monitoring progress report 2006 food and agriculture (fa) domain committee 3rd dc meeting, antalya...
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MONITORING PROGRESS MONITORING PROGRESS
REPORT 2006REPORT 2006
Food and Agriculture (FA) Domain Food and Agriculture (FA) Domain
CommitteeCommittee
3rd DC meeting, Antalya (TR), 31 Jan – 2 Feb 2007
COST Action 871Domain Food and Agriculture (FA)
Cryopreservation of crop species in Europe
CSO approval date 27/06/2006
Entry into force 20/09/2006
End date 11/12/2010
Participating Countries : 15
BG, CZ, DK, FI, FR, BG, CZ, DK, FI, FR, DE, IT, NL, PL, PT, DE, IT, NL, PL, PT, CS, SK, ES, UK CS, SK, ES, UK
In the pipe-lineIn the pipe-line: : TR, RO, GR, LUTR, RO, GR, LU
Non-COST: Vavoliv, RussiaVavoliv, Russia Economic Dimension: 38 Million €
Chair : BE
Duration: 4 years
Action 871: Cryopreservation of crop species in Europe
Management Report data, part 2 Management Report data, part 2 Chair of the Action:
Dr. Bart Panis: Laboratory of Tropical Crop Improvement, K.U.Leuven,Kasteelpark
Arenberg 13, B-3001 Leuven, BELGIUM Vice chair of the Action:
Professor Paul LYNCHBiological and Forensic Sciences department,,School of Science University of Derby, Kedleston Road DE22 1GB Derby, United Kingdom
Rapporteur:Dr. Charles Spillane:
Genetics & Biotechnology Lab, Biochemistry DepartmentUniversity College Cork, Lee Maltings 2.1, Cork, Ireland
Science Officer:B. STOL
Administrative Officer: C. PEETERS Action website: in preparation
OBJECTIVES
The main objective of this action is to improve and apply technologically advanced techniques for plant genetic resources conservation of crops that are grown/ and or conserved in Europe with main emphasis on long-term conservation through cryopreservation.
Management Report data, part 3 Management Report data, part 3
Objective 1: To screen in detail the current utilization of plant cryopreservation in Europe.
Objective 2: To screen and compare the efficiency of existing plant cryopreservation protocols.
Objective 3: To improve fundamental knowledge about cryoprotection through the determination of physico-biochemical changes associated with tolerance towards cryopreservation.
Objective 4: To develop new plant cryopreservation protocols. Objective 5: To assure the genetic stability and true-to-typeness of
plants after cryopreservation. Objective 6: To apply cryopreservation to European plant
germplasm collections. Objective 7: To proof the environmental, social and economic
impact of plant cryopreservation.
Specific objectives of CRYOPLANET ?
Management Report data, part 3 Management Report data, part 3
WG1Fundamental aspects of
cryopreservation/cryoprotection and genetic stability
WG2Technology, application and
validation of plant cryopreservation
Optimisation Feed back
Management Report data, part 4 Management Report data, part 4
1.1. Fundamental aspect of cryopreservation and cryoprotectionElucidation of the physico-biochemical background of cryoprotection and
cryopreservation.
WG1: Fundamental aspects of cryopreservation/cryoprotection and genetic stability
Water thermalbehavior
Cytoskeletalprotein
Membranecomponents
Proteins Sugars
Polyamines
OxidativestressProfessor Pawel PUKACKI (chair WG1)
Physiology of Abiotic Stress Lab
Institute of Dendrology, Polish Academy of Sciences
Parkowa 5, 62-035 Kornik
Poland
1.2. Genetic stability and authenticity Assessment of the genetic integrity of plants to determine if they are ‘true
to type’ after cryopreservation.
WG1: Fundamental aspects of cryopreservation/cryoprotection and genetic stability
Dr. M. Angeles REVILLA BAHILLO (co-chair WG1)
Departamento Biología de Organismos y Sistemas
Faculty of Biologia, Universidad de Oviedo
Catedrático Rodrigo Uría s/n, 33071 Oviedo
Spain
2.1. Technology aspects of cryopreservationApplications of different cryopreservation protocols to different plant
species and tissues.
WG2: Technology, application and validation of plant cryopreservation
Dr. Florent ENGELMANN (chair WG2)
UR 141, IRD
BP 64501, 911 avenue Agropolis
34394 Montpellier cedex 05
France
2.2. Impact and applications of cryopreservation in plantsgenebanks, establishment of cryo-bank and dissemination of results
WG2: Technology, application and validation of plant cryopreservation
Dr. Joachim KELLER (co-chair WG2)
Leibniz Institute of Plant Genetics and Crop Plant Research IPK
Corrensstraße 3, 466 Gatersleben
Germany
•National Seed Storage Laboratory (NSSL) (Fort Collins, Colorado, USA): 2,100 accessions of apple (dormant buds) •National Clonal Germplasm Repository (NCGR) of Corvallis (USA): 104 accessions of pear (shoot tips);•International Potato Centre (CIP) (Lima, Peru) : 345 potato accessions•Tissue Culture BC Research Inc.(Vancouver, BC, Canada) : 5000 accessions representing 14 conifer species•AFOCEL (Association Forêt Cellulose) of France, with over 100 accessions of elm (dormant buds);•National Institute of Agrobiological Resources (NIAR) of Japan, with about 50 accessions of mulberry.•IRD (Montpellier, France) : 80 accessions of oil palm •IPK (Gatersleben, Germany)/ DSMZ (Braunschweig, Germany) : 519 old potato varieties•INIBAP, Laboratory of Tropical Crop Improvement, K.U.Leuven (Heverlee, Belgium) : 500 banana accessions
State of the art (Europe/rest world)
(i) the unavailability of efficient and robust cryopreservation protocols applicable to many plant species and diverse germplasm types
(ii) limited awareness of plant researchers unacquainted to recent developments in cryogenic storage methods
(iii) lack of coordinated research on plant cryopreservation.
COST action like “CRYOPLANET” can make the difference
Why is cryopreservation not more widely applied?
Time table
Year 1 Year 2 Year 3 Year 4
Coordination
Kick-off meeting
Homepage
Reporting
MC meeting
WG1 meeting
WG2 meeting
Workshop*
STSMs
Final Conference
MC meeting: Management committee meeting; WG meeting: Working group meeting; STSMs: Short-term scientific missions; Workshop*: Timing of the Inter-COST Workshops will be defined in agreement with the Management committee of that specific Action.
Meetings plannedMeetings planned1/ WG1 meeting
April 13-14 Oviedo (Spain)Organiser Dr. M. Angeles REVILLA BAHILLO
2/ WG2 meeting combined with meeting of executive committee May 11-12 Firenze (Italy)Organiser Dr. Lambardi MAURIZIO
3/ Management Committee meeting In combination with the Society for Low Temperature Biology Annual Scientific meeting at the University of Derby (12-14th Sept 2007) hosted by Professor Paul LYNCH
4/ Training Workshop on DSC and thermal analysis (8 researchers)End 2007Organiser Dr. Milos FALTUS (Prague, Czech Republic)
• Articles in refereed scientific journals• Common reviews, books• A public website (information about the project, the achievements,
services/consultancies offered and announcements of training workshops.
• Information on the official webpages of the collaborating institutions.
• The consortium will organize workshops for scientists, germplasm curators regulatory bodies and policy makers
• At the end of the Action, the consortium will offer its expertise as a service to the EU.
• Presentations at International Conferences, for promoting the European know-how and increasing the international collaboration.
• Teaching activities in Universities at undergraduate and post-graduate level. Young scientists and engineers will thus be trained and informed on the latest developments in cryopreservation.
Significant need to develop dissemination plan. This will be a major agenda item at Executive
Committee Meeting in May (Firenze). Members of COST 871 need to feedback ideas to the
Executive Committee by end of April 2007
Dissemination plansDissemination plans