Nanopore Membranes for Single-Molecule Detection and Characterization

Anna Schibel, Ryuji Kawano and Henry S. White

University of Utah

October 26, 2007

The Glass Nanopore (GNP) Membrane

Zhang et al. Anal. Chem. 2007, 79, 4778-4787.

Fabrication of the GNP Membrane

Zhang, B.; Galusha, J.; Wang, G.; Bergren, A. J.; Jones, R. M.; White, R. J.; Ervin, E. N.; Cauley, C. C.; Shiozawa, P.; White, H. S. Anal. Chem. 2007, 79, 4778-4787.

Bo Zhang

Nanodisk electrodeNanopore electrodeNanopore membrane

Stochastic Single-Molecule Detection

- hepta-sulfano--cyclodextrin (s7CD)

R = -OSO3-

Bound State

Unbound State

- -Hemolysin (HL)

HL insertionSingle Binding Events

Applied Voltage -40 mV

Gu e

t al., S

cience

20

01, 2

91, 6

36-6

40

1 M KCl10 mM Potassium phosphate bufferpH 7.43 M aHL backside50 M s7CD frontside

250 nm pore

Stochastic Single-Molecule Detection

R = NH3+

Clement Osei AkotoRainer Group

trans

cis - Reference

+ - a7CD

+

+ +

50 M a7CD

Voltage in wrt cis side50 M a7CD detection

Electrostatic trapping

Electrostatically trap molecule inside HL channel

1 si

a7CD

GNP radius - 95 nm

Nanoparticle Detection

Wang, G., Single Nanoparticle Sensing with Protein Ion Channel Embedded in Lipid Bilayer Supported By Glass Nanopore Membrane (unpublished)

Gold Nanoparticle with Tiopronin Monolayer

2nm

Characterization of Hairpin DNA Structures

9bp-GC 9bp-AT 9bp-TG

Common structure

1 sec

9 bp-GC

1 sec

9 bp-TG

1 sec

9 bp-AT

~1 minute

Curr

ent

Discrimination Of Individual DNA Hairpin Molecules

Heparin Detection

- --

--Heparin

Applications In Development

•Biomedical Analysis•DNA sequencing•Heparin analysis

•Homeland Defense•Chemical warfare agent detection

•Environmental Analysis•Ultra-trace level detection of pollutants•Nano-particle analysis