ndri-pg syllabusdairy processing group
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syllabus for dairy processingTRANSCRIPT
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DAIRY TECH�OLOGY
A.I CORE COURSES
DT-511 Dairy Processing -I 2 + 1
Use of bioprotective factors for preservation of raw milk: effects on physico-chemical, microbial and
nutritional properties of milk and milk products, present status of preservation of raw milk by chemical
preservatives; thermal processing for preservation; methods of determining lethality of thermal processing,
UHT processed milk products, their properties and prospects, types of UHT plants, aseptic fillers, heat
stability and deposit formation aspects, effect on milk quality; techno- economic considerations; principles
and equipment for bactotherm process and its applications in dairy industry; retort processing; dehydration:
advances in drying of milk and milk products; freeze dehydration: physico-chemical changes. during freeze
drying and industrial developments; water activity; sorption behaviour of foods, energy of binding water,
control of water activity of different milk products in relation to their chemical; microbiological and
textural properties; hurdle technology and its application in development of shelf-stable and intermediate-
moisture foods; current trends in cleaning and sanitization of dairy equipment: biological; detergents;
ultrasonic techniques in cleaning; biodetergents, development of sanitizers- heat; chemical; radiation. Food
contaminants: their incidence and implications, corrective measures.
Practical
LP system for extension of keeping quality raw milk, determination of pH; HCT profile of milk systems,
measurement of thiocynate in milk system; determination of water activity and sorption isotherms of milk
products; determination of thermal load during retort processing of milk and milk products; heat
classification of milk powders; functional properties of powders: porosity, interstitial air content, occluded
air content, flowability; determination of degree of browning-chemical/physical methods; freeze drying of
milk/milk products, and heat sensitive products.
Suggested Readings
1. Burton, H. 1998 Ultra-high temperature Processing of Milk and Milk Products, Elsevier Applied
Sciences Ltd., England.
2. Fellow; P. 1988 Food Processing Technology, Elliss Horwood Ltd. Chichester, UK.
3. Troller, J.A. & H.B. Christian. 1978. Water Activity and Food, Food Sci. & Technology, A series of
Monograph Academic Press, London.
4. Gould, G. W. 1995. New Methods of Food Preservation. B1ackie Academic & Professional Pub.
London.
5. IDF Bulletin, 1981. New Monograph on UHT Milk; Document, 133.
DT-521 Dairy Processing –II 2 + 1
Membrane processing and related techniques: classification and characteristics of filtration processes, types
of membranes commercially available, modeling of ultrafiltration processes, mass transfer model,
resistance model, factors affecting flux, during ultrafiltration and reverse osmosis of milk, sweet and sour
whey, ultrafiltration hardware, design of ultrafiitration plants, membrane flouling-problem and treatment,
energy requirements for processing of milk and whey, cleaning and sanitization of different types of
membranes, application of ultrafiltration, reverse osmosis; nanofiltration and microfiltration in the dairy
industry; demineralisation process; importance of demineralisation, different processes available. for
demineralisation; developments in the manufacture and utilization of food and and pharmaceutical grade
lactose from UF permeate; preparation of special foods lik£ low lactose powder; dairy whiteners using UF
retentate, whey protein concentrates, casein and coprecipitates; properties of milk proteins: physical, rheb-
logical and functional properties of whey protein casein; co-precipitates; UF retentate and their
modifications; Evaluation of functional properties.
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Practical
Study of the effect of types of milk, temperature of milk and trans-membrane pressure on the permeate flux
during ultrafiltration process; determination of rennet coagulation properties of milk; performance of
ultrafiltration membrane with respect to permeate flux and volume concentration ratio during processing of
acid and sweet whey; study the effect of types of milk, temperature and applied pressure on the permeate
flux during the reverse osmosis process; nanofiltration of milk, whey and permeate; microfiltration of skim
milk and whey; manufacture of lactose from UF permeate; preparation of whey protein concentrate and its
utilization in dairy products; measurement of different functional properties of casein and whey protein
whipping ability; water binding; emulsification properties; gelling; viscosity and solubility.
Suggested Readings
Renner, E. and Abd EL-Salam, M.H. (1991) Application of ultrafiltration in the Dairy Industry, Elsevier
Applied Sciences, London.
Cheryan, Munir (1998) Ultrafiltration and Microbiltration Handbook, Technomic Publishing House.
Sienkiewizc, T. and RTiedel, c.L. (1990) Whey and Whey Utilization, Verlag Th. Mann Gelsenkirchen-
Buer, Germany.
Zadow, J.G. (1994) Whey and lactose processing, Elsevier Applied Science, London, IDF (1996).
Advances in Membrane Technology for better dairy products, Bull No: JI1.
DT-512 Food Processing 2 + 1
Status of food processing industry in India and abroad; biochemical and physical changes fruits and
vegetables after harvest; use of low temperature; considerations relating to storage of food at chilling
temperature and freezing of fruits and vegetables; use of heat; application of heat energy to foods, concept
of drying rate of fruits and vegetables, industrial drying processes of foods; drying of certain fruits and
vegetables; canning: determination of thermal processes to ensure commercial sterility of foods in cans,
fermentation technology: developments in fermented and pickled vegetables and manufacturing process for
beer and wine; controlled and modified atmospheric storage of fruits and vegetables; enzymatic and non-
enzymatic browning reactions in food and its control; fresh, cured meat and poultry and utilization of other
ingredients in meat products; bakery products; Manufacturing processes for bakery products such as
breads; biscuits; pizza bases, etc; utilization and importance of dairy ingredients in bakery products; use of
egg/egg products in bakery products; advances in milling of cereal grains; processing of mushrooms and its
utilization; nutritional changes during food processing, technologies of fabricated and formulated foods and
their nutritional aspects and extrusion cooking of foods.
Practical
Determination of browning and colour measurement in foods; measurement of Vitamin C content in fruit
juices, preparation pickled vegetables, fruit jams, and bakery products; soups and gravies; their chemical
analysis, experimentation on ripening of fruits with ethylene.
Suggested Readings
Fennema, C.R. (1975) Principles of food science Part-II; Physical principles of food preservation, Marcel
Dekker, Inc, New York.
Potter, N.N. (1987) Food Science; (IV Edition) CBS Publishers and Distributors, Delhi
110032
Norman, W. and Desrosier, IN. (1987) The Technology of Food Preservation (IVth Edition) CBS
Publishers & Distributors; Delhi 110 032
Samuel A. Matz (1996) Bakery Technology and Engineering, (1/1 Edition).
DT -522 Dairy and Food Packaging 2
Status of current packaging; critical review of the existing knowledge in packaging of products; adhesives,
graphics and labeling used in food packaging; protective packaging of foods; packaging of food products
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sensitive to oxygen, light, moisture; special problems in canned foods; packaging of convenience foods;
fruits and vegetables; packaging requirements of fresh fruits and vegetables; packaging of fruit juices; fats
and oils: criteria for selection of proper packaging based on the shelf life desired; packaging of spices;
packaging of meat and poultry: packaging and transportation of fish and other sea-foods; new food
packaging processes; retort pouch technology, microwave packages, bio-degradable packages, edible
packages; industrial packaging: unitizing, palletizing, containenzmg, stacking and materials handling;
distribution systems for packaged foods including prevention of shock damage to articles during transpor-
tation; safety aspects of packaging materials; sources of toxic materials and migration of toxins into food
materials.
Practical
Determination of thickness, GSM, grease resistance, bursting strength, tearing resistance, WVTR, puncture
resistance and edge crush resistance of packaging materials; estimation of shelf life of leafy vegetables and
seasonal fresh. fruits like apples; litchi; and oranges; packaging of turmeric powder and ground red chilli
powder, testing of packaging materials for quality assurance; vacuum packaging of dairy products;
packaging of food products using retort pouch technology; packaging of food products employing
microwave technique.
Suggested Readings
Malhlouthi, M. 1994 Food Packaging and Preservation, Blackie Academic & Professional, Glasgow.
Carol, F; Steinhart, M., Ellin Doyle; Barbara A. and Cochrane, 1995 Food Safety, Marcel Dekker
Inc., Madison, Wisconsin.
Frank A., Paine Heather, Y. Paine 1983 A Handbook of Food Packaging, Leonard Hill, Glasgow.
Sacharow, S. and Griffin, R.C. 1970 Food packaging. The A VI Publication, Westport, CT. USA.
A.2 OPTIO�AL COURSES
DT-513 Rheology of Dairy & Food Products 2+1
Introduction to rheology of foods, physical consideration in study of foods; non-Newtonian fluids;
thixotropy; rheological characterization of foods in terms of stress-strain relationship, creep compliance
and stress relaxation; mechanical models for visco-elastic foods: Maxwell, Kelvin, Burgers and
generalised models and their application; comparative assessments of different types of viscometers;
dynamic measurement of rheology; large deformations and failure in foods: instrumental measurement;
rheological and textural properties of selected dairy products; effect of processing and additives (stabilisers
and emulsifiers) on food product rheology.
Practical
Flow behaviour of fluid dairy products; thixotropy in ice-cream mix; force deformation study of selected
dairy products using an Instron testing machine; effect of test conditions on the texture profile parameters
of cheese; stress relaxation studies in solid foods;. Use of cone penetrometer and extruder for measurement
of butter texture; butter texture evaluation using an extrusion viscometer; rheological evaluation of solid
products using Hoppler consistometer;
Suggested Readings
Sherman, P. (1970) Industrial Rheology; Academic Press; London
Lecture Compendium, CAS/DT Short Course, Aug. 22- September 13(1996) Sensory Evaluation &
Rheology of Milk and milk products, Dairy Technology Division, NDRl, Kamal.
De Mann, J.M., Voisey, P.W., Rasper, V.F., and Stanley, D.W. (1976) Rheology and texture in food
quality, A VI Pub. Co.
DT-514 Dairy Process Biotechnology 3 + 1
Development and impact of biotechnology on food and dairy industry; microbial rennet and recombinant
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chymosin, characteristics and application in cheese making; exogenous free and microencapsulated
enzymes, immobilised enzymes-their application in accelerated ripening of cheese; enzymatically modified
cheeses (EMC) their utilization in various food formulations; technological requirements of modified
micro-organisms for production of cheese and fermented milk products; technological innovations in the
development of functional dairy foods with improved nutritional therapeutic and probiotic attributes;
physiologically active bio-peptidfes/ nutraceuticals; protein hydrolysates-their physico-chemical,
therapeutic properties, production and application in food formulations; production of bio-yoghurt,
probiotic cheese and fermented Illilks; bifidus factors in infant food formulations; microbial
polysaccharides their properties and applications in foods, production of alcoholic beverages and industrial
products from starch; whey and other by-products; bio-sweeteners-types properties and their applications
in dairy and food industry; biopreservatives-characteristics and their application in enhancing the shelf life
of dairy and food products.
Practical
Effect of exogenous enzymes on hydrolysis of protein and fat in culture containing milk systems; to study
the various factors affecting the coagulation of milk by microbial rennets. Manufacture and evaluation of
probiotic cheese and fermented milks; determination of glycolysis, proteolysis and lipolysis in cheese and
fermented milk; enzymatic process for manufacture of low lactose milk whey products.; preparation of
casein hydrolysis; visit to a bioprocessing unit.
Suggested Readings
Alan Wiseman (1988) Principles of Biotechnology, Surrey University Press, New York.
Jack G; Chirikjian (1995) Biotechnology-Theory and Techniques, Jones and Bartlett Publishers; London.
Byong H. Lee (1996) Fundamentals of Food Biotechnology, VCH Publishers, NY. Israel
Goldberg (1994) Functional Foods, Chapman & Hall, New York.
Roger, A. (1989) Food Biotechnology, Elsevier Applied Sci. Pub., UK.
DT-515 Traditional and Value Added Products 2 + 1
Present status of traditional dairy products; globalization of traditional dairy products; plans and policies of
the Government and developmental agencies; process schedule of heat-desiccated, coagulated and
fermented traditional dairy products; process improvement in production of milk sweets; and new products
based on fruits, vegetables and cereals; application of membrane technology; microwave heating for
industrial production of traditional dairy products; advances in industrial production of ghee, flavour and
texture simulation; .techno-economic aspects for establishing commercial units for traditional products:
convenience traditional dairy products; use of natural and permitted synthetic preservatives and new
packaging systems
Practical
Microwave heating of traditional milk delicacies shelf life extension; application of membrane technology
for improving the quality of traditional products from cowlbuffalo milk; preparation of feasibility report
for establishing commercial units for traditional products,
Suggested Readings
Lecture Compendium (1998) Dairy Technology Division, NDRI, Advances in Traditional Dairy Products.
Achaya, K. T. and Rangappa, K.S. (1973) Indian Dairy Products.
Gould, G. W. (1995) New Methods of Food Preservation, Blackie Academic & Professional Pub. London.
DT-516 �ew Product Development 2 + 0
World market and new food products; new product development-criteria; fundamental aspects; role of
sensory analysis; various categories and type of new dairy products; food constituents and functionality;
interdependence of dairy and other food ingredients; use of milk components in formulated foods;
processing alteration and use of milk and milk products for food products development; recent
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developments in food ingredients/additives flavourings, colourings, emulsifiers stabilizer and sweeteners.
interactions with food/milk constituents; nutritional implications; safety aspects; biocatalysts and
biopreservaties in formulating a new/better dairy products; Bio-flavours and flavour enhances: food safety;
marketing strategy and consumer response; economic analysis and costing; recent advances in different
categories and type of dairy product; reduced-additives food and food from supplement-fed animals
response surface methodology.
Suggested Readings
Baker, R.C., Hahn, P.W. and Robbins, K.R. (1994) Fundamentals of New Food Product Development,
Elsevier; New York.
Marjorie, P.P. and Campbell, A.M. (1990) Experimental Food Science, Academic Press, New York.
Khan Riaz Blackie (1993) Low Calories Food and Food Ingredients, Academic and Professional, New
York.
Smit, 1. (1993) Technology of Reduced-additive foods, Blackie Academic and Professional, New York.
Steinhart, C.E., Doyle, M.E., Cochrane, B.A. (1995) Food Safety, Marcel Dekker Inc. New York.
DT-523 Alternative Processes for Dairy & Food Industry 2 + 0
Irradiation: sources and properties of ionizing radiation; mechanism of interaction with microorganisms
and food components microbial inactivation in dairy and food products, chemical effects, packaging,
industrial irradiation systems, benefits and limitations; safety aspects, national and international regulation,
high frequency heating: principles of dielectric heating and factors affecting it, design and working of
microwave oven, continuous microwave heating units, applications in dairy and food processing,
microwavable packaging safety aspects of microwaves, merits and demerits of dieletric heating, infra-red
heating: interaction of infra-red (IR) radiation with penetration properties, equipment; dairy and food
application, advantages and disadvantages of IR heating; Ohmic heating: principle of electric resistance
heating, design of an ohmic heater, operational variables, power consideration, factors affecting heating
efficiency, merits and limitations, food application and future scope; ultrasonic treatment of food:
mechanism of ultrasound induced cell damage,. generation of ultrasound equipment, design of power
ultrasonic system, types of ultrasonic reactors, application of power ultrasound in food processing, effects
on food constituents, ultrasound in consideration with other process alternatives-thermosonication,
advantages and future prospects; High hydrostatic pressure processing: principle of microbial inactivation
barotolerance of microorganisms, effect on food constituents, equipment dairy and food application, merit
and demerits; pulsed electric field processing: description/ mechanism and factors affecting microbial
inactivation effects on food components, present status and future scope for food applications.
Suggested Reading
Gould, G.W. (Ed) (1995) New Methods of Food Preservation. Blackie, London.
Gaonkar, K.G. (Ed) (1995) Food processing: Recent Developments, Elsevier Applied Science, London.
Lecture Compendium Advances in Preservation of Dairy and Food Products (2001) Centre of Advance
Studies in Dairy Technology, Aug. 13- Sept 12, 2001, NDRI, Kamal
Povey, M.J.W. & Mason, T.J (Eds) (1998) Ultrasound in Food Processing, Blackie, London.
Thome, S. (1991) Food Irradiation. Elsevier Applied Science, London.
DT-524 Technology of Functional Foods 2 + 1
Food nutrition and health, Nutritional status and dietary requirement of different target groups and
deficiency diseases. Infant nutrition and dietary Formulations for meeting normal and special needs. I
Special food formulations for the lactose intolerant and diabetics. Therapeutic diets for patients suffering
from acute gastritis, flatulence, peptic ulcers, jaundice, viral hepatitis etc. Low fat, low energy and
slimming foods for the obese. Low cholesterol and low sodium foods. Geriatric and probiotic foods and
nutritional management of the elderly. Food formulations for the sports persons and growing children.
Special dietary foods: Infant foods, Weaning foods, High energy foods, Low residue diets.
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Practical
Production of various formulated foods such as low and high energy foods, high fibre content foods,
probiotic foods, etc. Measurement of dietary fibre contents and cholesteroL
Suggested Readings
Arnold, E. Bender. 1973. Nutrition and Dietetic foods. Chemical Publishing Co., Inc, New York.
Marie V. Krause and L. Kathleen Mahan 1979. Food, Nutrition and Diet Therapy. W.B. Saunders
Company.
J.M. Jussawalla, 1979. Natural Dietetics, Vikas Publishing House Pvt. Ltd.,
Virginia Aronson, 1989. The Dietetic Technician. Effective Nutrition Counseling (2nd Edn) VAN Nostrand
Reinhold, New York.
DT-525 Technology of Food Emulsions, Foams and Gels 2 + 1
Food dispersion, their characteristics and factors affecting food dispersion; food emulsions; emulsifiers and
their functions in foods, emulsions formation and stability, polymers and surfactants; milk foams and their
applications, structure of foams, egg foams and uses, foam formation and their stability; theory of gel
formation, pectic substances and jellies, fruit pectin gels; milk jellies; Structure of dairy foods with
reference to emulsions, foams and gels; physical structure of fat rich, concentrated, fermented, coagulated
and dried products;
Practical
Studies on preparative techniques for structural aspects of dairy foods; Light microscopy of dairy and food
products, scanning electron microscopy of dairy foods; demonstration studies of stability of various food
emulsion systems and foams; determination of gel strength.
Suggested Readings
Blanshard, J/M.V. & Lillford; P. 1987 Food Structure and Behavi Academic Press, London.
Haseuhuetti, G.L. 1977 Food Emulsifiers and their Application, Chapman &Hall, London.
Srinivas, D. and Alain, P. 1977 Food Proteins and their Applications, Marcel Dekker NY.
Ph.D. Core Courses
DT-611 Advances in Lipid Technology 3 + 0
Sources, classification and utilization of commercial edible fats and oils- Current trends. Nutritional
aspects of fats and oils in diets .. Innovations in the production and processing of oils and fats from
different sources, e.g. animal, plant, marine and microbial for utilization in the dairyl food products.
Technology of cooking oils, salad oils and oil based dressings. Frying process and systems, changes in fats
and oils during frying, snack food products and processing systems. Bakery and confectionery fats;
production of modified fats and oils for use in bakery and confectionery products, shortenings and spreads.
Fat replacers, technological developments in low calories spreads and other fat based products. Advances
in technologies for production of plasticisers, emulsifiers and protective coatings.
Suggested Readings
R.J. Hamilton 1995 Developments in oils and fats. Blackie Academic & Professional, Madras.
Kamal, B.S. and Y. Kakuda 1994 Technological Advances in improved and alternative sources of lipids.
Blackie Academic & Professional, Madras.
Y.H. HVI. A. 1996 Wiley Bailey's Industrial oil and fat products. Vol. 1 to 4. Interscience Publication,
John Wiley & Sons, Inc, New York.
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Cambie, R.C. 1989 Fats for the future. International Union of Pure and Applied Chemistry. Pub. by Ellis
Horwood Ltd., Chichester.
Riaz Khan. 1993 Low calories foods and food ingredients. Blackie Academic and Professional, Madras.
Hoffman, G. 1989 Chemistry & Technology of edible oils and fats and their high fat products. Food
Science & Technology-A series of monographs. Academic Press, London.
Hamilton R.J. and A. Bhati. 1987 Recent Advances in Chemistry and Technology of Fats and oils Elsevier
Applied Science, London.
DT-612 Advances in Protein Technology 3 + 0
Denaturation of proteins: Effect of processing parameters on denaturation. Effect of denaturation on the
physico-chemical and biological properties of proteins in food systems. Protein interations with fuod
consituents. Characteristics of proteins from plant, animal and microbial origins. Chemcial behaviour of
protein in food system: protein-protein interactions. Protein-lipid interactions, protein-polysaccharide
interactions, and protein-ion interactions. Significance of protein interactions: formation and stabilization
of casein micelle, stability of concentrated milk products, and role of protein in food structure. Protein
Nutrition: Recent concepts in protein nutrition in man: Enzyme development and protein digestion. Effect
of processing on nutritive value of proteins. Mass and institutional feeding programmes: evaluation of
amino acid fortification of foods, and concepts in protein supplementation and complementation. Recent
Technologies: Augmentation of world resources for protein foods: protein from plants, protein from
animals, protein from microorganisms and plasteins: synthetic proteins. Textured vegetable proteins and
spun fibre technology: Extrusion cooking- selection of ingredients and formulation, control of operational
parameters, microstructure of extrusion cooked foods. Protein isolates/concentrates from single cell
organisms.
Suggested Readings
Fennema, O.R. 1985 Food Chemistry Developments in Dairy Chemistry Vol IV by P.F.Fox (1989)
Walstra, P. and R. Jenness 1984 Dairy Chemistry and Physics IDF Bulletin No.238
Macrac R., R.K. Robinson and M.J. Sadler 1993 IDF Special Issue 9303 Encyclopaedia of Food Sci. Fd.
Tech. and Nutrition Vol. 1-7 (1993).
DT-621 Product Process Monitoring 3 + 0
The concept of product-process monitoring in dairy and food industry. Food Structure Characteristics: (a)
Fat Related Aspects. Application of Thermal Analysis (DTA and DSC vis-a-vis dilatometry) and Pulse
Nuclear Magnetic Resonance (PNMR) spectroscopy in determination of solid-fat content (SFC) of butter in
relation to various processing and storage aspects. (b) Elucidation of crystal characteristics of milk fat in
ghee and other fat-rich products by means of X-ray crystallography and polarising light microscopy with
reference to the impact of cooling and storage/handling conditions on the crystal nature and product
texture. Protein Related Aspects: (a) Influence of heat processing and freezing treatments on protein
denaturation and other conformational as well as aggregation-disaggregation phenomena as monitored
through spectropolarimetry, circular dichronism and differential scanning calorimetry. (b) Process-induced
changes in sub-microscopic particulate properties of milk products as revealed through electron
microscopy with particular emphasis on structure-texture relationship, protein dehydration and re-hydration
(reconstitution) and particle agglomeration (instantization) in milk powders. Flavour Implications of Food
Processing: Aroma analysis employing head-space sampling, extraction, concentration, separation and
identification techniques (GC-MS, random MS and ESR, NMR, IR and Raman Spectroscopy in relation to:
Formation of flavour compounds in milk and milk products during heat processing (including UHT
processing, caramelization and extrusion cooking), fermentation and ripening (cultured. products and
cheese flavour) and storage (Maillard browning). Aroma losses/retention during the drying process (in rela-
tion to milk powder, cheese powder and dry cultured products). Industrial processes for extraction of
desirable and undesirable volatile components from fresh and/or stored products by supercritical fluid
(SCF) technique. Colour Characteristics: Colour and appearance (gloss and translucence) monitoring
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through visual colorimeter, tristimulus colorimeters and reflectance spectrophotometer, CIE, Hunter and
Munsel systems for three dimensional expression of colour. Objective Assessment of Subjective Food
Product Characteristics -Pitfalls and Promises Components Quantification: (3) Microwave moisture
analysis and process control. (b) Application of near infra-red (NIR) reflectance analysis for determination
of proximate principles of cheese and milk powder. (c) Automated analysis of milk and milk products.
Suggested Readings
Peleg, M. and Bagley, E.B. 1983. Physical properties offoods. A VI Publ. Co., Inc, Westport, USA.
Mann, C.M.D. & Jones, A.A. (I 994) Shelf-life evaluation of foods. Blackie Academic & Professional
Pub., London.
Acree, T. E. and Teranishi, R. (1993). Flavour Science: Sensible Principles and Techniques. Amer. Chern.
Soc., Washington, D.C., USA.
Barlett, P.N. et al. 1997. Electronic noses and their application in the food industry.
Schartel, BJ. and Firstenberg, Eden, R. (1988) Biosensors in the Food Industry: Present and Future, 1.
Food Protect. 51 (10) 811-820.
Davenel, A. 1996. On-line control and problems with sensors in quality control for foods and Agricultural
products. J.L. Mutton (Ed.) VCH Pub., London
B. SUPPORTI�G COURSES
DCRT-624 Research Techniques 3 + 1
Electrophoresis: principle and types, isoelectricfocussing; chromatography: column, TLC, GLC, HPLC,
gel-permeation, ion-exchange, affinity, chromatofocussing; spectrophotometry:UV, visible, IR and flame
photometry; potentiometry: principle, various electrodes; electrometric measurements of pH, buffers;
radiotracer technique: nuclear transformation, nuclear decay, measurement of radioactivity and safety
precautions for radioactive materials; membrane processes; enzyme linked immuno-sorbent assay (ELISA);
differential thermal analysis (DT A); ultracentrifugation.
Practical
Preparation of methyl esters of fatty acids of milk fat followed by their GLC separation and estimation;
TLC separation of amino acids; determination of Kav of a macromolecule using sephadex; preparation of a
buffer and measurement of its pH electro-metrically and using indicators; SDS gel electrophoresis and
molecular weight determination; determination of absorption spectra of BSA and demonstration of Beer's
law; determination of sodium and potassium by flame photometry; ultracentrifugal separation of milk and
preparation of micellar casein; ion-exchange chromatography separation of α-lactoglobulin; and β-
lactoglobulin;
Suggested Readings
Cooper, T.G. 1977 The Tools of Biochemistry, John Wiley & Sons, U.S.A.
Clark, J.M. and Switzer, R.L 1977 Experimental Biochemistry; W.H.Freeman & Co, U.S.A. Stock, R.
and e.B.F. Chromatographic methods; Rice, Chapman and Hall. u.K.
Thouchstone, J.e. and Dobbins, M.F. 1978 Practice of Thin Layer Chromatography, John Wiley & Sons,
USA
Weir, D.M. 1978 Handbook of Experimental Immunology, Vol. I Blackwell Scientific Publication, U.K.
2. Dairy Plant Management 2 + 1
Dairy organization and need of management; general management concept, qualities and role of dairy
managers; decision making; planning and implementation; organization design and structure; human
resource management; work authority relationship; motivation and co-ordination; production planning and
inventory control; plant operation efficiency; productivity; financial management; organization
communication (MIS, database and role of computers); social and business economics; industrial relations
9
and human values; labour laws; trade unionism; co-operatives; worker's participation in management;
Concept of Quality Circles; marketing concepts; label laws, advertising and exports, forecasting and future
planning; industrial R&D; new product development; new concepts in quality assurance (HACCP; ISO
certification); patent laws, pollution control laws in relation to dairy plants.
Practical
Case studies (HACCP and ISO certification); Quality circles: Effective communication (Text; verbal and
electronic); Project Planning and Implementation; Quantitative Decision making; Operation research
problems; Milk procurement survey; Market survey; Response of consumers on new products and
marketing survey; International export; market and problems; PERT and CPM problems; Production
control software; Financial control systems/ software; Marketing; multimedia and advertising; Marketing;
presentations and advertising; Project planning and presentations.
Suggested Readings
Singh, M.K. and Mahadevan, A. 1990. Project Evaluation and Management. (Ed.) Discovery Pub. House,
New Delhi.
Monappa, A. and Saiyadain, Mirza, S. 1995.Personnel Management. Teta McGraw-Hill Pub.Co. Ltd., New
Delhi.
Gowda, J.M. 2001.Management Accounting Himalaya Publishing House, Delhi. Pierson, M.D. and Corlett,
D.A.Jr. 1992. HACCP Principles and Application. Van Nostrand Reinbold, New York.
AB-523 �utritional Biochemistry 2 + 0
Nutritive value of foods with special reference to milk and milk products, nutritional requirements,
bioavai1ability of nutrients. Vitamins and their role in growth health and disease, clinical and biochemical
features of other disorders (protein-calorie malnutrition, diabetes and cardiovascular disease), their
prevention and correction through diet therapy. Diet and tissue enzymes. Assessment of protein quality,
food toxins, nutrition and drug metabolism, functional evaluation of nutritional status with respect to
certain nutrients:
Suggested Readings
Renner, E. Milk and Dairy Products in Human Nutrition. W, Gmbh, Velkswirthschafslicher Verlag,
Munchen, ISBN.
Goodhart, R.S. and Shils, M.E. Modem Nutrition in Health and Diseases. Verghese Bros., Bombay.
Davidson, S.S., Passmore, R., Brock, J.F.and Truswell, A.S. Human Nutrition and Dietetics. Churchill
Living Sten, Edinburgh.
Swaminathan, M. Essentials of Foods and Nutritions, Vol. 1 & 2. Ganesh & Co., Madras.
Tannenbaum, S.R. Nutritional and Safety Aspects of Food Processing. Marcel Dekker Inc., New York.
ES-529 Statistics for Industrial Applications 2 + 1
Statistical methods in industrial applications; analysis of variance; transformations; partial and multiple
correlation and regression; Ranking techniques; introduction to discriminant analysis; statistical basis for
drawing scientific inferences from experimental data; principles of experimental design-industrial
experimentation; basic designs-CRD; RED & LSD; missing plot technique; factorial experiments-main
effects and interactions; 2n series and mixed factorial experiments; experimental designs in sensory
evaluation; introduction to statistical quality control; control charts for variables; mean and range charts;
statistical basis; rational sub-group; control charts for attributes 'np'; 'p' and 'c' charts; fundamental concepts
of acceptance sampling plans; single; double and sequential sampling plans; use of sampling inspection
tables for selection of single and double sampling plans; introduction to sampling techniques and their
application to consumer preference studies.
Practical
Analysis of variance-one way and two way classification; partial and multiple correlation and regression;
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rank correlation and coefficient of concordance; analysis of industrial experiments Use of CRD; RBD and
LSD; missing plot technique; factorial experiments - 22 and 23; mixed factorial experiments; control
charts for variables; control charts for attributes; single sampling plan- OC and AOQ curves; sequential
sampling plan; use of sampling inspection tables; and different methods of selecting samples;
Suggested Readings
Snedecor; G. W. and Cochran, W.G. 1968 Statistical Methods Oxford and IBH Publishing Co., New Delhi
Cochran, W. G. 1972 Sampling Techniques. Wiley Eastern Pvt. Ltd., New York
Cochran, W. G. and G.M. Cox 1962 Experimental Designs Asia Publishing House, Bombay Grant and
Leavenworth 1972 Statistical Quality Control, McGraw Hill, New Delhi
Indian Standards Institute 1986 New Delhi Handbook on Statistical Quality Control.
CS-521 Computer Software Packages for Statistical Analysis 2 + 1
General data analysis requirements in dairy research; Introduction to statistical and other standard software
packages (SYSTAT; SPSS; MATLAB and MS-Excel; data preparation and job control commands for
statistical analysis of data pertaining to Descriptive Statistics; Tests of significance- t-test; Chi-square test;
Analysis of variance (ANOVA); Basic experimental designs – CRD; RBD & LSD; Factorial designs;
Correlation; Simple and Multiple Linear Regression, Curvilinear Regression; Stepwise Regression;
Discriminant Analysis; (Graphic features of the above listed software packages) Linear Programming
(using LINDO/LINGO) software packages; Least-squares analysis; Prediction models using neural
networks.
Practical
Statistical software package and their operations; Data preparation and data generation; Import and export
of data from spreadsheet and database packages; Application of software packages to the problems related
to Descriptive Statistics; Tests of significance - t; Chi-square and F-test Analysis of variance (ANOVA);
Correlation; Simple and Multiple linear Regression; Curvilinear Regression; Stepwise Regression;
Discriminant analysis; Graphic features of the above listed software packages; Linear programming; Least-
squares analysis; Neural network models for prediction and classification.
Suggested Readings
Snedecor, G.W. and Colcharn, W.G. 1967. Statistical Methods; Oxford and IBH Publishing Co., New
Delhi
Levin, Richard I, and Rubin David S. 1994. Statistics for Management, Prentice Hall of India, New Delhi
Amble, V.N. 1975. Statistical Method in Animal Science, Indian Society of Agricultural Statistics, ICAR,
New Delhi
Singh R. K. and Chaudhary, B.D. 1979. Biometrical methods in quantitative genetic analysis Genetic
Analysis, Kalyani Publishers, Ludhiana
Swarup, Kanti, Gupta, P.K. and Man Mohan 1997. Operations Research. Sultan Chand and Sons, New
Delhi.
RD-521 Research Documentation 1 + 1
Library as information centre; information technology; CD ROM data search; abstracts and abstracting
techniques; the concept of documentation; international network/Indian scene; information retrieval
systems; MIS; science citation; web internet search; presentation of results of research; different kinds of
scientific writings: writing of scientific reports, thesis, research papers etc; oral and poster presentation of a
paper in conferences/symposia; standard abbreviations used in proof reading; printing technology and
corrections of manuscripts.
Practical
11
Different schemes of classification & various cataloguing codes for arranging documents; use of
catalogues & classification; types of libraries & standard sources of information; familiarity in use of
standard sources of information; exercise in abstracting of research articles; demonstration of photography;
photocopying; printing; preparation and handling of audio-visual aids; preparation of multimedia; exercise
in proof corrections; research paper writing & interpretation; CD-ROM search; and Internet search.
Suggested Readings
Balaguru, T. 1996. Management Information Systems for Agricultural Research, NAARM, Hyderabad.
Chhotey Lal 1998. Agricultural Libraries & Information Systems: A Handbook for Users. R.K. Techno
Science Agency, New Delhi.
Guha, B. 1987. Documentation & Information. World Press Pvt. Ltd., Kolkatta
Kaula, P.N. etc. 1996. International & Comparative Librarianship and Information Systems. B.R.
Publishing Corpn., Delhi.
Venkatasubramanian, V. 1999. Introduction to Research Methodology in Agricultural & Biologial
Sciences. New Century Book House, Chennai.
Sharma, P.S.k. 1987 Library & Society. Ess Ess Publication, New Delhi. CBF Style Manual Published by
Council of Biology, Maryland.
12
DAIRY MICROBIOLOGY
A.I CORE COURSES
DM-511 Microbial Morphology and Taxonomy 2 + 1
Principles of classification and taxonomy of microorganisms: immunological and genetic attributes,
phylogenetic relationship, role of 16s rRNA, development of dendrograms; application of computer
software programmes in bacterial classification; microbial morphology and ultrastructure (prokaryotes and
eukaryotes): cell wall: structure, chemical composition, synthesis and inhibition, cell membrane,
cytoplasmic inclusions, cell appendages, capsule, flagella, pili, sporulation structure of endospore,
composition and function of spore constituents, induction and germination, classification and morphology
of viruses, bacteriophages, life cycle of lambda phage.
Practical
Differential staining; gram, spore, acid-fast staining, cell wall, flagella, nucleoids, capsule, and
inclusion/storage bodies, preparation of bacterial protoplasts and spheroplasts; SEM/TEM of bacterial
cells/viral particles (demonstration); application of computer software in bacterial identification
Suggested Readings
Alberts, B., Johnson, A., Lewis, I., Raff, M., Roberts, K. and Walter, P. 1998, Essentials of Cell Biology.
Garland Publishing Inc., New York, USA.
Black J.G. 1999. Microbiology: Principles and Explorations. 4th ed. John Wiley and Sons, New York,
USA.
Holt,J.G., Krieg, N.R., Sneath, P.H.A., Staley, J.T. and Williams, S.T. 1997. Bergey's Manual of
Determinative Bacteriology (9th edition). Williams and Wilkins, Baltimore, Maryland, USA.
Madigan, M. T., Martinko, J.M. and Parker, J. 1999. Brock Biology of Microorganisms. Prentice Hall,
London
Salyers, A.A. and Whitt, D,D. 2001. Microbiology - Diversity, Disease and the Environment. Fitzgerald
Science Press. Inc, USA.
DM-521 Starter Cultures and Genetic Improvement 2 + 1
History and taxonomy of starter cultures; biochemical and genetic characterization of lactic acid bacteria;
carbohydrate, citrate and protein metabolism of starter cultures; starter types: single, mixed and multiple
strain starter cultures; propagation and preservation of starter cultures; commercial starter preparations:
concentrated and superconcentrated starters; genetics of starter cultures: plasmids and plasmid instability;
industrially significant genes and systems; genetic modification of lactic acid bacteria through
transduction; conjugation; protoplast transformation; electroporation and chromosomal integration,
transposons and insertion sequences; growth inhibition of lactic acid bacteria by antibiotics, bacteriocins;
immunoglobulins and bacteriophage: sources, types and characteristics of phages associated with starters,
phage control during starter handling and growth, mechanisms of phage resistance in LAB; probiotic cul-
tures, health and nutritional benefits, requirements for ability to survive and grow in the intestine, control
of intestinal infections, role of starter cultures in cheese making and ripening of different cheese varieties;
associated starters; microbiology of Cheddar cheese, Cottage cheese, Blue cheese, Swiss cheese and
Mozzarella; microbiological defects in cheese; role of starter cultures in preparation of fermented milks
like yoghurt, dahi, kefir and kumiss.
Practical
Isolation of lactococcal cultures from fermented milks; examination of purity and activity of starter
cultures; preservation of starter cultures by freeze drying, microdrying and other methods; preparation of
concentrated starters and quality evaluation; Inhibition of starters by antibiotic residues and other
inhibitors; plasmid profiles of some lactococcal cultures; identification of lactic starters by molecular
biology techniques (demonstration); conjugal transfer of plasmids in lactococci; production of bacteriocins
13
by LAB.
Suggested Readings
Cogan, T.M. and Accolas, J.P. 1995 Dairy Starter Cultures, VCH Publishing Inc., New York, USA.
Law, B.A. 1997. Microbiology and Biochemistry of Cheese and Fermented Milks, 2nd Ed., Blackie
Academic and Professional, London, UK.
Marth, E.M. and Steele, J.L.1998. Applied Dairy Microbiology, Marcel Dekker Inc., New York, USA.
Robinson, R.K. 1998. Developments in Food Microbiology Vol 4, Elsevier Applied Science, New York,
USA.
Salminen. S. and Wright, A.V., 1998. Lactic acid Bacteria, Marcel Dekker Inc., New York, USA.
DM-522 Microbial Genetics 2 + 1
Macromolecules and maintenance of genetic information; genetic code, DNA replication, mutagenesis,
mutations and mutants; gene expression and its regulation, 'lac' and 'trp' operon models; plasm ids in
bacteria, transfer of plasmid DNA, F, Rand Col plasm ids; transposable elements; bacterial recombination -
transformation, conjugation and transduction; fundamental aspects of genetic engineering, vectors,
restriction enzymes, gene cloning and application of genetic engineering.
Practical
Isolation and molecular sizing of plasmid DNA from E. coli by miniprep; calcium chloride induced
transformation of E. coli hosts with plasm ids; induction of random mutation in E. coli by UV irradiation
and NTG; conjugation in bacteria and genetic mapping by interrupted mating, induction of b-galactosidase
in E. coli; generalized transduction in bacteria.
Suggested Readings
Bery and Singer, 1993. Dealing with Genes: The Language of Heredity. University Science Books, Milk
Valley, CA, USA.
Brock, T.D. 1990. The Emergence of Bacterial Genetics. Cold Spring Harbor Laboratory Press, New York,
USA.
Gardner, E.J., Simmons, M.J. and Snustad, D.P. 1991. Principles of Genetics. 8th Ed. John Wiley and Sons,
Inc., New York, USA.
Lewin, B. 2000. Gene VII. Oxford University Press. USA.
Maloy, S.R., J.E. Cronan Jr.J.E. and Jones, D. 1994. Freifelder Microbial Genetics, 2nd Ed. Jones and
Bartlett Pub., USA
DM-512 Microbial Physiology 2 + 1
Bacterial growth: growth phases and kinetics, synchronous, continuous, and associative growth; factors
affecting bacterial growth; growth measurement, growth inhibition; mechanism of action of antimicrobials;
grouping of bacteria on the basis of temperature requirements, osmotic pressure (salt, sugar concentration)
active and passive transport; bacterial nutrition; nutritional groups of bacteria; role of growth factors;
electron transport chain: components of respiratory chain; mechanism of electron transport; bacterial
photosynthesis; cyclic and non-cyclic photophosphorylation; inhibition of electron transport chain.
Practical
Measurement of bacterial growth by direct methods (cell number, SPC, DMC) and indirect methods
(turbidometric methods, MPN, cell mass; total nitrogen, etc.); preparation of growth curve; determination
of generation time; determination of cell activity at different growth phases; carbohydrate fermentation;
acid production/pH alteration; starch, lipid, casein and gelatin hydrolysis; effect of different factors viz.,
physical (temperature, pH, osmotic pressure, surface tension), chemical (dyes, antibiotics, phenol) and
nutritional (amino acid supplements, vitamin supplements, protein hydrolysates, cas-amino acids) on
14
bacterial growth.
Suggested Readings
Bruijn Frans J. de 1998. Bacterial Genomes; Physical Structure and Analysis. Chapman and Hall, London,
UK.
Caldwell, D.R. 1995. Microbial Physiology and Metabolism. WMC Brown Publishers, USA.
Koch, L. 1995. Bacterial Growth and Form. Chapman and Hall, New York, USA.
Moat A.G. and Foster, J.W. 1995. Microbial Physiology, 3rd Ed. John Wiley and Sons. New York, USA.
Nicoloi S. Panikov. 1995. Microbial Growth Kinetics. Chapman and Hall, London, UK.
A.2 OPTIO�AL COURSES
DM-513 Analytical Techniques in Microbiology 2 + 1
Microscopy: principles, design and functions of bright field, dark field, phase contrast and fluorescence
microscope; study of various accessories for instructional purposes; care and maintenance of microscopes;
measurement of microscopic objects: principle, design and application of transmission and scanning
electron microscopes for the study of sub-cellular organisation and microstructure of dairy foods;
principles and use of ultrasonic disintegrater and other cell disruption techniques; anaerobic culturing
techniques for isolation of obligate/facultative anaerobic organisms; use of animal models in toxicity
studies; design and application of biofermenters; polymerase chain reaction (PCR) technique for
identification of microorganism.
Practical
Familiarization with the construction and design of a compound microscope; use of light microscope
accessories; microscopic analysis of different types of bacteria by bright field and dark field; phase contrast
and fluorescence microscopes; working demonstration of SEM:TEM and critical point dryer, sputter coater
and ultra microtome sample preparation for the study of microstructure of dairy products; disruption of
bacterial cells by ultrasonification; demonstration of anaerobic culturing techniques, demonstration of use
of animal models in toxicity studies, working demonstration of biofermenters; demonstration of PCR
technique as a tool for identification and characterization of microorganism.
Suggested Readings
Bozzola, U. and Russell, L.D. 1992. Electron Microscopy: Principles and Techniques for Biology.
Jones and Bartlett Publishers, Boston, USA.
Gerhardt, P., Murray, R.G.E., Wood, W.A. and Kreig, N.R. 1994. Methods for General and Molecular
Biology. ASM Press, Washington DC, USA.
Hartley, W.G. 1993. The Light Microscope; It's Use and Development Science PublishingCo., Oxford,
U.K.
Herman, B. 1998. Fluorescence Microscopy, Bios Scientific Publishers Oxford, U.K. Singer, S. 2001.
Experiments in Applied Microbiology, Academic Press, New York, USA.
DM-523 Microbiological Quality Assurance for Dairy Industry 2 + 1
Microbiological hazards and their control in dairy industry; relative importance of traditional inspection,
HACCP, SAFE and SCAP in quality control; specific HACCP application for pasteurized milk;
establishment of microbiological specifications; two and three ciass attribute plans for sampling; rapid
detection methods including commercial detection kits; automated detection techniques for dairy industry;
principles of personnel hygiene and food handling; method of disease transmission; sanitary handling of
dairy foods; trends in food borne diseases and implications; principles of safety in a food microbiology lab.
Practical
15
Detection of microbial contamination in milk and milk products through on line testing; Identification of
CCPs for hazard analysis; identification of prominent genera for personnel hygiene; fluorescence
microscopy for rapid detection of microflora; detection of recently emerged dairy pathogens like, Yersinia
and Listeria.
Suggested Readings
Marshall, R.T. 1992. Standard Methods for the examination of Dairy Products. APHA, Washington 0 C,
USA.
Morgan, MRA, Smith, C.J. and William, P.A. 1992. Food Safety and Quality Assurance. Elsevier Applied
Science, London, UK.
Mortimore S. and Wallace. C. 1994. HACCP: A Practical Approach. Chapman and Hall, London, UK.
Patel, P. 1994. Rapid Analysis Techniques in Food Microbiology. Blackie Academic and Professionals,
London, UK.
Vanderzant, C. and Spiltstoesser. D.F. 1992. Compendium of Methods for the Microbiological
Examination of Foods. APHA, Washington DC, USA.
DM-514 Environmental Microbiology and Pollution Control 2 + 1
Microorganisms as components of the environment and their role in nutrient cycling; extreme
environments and microbial ecology; microbes in aquatic and terrestrial environment; microorganisms as
indicators of environmental pollution; bioorganic pollution; greenhouse effect and global warming;
microbial toxicants and pollutants, and their bio-degradation; biodegradation of plastics and oil pollution
and role of microorganisms in oil spill degradation; biofouling and biofilms; bioremediation and metabolic
engineering; water pollution and control with the help of microbes; biological treatment of food industry
wastes; issues concerning release of genetically engineered microorganisms in the environment;
environmental laws.
Practical
Determination of BOD in industrial wastes; determination of composite microflora of selected
environmental samples; detection of low levels of xenobiotics, microbial toxins and residual antibiotics in
environmental samples; isolation of bacteria capable of degrading organic and microbial pollutants from
environmental samples, isolation of bio-indicators by membrane filtration; a visit to sewage and sludge
treatment plant.
Suggested Readings
Akkeraus, Antoon, D.L., Elsas, J.D.V., Bruijn, F.J. 1998. Molecular Microbial Ecology Manual; Kluywer
Academic Publishers, The Netherlands.
Alexander, M. 1992. Microbial Ecology; John Wiley and Sons, New York, USA.
Atlas, R.M. 1993. Microbiology - Fundamentals and Applications. (3rd Ed), Macmillan Publishing Co.,
New York, USA.
Bitton, G. 1994. Waste Water Microbiology; John Wiley and Sons, New York, USA.
Mitchell, R. 1995. Introduction to Environmental Microbiology. (8th Ed.); Prentice-Hall of India (P) Ltd.
New Delhi, India.
DM-524 Microbiology of Fodder, Feed and Dairy Waste Utilization 2 + 1
Role of microorganisms in soil fertility improvement and organic matter decomposition; carbon and
nitrogen cycles; biological nitrogen fixation; microbial inoculants for fodder crops; silage fermentation:
microbial chemical and biochemical changes, use of additives and inoculants, losses during ensiling; rumen
microorganisms: types and characteristics; role in rumen metabolism; bioconversion of crop residues by
solid state fermentation. single cell protein production; mycotoxins in feed and their control; anaerobic
digestion of animal excreta and plant wastes for biogas production; solid and liquid waste management for
resource recovery and pollution control.
16
Practical
Quantitative estimation of soil and rhizosphere microorganisms; measurement of soil microbial activity
with organic matter supplement through C02 evolution, ammonification and nitrification in soil; isolation
of Rhizobium inoculant from fodder legume nodules; preparation of Rhizobium inoculant for fodder
legume; silage sampling technique and estimation of moisture and pH in silage; enumeration of silage
microorganisms; estimation of lactic, acetic and butyric acids in silage; sampling technique of rumen liquor
and direct microscopic count of protozoa; anaerobic cultivation techniques for bacterial and fungal counts
in rumen liquor; SCP production from cellulose in submerged fermentation; bioconversion of straw by
solid state fermentation; study of anaerobic digestor and biogas production; estimation of BOD and COD in
dairy farm effluent.
Suggested Readings
Hobson P.N. and Stewart C.S. 1997. The Rumen Microbial Ecosystem, 2nd Ed. Chapman and Hall,
London, UK.
Pandey, A. 1994. Solid State Fermentation. Wiley Eastern Limited, New Delhi, India. Tate, R.L.
2000 Soil Microbiology. 2nd Ed. John Wiley and Sons, New York, USA.
Wallace, R.J. and A. Chesson, 1995. Biotechnology in Animal Feeds and Animal Feeding, VCH Pub.,
Weinheim, Germany.
Woolford, M.K. 1984. The Silage Fermentation. Marcel Dekker Inc., New York, USA.
DM-525 Microbial Spoilage and Safety of Dairy Products 2 + 1
Influence of processing on microbial flora of milk: bactofugation, homogenization and thermal treatments;
role of thermophilic and thermoduric microorganisms in milk spoilage, significance of spore formers;
effect of low temperature storage: role of psychrotrophs and their enzymes (lipases and proteases); other
types of microbiological spoilage and defects, and control measures; public health concerns associated with
residual antibiotics, sanitizers and pesticides, and their biodegradation, health concerns regarding
development of antibiotic resistance in microorganisms as associated with milk, effect of milk processing,
and their rapid detection, prevention and control; safety aspects of high risk dried milk formulations
including probiotics for infants and immuno-compromised individuals; effect of reconstitution on
microbiological quality of infant foods: role of water, feeding bottles and nipples in their safety;
development of flavour in cream and butter, undesirable microbial changes in these products during storage
and their control; diseases transmitted through milk and milk products; current concerns including
aflatoxins; emerging pathogens; dairy product safety systems including HACCP concept and GMP;
longitudinally integrated processing for safety and quality; microbiological analytical techniques for quality
monitoring, guidelines, specifications and standards for foods; microbiological criteria; sampling
programmes, accreditation bodies, regulatory bodies (national and international); I SO-9000 as quality
specification; trends in food consumption.
Practical
Microbiological quality evaluation of raw and pasteurized milk: SPC, DMC, dye reduction (MBRT, RRT),
MPN and their correlation; tests for abnormal milks - mastitis, brucellosis, antibiotic and pesticide residues
in milk and milk products; evaluation of dairy detergents and sanitizers; sample preparation and quality
evaluation of dairy products: SPC, coliforms, aerobic spore counts, yeasts and moulds etc.; detection of
common dairy pathogens from milk and milk products: E. coli, S. aureus, B. cereus, Enterococcus
faecalis, Salmonella sp. Clostridium perfringens; shelf life studies of dairy products; effect of storage
conditions and packaging material; role of preservatives.
Suggested Readings
Robinson R.K. 1990. Dairy Microbiology Vol.1 & 2, Applied Sciences Pub., London, UK.
Yadav, J.S., Batish, V .K. and Grover, S. 1993. Comprehensive Dairy Microbiology Metropolitan Pub.,
Delhi, India.
Marth, E.H. and Steele. J.L. 1998. Applied Dairy Microbiology, Marcel and Dekker Inc., New York, USA.
17
Marshall, RT 1992. Standard Methods for the Examination of Dairy Products. 16th Ed., APHA,
Washington DC, USA.
Frazier, W.C. and Wessthoff, D.C., 1988. Food Microbiology, Tata McGraw Pub. N. Delhi.
DM-515 Microbiology of Processed Foods 2 + 1
Microbial ecology of foods: main concerns, ecology of prepared foods; mechanisms of stress induced
injury to microorganisms in processed milk; repair of injured organisms, enumeration of stressed cells;
predictive modeling for food spoilage and safety; low temperature food preservation; thermal processes for
shelf stable products; recent concepts in irradiation technology; safety aspects and social acceptance; new
methods for controlling spoilage of foods; modified atmosphere packaging and shelf life of processed
foods; microbiological safety considerations; intermediate moisture foods and hurdle concept; new
prospects and problems in fermented foods; potential applications of fermentation processes; genetically
engineered products and ecosystem; effects of technological changes.
Practical
Production of antimicrobial substances; application of bacterial consortia in fermented foods; application of
hurdle concepts for enhanced shelf stability of processed foods; induction of bacterial cell injury and
recovery of injured cells; effect of modified atmosphere packaging on quality and shelf life of processed
foods; production of beverage by whey fermentation; extending shelf life of foods by food grade bio-
preservatives; development of predictive equation and its validation for food safety; assessment of
pollution in food processing environment and biodegradation of xenobiotics in foods.
Suggested Readings
Doyle, M.P., Benchat, L.R., Montville, T.J. 1997. Food Microbiology: Fundamentals and Frontiers. ASM
Press, Washington, DC, USA.
Hui, Y.H. and George G. Khachatourians, 1995. Food Biotechnology: Microorganisms, VCH Publishers
Inc., New York, USA.
Marwaha, S.S. and Arora, J.K. 2000. Food Processing Biotechnological Application, Asia-Tech. Pub. New
Delhi, India.
Robinson, R.K. (1998). Developments in Food Microbiology Vol. 1, 2 and 3, Elsevier Applied Sciences,
New York, USA.
Susan, K.H. and Theodore P.L., 1986. Biotechnology in Food Processing. Noyes Publications, New Jersey,
USA.
DM-526 Microbial Pathogens and Toxins 2 + 1
Host defense mechanism against pathogens: paradigms, immunological response, and phagocytosis;
precautions for working with pathogenic microorganisms; serogroups and serotype classification; rapid
detection techniques for food borne pathogens and their toxins; cell cultures and cytopathogenesis; host
receptors, invasion and intracellular survival; median lethal and infectious doses in animal model system;
development of vaccines; chemical structure and mode of action of bacterial toxins, Vibrio cholerae,
Corynebacterium diphtheriae, Listeria monocytogenes, Yersinia enterocolitica; chemical structure and
biological activity of fungal toxins; aflatoxins, citrinin, ochratoxins, trichothecenes,viral haemolysins;
mechanisms of cell damage, hepatitis, poliomyelitis, other enteroviruses.
Practical
In-vitro evaluation of bacterial toxins by immunological techniques like slide agglutination, tube
agglutination, gel diffusion assay; In-vivo evaluation of bacterial toxins using animal models like rabbit
ideal loop technique, dermal necrosis, infant mice, purification of staphylococcal toxins by
chromatographic techniques.
Suggested Readings
Aktories Klaus 1997. Bacterial Toxins: Laboratory Manual (Tools in Cell Biology and Pharmacology)
18
Chapman and Hall, London, UK.
Clark, V.L. and Baroil, P.M. 1997. Bacterial Pathogens. Academic press, New York, USA. Morgan, MRA,
Smith C.J. and William, P.A. 1992. Food Safety and Quality Assurance Elsevier Applied Science, London,
UK.
Salyers, A.A. and Whitt, D.D. 1994. Bacterial Pathogens is; A Molecular Approach. ASM Press,
Washington DC, USA.
Steinhart, C.E., Doryle, M.E., Cochgranes, B.A. 1995. Food Safety. Marcel Dekker, New York, USA.
DMRT-527 Research Techniques 3+1
Electrophoresis: principle and types, isoelectricfocussing; chromatography: column, TLC, GLC, HPLC,
gel-permeation, ion-exchange, affinity, chromatofocussing; spectrophotometry:UV, visible, IR and flame
photometry; potentiometry: principle, various electrodes; electrometric measurements of pH, buffers;
radiotracer technique: nuclear transformation, nuclear decay, measurement of radioactivity and safety
precautions for radioactive materials; membrane processes; enzyme iinked immuno-sorbent assay (ELISA);
differential thermal analysis (DTA); ultracentrifugation.
Practical
Preparation of methyl esters of fatty acids of milk fat followed by their GLC separation and estimation;
TLC separation of amino acids; determination of Kav of a macromolecule using sephadex; preparation of a
buffer and measurement of its pH electro-metrically and using indicators; SDS gel electrophoresis and
molecular weight determination; determination of absorption spectra of BSA and demonstration of Beer's
law; determination of sodium and potassium by flame photometry; ultracentrifugal separation of milk and
preparation" of micellar casein; ion-exchange chromatography separation of α-lactoglobulin; and β-
lactoglobulin.
Suggested Readings
Cooper, T.G. 1977 The Tools of Biochemistry, John Wiley & Sons, U.S.A.
Clark, J.M. and Switzer, R.L 1977 Experimental Biochemistry; W.H.Freeman & Co, U.S.A.
Stock, R. and C.B.F. Chromatographic methods; Rice, Chapman and Hall. U.K.
Thouchstone, J.C. and Dobbins, M.F. 1978 Practice of Thin Layer Chromatography, John Wiley & Sons,
USA
Weir, D.M. 1978 Handbook of Experimental Immunology, Vol. I Blackwell Scientific Publication, U.K.
B. SUPPORTI�G COURSES
ES-529 Statistics for Industrial Applications 2 + 1
Statistical methods in industrial applications; analysis of variance; transformations; partial and multiple
correlation and regression; Ranking techniques; introduction to discriminant analysis; statistical basis for
drawing scientific inferences from experimental data; principles of ex perimental design-industrial
experimentation; basic designs-CRD; RBD & LSD; missing plot technique; factorial experiments-main
effects and interactions; 2n series and mixed factorial experiments; experimental designs in sensory
evaluation; introduction to statistical quality control; control charts for variables; mean and range charts;
statistical basis; rational sub-group; control charts for attributes 'np'; 'p' and 'c' charts; fundamental concepts
of acceptance sampling plans; single; double and sequential sampling plans; use of sampling inspection
tables for selection of single and double sampling plans; introduction to sampling techniques and their
application to consumer preference studies.
Practical
19
Analysis of variance-one way and two way classification; partial and multiple correlation and regression;
rank correlation and coefficient of concordance; analysis of industrial experiments Use of CRD; RBD and
LSD; missing plot technique; factorial experiments - 22 and 23; mixed factorial experiments; control
charts for variables; control charts for attributes; single sampling plan- OC and AOQ curves; sequential
sampling plan; use of sampling inspection tables; and different methods of selecting samples;
Suggested Readings
Snedecor; G. W. and Cochran, W.G. 1968 Statistical Methods Oxford and IBH Publishing Co., New Delhi
Cochran, W. G. 1972 Sampling Techniques. Wiley Eastern Pvt. Ltd., New York
Cochran, W. G. and G.M. Cox 1962 Experimental Designs Asia Publishing House, Bombay Grant and
Leavenworth 1972 Statistical Quality Control, McGraw Hill, New Delhi
Indian Standards Institute 1986 New Delhi Handbook on Statistical Quality Control.
CS-521 Computer Software Packages for Statistical Analysis 2 + 1
General data analysis requirements in dairy research; introduction to statistical and other standard software
packages (SYST A T; SPSS; MS-Excel); data preparation and job control commands for statistical analysis
of data pertaining to t-test; Chi-square test; analysis of variance (ANOY A); basic experimental designs -
CRD; RBD & LSD; factorial designs; partial and multiple correlation and regression; discriminant
analysis; linear programming; (using LINDO/ LINGO) software packages; least-squares analysis.
Practical
Statistical software packages and their operations; data preparation and data generation; import and export
of data from spreadsheet and database packages; application of software packages to the problems related
to: tests of significance (t; Chi-square and 'F -test); analysis of variance (ANOVA); partial and multiple
correlation and regression; discriminant analysis; linear programming problem; and least-squares analysis.
Suggested Readings
Snedecor, G. W. and Cochran, W.G. 1967. Statistical Methods; Oxford and IBH Publishing CO., New
Delhi.
Levin, Richard I. and Rubin David, S. 1994. Statistics for Management, Prentice Hall of India, New Delhi.
Amble, V.N. 1975 Statistical Methods in Animal Science, Indian Society of Agricultural Statistics, ICAR,
New Delhi.
Singh, R.K. and Chaudhary, B.D. 1979. Biometrical methods in quantitative genetic analysis. Genetic
Analysis, Kalyani Publishers, Ludhiana.
Swarup, Kanti, Gupta., P.K. and Man Mohan 1997. Operations Research. Sultan Chand and Sons, New
Delhi.
RD-511 Research Documentation 2 + 1
Library as information centre; information technology; CD ROM data search; abstracts and abstracting
techniques; the concept of documentation; international networkllndian scene; information retrieval
systems; MIS; science citation; web internet search; presentation of results of research; different kinds of
scientific writings: writing of scientific reports, thesis, research papers etc; oral and poster presentation of a
paper in conferences/symposia; standard abbreviations used in proof reading; printing technology and
corrections of manuscripts.
Practical
Different schemes of classification & various cataloguing codes for arranging documents; use of catalogues
& classification; types of libraries & standard sources of information; familiarity in use of standard
sources of information; exercise in abstracting of research articles; demonstration of photography;
photocopying; printing; preparation and handling of audio-visual aids; preparation of multimedia; exercise
in proof corrections; research paper writing & interpretation; CD-ROM search; and Internet search.
20
Suggested Readings
Balaguru, T. 1996. Management Information Systems for Agricultural Research, NAARM, Hyderabad.
Chhotey Lal 1998. Agricultural Libraries & Information Systems: A Handbook for Users. R.K. Techno
Science Agency, New Delhi.
Guha, B. 1987. Documentation & Information. World Press Pvt. Ltd., Kolkatta
Kaula, P.N. etc. 1996. International & Comparative Librarianship and Information Systems. B.R.
Publishing Corpn., Delhi.
Venkatasubramanian, V. 1999. Introduction to Research Methodology in Agricultural & Biologial
Sciences. New Century Book House, Chennai.
Sharma, P.S.k. 1987 Library & Society. Ess Ess Publication, New Delhi. CBF Style Manual Published by
Council of biology, Maryland.
Ph.D. Core Courses
DM-611 : Microbial Diversity and Physiology
Bacterial Growth, Growth phases, Factors affecting growth, Growth Kinetics, Continuous
Culture, Growth yields, Influence of natural environment on microbial growth.
Prokaryotic cellular structures, biosynthesis, Sporulation and regulatory mechanisms.
Autotrophy, Nutritional classification, Economic importance of autotrophs.
Environmental selection and microbial succession of different species, Interactions of
microbes with the environment
Population interactions and modifications via microbial metabolisms, Regulation of
metabolic and catabolic pathways
Membrane transport systems, Active and Passive transport, Group translocation,
Models
Suggested readings :
1. Microbial Physiology, Foster JW & Spector MP Moat, Albert, G. (2002)
Wiley-Liss, New York.
2. Microbial Physiology & Metabolism, Caldwell Daniel R. (1995), Dubuque:
Wm G Brown Publications.
3. Principles of Biochemistry, Lehninger A.L., Nelson DL, Cox MM, CBS
Publishers (2003).
4. Microbiology, Prescott DM, Hardy JP, Klien DA, McGraw Hill Publications
(2005).
21
5. Brocks’s Biology of Microorganisms, Madigan MT, Martink J.M, and Parkar
S (2003), Prentice Hall, New Jersey, USA.
22
DM-612 Advances in Microbial Genetics
1. Essentials of Genetics
2. Nucleic Acis : Structure, Function and Genetic Code; DNA Repliction
3. Mutations, DNA Damage and Repair; Site Directed Mutagenesis
4. Gene Expression, Trnscription, Translation and Regulation of Gene Expresion
(Lac, Gal and Trp operons)
5. Plasmids, Trnsposable elements
6. Genetic Recombination: Transformation, Transduction and Conjugation
7. Genetic Engineering, Restriction Enzymes, Vectors, PCR cloning, Microarray
technology, Gene Silencing and Gene knock out.
Suggested Readings :
1. Dale, J and Schantz, M.V. 2002. From gene to genome: concepts and
application of DNA technology. New John Wiley and Sons, USA
2. Synder, Lassy and Champness, W. 2003. Molecular Genetics of Bacteria (2nd
ed) ASM Pub., Washington, DC
3. Friedberg, E. C and Walker, G.C. 1995. DNA Rpair and Mutagenesis, ASM
Press, Washington
4. Malacinski, G.M. 2003. Essentials of Molecular Biology (4th ed.) Jones and
Bartlette Publishers, Massachusettes, USA
5. Lewin, Benzamin, 2004. Gene VIII. Pearson Eduction International London.
ISBN-O-13-12-123826.
6. Winnacker, E.L. 2003. From Gene to Clones. Panima Publishing house, New
Delhi. ISBN 81-86535-40-9.
7. Hartl, Daniel, L., Jones and Elizabeth, W. 2000. Genetic Analysis of Genes
and Genomes. Jones bartkett, Publishers, London.
8. Watson, J.D. 2003. Molecular Biology of Gene (5th Ed.). The
Benjamin/Cummings Publishing. ISBN 0-805309614-4.
9. Green, J.J. and Rao, B.V., 1998. Recombinant DNA and Methodologies.
(Illustrated edition) Marcel Dekker Publisher, ISBN : 0824799895.
10. Keuzer, H. and Massey, A. 2001. Recombinant DNA and Biotechnology. (2nd
ed.) ASM press, Washington, D.C. ISBN 1-555-81-175-2.
11. Streips, U .N. and Yasbin, R.E. 2002. Modern Microbial Genetics (2nd Ed.)
John Wiey and sons, Inc. Publisher, New York, ISBN – 0-47138665-0.
23
DM-621 : Advances in Dairy and Food Microbiology
Principles of food spoilage and preservation, Influence of microbial metabolism on
properties of foods produced by fermentations, Microenvironment of foods and its effect
on important metabolic pathways of microorganisms.
Lactic acid bacteria and food fermentations, Current status of metabolism of starter
cultures, Recent concepts in starter preservation techniques, processing of fermented
foods, Lactic acid metabolisms, Modern concepts in cheese ripening, Bacteriophages and
starter rotation, Improving cultures for food fermentations by genetic manipulations/
recombinant technology.
Bacteriocins : structure, function, transport and mode of action
Current trends in food safety, Emerging pathogens, impact of new generation foods on
ecology and physiology of pathogens, Ecology and survival strategies of pathogens in
foods, New technologies in control of pathogens, Concepts in food toxicology, naturally
occurring food-borne toxicants, Current concepts in Food Quality and Safety
Management.
Suggested Reading :
1. Modern Food Microbiology
Jay, James M, Loessner, Martin J, Golden, David A., 2005, Published by
Springer.
2. Food Microbiology, 2nd Edition
M R Adams, M O Moss, 2003, Published by The Royal Society of Chemistry
3. Fundamental Food Microbiology, 3rd Edition
Bibek Ray, 2003, Published by CRC press.
4. Food Microbiology Protocols
John F T Spencer, 2001, Published by Humana Press Inc.
5. Encyclopedia of Food Microbiology (Set 1-3)
CA Batt, P Patel, R K Robinson, 1999, Published Academic Press
6. Practical Food Microbiology
D Roberts, M Greenwood, 2002, Blackwell Publishing
7. Dairy Microbiology Handbook : The Microbiology for Milk and Milk
Products, 3rd Edition.
R K Robinson, 2005, Published by Wiley.
8. Encyclopedia of Dairying (Set 1-4)
H. Roginski, F. Fuquay, P Fox 2003, Elsevier
9. Lactic Acid Bacteria
S Salminen, Atte Von Wright, Arthur Ouwehand, 2004, Marcel Dekker Inc.
10. Genetics of Lactic Acid Bacteria
Brian Wood, Philip J Warner, 2003, Springer
24
11. Applied Dairy Microbiology
E H Marth, J L Steele, 2001, Published by Marcel Dekker Inc
12. Dairy Microbiology Handbook : The Microbiology of Milk and Milk
Products, 3rd Edition, R K Robinson, 2002, Published by Wiley
DM-622 : Mechanism of Microbial Pathogenesis
Host defenses against microbial pathogens, Defenses of body surfaces, Defenses of tissue
and blood.;
Virulence factors. Colonization, Host damages, Regulation of virulence genes,
Measurement of virulence, Modulating the immune response.
Mechanisms of microbial resistance to biocides.
Microbial-host interactions; Role of colonization, Virulence factors and regulation of
virulence genes in Diphtheria, Food-borne toxicosis, Scarlet fever, Cholera, Shigellosis,
Listeriosis, Yersiniosis, Salmonella infections, gastrointestinal infections of E. coli.
Bacterial toxins, Mycotoxins, Chemical structures and biological activities, Toxic and
infective effects of food-borne viruses.
Phylogenetic diversity of pathogenic microflora, Rapid detection systems including
application of bioreporters in virulence determination, Real time evaluations.
Suggested Readings :
1. Understanding Pathogen Behaviour Viruelce, Stress Response and
Resistance, Edited by Mansel Griffiths, 2005, Published by CHIPS.
2. Bacterial Pathogenesis
Virginia Clark and Patrik Bavoil, 2003, Published by Academic Press
3. Bacterial Pathogenesis: a Molecular approach, 2nd Edition.
A A Saylers and D D Whitt, 2001, Published by ASM.
4. Microbial Foodborne Diseases : Mechanisms of Pathogenesis and Toxin
Synthesis, E A Groisman, 2000, Published by Academic Press.
5. Principles of Bacterial Pathogenesis
E A Groisman, 2000, Published by Academic Press.
6. Microbial Pathogenesis
Bruce A McClane, Bruce A Phillips, John Dowling, Timothy A Mietzner, 1999,
Published by Fence Creek Publishing.
25
DAIRY CHEMISTRY
A.I CORE COURSES
1. Physico-chemical Aspects of Milk Constituents and Milk Products 2 + 1
Kinetics: Order and molecularity of a reaction; reactions obeying the kinetics of zero, 1st, 2nd and 3rd
order; kinetics of denaturation of whey proteins; the role of enzymes as a biological catalyst; factors
affecting the rate of enzyme action- (i) concentration of substrate, (ii) concentration of enzyme, (iii)
concentration of reaction products, (iv) pH, (v) temperature, (vi) time, (vii) activators, and (viii) inhibitors;
concept of activation energy; electrochemistry: electrolytic dissociation; activity; ionic strength and salt
equilibrium in milk; dissociation constant of acids and bases; effect of ionic strength on dissociation
constant; buffers; buffer capacity and buffer index of milk; limitation of buffers; good's buffer; electrode
potential; concentration cells; electrodes reversible with respect to various cations and anions; surface and
colloid chemistry: adsorption at solid-vapour (gas) inter-phase; monolayer and multilayer adsorption;
capillary condensation; adsorption isotherms; hysteresis; water activity: sorption of water on milk con-
stitllents and milk products; its relation to stability of dairy products; adsorption at solid-liquid and liquid-
liquid interphase; Gibb's equations; Interfacial tension; micelles: definition, critical micelle concentration
formation and stability; emulsions; foams and gels-their formation, structure and stability; importance of
these phenomena in milk and milk products; properties of colloidal systems with special reference to
electrical; optical; hydrodynamics (shape and hydration) and rheological aspects; colloidal stability of
casein micelles; membrane pl1enomenon and Donnan membrane effect.
Practical
Determination of the order of hydrolysis of an ester/carbohydrate and measurement of activation energy;
determination of the progress curve obtained during the hydrolysis of P-nitrophenyl phosphate by milk
alkaline phosphatase; determination of the Michaelis constant for the digestion of casein by trypsin;
preparation of a Tris/phosphate/citrate buffer of a given molarity/ionic strength and pH; determination of
pH of the buffer; estimation of calcium ions of milk using an electrode reversible with respect to calcium
ions; determination of viscosity of condensed milk using falling ball viscometer; measuring the stability of
an oil-in-water emulsion stabilised by milk proteins; foam capacity and stability of caseins/whey proteins;
drawing of an adsorption isotherm of water on casein.
Suggested Readings
Moore, W. J. 1983 Basic Physical Chemistry Prentice Hall, New Delhi.
Ronald Jowitt 1987 Physical Properties of Foods Elsevier Appl. Sci., London
Mitchell, J. R. and. Ledward, D. A. 1986 Functional Properties of Food Macromolecules Elsevier Applied
Sciences, London.
Damodran, S. & Paraf, A. 1997 Food Proteins & their Applications Marcel Dekkar Inc., N.Y. Wong, N.P.;
Jenness, R.; Keeney, M. and Elmer, H.M. 1988 Fundamentals of Dairy Chemistry, 3rd Edn. Van Nostrand
Reinhold Co. N. Y.
Walstra, P. and Jenness, R. 1984 Dairy Chemistry and Physics John Wiley & Sons, New York
Fox, P. F. & McSweeney, PLH 1998 Dairy Chemistry and Biochemistry Blackie A&P., London.
2. Milk Carbohydrates, Minerals and Water Soluble Vitamins 2 + 1
Lactose: occurrence, isomers; molecular structure; physical properties:- crystalline habits; hydrate; lactose
glass; equilibrium of different isomers in solution; solubility; density sweetness; chemical properties:
hydrolysis; pyrolysis; oxidation; reduction; degradation with strong bases; derivatives; dehydration and
fragmentation browning reaction; oligosaccharides in milk; minerals: major and minor minerals; factors
26
associated with variation in salt composition; physical equilibrium amongst milk salts; partitioning of salt
constituents and factors affecting it; effect of various treatments on salt equilibrium; salt balance and its
importance in the processing of milk; protein mineral interactions; distribution and importance of trace
elements in milk; water soluble vitamins: thiamin; riboflavin; niacin; pantothenic acid; pyridoxine; biotin;
folacin and cynocobalamin; molecular structure; levels in milk and milk products; biological significance;
factors affecting their levels; ascorbic acid structure; relation to Eh.
Practical
Estimation of lactose in milk by volumetric; gravimetric; polarimetric and colorimetric methods;
estimation of sodium and potassium by (flame photometry); calcium and magnesium by (EDT A method);
phosphorus by colorimetric (Fiske and Subba Rao) method; citric acid and iron by colorimetric methods;
vitamin C in milk (volumetric method) and brown colouring matter;
Suggested Readings
Jenness, R. and Patton, S. 1969 Principles of Dairy Chemistry, John Wiley & Sons, New York
Wong, N.P.; Jenness, R.; Keeney, M. and Elmer, H.M. 1988 Fundamentals of Dairy Chemistry Van
Nostrand Reinhold Co., New York, U.S.A.
Fox, P.F. 1985 Developments in Dairy Chemistry Vol. 3 Applied Science, U.K.
Walstra, Pieter and Jenness, Robert 1984. Dairy Chemistry and Physics. John & Wiley and Sons, New
York.
Jenness, Robert, G. 1995. Hnadbook of Milk Composition. Academic Press, New York, U.S.A.
3. Chemistry of Milk Lipids 2 + 1
Milk lipids: classification, gross composition and physical properties; neutral and polar lipids and their role
in milk and milk products; fatty acids profile: composition, properties and factors affecting them;
unsaponifiable matter: composition with special reference to sterols and fat soluble vitamins and
caretenoids, chemistry, physiological functions and levels in milk; chemical properties: hydrolysis by
alkali, water and enzymes; hydrogenation and halogenation; transesterification and interesterification;
oxidation by chemical reagents; auto oxidation: definition, theories, induction period, secondary products
of auto oxidation, factors affecting, prevention and measurement; antioxidants-definition, types, reaction,
mechanism and estimation.
Practical
Determination of melting point/slip point, peroxide value, TBA value, carbonyl value, unsaponifiable
matter; estimation of total cholesterol by direct and indirect method; vitamin A, total phospholipids and
free fatty acids in ghee; preparation of fatty acid esters and their GLC analysis; estimation of antioxidants
(BHA); determination of CLA content in deshi ghee.
Suggested Readings
Fox, P. F. 1983 Development in Dairy Chemistry, Vol. 2, Applied Science Publishers, U. K.
Wong, N.P.; Jenness, R.; Keeney, M. and Elmer, H.M. 1988 Fundamentals of Dairy Chemistry Van
Nostrand Reinhold Co., New York, U.S.A
Walstra, P. and Jenness, R. 1984. Dairy Cemistry and Physics, John Wiley and Sons, New York, U.S.A.
Mathur,. M.P., Datta, Roy, D. and Dinakar, P. 1999. Text Book of Dairy Chemistry, 1CAR New Delhi.
Akoh, Casimir, C. and Min, D.B. 1997. Food Lipids: Chemistry, Nutrition and Biotechnology, Marcel
Dekker, New York, U.S.A.
4. Chemistry of Milk Proteins 2 + 1
27
Distribution and fractionation of different nitrogen fractions; nomenclature of milk proteins; major milk
proteins: caseins (acids and micellar), methods of isolation and heterogeneity; fractionation of casein;
physicochemical properties, glycosylation phosphorylation, amino acid composition; primary and
s<:condary structure of different fractions; casein models; alpha-lactalbumin and beta-lactoglobulin-
distribution, methods of isolation; whey protein concentrates and their functional properties; denaturation
of caseins and whey proteins as affected by change of temperature, pH and additives; casein-whey protein
interactions; genetic polymorphism and biosynthesis of milk proteins; minor milk proteins: proteose-
peptone, non-protein nitrogen constituents, immunoglobulins, lactotransferrin (lactoferrin), lipoprotein and
fat globule membrane proteins; milk enzymes: properties and their significance with particular reference to
lipases; phosphatases, catalase, peroxidase, xanthine oxidase, lysozyme, lactoperoxidase and galactosyl
transferase.
Practical
Estimation of different nitrogen fractions of milk by Kjeldahl and Folin methods; isolation of acid and
micellar casein; isolation of casein fractions by urea fractionation techniques; separation of amino acids
using thin layer/paper chromatography; isolation of alpha-lactalbumin and beta-lactoglobulin by
ammonium sulphate precipitation; polyacrylamide gel electrophoresis of milk proteins; estimation of milk
enzymes like catalase, lipase, alkaline phosphatase and xanthine oxidase; fractionation of milk proteins by
molecular sieving; estimation of hexoses, and free and bound sialic acid in casein.
Suggested Readings
Fox, P. F. and. MC Sweeney, P.L.H. 1998 Dairy Chemistry and Biochemistry, Blackie Academic
Professional Champman and Hall, London
Fox, P.F. 1992. Advanced Dairy Chemistry - I Proteins. Elsevier Applied Science, London. Walstra, P.
and. Jenness, R 1984. Dairy Chemistry and Physics, John Wiley & Sons, New York.
Mathur,. M.P., Datta, Roy, D. and Dinakar, P. 1999. Text Book of Dairy Chemistry, ICAR, New Delhi.
Fox, P. F. 1982. Development in Dairy Chemistry, Vol. 2, Applied Science Publishers, U. K.
Robert, G. Jensen, 1991. Handbook of Milk Composition. Academic Press, New York.
A2. OPTIO�AL COURSES
1. Chemistry of Milk Products 3 + 1
Heat stability of concentrated milk as affected by different process variables; milk constituents and
additives; physico-chemical changes taking place during manufacturing and storage of concentrated milk;
structure and physico-chemical properties of dried milk as affected by different process treatments;
stability of milk powder; physical properties of instant milk powder; role and mechanism of action of
stabilizers and emulsifiers in ice cream; distribution of fat globules and factors affecting it; creaming
phenomena; mechanism of churning; theory and metabolic pathways of fermentation; physico-chemical
characteristics; grading and standards of butter; genesis of flavour; aroma and texture in ghee; adulteration
of ghee and its detection; milk clotting enzymes from different sources (animal and plant); their isolation;
purification and action; changes taking place during manufacturing and ripening of cheese; chemical de-
fects in cheese.
Practical
Determination of lactose and sucrose in condensed milk and ice-cream; determination of heat stability of
milk and its concentrate; determination of moisture in skim milk powder/infant food by vacuum oven;
determination of fat in cream by Gerber and Rose-Gottlieb methods; determination of moisture, fat
28
(Gerber method), curd and salt in butter; determination of diacetyl and acetyl methyl carbinol in
butter/cultured products; determination of RM, Polenske value, iodine value, saponification value and
vitamin E in ghee; determination of nitrite, nitrate free amino acids and free fatty acids in cheese;
determination of rennet clotting time of milk.
Suggested Readings
Wong, N.P., Jeness, R., Keeney, M. and Elmer, H.M. 1988. Fundamentals of Dairy Chemistry, Van
Nostrand Reinhold Co., New York, U.S.A.
Walstra, P. and Jenness, R. 1984. Dairy Chemistry and Physics. John Wiley and Sons, New York, U.S.A.
Fox, P.F. 1985. Developments in Dairy Chemistry-3. Applied Science Publisher, U.K.
Law, B.A. 1997. Microbiology and Biochemistry of Cheese and fermented milks. Blackie Academic and
Professional, Chapman and Hall, 2nd Edn.
Gurr, M.I. 1981. Comparative aspects of Feeding Human and Artificial milk for infant feeding. J. Dairy
Res., 48, 519.
2. Food Chemistry 3 + 1
Forms of water in foods, water solute interactions, and food stability, solute mobility and food stability;
role of ice in the stability of food at sub-freezing temperatures, forms, swelling, gelatinization; food
applications and role of starch in bread making; modification of starches for industrial applications,
physico-chemical changes taking place during malting; functional properties of food proteins and their
modifications, denaturation of food proteins; enzymes & their application in food industry; physico-
chemical properties of food lipids and their modifications; mutual interactions of hydrocolloids and
interactions with proteins and lipids; role of hydrocolloids in different food preparations; indigenous and
synthetic food pigments; legal requirements for food colourants; flavour compounds of different foods and
flavour enhancers; changes taking place during fermentation; drying and roasting of chocolate and cocoa;
chemistry of tea manufacture; composition of coffee beans; physico-chemical changes during roasting of
coffee beans.
Practical
Determination of level of artificial sweeteners and crude fiber in food products; starch in flour by
polarization method; total amino acids and polyphenols in lemon juice; fat in grains; proteins in flour;
tannins in coffee/tea, caffeine content in coffee; HMF in honey, and visit to a food industry.
Suggested Readings
Fennema, O.R. 1985. Food Chemistry, Marcer Dekker Inc., New York
Belitz, H.D. and Grosch, W. 1987. Food Chemistry
Srinivas, D. and Alan Praf, 1997. Food Proteins and their Applications. Marcel Dekker Inc., New York.
Turker, G.A. and Woods, LFJ, 1995. Enzymes in Food Processing. Blackie Academic Professionals.
Williams, P.A. and Phillips, G.O. 2000. Gums and Stabilizers for the Food Industry. Royal Society of
Chemistry.
3. Research Techniques 3 + 1
Electrophoresis: principle and types, isoelectricfocussing; chromatography: column, TLC, GLC, HPLC,
gel-permeation, ion-exchange, affinity, spectrophotometry: UV, visible, IR and flame photometry;
potentiometry: principle, various electrodes; electrometric measurements of pH, buffers; radiotracer
technique: nuclear transformation, nuclear decay, measurement of radioactivity and safety precautions for
radioactive materials; membrane processes; ultracentrifugation.
29
Practical
Preparation of methyl esters of fatty acids of milk fat followed by their GLC separation and estimation;
TLC separation of amino acids; determination of Kav of a macromolecule using sephadex; preparation of
a buffer and measurement of its pH electro-metrically and using indicators; SDS gel electrophoresis and
molecular weight determination; determination of absorption spectra of BSA and demonstration of Beer's
law; determination of sodium and potassium by flame photometry; ultracentrifugal separation of milk and
preparation of micellar casein; ion-exchange chromatography separation of milk proteins.
Suggested Readings
Cooper, T.G. 1977 The Tools of Biochemistry, John Wiley & Sons, U.S.A.
Clark, J.M. and Switzer, R.L 1977 Experimental Biochemistry; W.H.Freeman & Co, U.S.A.
Stock, R. and C.B.F. 1974. Chromatographic methods; Rice, Chapman and Hall. U.K.
Frank, A. Settle 1997. Handbook of Instrumental Techniques for Analytical Chemistry. Prentice Hall,
PTR, Upper Saddle River, NJ 07458.
Nielsen, S. Suzanne 1994. Introduction to the Chemical Analysis of Foods, Jones and Barlett Publishers,
Boston, London.
4. Chemical Quality Assurance 2 + 1
Concept of quality assurance and quality control in relation to dairy industry; quality management systems
- ISO 9000; total quality management (TQM); hazard analysis of critical control points (HACCP); good
manufacturing practices (GMP); role of international organisations such as ISO; IDF; CAC; AOAC; WTO
and national organisations like BIS; CCFS; PF A and Agmark; significance of milk and milk products
order (MMPO) and APEDA (Agricultural and Processed Foods Export Development Authority) in dairy
industry; guidelines for setting up quality control laboratory; sampling of milk and milk products;
instrumentation in analysis of milk and milk products; chemical residues : pesticides; antibiotics; heavy
metals; radionuc1ides etc; in dairy products; dairy detergents and sanitizers; detection of adulterants in
milk and milk products; to check the calibration of milk testing glassware; preparation of standard
reagents; packaging materials in relation to quality assurance of dairy products.
Practical
Preparation of standard solutions and buffers; testing of available chlorine content in hypochlo-
rites/bleaching powder; determination of purity of common salt for butter and cheese making; detection of
common adulterants in milk and foreign fat/oil in ghee; checking the calibration of lactometers;
hydrometers; butyrometers; milk pipette and thermometer; qualitative colour tests to distinguish between
azo dyes and natural dyes in butter; detection of pesticide residues and antibiotics in milk.
Suggested Readings
IDF - Special Issue No. 9302. Quality Assurance (QA) and Good Lab. Practices (GLP) In Dairy
Laboratories.
IDF - Special Issue No. 9701 1997 Monograph on Residues and Contaminants in milk and milk products
Prevention of Food Adulteration Act (PFA) 1954 and PFA Rules. 1955 Official methods of A.O.A.C. (11th
and 15th editions)
ISI Handbook of Food Analysis S.P. 18 (Part II) Dairy Products. 1981 ISI Specifications (concerned) (ISI)
Ralph Early, Guide to Quality Management System for Food Industry; Heinz Bullworth, Establishment and
Implementation of HACCP Personal Hygiene Practices.
5. Advances in Chemistry of Milk Lipids 3 + 0
30
Origin, composition; structure and physical chemistry of milk fat globule and membrane - comparative
aspects of milk lipids from different species such as human, bovine, buffalo, sheep, goat, pig and camel;
lipolytic enzymes from different species including human bile salts; stimulated lipase and esterases;
biosynthesis of fatty acids; glycerol; neutral lipids; phospholipids; sphingolipids; cholesterol; essential fatty
acids; prostaglandins and flavour compounds; conjugated linoleic acids - different isomers; factors
affecting their levels in dairy products and their signficance; chemistry of oxygen in relation to
autoxidation of milk fat including effect of milk components and enviornmental factors; thermal oxidation;
chemical and biological properties of heated and oxidised fats; significance of milk lipids in human health;
role of milk lipids in consumer acceptance of dairy products; fat replacers in dairy products.
Suggested Readings:
Wong,N.P.; Jenness, R.; Keeney, M. and Elmer H. M. 1988 Fundamental of Dairy Chemistry 3rd Ed. Van
Nostrand; Reinhold Company, New York.
Walstra, P. and Jenness, R. 1984 Dairy Chemistry and Physics, John Wiley & Sons, New York
Fox, P. F. and McSwamy, PLH 1997 Dairy Chemistry and Biochemistry London: Blackie
Fox, P. F. 1995 Advanced Dairy Chemistry Vol. 2 Lipids 2nd Ed. Chapman & Hall, London
Gurr, M. I. 1992 Role of Fats in Food and Nutrition, Elsevier Applied Science Publishers, New York
Bindra, J. S. and Ranjana Bindra 1977 Prestoglandins Synthesis, Academic Press, New York.
6. Advances in Chemistry of Milk Proteins 3 + 0
Biosynthesis of milk proteins, proteins and lipoproteins of milk fat globule membrane (MFGM); amino
acid sequence of caseins, structure - function relationship of casein and whey protein; association -
dissociation equilibria; physical, chemical and enzymatic modification of milk proteins and their functional
characteristics; mechanism of action and biological role of specific and non-specific antimicrobial factors
in milk - immunoglobulins, lactoferrin, lactoperoxidase and lysozyme; milk derived bioactive peptides -
their properties; significance and application; bitter peptides in cheese;; growth factors in milk; transgenic
milk proteins and their significance; therapeutic and allergy aspects of milk proteins; protein films and
coatings their properties and applications.
Suggested Readings
Fox, P. F. 1995 Advanced Dairy Chemistry, Vol. 1. (Proteins)
Damodaran, S. & A. Paraf 1997 Food Proteins and their applications
Welch; R.A.S.; D.J.W. Burns; S. R. Davis 1997 Milk Composition, production and biotechnology.
Popay, A. I., C.G. Prosser 1997 Biotech in Agric. Series No. 18, CAB International Hans Visser; 1992
Protein - Interactions. VCS, New York
Proceedings of IDF Seminar on Indigenous antimicrobial agents of milk - Recent developments; Uppasala,
Sweden, 1993
Cregg, B. Fields 1997 Solid Phase Peptide Synthesis; In: Methods in Enzymology, Volume 289.
B. SUPPORTI�G COURSES
1. Bio-organic Chemistry and Enzymology 3 + 1
Chemistry of carbohydrates, lipids, amino acids, purines and pyrimidines; chemical synthesis of
polypeptides and polynucleotides; structure of proteins, nucleic acids and polysaccharides in solution;
enzymes: definition, classification, kinetics of enzyme catalyzed reactions, Michaels-Menten equation, Km
and Vmax, enzyme inhibition; factors affecting enzyme activity; principles of isolation and purification of
enzymes; enzyme activity unit, substrate specificity; identification of functional groups essential for
enzyme activity; active site; mechanisms of enzyme action; regulatory enzymes, allosteric regulation,
isoenzymes.
31
Practical
Estimation of glucose content of blood serum by titrimetric method; glucose with glucose oxidase;
cholesterol in blood; fructose content in seminal plasma; protein by Biuret method; protein by Lowry's
method; triacylglycerols; determination of the specific activity of alkaline phosphates of bovine seminal
plasma; effect of enzyme concentration on the activity of alkaline phosphatase; determination of pH optima
of an enzyme; determination of temperature optima of an enzyme; determination of Km and Vmax of an
enzyme.
Suggested Readings
Nelson, D.I. and Cox, M.M. 2000. Lehninger Principles of Biochemistry, 3rd Ed. Macmillan Worth
Publishers, USA.
Stryer, L. 1988. Biochemistry, 3rd ed. W.H. Freemand and Co., New York.
Zubay, G. 1988. Biochemistry 2nd ed. MacMillan Publishing Co., New York, CollierMcMilian Publishers,
London.
Moran, L.A.; Scrimgeour, K.G.; Horton, H.R.; Gchs, R.S. and Rawn, J.D. 1994. Biochemistry, 2nd Ed.
Publisher-Neil Patterson, Prentice Hall Inc., Englewood Cliffs, N.J.
Murray, R.K.; Granner, O.K.; Mayes, P.A. and Rodwell, V.W. 2000. Harper's Biochemistry 25th Ed.
Publisher-Appleton & Lange/Prentice Hall International, London.
2. Intermediary Metabolism 3 + 0
Survey of intermediary metabolism; principles of bioenergetics, glycolysis; citric acid cycle; electron
transport and oxidative phosphorylation; pentose phosphate pathway, glyoxylate cycle, glycogenolysis; B-
oxidation of fatty acids; oxidative degradation of amino acids; determination of nucleotides;
gluconeogenesis, glycogen synthesis; biosynthesis of carbohydrates, lipids, amino acids and nucleotides;
photosynthesis; nitrogen and sulphur cycles; urea cycle; transport across biological membranes; the
energetics of active transport; organization of membrane transport systems in animals tissues; genetic
evidence for transport systems.
Suggested Readings
Moran, L.A.; Scrimgeour, K.G.; Horton, H.R.; Gchs, R.S. and Rawn, J.D. 1994. Biochemistry, 2nd ed.
Publisher-Neil Patterson, Prentice Hall Inc., Englewood Cliffs, N.J.
Murray, R.K.; Granner, D.K.; Mayes, P.A. and Rodwell, V.W. 2000. Harper's Biochemistry 25th Ed.
Publisher-Appleton & Lange/Prentice Hall International, London.
NeJson, D.L. and Cox, M.M. 2000 . Lehninger Principles of Biochemistry, 3rd ed. Macmillan Worth
Publishers, USA.
Stryer, L. 1988. Biochemistry, 3rd ed. W.H. Freeman and Co., New York.
Zubay, G. 1988. Biochemistry 2nd ed. MacMillan Publishing Co., New York, .Collier McMillan
Publishers, London.
3. Dairy & Food Packaging 2 + 1
Status of current packaging; critical review of the existing knowledge in packaging of products; adhesives,
graphics and labeling used in food packaging; protective packaging of foods; packaging of food products
sensitive to oxygen, light, moisture; special problems in canned foods; packaging of convenience foods;
fruits and vegetables; packaging requirements of fresh fruits and vegetables; packaging of fruit juices; fats
and oils: criteria for selection of proper packaging based on the shelf life desired; packaging of spices;
packaging of meat and poultry: packaging and transportation of fish and other sea-foods; new food
packaging processes; retort pouch technology, microwave packages, bio-degradable packages, edible
packages; industrial packaging: unitizing, palletizing, containerizing, stacking and materials handling;
32
distribution systems for packaged foods including prevention of shock damage to articles during transpor-
tation; safety aspects of packaging materials; sources of toxic materials and migration of toxins into food
materials.
Practical
Determination of thickness, GSM, grease resistance, bursting strength, tearing resistance, WVTR, puncture
resistance and edge crush resistance of packaging materials; estimation of shelf life of leafy vegetables and
seasonal fresh fruits like apples; litchi; and oranges; packaging of turmeric powder and ground red chilli
powder, testing of packaging materials for quality assurance; vacuum packaging of dairy products;
packaging of food products using retort pouch technology; packaging of food products employing
microwave technique.
Suggested Readings
Malhlouthi, M. 1994 Food packaging and preservation, B1ackie Academic & Professional, Glasgow.
Carol, F; Steinhart, M., Ellin Doyle; Barbara A. and Cochrane, 1995 Food Safety, Marcel Dekker Inc.,
Madison, Wisconsin.
Frank A., Paine Heather, Y. Paine 1983 A Handbook of Food Packaging, Leonard Hill, Glasgow
Sacharow, S. and Griffin, R.C. 1970 Food packaging. The A VI Publication, Westport, CT. USA.
4. Microbiology of Processed Foods 2 + 1
Microbial ecology of foods; main concerns; ecology of prepared foods; mechanisms of stress induced
injury to microorganisms in processed milk; repair of injured organisms; enumeration of stressed cells;
predictive modeling for food spoilage and safety; low temperature food preservation; thermal processes for
shelf stable products; recent concepts in irradiation technology; safety aspects and social acceptance; new
methods for controlling spoilage of foods; modified atmosphere packaging and shelf life of processed
foods; microbiological safety considerations; intermediate moisture foods and hurdle concept; new
prospects and problems in fermented foods; potential applictions of fermentation processes, genetically
engineered products, and ecosystem: effects of technological changes.
Practical
Production of antimicrobial factors; application of bacterial consortia in fermented food application of
hurdle concept for enhanced shelf stability of processed foods; induction of bacterial cell injury and
recovery of injured cells; effect of modified atmosphere packaging on quality and shelf life of processed
foods; production of alcoholic beverage by whey fermentation; extending shelf life of foods by food grade
bio-preservatives; development of predictive equation and its validation for food safety; assessment of
pollution in food processing environment and biodegradation of xenobiotics in foods.
Suggested Readings
James M. Jay 1987 Modem Food Microbiology, 3rd Edition, CBS Publishers & Distributors, Shahdra,
Delhi.
Susan, K. Harlander and Labuza, T. P. 1986 Biotechnology in Food Processing, Noyes Publications, New
Jersey, USA
Hui, Y.H. and George G. Khachatourians 1995 Food Biotechnology: Microorganisms, VCH Publishers
Inc, New York
Robinson, R.K. 1998 Developments in Food Microbiology Vol. 1, 2 and 3, Elsevier Applied Sciences,
London and New York.
33
5. Statistics for Industrial Applications 2 + 1
Statistical methods in industrial applications; analysis of variance; transformations; partial and multiple
correlation and regression; Ranking techniques; introduction to discriminant analysis; statistical basis for
drawing scientific inferences from experimental data; principles of experimental design-industrial
experimentation; basic designs-CRD; RED & LSD; missing plot technique; factorial experiments-main
effects and interactions; 2n series and mixed factorial experiments; experimental designs in sensory
evaluation; introduction to statistical quality control; control charts for variables; mean and range charts;
statistical basis; rational sub-group; control charts for attributes 'np'; 'p' and 'c' charts; fundamental
concepts of acceptance sampling plans; single; double and sequential sampling plans; use of sampling
inspection tables for selection of single and double sampling plans; introduction to sampling techniques
and their application to consumer preference studies.
Practical
Analysis of variance-one way and two way classification; partial and multiple correlation and regression;
rank correlation and coefficient of concordance; analysis of industrial experiments Use of CRD; RED and
LSD; missing plot technique; factorial experiments - 22 and 23; mixed factorial experiments; control
charts for variables; control charts for attributes; single sampling plan- OC and AOQ curves; sequential
sampling plan; use of sampling inspection tables; and different methods of selecting samples.
Suggested Readings
Snedecor, G. W. and Cochran, W.G. 1968 Statistical Methods Oxford and IBH Publishing Co., New Delhi
Cochran, W. G. 1972 Sampling Techniques. Wiley Eastern Pvt. Ltd., New York
Cochran, W. G. and G.M. Cox 1962 Experimental Designs Asia Publishing House, Bombay Grant and
Leavenworth 1972 Statistical Quality Control, McGraw Hill, New Delhi
Indian Standards Institute 1986 New Delhi Handbook on Statistical Quality Control.
6. Computer Software Packages for Statistical Analysis 2 + 1
General data analysis requirements in dairy research; introduction to statistical and other standard software
packages (SYSTA T; SPSS; MS-Excel); data preparation and job control commands for statistical analysis
of data pertaining to t-test; Chi-square test; analysis of variance (ANOY A); basic experimental designs -
CRD; RED & LSD; factorial designs; partial and multiple correlation and regression; discriminant analysis;
linear programming; (using LINDO/ LINGO) software packages; least-squares analysis.
Practical
Statistical software packages and their operations; data preparation and data generation; import and export
of data from spreadsheet and database packages; application of software packages to the problems related
to: tests of significance (t; Chi-square and F-test); analysis of variance (ANOY A); partial and multiple
correlation and regression; discriminant analysis; linear programming problem; and least-squares analysis.
Suggested Readings
Snedecor, G. W. and Cochran, W.G. 1967. Statistical Methods; Oxford and IBH Publishing Co., New
Delhi
Levin, Richard I. and Rubin David, S. 1994. Statistics for Management, Prentice Hall of India, New Delhi.
Amble, Y.N. 1975 Statistical Methods in Animal Science, Indian Society of Agricultural Statistics, ICAR,
New Delhi.
Singh, R.K. and Chaudhary, B.D. 1979. Biometrical Methods in Quantitative Genetic Analysis. Genetic
Analysis, Kalyani Publishers, Ludhiana.
34
Swarup, Kanti, Gupta., P.K. and Man Mohan 1997. Operations Research. Sultan Chand and Sons, New
Delhi.
7. Research Documentation 1 + 1
Library as information centre; information technology; CD ROM data search; abstracts and abstracting
techniques; the concept of documentation; international network/Indian scene; information retrieval
systems; MIS; science citation; web internet search; presentation of results of research; different kinds of
scientific writings: writing of scientific reports, thesis, research papers etc; oral and poster presentation of a
paper in conferences/symposia; standard abbreviations used in proof reading; printing technology and
corrections of manuscripts.
Practical
Different schemes of classification & various cataloguing codes for arranging documents; use of
catalogues & classification; types of libraries & standard sources of information; familiarity in use of
standard sources of information; exercise in abstracting of research articles; demonstration of
photography; photocopying; printing; preparation and handling of audio-visual aids; preparation of
multimedia; exercise in proof corrections; research paper writing & interpretation; CD-ROM search; and
Internet search.
Suggested Readings
Balaguru, T. 1996. Managem{)ut Information Systems for Agricultural Research, NAARM, Hyderabad.
Chhotey Lal 1998. Agricultural Libraries & Information Systems: A Handbook for Users. R.K. Techno
Science Agency, New Delhi.
Guha, B. 1987. Documentation & Information. World Press Pvt. Ltd., Kolkatta
Kau1a, P.N. etc. 1996. International & Comparative Librarianship and Information Systems. B.R.
Publishing Corpn., Delhi.
Venkatasubramanian, V. 1999. Introduction to Research Methodology in Agricultural & Biologial
Sciences. New Century Book House, Chennai.
Sharma, P.S.K. 1987 Library & Society. Ess Ess Publication, New Delhi. CBF Style Manual Published by
Council of Biology, Maryland.
35
DAIRY E�GI�EERI�G
AI. CORE COURSES
1. Dairy and Food Engineering -I 2 + 1
Engineering properties of dairy and food materials and their significance in equipment design; processing
and handling of dairy and food products; concept of rheology; ideal elastic, plastic and viscous behaviour;
viscoelasticity; rheological models and constitutive equations; Maxwell model; Kelvin model and Burgers
model; viscoelastic characterisation of materials; stress-strain behaviour; creep; stress relaxation; non-
Newtonian fluids and viscometry; rheology and texture of food materials; methods of texture evaluation:
subjective and objective measurements, mechanical tests, firmness, hardness, dynamic hardness; objective
methods of measuring texture: Kramer shear stress, GFT, OTMS, Instron universal testing machine;
rheological properties of dairy products; strength of food materials; aerodynamic and hydrodynamic
characteristics; drag coefficient; terminal velocity and Reynold's number;. application of aerodynamic
properties to the separation; pneumatic handling and conveying of food products; material and energy bal-
ance; evaporation and dehydration of foods; design of single and multi - effect evaporators; design of spray
dryer and its components; separation and recovery of dried product; design of recovery system; selection
and design of auxiliary equipments; freezing of foods; freeze concentration and drying; freezing point
curves; phase diagrams; methods of freeze concentration; design problems.
Practical
Working of Instron universal testing machine; stress strain behaviour analysis of dairy and food products
using Instron testing machine; rheological studies by stress relaxation methods; creep behaviour of dairy
and food products; determination of thermo-physical properties of dairy and food products; determination
of parameters of Newtonian and non-Newtonian food products by Capillary tube viscometer; Rotational
viscometer and falling ball viscometer; design problems on evaporators; dryers; freeze drying etc.
Suggested Readings
Heldman, D.R. and Singh, R. P. 1984 Food Process Engineering The A VI Publishing Company
Mohsenin, N. N. 1986 Physical Properties of Plant and Animal Materials Gorden and Breach Science
Publishers New York
Rao, M.A. and Rizvi, S.S.H. 1986 Engineering Properties of Foods Marcel Dekker Inc., New York.
Watson, E.L. and Harper, lC.1989. Elements of food engineering. AVI Publishing Company, New York.
Charm, S.E. 1971. The Fundamental of Food Engineering. AVI Publishing Company, New York.
2. Dairy and Food Engineering - II 2 + 1
Water activity and states: a thermodynamic quantity, water sorption isotherms, hysteresis, theories of
sorption hysteresis, water activity measurement methods, water binding, control of water activity and
moisture, principles of IMF and their application; permeability and shelf-life: theoretical considerations,
permeability to gases and vapours, measurement methods, permeability of multilayer materials,
permeability in relation to packaging requirements of food products; calculation of shelf life and
requirements for packaging; deteriorative reactions;_ accelerated testing; relationship between transport
properties of the package and shelf life of packaged products; simulation of product-package-environment
interaction, shelf life simulation for moisture; oxygen and light sensitive products; theory of ultra filtration
and reverse osmosis; selection and types of membranes and properties; concentration polarization;
mathematical description of flow through membrane; application and use in dairy industry; microwave
energy absorption: physical parameters in microwave heating processes, heat transfer phenomena, equip-
ment and application in dairy and food industry.
36
Practical
Determination of water activity of foods; establishment of moisture sorption isotherm; determination of gas
transmission rates of packaging film; determination of water vapour permeability of packages; shelf life
calculation for moisture sensitive and oxygen sensitive foods; design problem on packaging systems.
Suggested Readings
Rockland, L.B.and George, F. Stewart. 1991. Water activity: Influence on Food Quality Romeo, T. Toledo.
1997. Fundamentals of Food Process Engineering
Gordon, L.Robertson. 1992. Food Packaging (Principles and Practices).
Cheryan, M. 1998. Ultrafiltration and Microfiltration Handbook.
Duckworth, R. 1975. Water Relations in Foods. Academic Press, Inc., New York
3. Heat Transfer 2 + 1
Conduction: heat transfer through rectangular fin, actual solution, effectiveness, efficiency, approximate
solution, design of profile area, triangular cylindrical and parabolic fins; two-dimensional steady-state heat
conduction, analytical and graphical solution, numerical analysis, Unsteady-state heat conduction;
graphical; analytical and numerical methods of solving unsteady state heat transfer problems, periodic heat
flow; hydrodynamic and thermal boundary layer: equation of motion for hydrodynamic boundary layer;
energy equation of thermal boundary layer; velocity distribution; temperature distribution; local heat
transfer coefficient, Nusselt number, velocity distribution in the entrance region of a tube, turbulent flow
conventive heat transfer: empirical relations in convection; the Stanton number, general equation for forced
convection from dimensional analysis, flow in a tube; parallel to a plate, film-wise condensation on vertical
surface, condensation heat transfer coefficients of a film, equations for condensation on vertical surfaces;
boiling liquids; parallel flow; counter flow; cross flow; plate heat exchangers; hydro-mechanical design;
effectiveness; number of transfer units.
Practical
To prepare and calibrate a thermocouple; to study construction, working of microwave oven and its heating
profiles; to find out thermal conductivity of dairy products/insulating powder; to study the temperature
distribution in water heated in a steam jacketed kettle (under unstirred conditions); to study the temperature
distribution in milk heating in a steam jacketed kettle (under unstirred conditions); determination of film
heat transfer co-efficient during khoa manufacturing in a steam jacketed kettle; studies on parallel flow heat
exchanger; studies on counter flow heat exchanger; determination of film heat transfer co-efficient during
manufacturing of ghee from cream; boiling heat transfer co-efficient of water; boiling heat transfer
coefficient of ghee.
Suggested Readings
Kreith, F. 1965 Principles of Heat Transfer International Text book Company, Scranton, Pennsylvania.
Eckert, E.R.G. and Drake, R.M. 1959 Heat and Mass Transfer McGraw-Hill Book Co. Inc., New York.
Jacob, M. and Hawkins, G. A. 1957 Elements of Heat Transfer John Wiley & Sons Inc., New York.
Bird, R. B. Stewart, W. E. and Lightfoot, E. N. 1994 Transport Phenomena John Wiley and Sons Inc., New
York
Gupta, C. P. and Rajendra Prakash 1986 Heat Transfer Nem Chand & Bros, Roorkee, U.P.
4. Bio-thermal Process Engineering 2 + 1
Introduction to biochemical engineering: kinetics of substrate utilization; product yield and biomass
production in cell cultures; gas liquid mass transfer in microbial systems; design and analysis of
37
fermentation vessels; residence time distribution; introduction to thermal processing; sterilization
classification; UHT systems and recent advances; factors affecting spoilage of different types of food
products and design of thermal process; aseptic packaging systems and conditions; survivor curve; thermal
death curve; arrheneous curve; techniques for determination of heat resistance of microorganisms; analysis
of thermal resistance data; processing in containers; process time; lethality; general formula for evaluation
of heating and cooling process; broken heating curve; design of batch and continuous sterilization cycles in
vat; inter-relationship between batch and continuous reactors; design calculations.
Practical
Design calculation on sizing of Fermenter, mechanical design of fermenter vessel, Agitation system design
for the fermenter, Design of cell separation equipment, Pressure and temperature profile in hydrostatic
sterilizer, Assessment of UHT-heat treatment methods
Suggested Readings
Stumbo 1965 Thermobacteriology in Food Processing Academic Press, New York
Olson, J. E. Bailey and David F. Ollis 1977 Biochemical Engineering Fundamentals; McGrawHill Book
Co. Inc., New York
Blanch, H. W. and Clark, D. S. 1996. Biochemical Engineering. Marcel Dekker Inc., New York.
Larousse, Jean and Brown, B. E. 1997. Food Canning Technology, Wiley- YCH, USA.
Pauline,M. Doran. Bioprocess Engineering Principles, Academic Press.
A2. OPTIO�AL COURSES
1. Transport Phenomena 2 + 0
Introduction to transport phenomena-molecular transport mechanism, transport properties and their
proportionality constants in momentum, energy and mass transfer;, steady state equations; momentum
transport equations for Newtonian and non-Newtonian fluids: continuity equation in different co-ordinates,
equations of motion, Navier-Stokes equations and their application in viscous fluids flow between parallel
plates and through pipes; turbulent transport mechanism mathematical analysis, eddy viscosity and eddy
diffusivity; velocity; temperature and concentration distribution; time smoothing equations; interphase
transport in isothermal system - friction factors for various geometrics; mass transfer:- Fick's law of
diffusion; diffusion of gases and liquids through solids; equimodal diffusion; Isothermal evaporation of
water into air; mass transfer coefficient; dimensional analysis - Buckingham Pi-theorem and matrix
l)1ethod; application to transport phenomena; analysis among mass; heat and momentum transfer;
Reynolds; Colburn and Martinelli analogies; boundary layer concept; theory and exact solutions for heat;
mass and momentum transfer.
Suggested Readings
Bird, R.B. 1994 Transport Phenomena John Wiley & Sons Inc., New York.
Yuan, S. W. 1969 Foundations of Fluid Mechanics Prentice Hall of India Pvt. Ltd., New Delhi.
Treybal, R.E. 1968 Mass Transfer Operations McGraw Hill Kigakusha Ltd., New Delhi.
2. Instrumentation and Process Control 2 + 1
Instrument terminology and performance system accuracy; flow sheet symbols; instrument evaluation:
electrical, mechanical, magnetic and optical transducers for measurement of process variables like
temperature, pressure, flow, level, consistency and humidity; indicating and recording devices: direct
acting and servo operated systems; digital indicators, strip and circular chart recorders, electronic data
loggers; principles of automatic process control: process characteristics; controller characteristics; closed
loop system; pneumatic and electric controllers; final controlling elements : control valves; valve sizing;
38
electronic actuators; motor drives and controls; introduction to programmable logic controllers (PLCs):
internal structure; inter facing with sensors and actuators; binary logic diagrams and ladder diagrams;
choosing a PLC system.
Practical
Study of various transducers for measurement of pressure, flow, level, humidity; temperature; study the
controller and recorder of pasteurizer; the working of controllers in constant temperature water baths; to
make ladder diagrams and flow sheet diagrams for control logics; to programme a PLC; computer interface
of a PLC.
Suggested Readings
Douglas M. Considine 1974 Process Instruments and Controls. McGraw Hill Book Co. New York
Douglas M. Considine 1964 Handbook of Applied Instrumentation. McGraw Hill Book Co. New York
Beta G. Liptak 1995 Process Measurement and Analysis Butterworth-Heinmann Ltd. Oxford, U.K.
Kempe's Engineers Yearbook 1996 Miller Freeman Information Services, U. K.
Eckman, D.P., 1972. Automatic Process Control, Wiley Eastern Private Ltd., N.D.
3. Design of Process Equipment 2 + 1
Design of vessels: codes and regulations, design for pressure and temperature, loading; allowable stresses;
minimum thickness after forming; corrosion mechanism; corrosion control; design for internal and external
pressure; cylindrical and spherical shells; formed heads; reinforcement openings; fabrication requirements;
inspection; tests and non-destructive examination pressure tests; design and stress evaluation; design of
milk storage tank; horizontal and vertical silos; insulated and un-insulated; process plant piping: codes and
regulations; testing; fabrication requirements; overall economic and safety considerations; heat exchangers:
shell and tube heat exchangers; construction codes; general design considerations; U-tube heat exchangers;
double-pipe exchanger; scraped surface exchanger; spiral tube exchangers; joints; welded tube joints;
baffers and tube bundles; tube sheet bundle; by- passing longitudinal bundles; double tube sheet
construction; clad tube sheets; plate type exchangers; air cooled heat exchangers; heat exchanger costs;
heat transfer coefficients; tank coils; external coils and pipe line tracers; jacketed vessels.
Practical
Design of short, intermediate length and long cylindrical vessels; design of spherical vessels; design of
shell and tube; double pipe, scraped surface and spiral tube heat exchangers; design of jacketed vessels -
insulation thickness; corrosion allowance
Suggested Readings
Hesse, C. R. and Ruston, J.H. 1964 Process Equipment Design Affiliated East-West Press Pvt. Ltd., New
Delhi
Kern, D.Q. 1965 Process Heat Transfer McGraw-Hill, Kogakusha Ltd., New Delhi
Foust, A.S. and others 1960 Principles of Unit Operations John Wiley and Sons Inc; New York.
Evans, F.L., 1974, Equipment Design Hand-book, Vol. 2, Gulf Publishing Houston, Texas.
McCabe, W.L.; Smith, J.C., Harriott, P., 1993, McGraw Hill Inc., International Edition.
4. Computational Method & Simulation in Dairy and Food Engineering 2 + 0
Taylor's series expansion in development of numerical differentiation; numerical differentiation
procedures: forward difference, backward difference, central difference; numerical integration :
trapezoidal rule, Simpson's rule, improper integrals, Gauss-Legrendre Quadretuse method; numerical
39
methods to solve ordinary differential equations: Euler method, improved Guier method, Runge-Kutta
method, Adam's P-c method, initial value problems; numerical solution of partial differential equation:
explicit method, implicit method, simulation concept, simulation methods and their limitations, statistical
and theoretical models; problem formulation and development of models; solution and validation of
models; data collection; processing and analysis; basic modeling problems on unit operations involved in
dairy and food processing.
Suggested Readings
Krishnamurthy, E. V. and Sen, S.K. 1976 Computer based Numerical Algorithms
Franks, Roger, G. E., 1972 Modeling and Simulation in Chemical Engineering WilleyInterscience, New
York
Reddy, R.N. and Ziegler, C.A. 1992 FORTRAN-77 with Applications for Scientists and Engineers
Maisel, Herbert and Gougnoli 1972. Simnulation of Discrete Stochastic Systems., SRA Publisher
Kobayashi, H. 1978. Modeling and Analysis: An introduction to system performance Evaluation
Methodology. Addision-Wesley Publishing Company, Canada.
5. Environmental Engineering 2 + 0
Waste water sources characteristics standards for disposal of dairy water, physical, chemical and biological
characteristics of waste water; measurement of organic content in waste water; physical unit operations in
waste water treatment - screening; racks, mixing, flocculation, sedimentation, floatation, elutriation,
vacuum filtration, incineration; chemical unit operations in waste water treatment - reaction kinetics;
chemical precipitation, aeration and gas transfer process, rate of gas transfer, adsorption, disinfection;
biological unit operations - aerobic and anaerobic cycles; kinetics of biological growth, application of
kinetics to treatment systems, aerobic waste treatment; aerobic and anaerobic waste treatment; cooling load
estimation; air duct design and room air distribution; air conditioning systems; clean-room air conditioning;
important pollutants of air; properties of particulate matter and air pollution control methods. A case study -
STP/incineration plants.
Suggested Readings
Richard A Conway & Richard D. Ross 1980 Hand Book of Industrial Waste Disposal Van Nortrand
Reinhold Company, New York
Metcalf & Eddy 1984 Waste Water Engineering Treatment Disposal and Rense McGraw Hill Publication
Lewis & Athony 1976 Industrial Air Pollution Control Equipment for Particulate C.R.C. Press, Ohio
Threkeld 1970 Thermal Environmental Engineering Prentice-Hall Inc.
6. Dairy Process Engineering 2 + 1
Hygienic design concepts; sanitary pipes and fittings; milk pumps; milking machines; bulk milk coolers;
milk collecting and chilling centres; milk tanks; stirrers and mixers; milk reception equipment;
pasteurizers; sterilizers and treatment by irradiation; C1P system; corrosion process and their control;
centrifugation; separation; cyclone separators; homogenizers; ultrafiltration; reverse osmosis and
electrodialysis; equipment for cheese, ice-cream, butter manufacture; special milk products viz, casein,
whey and lactose etc; lndigenous milk product processing, batch and continuous manufacturing systems;
packaging and filling machines; milk evaporators; multiple effect evaporators; thermo-compressors and
MVR systems; roller dryer and spray dryer; spray dryer controls; fludization characteristics of gas-solid
fluidized systems; fluidized bed dryers.
Practical
Identification of hygienic characteristics of pipes & fittings; technical specifications of milking and storage
40
equipments; equipment for chilling & pasteurization; features of centrifuges; ice cream freezers & packing
machine; cheese vat; milking machine; press & packing machine; butter manufacturing equipments;
different types of evaporators;different types of dryers; design of dairy plants; design of milk collecting and
chilling unit.
Suggested Readings
Kessler, H.G. 1981 Food Engineering and Dairy Technology; Verlag A; Kessler P.O. Box 1721, 0-8050
Freising (F.R. Germany)
Ahmad Tufail 1997 Dairy Plant Engineering and Management, Kitab Mahal, Allahabad
Newcomer, J.L. 1981 Preventive Maintenance Manual for Dairy Industry.
4. Industrial Engineering & Management 3 + 0
Industrial evolution in India: evolution of modem industry, development of public enterprises; Industrial
Management: Organization, organization theories and structures, organization goals, planning and
effectiveness, management as a system in an organization, functions of management, scientific
management; Production Planning and Control: Introduction, PPC as a management function; objectives of
PPC; product design and development; demand analysis and forecasting; resource requirement planning ;
production planning, production scheduling; dispatching and follow up; material control. Material
Handling: Definition, main objectives; factors affecting material handling systems, types of material
handling equipments; material procurement and material storage; plant maintenance: Different system of
maintenance; elements of preventive maintenance, economic aspect of maintenance; Quality Control &
Inspection: Benefits of Q.c., Principles, function~ and objectives of Q.C., difference between Q.C. and
inspection; inspection procedures; Inventory Control: Necessity and advantages of inventory control; types
of inventory, procurement and consumption cycle; economic order quantity (EOQ) optimum safety stock,
techniques of inventory; Projectology: Project formulation and implementation strategies; monitoring and
control of projects, project evaluation-benefit cost analysis; Management Information Systems:
Significance of information as a corporate resource, identification, collection, storage and retrieval of
information, ferequency of reporting and updating, introduction to decision support systems; Human
resource development: Human resource planning- people, profit and productivity, staffing, recruitment
policy, training and development programmes, motivation, incentive and promotion protocols, collective
decision making, trade unions, and collective bargaining, wages and salary administration.
Suggested Readings
Ahuja, K.K. (1993) Industrial Management, Khanna Publishers, 3rd Edition.
Chauhan, A.S. & Vaishwanar, R.S. (1999) Management of Systems. Jain Brothers. 3rd Edition. Sharma,
S.C. 1999. Management of systems, Khanna Publishers, 5th Edition.
Dr. Kumar, S. 1997. Industrial engineering and management of manufacturing systems, Satya Parkash 1st
Edition.
Nauhria, R.N. & Parkash, 1998. Management of Systems, Dhanpat Rai & Co., 2nd Edition.
8. Refrigeration & Engineering 2 + 1
Methods of refrigeration: Ice and dry ice refrigeration, evaporative and steam jet refrigeration, refrigeration
by expansion of air, refrigeration by throttling of gas and by using liquid gases, vapour refrigeration
system, absorption refrigeration systems, heat pump.; Non-conventional Refrigeration Systems: Thermo-
electric refrigeration, vortex-tube, refrigerant-mixtures, cooling by adiabatic demagnetisation; Design
elements of Refrigeration Equipments: Design elements of compressor, condenser, evaporator, cooling
tower, spray pond; balancing of different components of the system; Design of cold storage and air
conditioning systems: types of loads in cold storage and their calculations, design of cold storage for food
products, construction of cold storage, equipment selection, insulating materials, vapour barriers, care and
maintenance of cold storage.
41
Practical
To study the working of absorption refrigeration plant; the working of heat pump; to design refrigeration
equipments for a milk chilling center and to balance the different components; design a cooling tower and
spray pond for a small dairy plant; design problems on cold storage for different food products; visit to
food cold storage and non-conventional refrigeration plants.
Suggested Readings
Domkundwar, S. 1980. A Course in Refrigeration and Air Conditioning, Dhanpat Rai & Sons, Delhi.
Richard C. Jordan and Gayle B Priester, 1965 Refrigeration and air conditioning (2nd Edition), Prentice
Hall of India (Private) Ltd., New Delhi.
Kadambi, V. and Hutchinson, F.W. 1968. Refrigeration, Air Conditioning and Environmental Control in
India. 1st Edition. Prentice Hall of India Private Ltd., New Delhi.
Arora, S.C., 1973. A Course in Refrigeration and Air Conditioning, Dhanpat Rai & Sons, Delhi.
Burgess H. Jenning and Samuel R. Lewis, 1963. Air Conditioning and Refrigeration. International Text
Book Company, Scranton, Pennsylvania.
B. SUPPORTI�G COURSES
1. Technology of Milk and Milk Products 1 + 1
Market milk industry in India and abroad; principles and practices of production of high quality milk;
cleaning and sanitization of dairy equipments; manufacturing process of cream, butter and ghee; legal
standards; basic principles and manufacturing processes for cheese; paneer and chhana; different types of
cheese and their classifications; ingredients and constituents of ice cream mix preparation, freezing point
determination; manufacturing process; basic principles; manufacturing processes of concentrated and dried
milk, defects in milk and their control.
Practical
Market milk reception; pasteurization and filling; preparation of butter, cheese, paneer, Ice cream,
concentrated milk and dried milk.
Suggested Readings
Allen, H. Vernon and Southerland, Jane P., 1994. Milk and Milk Product Technology, Chemistry and
Microbiology. Chapman and Hall.
Walstra, P. and Robertzenis 1994. Dairy Chemistry and Physics. John Wiley and Sons. De, Sukumar, 1980.
Outlines of Dairy Technology. Oxford University Press.
Walstra, P., Geurts, T. J., Noomen, A., Jellema, A. and Van Boekel, M.A.J.S. 1999 Dairy Technology:
Principles of Milk Properties & Process: Marcel Dekker, New York.
Frandsen, J.H.and Arbuckle, W.S. 1961. Ice Cream and Related Products. The AVI Publishing Company,
Westport, Connecticut
2. Chemistry and Microbiology of Milk and Milk Products for Engineers 2 + 1
Definition, constituents and composition of milk; factors affecting the composition of milk; physical
properties of milk such as density/specific gravity, pH, electrical conductance, specific heat, thermal
conductivity, surface tension, viscosity refractive index, buffering and redox potential; legal standards of
milk, effect of processing treatments like cooling, heating, concentration and homogenization on milk
constituents; changes taking place in milk constituents during manufacture of dairy products; composition
and standards of milk products; introduction to Microbiology; gross morphology of bacteria, yeast, moulds
and viruses; some important genera of microorganisms; nutrition and cultivation of microorganisms;
42
multiplication and growth of bacteria; effect of some factors on microbial growth-pH, oxygen, water
activity;, temp. irradiation and chemicals; microflora of air, water and soil, indicator organisms; BOD of
effluents; microflora of milk; hygienic milk production; sources of microorganisms in milk; milk quality;
microflora of dairy equipment; cleaning and sanitization of dairy plant; microbial spoilage and preservation
of milk; lactic starter cultures; their use in fermented milk products - Dahi, Yoghurt, Cheese etc.,
microbiology of cream, butter, khoa, paneer, milk powder, milk borne diseases and their control; food
poisoning, microbial fermentation; alcohol; vinegar production;
Practical
Estimation of fat, SNF, specific gravity, acidity and pH; determination of surface tension and viscosity of
milk and refractive index and butyrorefractometer reading of ghee; Callibration of butyrometer; pipette and
lactometer; determination of hardness of water; determination of available chlorine in a sanitizer; staining
of microorganisms; Gram/Spore starting- E. coli, Lact. lactis, Staph. aureus, L. acidophilus, B. cereus, S.
cerevisiae, Aspergillus, Penicillium, growth and enumeration of organisms in broth and agar; tests for
coliforms-IMViC; MPN; PCT; completed test; Eijkmann test rapid platform tests for milk quality
evaluation of detergents and sanitizers by capacity and suspension tests; sampling techniques for
microbiological analysis of dairy products; microbiological instrumentation.
Suggested Readings
Allen H; Vernon & Jane P; Southerland 1994 Milk and milk product technology, try and microbiology
Chapman and Hall.
Walstra, P. and Robertzenis 1984 Dairy Chemistry and Physics John Wiley and Sons
Yadav, J. S., Sunita Grover, and Batish, V.K. 1993 Comprehensive Dairy Microbiology Metropolitan
Publishers, New Delhi.
Lansing, M., Prescott, John P. Harleyand Donald, A. Klein 1993. Microbiology. 2nd Edition Wm. C.
Brown Publishers Dubaque, Lowa Melbourne Australia, Oxford England.
Baianu, I.C.1992. Physical Chemistry of Food Process. AVI Publishing Company, New York.
3. Dairy Business Management 2 + 1
Concept of dairy business management, managerial decision making, functions of management. Planning-
objectives, classification of plans, planning related to finance, production and personnel aspects of the
dairy. Organizing- Fundamentals of organizational design, departmentation, principles and delegation of
authority, responsibility and accountability. StaffPersonnel management, planning, selection, introduction,
orientation and training of unskilled and skilled personnel in dairy organizations. Control- Purpose and
types of control, steps in control, characteristics of effective controls, control techniques and methods,
programme evaluation review technique (PERT), inventory control. Project appraisal and monitoring-
Standards and norms of appraisal, monitoring and its tools, management information systems, net present
value and internal rate of return. Demand analysis- Determinants of demand of dairy products,
responsiveness of demand, estimation of product and factor demand, types and approaches for demand
forecasting. Cost analysis- Application of different cost concepts and functions in managerial decisions.
Pricing-Determinants of price of dairy products, pricing under different objectives and market structures,
product differentiation and product-mix decisions. Profit planning and control- Concept, profit planning
and break even analysis in dairy industry.
Practical
Delineation of milk shed area; Case studies for solving problem situations; Demand forecasting; Estimation
of cost of milk procurement and processing; Break-even analysis and breakeven charts; Use of PERT in
dairy industry; Optimisation of product-mix; Estimation of cost of inventory; Economic lot size and other
quantity standards.
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Suggested Readings
James, A.F., Stoner; R. Edward Freeman and Daniel R. Gilbert Jr., 1996 Management Prentice Hall of
India Pvt. Ltd., New Delhi.
Megginson, L.F., Donald, c., Mosley Paul and H. Pietri, 1983 Management. Harper International Edition,
London.
Arther A. Thompson Jr. and A.J. Strickland, 1995 Strategic Management Concept and Cases. Richard D.
Irwin Inc.
V.L.Mote, Samuel Paul and G.S.Gupta, 1977 Managerial Economics Tata McGraw Hill Publishing
Co.Ltd., New Delhi.
Joel Dean, 1979 Managerial Economics Prentice Hall of India Pvt. Ltd., New Delhi.
Stephen Hill, 1999 Managerial Economics: The analysis of business decisions. Macmillan Education Ltd.,
Hampshire.
4. Econometrics 2 +1
Concept of econometrics, relationship among economic variables; parameters and estimates, use and
importance of econometrics in economic analysis. Linear Model- specification of variables, regression
coefficient; constant term, least-squares and its assumptions; estimation of parameters of the model;
properties of least-squares estimates; Gauss-Markov Theorem; simple correlation; fixed and stochastic
regressors, method of estimation, error variance, standard error of regression coefficient. Coefficient of
determination. Test of significance and prediction. Nonlinear models. Extension of two variable linear
regression model- estimation of parameters, partial correlation coefficients, coefficient of multiple
determination, test of significance of least squares estimates. Regression analysis and analysis of variance.
Testing the overall significance of regression, improvement of fit from additional regressors. Chow test.
Consequences of left out variables. Heteroscedasticity- sources, tests, consequences and solutions. Auto-
correlationsources, consequences, Durbin-Watson test; method of estimation and prediction. Solutions for
the case of auto-correlation. Multicollinearity- tests, consequences and possible remedies. Dummy
variables and its applications. Introduction to distributed Lag Models-Koyck's Geometric lag scheme.
Practical
Estimation of parameters of single equation two variable linear model, test of significance of estimate of
parameters, coefficient of determination and economic interpretation, estimation of non-linear two variable
regression model. Estimation of parameters of three variable linear model; coefficient of multiple
determination, regression analysis and analysis of variance.
Suggested Readings
Johnston, J. 1984. Econometric Methods. McGraw Hill.
Gujarati, D.N. 1995. Basic Econometric. McGraw Hill
Kmenta, J. 1971. Elements of Econometrics McMillan, New York.
Theil, H. 1978. Introduction to Econometric. Prentice Hall of India Pvt.Ltd., New Delhi.
Maddala, G.S.1977 Econometrics, International Student Edition. McGraw Hill, Bogalusa Ltd., Tokyo.
5. Statistics for Industrial Applications 2 + 1
Statistical methods in industrial applications; analysis of variance; transformations; partial and multiple
correlation and regression; Ranking techniques; introduction to discriminant analysis; statistical basis for
drawing scientific inferences from experimental data; principles of experimental design-industrial
experimentation; basic designs-CRD; RED & LSD; missing plot technique; factorial experiments-main
44
effects and interactions; 2n series and mixed factorial experiments; experimental designs in sensory
evaluation; introduction to statistical quality control; control charts for variables; mean and range charts;
statistical basis; rational sub-group; control charts for attributes 'np'; 'p' and 'c' charts; fundamental concepts
of acceptance sampling plans; single; double and sequential sampling plans; use of sampling inspection
tables for selection of single and double sampling plans; introduction to sampling techniques and their
application to consumer preference studies.
Practical
Analysis of variance-one way and two way classification; partial and multiple correlation and regression;
rank correlation and coefficient of concordance; analysis of industrial experiments Use of CRD; RBD and
LSD; missing plot technique; factorial experiments - 22 and 23; mixed factorial experiments; control charts
for variables; control charts for attributes; single sampling plan- OC and AOQ curves; sequential sampling
plan; use of sampling inspection tables; and different methods of selecting samples;
Suggested Readings
Snedecor; G. W. and Cochran, W.G. 1968 Statistical Methods Oxford and IBH Publishing Co., New Delhi
Cochran, W. G. 1972 Sampling Techniques. Wiley Eastern Pvt. Ltd., New York
Cochran, W. G. and G.M. Cox 1962 Experimental Designs Asia Publishing House, Bombay Grant and
Leavenworth 1972 Statistical Quality Control, McGraw Hill, New Delhi
Indian Standards Institute 1986 New Delhi Handbook on Statistical Quality Control.
6. Research Documentation 1 + 1
Library as information centre; information technology; CD ROM data search; abstracts and abstracting
techniques; the concept of documentation; international network/Indian scene; information retrieval
systems; MIS; science citation; web internet search; presentation of results of research; different kinds of
scientific writings: writing of scientific reports, thesis, research papers etc; oral and poster presentation of
a paper in conferences/symposia; standard abbreviations used in proof reading; printing technology and
corrections of manuscripts.
Practical
Different schemes of classification & various cataloguing codes for arranging documents; use of
catalogues & classification; types of libraries & standard sources of information; familiarity in use of
standard sources of information; exercise in abstracting of research articles; demonstration of
photography; photocopying; printing; preparation and handling of audio-visual aids; preparation of
multimedia; exercise in proof corrections; research paper writing & interpretation; CD-ROM search; and
Internet search.
Suggested Readings
Balaguru, T. 1996. Management Information Systems for Agricultural Research, NAARM, Hyderabad.
Chhotey Lal 1998. Agricultural Libraries & Information Systems: A Handbook for Users. R.K. Techno
Science Agency, New Delhi.
Guha, B. 1987. Documentation & Information. World Press Pvt. Ltd., Kolkatta
Kaula, P.N. etc. 1996. International & Comparative Librarianship and Information Systems. B.R.
Publishing Corpn., Delhi.
Venkatasubramanian, V. 1999. Introduction to Research Methodology in Agricultural & Biologial
Sciences. New Century Book House, Chennai.
Sharma, P.S.K. 1987 Library & Society. Ess Ess Publication, New Delhi. CBF Style Manual Published by
Council of Biology, Maryland.
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