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Threshold Cycles per reaction (Ct) Fluorescence Negative Positive NGenePlex™ nCoV qRT-PCR Kit Certification CE-IVD Quantity 96 tests Turnaround Time 1.2 hours Target Genes RdRp and N genes on SARS-CoV-2 Specimen Types human nasopharyngeal (NP) swab, oropharyngeal (OP) swab, anterior nasal swab or sputum Compatible Instruments CFX96™ Real-Time PCR Detection System (Bio-Rad) ABI 7500 Real-Time PCR System (Applied Biosystems) QuantStudio™ 3 Real-Time PCR Instrument (Applied Biosystems) LightCycler® 480 System (Roche) Fluorimeter Channel FAM HEX/VIC Cal Red 610/Texas Red NGenePlex ™ nCoVqRT-PCRKit 2X One-step Reaction Mix One-step Enzyme Mix Probe Mix Viral RNA Extraction Kit

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Page 1: NGenePlex nCoVqRT-PCR Kit

Threshold

Cycles per reaction (Ct)

Fluo

resc

ence

Negative

Positive

NGenePlex™ nCoV qRT-PCR Kit

Certification CE-IVD

Quantity 96 tests

Turnaround Time 1.2 hours

Target Genes RdRp and N genes on SARS-CoV-2

Specimen Types human nasopharyngeal (NP) swab, oropharyngeal (OP) swab,anterior nasal swab or sputum

Compatible Instruments

CFX96™ Real-Time PCR Detection System (Bio-Rad)ABI 7500 Real-Time PCR System (Applied Biosystems)

QuantStudio™ 3 Real-Time PCR Instrument (Applied Biosystems)LightCycler® 480 System (Roche)

Fluorimeter ChannelFAM

HEX/VICCal Red 610/Texas Red

NGenePlex™ nCoVqRT-PCR Kit2X One-step Reaction Mix

One-step Enzyme MixProbe Mix

Viral RNA Extraction Kit

Page 2: NGenePlex nCoVqRT-PCR Kit

Products Cat. No. Quantity Storage

NGenePlexTM nCoV qRT-PCR Kit NGB561V-096 96 -20℃

MVPrepTM Viral RNA/DNA Kit plus RP-80100 (04ZZZ00-0005)

100 RT

PathogenSpecies

NGenePlex™nCoV qRT-PCR Kit

Company A / Product B

RdRp N RdRp N

Influenza A virus (H1N1) - - - +

Adenovirus - - - +Human Coronavirus

OC43 - - + -

RdRp

N

RdRp

N

Clinical Results

Positive Negative

NGenePlex™nCoV qRT-PCR kit

Positive 60 0

Negative 0 60

Gene Min. copies

RdRp 20 copies/reaction

N 20 copies/reaction

Pathogen Species

Influenza B virus Mumps virus

Acinetobacter baumannii genomic DNA Mycobacterium avium genomic DNA

Adenovirus Mycobacterium gordonae genomic DNA

Betacoronavirus 1, Strain OC43 Mycobacterium kansasii genomic DNA

Bordetella pertussis genomic DNA Mycobacterium tuberculosis genomic DNA

Haemophilus haemolyticus genomic DNA Pseudomonas aeruginosa genomic DNA

Haemophilus influenzae genomic DNA Respiratory Syncytial virus

Haemophilus parainfluenzae genomic DNA Respiratory Syncytial virus

Herpes Simplex virus Rhinovirus

Human coronavirus 229E SARS-CoV-1

Human coronavirus HKU1 Serratia marcescens genomic DNA

human Coronavirus NL63 Staphylococcus aureus genomic DNA

Influenza A virus (H1N1) Stenotrophomonas maltophilia genomic DNA

Influenza A virus (H3N2) Streptococcus mitis genomic DNA

Klebsiella pneumoniae genomic DNA Streptococcus pneumoniae genomic DNA

Legionella pneumophila genomic DNA Streptococcus pyogenes genomic DNA

MERS Yersinia enterocolitica genomic DNA


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