FEATURES OF METABOLIC PROCESSES IN CARPFEATURES OF METABOLIC PROCESSES IN CARP((Cyprinus carpio L.Cyprinus carpio L.)) INFLUENCED BY WASTE WATER FROM INFLUENCED BY WASTE WATER FROM

LIVESTOCK ENTERPRISESLIVESTOCK ENTERPRISES

ReportsReports – – Inna N. KurbatovaInna N. Kurbatova SupervisorSupervisor – – ProfProf. . Mykola O. ZakharenkoMykola O. Zakharenko

NATIONAL UNIVERSITY OF LIFE AND ENVIRONMENTAL NATIONAL UNIVERSITY OF LIFE AND ENVIRONMENTAL SCIECES OF UKRAINESCIECES OF UKRAINE

Institute of animal science and aquatic bioresourcesInstitute of animal science and aquatic bioresources

GGoal of scientific workoal of scientific work

Examine the factors of human influence on Examine the factors of human influence on the aquatic environment the aquatic environment (organic and (organic and

inorganic origin, inorganic origin, their sources and content in their sources and content in the waterthe water, ,

physico-chemical properties of water; physico-chemical properties of water; their their effects on physiological and biochemical effects on physiological and biochemical

processes in fishprocesses in fish).).

Scientific research jobScientific research job

Explore the influence of waste water from livestock Explore the influence of waste water from livestock enterprises in development of eggs and larvae of enterprises in development of eggs and larvae of

carpcarp((Cyprinys carpio L.Cyprinys carpio L.)), , physiological and physiological and biochemical processes in the hepatopancreasbiochemical processes in the hepatopancreas

Figure 1 The general scheme of studies

Waste water from Waste water from livestock enterpriseslivestock enterprises

EggsEggs LarvaeLarvae

Organic and inorganic

xenobiotics

Laboratory fish(model experiments)

Organic and inorganic

xenobiotics

LDG, alkaline phosphatase,AlAT, AsAT in blood plasma

oxidative phosphorylation

in the hepatopancreas

Total protein, urea,

hemoglobin in blood plasma

Figure 2 Model experiments in aquarium

Figure 3 – Mass-spectrometric analysis of waste water from

liverstock enterprises

(izovalerian acid, lubricant acid, propionic acid, hexanoic acid, heptanoic acid, benzoic acid, 9-octodecanoic acid , aminoec salt of benzoic acid, phenylacetic acid, phenylpropionic acid, p-cresol, phenol, 2-acetyl-aniline, 4-methylindole, 7-methylindole3-butyl-hydroxyanisole, 3,4-digidrokarbosteril)

GroupGroup IndicatorIndicatortotal protein, ,

gg//llurea,,mmolmmol//ll

hemoglobin ( (blood),),gg//ll

ccontrolontrol 26,95±2,6326,95±2,63 0,33±0,030,33±0,03 90,50±6,4290,50±6,42

11 27,90±2,1827,90±2,18 0,38±0,02*0,38±0,02* 89,00±5,7789,00±5,77

22 32,30±3,89*32,30±3,89* 0,41±0,02*0,41±0,02* 93,00±4,79*93,00±4,79*

33 34,37±4,29*34,37±4,29* 0,44±0,03*0,44±0,03* 98,00±5,59*98,00±5,59*

44 35,95±3,77*35,95±3,77* 0,50±0,05*0,50±0,05* 105,00±7,48*105,00±7,48*

Table 1 Biochemical parameters in blood plasma of carp at different levels of waste waterwater in the aquarium, М±m, n=8

Note. * р<0,05, data probability compared with the control

Table Table 2 2 Enzyme activity in blood plasma of carpEnzyme activity in blood plasma of carp at different at different levels of waste waterlevels of waste water in the aquariumin the aquarium, М±m, , М±m, nn=8=8

GroupGroup IndicatorIndicator

LDGmkkat/l

Alkaline

phosphatasemkmol/ml/h.

AlATmkmol/mg of protein

/h

AsATmkmol/mg of protein

/h

ccontrolontrol 587,87±12,43 47,63±6,27 31,60±2,48 26,75± 1,23

1 519,45±10,11* 50,40±3,91 35,50±2,32* 29,00±0,95*

2 640,34±13,74* 40,10±4,34* 46,10±4,90* 32,40±2,11*

3 704,23±16,89* 35,70±3,67* 40,10±3,45* 36,40±2,66*

4 914,90±24,51* 30,65±1,99* 66,00±4,88* 127,40±7,21

Note. * р<0,05, data probability compared with the control

CONCLUSIONSCONCLUSIONSWhen different concentrations of waste water When different concentrations of waste water from pig farms are added to aquarium water, from pig farms are added to aquarium water, carp demonstrates hindered reaction of tissue carp demonstrates hindered reaction of tissue respiration, the processes of anaerobic respiration, the processes of anaerobic glycolysis in cells are activated, as evidenced glycolysis in cells are activated, as evidenced by the increasing LDH activity and decreasing by the increasing LDH activity and decreasing LF activity in the blood of fish compared with LF activity in the blood of fish compared with control; hypoxia occurs in organism, which is control; hypoxia occurs in organism, which is indicated by growth of hemoglobin in blood of indicated by growth of hemoglobin in blood of experimental fish; the intensity of ammonium experimental fish; the intensity of ammonium and ureohenez processes in tissues changes, and ureohenez processes in tissues changes, as evidenced by the increasing concentration as evidenced by the increasing concentration of urea and increased activity of intracellular of urea and increased activity of intracellular ALT, AST enzymes in the blood of fish ALT, AST enzymes in the blood of fish compared with control.compared with control.