Module „NMR“ (MSc): Practical exercises Sheet 02 2.1

2.2

2.3

2.4 Read the following publication and explain the relevance of the following NMR data:

A. Lange, S. Becker, K. Seidel, K. Giller, O. Pongs and M. Baldus (2005) A concept for rapid

protein-structure determination by solid-state NMR spectroscopy. Angew Chem. Int. Ed. 44,

2089–2092. Supporting information is available online.

• Solid-state NMR applications on fully/partially immobilized systems (biomolecules).

Q1: Why solid-state NMR is employed on those biomolecules?

Q2: How was the so-called ‘rapid’ structural characterization carried out?

Q3: How were the dihedral angles and distance constraints deduced from the correlation

spectra?