Mohiuddin_Figure S1

10 min 2 h

A

B

10 min 2 h 5 h

Mohiuddin_Figure S2

MfeIBamHI

MARCOTR

pTOPO

Digest with BamHI & MfeI

Digest with BglII & EcoRI

BglII EcoRI

IRES GFP

pMSCV

AID

BglII EcoRI

IRES GFP

pMSCV

MARCOTR

BamHI MfeI

BglII EcoRI

IRES GFP

pMSCV

MARCOTR

B

Human cells (293T cells)

Genetically modified Retroviral expression vectorHelper plasmid (pCLampho)

Viral supernatantWT, △FEN1 &

△ REV3 DT40 cells

WT cells with chimera,△FEN1 cells with chimera &△REV3 cells with chimera

C

A

TR

BamHI

AvrII

P1

P2

MARCO

AvrII

MfeI

P3

P5

TOPO Cloning

TRBamHI AvrII MARCO

BamHI

MfeI

TOPO Cloning

AvrII

BamHI

Digest with BamHI & AvrII

Digest with BamHI & AvrII

MARCO

BamHI

MfeIAvrIITR

BamHI

AvrII

Ligate

AvrII

pTOPO

pTOPO

pTOPO

MfeIBamHI

MARCOTR

pTOPO

Digest with AvrII

TR MARCOC C T A G GG G A T C C

SI Nuclease

TR MARCOC GG C

Ligate

BamHI

MARCOTR

pTOPO

MfeI

AvrII

P4

Mohiuddin_Figure S3

Mohiuddin_Figure S4

WT

WT + Beads

Green Fluorescence

Red Fluorescence

Forward Scatter

Sid

e S

catte

r

Cou

nt

24 h after exposure

WT with Long Chimera

WT with Long Chimera +

Beads

WT with Short Chimera

WT with Short Chimera +

Beads

Green Fluorescence

Red Fluorescence

Forward Scatter

Sid

e S

catte

r

Cou

nt

24 h after exposureA

Mohiuddin_Figure S4 (continued)

B

WT

WT + Beads

Green Fluorescence

Red Fluorescence

Forward Scatter

Sid

e S

catte

r

Cou

nt

3 h after exposure

WT with Long Chimera

WT with Long Chimera +

Beads

WT with Short Chimera

WT with Short Chimera +

Beads

Green Fluorescence

Red Fluorescence

Forward Scatter

Sid

e S

catte

r

Cou

nt

3 h after exposure

WT WT with Short Chimera

WT with Long Chimera

Without Beads

With Beads

C

Table S1: List of primers used to make chimeric receptor.

Name Sequence

P1 (+) ATTAGGATCCATGGATCATGCCAGAGCAGCATTGTCTAAC

P2 ( -) ATTACCTAGGAAGATGACAGGCGGTCCTTGAATCTC

P3 (+) ATTACCTAGGGACACAAAGGAGAACGCGGTCC

P4 (+) ATTACCTAGGTGGAAATCTTTCACCAAGGGTCCTGG

P5 (- ) ATTACAATTGTCAGACACACTCCACGCCGGCGTC