nanosensors
DESCRIPTION
Nanosensors. Hyunkyung Bae Byungmook Kim. Why is it needed?. How to make?. What to do?. Nanosensors. Big Issues in Biology. Google image. Properties of Nanosensors. 1. Magnetic Property. 2 . Optical property. Google image. Magnetic properties. Superparamagnetism. - PowerPoint PPT PresentationTRANSCRIPT
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Nanosensors
Hyunkyung BaeByungmook Kim
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Nanosensors
Why is it needed?
How to make?
What to do?
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Big Issues in Biology
Google image
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Properties of Nanosensors
Google image
1. Magnetic Property 2. Optical property
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Magnetic properties
Ferromagnetism Paramagnetism. Ferrimagnetism Antiferromagnetism
Ordered and same magnitude without external magnetic field
Disordered without external magnetic fieldIn the external mag-netic field, the mo-ments are ordered.
Aligned oppositely with different magni-tude
Aligned oppositely with the same magni-tudes
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Superparamagnetism
• Superparamagnetism is a form of magnetism, which appears in small ferromagnetic or ferromagnetic nanoparticle.
• Neel relxation time < measurement time→average zero magnetic state = superparamagnetic state
• When an external magnetic field is applied to an assembly of superparamagnetic nanoparticles, their magnetic moments tend to align along the applied field, leading to a net magnetization.
N S
Nanoparticle
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Optical properties
IGOR L. MEDINTZ1*, H. TETSUO UYEDA2, ELLEN R. GOLDMAN1 AND HEDI MATTOUSSI, Quantum dot bioconjugates for imaging, labelling and sensing, Nature materials 2005
Alexa 488
FITC
GFP Quantum dots
Organic dye
0 10 20 30 40 50 60 70 80 90100
110120
130140
1500
0.2
0.4
0.6
0.8
1
1.2
Photobleaching time
FITC Alexa Flour 488GFP QD 608
Time(sec)
Rela
tive
inte
nsity
Qd is Much Much stable!
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Fluorescence Resonance Energy Transfer
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How can we make it?
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Characterization
Gold nanoparticle – Optical Dark field
Quantum dots - TIRF
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What can we do with it?
1.Cell/Protein Imaging2.Cell/Protein Manipulation3.Nano ruler
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1. Cell/Protein Imaging
40mer
lipid
63mer
82mer
Lipid
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2. Cell/Protein Manipulation
Cell
Channel
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3. Nanoruler
• Alexander J. Mastroianni, David A. Sivak, Phillip L. Geissler, and A. Paul Alivisatos, Probing the Conformational Distributions of Subpersistence Length DNA, Biophysical Journal 2009
.
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3. Nanoruler
• Alexander J. Mastroianni, David A. Sivak, Phillip L. Geissler, and A. Paul Alivisatos, Probing the Conformational Distributions of Subpersistence Length DNA, Biophysical Journal 2009
.
Dark Field – Gold nanoparticles TIRF Microscopy – Quantum dots
Q dot
Magnetic+Au
Completely matching!
Glass
Strong magnet
TIRF Microscopy
Strong magnet
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Future work
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References• A.P.Alivisatos, Science, New Series, Vol. 271, No. 5251. (Feb. 16, 1996), pp. 933-937• Yadong Yin & A.Paul Alivisatos, NATURE04165, vol 437, 2005• J. M. Yuk, J.W. Park, A. Paul Alivisatos, High-resolution EM of Colloidal Nanocrystal growth using graphene liquid cells, Science 336, 61 (2012) • J. M. Yuk, J.W. Park, A. Paul Alivisatos, High-resolution EM of Colloidal Nanocrystal growth using graphene liquid cells, Science 336, 61 (2012) • Xiaogang Peng, L.Manna, W.Yang, J. Wickham, E.Scher, A.Kadavanich & A.P.Alivisatos, Shape control of CdSe nanocrystals, Nature,vol 404, 2 March 2000• L.Manna, D.J.Milliron, A.Meisel, E.C.Scher, A.Paul Alivisatos, Controlled growth of tetrapod-branched inorganic nanocrystals, nature materials, vol 2,June 2003• Haitao Liu and A.Paul Alivisatos, Preparation of Asymmetric Nanostructures through Site Modification of Tetrapods,Nano Lett., Vol. 4, No. 12, 2004 • Son, D. H.; Hughes, S. M.; Yin, Y. D.; Alivisatos, A. P., Cation exchange reactions-in ionic nanocrystals. Science 2004, 306, (5698), 1009-1012• Robinson, R. D.; Sadtler, B.; Demchenko, D. O.; Erdonmez, C. K.; Wang, L. W.; Alivisatos, A. P., Spontaneous superlattice formation in nanorods through partial cation exchange. Science 2007, 317, (5836), 355-358• IGOR L. MEDINTZ1*, H. TETSUO UYEDA2, ELLEN R. GOLDMAN1 AND HEDI MATTOUSSI, Quantum dot bioconjugates for imaging, • labelling and sensing, Nature materials 2005• Alexander J. Mastroianni, David A. Sivak, Phillip L. Geissler, and A. Paul Alivisatos, Probing the Conformational Distributions of Subpersistence Length DNA, Biophysical Journal 2009