nanotechnology and infectious diseases
TRANSCRIPT
NANOTECHNOLOGY IN INFECTIOUS DISEASES Major Inam Danish Khan
Dept of Microbiology and Molecular MedicineArmy Hospital Research and Referral, New Delhi
PROLOGUE
Golden ages of Microbiology Introduction History of nanotechnology Scope of nanotechnology Visualization Diagnostics Nano-engineered products Emerging nano-concepts Nanotechnology issues
GOLDEN AGES OF MICROBIOLOGY First Golden Age
Bacterial physiology and nutrition Cultivation methods Immunology, phagocytosis Antibodies Vaccines Viruses
Second Golden Age Cellular immunology Monoclonal antibodies Transposons Genetic engineering Nucleic acid sequencing Protein technology
Third Golden Age Emerging infectious diseases Identification of uncultivated microbes Role of microbes in modulating host development Role of microbes in chronic diseases Rapid identification of microbes Use of microbes as NANOMACHINES
INTRODUCTION Nanoscience encompasses the common unifying concepts and
physical laws that prevail in the nanoscale 1 nanometer = 10-9 meter (1 billionth of a meter) Greek ‘nanos’ or Latin ‘nanus’ means dwarf Physical, chemical, optical and electrical properties differ
Nanotechnology is the understanding and control of matter at dimensions in the nanoscale
Nanotechnology consists of the process of separation, consolidation and deformation of materials by one atom or one molecule
Nanotechnology is the creation of functional materials, devices and systems, through the understanding and control of matter at dimensions in the nanometer scale length (1-100 nm), where new functionalities and properties of matter are observed and harnessed for physical, chemical and biological interest
NANOTECHNOLOGY TERMS Cluster - A collection of up to 50 units (atoms or molecules) Colloid particle - 1-1000 nm particle in liquid phase Nanoparticle - 1-100 nm particle that could be noncrystalline,
aggregate of crystallites or a single crystallite 1st generation Nanoparticles: <100nm, 2nd generation Nanoparticles: <10nm
Nanocrystal - Single crystal in the nanometer range
HISTORY OF NANOTECHNOLOGY ~2000 BC: Sulfide nanocrystals used by Greeks & Romans to dye hair Photography and catalysts 1959: R. Feynman - There's Plenty of Room at the Bottom
1974: Taniguchi 1981: Scanning Tunneling microscope 1985: Buckyballs (Buckminster fullerenes) – R Kroto 1986: Atomic Force Microscope 1991: Carbon nanotube - S.Lijima
NANOTECHNOLOGY IN INFECTIOUS DISEASES
Diagnostics Monitoring Therapeutics
Surveillance Prevention Research
SCOPE OF NANOTECHNOLOGY Nanotechnology is any technology which operates in the nanoscale
Exploring the methodology of nano-operations Physics, chemistry and biology of nanotechnology
Enabling visualization and studies in the nanoscale Biomolecular interactions, pathogen interactions Equipments - EM, FIBM, DBM, SPM – STM & AFM
Designing and construction of nano-engineered products Biological nanoparticles, synthetic nanomaterials, biosensors
Detection and monitoring of deployed technology Disease surveillance Equipments - TIRM , Optical nanoscopy, SERS
Nanotechnology applications in disease diagnosis Nanoarray, quantum dots, Lab on a chip
Nanotechnology in targeted therapies/preventive interventions Pharmacy on a chip, targeted drug delivery, DNA vaccines
Research on nanoparticles
ELECTRON MICROSCOPY Use of electron beam (shorter wavelength than light) to produce images Source of illumination – Heated tungsten filament, cathode, electron gun Electron cloud propelled by high volatge 50-100 kV High vacuum of the order of 10-6 mm Hg is maintained Magnification 10000X to 100000X Copper grid 2-3 mm dia - Mesh size 200 for tissue & 400 for microbes EM lenses Limitations Sample has to be dry/dead Electrons are ionizing and
may damage the specimen Accumulated electrons in the
sample repel electrons in beam
ELECTRON MICROSCOPY TEM – 1931 – Max Knoll and Ernst Ruska – Nobel prize to Ruska in 1986 Bulk beam transmitted non scattering electrons Electrons pass through thin specimens (50-1000 nm) – OsO4/KMnO4
2D image on fluorescent screen - Denser regions appear darker Resolution – 0.005 nm (Theoretical), 0.05 nm (Practical) ----- 200 nm light
SEM – 1935 – Max Knoll Narrow beam electrons reflected from
the surface of thick metal coated specimens Signal sent to cathode ray tube Resolution – < 7 nm 3D image like a television picture Lower magnification than TEM
ELECTRON MICROSCOPY Confocal SEM – Laser beam illuminates spots on the specimen Analytical EM – Elemental composition of materials within tissues STEM – 1983 von Ardenne, resolution 0.05 nm Immunoelectron microscopy – TEM/SEM imaging of specimens labelled
with gold particles (1-40 nm) conjugated to primary Ab against target Ag Visualisation of Ag within ultrastructural images
Staphylococcus Streptococcus Strep pneumoniae
E coli Pseudomonas Vibrio cholerae
Helicobacter Capsule RBCs
Rotavirus under IEM Influenza virus Bacteriophage
Adenovirus Release of viral progeny Mitochondria
Staph epidermidis biofilm Cytopathic effects Golgi body
Aspergillus, sporangium surrounded by hyphae
Influenza Decondensation of chromatin Silicon atoms
1985 Electron beam replaced by positively charged Gallium ions Liquid metal coated Tungsten needle as ion source Cross sectional imaging possible through sputtering
FOCUSSED ION BEAM MICROSCOPY
DUAL BEAM MICROSCOPY SEM + FIB Microscopy High resolution Suitable for fragile specimens
Uses variety of different interactions of a fine tip with the specimen Image created by physical contact of probe moving across the specimen Piezoelectric elements facilitate precise movements Carbon nanotube probes held at atom’s diameter from sample Electrons tunnel between the tip and specimen, producing a signal Living objects can be examined
Scanning Tunneling Microscope 1981: Binnig and Rohrer – Noble prize 1986 Resolution - 0.1 nm lateral, 0.01 nm depth Used in ultra high vacuum, air, liquid or gas Temp ranging from near zero Kelvin to a few hundred degrees Celsius Probe moves up and down on the image with steady tunneling current Variation in current is detected to create image
SCANNING PROBE MICROSCOPY
SCANNING PROBE MICROSCOPY Atomic Force Microscope 1986: Binnig, Quate and Gerbe Resolution: 5 nm (lateral), 0.01 nm (depth), higher in air Sharp probe moves over specimen surface at constant distance Up and down movement of probe is detected to create true 3D image No specimen preparation required Cell membrane, flagella, protein, nucleic acid, secretions, DNA sequencing Tapping mode and lift mode AFM Limitations Low magnification Cannot delineate steep walls or overhang Images affected by choice of tip Piezoelectric limitations
TOTAL INTERNAL REFLECTION MICROSCOPY Designed for measurements of surface diffusion in biosensors DC power supply for electrophoretic flow characterization Receptor ligand interactions at nanoscale
OPTICAL NANOSCOPY Designed for measurements of surface diffusion in biosensors DC power supply for electrophoretic flow characterization Receptor ligand interactions at nanoscale
MICROARRAY/NANOARRAY Microarray - High-throughput analysis of biomolecules
Requires large sample volume, prolonged incubation time Bulky instrument for detection, laborious procedure
Nanoarray – Higher sensitivity, simple sample preparation Biomolecular analysis Monitoring of trace pathogens Nucleic acid, enzyme, protein detection using AFM
SURFACE ENHANCED RAMAN SPECTROSCOPY Identifies even single molecule based on its vibrational energy Rapid DNA sequencing Pathogen detection in clinical and environmental samples Nanostructure characterization
TYPES OF NANOMATERIALS Unidimensional – Surface coatings, engineered surfaces, thin films
Kaolinite derived aluminosilicate nanoparticle infusion in traditional gauze Water absorbing zeolite that concentrates coagulation factors to stop bleeding Bioceramic materials
Bioinert Nanoalumina implant Bioresorbable Hydroxyapatite/tricalcium phosphate coating on metallic orthopaedic implant Bioactive Bioglass/Apatite wollastonite glass
Nanofunctionalized zirconia and bone cement additives – Synthetic bone
Bidimensional – Carbon nanotubes, nanowires, biopolymers Tridimensional – Quantum dots, dendrimers
Plasmid DNA expression vectors in wounds to enhance growth factors Electroporation mediated transfection Starbust dendrimers get endocytosed and release DNA to nucleus Polyamidoamine (PANAM) dendrimer for in vitro gene delivery – Hypoxic injury Nanoengineering of stem cells to make organs
BIOLOGICAL NANOPARTICLES Proteins, enzymes, peptides, DNA, RNA Genetically engineered fluorescent viruses identify E. coli by infection
Fluorescent microscope detect glow in a few hours Use of bacteria to transport ‘smart nanoparticles’ to specific targets
Precise position of sensors within cells Drug/DNA delivery Diagnosis and treatment of diseases Carbon nanotubes delivered to target,
heated, selective killing of diseased cells
Polymers Gelatin, albumin Polyethylene gycol, polylactide,polyepsilon caprolactone, polyalkylcyanoacrylate
Porous silicon Carbon nanotubes Carbon nanospheres Dendrimers <15 nm
Deliver DNA in gene therapy
Nanogold Nanoscale sensors and actuators Respirators Protective clothing – Nanobionic in hypothermia
SYNTHETIC NANOMATERIALS
Nanowires – Lateral dimension 1-100 nm
SYNTHETIC NANOMATERIALS
QUANTUM DOTS Combination of microfluidics, magnetic particles and gold nanoparticles
encoded with antibodies and DNA Extremely high sensitivity Electrochemical immunoassay - IgG Detect individual biomolecules and virus particles
SYNTHETIC NANOMATERIALS: BIOSENSORS EM LF-ICT Pathogen detection Screen for disease markers (Infections, cancer)
Fluorescent organic dye attached to Salmonella Ab
Targeted/controlled drug therapy Magnetic nanoparticles Stealth nanoparticles - PEG – Least opsonization on the surface in vivo
Least uptake by macrophages – Persistence in blood Silver nanocrystals for antimicrobial wound dressing
Releases clusters of highly reactive silver cations upto 100 ppm on contact with water Causes electron transport , cell membrane damage, inactivation of bacterial DNA, binding of
insoluble complexes in microbes Nanoparticle cream for delivery of nitric oxide to treat infection Drug delivery across blood brain barrier Nanosphere liposomes, nanocapsules
SYNTHETIC NANOMATERIALS: BIOSENSORS Biomolecular interactions
Immobilization of ssDNA on cantilever Electrochemical detection of DNA hybridization DNA sequencing using nanoprobes Identification of molecular recognition Identification of self assembly motifs
MWNT biosensors for detection Hydrazine in hypertension Epinephrine and dopamine in Parkinsonism Glucose in diabetics Cholesterol, uric acid Hemin modified sensors for oxygen, NADPH, H2O2, NO Organophosphates
SYNTHETIC NANOMATERIALS: BIOSENSORS Imaging technologies – Qdot nanocrystals
Live cell imaging and dynamics - Multicolour analysis Permanent sample storage in pathology
Environmental monitoring Interferometric sensor
Binding of virus particle to Ag specific (haemagglutinin) Ab Handheld device for detection and quantification of virus Rapid screening of pathogens from samples in outbreaks
LAB ON A CHIP
EMERGING NANO-CONCEPTS Rapidly Adaptable Nanotherapeutics Inhaled nanoparticles loaded with siRNA Can target and shutdown specific genes Specific microbes can be targeted Successful in HCV Being tested against bacteria Possible use against bioweapons DNA vaccines for bacteria, viruses, parasites Silver ions + oxygen can destroy HIV Peptide nanoparticles overcome resistance
EMERGING NANO-CONCEPTS Pharmacy on a chip Nanorobots – Mobile ATP energized nanomachines
Respirocyte (1μ RBC) – 10000 times more efficient than RBC Microbivore (3.4μ X 2μ Phagocyte)
Ingested bacteria converted to aa, ffa, sugar, nucleotides
Nanomimicry Nanorubber - Self healing rubber Artificial tissues and organs Nanopistons (Incorporation of H2, O2, N2 in nanotubes) Nanomaterials can trick bacteria to sense a quorum early when there are
few bacteria. This will prompt natural immunity to overcome them Force spectroscopy to manipulate single membrane proteins
Mapping of surface properties and receptor sites Measurement of cellular interactions at single cell/ molecule level
FUTURE Artificial blood Nanorobotics - Respirocyte (1μ RBC)
10000 times more efficient than RBC
NANOTECHNOLOGY ISSUES Ethical, legal and social aspects No long-term experience Few exposure assessments Toxicity: Few toxicological assessments Removal difficult Pollution Entry into food chain Stem cell research Self replicating machines
THANK YOU