erdile, Lorne F., 2268 Erickson, Pamela R., 1646 Eriksson, Svante, 1805,1811 Fagin, James A,, 1987 Fang, Hang, 2217

2149

Fasy, Thomas M., 1580

Ferris, David, 2009 Feramisco, James R., 2244

Fischer-Colbrie, Reiner, 1586 Forrester, James S., 1987 Forte, Michael, 1835 Frangione, Blas, 2009 Freeze, Hudson H., 1618 Fuchs, Dietmar, 1842 Fujiki, Masaaki, 1914 Fukuda, Minoru, 2260

Gaestel, Matthias, 1517 Gajdos, Gabriella, 1824 Gandy, Samuel E., 2009 Gannon, Pamela M., 1590 Garbers, David L., 2174 Gardner, Phyllis, 2134 Garner, Sanford C., 2100 Geck, Mary K., 1742 Georgel, Philippe, 1947 Georges, Elias, 1792

Gergely, Piter, Jr., 1901 Geppert, Martin, 1860

Gershon, Paul David, 2203 Ghiso, Jorge, 2009

Gilles, Anne-Marie, 1690 Gilbert, Matthew T., 1505

Glaser, Philippe, 1690 Glazer, Alexander N., 1658 Goldfarb, Alex, 2195 Goldgaber, Dmitry, 2009 Gonzalez-Manas, Juan M.,

Greengard, Paul, 2009 Griffin, Gail L., 2069 Guarnieri, Frank G., 1941 Gudermann, Thomas, 1723 Guengerich, F. Peter, 1546 Gustafsson, Jan-Ake, 1854

1553

Guy, Graeme R., 2141 Gyorgyei, Janos, 1799 Hagler, Jeremiah, 2166 Hahlbrock, Klaus, 2189 Hakomori, Sen-itiroh, 2211,

Handa, Hiroshi, 2092 Harris, Robert Z., 1637 Hatefi, Youssef, 1539 Hausen, Arno, 1842 Hearst, John E., 1931 Heasley, Lynn E., 1960 Hermans, Monique M. P.,

Herve, Frederique, 2013 Herzberg, Mark C., 1646 Heyer, Wolf-Dietrich, 2268 Higashino, Ken-ichi, 2126 Hirose, Fumiko, 2092 Hollosi, Miklos, 1901 Holly, Sandor, 1901

Holmes, Eric H., 2211 Holman, Geoffrey D., 1770

Hori, Katsuji, 1677 Hori, Samuel H., 1830

Horvath, Ibolya, 1799 Horii, Toshihiro, 1811

Houde, Alain, 1780 Huang, Shiao-ping, 2063 Huang, Shu-Mei, 2075 Hurford, Robert K., Jr., 2268

Imanishi, Takeshi, 2126 Ichikawa, Atsushi, 1995

Inagaki, Fuyuhiko, 1976 Inomata, Yukio, 2092 Itakura, Hiroshige, 2120,

Izquierdo, Josi M., 1868

2217

2223

2126