TianyiJune 2012

NMR analysis protocol

NMR is used to determine the concentration of methylacrylate groups conjugated on the biochemical cues and this allows us to calculate percent of methylacrylation for the amount of methylacrylation catalyst added

Steps:

1. Dissolve 2% polymer (20mg/ml) in D20. Always cap bottle when not in use to prevent water vapor from spoiling the D20.

2. Aliquot out a known volume of polymer solution and add an appropriate amount of standard such that the total concentration of the standard is 10mM.

3. Mix well4. Perform NMR (Leo or XM has the qualification)5. Use MestReNova to analyse the area under the curve to determine the concentration of

methylacrylate groups in the polymer