na hybridization

Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings

PowerPoint® Lecture Slide Presentation prepared by Christine L. Case

Microbiology

B.E Pruitt & Jane J. Stein

AN INTRODUCTIONEIGHTH EDITION

TORTORA • FUNKE • CASE

Chapter 10, part BClassification of Microorganisms


Numerical Identification

Figure 10.9


• Combine known antiserum + unknown bacterium

• Slide agglutination

• ELISA

• Western blot

Serology

Figure 10.10


Western Blot

Figure 10.12


Phage Typing

Figure 10.13


• Differences in electrical conductivity between species

• Fluorescence of some species

• Cells selectively stained with antibody + fluorescent dye

Flow Cytometry Uses

Figure 18.11


• DNA base composition

• Guanine + cytosine moles% (GC)

• DNA fingerprinting

• Electrophoresis of restriction enzyme digests

• rRNA sequencing

• Polymerase Chain Reaction (PCR)

Genetics

Figure 10.14


Nucleic Acid Hybridization

Figure 10.15


Nucleic Acid Hybridization: DNA probe

Figure 10.16


Nucleic Acid Hybridization: DNA chip

Figure 10.17


Dichotomous Key


Cladogram

Figure 10.18.1


Cladogram

Figure 10.18.2

na hybridization

Technology

cladogram figure

dna probe figure

dna chip figure

western blot figure

species fluorescence

species cells

electrical conductivity

dichotomous key