nd midwest postdoctoral symposium iowa state … midwest postdoctoral symposium iowa state...

2nd Midwest Postdoctoral Symposium

Iowa State University

May 15th 2015 – 8:00 AM to 6:30 PMAmes - Iowa

ORGANIZED BY IOWA STATE UNIVERSITY POSTDOCTORAL ASSOCIATION

HTTP://WWW.PDA.IASTATE.EDU/NEWS/EVENT/MPS2015All events will take place at Iowa State University Molecular Biology Building. Iowa State

University | Ames, IA 50011-3260The symposium is sponsored by Iowa State Graduate College

All headers images courtesy of University Archives, ISU Library.

Contents

Contents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . III

Program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1

Morning Sessions 1Afternoon Sessions 2Poster session 2Oral sessions 3

Keynote and Panelists Bios . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

Keynote speakers 5Panel 1: From postdoc to academia 7Panel 2: From postdoc to industry 9Panel 3: From postdoc to entrepreneurship 11

Abstracts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

Poster Presentations 13Medicine - Other . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13Engineering . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19Agriculture and Life Sciences . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22Chemistry and Biochemistry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

Oral Presentations 28Teaching and Human Behavior . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28Agriculture and Life Sciences . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31Chemistry and Mathematics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34Materials Science and Engineering . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37Biomedicine, Veterinary Medicine and Molecular Biology . . . . . . . . . . . . . . . . 41

IV

List of presenters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

List of keywords . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

Symposium Organizers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

Directions and Lunch Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51

Campus map 51Molecular biology building map 52Lunch Buffet Menu 53

Program

Morning Sessions8:00 AM - 10:00 AM

Registration, breakfast, networking – Atrium

10:00 AM - 11:00 AMOpening. Keynote by Dr. Howard E. Gendelman (University of Nebraska) – Auditorium(Room 1414)

11:00 AM - 12:00 AMOral presentations

Teaching and Human Behavior – Room 1420Agriculture and Life Sciences – Room 1414Chemistry and Mathematics – Room 1424

12:00 AM - 1:00 PMLunch* – Atrium and Room 1102*Only for registered participants - vegetarian and gluten free options available

2 Afternoon Sessions Program

Afternoon Sessions1:00 PM - 2:00 PM

Keynote by Dr. Adam Schwartz (The Ames Laboratory, US DoE) – Auditorium (Room1414)

2:00 PM - 3:00 PMPanel Sessions:

PANEL 1: From postdoc to academia – Room 1420PANEL 2: From postdoc to industry – Room 1414PANEL 3: From postdoc to entrepreneurship – Room 1424

3:00 PM - 4:30 PMPosters + coffee break – Atrium

4:30 PM - 6:00 PMOral presentations

Materials Science and Engineering – Room 1420Biomedicine, Veterinary Medicine and Molecular Biology – Room 1414

6:00 PM - 6:30 PMClosing remarks, awards, prizes – Auditorium (Room 1414)

Poster sessionPoster/networking session will be held in the Atrium of the Molecular Biology Building (buildingmap on page 52).Posters should be set up before 3:00 PM and removed by the end of the day.

Author Title Poster IDMEDICINE AND BIOMEDICAL SCIENCES

Andrea Gutman ASIC1a in the nucleus accumbens regulates cocaine-associated behaviors

P1

Svitlana Zbarska A peripheral nerve regeneration strategy using a com-bination of bone marrow-derived stem cells transd-ifferentiated into Schwann-like cells and micropat-terned biodegradable polymer conduits

P2

Jill A. Madden Evidence for modulation of FoxM1 by p21 in ovariancancer

P3

Ryan Kolb The NLRC4 inflammasome promotes breast cancerprogression in diet-induced obese mice

P4

Andrea Gutman Advocacy for biomedical research during early careerstages

P5

Emily K. Blue Epigenetic regulation in neonatal ECFCs followingintrauterine exposure to gestational diabetes

P6

ENGINEERINGNikolai A. Zarkevich Pressure-induced magneto-structural transition in

ironP7

Md Azahar Ali Graphene Oxide Decorated PEDOT Nanofibers forSensing of Nitrate Ions

P8

Continued on next page

3 Oral sessions Program

Continued from previous pageAuthor Title Poster IDBhavna Sharma GIS-based suitability model for siting bioethanol

plants in the U.S.P9

AGRICULTURE AND LIFE SCIENCESJuliette Doumayrou Recovering grass-infecting viruses in historical

worldwide herbarium specimens to understand theirevolution through time

P10

Eugenia E. Montiel Jimenez Structure and evolution of the youngest sex chromo-some system in turtles

P11

Jasreen K. Sekhon Effects of co-products of enzyme-assisted aqueousextraction of soybeans on ethanol production in cornfermentation

P12

CHEMISTRY AND BIOCHEMISTRYDavid Lynch Genotypic Diversity of Streptococcus sobrinus in

American Indian ChildrenP13

Naftali N. Opembe Exploiting Soft Template Synthesized Ordered Meso-porous Carbons for Catalysis

P14

Brajesh K. Singh Rapid spread of measles virus in primary humanairway epithelia

P15

Oral sessions

Author Title Pres. IDTEACHING AND HUMAN BEHAVIOR • ROOM 1420 – 11:00 AM

Bhavna Sharma Teaching as Research: Understanding students’ per-ceptions and knowledge of an interdisciplinary engi-neering course

O1

Carol L. Chaffee Teaching biodiversity positively influences both thecognitive and affective domains

O2

Cara Hamann Naturalistic study of adult and child bicycling behav-iors and risk exposure

O3

AGRICULTURE AND LIFE SCIENCES • ROOM 1414 – 11:00 AMSung Ki Cho Polypyrimidine tract-binding proteins regulate RNA

metabolism of a mobile RNA for potato tuberizationO4

Amit Khanchi Estimating dry matter and composition loss due torainfall during field drying of switchgrass and cornstover

O5

Jennifer Jandt Effects of maternal behavior and nutrition on caste-biased gene expression during larval development inPolistes wasps

O6

CHEMISTRY AND MATHEMATICS • ROOM 1424 – 11:00 AMXiang-Wei Du Rhodium-Catalyzed Regioselective Hydroacylation

of Alkynes and Its Application in the Total Synthesisof Isoflavones: Daidzein and Formononetin

O7

Continued on next page

4 Oral sessions Program

Continued from previous pageAuthor Title Pres. IDArmando D. Estillore Probing the chemistry and properties of sea spray

aerosol using single particle microscopy and spec-troscopy techniques

O8

Terrance Pendleton Elastic collisions among peakon solutions for theCamassa-Holm equation

O9

MATERIALS SCIENCE AND ENGINEERING • ROOM 1420 – 4:30 PMSimge Cinar Particles for joining and manufacturing of metals at

room temperatureO10

Prashant Singh Concentration waves in high-entropy alloys – a novelalloy design approach

O11

Ashokkumar M. Sharma Cleaning of biomass-generated syngas for productionof biofuels and biochemicals

O12

Ali Jahanbani Ardakani Identification of umbrella constraints in DC-basedsecurity-constrained optimal power flow

O13

BIOMEDICINE, VETERINARY MEDICINE AND MOLECULAR BIOLOGY • ROOM – 1414 4:30 PMMadhusudana Rao Chikka Activation of the innate immune response upon mito-

chondrial dysfunction protects against neurodegener-ation in C. elegans

O14

Sina Safayi Use of computerized 3-D motion analysis as a biome-chanical assessment tool in biomedical studies in-vivo

O15

Paisley Canning Determination of the concentration of tylvalosin inthe synovial fluid of swine after administration at adose of 5mg/kg by oral gavage

O16

Kais Zakharia The effect of CX-4945, a CK2 inhibitor, on cholan-giocarcinoma (CCA): in vitro data

O17

Keynote and Panelists Bios

Keynote speakers

DR. HOWARD E.GENDELMAN is theMargaret R. LarsonProfessor of Inter-nal Medicine andInfectious Diseases,Chairman of the De-partment of Phar-macology and Ex-perimental Neuro-science, and Direc-tor of the Center for

Neurodegenerative Disorders at the Universityof Nebraska Medical Center. Dr. Gendelmanis credited in unraveling how functional al-terations in brain immunity induce metabolicchanges and ultimately lead to neural cell dam-age for a broad range of infectious, metabolicand neurodegenerative disorders. These dis-coveries have had broad implications in de-velopmental therapeutics aimed at preventing,slowing or reversing neural maladies. He isalso credited for the demonstration that AIDSdementia is a reversible metabolic encephalopa-thy; a finding realized at the University ofNebraska Medical Center. His work has ledto novel immunotherapy and nanomedicinestrategies for Parkinson’s and viral diseasescurrently being tested in early clinical trials asa result of intense translational investigations.

Dr. Gendelman obtained a Bachelor’s degreein Natural Sciences and Russian Studies withhonors from Muhlenberg College and hisM.D. from the Pennsylvania State University-Hershey Medical Center where he was the 1999Distinguished Alumnus. He completed a resi-dency in Internal medicine at Montefiore Hos-pital, Albert Einstein College of Medicine andwas a Clinical and Research Fellow in Neu-rology and Infectious Diseases at the JohnsHopkins University Medical Center. He oc-cupied senior faculty and research positions atthe Johns Hopkins Medical Institutions, the Na-tional Institute of Allergy and Infectious Dis-eases, the Uniformed Services University ofthe Health Sciences Center, the Walter ReedArmy Institute of Research, and the Henry Jack-son Foundation for the Advancement in Mili-tary Medicine before joining the University ofNebraska Medical Center faculty in March of1993.

He retired from the US Army with the rankof Lieutenant Colonel. Dr. Gendelman hasauthored over 400 peer-reviewed publications,edited nine books and monographs, holds eightpatents, is the Editor-In-Chief and Founderof the Journal of Neuroimmune Pharmacol-ogy along with service on numerous editorialboards, national and international scientific re-view and federal and state committees. He has

6 Keynote speakers Keynote and Panelists Bios

been an invited lecturer to more than 200 scien-tific seminars and symposia and the recipientof numerous local, national and internationalhonors. These, include, but are not limitedto, the Henry L. Moses Award in Basic Sci-ence; the Carter-Wallace Fellow for Distinc-tion in AIDS Research, the David T. PurtiloDistinguished Chair of Pathology and Micro-biology, the UNMC Scientist Laureate; NUOutstanding Research and Creativity and theJoseph Wybran Distinguished Scientist Awards.Dr. Gendelman was named a J. William Ful-bright Research Scholar at the Weizmann Insti-tute of Science in Israel.In 2001, he received the prestigious Jacob JavitsNeuroscience Research Award and the CareerResearch Award in Medicine from UNMC. Heis included amongst a selective scientific grouplisted on highly cited.com as one of the topcited scientists in his field. Dr. Gendelman hastrained more than forty scientists (students andpostdoctoral fellows) who have themselves de-veloped independent successful careers. Underhis leadership, the department now holds scoresof independent R01s or equivalent grants, fourprogram project grants, and shares two pro-gram developmental awards. His leadership iscredited with the growth of the Department ofPharmacology and Experimental Neuroscienceat the University of Nebraska Medical Centerto be amongst the top-like ranked and federallyfunded departments (top ten) nationwide; a par-ticularly noted feat as its position was 77 whenhe assumed its leadership.

DR. ADAM

SCHWARTZ re-ceived his B.S in1985 and M.S in1989 from the de-partment of metal-lurgical engineeringat the University ofPittsburg, Pennsyl-vania. He receivedhis Ph.D in mate-

rials science and engineering in 1991 fromthe University of Pittsburg. Afterwards, hemoved on to Lawrence Livermore NationalLaboratory in California where he started as apostdoctoral research associate and eventuallybecame the division leader of the condensedmatter and materials division in 2010. In 2013,he joined the leadership team of the criticalmaterials institute of the Ames Laboratory inIowa. He became the director of the AmesLaboratory and a tenured professor of Mate-rials Science and Engineering at Iowa StateUniversity in 2014. Professor Schwartz is anaccomplished researcher whose work has fo-cused on plutonium aging and alloys, advancedcharacterization, and the dynamic properties ofmaterials. Professor Schwartz received manyprestigious awards including the Neill GriffithsMemorial Award in 1998, the DOE andNNSADefense Programs Award of Excellence in2007 as well as the LLNL Science, Technology,Engineering, and Operations Award in 2008.

7 Panel 1: From postdoc to academia Keynote and Panelists Bios

Panel 1: From postdoc to academia

DR. MELISSA

BATES is Assis-tant Professor ofHealth and HumanPhysiology at theUniversity of Iowa.She completed herPhD in Physiologyat The Pennsylva-nia State Univer-sity and a postdoc-toral fellowship inPediatric CriticalCare Medicine in

the Rankin Laboratory of Pulmonary Medicineat the University of Wisconsin. Her lab’s mis-sion is to understand how events that happenimmediately after birth impact long-term heartand lung development, especially in terms ofthe ability to respond to subsequent stressesincluding altitude, sleep disordered breathingand anesthesia.

DR. BRIAN GELDER

grew up on, and stillhelps with, a corn,soybean, and hogfarm about 30 milesnorth of Ames nearJewell, Iowa. Heattended Iowa Cen-tral Community Col-lege and Iowa StateUniversity, receiv-ing a double B.S. inAgronomy and Ag

Engineering in 2000. He then received a MSfrom Colorado State in Bioresource Engineer-ing in 2002 and a PhD from Iowa State inSoil Science and Ag Engineering in 2007 fordeveloping methods to determine crop rotation,residue cover, and surface roughness via re-mote sensing. He then began a postdoc withRob Anex (now of UW-Madison) until 2010where he worked on estimating impacts of re-gional biofuel production scenarios before hetransitioned into an Associate Scientist posi-tion. He now researches methods to remotely

sense residue cover, enforce digital elevationmodels automatically, and estimate erosion onhillslopes across multiple states.

DR. SAL MEYERS

has taught at Simp-son College for 20years and is cur-rently Professor ofPsychology and Di-rector of Faculty De-velopment. She hasserved on numer-ous faculty searchcommittees and cananswer questionsabout what small

liberal arts colleges are looking for when theyhire. Sal earned her Ph.D. in social psychologyat the University of Minnesota.

DR. GRETCHEN

A. MOSHER is anassistant professorin Agricultural andBiosystems Engi-neering. She holds aresearch and teach-ing appointment.Research interestsinclude food sys-tem safety, interac-tions between qual-ity managementand other opera-

tional goals, and factors influencing how stu-dents learn and utilize complex information.Dr. Mosher teaches Total Quality Managementand the Technology Capstone and serves herdepartment, college, and profession in a varietyof capacities.

8 Panel 1: From postdoc to academia Keynote and Panelists Bios

DR. BRET ULERY

is an assistant pro-fessor in the Chemi-cal Engineering De-partment at the Uni-versity of Missourihaving started in Au-gust 2014. Af-ter earning a B.S.E.in Chemical Engi-neering and B.S. inBiochemistry fromthe University ofIowa in 2006, he

conducted graduate research with BalajiNarasimhan at Iowa State University and re-ceived his Ph.D. in Chemical Engineering witha Graduate Minor in Immunobiology in 2010.Following completion of his doctoral studies,he spent two years as a Postdoctoral Fellow inCato Laurencin’s research group in the Institutefor Regenerative Engineering at the Universityof Connecticut Health Center followed by ayear and half as a Postdoctoral Scholar underthe advisement of Matt Tirell in the Institutefor Molecular Engineering at the University ofChicago.

9 Panel 2: From postdoc to industry Keynote and Panelists Bios

Panel 2: From postdoc to industry

DR. MICHAEL MC-CARVILLE joinedBayer CropSciencein 2014 as a Seed-Growth TechnicalService. This roleprovides Dr. Mc-Carville to workwith seed compa-nies, agricultural

distribution companies, and cooperatives toresearch and design solutions for farmers. Be-fore joining Bayer, Dr. McCarville was apostdoctoral research associate and graduate re-search assistant in the Entomology Departmentat Iowa State University. His research focusedon the ecology and management of insects andnematodes in agricultural crops.

DR. DAVE AUSTIN

is the Director ofthe Crop GeneticsInformatics groupat DuPont Pioneer,located in Johnston,Iowa. His responsi-bilities include lead-ing software devel-opment, softwaresupport, and datamanagement for Pi-oneer product de-velopment scientists

located in 25 countries worldwide.Dave began his career at Pioneer in 1997 in theResearch Information Management departmentserving as a support manager for corn productdevelopment and breeding technologies. Heplayed a key role in the development of sys-tems to link field and laboratory data to developsuperior products for Pioneer customers.Dave is originally from Wisconsin, where hegrew up on a dairy farm near the town of Dar-lington. He received his B.S. degree in Agron-omy from the University of Wisconsin. Heholds Master of Science and Doctor of Philos-ophy degrees in Plant Breeding and Geneticsfrom Iowa State University.

Dave and his wife, Mindy, reside in Ankenywith their three teenage children. Their daugh-ter is wrapping up her first year of studies atIowa State University. Dave currently serves onthe Board of Directors at Living History Farmsin Urbandale, Iowa. He enjoys the opportunityto help connect the current generation to ourrich agricultural history. He also has a passionfor photography and volunteers his time tak-ing photos for Living History Farms, LutheranChurch of Hope, and Ankeny High School ath-letic events.

DR. BRENDA

CARRILLO-CONDE is a SeniorResearch Scientistin Bioprocess R&Dat Pfizer Inc. Shereceived her Bach-elor of Science inChemical Engineer-ing with a minor inEnvironmental Sys-tems in 2006 fromthe Monterrey In-

stitute of Technology and Higher Education(ITESM) in Mexico. In 2011, she got herPh.D. in Chemical and Biological Engineeringat Iowa State University. Previous to joiningPfizer, Dr. Carrillo-Conde spent two years as apostdoctoral fellow affiliated to both the Chem-ical and Biomedical Engineering Departmentsat The University of Texas at Austin. Bothher Ph.D. and postdoctoral research focusedon the design, engineering, and evaluation ofbiomaterial-based platforms for targeted drugand vaccine delivery. Her research in this arealed to more than 20 peer-reviewed publicationsin journals such as Scientific Reports, ScienceTranslational Medicine, Molecular Pharmaceu-tics, and Acta Biomaterialia. As a Senior Sci-entist in the Conjugation and Polytides ProcessDevelopment group at Pfizer, she is responsiblefor the design of scalable, robust, efficient, andinnovative chemical and biochemical conju-gation processes to support the vaccine andantibody-drug conjugate (ADC) portfolio. She

10 Panel 2: From postdoc to industry Keynote and Panelists Bios

leads technology transfer of conjugate vaccinesprocesses to pilot and commercial facilitiesfor the production of clinical and commercialmaterials.

DR. KATRYN

ALLEN. I graduatedfrom Bethany Col-lege in Lindsborg,Kansas in 2005with degrees in bi-ology and chem-istry. I then startedgraduate school atthe University ofKansas Medical cen-

ter in Kansas City, where I worked under thedirection of Dr. Bryan Copple. My researchwas focused on mechanism of liver injury dur-ing cholestasis. I received my Ph.D. in 2011and started my Post-doctoral fellowship atMichigan State University, in the laboratoryof Dr. Jack Harkema. While at MSU I was in-volved with the postdoc association, and was aco-chair from 2013 to 2014. I started in my cur-rent role as a Study Director with XenometicsLLC in February of 2014.

11 Panel 3: From postdoc to entrepreneurship Keynote and Panelists Bios

Panel 3: From postdoc to entrepreneurship

DR. ANKIT AGAR-WAL is CEO andfounder of ImbedBiosciences Inc, adevelopment stagecompany commer-cializing advancedmaterials for woundhealing and sur-gical applications.Imbed’s technologyplatform on poly-

meric nanofilms was invented by Agarwalduring his post-doctoral research at the Univer-sity of Wisconsin-Madison. Starting in 2012,Imbed has raised $2.5M in private and federalfunding. Company is working on receivingFDA regulatory clearance to market their firstadvanced wound-dressing product containingsilver nanoparticles. Agarwal received oneyear training in entrepreneurship as a fellowof the Kauffman Foundation. He did his BSfrom Indian Institute of Technology, Delhiand Ph.D. in Chemical Engineering from IowaState University.

DR. LISA LOREN-ZEN is the ExecutiveDirector of the ISUResearch Founda-tion and Director ofthe Office of Intel-lectual Property andTechnology Trans-fer OIPTT), focusedon technology trans-fer and economic de-velopment. The ISUResearch Founda-tion owns and man-

ages intellectual property on behalf of IowaState University. The Office of Intellectual

Property and Technology Transfer markets andlicenses intellectual property and negotiatescontracts related to sponsored research withindustry and manages grant programs relatedto economic development.Before joining ISU in March 1999, Lisa workedfor five years as a computational biologist inthe bioinformatics group at Pioneer Hi-BredInternational, Inc.Lisa graduated from Iowa State University witha B.S. degree in 1989 and Ph.D. in 1994, bothin Genetics.

DR. SHIVANI

GARG is a bio-chemist andan entrepreneur,who founded herbiotech start-up,OmegaChea Biore-newables LLC,while pursuing herdoctorate degree in

Biochemistry at Iowa State University. Orig-inally from India, Shivani has a backgroundin biotechnology, enzyme and microbial en-gineering, and is passionate about developingsustainable technologies for producing bio-renewables. Her start-up, which is a spin-offfrom NSF Engineering Research Center forBiorenewable Chemicals (CBiRC), focuses onproduction of bio-based chemicals using engi-neered bacterial hosts. These chemicals canserve as sustainable alternatives to petroleumbased feedstocks in the current chemical in-dustry, specifically in detergents and lubricantsmarkets. Shivani was awarded NSF InnovationCorps award in 2012 for business model devel-opment for the start-up, and later OmegaCheawas awarded NSF Small Business TechnologyTransfer (STTR) Phase I award in 2013 alongwith several state funding awards.

Abstracts

Poster PresentationsMedicine - OtherASIC1a in the nucleus accumbens regulates cocaine-associated behaviorsAndrea Gutman1

1University of IowaAbstract

Acid-sensing ion channels (ASICs) are membrane-bound cation channels that are activated by acidosisof the extracellular fluid. ASIC1a, a subunit of the ASIC1 channel, is expressed at high levelsin the nucleus accumbens (NA), a region that regulates addiction-related behaviors. This studyinvestigated whether ASIC1a in NA regulates two addiction-related behaviors: cocaine conditionedplace preference (CPP) and cocaine self-administration. Asic1a-/- mice were used to examine thecontribution of ASIC1a to cocaine-associated learning memory. Mice then underwent CPP, in whichthey were administered cocaine (10 mg/kg) and confined to one side of a two-sided chamber (thecocaine-associated context). During testing, Asic1a-/- mice showed greater cocaine CPP relativeto controls. To determine whether ASIC1a in the NA was responsible for these effects, ASIC1aexpression was restored in Asic1a-/- mice by injecting AAV-Asic1a into the NA. Rescue of ASIC1ain the NA reversed the increased CPP found in the knockout mice. To confirm the involvementof the NA, ASIC1a expression was disrupted in the NAc using Asic1a loxP/loxP mice. Selectivedisruption of ASIC1a in the NA significantly increased cocaine CPP. To examine the role of ASIC1ain cocaine self-administration, ASIC1a was bilaterally overexpressed in the NA core of rats. Ratswere trained to self-administer cocaine in daily two-hour sessions during which lever presses resultedin an intrajugular cocaine infusion. Rats underwent one day of self-administration with each of fivecocaine doses (300, 90, 30, 9, 3 µg/infusion) to create a dose-response curve. Overexpression ofASIC1a in the NA of rats reduced cocaine self-administration relative to AAV2/1-CMV-eGFP controlsand caused a rightward shift in the dose-response curve. Together, these results suggest that ASIC1ain the NA is involved in the rewarding properties of cocaine and, moreover, point to a novel potentialtherapeutic target for drug addiction.

Poster IDP1.

KeywordsAddiction, ASICs, Cocaine, Nucleus accumbens.

14 Abstracts - Poster Presentations

A peripheral nerve regeneration strategy using a combination of bone marrow-derivedstem cells transdifferentiated into Schwann-like cells and micropatterned biodegrad-able polymer conduitsSvitlana Zbarska1,2, Sharma A.D.1,3, Mallapragada S.K.1,3, Sakaguchi D.S.1,2

1Neuroscience Program – Iowa State University, 2Department of Genetics, Development and Cell Biology –Iowa State University, 3Department of Chemical and Biological Engineering – Iowa State University

AbstractPeripheral nerve injuries (PNI) can lead to serious neurological deficits resulting in sensory/motordysfunctions, including paralysis. Autologous nerve grafts including Schwann cells (SCs) areconsidered the “gold standard” for treatment of severe PNI. SCs are peripheral glia forming themyelin sheath around axons of motor and sensory neurons. Previously we have demonstrated that SCsimplanted inside of micropatterned nerve regeneration conduits (NRC) enhanced peripheral nerveregeneration. However, SC harvesting requires an additional surgery and sacrifice of a donor nervethat results in donor site morbidity. Due to these limitations, alternative cell sources and strategies arebeing investigated.Recently we have shown that rat bone marrow-derived mesenchymal stem cells (MSCs) can betransdifferentiated into SC-like phenotypes on micropatterned biodegradable poly-lactic acid (PLA)substrates. These results revealed that substrate topography strongly influenced the morphology andgrowth of the MSCs. Cells aligned in the direction of the grooves when grown on micropatternedPLA substrates, and micropatterning did not impact the level of transdifferentiation.In our ongoing project we are combining micropatterned topographical cues in a NRC and biologicalcues in the form of transdifferentiated rat bone marrow-derived cells. Three groups of Brown Norwayrats were implanted with micropatterned PLA NRCs to bridge a 10 mm sciatic nerve transection gap.Group 1 was implanted with control, empty conduits; Group 2 was implanted with conduits pre-seededwith tMSCs (Schwann-like cells) or undifferentiated MSCs (uMSCs). Both populations of MSCsexpress green fluorescent protein (GFP). After implantation animals are assessed for possible recoveryof motor and sensory function. In the future, in addition to pre-seeded tMSC-NRCs, we plan toincorporate neurotrophic factor encapsulated nanoparticles to enhance peripheral nerve regeneration.

Poster IDP2.

KeywordsBone, Peripheral nerve regeneration, Schwann cells.


Evidence for modulation of FoxM1 by p21 in ovarian cancerJill A. Madden1, J. Chien1University of Kansas Medical Center

AbstractThe oncogenic transcription factor forkhead box M1 (FoxM1) is overexpressed in many cancers,including 84% of ovarian cancer. It plays a role in DNA repair, mitotic checkpoint, cell proliferation,and cancer drug resistance, but the regulation of FoxM1 has yet to be elucidated. Evidence suggestsFoxM1 up-regulation is a result of TP53 mutations, however, no TP53 response element has beenfound in the FoxM1 promoter. We hypothesize that cyclin-dependent kinase inhibitor 1a, p21, isinvolved in the regulation of FoxM1 by p53 in ovarian cancer. To test this hypothesis, we knockeddown p21 expression with siRNA in two wild-type p53 cancer cell lines, NCI-H23 and A2780. Knockdown of p21 resulted in a mirrored increase in FoxM1 protein level in both cell lines as detectedby Western blot. The addition of nutlin-3 (10 µM), a p53 stabilizer, to these cell lines restoredsome p21 expression 24 hour post-treatment, which led to a subsequent decrease in FoxM1 levelscompared to control. These results suggest p21 is a negative regulator of FoxM1 in cancer cell lines.To further investigate, the same cancer cell lines were treated with FoxM1 inhibitor thiostrepton (10µM) and protein levels were measured over a time course of 48 hrs. FoxM1 protein levels werereduced following 8 hours, a time point where p21 protein levels peaked. This finding was furthersupport by qRT-PCR analysis of A2780 ovarian cancer cell mRNA collected 0, 6, 8, 9, 10, 11, and 12hrs following thiostrepton (10 µM) treatment. In this experiment, p21 mRNA levels peaked 8 hrspost-treatment, with FoxM1 mRNA levels reduced at 6 hrs post-treatment, relative to control. Theseresults suggest that thiostrepton may also induce p21, potentiating FoxM1 inhibition. Thiostreptonhas previously been shown to induce cancer cell death and reduce tumor burden, therefore furthersupporting FoxM1 as a potential drug target as well as encouraging the need to understand regulatorsin this pathway.

Poster IDP3.

KeywordsCancer therapy, FoxM1, Gene regulation, Ovarian cancer.


The NLRC4 inflammasome promotes breast cancer progression in diet-induced obesemiceRyan Kolb1, Nicholas Borcherding, Yinghong Liu, Fang Yuan, Qing Xie, Fayyaz Sutterwala,Weizhou Zhang1Department of Pathology – University of Iowa

AbstractObesity is not only a known risk factor for the development of ER+ breast cancer in postmenopausalwoman, but is also associated with a poor clinical outcome across all subtypes of breast cancer.Obesity is also one of the only known risk factors for the development of invasive ductal carcinoma(IDC) versus ductal carcinoma in-situ (DCIS; non-invasive). A number of hypotheses have beenproposed to explain the association between obesity and breast cancer progression, however, themechanism linking obesity and breast cancer progression remains unknown. We hypothesize thatobesity-induced inflammasome activation in mammary adipose tissue results in active IL-1 which inturn promotes breast cancer progression. Using a syngeneic orthotopic transplant model of breastcancer in C57Bl/6 mice, we have shown that diet-induced obese (DIO) mice (mice fed a high fatdiet) have increased tumor growth, macrophage infiltration and caspase-1 activation compared totumors from control mice (mice fed normal chow). Additional studies have shown that treating DIOmice with an IL1R1 (the receptor for IL-1) blocking antibody reduces tumor growth to that observedin control mice. Furthermore, NLRC4 deficient DIO mice had reduced tumor growth (comparedto wild-type DIO) mice, indicating that NLRC4 inflammasome activation promotes breast cancergrowth in obese mice. Finally, tumors from DIO mice had increased vascularization (as evidencedby an increase in CD31+ cells) compared to control mice, which was decreased in NLRC4 deficientmice or mice treated with the anti-IL1R1 antibody. This data indicates that obesity induced NLRC4inflammasome activation in infiltrating macrophages in the mammary gland promotes breast cancerprogression possibly through increased angiogenesis in the tumor microenvironment. Thus, targetinginflammasome activation or the IL-1/IL1R1 axis represents a rational target for the treatment of breastcancer in obese patients.

Poster IDP4.

KeywordsAngiogenesis, Cancer, Inflammation, NLRC4, Obesity.


Advocacy for biomedical research during early career stagesAndrea Gutman1

1University of Iowa

AbstractTwo years ago, federal spending cuts eliminated $1.5 billion from the National Institutes of Healthbudget and $356 million from the National Science Foundation. While there has been some reprievefrom these cuts, “sequestration” could return later in 2015. Even without sequestration, federalscientific funding remains anemic. Federal funding has been roughly stagnant since 2003 and hasdecreased since 2013. With inflation is taken into account, this has led to a significant loss ofpurchasing power among biomedical scientists who rely on this funding.What can be done about this? As scientists, we have a responsibility to advocate for ourselvesand speak up for research, even during the early stages of our careers. Four ways in which to dothis include: 1. Staying informed about current policy and advocacy issues, 2. Communicatingwith congress, 3. Spreading the word to fellow scientists, and 4. Public outreach. Perhaps themost important of these is communicating directly with policy makers to let them know that theirconstituents support funding for biomedical research. Lab tours can also be an effective way ofshowing legislators how federal funds are spent and highlighting important advances in your field.This poster focuses on the current state of biomedical research funding and how individuals in sciencecareers can advocate for continued investment in biomedical research.

Poster IDP5.

KeywordsAdvocacy, Funding, National Institutes of Health (NIH), National Science Foundation (NSF), Policy.


Epigenetic regulation in neonatal ECFCs following intrauterine exposure to gesta-tional diabetesEmily K. Blue1, BreAnn M. Sheehan, Zia V. Nuss, Frances A. Boyle, Caleb M. Hocutt, CassandraR. Gohn, Kaela M. Varberg, Jeanette N. McClintick, and Laura S. Haneline1School of Medicine – Indiana University

AbstractGestational diabetes (GDM) complicates up to 10% of pregnancies. The children of diabetic mothershave an increased risk of obesity, diabetes, and hypertension, starting in childhood. While the causesof this increased risk are unknown, previous studies in our lab have identified functional deficits inhuman endothelial colony forming cells (ECFCs) isolated from the cord blood of GDM pregnancies.The objective of this study was to identify mRNA expression and DNA methylation alterations inECFCs that may underlie ECFC dysfunction following intrauterine exposure to GDM. ECFCs wereobtained from control and GDM pregnancies. DNA, RNA, and protein samples were isolated inparallel. RNA microarray analysis was used to identify gene expression alterations in GDM ECFCscompared to control ECFCs. Genome-wide DNA methylation status at >450,000 CpG sites wasassessed using a DNA methylation array. RNA expression results were validated using qRT-PCRand western blotting. Bisulfite sequencing of genomic DNA from the ECFCs was performed toidentify additional sites with altered methylation for regions not included in the DNA methylationarray. Of the 28,000 genetic loci tested, 596 mRNAs were altered between control and GDM ECFCs.PLAC8 was identified as being increased 5-fold by microarray analysis, a result which was confirmedin two cohorts by qRT-PCR and western blotting. Analysis of the methylation array and bisulfitesequencing results revealed 3 regions surrounding the transcriptional start site of PLAC8 gene whoseCpG methylation negatively correlate with RNA expression in samples from control and GDM ECFCs.The discovery of 20 CpG sites whose methylation correlates with PLAC8 mRNA expression in ECFCsis consistent with the hypothesis that intrauterine exposure to GDM results in epigenetic changes.Analysis of methylation at these sites or others could be used as a biomarker for children of motherswith GDM who may be at risk for disease later in life.

Poster IDP6.

KeywordsDiabetes, Endothelial cells, Epigenetics.


EngineeringPressure-induced magneto-structural transition in ironNikolai A. Zarkevich1, Duane D. Johnson1Ames Laboratory

AbstractThe magneto-structural transformations, including those of iron, have implications in materialsscience and engineering, metallurgy, geophysics, and planetary sciences. While experiments oftenuse constant-pressure (anvil cell) measurements, many theoretical results address a constant-volumetransitions, which avoid issues with magnetic collapse but cannot be directly compared to experiment.We establish a method to handle magnetic systems that may exhibit both moment and volume collapseduring transformation. We apply it to the pressure-induced transformation in iron between the low-pressure body-centered cubic (bcc) and the high-pressure hexagonal close-packed (hcp) phases, findthe bcc-hcp equilibrium coexistence pressure and a transitional pathway, and compare to shock andanvil cell experiments.

Poster IDP7.

KeywordsIron, Magnitostructural, Phase transformation, Pressure.


Graphene Oxide Decorated PEDOT Nanofibers for Sensing of Nitrate IonsMd Azahar Ali1, Huawei Jiang, Liang Dong1Department of Electrical and Computer Engineering – Iowa State University

AbstractWith the recent development in nanoscience and nanotechnology, functionalized conducting polymers-based nanofibers are found to have excellent performance for electrochemical biosensors due to theirlarger surface area, high porosity, and high conductivity. Conducting nanofibers offer versatile andunique properties for biomolecules/chemical species sensing. Here, we have fabricated a highlyselective and sensitive electrochemical biosensor using graphene oxide decorated conducting poly(3,4-ethylenedioxythiophene) (PEDOT-GO) with the use of a sacrificial carrier polymer for detection ofnitrate ions (NO3−). The PEDOT nanofibers mats on gold (Au) surface has been synthesized usingelectrospinning technique followed by vapor-phase polymerization [1]. The vapor-phase depositionof 3, 4-ethylenedioxythiophene can be transformed the oxidant nanofibers into quasi-pure PEDOTnanofibers. The average diameter of PEDOT fibers are around one micron and are linked at everyintersection of the mat, confirming a superior dimensional stability. The incorporation of GO sheetsinto the polymeric backbone of PEDOT can help to make covalent immobilization with nitratereductase enzyme. The high porosity and larger surface area of conducting PEDOT nanofibers canimprove the loading of GO sheets resulting in higher adsorption capacity of enzyme molecules. Thehigh aspect ratio nanofibers with GO sheets provide excellent sensing electrochemical characteristicsof NO3− ions. A high sensitivity (0.089 µA/µM/cm2) and low concentration (1.0 µM) of nitrate ionshave been observed using the fabricated PEDOT-GO biosensor compared to reported literature [2].Thus, PEDOT-GO conducting polymer could be a potential candidate in the development of electronicdevice for real-time monitoring of nitrate ions in plants.References[1] A. Laforgue, L. Robitaille, Macromolecules, 2010, 43, 4194-4200[2]. L. M. Moretto, P. Ugo, M. Zanata, P. Guerriero, C. R. Martin. Anal. Chem. 70, 1998, 2163-2166.

Poster IDP8.

KeywordsGraphene oxide, Nitrate, Nitrate biosensor, PEDOT.


GIS-based suitability model for siting bioethanol plants in the U.S.Bhavna Sharma1, F. E. Miguez1Department of Agronomy – Iowa State University

AbstractDue to the rising fuel prices and increasing energy demand, bioethanol has been recognized as a futurerenewable energy source. The goals and mandates developed for the renewable fuel production willrequire construction of several bioethanol plants throughout the U.S. Geographic Information Systems(GIS) suitability model was developed for identifying locations of bioethanol plants in the U.S. Thebiomass feedstocks considered for analysis was switchgrass (Panicum viragatum) and miscanthus(Miscanthus * giganteus). The spatial yield estimates and variability analysis combined with exclusionand preference GIS analysis was employed subject to different environmental, economic and socialfactors and constraints to identify potential sites. This national-scale assessment will enhance strategicdecision-making capabilities and understanding of spatial distribution of the biorefineries.

Poster IDP9.

KeywordsBioethanol, Biorefinery, Geographic Information Systems (GIS).


Agriculture and Life SciencesRecovering grass-infecting viruses in historical worldwide herbarium specimens tounderstand their evolution through timeJuliette Doumayrou1, Ellen Cole2,3, Mukta Dutta4, Ulrich Melcher4, W. Allen Miller1, CarolynMalmstrom3

1Dept. of Plant Pathology & Microbiology, Iowa State University, Ames, IA, 2Dept. of Biology, LoyolaUniversity Chicago, Chicago, IL, 3Dept. of Plant Biology, Michigan State University, East Lansing, MI,4Dept of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK

AbstractIn plant invasion ecology, parasites are often considered in terms of the enemy release hypothesis i.eplant invasion is promoted by escape from parasites. However, parasites may sometimes facilitatehost spread. Previous studies suggested that Barley and Cereal yellow dwarf viruses (Luteoviridae:B/CYDVs) could help historical European grasses invade and displace native grasses in California inthe 18th and 19th centuries, particularly if viruses were present during the invasion. The historicalviral RNA sequences from the 1920s were identified in invasive and native grasses and the firstphylogeny analysis on the viral coat-protein (CP) sequences supported this virus-assisted plantinvasion hypothesis (Malmstrom et al., 2007). To provide more evidence about historical ecological ofthese viruses and their rates of evolution, we investigated a worldwide collection of preserved historicalgrasses specimens at the University of California-Davis. These specimens were collected between1886 and 1981. In our analysis, we examined specimens from three time periods: (i) before 1900,the period in which shipping innovations made substantial increase in the rate of transoceanic traveland vector/pathogen movement; (ii) 1900-1940, a period from which we have previously recoveredviruses, and one that preceded the first identification of many virus species; and (iii) after 1940, aperiod for which the viruses epidemics are described. Viral RNA was extracted from all specimenswith care and amplified by primer-specific RT-PCR (Shu and Malmstrom, 2004; Malmstrom et al.,2007), then the CP gene was Sanger-sequenced for 17 positive specimens. To evaluate relationshipsamong preserved and worldwide present-day B/CYDVs, we constructed a first phylogeny analysisusing nucleotide sequences from the 17 historical sequences obtained in this project, and the 375B/CYDVs available in Genbank. The first results will be presented in this poster.

Poster IDP10.

KeywordsEcology, Evolution, Grass, Herbarium, Luteovirus.


Structure and evolution of the youngest sex chromosome system in turtlesEugenia E. Montiel Jimenez1, R. Literman, D. Badenhorst, J. Tamplin, R. Burke, N. Valenzuela1Department of Ecology, Evolution and Organismal Biology – Iowa State University

AbstractTurtles vary in their sex-determining mechanism. Most turtle species possess temperature-dependentsex determination (TSD) but genotypic sex determination (GSD) has evolved multiple times inde-pendently from the TSD ancestral condition. Most GSD in animals involves sex chromosomes,yet the sex chromosome system of only 9 out of 22 GSD turtles has been characterized. Here wepresent a study in Glyptemys insculpta, a GSD turtle in the family Emydidae representing one ofthe youngest sex chromosome systems known in chelonians. Data obtained from a combinationof comparative genome hybridization (CGH), BAC clone in situ hybridization (BAC FISH), andlow-coverage genome sequencing, allowed us to characterize the sex chromosome system in thisspecies (to distinguish whether it is XX/XY or ZZ/ZW), and provide insight into the structure, originand evolution of the sex chromosomes in G. insculpta. Chromosomal rearrangements were identifiedin the sex chromosome pair that may have mediated their divergence, and candidate genes involved insexual development were located to this genomic region that may have play a critical role in GSDevolution in this turtle species.

Poster IDP11.

KeywordsBAC clone in situ hybridization (BAC FISH), Comparative Genome Hybridization (CGH), Evolution,Sex chromosomes, Turtle.


Effects of co-products of enzyme-assisted aqueous extraction of soybeans on ethanolproduction in corn fermentationJasreen K. Sekhon1, K. Rosentrater2, T. Wang1, L. Johnson1, S. Jung1,3

1Iowa State University Department of Food Science and Human Nutrition, Ames IA USA and Centerfor Crops Utilization Research, Ames IA , USA, 2Iowa State University Department of Agricultural andBiosystems Engineering, Ames IA, USA, 3California Polytechnic State University, San Luis Obispo,Department of Food Science and Nutrition, CA, USA

AbstractBiofuels can reduce dependency on non-renewable sources of fuels and offer multiple environmentalbenefits. However, more efficient methods of biofuel production are needed to effectively competewith petroleum fuels in the market place. One such approach is an integrated corn-soy biorefinery,which combines components from soybean processing into corn-based ethanol processing. Enzyme-assisted aqueous extraction processing (EAEP) is an environmentally-friendly alternative to chemicaland mechanical extraction methods which facilitate 97% oil recovery from soybeans. The presentstudy utilized soy skim (protein rich) and insoluble fiber (IF; carbohydrate rich), co-products of EAEP,in dry-grind corn fermentation, and investigated the effect of adding 1) soy skim and IF separately ortogether, and 2) pretreated or untreated IF on ethanol production. Maximum ethanol production wasachieved when corn, untreated IF and skim were slurried together (corn-to-untreated IF ratio 1:0.2;skim-to-untreated IF ratio 4:1, on dry basis) and fiber hydrolyzing enzymes were added. This resultedin 20% and 3% increase in ethanol yield and ethanol production rate, respectively, and a 44 h decreasein fermentation time compared to corn-only fermentation.

Poster IDP12.

KeywordsBioethanol, Corn fermentation, Enzyme-assisted aqueous extraction of oil, Soybean.


Chemistry and BiochemistryGenotypic Diversity of Streptococcus sobrinus in American Indian ChildrenDavid Lynch1, A. Villhauer, D. Dawson, J. Warren, T. Marshall, K. Phipps, D. Starr, D. Drake1College of Dentistry – University of Iowa

AbstractObjectives: Severe early childhood caries (SECC) is a debilitating form of tooth decay and isprevalent among lower socioeconomic groups. American Indian children exhibit some of the worsttooth decay of all socioeconomic groups. Streptococcus mutans (SM) and Streptococcus sobrinus (SS)are major etiologic agents in SECC. While SS is less common than SM, it is more closely associatedwith high caries activity. We are conducting a study focusing on the transmission of SM and SSin American Indian children from a Northern Plains Tribe with rampant caries. Here, we report onpreliminary analysis of genotypic diversity of SS colonization in 36 month old children from thisstudy.Methods: Whole mouth plaque samples were collected from 36 month old American Indian childrenfrom a subset of our cohort. Plaque samples were cultured on selective agar to isolate SM and SScolonies. DNA was then extracted from putative SM or SS for species identification by PCR andthe SS isolates were then genotyped by AP-PCR. Gel images, from AP-PCR were analyzed anddendograms were created using GelComparr IIv6.5 gel analysis software.Results: Approximately 93% of children and harbor SM, SS or both species and roughly 30-35% ofall isolated mutans streptococci colonies are SS. Streptococcus sobrinus isolates were recovered fromchildren as young as 12 months. Preliminary results show that there are multiple SS genotypes in thispopulation.Conclusions: While plaque is a highly complex microbial community and caries is a multifactorialprocess, the presence of SS could play a vital role in SECC that is rampant in this population. Morecomprehensive analyses of genotypic diversity, commonality and stability of SS in children, mothersand caregivers, as well as, the relationship between SS and caries are currently underway.

Poster IDP13.

KeywordsCaries, Genotypes, Mutans streptococci, Streptococcus.


Exploiting Soft Template Synthesized Ordered Mesoporous Carbons for CatalysisNaftali N. Opembe1, Zhuoran Wang, Nicholas C. Nelson, Takeshi Kobayashi, Marek Pruski, Igor I.Slowing1Ames Laboratory

AbstractOrdered mesoporous carbons (OMC) are attractive materials owing to their innate inert structure,resistance towards acidic or basic media, low cost, and good recycleability. We explore the synthesisof OMC via soft templating means with keen interest in exploring their framework composition andthe types of groups present. The knowledge of the composition allows us to make unambiguousdesign of OMC-based catalysts.

Poster IDP14.

KeywordsHeterogeneous catalysis, Ordered mesoporous carbons, Soft-template synthesis.


Rapid spread of measles virus in primary human airway epitheliaBrajesh K. Singh1, Andrew L. Hornick, Sateesh Krishnamurthy, Anna C. Locke, Crystal A.Mendoza, Mathieu Mateo, Catherine L. Miller-Hunt, Roberto Cattaneo and Patrick L. Sinn1University of Iowa

AbstractThe discovery that measles virus (MV) uses the adherens junction protein nectin-4 as its epithelialreceptor provides a new vantage point from which to characterize its rapid spread in the airwayepithelium. We show here that in well-differentiated primary cultures of airway epithelial cells fromhuman donors (HAE), MV infectious centers form rapidly and become larger than those of the otherrespiratory pathogens: human respiratory syncytial virus, parainfluenza virus 5, and Sendai virus.While visible syncytia do not form after MV infection of HAE, the cytoplasm of an infected cellsuddenly flows into an adjacent cell, as visualized through wild type MV-expressed cytoplasmic greenfluorescent protein (GFP). High-resolution video microscopy documents that GFP flows throughopenings that form on the lateral surfaces between columnar epithelial cells. To assess the relevanceof the protein afadin, which connects nectin-4 to the actin cytoskeleton, we knocked down its mRNA.This resulted in more limited infectious center formation. We also generated a nectin-4 mutant withoutthe afadin-binding site in its cytoplasmic tail. This mutant was less effective than wild-type humannectin-4 in promoting MV infection in primary cultures of porcine airway epithelia. Thus, in airwayepithelial cells, MV spread requires the nectin-4/afadin complex, and is based on cytoplasm transferbetween columnar cells. Since the viral membrane fusion apparatus may open the passages that allowcytoplasm transfer, we refer to them as intercellular membrane pores. Virus-induced intercellularpores may contribute to extremely efficient measles contagion by promoting rapid spread of the virusthrough the upper respiratory epithelium.

Poster IDP15.

KeywordsAirways, Measles, Spread.

28 Abstracts - Oral Presentations

Oral PresentationsTeaching and Human BehaviorTeaching as Research: Understanding students’ perceptions and knowledge of aninterdisciplinary engineering courseBhavna Sharma1, B. Steward, S. K. Ong, F. E. Miguez1Department of Agronomy – Iowa State University

AbstractSustainable Engineering and International Development (SEID) is an interdisciplinary course focusedon describing the key concepts of sustainability and sustainability engineering in the global contextand its requirement in the developing countries. Teaching an interdisciplinary engineering courseis challenging due to the diverse background and discipline-specific skill set of students. In thepresent study, we investigate students’ perceptions and knowledge attained in the SEID course, toidentify the areas of improvement. The assessment techniques used were pre-and post-test, focusgroup discussions, and survey. The difficulty level pre-and post- test was found to be low, indicatingthat students have foundational/basic knowledge of sustainability. Therefore, emphasis should be onteaching application of sustainability engineering across systems discussed in the lecture modules.Focus group discussions and survey results showed that students liked modular format of the course,guest lectures, and team-projects. In order to enhance project-based learning in interdisciplinaryteams, access to additional material/lectures on topics related to the projects was recommended. Inaddition, students also suggested re-ordering the lecture modules; focusing more on the in-classproblem solving; introducing topics related to sustainability in the context of the developed countries;and providing entire laboratory class hours for students to work on their team projects. The outcomesfrom this assessment will help in improving the teaching-learning process for this course and similarstrategy could be followed for the enhancement of other interdisciplinary courses.

Presentation IDO1.

KeywordsEducation, Engineering, Sustainability.


Teaching biodiversity positively influences both the cognitive and affective domainsCarol L. Chaffee1, Glené Mynhardt, James T. Colbert1Iowa State University

AbstractIn Fall, 2012, Iowa State University implemented an inquiry-based, discovery-driven approach to theexploration of biodiversity in the first semester of introductory biology. Thus, a primary motivationfor this project was to evaluate student attitudes towards and understanding of biodiversity under thisnew approach. We used surveys of both student attitudes and conceptual understanding to assesshow this new approach influenced both the affective and cognitive domains in relation to biodiversity.Surveys of student attitudes were administered both before (Spring, 2012) and after (Fall, 2012,Spring, 2013) the implementation of the discovery-driven approach. Tests assessing biodiversityconceptual knowledge were administered both prior to and immediately following the biodiversityportion of lab in the Spring and Fall, 2013. Our results show that fewer than half of our studentsenter the introductory biology course believing they have a good knowledge of biodiversity–a resultsupported by low scores on the conceptual pre-test. While attitudes toward biodiversity showed nosignificant change during the course, student confidence in their biodiversity knowledge increasedsignificantly, and scores on the conceptual post-test indicate this confidence is warranted. Theseresults indicate that the inquiry-based, discovery-driven approach significantly improves both theunderstanding of biodiversity, and student confidence in their knowledge of biodiversity.

Presentation IDO2.

KeywordsBiodiversity, Biology education, Student attitudes.


Naturalistic study of adult and child bicycling behaviors and risk exposureCara Hamann1

1University of Iowa

AbstractThe purpose of this study was to naturalistically examine bicycling behaviors and risks by age andgender. We conducted a naturalistic, first-hand, bicycling study of 10 children (aged 10 to 14) and 10adults. Participants were asked to record all of their bicycling trips, via a GPS-enabled helmet cameraand trip diaries, for one week. Eligible participants lived in Johnson County, Iowa, and regularly rodetheir bicycle. Data were manually reviewed and coded to identify and calculate rates of safety-criticalevents (bicyclist and driver errors, near crashes, and crashes) and bicycle trip characteristics (route,distance, time of day, sidewalk riding, and use of on and off-street bicycle facilities). Video and GPSdata were captured for 283 bicycle trips (57 hours, 670 miles). During the one week study period,boys took more trips than girls (Mean trips: 12.6 vs. 8.8) but trip lengths were similar (Mean milesper trip: 1.1 vs.1.3). Male and female adults took a similar number of trips (Mean: 15.2 vs. 15.6), butmales had longer trips (Mean miles per trip: 5.0 vs. 2.0; p=0.01). Children had lower error rates thanadults (0.13 vs 0.31 per mile), but they rode more often on the sidewalk (56.4% vs 12.7%; p<0.01),where traffic rules apply less frequently.Conclusions: The use of GPS-enabled helmet cameras is an effective tool for capturing bicyclingexperiences first-hand. Data captured in this study revealed large variations between both adults andchildren and males and females in bicycling behaviors. Data from this study are unique, as they arethe first naturalistic cycling data collected among children and in the United States. Results begin toreveal differences by age and gender in bicycling safety and use of the transportation system, whichcan inform both intervention development and infrastructure design.

Presentation IDO3.

KeywordsAdult, Bicycle, Child, Naturalistic, Safety.


Agriculture and Life SciencesPolypyrimidine tract-binding proteins regulate RNA metabolism of a mobile RNAfor potato tuberizationSung Ki Cho1, Pooja Sharma, Il-Ho Kang, David Hannapel1Dept of Plant Pathology and Microbiology – Iowa State University

AbstractPolypyrimidine tract-binding (PTB) proteins are a family of RNA-binding proteins that regulatevarious RNA metabolisms via binding to motifs with cytosines (C) and uracils (U). Potato genomehas six PTB proteins, and two of these are identified as RBP50-types, which were studied as thecore protein of a mobile RNA/protein complex, and named as StPTB1 and StPTB6. in vivo and invitro RNA-binding assays showed the interaction of StPTB proteins with CU-rich sequences of the3’ untranslated regions of the phloem-mobile mRNA, StBEL5, which has been characterized as animportant signaling molecule for tuberization of potato. RNA accumulation of both StPTBs wasinduced by short days in leaves in correlation with enhanced accumulation of StBEL5 RNA in stolons.StPTB suppression lines exhibited reduced tuber yields and decreased StBEL5 RNA accumulation,whereas StPTB over-expression lines displayed an increase in tuber production correlated with theenhanced production of steady-state levels of StBEL5 and GA2 oxidase1, which is the tuber identitygene, in stolons. Assays for stability and long-distance transport of the mobile StBEL5 RNA weresignificantly enhanced from over-expression lines, and decreased from StPTB suppression lines. Basedon these analyses, we propose that these RNA-binding proteins play an important role in regulatingRNA metabolisms that impact tuberization of potato through an interaction with phloem-mobiletranscripts of StBEL5.

Presentation IDO4.

KeywordsLong-distance signal, Potato tuberization, RNA-binding protein.


Estimating dry matter and composition loss due to rainfall during field drying ofswitchgrass and corn stoverAmit Khanchi1, Stuart Birrell1Iowa State University

AbstractAn experimental study was conducted to estimate the impact of rainfall amount and crop density oncompositional and dry matter loses from switchgrass and corn stover. Three levels of densities werestudied for both switchgrass and corn stover. During the experiments, switchgrass dry density variedfrom 0.97 kg m−2 to 3.23 kg m−2 and corn stover dry density varied from 0.78 kg. m−2 to 2.61 kg.m−2. Switchgrass and corn stover samples were subjected to four amounts of rainfall amount rangingfrom 0.3 in to 3 in under a rainfall simulator. After the rainfall treatment, both switchgrass and cornstover samples were incubated for 48 hours at room condition to simulate the field drying period aftera rainfall event. A factorial design was considered with three replications at each rainfall amount. Allexperiments were conducted at a moderate rainfall intensity of 0.3 in hr−1. Losses from both cornstover and switchgrass were measured by following methods: measuring the total solids in the runoffwater after a rainfall event (leaching loss), extractive loss by measuring the amount of water solubleportion of biomass before and after rainfall events and measuring the dry matter in the biomass samplebefore and after the rainfall treatment using the oven drying method (dry matter loss). Before andafter rainfall treated samples were also subjected to mineral, ash, and fiber content analysis.

Presentation IDO5.

KeywordsDry matter loss, Mineral change, Rainfall loss.


Effects of maternal behavior and nutrition on caste-biased gene expression duringlarval development in Polistes waspsJennifer Jandt11Iowa State University

AbstractCaste differences in most social insects result from differences in environmental input, such asnutrition and exposure to pheromones. Recent evidence for Polistes wasps suggests an additionalsocial input– maternal vibrational signaling– influences the probability that a larva becomes a workeror a queen as an adult. Maternal females frequently perform “antennal” drumming on the nest,and larvae subjected to this drumming develop into adults with low, worker-like fat stores. Wehypothesized that maternal drumming behavior leads to worker-biased gene expression patterns indeveloping larvae. We paired this with a nutritional manipulation to examine the interaction betweenvibrational and nutritional inputs during development. To assess the effects of nutrition and drummingon caste development in Polistes fuscatus, we performed a field experiment in which nests weresubjected to artificially simulated drumming and a nutritional manipulation (via foraging restrictions).Half the nests received simulated drumming at a constant, low vibrational frequency; whereas theother half received no drumming. Half of each drumming group was subjected to restricted foragingeach morning (foragers could not leave the nest until mid-day) to simulate low-nutrition conditions,while the other half of the nests were not restricted, to simulate normal nutrition conditions. Wepredicted that larvae that experienced vibrations that simulated drumming + low-nutrition conditionswould exhibit worker-like gene expression of caste biomarker genes related to fat metabolism andheat shock, compared to those that receive no drumming and normal nutrition conditions. Our resultsprovide the first transcript level test that maternal vibrational signals, in combination with nutritionalinputs, can influence caste development via shifts in gene expression during development.

Presentation IDO6.

KeywordsEusocial wasp, Gene expression, Nutrition.


Chemistry and MathematicsRhodium-Catalyzed Regioselective Hydroacylation of Alkynes and Its Applicationin the Total Synthesis of Isoflavones: Daidzein and FormononetinXiang-Wei Du1

1Department of Chemistry – Iowa State University

AbstractTrimethylsilyl group was utilized as a controlling group to achieve highly regioselective hydroa-cylation of asymmetrical alkynes. A broad scope of alkynes with aryl and alkyl substitutes wereamenable to this strategy. After simple desilylation, the branched products were obtained easily. Thesynthetic utility of our method has been demonstrated in the efficient total synthesis of daidzein andformononetin.

Presentation IDO7.

KeywordsRegioselective hydroacylation.


Probing the chemistry and properties of sea spray aerosol using single particle mi-croscopy and spectroscopy techniquesArmando D. Estillore1, Olga Laskina, Joshua R. Grandquist, Jonathan V. Trueblood, Vicki H.Grassian1University of Iowa

AbstractSea spray aerosol (SSA) is a major source of natural aerosol particles in the atmosphere. These aerosolparticles are composed of a complex mixture of inorganic and organic components. Understanding therole and impact of SSA on climate requires investigation on its chemical properties and its interactionwith water. We are using a multi-faceted approach in investigating the complex chemistry of SSAparticles collected from the 2014 Investigation into Marine Particle Chemistry and Transfer Science(IMPACTS) campaign in the Center for Aerosol Impacts on Climate and the Environment (CAICE)Ocean-Atmosphere Facility in San Diego, CA (http://caice.ucsd.edu/). We used two spectrochemicalimaging and microanalysis techniques: electron microscopy (EM) coupled with energy dispersive X-ray spectroscopy (EDX) to probe the size and morphology of individual substrate deposited particles aswell as spatially resolved elemental information and micro-Raman spectroscopy to study the chemicalcomposition of the individual SSA particles. The Raman microscope equipped with environmentalcell was also used to study the water uptake of collected particles. Results of these experiments willbe presented and discussed.

Presentation IDO8.

KeywordsRaman spectroscopy, Scanning electron microsopy, Sea spray aerosol.


Elastic collisions among peakon solutions for the Camassa-Holm equationTerrance Pendleton1, Alina Chertock, Jian-Guo Liu1Iowa State University

AbstractIn this talk, we study the dynamics of the interaction among a special class of solutions of theone-dimensional Camassa–Holm equation. The equation yields soliton solutions whose identity ispreserved through nonlinear interactions. These solutions are characterized by a discontinuity atthe peak in the wave shape and are thus called peakon solutions. We apply a particle method tothe Camassa–Holm equation and show that the nonlinear interaction among the peakon solutionsresembles an elastic collision, i.e., the total energy and momentum of the system before the peakoninteraction is equal to the total energy and momentum of the system after the collision. From thisresult, we provide several numerical illustrations which support the analytical study, as well asshowcase the merits of using a particle method to simulate solutions to the Camassa–Holm equationunder a wide class of initial data.

Presentation IDO9.

KeywordsCamassa–Holm equation, Conservation laws, Elastic collisions, Particle method, Peakon solutions.


Materials Science and EngineeringParticles for joining and manufacturing of metals at room temperatureSimge Cinar1, Ian D. Tevis, Jiahao Chen, Martin Thuo1Material Science and Engineering – Iowa State University

AbstractJoining is an integral part of manufacturing in many industries such as electronics, chemical, energy,medical, aerospace, defense and many others, but has been facing challenges because of ever-advancing miniaturization and need for green processes. Current techniques for nano-scale joining,however, suffer from low efficiency, high costs and require specialized samples, so they are far frombeing adapted in large scale manufacturing. For micron systems, on the other hand, practical, energyefficient joining and manufacturing technique with low processing temperatures is essential. Wesuggested to use of encapsulated undercooled nano- or micro- particle as manufacturing platformor a low temperature solder. We synthesized stable undercooled metal particles using the SLICE,an extension of the droplet emulsion technique, and demonstrated the stable particulate engineeringat room temperature. Instances include (i) fabricating complex structures formed by removal ofouter layers using focused ion beam milling to form 3D structures that are otherwise difficult tofabricate; (ii) joining and manufacturing of complex shapes at ambient conditions through mechanicalstressing and breaking of the outer layers of particles, and subsequent coalescence and instantaneoussolidification of the undercooled metal.

Presentation IDO10.

KeywordsDroplet emulsion technique, Joining, Manufacturing, Particulate engineering, Undercooling.


Concentration waves in high-entropy alloys – a novel alloy design approachPrashant Singh1, Andrei Smirnov, D. D. Johnson1Ames Laboratory

AbstractChemical short-range order (SRO) in solid solutions can be interpreted as a “concentration wave”- a Fourier decomposition of nascent order - identified experimentally via Warren-Cowley SROparameters. We present a rigorous thermodynamic theory to predict and uniquely interpret the SROin N−component alloys. Based on KKR-CPA electronic structure, we implemented this methodusing thermodynamic linear-response to include all alloying effects, e.g., band-filling, hybridization,Fermi−surface nesting and van Hove instabilities. We apply this first-principles method to high-entropy alloys (HEAs), i.e., solid solutions with N≥5 that inhibit small-cell order due to large entropycompeting against ordering enthalpy, as their properties are sensitive to SRO. We validated theory withcomparison to experiments in A2 Nb-Al-Ti. We then predict and analyze SRO in Co-Cr-Cu-Fe-Niand Co-Cr-Al-Fe-Ni systems - showcasing this new first-principles-based alloy design method.

Presentation IDO11.

KeywordsConcentration wave, High entropy allloy, Short range order (SRO).


Cleaning of biomass-generated syngas for production of biofuels and biochemicalsAshokkumar M. Sharma1, Lysle Whitmer, Patrick A. Johnston, Robert C. Brown1Bioeconomy Institute (BEI) – Iowa State University

AbstractThe gasification process converts biomass (such as perennial grasses and agro-residues) into syngas,a mixture primarily composed of hydrogen and carbon monoxide which can serve as a buildingblock for many fuels and chemicals. One promising research area within the biomass sector ishybrid processing, in which a thermochemical process (gasification) is combined with a biochemicalprocess (fermentation) in order to utilize microorganisms to digest syngas and produce renewablealcohols or chemicals. However, biomass-derived syngas contains an unavoidable impurity called tar,which adversely affects the efficiency and economy of the downstream syngas conversion processesincluding fermentation. The goal of the present study was to design, develop and evaluate a solvent-based scrubber system for removal of light tar compounds such as benzene, which inhibit and/orkill syngas-fermenting microorganisms. A light tar cleanup system, which consisted of two 2 in.scrubber columns, was designed, developed and experimented using both a simulated syngas streamcomprising of benzene as a light tar compound, and a real syngas slip-stream from an existingpilot-scale fluidized-bed biomass gasification system. Performance tests using nonpolar (n–hexaneand synthetic oil) and polar (n–butanol) solvents showed very promising results with 100% removalof benzene from both simulated and real syngas streams.

Presentation IDO12.

KeywordsBenzene, Biomass gasification, Light tar, Syngas cleaning, Syngas fermentation.


Identification of umbrella constraints in DC-based security-constrained optimal powerflowAli Jahanbani Ardakani1, Francois Bouffard1Iowa State University

AbstractSecurity-constrained optimal power flow (SCOPF) problems are essential tools to transmission systemoperators for long-term and operational planning and real-time operation. The general goal of SCOPFproblems is to optimize electricity network operation while ensuring that operational and planningdecisions are consistent with technical limits under both pre- and post-contingency states. Thesolution of SCOPF problems is challenging because of the inherent size and scope of modern grids.As empirical evidence and longstanding operator experience show, relatively few of the constraintsof SCOPF problems actually serve to enclose their feasible region. Hence, all those constraints notcontributing directly to set up the SCOPF feasible space are superfluous and could be discarded. Inlight of this observation, this paper proposes an optimization-based approach for identifying so-calledumbrella constraints in SCOPF problems where the network operation is approximated by the dcpower flow. Umbrella constraints are constraints which are necessary and sufficient to the descriptionof the feasible set of an SCOPF problem. The resulting umbrella constraint discovery problem (UCD)is a convex optimization problem with a linear objective function. For SCOPF problems of practicalimportance, the UCD is also quite large and requires the use of a decomposition technique. In thispaper, we concentrate on an SCOPF formulation for preventive security generation dispatch. We showthat by removing superfluous constraints, the resulting sizes of SCOPF problems are much smallerand can be solved significantly faster.

Presentation IDO13.

KeywordsComputational complexity, Convex optimization, Generation dispatch, Linear programming, Security-constrained optimal power flow (SCOPF), Umbrella constraint.


Biomedicine, Veterinary Medicine and Molecular BiologyActivation of the innate immune response upon mitochondrial dysfunction protectsagainst neurodegeneration in C. elegansMadhusudana Rao Chikka1

1University of Iowa

AbstractThe role of the innate immune response in neurodegenerative diseases remains unclear. Usinga C. elegans model of rotenone-induced dopaminergic neurodegeneration, we show that rotenonetreatment, or dysregulation of mitochondrial Complex I, triggers the innate immune response throughp38 MAPK-dependent activation of the CREB/ATF transcription factor, ATF-7. This activation of theinnate immune response is protective: downregulating p38 MAPK or ATF-7 exacerbates rotenone-induced neurodegeneration. In contrast, overexpressing active, but not dead p38 MAPK kinase inperipheral tissue alone is sufficient to protect animals from rotenone-induced neurodegeneration,through the activation of ATF-7. ATF-7-mediated innate immune response protects against rotenone-induced neurodegeneration by stimulating autophagy. We propose that the activation of the p38MAPK dependent innate immune response upon mitochondrial dysfunction is part of a mitochondrialsurveillance mechanism, whereby disruption of mitochondrial complex I triggers the clearance ofdysfunctional mitochondria by mobilizing autophagy, thereby maintaining homeostasis.

Presentation IDO14.

KeywordsInnate immune response, Mitochondria, Neurodegeneration.


Use of computerized 3-D motion analysis as a biomechanical assessment tool inbiomedical studies in-vivoSina Safayi1, Nick D. Jeffery1Department of Veterinary Clinical Sciences – Iowa State University

AbstractAnimals are used in in-vivo studies because they have a response similar to humans that cannot bereplicated in a non-animal model. Animals not only have similar electrophysiological characteristics tohumans, but also are excellent test beds for evaluating therapies such as electromechanical robustnessof medical devices affecting motion; e.g. biomechanics of limbs. As an assessment tool, motioncapture has been widely used in preclinical as well as clinical applications in biomedical sciences toquantify functional movement limitations and biomechanics of a subject in-vivo. In order to showhow image based 3-D motion computer system could be employed in biomedical studies, we willdiscuss how such biomechanical assessment tool works and would be employed not only to evaluatethe safety of therapies but also to track their possible improvements in-vivo. In particular, we havechosen to review some of the ongoing collaborative multidisciplinary research projects in Midwestregion USA.

Presentation IDO15.

KeywordsBiomechanics, Biomedical, Kinematics, In-vivo, Motion analysis.


Determination of the concentration of tylvalosin in the synovial fluid of swine afteradministration at a dose of 5mg/kg by oral gavagePaisley Canning1, Locke Karriker1, Jessica Bates1, Kristin Hammen1 Suzanne Sander, JohannCoetzee1

1Veterinary Diagnostic and Production Animal Medicine – Iowa State University

AbstractInfectious arthritis is a welfare and economic concern for swine operations. Veterinarians report thatthe antibiotic tylvalosin is effective against common bacterial causes of arthritis. There is limited dataon the efficacy of tylvalosin (TVN) for arthritis or its distribution to the joint (synovial) fluid. Thisstudy determines if tylvalosin and its metabolite, 3-O-acetyltylosin (3AT) distributes to the synovialfluid of pigs when administered at 5mg/kg by oral gavage and if TVN and 3AT remain stable insynovial fluid when stored for 28 days at -70◦C.24, 45kg pigs were orally gavaged with 5mg/kg of TVN and randomly allocated to one of 6 treatmentgroups. Treatment group referred to the time point (0, 2, 4, 6, 8 or 10 hours post-gavage) at which thepigs were euthanized and synovial fluid was collected. For each pig, synovial fluid was collected andsplit into four parts. Aliquots 1 to 4 were analyzed at 0, 7, 14 and 28 days storage at -70◦C. Sampleswere analyzed for TVN and 3AT by high pressure liquid chromatography and mass spectrometry. AGLIMMIX procedure on SAS 9.4 was run for log concentration.TVN and 3AT were detected in synovial fluid samples from all treatment groups except at hour 0.Mean peak concentrations were 31.17 ng/mL (95% CI: 18.62 – 52.16) for TVN and 58.82 ng/mL(95% CI: 35.14 – 98.46) for 3AT. Peak concentrations occurred at 2 hours post-gavage. Storage timedid not impact the concentration of TVN and 3AT in the samples (p-value 0.9732).Concentrations of 3AT and TVN in the joint can be compared to minimum inhibitory concentrations(MICs) for bacteria associated with arthritis to guide the judicious use of antimicrobials. Clinicalcorrelations to MICs have not been documented. The dosing approach used here is dissimilar fromfarm administration where tylvalosin is dispensed via water lines. The dosing strategy may affect thepeak concentrations attained in synovial fluid and drug efficacy interpretations based on these values.

Presentation IDO16.

KeywordsAntibiotics, Macrolides, Pharmacology, Swine.


The effect of CX-4945, a CK2 inhibitor, on cholangiocarcinoma (CCA): in vitro data

Kais Zakharia1, Catherine D. Moser, Mitesh J. Borad, Lewis R. Roberts1Mayo Clinic

AbstractBackground: There is a need to develop effective therapies for CCA due to the suboptimal outcomeswith the current standard gemcitabine (Gem) and cisplatin (Cis) therapy. Casein kinase II (CK2) isa serine/threonine selective protein kinase that has been implicated in cancer progression making itan attractive target for anticancer treatment. CX-4945 is a first in class, orally available and highlyselective inhibitor of CK2 protein kinase. CX-4945 has shown antitumor activity in other humancancers. In this study we investigated the anti-neoplastic effect of CX-4945 on 3 different CCA cells.Methods: CCK8 and colony formation assays were used to assess cell viability in cultures of HuCCT1,EGI-1, and the new primary patient derived CCA cell line LIV27 after treatment with CX-4945 aloneor in combination with Gem, Cis or both. The half maximal inhibitory concentrations (IC50) werecalculated using the Chou-Talalay technique. Western immunoblotting was used to assess the effectof CX-4945 on Akt pathway in CCA cells.Results: CX-4945 significantly decreased cell viability of HuCCT1, EGI-1 and LIV27 in a timedependent and a dose dependent manner (with IC50 of 7.3, 9.5, and 9.4 µM respectively at 72 hours).CX-4945 inhibited colony formation in HuCCT1 and EGI-1 with IC50 of 3.8 and 1.6 µM respectively.CX-4945 inhibited Akt phosphorylation at two different phosphorylation sites (Serine473 and Ser-ine129) suggesting that it acts through the Akt pathway. CX-4945 had a synergistic (if higher dosesused) or additive effect (if lower doses used) when combined with either Cis or Gem on HuCCT1,EGI-1 and LIV27 cells. Addition of CX-4945 to both Gem and Cis together ( 1

4 IC50s, 12 IC50s)

further decreased viability of HuCCT1 cells.Conclusion: CX-4945 has an anti-proliferative effect on CCA. It works through inhibiting Aktpathway. CX-4945 enhanced the anti-proliferative effect of both Cis and Gem in vitro. Additionaldata are needed to validate these findings

Presentation IDO17.

KeywordsAkt pathway, Cholangiocarcinoma, CK2 inhibitor, CX-4945, HuCCT1.

List of presenters

Ali Md Azahar (poster), 2, 20Ardakani Ali Jahanbani (oral), 4, 40

Blue Emily K. (poster), 2, 18

Canning Paisley (oral), 4, 43Chaffee Carol L. (oral), 3, 29Chikka Madhusudana Rao (oral), 4, 41Cho Sung Ki (oral), 3, 31Cinar Simge (oral), 4, 37

Doumayrou Juliette (poster), 3, 22Du Xiang-Wei (oral), 3, 34

Estillore Armando D. (oral), 4, 35

Gutman Andrea (poster), 2, 13, 17

Hamann Cara (oral), 3, 30

Jandt Jennifer (oral), 3, 33

Khanchi Amit (oral), 3, 32

Kolb Ryan (poster), 2, 16

Lynch David (poster), 3, 25

Madden Jill A. (poster), 2, 15Montiel Jimenez Eugenia E. (poster), 3, 23

Opembe Naftali N. (poster), 3, 26

Pendleton Terrance (oral), 4, 36

Safayi Sina (oral), 4, 42Sekhon Jasreen K. (poster), 3, 24Sharma Ashokkumar M. (oral), 4, 39Sharma Bhavna (oral), 3, 28Sharma Bhavna (poster), 3, 21Singh Brajesh K. (poster), 3, 27Singh Prashant (oral), 4, 38

Zakharia Kais (oral), 4, 44Zarkevich Nikolai A. (poster), 2, 19Zbarska Svitlana (poster), 2, 14

List of keywords

Addiction, 13Adult, 30Advocacy, 17Airways, 27Akt pathway, 44Angiogenesis, 16Antibiotics, 43ASICs, 13

BAC clone in situhybridization(BAC FISH), 23

Benzene, 39Bicycle, 30Biodiversity, 29Bioethanol, 21, 24Biology education, 29Biomass gasification, 39Biomechanics, 42Biomedical, 42Biorefinery, 21Bone, 14

Camassa–Holm equation,36

Cancer, 16Cancer therapy, 15Caries, 25

Child, 30Cholangiocarcinoma, 44CK2 inhibitor, 44Cocaine, 13Comparative Genome

Hybridization(CGH), 23

Computational complexity,40

Concentration wave, 38Conservation laws, 36Convex optimization, 40Corn fermentation, 24CX-4945, 44

Diabetes, 18Droplet emulsion

technique, 37Dry matter loss, 32

Ecology, 22Education, 28Elastic collisions, 36Endothelial cells, 18Engineering, 28Enzyme-assisted aqueous

extraction of oil,24

Epigenetics, 18Eusocial wasp, 33Evolution, 22, 23

FoxM1, 15Funding, 17

Gene expression, 33Gene regulation, 15Generation dispatch, 40Genotypes, 25Geographic Information

Systems (GIS), 21Graphene oxide, 20Grass, 22

Herbarium, 22Heterogeneous catalysis,

26High entropy allloy, 38HuCCT1, 44

In-vivo, 42Inflammation, 16Innate immune response,

41Iron, 19

Joining, 37

47 List of keywords

Kinematics, 42

Light tar, 39Linear programming, 40Long-distance signal, 31Luteovirus, 22

Macrolides, 43Magnitostructural, 19Manufacturing, 37Measles, 27Mineral change, 32Mitochondria, 41Motion analysis, 42Mutans streptococci, 25

National Institutes ofHealth (NIH), 17

National ScienceFoundation(NSF), 17

Naturalistic, 30Neurodegeneration, 41Nitrate, 20Nitrate biosensor, 20NLRC4, 16

Nucleus accumbens, 13Nutrition, 33

Obesity, 16Ordered mesoporous

carbons, 26Ovarian cancer, 15

Particle method, 36Particulate engineering, 37Peakon solutions, 36PEDOT, 20Peripheral nerve

regeneration, 14Pharmacology, 43Phase transformation, 19Policy, 17Potato tuberization, 31Pressure, 19

Rainfall loss, 32Raman spectroscopy, 35Regioselective

hydroacylation,34

RNA-binding protein, 31

Safety, 30Scanning electron

microsopy, 35Schwann cells, 14Sea spray aerosol, 35Security-constrained

optimal powerflow (SCOPF), 40

Sex chromosomes, 23Short range order (SRO),

38Soft-template synthesis, 26Soybean, 24Spread, 27Streptococcus, 25Student attitudes, 29Sustainability, 28Swine, 43Syngas cleaning, 39Syngas fermentation, 39

Turtle, 23

Umbrella constraint, 40Undercooling, 37

Symposium Organizers

TIM BRENZADepartment of Chemical and Bi-ological Engineering, Iowa StateUniversity [email protected]

PRINCE AGBEDANUDepartment of Biomedical Sci-ences, Iowa State [email protected]

SVITLANA ZBRASKADepartment of Gen., Dev. andCell Biol., Iowa State [email protected]

ELKE BRANDESDepartment of Agronomy,Iowa State [email protected]

EMILIE GUIDEZDepartment of Chemistry,Iowa State [email protected]

JULIETTE DOUMAYROUDepartment of Plant Pathologyand Microbiology, Iowa StateUniversity [email protected]

50 Symposium Organizers

HARRIS LINAmes Lab’s cyber secu-rity group, Ames [email protected]

JOSE MARIA YUSTE RO-DRIGUEZ DE DIOSPhotographer -JMYuste [email protected]

DAVIDE FAGGIONATODepartment of Ecology, Evo-lution, and Organismal Biol-ogy, Iowa State [email protected]

EFTHIMIA PAPASTAVROSVeterinary Diagnostic and Pro-duction Animal Medicine,Iowa State [email protected]

EUGENIA MONTIEL-JIMENEZDepartment of Ecology, Evo-lution, and Organismal Biol-ogy, Iowa State [email protected]

HARTANTO WIBOWODepartment of Civil, Constr.,and Env. Eng., Iowa State Uni-versity [email protected]

CHRISTOPHE FRANKIEWICZDepartment of Mechanical engi-neering, Iowa State [email protected]

https://www.flickr.com/photos/jmyuste/

Directions and Lunch Menu

Campus map

The Symposium will be held in the Molecular Biology Building on Pammel Dr. on the ISU campus.Coming from Interstate 35 turn on Highway 30 West. Take exit 146 and drive north towards the Iowa State University Campus.Continue on University Blvd for approximately 2.6 miles then continue straight onto Pammel Dr. After approximately 1 mile turnright on Morrill road to reach Parking Lot #29 on the left. The Molecular Biology Building is located at Pammel Dr. on the right.

52 Directions

Molecular biology building map

53 Lunch Buffet Menu

Lunch Buffet Menu

ENTRÉE SELECTION

Classic Roast BeefRotisserie Breast of TurkeySweet and Spicy Salmon

Three-cheese Lasagna

•••

SIDE SELECTION

Oven Roasted PotatoesRice Pilaf

•••

VEGETABLE AND SALAD SELECTION

Catalina VegetablesCaesar Salad

Vegetarian and gluten-free dishes are include in the buffetFood catered by HyVee

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