musabase pag 2018

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MUSABASE Guillaume Bauchet Mueller lab [email protected] Plant and Animal Genome, San Diego, 2018 Musabase: A Phenotyping and Breeding Database for Bananas

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Page 1: Musabase PAG 2018

MUSABASE

Guillaume BauchetMueller lab

[email protected]

Plant and Animal Genome, San Diego, 2018

Musabase: A Phenotyping and Breeding

Database for Bananas

Page 2: Musabase PAG 2018

https://btiscience.org/lukas-mueller/#lab-members

Mueller lab

- Bioinformatics

- Genomics

- Databases

MUSABASE

Page 3: Musabase PAG 2018

+ University of Queensland, Australia+ National Banana Breeding Program, India+ University of Malaya, Malaysia

Improvement of banana

for smallholder farmers

in the Great Lakes Region

of Africa

Project scope

Page 4: Musabase PAG 2018

Genomic Selection(Moses presentation)

But also:

-> Conventional breeding

-> Plant pathology

-> Parmer preferences

-> Germplasm Management

->Tissue culture

Project scope

Page 5: Musabase PAG 2018

Project scope

multiple data types…

- Phenotyping experiments- Participatory trials - Farmer surveys- Tissue culture- Sequencing data

…and a wealth of biological specificities!

- Various ploidy levels- Germplasm groups- Complex pedigrees- Plant and field size - Life cycle length

=> different tools and approaches!

Page 6: Musabase PAG 2018

=> Need for an “in situ” resource, a breeding information repository

The banana “digital ecosystem”

Ex situ conservation

Molecular data

Semantic data

Page 7: Musabase PAG 2018

Musabase

MUSABASE http://musabase.org/

Page 8: Musabase PAG 2018

MUSABASE

Field data collection: a demanding process…

Page 9: Musabase PAG 2018

MUSABASE

Field data collection: digitalize it!

Page 10: Musabase PAG 2018

Field data collection: digitalize it!

MUSABASE

Page 11: Musabase PAG 2018

Connecting dots between and within projects…

MUSABASE

Field

Lab

MGIS

Crop ontology

Page 12: Musabase PAG 2018

Field data collection: digitalize it!

MUSABASE

Barcoding

https://musabase.org/barcode

Page 13: Musabase PAG 2018

Field data collection: digitalize it!

MUSABASE

Ontologies: streamline management

http://submit.rtbbase.org/

Banana ontology Pipeline

Credits: EM-A Laporte, . Arnaud (Bioversity)

Page 14: Musabase PAG 2018

Field data collection: digitalize it!

MUSABASE

Ontologies: postcomposing

https://musabase.org/tools/composehttps://musabase.org/search/traits

Page 15: Musabase PAG 2018

Field data collection: digitalize it!

MUSABASE

Field geolocations

https://musabase.org/breeders/locations

Page 16: Musabase PAG 2018

Field data collection: digitalize it!

MUSABASE

Pedigree

David Lyon’s presentation

Page 17: Musabase PAG 2018

Field data collection: digitalize it!

MUSABASE

Surveys

https://odk.ona.io/

-> Need for dynamic data collection processes -Farmer surveys-Field procedure (crosses)-Lab procedure (tissue culture)

Page 18: Musabase PAG 2018

Field data collection: digitalize it!

MUSABASE

Wish list + Crossing tool

http://btract.sgn.cornell.edu/https://musabase.org/breeders/crosses/

Credits: Margaret Karanja, Trushar Shah (IITA)

Page 19: Musabase PAG 2018

MUSABASE

-> Link ex situ (MGIS) vs in situ (musabase) data

-> Link additional molecular resources (genome hub, gobii)

-> Additional tools for banana breeders

Perspectives: reach the “digital ecosystem”

https://brapi.org/

Page 20: Musabase PAG 2018

-> On site trainings

-> Data managers

MUSABASE

Perspectives: keep building partnership

Arusha Tanzania NARO Uganda IITA Uganda

BTI Cornell

-> Collaborations with Bioversity/Crop ontology colleagues + new partners

Page 21: Musabase PAG 2018

Connecting dots between and within projects…

MUSABASE

[email protected]

http://slideshare.net/solgenomicsField

Lab

MGIS

Crop ontology