modulating the interactions of nanoparticles with ... · pdf file- common chemical techniques...
TRANSCRIPT
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Modulating the Interactions of Nanoparticles with Biomolecules through appropriate
Surface Modifications
Prakash D. Nallathamby, Ph.D
Department of Aerospace and Mechanical Engineering
Bioengineering Program
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37 nm 104 nm
2 nm 31 nm
G. Metal Oxide Nanoparticles (e.g. Gd2O3)
E. Anisotropic Patchy Silica NPs
H. Fluorescent NanoparticlesLSPRSO
LSPRSO
RBITCO
FITCO
I. X-ray Contrast Agent (Spectral CT)
F. Core shell Nanoparticles (Variable shell thickness)
20 nm
20 nm 100 nm
J. MRI Contrast
II. Surface Modifications
PEI silane
EDTA silane
Acetic acid silane
Mixed monolayer of thiols
I. Nanoparticle Synthesis
B. Iron Oxide Magnetic Nanoparticles
100 nm
C. Porous Silica Nanoparticles
A. Plasmonic Nanoparticles (e.g. Ag)(Color as a size index- optical nanorulers)
100 nm20 nm
100 nm
20 nm
D. Nanorods (Au) Nanoplates (Gd)
doi:10.1039/c4nr06441k
10.1039/c3nr02639F
10.1039/C6TB01659F
doi:10.1039/C6TB01659F
doi:10.1557/opl.2015.460
doi:10.1039/C6TB01659F
doi:10.1039/c4nr06441k
doi:10.1021/ac0709706
Major Areas of Focus
doi:10.1039/c0nr00303d
III. Optical Lithography based Microfabrication
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Surface Modifications Are Necessary to Immobilize Nanoparticles on Varied Materials
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Surface Modifications Are Necessary to Attenuate Probe Toxicity
Chem. Res . Toxicol., 2013 , 26 (6), pp 904 –917
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Probes with Low toxicity Were Used to Monitor Live Zebrafish Embryos for 72h
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Surface Modifications to Bait-and-Switch Microbes
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Non- Modified Nanoparticles are Immediately Ejected by Bacteria
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Surface Tuned Nanoparticles Accumulate in Bacteria
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Surface Modifications to Mask Systemic Toxicity
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Outline of the talk• Section I
- Common chemical techniques employed to modify surface of nanoparticles
• Section II- Characterization of chemical functional groups on nanoparticles
• Section III- Common conjugation techniques
• Section IV- Published examples of how surfaces are modified to meet end user needs
• Section V- Interesting reads
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Common Molecules for Surface Modifications
• Thiols (-SH)• Applicable to modifying gold, silver, copper and thiol
reactive metals like molybdenum
• Silanes (Orthosilicates)• Applicable to modifying glass, silica and oxide surfaces
that have been treated to overexpress –OH groups
• Polymer Meshes• Polyvinyl pyrrolidone (PVP), polyethyleneimine (PEI)
and fatty Acids (glycerolmonooleate)
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Sulfur Bond on the Surface of Au, Ag or Cu
Nature Chemistry 4, 443–455 (2012)
Anal. Chem., 2007, 79 (20), pp 7708–7718
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Polymer capping of Quantum Dots and Carbon Dots
Chem. Mater. 2010, 22, 6361–6369 6361
1. Displace low molecular weight Ligand2. Cross link Dextran on Surface
NOTE:Can also be used to electrostatically adsorb monomers on surface of nanoparticles followed by crosslinking.
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Silane Bonds on Hydroxyl Rich Surfaces
Biomaterials 35(6) · December 2013DOI: 10.1016/j.biomaterials.2013.11.047
NOTE:- Silane bond stability is a function of how densely the –OH groups are spaced on the
target surface- -Sparse –OH groups on non silica surfaces will lead to no oxygen bridges between
silica surface modifers
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Polymer Meshes and Fatty Acid Capping
DOI: 10.1039/C6TB01659F (Paper) J. Mater. Chem. B, 2016, 4, 5418-5428
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Online Resources for Surface Modifying Groups
• Thiols/ Polymers• Sigma-Aldrich (Key Word: Functional thiols)
• Silanes• Gelest (Key word: Orthosilicate)
• Fatty acid• Avanti Polar Lipds
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Outline of the talk• Section I
- Common chemical techniques employed to modify surface of nanoparticles- Thiols, Silanes, polymeric monomers and hybrid schemes
• Section II- Characterization of chemical functional groups on nanoparticles
• Section III- Common conjugation techniques
• Section IV- Published examples of how surfaces are modified to meet end user needs
• Section V- Interesting reads
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1H-NMR to Determine Ratio of Organic Molecules on the Surface of NPs
-CH2-COO-
-CH2-OH
The mole ratio of MUA: MCH: Citrate = 1: 3: 30
Analytical Chemistrydoi:10.1021/ac0709706
Anal.Chem. 79, 7708-7718 (2007)
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FT-IR Characterization of the Surface of NPs
Nanoscale, 2015, 7, 6545-6555
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Outline of the talk• Section I
- Common chemical techniques employed to modify surface of nanoparticles- Thiols, Silanes, polymeric monomers and hybrid schemes
• Section II- Characterization of chemical functional groups on nanoparticles
- NMR and FT-IR
• Section III- Common conjugation techniques
• Section IV- Published examples of how surfaces are modified to meet end user needs
• Section V- An insight into where future functionalization might be headed
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Activating Functional Groups for Further Chemical Modifications
NPs NPs
CH
2CO
O-
NN
NN
CH
2CO
O-
CH
2CO
O-
CH
2CO
O-
+
15 nm
1-15 nmSilica Shell Activated NPs
CH
2CO
O-
NN
NN
CH
2CO
O-
CH
2CO
O-
CH
2CO
O-
NPs Toolbox Passivation Silanization Activation Bioactive
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Common Conjugation Chemistries
• Carbodiimide/Succinimide and NHS esters• Activate carboxylic acid (-COOH) to react with amine (NH3)
• Isothiocyanate• Will react with amines and alcohol in that order of preference
• Maleimide• Reacts with Sulfhydryl groups
• ‘CLICK’ Chemistry• Copper catalyzed• Copper free
Bioconjugation techniqueshttp://www.sciencedirect.com/science/book/9780123822390
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Functional Targets on Biomolecules
• Proteins and Peptides• Amines (-NH3), thiols (-SH), carboxylic acid (-COOH),
Hydroxyl group on glycans (-OH)
• Carbohydrates and glycoconjugates• Hydroxyl groups (-OH) and aldehydes (-CHO)
• DNA/RNA and nucleotides• 5 prime phosphate group or 3 prime –OH group
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Carbodiimide Chemistry: Links –COOH to NH3
Advantages- Cheap- Well characterized reaction- Useful for crosslinking reactions
Disadvantages- Highly susceptible to water hydrolysis- The by product is a toxic urea compound
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Carbodiimide/Succinimide: Links –COOH to NH3
Advantages- Cheap- Well characterized reaction- Useful for protein linking experiment- Amide formation occurs at
Physiological pH
Disadvantages- Highly susceptible to water hydrolysis
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Isothiocyanate [ N=C=S] Reacts with [NH3]Advantages- Well characterized reaction- Useful for fluorescently tagging
protein- Occurs in physiological pH
Disadvantages- Highly susceptible to water
hydrolysis- Also reacts with alcohol
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Reductive Amination of Aldehyde [-CHO]
Advantages- Well characterized reaction- Reacts with amines- Occurs in physiological pH- Suitable for oligonucleotide and carbohydrate conjugations
Disadvantages- Not suited for protein conjugations
https://www.google.com/patents/US6500921
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Maleimide: Reacts with Sulfhydryl (-SH) group
Advantages- Highly stable at physiological pH, up to 3 days- Well characterized reaction- Useful for protein linking experiment- Amide formation occurs at Physiological pH
Disadvantages- If there are disulfide bridges, no reaction will occur- Can inactivate antibodies by disrupting sulfide links between the stem and arm region
http://www.genelink.com/newsite/products/mod_detail.asp?modid=226
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‘CLICK’ Chemistry – Copper Catalyzed
http://medchem101.com/?page_id=142
Advantages- Well characterized reaction- Easy to Store- Amenable to wide range of solvents
Disadvantages- Needs copper as catalysts. Potential toxicity
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‘CLICK Chemistry’- Copper free Strain Promoted
http://medchem101.com/?page_id=142
Advantages- Well characterized reaction- Easy to Store- Amenable to wide range of solvents
Disadvantages- Other than expense none
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‘CLICK Chemistry’- Copper free Thiol-ene Reaction
http://www.advancedsciencenews.com/novel-polymeric-ionic-liquids-through-click-chemistry/
Disadvantages- Thiols have a propensity to form –S-S (disulfide bridges) which reduces their reactivity
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Conjugation Linkers With Spacers
• Homobifunctional Linkers• Cross-linking materials to vary
stiffness and strength
• Heterobifunctional • Introducing functional groups like
streptavidin on surface for further modification
https://www.gbiosciences.com/Protein-Research/Cross-Linking-Modification/Protein-Cross-Linkers/Homobifunctional-Cross-Linkers
http://www.biosyn.com/faq/do-you-offer-azide-or-an-alkyne-modified-dna-sequence.aspx
Dual NHS esters
NHS ester- Azide for Antibody drug conjugates
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Major Types of Bioconjugates• Antibody-enzyme conjugates
• Antibody-drug conjugates
• Fluorescently labeled antibodies
• (Streo)avidin-enzyme conjugates
• Fluorescently-labeled (strept)avidin
• PEGylated druges or proteins
• Anitbody-(nano)particle conjugates for assay or detection
• Immobilized affinity ligands for asay or purification
• Hapten-Carrier Conjugates for vaccines or antibody production
• Fluorescently labeled nucleotides or oligos for next-gen sequencing, PCR or hybridization assays
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How to Create an Optimal Bioconjugate• Design the conjugate with the final application in mind
• Do a small performance test
• Review literature for starting points on reagents, reactions and conditions
• Use solvents that are appropriate for the reagent and reaction conditions
• Reaction conditions that yield maximum active conjugates are important
• Optimize storage conditions to ensure long term stability
• Make several batches using final process to ensure reproducibility
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Outline of the talk• Section I
- Common chemical techniques employed to modify surface of nanoparticles- Thiols, Silanes, polymeric monomers and hybrid schemes
• Section II- Characterization of chemical functional groups on nanoparticles
- NMR and FT-IR
• Section III- Common conjugation techniques
- Carbodiimide, NHS ester, Maleimide, Isothiocyanate, thiolene, Copper free or copper catalyzed CLICK
• Section IV- Published examples of how surfaces are modified to meet end user needs
• Section V- Interesting reads
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Surface Modifications to Match End User Applications
• NOTE: Always account for cross-reactivity when using multiple functional groups
• Stability of functional groups on the surface is always a concern• Simultaneous incorporation of florescence and reactive
functional groups on silica particles
• In situ Synthesis of Functional Groups on the surface of the Nanoparticles
• Biodistribution
• Biosensors
• Immuno Targeting
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A B
C
1. Fluorescent Silica Nanoparticles
Nanoscale, 2013, 5, 10369-10375
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Biocompatibility of Fluorescent Silica Nanoparticles
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2. Problem: Comparing the biodistribution of radiolabeled Silica and radiolabeled Iron Oxide NPs
14C
14C 14C
14C 14C
-COO-
-COO-
-COO--OOC-
-
OOC-
-OOC
Fe3O4-COO-
-COO-
-COO--OOC-
-
OOC-
-OOC
• SiO2 volume labeled with 14C • Surface functionalized with -COO-
• We needed surface similar 14C labeled Fe3O4 NPs to do a comparative study
How do we radiolabel the Fe3O4 NPs without altering the surface functional groups ?
Nanoscale, 2015, 7, 6545-6555
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MgI
MgICO2 + dry ether
COO-
COO-
I. ζ -17.03 ± 2.24 mV
C≡N
C≡N
COO-
COO-
Δ
1N OH-
II. ζ -23.18 ± 1.91 mV
10N OH-, Δ
HCHO + CN-NH
NH2
NH
NH2
CH2COO-
NN
NN
CH2COO-
CH2COO-
CH2COO-
III.
ζ -30.69 ± 2.1 mV
Solution: Synthesize –COO- Functional Groups In Situ with Radiolabeled Precursors
Nanoscale, 2015, 7, 6545-6555
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3. In Vivo Bio-Distribution of 14C Labeled NPs
Dose: 44mg Fe-Si-(14COO-)3/hourTime: 4 hours
CH214COO-
NN
NN
CH2COO-
CH2COO-CH2
14COO-
A B
C DIn Vitro Biocompatibility In Vivo
Nanoscale, 2015, 7, 6545-6555
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The mole ratio of MUA: MCH: Citrate = 1: 3: 30
4. Design of Nanoparticle BioSensor
CIgG
Analytical Chemistrydoi:10.1021/ac0709706
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Imaging and Sensing Single Receptors on Single Living Cells
10 µm
Anal.Chem. 79, 7708-7718 (2007)
Binding kinetics of receptor ligand on cell Surfaces
Binding kinetics of receptor ligand in solution
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4h 8h 24hZ-
Stac
k3D
pro
ject
ion
Anti-C
D133-N
PsA
nti-CD
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SKOV3-IP CD133 (+) cells
5. Immunotargeted Binding to SKOV3-IP Cells
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0.0E+00
1.4E+07
2.8E+07
4h 8h 24h
AuNPs-Anti-CD133 AuNPs-IgG AuNPs
0.0E+00
1.4E+07
2.8E+07
4h 8h 24h
AuNPs-anti-CD133 AuNPs-IgG AuNPs
0.0E+00
1.4E+07
2.8E+07
4h 8h 24h
AuNPs-Anti-CD133 AuNPs-IgG AuNPs
0.0E+00
1.4E+07
2.8E+07
4h 8h 24h
AuNPs-anti-CD133 AuNPs-IgG AuNPs
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Binding Specificity of Immunotargeted Binding to SKOV3-IP Cells
![Page 48: Modulating the Interactions of Nanoparticles with ... · PDF file- Common chemical techniques employed to modify surface of nanoparticles ... streptavidin on surface for further modification](https://reader033.vdocuments.site/reader033/viewer/2022051723/5aaa84ee7f8b9a90188e36c7/html5/thumbnails/48.jpg)
Outline of the talk• Section I
- Common chemical techniques employed to modify surface of nanoparticles- Thiols, Silanes, polymeric monomers and hybrid schemes
• Section II- Characterization of chemical functional groups on nanoparticles
- NMR and FT-IR
• Section III- Common conjugation techniques
- Carbodiimide, NHS ester, Maleimide, Isothiocyanate, thiolene, Copper free or copper catalyzed CLICK
• Section IV- Published examples of how surfaces are modified to meet end user needs
• Section V- Interesting reads
![Page 49: Modulating the Interactions of Nanoparticles with ... · PDF file- Common chemical techniques employed to modify surface of nanoparticles ... streptavidin on surface for further modification](https://reader033.vdocuments.site/reader033/viewer/2022051723/5aaa84ee7f8b9a90188e36c7/html5/thumbnails/49.jpg)
Rapid BioAssays – Environmental Monitoring of E.Coli
Analyst, 2016, 141, 2920
![Page 50: Modulating the Interactions of Nanoparticles with ... · PDF file- Common chemical techniques employed to modify surface of nanoparticles ... streptavidin on surface for further modification](https://reader033.vdocuments.site/reader033/viewer/2022051723/5aaa84ee7f8b9a90188e36c7/html5/thumbnails/50.jpg)
Remote Stem Cell Guidance and Differentiation
DOI: 10.1038/s41467-017-00543-2
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Incorporating Molecular Machines on Surfaces
doi:10.1038/nature23657
![Page 52: Modulating the Interactions of Nanoparticles with ... · PDF file- Common chemical techniques employed to modify surface of nanoparticles ... streptavidin on surface for further modification](https://reader033.vdocuments.site/reader033/viewer/2022051723/5aaa84ee7f8b9a90188e36c7/html5/thumbnails/52.jpg)
Outline of the talk• Section I
- Common chemical techniques employed to modify surface of nanoparticles- Thiols, Silanes, polymeric monomers and hybrid schemes
• Section II- Characterization of chemical functional groups on nanoparticles
- NMR and FT-IR
• Section III- Common conjugation techniques
- Carbodiimide, NHS ester, Maleimide, Isothiocyanate, thiolene, Copper free or copper catalyzed CLICK
• Section IV- Published examples of how surfaces are modified to meet end user needs
• Section V- Interesting reads