multiple probe 215 liquid handler user's guidefor safe and correct use of the multiple probe...

Multiple Probe 215Liquid Handler

User's Guide

LT2536/©2002 Gilson, Inc. All rights reserved February 2002

Table of Contents

Declaration of Conformity

1 IntroductionSafety Precautions ............................................................... 1-1Description ............................................................................ 1-2Unpacking ............................................................................. 1-3

Standard Equipment .................................................... 1-4Accessories ..................................................................... 1-5

Customer Service ................................................................. 1-6Technical Specifications ..................................................... 1-7

2 InstallationLocator Plate Installation ................................................... 2-2Armlock Removal ................................................................ 2-3Z-Arm Installation ............................................................... 2-4

Installing the Z-Arm Cable Support Rod andZ-Arm Control Cable ................................................... 2-5

Probe Installation ................................................................. 2-6Syringe Installation .............................................................. 2-7Plumbing Connections ........................................................ 2-8

Inlet Tubing Installation............................................... 2-8Transfer Tubing Installation ....................................... 2-9Rinse Station and Drain Waste TubingInstallation ...................................................................... 2-11

Rack Setup ............................................................................ 2-13Final Z-Arm Adjustment ................................................... 2-14Electrical Connections ........................................................ 2-15

Rear Panel ....................................................................... 2-15Input/Output Ports ...................................................... 2-16RS-232 Port ..................................................................... 2-18Keypad Port ................................................................... 2-19GSIOC Port ..................................................................... 2-20Unit ID and Baud Rate/Mode Selection .................. 2-21Fuses ................................................................................ 2-23Power Cord Connection .............................................. 2-23

3 OperationFront Panel ............................................................................ 3-2

Start Button .................................................................... 3-2Stop Button ..................................................................... 3-2LED Display ................................................................... 3-2Power Indicator Light .................................................. 3-2

Start Up ................................................................................. 3-3Running Programs .............................................................. 3-4

Programs for Gilson Keypad Controller Users ........ 3-4Running Programs from the Keypad ........................ 3-5Programs for Windows 98/NT Users ....................... 3-7

Configuring the Liquid Handler ....................................... 3-8Pump Type ..................................................................... 3-9Syringe Options ............................................................. 3-9Rinse Site ......................................................................... 3-10Liquid Detector .............................................................. 3-10Adjust X&Y .................................................................... 3-11Z Height .......................................................................... 3-12Home Phase .................................................................... 3-13Safety Contact ............................................................... 3-13Bearing Life .................................................................... 3-13Sound and Display ....................................................... 3-14Emergency Contact ...................................................... 3-14

Priming the Liquid Handler ............................................... 3-15Pausing the Priming Cycle .......................................... 3-15Stopping the Priming Cycle ........................................ 3-15

Testing the Liquid Handler’s Contacts ............................ 3-16

4 MaintenanceHelpful Hints ........................................................................ 4-2Cleaning ................................................................................ 4-3

Cleaning the Liquid Handler ...................................... 4-3Cleaning a Syringe ........................................................ 4-3Removing a Syringe ...................................................... 4-4Cleaning a Syringe ........................................................ 4-5Reinstalling the Syringe ............................................... 4-5Cleaning the Fluid Path ............................................... 4-6

Replacing Parts .................................................................... 4-10Replacing Tubing .......................................................... 4-10Replacing a Piston Seal ................................................ 4-10Replacing a Syringe ...................................................... 4-11Replacing a Solenoid Valve ......................................... 4-13Replacing One or More Probes ................................... 4-14Replacing a Fuse ............................................................ 4-16

Checking Position Alignment ............................................ 4-17Transporting the Liquid Handler ..................................... 4-18

5 TroubleshootingError Codes ........................................................................... 5-2Mechanical ............................................................................ 5-6Electrical ................................................................................ 5-7Tubing and Syringe Pump ................................................. 5-9Liquid Level Detector (if installed) ................................... 5-12Repair and Return Policies ................................................ 5-13

Appendix A Replacement Parts and Accessories

Appendix B Racks

Appendix C Specifications MethodsVolumetric Precision and Accuracy Methods ............... C-1

50 µl Sample : 500 µl Syringe ...................................... C-2500 µl Sample : 500 µl Syringe ................................... C-3100 µl Sample : 1000 µl Syringe ................................. C-41000 µl Sample : 1000 µl Syringe ............................... C-5

Carryover Method ............................................................... C-6

Appendix D GSIOC CommandsGSIOC Commands .............................................................. D-2

GSIOC Command List ................................................. D-2

Appendix E Pipetting TechniquesMinimizing Cross-Contamination .................................... E-2Minimizing Dilution Effect ................................................ E-3

Application of Council Directives:

89/336/EEC, 73/23/EEC

Standards to which Conformity is Declared:

EN61326, EN61000-3-3, EN61000-3-2, EN61010-1

Manufacturer’s Name ........................................... Gilson, Inc.

Manufacturer’s Address ....................................... 3000 W. Beltline HighwayMiddleton, WI 53562

EC Office Address .................................................. Gilson S.A.S.19 Avenue des Entrepreneurs, B.P. 4595400 Villiers-le-Bel, France

Type of Equipment ................................................. Laboratory Equipment

Model. ....................................................................... Multiple Probe 215 Liquid Handler

Beginning with Serial Number: 259A8061

Month and Year of Manufacture: January 1998

Place: Middleton, WI (USA) Michael JacquartIssue Date: December 1997 Senior Vice President

Corporate Technology Development

I, the undersigned, hereby declare that the equipmentspecified above conforms to the above Directives and Standards.

Declaration of Conformity

1-1

1

Safety PrecautionsFor safe and correct use of the Multiple Probe 215 Liquid Handler, Gilson recommendsthat both operating and service personnel follow the instructions contained in this guidewhen installing, cleaning, and maintaining the liquid handler.

Because the probes installed on the Z-arm may contain a dangerous substance, do notinterfere in the work area of the instrument until the liquid handler has completed itsprocedures. If dangerous liquids are used, adequate protection such as proper ventilation,safety glasses, and so on, should be used.

Always switch the power to off when making adjustments to the liquid handler. Thepotential exists for bodily harm if you interfere with the work area of the liquid handlerwhile it is running.

Introduction

1-2

Introduction 1De

scrip

tion Description

The Multiple Probe 215 Liquid Handler is an XYZrobot that can automate any number of manualliquid handling procedures. Its built-in syringepump provides for accurate and precise handlingof liquids.

1-3

Introduction 1U

npacking

UnpackingThe liquid handler is delivered with all majorcomponents already assembled except for auxiliaryparts such as the Z-arm, syringes, probes, racks,tubing, and so on.

The liquid handler and its components are shippedin two containers:

• One container holds the liquid handler. Keepthis container and packing assembly in case theliquid handler must be returned to the factory.

• The other container holds the auxiliary partsand any additional accessories that you orderedfor the liquid handler.

To remove the liquid handler from its container:

1 Cut the metal strapping.

2 Lift the outer box off and away from the liquidhandler.

3 Lift the inner box off and away from the liquidhandler.

4 Lift the liquid handler off its base platform andplace it on a lab bench or cart. Because of itsweight and size, Gilson recommends that twopeople lift the liquid handler off the base ofthe packing container. To lift the liquid handler:

a) Using the two cutouts for hand holds, placea hand at the base of the packing container.

b) Grip the liquid handler under the base plate.

c) Lift the liquid handler up and out of thefoam packing material. The control cabinetside, which contains the electronics, is theheavier side.

Do not attempt to lift the liquid handlerusing the Y-arm (the horizontal arm).Always lift the liquid handler from its base.

1-4

Introduction 1U

npac

king Standard Equipment

Once the liquid handler and accessories have beenunpacked from their containers, you should havethe following:

Multiple Probe 215 Liquid Handler

Z-arm (125 or 175 mm) and control cable withretaining clip

Locator plate with one drain base (includes 4mounting screws)

Accessories which include:• Rinse drain package which includes: 8-liter

waste bottle, rinse station with fittings, and 5feet of Tygon waste tubing with quick-connectfitting

• 8 PTFE inlet tubings (650 x 3 x 2 mm) with 20µm stainless steel filter

• Cable support rod with bracket and 2 Phillips–head attachment screws

• Tubing support rod• Fuse drawers, fuses, and power cords• 10-pin terminal block• 8-pin terminal block• 10 plastic locking twist ties• 9/64" ball driver for removal of armlock• Spiral Wrap

215 Setup Utility Programs CD-ROM

Multiple Probe 215 Liquid Handler User’s Guide

1-5

Introduction 1U

npacking

Accessories

Based upon your configuration, you also orderedand received additional accessories, such as probes,syringes, transfer tubing, racks, and so on. Ifnecessary, refer to Appendix A for part numbers.

1-6

Introduction 1C

usto

mer

Ser

vice Customer Service

Gilson, Inc., and its worldwide network ofauthorized representatives provide customers withthe following assistance: sales, technicalapplications, and instrument repair.

If you need assistance, please contact your Gilsonrepresentative, or if you are in the United States,contact the Gilson Customer Service Department.

Phone: 800-445-7661 or 608-836-1551Fax: 608-831-4451Email: [email protected]: www.gilson.com

To help us serve you quickly and efficiently, pleaserefer to the Before calling us section on page 5-13.

http://www.gilson.com

mail to: [email protected]

1-7

Introduction 1Technical Specifications

Technical SpecificationsThe following information is subject to changewithout notice.

Warning! Changes or modifications to the liquidhandler not expressly approved by Gilson couldvoid the user’s authority to operate the liquidhandler.

The liquid handler has been tested and found tocomply with the limits for a Class A digital device,pursuant to Part 15 of the FCC commercialenvironment. The liquid handler generates, uses,and can radiate radio frequency energy and, if notinstalled and used in accordance with theinstructions, may cause harmful interference toradio communications. Operation of the liquidhandler in a residential area is likely to causeharmful interference; in which case, the user willbe required to correct the interference at the user’sown expense.

Shielded cables must be used with the liquidhandler to ensure compliance with the Class AFCC limits.

1-8

Introduction 1

Tech

nica

l Spe

cific

atio

ns

1-9

Introduction 1

Technical Specifications

1-10

Introduction 1

Tech

nica

l Spe

cific

atio

ns

2-1

2

This section takes you through the steps for setting up a Multiple Probe 215 Liquid Handler.

Installation

2-2

Installation 2Lo

cato

r Pla

te In

stal

latio

n Locator Plate InstallationThe locator plate serves these functions:

• Positions the racks and accessories that fit ontothe liquid handler’s bed.

• Contains liquid spills, such as those caused byoverflowing vessels.

The locator plate and its four mounting screws areshipped in a separate box with the liquid handler’saccessories. To install the locator plate onto theliquid handler’s bed:

1 Make sure the locator plate’s rinsing stationbase is at the rear of the liquid handler. (Thelocator plate will only install in this orientation.)

2 Align the four corner holes of the locator platewith the four holes on the liquid handler's bedand lower the plate onto the bed.

3 Using a Phillips screwdriver, secure the locatorplate using the four mounting screws.

2-3

Installation 2A

rmlock R

emoval

Armlock RemovalThe armlock on the liquid handler secures the Y-armduring shipment. You must remove the armlockprior to installing the Z-arm and operating theliquid handler. If the armlock is not removed, theliquid handler cannot move in the X direction. Thisresults in an error state during operation.

Before you move the liquid handler to a differentlocation, always re-install the armlock. Thissafeguards against mechanical damage.

To remove the armlock:

1 Remove the cardboard label in front of thearmlock.

2 Using the 9/64" ball driver, loosen thetensioning screw that immobilizes the Y-arm.

3 Using the 9/64" ball driver, remove the tworemaining screws that hold the armlock in place.

4 Remove the armlock and store it and the balldriver for future use.

2-4

Installation 2Z-

Arm

Inst

alla

tion Z-Arm Installation

Follow these steps to install the Z-arm:

1 Using a Phillips screwdriver, loosen themounting screw on the Z-arm mountingbracket located on the Y-arm. Turncounterclockwise to loosen.

2 Partially pull out the bracket. Do not removecompletely.

3 Place the Z-arm into the mounting bracket. Youwill need to insert one side of the Z-arm intoplace at a time.

4 Tighten the screw on the mounting bracketuntil the Z-arm is secure.

You'll adjust the Z-arm to its proper height afterrack and rinse station installation. This adjustmentis described on page 2-14.

2-5

Installation 2Installing the Z-A

rm C

able Support Rod and Z-A

rm C

ontrol Cable

Installing the Z-Arm Cable SupportRod and Z-Arm Control Cable

Follow these steps to install the cable support rodand Z-arm control cable:

1 Using the 2 Phillips screws,attach the cable support rodbracket in the holes located inthe rear of the X-arm extrusion.

2 Plug the Z-arm control cableinto the back topside ofthe control cabinet.The control cableshould be tucked intothe groove located inthe top of the X-armextrusion. Theretaining clip that isalready on the controlcable should besnapped onto the top ofthe cable support rod.Refer to the diagram below.

The control cable is correctly installed when thearm is extended to the extreme X and Ydirection and the cable has enough slack.

2-6

Installation 2Pr

obe

Inst

alla

tion Probe Installation

There are different probes available for use with theliquid handler. Depending on your application, youhave purchased the appropriate probes and probeholder/guide kit. When installing the probes referto the diagram that shows where they are installedon the liquid handler.

To install the probes:

1 Insert each probe into the top of the probeholder.

2 Pull the probe through the holder until the tipof the probe is in the probe guide.

Note: When installing only four probes for use withan 849 Multiple Injection Module, install the probesin positions 1, 3, 5, and 7.

2-7

Installation 2Syringe Installation

Syringe InstallationThe following procedure is important for correctsyringe piston alignment. Improper alignment maycause premature piston seal failure. Wheninstalling syringes, install the middle two syringesfirst and work outward.

To install each syringe refer to the diagram andinstructions:

1 Press the Start button on the front panel tohome the instrument.

2 Remove a syringe from its package.

3 Lubricate the syringe's piston with reservoirsolvent in order to reduce piston seal frictionduring installation. Push the piston into thesyringe to make installation easier.

4 Remove the fourth piston holding bolt(counting from the left) from the syringe slide.

5 Finger tighten the syringe holding fitting intothe fourth position in the solenoid valveretainer. Then pull the syringe's piston until it isaligned with the hole in the syringe slide.

6 Insert and finger tighten the piston holding bolt.Then using a screwdriver, tighten until snug.

7 To prevent pulling the syringe out of alignmentwhen tightening the syringe holding fitting, usethe bottom of the syringe retainer as a guide forthe wrench. Then using a wrench, being carefulnot to hit the other syringes, tighten until snug.

8 Refer to the above steps to install the remainingsyringes. Installing the syringes in the followingorder: 4, 5, 3, 6, 2, 7, 1, and 8 will maximizeyour work area when using the wrench.

Note: Only install syringes that will be used (i.e.four syringes for four probes). Or, disable thesyringes that will not be used by removing thepiston holding bolt.

Note: Remember the size of thesyringes for later softwareconfiguration. (You can write thesize in the margin here forreference.) See Configuring theLiquid Handler in Section 3.

2-8

Installation 2Pl

umbi

ng C

onne

ctio

ns Plumbing Connections

Inlet Tubing Installation

You received eight lengths of inlet tubing (partnumber 3645357) with your liquid handler.

1 For each piece of inlet tubing, install its 1/4"-28fitting into an "R" (reservoir) labelled holelocated on top of the solenoid valve retainer.

2 Place the filtered end of the tubing into thebottle containing your diluent or probe rinsesolution.

Note: If you need to lengthen the inlet tubing,order additional tubing (part number 49948392)and couplings (part number F1410153).

2-9

Installation 2

Transfer Tubing Installation

After installing the inlet tubing, install the transfertubing.

1 Install the tubing support rod in its brackets onthe side of the control cabinet. Insert the frontend of the rod into the hole before placing theback end of the rod in its cradle.

2 Starting with transfer tubing 1 and proceedingfrom left to right, connect one end of eachtubing into a "P" (probe) labelled hole in thesolenoid valve retainer. Finger tighten and thenturn the fitting 1/4 turn with a wrench.

Note: If you're installing 5.5 mltubing, connect the wider-headfitting to the solenoid valveretainer. The narrower-head(or headless) fitting attaches tothe probe holder.

3 Lift the back end of the tubingsupport rod from its cradle.Then wrap the transfer tubingaround the rod; wrap thetubing three or four timesaround the rod. Replace thetubing support rod.

4 Connect the other end of eachtransfer tubing to thecorresponding position in theprobe holder. Firmly tightenthis fitting.

Plumbing C

onnections

2-10

Installation 2Pl

umbi

ng C

onne

ctio

ns 5 Locate the spiral wrap and cut two 4" lengths:a) Install one piece of spiral wrap around the

transfer tubing between the solenoid valveretainer and support rod.

b) Lower the Z-arm and then use the otherpiece of spiral wrap to join the transfertubing to the control cable.

6 Gather any excess tubing into a coil to minimizethe length of tubing and secure the coil using atwist tie. Position the excess tubing in aconvenient location behind the liquid handler.

7 Move the Z-arm to the farthest location in the Xand Y directions to ensure that the Z-arm canmove freely. Reposition the spiral wrap ifnecessary, or cut and install additional lengthsif necessary.

2-11

Installation 2

Rinse Station and Drain Waste TubingInstallation

The probes are cleaned using the rinse station. Toeliminate sample carryover, a rinsing procedure inyour program should pump an excess volume ofreservoir solvent or probe washing solution throughthe probe and out into the rinse station. The smalldiameter of the rinse station ports allow the outsideof the probe to be washed along with the inside.

The rinse station's design accommodates threekinds of rinses:

• Shallow-pocket rinse - Usedfor level-sensing applicationswhere the probe is onlyimmersed in a few millimetersof the sample.

• Deep-pocket rinse - Used fornon-level-sensing applications.Allows for a deeper insertionof the probe into the rinse wellresulting in a greater area ofthe outside of the probe to berinsed.

• Flow-through rinse - Used inapplications where a rigorouswash of the probe’s exterior isrequired. A second source ofliquid is pumped to the rinsestation to perform this type of rinse.

It may be necessary to vary the types andvolumes of probe wash solutions to mostefficiently eliminate carryover of particularcompounds. Generally, the smaller the volumeof probe wash solution used, the faster yourautomated liquid handling protocol.

Plumbing C

onnections

2-12

Installation 2Pl

umbi

ng C

onne

ctio

ns Installing the rinse station

The base of one rinse station is shipped alreadysecured to the locator plate and is located at therear of the locator plate. The locator plate can holdan optional second rinse station or you can movethe rinse station base to the alternate location.

Before installing the rinse station, make sure thelocator plate has been properly installed with thepreviously-installed rinse station base located at therear of the instrument bed.

To install the rinse station onto the base, followthese steps:

1 Align the triangle on the bottom of the rinsestation with the base.

2 With the rinse station's fittings facing you,insert the rinse station into the base.

3 Press down and turn the rinse stationclockwise. The rinse station is secure when youfeel the rinse station snap into place. Wheninstalled correctly, the fittings point toward thecontrol cabinet.

4 If you will be doing shallow- or deep-pocketrinses, connect waste tubing to the barbedfitting installed on the rinse station.

If you will be doing flow-through rinses,remove the plug installed on the rinse stationand replace it with a barbed fitting. Connecttubing between the barbed fitting and theexternal liquid source.

2-13

Installation 2R

ack Setup

Rack SetupThe Multiple Probe 215 Liquid Handler is equippedto locate Code 200- and Code 500-series racks. SeeAppendix B for a list of racks available for the liquidhandler.

Place the Code 200-series or Code 500-series racksdirectly onto the locator plate:

1 Orient the rack so that the code number (forexample, 200) is facing forward.

2 Fit the rack on the locator plate so that the slotsand holes on the underside of the rack alignwith the pins on the locator plate.

Note: Although the Multiple Probe 215 LiquidHandler is equipped to locate all Code 200- andCode 500-series racks, not all vessel patterns arecompatible with a four or eight probe configuration.See Appendix B for a list of racks available for usewith multiple probes on the liquid handler.

2-14

Installation 2Fi

nal Z

-Arm

Adj

ustm

ent Final Z-Arm Adjustment

Follow these steps to adjust the Z-arm to the properheight.

1 Loosen the mounting screw on the Z-armmounting bracket until the Z-arm can slide upand down. Refer to diagram on page 2-4 forlocation of mounting bracket, if necessary.

2 Slide the Z-arm and adjust to proper height.Gilson recommends that the foot of the Z-armrests approximately 5 mm from the top of thetallest vessel in a rack or from the top of therinse station.

3 Tighten the screw on the mounting bracketuntil the Z-arm is secure.

4 Determine the installed height of the Z-armusing the graduated scale located on one side ofthe drive and write it next to this step. Thisheight is required when specifying set upparameters for the liquid handler usingSET_215.EXE or the 215 Setup Utility program,described in Section 3.

2-15

Installation 2

Electrical Connections

Rear Panel

1 Input/Output (I/O) ports

2 Gilson Serial Input/Output Channel(GSIOC) port

3 Keypad port

4 RS-232 port

5 Fuse drawer

6 Power switch

7 Power receptacle

8 Unit ID selector

9 Baud rate/mode selector


2-16

Installation 2El

ectr

ical

Con

nect

ions Input/Output Ports

You can use the input and output contacts foundon the rear panel of the liquid handler to controlperipheral devices. Refer to the diagram on page2-15 for the location of the input/output ports.

Contact inputs

The input barrier strip of the liquid handler has 8connections. All of the inputs are paired, and eachpair includes a GROUND reference ( ).

The contact input pairs are labeled A, B, C, and Don the machine; however, they are referred to as 1,2, 3, and 4 in the software.

A contact is connected if it has a short across theinput or is held low by a TTL output or other device.

Never connect voltages higher than 5 V DC to aninput. When using TTL signals, be sure to matchGROUND connections.

Contact outputs

The output barrier strip has 10 contacts.

Pins 1 and 2 supply a +24V DC output. Do not usethis output unless the receiving device can accept24V power.

Pins 3 through 10 are paired, isolated-relay contactclosures and are labeled 1, 2, 3, and 4.

Items you’ll need

To make connections, you’ll need the following:

• 2-conductor cable (22 - 30 gauge for each wire)• wire insulation stripper• small-blade screwdriver

Contact the Gilson Customer Service Departmentto purchase a 6-foot piece of suitable 2-conductorcable (part number 709910206) or a package of 5cables with identification markers (part number36078155) from Gilson.

2-17

Installation 2

Making connections

To prepare and make connections with the 2-conductor cable:

1 Cut the cable into pieces of appropriate length.

2 Strip about 0.25 cm of insulation from each endof the cable.

3 Remove the terminal block connector from theliquid handler.

4 Insert each wire into the appropriate slot on thebarrier strip.

Note: When making connections, be sure tomaintain the correct orientation of the terminalblock connector relative to the port.

Push the wire all the way in; then tighten itscorresponding pin screw.

5 Connect the barrier strip to the liquid handler.The wires will be facing left and the pin screwswill be facing you as you look at the rear of theliquid handler.

Push the barrier strip in as far as it will go. It isdesigned to fit snugly into its receptacle.

6 Connect the opposite ends of the wires to theother device(s). Be sure to match groundconnections.

7 Label each cable to identify the purpose of theconnection.


2-18

Installation 2El

ectr

ical

Con

nect

ions RS-232 Port

The RS-232 port is used to transfer informationbetween the liquid handler and a computer. For thelocation of the RS-232 port on the liquid handler,refer to the diagram on page 2-15.

Be sure your computer is turned off before makingany connections.

To connect your computer to the liquid handler,you’ll need an RS-232 cable. Obtain a cable withD-connectors that are appropriate for the liquidhandler and your computer. The liquid handlerrequires a 25-pin male D-connector. Refer to theback panel of your computer or its documentationto determine which type of D-connector it requires.RS-232 cables are available from Gilson and yourlocal computer store.

Connecting the RS-232 cable

Attach the male end of the RS-232 cable to the RS-232port located on back panel of the liquid handler.Tighten the retaining screws.

Attach the other end of the RS-232 cable to thecomputer’s RS-232 serial communications port. (Donot mistake it for the female 25-pin parallel printerport!) Again, tighten the retaining screws.

2-19

Installation 2

Keypad Port

The keypad port is used to connect the GilsonKeypad Controller to the liquid handler. For thelocation of the Keypad port, refer to the diagramon page 2-15.

Using the keypad, you can run programs thatcontrol the liquid handler. See Section 3 for moreinformation on running programs using thekeypad.


2-20

Installation 2El

ectr

ical

Con

nect

ions GSIOC Port

Gilson systems feature a two-way communicationinterface between the computer and most Gilsonmodules. Communication occurs along the GilsonSerial Input/Output Channel (GSIOC).

The liquid handler can convert the RS-232 signallevels used by computers to the RS-422/485 signallevels required by the GSIOC and vice versa. (Seepage 2-18 for information on making the RS-232connection between the liquid handler andcomputer.)

GSIOC cable

Use the GSIOC cable to link an additional GilsonGSIOC module to the liquid handler and controlboth devices via a program executed on thecomputer or Gilson Keypad Controller.

Connect the female connector, located individuallyat one end of the cable, into the GSIOC port of theliquid handler. Tighten the retaining screws.

Connect the other female connector, located on thesame end as the male connector, to the Gilsonmodule. Tighten the retaining screws.

If you’re connecting another Gilson module, use themale connector to join another GSIOC cable andmake the necessary connection to the next Gilsonmodule.

2-21

Installation 2

Unit ID and Baud Rate/ModeSelection

Use the SW1 selector to choose a different unit IDand the SW2 selector to choose a different baudrate/mode. If necessary, refer to the diagram onpage 2-15 for the location of these selectors.

Unit ID

The unit ID identifies the liquid handler to Gilsonsoftware packages that can issue GSIOCcommands to the liquid handler.

At the factory, Gilson set the unit ID to 22. There isno need to change this number unless it is the sameas that assigned to another Gilson device that isalso connected along the GSIOC.

To change the unit ID:

1 Gently insert a small flat blade screwdriver intothe SW1 selector on the rear panel and turn it.

2 Align the white dot with one of the indicatednumbers. The unit ID is 20 plus the selectednumber.


2-22

Installation 2El

ectr

ical

Con

nect

ions Baud rate/mode

As a default, the baud rate/mode is set to 6,indicating that the liquid handler is set for a baudrate of 19200 and is a master device.

You’ll need to change the selection to 0 (zero) if theliquid handler is connected via the GSIOC to aGilson system and is being controlled by Gilsoncontrol software. A setting of 0 indicates the liquidhandler is a slave device and the baud rate is beingclocked externally.

Note that other selections are available if the liquidhandler is being controlled by non-Gilsonapplications. Refer to the table shown on the liquidhandler’s rear panel; see page 2-15.

To change the baud rate/mode:

1 Gently insert a small flat blade screwdriver intothe SW2 selector on the rear panel and turn it.

2 Align the white dot with one of the indicatednumbers.

2-23

Installation 2

Fuses

You may have received the liquid handler withoutany fuses installed.

1 Locate the accessory package containing thefuse drawer appropriate for your line voltage.Discard the other fuse drawer.

2 Locate the accessory package containing the2.5A “T” Slo-Blo fuse (5 x 20 mm size) fuses.

3 Install the fuse(s) into the fuse drawer. The fusedrawer for 100/120V accepts one fuse. The fusedrawer for 220/240V accepts two fuses.

4 Insert the fuse drawer into its receptacle in theliquid handler. See rear panel diagram on page2-15.

Power Cord Connection

Locate the appropriate power cord for your linevoltage. Discard the other power cord.

Use the power cord to connect the liquid handler toan AC power source.


3-1

3

The Multiple Probe 215 Liquid Handler is controlled as follows:

• Via a method running on a personal computer. You create the method usingUniPoint™ System Software, 735 Sampler Software, or 709 Sampler ManagerSoftware. All are ordered separately. See Appendix A, Replacement Parts andAccessories for part numbers.

• Via an executable file running on a Gilson Keypad Controller. You create theexecutable file using the Gilson 709 Sampler Manager Software (ordered separately,part number 2106243.)

If you have not already done so, install the UniPoint™ System Software, 735 SamplerSoftware, or 709 Sampler Manager Software. Refer to the User’s Guide included with thesoftware.

Note: When setting up a tray file for a multiple probe liquid handler, assign a zone andtube number to the first tube in each row of eight tubes to be accessed. In other words,you assign a zone and tube number to each location accessed by the left-most probe.

Operation

3-2

Operation 3Fr

ont P

anel Front Panel

The front panel of the liquid handler contains aStart button, Stop button, LED display, and powerindicator light.

Start Button

The Start button can be used to home the XYZ-armwhen the liquid handler is first powered up orwhen the motors for the XYZ-arm have beenrelaxed. When pressed, the yellow LED lights.

Stop Button

The Stop button is a large touch-sensitive pad thatcan be used to terminate a program and stop theliquid handler from responding to any morecommands coming from the running program. Thisbutton also relaxes the motors for the XYZ-arm sothat you can easily lift the probe and move the arm.When pressed, the yellow LED light is turned off.

In a situation where an emergency stop is required,pressing the Stop button immediately stops theliquid handler. The Stop button is so sensitive thatif you just brush it with your hand it activates.

LED Display

The 8-character LED display shows the currentstatus of the liquid handler and any error codes asthey are encountered. Your program can alsocontain instructions for showing 8-charactermessages on the display when the program is run.

Refer to Section 5, Troubleshooting for a list ofcurrent error codes and suggested solutions.

Power Indicator Light

This indicator becomes lit when you turn on powerto the liquid handler using the power switchlocated on the rear panel. Refer to the rear paneldiagram on page 2-15 if necessary.

3-3

Operation 3Start U

p

Start UpTo start the liquid handler:

1 Make sure the liquid handler is connected to apower source.

2 Turn on the liquid handler using the powerswitch located on the rear panel. (Refer to rearpanel diagram on page 2-15 if necessary.) Thepower indicator light on the front panelbecomes lit.

When power is turned on, the liquid handlerbeeps and displays the current version of itsinstalled firmware. This message appears forabout 1 second before the LED display returnsto a blank state.

In order to determine what PROM version isinstalled in your liquid handler, you may needto turn the unit off then on again and watchthe display for the version number to appear.

3 After the liquid handler powers up, press theStart button. This initiates the homing sequencethat allows the liquid handler to determine itsmechanical reference positions. The sequencetakes approximately 1 minute to complete.

While the homing sequence progresses, the LEDdisplay shows Homing. When the sequencecompletes, the LED display returns to a blankstate.

Note: If the program being executed by theliquid handler doesn’t include commands forhoming the instrument, perform step 3 beforestarting the program.

The utility and example programs, suppliedwith the liquid handler, home the instrument ifnecessary.

3-4

Operation 3R

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ams Running Programs

The liquid handler is controlled by programsexecuted from a Gilson Keypad Controller orpersonal computer with Windows 98 or NTinstalled.

Programs for Gilson KeypadController Users

The following programs are copied to yourcomputer’s disk as part of the 709 SamplerManager installation procedure and can be used ifyou are controlling the liquid handler via the GilsonKeypad Controller.

Program Description

SET_215.EXE Specifies configurationparameters for the liquidhandler. See page 3-8.

PRIMER.EXE Primes the transfer tubingbetween the probes andsyringes. See page 3-15.

CONTACT.EXE Enables you to test contactconnections. See page 3-16.

The Pascal versions of these programs are alsocopied to the computer as part of the software’sinstallation. You can modify them as required usingthe software.

3-5

Operation 3R

unning Programs

Running Programs from the Keypad

A Gilson Keypad Controller can be used as analternate interface between the user and liquidhandler. The keypad is connected to the liquidhandler via a coiled cable. Refer to Section 2 forcorrect installation of the cable to the Keypad port,located on the liquid handler’s rear panel.

The keypad consists of:

• an 8-line, 40-character display (whitecharacters on a blue background)

• numeric keypad with an ENTER key• four cursor direction keys• three function keys, HELP, ESC, CLEAR

• 3 1/2" disk drive with protective cover

Supplied with the 709 Sampler Manager Softwareinstallation disks is the 215 Keypad disk thatcontains the programs listed on page 3-4 and thefiles listed below. In order to run an executable filefrom the keypad, the disk on which the program isstored must contain the following files.

• MGSIOC.SYS• AUTOEXEC.BAT• CONFIG.SYS• MENU.EXE

An optional program to set the time and date isalso provided on the disk but is not required to runprograms controlling the liquid handler. Files forthis optional program are DAYTIME.EXE andDAYTIME.TXT.

To copy your liquid handler program(s) onto thekeypad disk use the Windows Explorer in MicrosoftWindows.

3-6

Operation 3R

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select one of up to 14 executable files stored on thedisk. If you have more than 14 files and you wantto maintain your keypad disk, move the utilityprograms and example programs to another disk orto a computer for storage.

To run an executable file, enter the number of theprogram corresponding to the menu selection andpress ENTER. To stop a program, press the Stopbutton on the liquid handler’s front panel or followthe program-specific instructions on stopping theprogram.

If a program prompts you for a yes or no (Y/N)response, press 1 on the keypad to indicate yes or 0to indicate no.

3-7

Operation 3R

unning Programs

Programs for Windows 98/NT Users

Executable (.EXE) programs can be run from acomputer. The computer is connected to the liquidhandler via an RS-232 cable. Refer to Section 2 forcorrect installation of the RS-232 cable between theliquid handler and the computer.

The following utility programs are supplied on the215 Setup Utility Programs CD-ROM supplied withyour liquid handler.

215 Setup Utility - Specifies configurationparameters for the liquid handler.

215 Priming Utility - Primes the transfer tubing.

215 Contact Test Utility - Enables you to testcontact connections.

Pascal versions for the above programs areavailable upon request.

3-8

Operation 3C

onfig

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Han

dler Configuring the Liquid Handler

The liquid handler comes from the factory with itsconfiguration set by Gilson. Configurationinformation is stored in the non-volatile memory ofthe liquid handler. Prior to using the liquid handlerfor the first time, it is important to review andadjust the default configuration to make sure it iscorrect for your application.

The following pages describe how to useSET_215.EXE to configure the liquid handler. Ifyou're using the 215 Setup Utility Program, refer toits online help if you need assistance.

The SET_215.EXE file is a utility program thatallows you to review and, if necessary, change theconfiguration options set for the liquid handler.This file is supplied on a separate disk for use in aGilson Keypad Controller.

When you execute SET_215.EXE from thecomputer or keypad, a menu appears.

1 pump type2 syringe options3 rinse site4 liquid detector5 adjust X & Y6 Z height7 home phase8 safety contact9 bearing life10 sound & display11 emergency contact

To select a menu item, type its number and pressENTER. From within each menu item, typing 0 andpressing ENTER will return you to the menu.

Following is a description of each of the menu items.

3-9

Operation 3C

onfiguring the Liquid Handler

Pump Type

Use this menu item to indicate that a Gilson 8Valve Syringe Pump is installed in the liquidhandler. If the pump type actually installed doesnot match the setting indicated in the configurationfile, an error will occur during operation.

Pump type selections are:

• Gilson m402 Module• 8 Valve Syringe Pump• Blank front (no syringe pump installed)

Factory default indicates that an 8 Valve Gilson 402Syringe Pump is installed.

Syringe Options

Change the syringe size - In order to properlyaspirate and dispense requested volumes, the liquidhandler must know the size of the currentlyinstalled syringes. Incorrect settings can causeimproper volumes to be aspirated or dispensedduring operation or can cause an error to occur.

Factory default is 0 for the syringe size.

Input the correct syringe size in microliters. If youdon’t know the size of the syringes installed, checkthe containers in which the syringes were shippedfor size information.

Lower piston to change the syringes - Selectingoption 2 facilitates installation of a new syringe bycausing the syringe pistons to move to the bottomof their stroke. At this time, you are prompted toinstall a new syringe. Once installed, press any keyto home the syringes.

3-10

Operation 3C

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dler Rinse Site

The default rinse site is the left-most position. Theliquid handler stores this information in memory. Itallows the liquid handler to move to this locationbefore homing the syringe and prevents the spillingof waste liquid or rinse solvent.

Coordinates for the rinse station location are providedfor the X-, Y-, and Z-axis in millimeters. Refer to thefollowing table to select the correct X-, Y-, and Z-coordinates depending on the location of the rinsestation and the kind of probe rinse to be performed.You'll notice that the coordinates for a deep-pocketrinse and a flow-through rinse are the same becausethe same ports are used to perform each of theserinse types. See rinse station diagram on page 2-11.

Liquid Detector

Use this option to adjust the sensitivity of liquiddetection as desired. For the Multiple Probe 215Liquid Handler, this option is available for the left-most probe only. Liquid Level Detection cables canbe ordered from Gilson (part number 25064040).

Raising the percentage suppresses false liquiddetection while lowering the percentage increasessensitivity for situations where liquid is harder todetect. By lowering the probes so the left-mostprobe touches the liquid for one of your samples,you can manually test the current sensitivity of theliquid detection. In this manner, you can observethe amount of change you might expect with eachsample.

The factory default setting is 6%.

3-11

Operation 3C


Adjust X&Y

This option allows you to test whether the liquidhandler is properly adjusted and to make minoradjustments to the X- and Y-axis offsets if needed.Items available in this option are described below.

Items 3 or 4 within this menu should be run first tocheck the initial X and Y coordinates. Then you canmodify the X and Y offsets using items 1 and 2,respectively.

1 Alters the X offset in millimeters.

2 Alters the Y offset in millimeters.

If you changed the X or Y offset, the liquidhandler will store the new coordinates.

3 Homes the liquid handler if it is not alreadyhomed and moves the XYZ-arm to the installedrinse site. The Z-arm becomes relaxed so thatyou can manually move the probe to visuallycheck the alignment with the rinse station.Pressing any key re-homes the liquid handler.

4 Prompts you for a target location in the X-axisand then a target location in the Y-axis. TheXYZ-arm moves to this target location and theZ-arm is relaxed so that you can manually movethe probe for visual alignment confirmation on atarget site. Pressing any key re-homes the liquidhandler.

3-12

Operation 3C

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dler Z Height

The Z-arm can be clamped at an adjustable heightover the locator plate. You can set this height sothat the liquid handler is able to properly findheights that you specify. Additionally, you can setthe size of the Z-arm that is currently installed tocontrol the amount of travel in the Z-axis. If Zheight is set here to match the actual Z mountingbracket clamp, a height of 0 mm means the Z-armfoot is flat on the locator plate.

The current distance from the bottom of the Z-armto the bottom of the locator plate pins is displayedalong with the maximum height the Z-arm cantravel. Items available in this option are:

1 Sets the height of the Z tower. Use the scaleon the Z-arm to determine the height, inmillimeters, that the tower is clamped. Referto the following diagram for setting theproper height of the Z-arm.

2 Sets the amount of Z-axis travel, in millimeters,dependent on the type of Z-arm installed in yourliquid handler. For a multiple probe liquidhandler, enter a 125 mm or 175 mm drive setting.

3-13

Operation 3C


Home Phase

Use this option to display the current X and Yphase of the liquid handler. To re-phase thisinstrument, the liquid handler must home itself 10times. Press N (or 0 if using a keypad) to exit thisoption or press Y (or 1 on the keypad) to start thehoming process. Once the process is completed,you can save the new phases to liquid handler'smemory or exit without saving the new phases.

The liquid handler finds out where home is locatedby "feeling" for the back and left walls of the unit. Itexpects to find these walls in the same place eachtime. If it does not, you will get an error. If thiserror was caused by an obstruction, just clear theobstruction and try again. If the problem does notclear or if a change is made to the mechanics, youwill probably need to repeat this option to find thetrue home location.

Safety Contact

The liquid handler has provisions for connectingsafety devices that your application may require, aslong as they present a contact closure or TTL typeinterface. This option allows you to specify whichinput contact is connected to the safety device andwhat is the active state of that device. The functionof the safety contact is equivalent to pressing theStop button on the liquid handler’s front panel.

To use this option, choose one of the contacts. Oncea contact is chosen, select:

1 Activate when the contact is open2 Activate when the contact is closed0 Do not activate (default setting)

Bearing Life

This option is a visual record of XYZ travel inkilometers and the number of full strokes thesyringe pump has performed. This is a read onlyoption.

3-14

Operation 3C

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dler Sound and Display

Use this option to adjust the brightness of thedisplay, sound level and tone. Select:

1 Alters the sound level. You can choose fromthree levels of sound: 0 = quiet, 1 = middle and2 = loud (default setting).

2 Alters the tone. You can choose from two typesof tone: 0 = sine wave tone (default setting) and1 = triangle wave tone.

3 Alters the brightness of the LED display on thefront panel. You can select a range of 0 through7 where 0 is the dimmest and 7 is the brightest.Default setting is 5.

Emergency Contact

The emergency stop option provides for sending asignal to a peripheral device whenever the liquidhandler’s Stop button is pressed or safety input isactivated.

To use this option, choose one of the contacts (1 - 3).Once a contact is chosen, select:

1 Activate when the contact is open2 Activate when the contact is closed0 Do not activate (N.A.)

Note: Once the emergency output has beenactivated, reset the contact to its non-emergencystate using the 215 Contact Test Utility program orCONTACT.EXE, described on page 3-16.

3-15

Operation 3Prim

ing the Liquid Handler

Priming the Liquid HandlerFollowing is a description of how to use thePRIMER.EXE utility program to prime the syringesand transfer tubing lines with liquid. If you're usingthe 215 Priming Utility program, refer to its onlinehelp if you need assistance.

PRIMER.EXE is copied to the computer as part ofthe 709 Sampler Manager Software installation andis also supplied on a separate disk for use in aGilson Keypad Controller.

Gilson recommends using PRIMER.EXE beforeusing the liquid handler for the first time or if theinstrument has not been used for some time. Thisprogram will eliminate any air that remains in yourliquid lines.

When executed, PRIMER.EXE causes the liquidhandler to home itself. Homing will appear on theLED display. Next, you are prompted to enter apriming speed (in milliliters per minute). Once aspeed is entered, priming begins immediately and amessage is sent to the screen confirming the size ofthe current syringe. At the same time, Priming willappear on the LED display on the front panel.

Pausing the Priming Cycle

You can pause the priming cycle at any time bypressing any key. If a pause is activated while thesyringe pump is in a downward motion, eachsyringe is stopped at the bottom of its stroke. If apause is activated while the syringe pump is in anupward motion, each syringe is stopped at the topof its stroke.

Pausing the priming cycle also causes Pause todisplay on the LED display on the front panel. Toresume the priming cycle, press 1.

Stopping the Priming Cycle

To terminate priming, press any key then press 0.This causes the syringe pump to home and Done toappear on the LED display.

3-16

Operation 3Te

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cts Testing the Liquid Handler’s

ContactsFollowing is a description of how to useCONTACT.EXE to activate output contacts todetermine if the correct contact connections havebeen made to peripheral devices to be controlledby the liquid handler. It also identifies the state ofinput contacts. If you're using the 215 Contact TestUtility, refer to its online help if you needassistance.

CONTACT.EXE is copied to the computer as partof the Gilson 709 Sampler Manager Softwareinstallation and is also supplied on a separate diskfor use in a Gilson Keypad Controller.

Before running this program, you need to connectthe peripheral device’s inputs to the appropriateoutput pair on the liquid handler. If necessary,refer to Section 2 for information on makingcontact connections.

Once CONTACT.EXE is running, the programcontinuously displays the state of outputs andinputs. You can toggle the state of any of the fiveavailable output contacts. For example, if you wantto test that output contact 1 is triggered, type 1. Ifyou want to toggle the +24 V output, type 5.

Caution: If you are running the CONTACT.EXEprogram from the keypad and toggle the +24Voutput, you’ll lose power to the keypad. To preventthis, make a jumper connection between pins 1 and2 (output 5) and the pin pair for one of the otherfour outputs. Instead of toggling output 5, togglethe appropriate output for the pin pair connectedto output 5.

4-1

4

To obtain optimum performance and maximum life from the Multiple Probe 215 LiquidHandler, it is important to keep the liquid handler well-maintained.

This section contains some general guidelines that will help you to maintain the liquidhandler.

Maintenance

4-2

Maintenance 4H

elpf

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ints Helpful Hints

In order to keep your liquid handler at peakperformance, Gilson recommends that you do thefollowing:

• Change or clean the piston seals and tubingregularly to maintain maximum syringe pumpperformance.

• Do not cycle the syringe pump without fluid.Doing this causes excessive piston seal wear.

• Flush the syringe pump, probes, and rinsestation(s) daily with distilled or deionizedwater. On a weekly basis, flush theseaccessories with a 10% solution of bleach orweak detergent.

• If bubbles remain in the syringes after priming,clean the syringes with alcohol.

• Check periodically to ensure that all fittings aretight.

• Check that each syringe is tightly connected tothe solenoid valve retainer and multiple probesyringe slide. Refer to diagram on next page.

• Wipe up all spills immediately.

• Cold fluids may cause leakage; warm fluids toroom temperature before running them throughthe system.

4-3

Maintenance 4C

leaning

Cleaning

Cleaning the Liquid Handler

The liquid handler should be cleaned occasionallyusing a dry, clean cloth. Or, if necessary, use a clothdipped in soapy water. If liquid is accidentallyspilled on the liquid handler, wipe the instrumentusing a dry, clean cloth.

Cleaning a Syringe

Clean a syringe when some or all of the followingoccurs:

• Corrosive or hazardous liquids have beenpumped

• Possible back flow of liquids into the wastetubing

• Leakage• Aspiration of samples or reagents into a syringe

To clean a syringe, follow the procedures on the nextpage and use the diagram below as a reference.

4-4

Maintenance 4C

lean

ing Removing a Syringe

Before cleaning a syringe, disconnect it from thesolenoid valve retainer and multiple probe syringeslide as described below. The following proceduresuse the SET_215.EXE program; however, you canuse the 215 Setup Utility program instead.

1 Start the SET_215.EXE program, described inSection 3.

2 Select Syringe Options from the SET_215.EXEmenu. The installed syringe size is displayedalong with an options menu.

3 Select the Lower piston to change the syringeoption. This causes the piston to descend as thesyringe pump aspirates from the reservoir. Thesyringe pump will stop at the bottom of thestroke, switching the valve to the outletposition.

4 When the prompt Install new syringe nowprompt appears, remove the syringe's pistonholding bolt from the syringe slide.

5 Unscrew the syringe from the solenoid valveretainer. You may need to use a wrench orneedle-nosed pliers to accomplish this step.

4-5

Maintenance 4C

leaning

Cleaning a Syringe

Once a syringe has been removed, clean it by doingthe following:

1 Place the syringe in a beaker containingmethanol. Then aspirate and dispense severalvolumes of methanol through the syringe.

2 Place the syringe in a beaker containing distilledor deionized water. Then aspirate and dispenseseveral volumes of water through the syringe.

3 Hold the syringe in one hand. Clean the syringeusing a non-abrasive cloth dampened withalcohol. Remove the piston and clean the pistonwith a non-abrasive cloth dampened withalcohol.

4 Dry the syringe and piston using a clean, lint-free cloth.

Reinstalling the Syringe

When the syringe is clean, reinstall it by doing thefollowing:

1 Lubricate the piston with reservoir solvent inorder to reduce friction on the piston sealsduring reinstallation.

2 Finger tighten the syringe holding fitting intothe solenoid valve retainer. Then using awrench, tighten the screw 1/8 turn.

3 Pull the syringe's piston until it is aligned withthe hole in the syringe slide.

4 Insert and finger tighten the piston holding bolt.Then using a flathead screwdriver, tighten thescrew 1/4 turn.

5 Press any key to home the syringe pump andreturn to the menu listing for the SET_215.EXEprogram.

4-6

Maintenance 4C

lean

ing Cleaning the Fluid Path

Depending upon your use of the liquid handler, itmay be necessary to flush the entire fluid path. Thefollowing procedures use the PRIMER.EXEprogram; however, you can use the 215 PrimingUtility program instead.

Gilson recommends that you clean the fluid path ifyou won’t be using the liquid handler for awhile orif you’re using a solution with a high saltconcentration for a probe wash or as a reservoirsolvent.

1 If necessary, place the syringe pump’s inlettubing into a beaker containing distilled ordeionized water. Check the beaker during thepriming sequence to ensure it always has liquidin it.

2 Start the PRIMER.EXE program.

3 Inspect the syringe pump for leaks.

4 Wipe up all spills on and around the syringepump immediately.

4-7

Maintenance 4

Cleaning methods

Depending on the samples or reagents that comeinto contact with the fluid path, you may need tovary your cleaning methods accordingly. Use thefollowing cleaning protocols as references andmake any changes to them as required for thesamples and reagents being pumped for yourapplication.

Proteins and peptides - Follow this procedure if thefluid path is in contact with proteins and peptides:

1 Place the syringe pump’s inlet tubing into abeaker containing a weak detergent solution.

2 Start PRIMER.EXE and pump until the entirefluid path has come into contact with detergent.

3 When the syringe pump’s syringe pistons arebeing lowered, press any key to pause thepriming sequence. The syringes will be stoppedat the bottom of their stroke, and the messagePaused will appear on the computer or keypad.

4 After 30 minutes, remove the inlet tubing fromthe detergent solution and immerse it in a bottlecontaining distilled or deionized water.

5 Press 1 to continue the priming sequence inPRIMER.EXE and pump the remainingdetergent from the syringe and tubing into awaste container.

6 Prime the fluid path a minimum of 10 cycleswith distilled or deionized water.

7 When you’re satisfied that the entire fluid pathhas been flushed with water, press any keythen 0 to end the priming sequence.

Cleaning

4-8

Maintenance 4

Acidic and basic compounds - Follow thisprocedure if the fluid path is in contact with acidicand basic compounds:

1 Place the syringe pump’s inlet tubing into abeaker containing 0.1 N NaOH.

2 Start PRIMER.EXE and pump until the entire fluidpath has come into contact with 0.1 N NaOH.


4 After 10 minutes, remove the inlet tubing fromthe 0.1 N NaOH and immerse it in a beakercontaining distilled or deionized water.

5 Press 1 to continue the priming sequence inPRIMER.EXE. Continue to prime until the fluidpath has been flushed with water.

6 When the syringe pump’s syringe pistons arebeing lowered, press any key to pause thepriming sequence.

7 Place the syringe pump’s inlet tubing into abeaker containing 0.1 N HCl.

8 Press 1 to continue the priming sequence.Continue to prime until the fluid path has beenflushed with 0.1 N HCl.

9 When the syringe pump’s syringe pistons arebeing lowered, press any key to pause thepriming sequence.

10 After 10 minutes, remove the inlet tubing fromthe 0.1 N HCl and immerse it in a beakercontaining distilled or deionized water.

11 Press 1 to continue the priming sequence.Continue to prime until the fluid path has beenflushed with water.

12 When you’re satisfied that the entire fluid pathhas been flushed with water, press any key then0 to end the priming sequence.

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4-9

Maintenance 4

Biological fluids - Follow this procedure if thefluid path is in contact with biological fluids suchas blood products:

1 Make a solution of 10% bleach by adding onepart commercial bleach to nine parts of water.

2 Place the syringe pump’s inlet tubing into abeaker containing the bleach solution.

3 Start PRIMER.EXE and pump until the entirefluid path has come into contact with bleach.


5 After 30 minutes, remove the inlet tubing fromthe bleach solution and immerse it in a beakercontaining distilled or deionized water.

6 Press 1 to continue the priming sequence andpump the remaining bleach solution from thesyringe and tubing into a waste container.

7 Prime the fluid path a minimum of 10 cycleswith distilled or deionized water.

8 When you’re satisfied that the entire fluid pathhas been flushed with water, press any keythen 0 to end the priming sequence.

Cleaning

4-10

Maintenance 4

Replacing Parts

Replacing Tubing

It is important to keep all tubing clean and free ofcrimps. Tubing that has become dirty, blocked orcrimped can result in poor accuracy and precision,loss of air gap or the syringe stalling.

Replace both the transfer tubing and inlet tubing asneeded. See Appendix A for part numbers forreplacement tubing. For tubing installationprocedures, see Section 2.

Replacing a Piston Seal

To change a piston seal, refer to the instructionssupplied with the replacement seal. For partnumbers for replacement seals, contact GilsonCustomer Service at 800-445-7661 or 608-836-1551.You can also contact the Customer ServiceDepartment via its electronic mail (e-mail) address:[email protected].

Outside the United States, contact your Gilsonrepresentative.

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mail to: [email protected]

4-11

Maintenance 4

Replacing a Syringe

If necessary, refer to the diagram on page 4-3 whileperforming the procedures below. The followingprocedures use the SET_215.EXE program; however,you can use the 215 Setup Utility program instead.

Removing the syringe

1 Run the SET_215.EXE program. Refer toSection 3 for more information on runningprograms.

2 Select Syringe options from the program’smenu. The installed syringe size is displayedalong with an options menu.

3 Select the Lower piston to change the syringeoption. This causes the XYZ-arm to move to therinse station and the syringe pump’s pistonoperating rod to descend as the syringe pumpaspirates from the reservoir. The syringe pumpwill stop at the bottom of its down stroke andthe valve will switch to the outlet position.

Note: If the liquid handler is not homed whenthis option is selected, the unit will home beforeit moves to the rinse site.

4 When the program prompt Install new syringenow appears, remove the syringe's pistonholding bolt from the syringe slide.

5 Unscrew the syringe from the solenoid valveretainer. You may need to use a wrench orneedle-nose pliers to accomplish this step.

Replacing Parts

4-12

Maintenance 4

Mounting new syringe

1 Lubricate the piston with reservoir solvent inorder to reduce friction on the piston sealsduring reinstallation.

2 Before inserting the syringe holding fitting,clean its connector in the solenoid valveretainer. Use a methanol-dampened cottonswab to clean the connector.

3 Finger tighten the syringe holding fitting intothe solenoid valve retainer. Then using awrench, tighten the screw 1/8 turn.

4 Pull the syringe's piston until it is aligned withthe hole in the syringe slide.

5 Insert and finger tighten the piston holding bolt.Then using a flathead screwdriver, tighten thescrew 1/4 turn.

6 Press any key when finished. The liquid handlerwill move to the rinse site and the syringe pumpwill re-initialize with the new syringe.

Note: If you are installing new syringes andthey are a different size than the ones beingreplaced, run SET_215.EXE or 215SETUP.EXEand change the syringe size.

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4-13

Maintenance 4

Replacing a Solenoid Valve

To replace a nonworking solenoid valve, follow thesesteps:

1 Remove the valve’s syringe, as described on page 4-4.

2 From the top of the solenoid valve retainer, detachthe reservoir tubing for the nonworking valve.

3 From the top of the solenoid valve retainer, detachthe transfer tubing for the nonworking valve.

4 Using a 9/64” hex wrench, loosen the screws thatsecure the cover of the solenoid valve retainer.

5 Looking down into the solenoid valve retainer,locate the nonworking valve.

6 Locate the cable and connector for the nonworkingvalve. Then disconnect the connector from theelectronics board. Note the orientation and locationof the connector so it can later be reinstalled to theappropriate location.

7 Remove the connector located above or below theconnector that you disconnected in step 6. Onceagain, note the orientation and location of theconnector so it can later be reinstalled to theappropriate location.

8 Remove the nonworking valve by grasping the valveand gently pulling it about 3/8” towards you. Liftthe valve upward when its collar is beyond thecollars of the adjacent valves.

9 Install the replacement valve.

10 Attach the replacement valve's connector to theappropriate location on the electronics board.

11 Reattach the connector for the adjacent valve to theappropriate location on the electronics board.

12 Reinstall the syringe as described in mounting newsyringe on page 4-12.

Replacing Parts

4-14

Maintenance 4

Replacing One or More Probes

Refer to the diagram and appropriate instructionsbelow.

Installing the same type of probe

1 Remove the transfer tubing’s 1/4”-28 fittingconnected to the top of the probe holder.

2 Grasp the current probe and push it up throughthe top of the probe holder.

3 Install the new probe by pushing it through thetop of the probe holder. Make sure the tip of theprobe sits inside the probe guide.

4 Replace and tighten the 1/4"-28 fitting.

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4-15

Maintenance 4

Installing different type of probes

To install replacement probes of a different typethan are currently installed, you must obtain a probeholder/guide kit and the replacement probes.

1 Remove the transfer tubing fittings connected tothe top of the probe holder.

2 Remove each probe by pushing it up throughthe top of the probe holder.

Note: If the liquid level-sensing cable isattached to the probe holder, disconnect it fromthe probe holder.

3 Remove the screws that attach the current probeholder to the Z-arm. Install the new probeholder.

4 Remove the screws that attach the currentprobe guide to the Z-arm foot. Install the newprobe guide.

5 Install each new probe by pushing it throughthe top of the probe holder. Make sure the tip ofeach probe sits inside the probe guide.

6 Reattach the transfer tubing to the probeholder.

7 Reattach the liquid level-sensing cable (optional)to the probe holder.

Replacing Parts

4-16

Maintenance 4

Replacing a Fuse

A blown fuse may indicate the existence of anotherproblem in the instrument. If the replacement fusesblow, don’t try others. Contact your localrepresentative or Gilson. See the before calling ussection on page 5-13.

To change a fuse, follow these steps.

1 Disconnect the power cord from the poweroutlet and from the rear panel receptacle.

2 Locate the fuse drawer on the rear panel. Seepage 2-15 if necessary.

3 Insert a small screwdriver into the notch next tothe fuse drawer.

4 Twist the screwdriver to open and remove thefuse drawer. The fuse drawer contains one2.5A “T” Slo-Blo fuse (5 x 20 mm size) for a100/120 voltage selection. It contains two 2.5Afuses for a 220/240 voltage selection.

5 Remove the old fuse(s) and insert the newfuse(s).

6 Insert the fuse drawer into its receptacle in theliquid handler.

Rep

laci

ng P

arts

4-17

Maintenance 4

Checking Position AlignmentThe SET_215.EXE program, described in Section 3,allows you to test whether the liquid handler isproperly aligned and to make minor adjustments tothe X-axis and Y-axis offsets if needed. To check thecurrent alignment and make adjustments asneeded, select the Adjust X&Y option from themenu. The four items available in this option aredescribed below.

Note: You can also use the 215 Setup Utilityprogram to adjust the X- and Y-axis offsets ifneeded. Refer to its online help.

Items 3 or 4 within this menu should be run first tocheck the initial X and Y coordinates. Then you canmodify the X- and Y-axis offsets using items 1 and2, respectively.

1 Alters the X-axis offset in millimeters.

2 Alters the Y-axis offset in millimeters.

If you changed the X-axis or Y-axis offset, theliquid handler will store the new coordinates.

3 Homes the liquid handler if it is not alreadyhomed and moves the probes to the installedrinse site. The Z-arm becomes relaxed so thatyou can manually move the probes to visuallycheck the alignment with the rinse station.Pressing any key homes the liquid handler.

4 Prompts you for a target location in the X-axisand then a target location in the Y-axis. Theleft-most probe moves to this target locationand the Z-arm is relaxed so that you canmanually move the probes for visual alignmentconfirmation on a target site. Pressing any keyre-homes the liquid handler.

Checking Position A

lignment

4-18

Maintenance 4

Transporting the Liquid HandlerWhen moving the liquid handler to anotherlocation or when sending it back to the factory, donot use the Y-arm as a handle. Reinstall thearmlock (see Section 2) and always lift the liquidhandler from the base.

Tran

spor

ting

the

Liqu

id H

andl

er

5-1

5Troubleshooting

5-2

Troubleshooting 5

Error Codes

5-3

Troubleshooting 5

Error Codes

5-4

Troubleshooting 5

Erro

r Cod

es

5-5

Troubleshooting 5

Error Codes

5-6

Troubleshooting 5

Mechanical

Probe no longer finding tube center

Probe may be bent. Straighten or replace the probe.

Incorrect tray file defined. Review and if necessarychange the tray file using the Tray Editor. See 709Sampler Manager User’s Guide.

Liquid handler may be misaligned. Perform theposition alignment procedures, described on page 4-17.

Mec

hani

cal

5-7

Troubleshooting 5

Electrical

Input functions not operating

Make sure connections into terminal blockconnector are secure.

Make sure terminal block connector is secure ininput/output port.

Check connections for proper pin assignments.

Be sure pins from external devices are assignedcorrectly.

Check polarity of input. Inputs should be a contactclosure. If not, it must be TTL level (logic Øactivates).

Confirm that source supplying input to liquidhandler is working.

Output functions not operating

Make sure connections into terminal blockconnector are secure.

Make sure terminal block connector is secure in theinput/output port.

Check connections for proper pin assignments.

Output from liquid handler should be compatiblewith device to which it is interfaced. Outputs arecontact closures.

Electrical

5-8

Troubleshooting 5

Unit not operational

Make sure power is turned on.

Check AC power cord connections.

Try different AC outlet.

Check fuse(s); replace if necessary.

Check all liquid handler connections and makesure that the unit is plugged in.

Unit blows fuses

Contact Gilson Customer Service (within the U.S.)or your Gilson representative. Refer to page 1-6 forCustomer Service contact information.

Elec

tric

al

5-9

Troubleshooting 5

Tubing and Syringe Pump

Syringe pump will not draw in reagent

Make certain all fittings are tight.

Check valve fitting threads on the syringe pump.Replace if damaged.

No fluid being dispensed

Make sure the syringe's fitting is securely connectedto the solenoid valve retainer.

If tubing is kinked or blocked, replace defectivetubing.

Make sure a solenoid valve is not damaged.Replace if necessary. Turn the power to the liquidhandler off and then back on to re-initialize.

Reagent being pulled back through inlettubing into reservoir


Air gap breaks up

When aspirating a liquid, if the air gap breaks up,check to see if the tubing is the correct size.

Increase size of air gap.

Reduce aspiration flow rate.

Clean or replace any dirty tubing.


5-10

Troubleshooting 5

Syringe bubbles

Make sure that all tube fittings are tight and air-free.

Make sure the syringe is tightened into the valveretainer block.

Clean the syringe if dirty. Refer to Section 4.

If any of the valve fittings are damaged, replace thevalve.

Fluid leak

Make sure fittings are tight.

Replace any worn piston seals on the syringepiston. Instructions are supplied with replacementseals. (Condensation within a syringe indicates aworn piston seal.)

Allow reagents to warm to room temperaturebefore using.

Incorrect aspirating and dispensing

Check for leaks on all fittings and tubes leading tothe probes.

Tighten or replace fittings on inlet and transfertubing as needed.


Syringe stalls

If a syringe stalls, there may be a blockage in thetubing or valve. Inspect all tubing and the valve.

If a syringe stalls due to an accelerated aspirate ordispense flow rate, reduce the flow rate in theprogram being run by the liquid handler.

Tubi

ng a

nd S

yrin

ge P

ump

5-11

Troubleshooting 5

Poor accuracy

Worn piston seals and tubing can cause the liquidhandler to perform with poor volumetric accuracyand precision. (Condensation within a syringeindicates a worn piston seal.)

If the aspirate and dispense flow rates are too fast,slow down the flow rates to adapt to the tubingand probe type.


5-12

Troubleshooting 5

Liquid Level Detector (if installed)

Liquid level detector not detecting liquid level

Note For a multiple probe liquid handler, liquidlevel-sensing only works for the first probe (left-most position) in the probe holder.

Ensure that the level-sensing cable is plugged in.

Check sensitivity setting in SET_215.EXE or the 215Setup Utility program.

Check if liquid is detectable. Liquid level detectionworks only if there is electrical conductivity in yourliquid. Liquid level detecting will not work withmost non-polar liquids. For intermediate polarityliquids and polar liquids, check the sensitivitysetting in SET_215.EXE or the 215 Setup Utilityprogram.

Check the Move Probe command in the 709 SamplerManager Software. The height statement may beoverriding liquid level detecting. See software’suser’s guide.

If none of the above correct the problem, the likelycause is faulty circuitry. Contact Gilson CustomerService (within the U.S.) or your Gilsonrepresentative.

Liqu

id L

evel

Det

ecto

r (if

inst

alle

d)

5-13

Troubleshooting 5

Repair and Return Policies

Before calling us

Gilson Customer Service personnel will be able toserve you more efficiently if you have the followinginformation:

• the serial number and model number of theequipment involved. The serial number is visibleon the back of the control panel of the liquidhandler.

• the installation procedure you used• list of concise symptoms• list of operating procedures and conditions you

were using when the problem arose• list of other devices connected to the liquid

handler and a description of those connections• list of other electrical connections in the room

Warranty repair

Units covered under warranty will be repaired andreturned to you at no charge. If you have anyquestions about applicability, please contact GilsonCustomer Service or your authorized representative.

Non-warranty repair

For out-of-warranty repairs, contact your localGilson representative or Gilson Customer Service.A Customer Service representative will discussservice options with you and can assist in makingarrangements to return the equipment, if necessary.

Rebuilt exchange

For some units, rebuilt exchange components areavailable. Contact Gilson for details.

Repair and R

eturn Policies

5-14

Troubleshooting 5

Return procedure

In the United States, contact Gilson CustomerService to obtain authorization before returningany Gilson equipment. To return a piece ofequipment:

• Carefully pack the unit to prevent damage intransit. Check with Gilson regarding propermethod of shipment. No responsibility isassumed by Gilson for damage caused byimproperly packaged instruments. Indicate theauthorization on the carton and on the packingslip.

• Always insure for the replacement value of theunit.

• Include a description of symptoms, your name,address, phone number and purchase order tocover repair costs, return and shipping charges,if your institution requires it. Ship to:

Gilson, Inc.Attention: Customer Service(indicate the authorization here)3000 W. Beltline HighwayMiddleton, WI 53562-0027

Outside the United States, contact your Gilsonrepresentative for return procedures.

Rep

air a

nd R

etur

n Po

licie

s

A-1

A

For part numbers for syringe piston seals, contact the Gilson Customer ServiceDepartment at 800-445-7661 or 836-1551. Outside the United States, contact your localGilson representative.

Control Software2106773531 735 Sampler Software running directly from PC;

requires PC with Microsoft Windows NT and serialcable.

2106243 709 Sampler Manager Software running directly fromPC; requires PC with Microsoft Windows (V3.1 orhigher) and serial cable.

2106243 709 Sampler Manager Software running on an XLKeypad; requires PC with Microsoft Windows (V3.1or higher) and XL Keypad (270230).

210646 UniPoint LC System Software. Multiple HPLC systemcontrol with data analysis for PC-based systems (IBMor compatible). Requires Microsoft Windows NT and506C System Interface (362831)

21064621 UniPoint System Software. Multiple HPLC systemcontrol with data analysis for PC-based systems (IBMor compatible). Requires Microsoft Windows 98 and506C System Interface (362831)

Replacement Parts and Accessories

A-2

Appendix AR

epla

cem

ent P

arts

and

Acc

esso

ries Probes for 125 mm Z-Arm

27067361 Non septum-piercing probe; bevel tip, capacitivelevel-sensing, stainless steel. Dimensions: 220.5 x 1.5x 1.1 mm ID. Compatible with 889 Multiple InjectionModule and 849 Multiple Injection Module; requiresinjection port bar (part number 250512753 for the889 or part number 250512863 for the 849). Requiresprobe holder/guide kit (part number 253652).

2507414 Non septum-piercing probe; constricted tip, capacitivelevel-sensing, stainless steel. Dimensions: 220 x 1.3 x0.8 mm ID (tip dimensions: 1.5 x 0.9 x 0.45 mm ID).Compatible with 889 Multiple Injection Module and849 Multiple Injection Module; requires injection portbar (part number 250512752 for the 889 or partnumber 250512862 for the 849). Requires probeholder/guide kit (part number 253651).

27067373 Non septum-piercing probe; constricted tip,capacitive level-sensing, stainless steel. Dimensions:221 x 1.5 x 1.1 mm ID (tip dimensions: 2 x 1.1 x 0.4mm ID). Compatible with 889 Multiple InjectionModule and 849 Multiple Injection Module; requiresinjection port bar (part number 250512753 for the889 or part number 250512863 for the 849). Requiresprobe holder/guide kit (part number 253652).

27067374 Non septum-piercing probe; constricted bevel tip,capacitive level-sensing, stainless steel. Dimensions:221 x 1.5 x 1.1 mm ID (tip dimensions: 2 x 1.1 x 0.4mm ID). Compatible with 889 Multiple InjectionModule and 849 Multiple Injection Module; requiresinjection port bar (part number 250512753 for the889 or part number 250512863 for the 849). Requiresprobe holder/guide kit (part number 253652).

2507252 Micro septum-piercing probe; constricted 45° beveltip, capacitive level-sensing, stainless steel.Dimensions: 221.5 x 1.5 x 1.1 mm ID (tipdimensions: 10 x 0.7 x 0.4 mm ID). Compatiblewith 889 Multiple Injection Module and 849Multiple Injection Module; requires injection portbar (part number 250512751 for the 889 or partnumber 250512861 for the 849). Requires probeholder/guide kit (part number 253652).

A-3

Appendix AR

eplacement Parts and A

ccessories

27067377 Grooved septum-piercing probe; beveled-tip,stainless steel. Dimensions: 221 x 1.5 x 0.4 mm ID.Compatible with 889 Multiple Injection Moduleand 849 Multiple Injection Module; requiresinjection port bar (part number 250512753 for the889 or part number 250512863 for the 849).Requires probe holder/guide kit (part number253652).

27067375 Non-septum-piercing probe: beveled-tip, Teflon-coated stainless steel. Dimensions: 221 x 1.5 x 1.1mm ID. Requires probe holder/guide kit (partnumber 253652).

Probes for 175 mm Z-Arm2507215 Non septum-piercing probe; constricted tip, capacitive

level-sensing, stainless steel. Dimensions: 269 x 1.3 x0.8 mm ID (tip dimensions: 1.5 x 0.9 x 0.45 mm ID).Compatible with 889 Multiple Injection Module and849 Multiple Injection Module; requires injection portbar (part number 250512752 for the 889 or partnumber 250512862 for the 849). Requires probeholder/guide kit (part number 253651).

25073645 Non septum-piercing probe; beveled tip, capacitivelevel-sensing, stainless steel. Dimensions: 269 x 1.3 x0.8 mm ID. Compatible with 889 Multiple InjectionModule and 849 Multiple Injection Module; requiresinjection port bar (part number 250512752 for the889 or part number 250512862 for the 849). Requiresprobe holder/guide kit (part number 253651).

Probe Holder/Guide Kits253651 Probe holder/guide kit; includes probe holder and

guide for 1.3 mm outer diameter probes.

253652 Probe holder/guide kit; includes probe holder andguide for 1.5 mm outer diameter probes.

A-4

Appendix AR

epla

cem

ent P

arts

and

Acc

esso

ries Syringes, Transfer Tubing, and Waste Bottle

25053146 100 µl syringe

25053147 250 µl syringe

25053148 500 µl syringe

25053141 1 ml syringe

25053142 2.5 ml syringe

25053145 5 ml syringe

499421202 1.5 ml FEP tubing; 0.8 mm ID x 10 feet

25053173 1.5 ml Teflon transfer tubing assembly, 0.8 mm ID;for use with 100 µl, 250 µl, 500 µl and 1 ml syringeson the Multiple Probe 215. Includes eight bundledand labeled lengths of transfer tubing and fittings.

499471112 5.5 ml FEP tubing; 1.5 mm ID x 9.27 feet; withOmnifit 1/4”-28 fittings

25053174 5.5 ml Teflon transfer tubing assembly, 1.5 mm ID;for use with 2.5 ml and 5 ml syringes on theMultiple Probe 215. Includes eight lengths ofbundled and labeled transfer tubing.

F1410050 PVDF coupling for 1/4”-28 fitting, package of 5

25053172 Waste bottle (8 liter) with lid and quick-connectfitting

470343706 Tygon tubing (5/16” ID x 7/16” OD) forconnection between rinse station and waste bottle;per foot

23077332 Quick-connect fitting to connect Tygon tubing towaste bottle

A-5

Appendix AR

eplacement Parts and A

ccessories

Rinse Station25045525 Multiple Probe 215 rinse station; connects to rinse

station base

25245512 Rinse station base; attaches directly to 215 locatorplate

Rack AccessoriesFor part numbers for available racks, refer to Appendix B. To create your own code200-style rack, order the blank rack kit (part number 254461) and rivet gun (partnumber 4391002).

Cables and I/O Accessories36083121 Serial cable, IBM PS/2-type, 25 to 25 pin36083122 Serial cable, IBM AT-type, 9-pin female to 25-pin

male36083123 Serial cable adapter, 9-pin female to 25-pin male638308512 Terminal block connector, 8-pin638310512 Terminal block connector, 10-pin709910206 2-conductor interconnect wire, 6’, for making

contact connections36078143 Shielded GSIOC cable, 30”6730254007 2.5A, T-2.5 Slo-Blo fuse

Miscellaneous25064040 Level-sensing cable23077333 Y-connector to connect two rinse stations to one

waste bottle2509211 Armlock with hex screw4311403 9/64” ball driver (hex wrench for armlock)54118025 Spiral wrap

A-6

Appendix AR

epla

cem

ent P

arts

and

Acc

esso

ries Probe/Injection Port Compatibility Chart

for the Multiple Probe 215 Injector(with 889 Multiple Injection Module)

Probe/Injection Port Compatibility Chartfor the Multiple Probe 215 Injector(with 849 Multiple Injection Module)

B-1

B

This appendix describes the racks that can be purchased for use on the Multiple Probe 215Liquid Handler.

Code 201 rack

For two microplatesMaterial: aluminumVessels and maximum capacity: 96-wellmicroplatesPart number: 2504601

Code 201H rack

For two microplates with hold-down coversMaterial: aluminumVessels and maximum capacity: 96-wellmicroplatesPart number: 2504601H

Racks

B-2

Appendix BR

acks Code 205 rack

For two deep-well microplatesMaterial: aluminumVessels and maximum capacity: 96 deep-wellmicroplatesPart number: 2504605

Code 205H rack

For two deep-well microplates with hold-downcoversMaterial: aluminumVessels and maximum capacity: 96 deep-wellmicroplatesPart number: 2504605H

Code 217 rack

For 96 tubesMaterial: aluminumVessels and maximum capacity: 96 10 x 75 mm(3.5 ml) culture tubesPart number: 2504617Note: The zigzag pattern allows all eight probes todraw from one row of test tubes.

B-3

Appendix BR

acks

Code 218 rack

For two microplates and eight 10 x 75 culture tubesMaterial: aluminumVessels and maximum capacity: two 96-wellmicroplates and eight 10 x 75 mm culture tubes(3.5 ml)Part number: 2504618Note: Instead of microplate(s), you can installBeckman modular reservoirs.

Code 228 rack

For four reagent bottlesMaterial: aluminumVessels and maximum capacity: 4 (500 or 700 ml)Part number: 2504628

Code 505 rack

For ten standard or deep-well microplatesMaterial: aluminumVessels and maximum capacity: ten 96-wellmicroplatesPart number: 2504651

Code 505H rack

For ten standard or deep-well microplateswith hold-down coversMaterial: aluminumVessels and maximum capacity: ten 96-wellmicroplatesPart number: 2504651H

C-1

C

This appendix contains information on the methods used to obtain the volumetricprecision and accuracy specifications and the carryover specification listed in Section 1.

Volumetric Precision and Accuracy MethodsVolumetric precision and accuracy data was obtained gravimetrically. A volume ofdegassed tap water was dispensed in a source tube.

All gravimetric data was obtained using a Sartorius AC 210P four-place balance(#20201203).

Details of each of the methods used follows.

Specifications Methods

C-2

Appendix CSp

ecifi

catio

ns M

etho

ds 50 µl Sample : 500 µl Syringe

Probes capacitive, non-septumpiercing probes(part number 27067373)

Transfer tubing 1.5 ml FEP tubing, 0.8mm ID x 10 ft. (partnumber 030723)

Sample degassed tap water

Type of transfer dispense

Tube size 10 x 75 mm culture tubesin Code 217 rack

Syringe size 500 µl

Pump aspirate speed 6 ml/min

Pump dispense speed 6 ml/min

Prime speed 80 ml/min

Number of tubes 24

Dispense height 0 mm

Sample volume 50 µldelivered

Rinse volume 1000 µl

Actual tube ID 10.6 mm

Total dispense time 11.6 seconds

Additional notes

Sample was dispensed into empty tubes. Therefore,no evaporation factor tube was used because emptytubes were targeted.

Delivery was 10% of syringe size.

C-3

Appendix CSpecifications M

ethods

500 µl Sample : 500 µl Syringe

Probes capacitive, non-septumpiercing probes (partnumber 27067373)





Amount initially in 0 µltarget





Number of tubes 24





Total dispense time 32 seconds

Additional notes



C-4

Appendix CSp

ecifi

catio

ns M

etho

ds 100 µl Sample : 1000 µl Syringe











Number of tubes 24






Additional notes



C-5

Appendix CSpecifications M

ethods

1000 µl Sample : 1000 µl Syringe











Number of tubes 24






Additional notes



C-6

Appendix CSp

ecifi

catio

ns M

etho

ds Carryover MethodUsing a Multiple Probe 215 Liquid Handlerequipped with a capacitive level-sensing, septumpiercing probe, carryover was measured using a testsolution of anthracene (50 µg/ml) in methanol.Transfers were done in a series of tubes startingwith the 50 µg/ml standard of anthracene followedby 3 blanks of methanol. A rinse of 500 µl methanolwas performed between each transfer step.

Concentrations in the tubes were then measured byHPLC fluorometry. Blank source tubes and targettubes were measured.

D-1

D

The Gilson Serial Input Output Channel (GSIOC) is an asynchronous serialcommunications interface that enhances the power of your Gilson equipment.

The GSIOC incorporates an EIA RS-485 interface and allows up to 32 slave devices to becontrolled from a single master in a multi-drop configuration.

Each slave device is identified by a unique number which must be known to the deviceand to the controller. The default unit ID of the liquid handler is 22.

To control the liquid handler via the GSIOC interface, you need:

• a Personal Computer with Microsoft Windows 98 or NT and any Gilson controlsoftware or 706 Device Driver Software installed

• an unused RS-232 communication port

Using the computer and software, you:

• specify the liquid handler as the device you want to control• issue commands that set operating parameters, control operation, or request

information from that device.

GSIOC Commands

D-2

Appendix DG

SIO

C C

omm

ands GSIOC Commands

There are two kinds of commands that you cansend over the GSIOC:

• Buffered commands send instructions to theliquid handler. These commands are executedone at a time.

• Immediate commands request status informationfrom the liquid handler. These commands areexecuted immediately, temporarily interruptingother commands in progress.

GSIOC Command List

In the command list below, the GSIOC commandmust be entered in the proper upper or lower caseformat. If a buffered command requires additionalinformation, you’ll see italicized text next to thecommand. The description of the commandidentifies what you need to enter in place of theitalicized parameter. Also note that if a parameteris optional, it appears within brackets, [ ].

I - ImmediateB - Buffered

Command Type Description

% I Identifies the selected slave device. Returns charac-ter string: “215MULVx.yz”, where x, y, and zrepresent software version.

$ I Resets the liquid handler.

@ I Reads non-volatile memory (NV-RAM) at currentaddress. Returns “aa=xxxx” where:

aa - Value between 0 and 19 for word type dataor 100 to 119 for floating type data.

xxxx - Data at the address.

Current address is incremented.

D-3

Appendix DG

SIOC

Com

mands

@aa[=xxxx] B Sets the value at NV-RAM address where:

aa - Value between 0 and 19 for word typedata or 100 to 119 for floating type data.

xxxx - (Optional) Data at the address.

~n B Sets test mode depending on value assigned to n:1 - XYZ test.2 - Syringe pump test.3 - Display liquid level sensor frequency.8 - Disable XY phase checking.9 - Reset NV-RAM and initialize to defaults.

9 I Reads contact input event FIFO. Returns “Xtttttt”where:

X - State of the four contact inputs: 1 forclosed, 0 for open. See table below.

tttttt - Time since the last buffered 9 command

X A B C D@ 0 0 0 0A 1 0 0 0B 0 1 0 0C 1 1 0 0D 0 0 1 0E 1 0 1 0F 0 1 1 0G 1 1 1 0H 0 0 0 1I 1 0 0 1J 0 1 0 1K 1 1 0 1L 0 0 1 1M 1 0 1 1N 0 1 1 1O 1 1 1 1

Whenever the status of a contact input changes, thestate of all contacts and the time since the last buffered9 command in the event FIFO is encountered.

D-4

Appendix DG

SIO

C C

omm

ands 9 B Clears the contact event FIFO and resets the

contact input event timer.

A I Reads home phases for X and Y motors. Returns“Xphase/Yphase” where:

xxx - X motor phase.yyy - Y motor phase.

Bf,d B Causes the liquid handler to beep:

f - Frequency in Hz; default is 2400.d - Duration of beep in tenths of seconds;

default is 1.

cv=ss..ss B Sets user-definable character:

v - Address (0 to 7) of user-definedcharacter.

ss..ss - Characters, in hexadecimal ASCII code,for rows 1 through 7. See examplebelow.

For example, the following command sets thepattern shown below to user-defined characteraddress 0: c0=1F111B151B111

[Row] [Pattern] [Hex Code]1 * * * * * 1F2 * * 113 * * * * 1B4 * * * 155 * * * * 1B6 * * 117 * * * * * 1F

D I Reads syringe pump status. Returns “vxxx” where:

v - Valve position: N if all 8 valves are inprobe position, R if all 8 valves are inreservoir position, or "–" if all 8 valves arenot in the same position.

xxx - Current volume in µl in syringes.

d B Homes syringe pump. Sends the piston to the upperposition with all 8 valves in the probe position.

D-5

Appendix DG

SIOC

Com

mands

Ddvvv[,ss][,Zzz] B Sets syringe pump parameters:

d - Valve position for all 8 valves: N if inprobe position, R if in reservoir position.

vvv - Signed decimal volume in µl.ss - (Optional) Syringe speed in ml/min.zz - (Optional) Z-arm speed in mm/sec. Note

that valve must be in probe position touse this parameter.

e I Reads the current error number. Returns “nnn”which identifies the error number; see page 5-2 forlisting of errors. If no error has occurred, returns 0.

e B Clears error number.

Exyz B Sets X, Y, and Z motor status:

x - 0 for disable or 1 for enable X motor.y - 0 for disable or 1 for enable Y motor.z - 0 for disable or 1 for enable Z motor.

For example, the following command disables themotors: E000.

F I Returns maximum flow rate in ml/min for syringepump's syringes.

H[Z] B Moves probes to home position.

Z - (Optional) Homes Z only

I I Reads status of input contacts and front panelStart and Stop buttons. Returns “cccckk” where:

cccc - Status of input contacts A, B, C, and D: 1if closed (shorted), 0 if open.

kk - Status of front panel Start and Stopbuttons: 1 if button has been pressed, 0 ifit hasn’t been pressed. Value of kk iscleared after reading.

D-6

Appendix DG

SIO

C C

omm

ands J I Reads status of output contacts. Returns “cccckk”

where:

cccc - Status of output contacts 1, 2, 3, and 4: 1if connected, 0 if disconnected.

kk - Status of keypad and auxiliary power: 1 ifconnected, 0 if disconnected.

Jcccc[p] B Sets output contacts and +24 V external power.

cccc - Output contacts 1, 2, 3, and 4: 1 toconnect, 0 to disconnect, X for no change.

p - (Optional) Keypad and auxiliary power: 1to connect, 0 to disconnect, X for nochange.

jcttt B Pulses an output contact:

c - Number of the output contact, 1 - 4.ttt - Duration of the pulse in tenths of seconds;

default is 1.

Lx B Sets liquid level-sensing threshold frequency basedon current frequency and data at NV-RAMfrequency threshold field.

x - H for high frequency setting or L for lowfrequency.

M I Reads X, Y, Z motor status and syringe pumpstatus. Returns “xyzp”. For each status, you’ll see Ufor unpowered, P for powered, R for running, or Efor error.

N I Reads the liquid level detector output. Returns“Innnn” where:

I - A for air or L for liquid.nnnn - Current sensitivity threshold frequency in

Hz.

Nffff B Sets the liquid level-sensing threshold frequency(ffff) is the frequency in Hz.

D-7

Appendix DG

SIOC

Com

mands

n I Reads the actual frequency of liquid detectionoscillator. Returns “ffff” which is frequency in Hz.

Q I Reads the XYZ travel range. Returns “axis=min/max”where:

axis - X, Y or Z.min - Lowest position in tenths of millimeters.max - Highest position in tenths of millimeters.

The first time the command is sent, it returns the Xrange; the second time, the command returns the Yrange; the third time, the command returns the Zrange.

R I Reads front panel LED display. Returns “cccccccc”which is the 8 characters currently shown on thedisplay. If a character is non-printable, thehexadecimal ASCII code for the character isreturned.

S I Reads the command in the synchronization buffer.Returns “|” if the buffer is empty.

Smm B Sends a synchronized buffered command (mm) thatwill be executed when the instrument is quiescent.Sending a command can overwrite unexecuted,existing commands. If you send this commandwithout indicating a parameter (mm), the buffer iscleared.

V I Reads the status of the syringe pump's 8 valves.Returns one of the following codes for each of the 8valves: N for probe position or R for reservoirposition.

For example, if “NNNNNNNN” is returned, all 8valves are in the probe position.

Note: Before you issue this command, you musthome (initialize) the syringe pump using the buffered“d” command.

D-8

Appendix DG

SIO

C C

omm

ands Vabcdefgh B Sets the position of the syringe pump's eight valves.

For abcdefgh, indicate one of the following:

N - Probe positionR - Reservoir position

For example, the command VNNNRRRxx turnsvalves 1, 2, and 3 to the probe position, turns valves4, 5, and 6 to the reservoir position, and leavesvalves 7 and 8 in their current position.

Note: Before you issue this command, you musthome (initialize) the syringe pump using the usingthe buffered “d” command.

Wcc..cc B Writes character string (cc..cc) to the LED display.Characters outside the printing range are shown inhexadecimal ASCII code on the display. Userdefinable characters are available with codes of 80to 8F.

X I Indicates the location of the probe in the X-axis andY-axis. Returns “xxxx/yyyy” which is X and Yposition in tenths of millimeters.

x I Indicates X motor status. Returns one of thefollowing: U for unpowered, P for powered, R forrunning, or E for error.

Xxxxx[/yyyy] B Sets new X and Y positions where:

xxxx - X position in tenths of millimeters.yyyy - Y position in tenths of millimeters.

Xxxxx[,s] B Sets new X position where:

xxxx - X position in tenths of millimeters.s - (Optional) Speed index of 0 or 1; default

is 0.

s Speed0 Normal speed 39.29 cm/sec1 Slow speed 7.06 cm/sec

Y I Indicates the location of the left-most probe in theY-axis. Returns “yyyy” in tenths of millimeters.

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Appendix DG

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y I Indicates Y motor status. Returns one of thefollowing: U for unpowered, P for powered, R forrunning, or E for error.

Yyyyy[,s] B Sets new Y position where:

yyyy - Y position in tenths of millimeters.s - (Optional) Speed index of 0 or 1; default

is 0.

s Speed0 Normal speed 39.19 cm/sec1 Slow speed 6.71 cm/sec

Z I Indicates the location of the left-most probe in theZ-axis. Returns “zzzz” in tenths of millimeters.

Zzzzz[,s] B Sets new Z position where:

zzzz - Z position in tenths of millimeters.s - Speed index of 1 to 5; default is 4. See

table.

s Speed (cm/sec)1 2.0612 2.9633 8.6224 16.8545 29.799

z I Indicates Z motor status. Returns one of thefollowing: U for unpowered, P for powered, R forrunning, or E for error.

zppp[,s] B Indicates the movement in the Z-arm. Stops whenthe liquid level has changed where:

pppp - Z position in tenths of millimeters.s - Speed index of 1 to 5; default is 2. See

table above.

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E

Follow the techniques described in this chapter to maximize accuracy and precision, andminimize carryover with your liquid handler.

The accuracy and precision of pipetting depend on the specifications of your pump andthe geometry of your probe tip.

Please refer to Appendix A for a listing of probes available for the Multiple Probe 215.

Pipetting Techniques

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s Minimizing Cross-ContaminationCross-contamination of samples is caused bysample carryover on the probe tip or on its innerwalls. Several methods are available to minimizecross-contamination.

• Use of the liquid level-sensing capability of theliquid handler will reduce carryover by limitingimmersion below the liquid surface.

• Cleansing the probe tip with a flow-throughrinse removes contaminants from the externalsurfaces of the probes.

• Dispensing liquid through the probes into therinsing station will eliminate internalcontamination.

An alternate internal/external rinsing using a non-flowing rinse is also possible. (Internal rinsing maynot be required at all if large diluent volumes aredispensed immediately after dispensing of thesample – a common practice in dilution procedures.)

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Appendix EPipetting Techniques

Minimizing Dilution EffectAn undesirable dilution sometimes occurs when anaspirated sample comes into contact with rinse ordiluent material. Some of the diluent fluidinvariably clings to the tubing walls, causing theunwanted dilution of the sample. The part of thesample that enters the tubing first is affected mostbecause it is the closest to the diluting liquid.

The corrective action you take depends on thestringency of your requirements for sample purityand precision.

• You can aspirate an air gap into the probe tip toseparate different fluids passing through thetubing to reduce mixing.

• You can aspirate an amount in excess of therequired sample volume, dispensing the excesssample, which is most subject to being diluted,to waste.

In extreme cases, you can combine the methodslisted above and perform the following routine toeliminate mixing of different liquids.

1 Move probes to sample tubes.2 Aspirate air gap.3 Lower probes to sample level.4 Aspirate small amount of sample – this is the

excess sample volume.5 Lift probes.6 Aspirate air gap.7 Lower probes to sample level.8 Aspirate sample amount.

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Appendix EPi

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s The following example describes an instance whenthe above 8-step procedure is required:

An operator needs to dispense 50 µl of reagent intoeach row of test tubes in a Code 217 rack. The tube-to-tube consistency of reagent volume is critical.However, in order to save time, the operator wishesto make a single 1 ml aspiration from the reagentreservoir and then make multiple dispenses. If the8-step procedure were not used, the last aliquot ofthe 20 dispenses would be more diluted than theothers. If the procedure was used, the requirementsof the routine and the operator’s desire to save timecould both be met. (Recommended volumes in thisexample are 15 µl air gaps and 100 µl excess volumein Step 4.)

multiple probe 215 liquid handler user's guidefor safe and correct use of the multiple probe...

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