naturally occurring acacia spirorbis in new caledonia: a ...€¦ · naturally occurring acacia...
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Naturally occurring Acacia spirorbis in New Caledonia: a top-notch nitrogen fixing species through extremely contrasted soils.
Bryan VINCENT
Philippe JOURAND
Farid JUILLOT
Marc DUCOUSSO
Antoine GALIANA
New Caledonia = Hotspot of biodiversity
A: Dry forests B: Ultramafic ecosystems C: Calcareous islands D: Mangrove
Pacific archipelago 1800 km East of Australia Coral reef
Rehabilitation of post-mining soils
strong polymetallic toxicity = extremes conditions
imported Eucalyptus and Australian Acacias failed to survived
focus on endemic species adapted to such environments
• phyllodenous Acacia species resulting from natural dispersal of Australia
• endemic to New Caledonia
• naturally occurring over contrasted soils
• symbioses with AMF, ECM and rhizobia = often mentioned as helpers in plant adaptation
Acacia spirorbis subsp. spirorbis Labill. (1825)
Rhizobia ECM AMF
What is the symbiotic N2-fixing potential of A. spirorbis ? Is the N2 fixation efficiency impacted by soil properties ?
Study sites
Distribution of the study sites according to their soil properties
Metalliferous soils
Siliceous soils
Calcareous soils
Confirmation of 3 contrasted soil categories hosting natural A. spirorbis populations
Assessing the symbiotic N2 fixation with the natural abundance of 15N
[15N/14N]air = 0.3663 %
14N 15N
14N
15N
14N
15N
14N Inspired from Peoples et al., 1989
SOIL
AIR
No
n-N
2-f
ixin
g sp
eci
es
N2-f
ixin
g le
gum
e
nodules
Formula of δ15N and %Ndfa
• δ15N (‰) = 1000 x %15𝑁
𝑠𝑎𝑚𝑝𝑙𝑒 − 0.3663
0.3663
– If δ15Nlegume close to 0 = similar to air = N2 fixation
– If δ15Nlegume close to δ15Nnon-fix = no N2 fixation
• %Ndfa = 100 x (δ15Nnon−fix − δ15Nlegume)
(δ15Nnon−fix − β)
PM
S P
MS
TIE
SRP
P
MS TI
E PN
2
PN
2
PIN
P
N2
P
MB
SR
P
EAS PM
S TIE
PM
B
PN
2
PIN
V
RT
EAS
PIN
W
AN
EAS
VR
T W
AN
EA
S W
AN
WA
N
PIN
EA
S W
AN
V
RT
-6,00
-4,00
-2,00
0,00
2,00
4,00
6,00
8,00
10,00δ
15N
(‰
)
A. spirorbis
D. viscosa
Melaleuca sp.
M. citrifolia
P. nummularoides
Sannantha sp.
Scaevola sp.
W. indica
Results of δ15N for A. spirorbis and the non-N2-fixing reference species
large range of δ15N for the reference species the δ15N of A. spirorbis is centered around 0 ‰ = similar to atm
Proportion of N fixed by A. spirorbis on metalliferous soils
Site Non-N2-fixing reference species Acacia spirorbis
Name δ15Nnf (‰) δ15Nf (‰) %Ndfa
PN2
Dodonaea viscosa -1.68 ± 0.55
+0.35 ± 0.97
100
Melaleuca quinquenervia -1.25 ± 1.05 100
Sannantha sp. -1.15 ± 0.51 100
PIN
Dodonaea viscosa -1.16 ± 0.72
+0.45 ± 0.79
100
Melaleuca quinquenervia +4.43 ± 0.36 74
Wikstroemia indica +1.46 ± 0.22 42
TIE Melaleuca quinquenervia -2.09 ± 0.07
+0.09 ± 0.09 100
Sannantha sp. -3.28 ± 1.37 100
Proportion of N fixed by A. spirorbis on calcareous soils
Site Non-N2-fixing reference species Acacia spirorbis
Name δ15Nnf (‰) δ15Nf (‰) %Ndfa
EAS
Morinda citrifolia +4.86 ± 1.77
-0.88 ± 0.74
99
Psychotria nummularoides +2.06 ± 0.72 98
Scaevola sp. +2.97 ± 0.59 99
Wikstroemia indica +2.94 ± 1.18 99
WAN
Morinda citrifolia +7.05 ± 1.47
+3.13 ± 0.54
49
Psychotria nummularoides +4.10 ± 0.75 19
Scaevola sp. +2.97 ± 0.14 0
Wikstroemia indica +2.28 ± 1.27 0
Proportion of N fixed by A. spirorbis on siliceous soils
Site Non-N2-fixing reference species Acacia spirorbis
Name δ15Nnf (‰) δ15Nf (‰) %Ndfa
PMB Melaleuca quinquenervia -1.09 ± 0.22 +0.21 ± 0.11 100
PMS
Melaleuca quinquenervia -2.71 ± 1.11
-0.20 ± 0.83
100
Sannantha sp. -4.81 ± 0.42 100
Wikstroemia indica -4.83 ± 0.09 100
SRP Dodonaea viscosa -3.22 ± 0.03 -1.04 ± 0.42 95
VRT Melaleuca quinquenervia +5.17 ± 0.23
+0.50 ± 0.29 76
Wikstroemia indica +2.97 ± 0.95 63
What is the N2-fixing potential of A. spirorbis ? • very high %Ndfa compared to other legumes
o Acacia mangium 50% (Ivory Coast)
o A. melanoxylon 43 % (Australia)
o A. mucronata 48 % (Australia)
o Calliandra calothyrsus 50 % (Australia)
o A. spirorbis 90 % (New Caledonia) = top-notch
Is the N2 fixation affected by contrasted soils ? • very effective on most of the sites = independent from soil N ?
• A. spirorbis = currently tested as a nurse plant in post-mining soils
Nitrogen fixation plays a key role in A. spirorbis adaptation to soils conditions
Thank you ! Question time ?
Study sites characteristics
Soil properties of the study sites
Element contents are expressed in mg.kg-1
Nitrogen contents and δ15N of the soil, reference species and A. spirorbis