moc/bio and nano/micro - university of colorado...
TRANSCRIPT
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Moc/Bio and Nano/MicroLee and Stowell
Biotemplating
Choice of biomaterial
Lipid, protein, nucleic acid
Methods of deposition
Chemical, photochemical
Methods of optimization
Altering the reaction
conditions or the biomaterial
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Remember all the choices…
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Using bio templates for materials
fabrication
Identify a biomaterial with a desired pattern
Determine conditions for materials deposition Chemical or photochemical
Optimize materials formation either chemically or biologically Mutation of biomaterial
Optimization of the chemical process
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Chemical processes
Photopolymerization (primarily organic) Light initiated
Fast and more easily controlled
Clean
Chemical polymerization (primarily organic) Harder to control
Slow
Mineralization (inorganic) Biomineralization of calcium phosphates, magnetite
Slow not well understood or controllable
Electrochemical (metals) Catalysts assisted metal deposition
Somewhat controllable via catalyst doping
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Photochemical polymerization
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Chemical polymerization
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Chemical polymerization
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Electrochemical deposition
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Template mineralization or photochemical polymerization on lipid structures. Archibald, D.D. and S. Mann, Template mineralization of self-assembled anisotropic lipid microstructures. Nature, 1993. 364: p. 430-433. Evans, E., et al., Biomembrane Templates for Nanoscale Conduits and Networks. Science, 1996. 273: p. 933-935.
Bacterial fibres. Davis, S.A., et al., Bacterial templating of ordered macrostructures in silica and silica-surfactant mesophases. Nature, 1997. 385: p. 420-423
Methods for DNA driven nanocrystal organization. Livisatos, A.P., et al.,
Organization of 'nanocrystal molecules' using DNA. Nature, 1996. 382: p. 609-611 ; Chad A. Mirkin, Robert L. Letsinger, Robert C. Mucic & James J. Storhoff DNA-based method for rationally assembling nanoparticles into macroscopic materials Nature 1996, 382, p 607
S-layer templates for nanoparticle fabrication. Sleytr, U. B. et al. Applications of
S-layers. FEMS Microbiol Rev 20, 151-75 (1997).
Two-dimensional array fabrication using ferritin. Yamashita, I., fabrication of two-dimensional array of nano-particles using ferritin molecule. Thin solid films, 2001. 393: p. 12-18.
Chemoselective Templates from TMV. 1. Demir, M. & Stowell, M. H. B. A chemoselective biomolecular template for assembling diverse nanotubular materials. Nanotechnology 13, 541-544. (2002). Biotemplate Synthesis of 3-nm Nickel and Cobalt Nanowires Mato Knez et al Nanoletters 2003 Vol. 3, No. 8 1079-1082
Bacterial Phage virus. Mao, C. et al. Virus-based toolkit for the directed synthesis of magnetic and semiconducting nanowires. Science 303, 213-7 (2004).
3D DNA templates. Shih, W. M., Quispe, J. D. & Joyce, G. F. A 1.7-kilobase single-stranded DNA that folds into a nanoscale octahedron. Nature 427, 618-21 (2004).
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Lipid templates
Recall lipids and their
many phases
Cubic,hexagonal,etc
Use stable lipid phases
as a template
Polymerization or
mineralization
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Cerebrosides (tube forming)
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Iron oxide deposition
Various forms of Fe oxide were produced
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Bacterial Fibres
What are they
Like drawn nylon from
polymerizing solutions
How to use them as a
template
Actually using the
interstices between the
bacterium
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Silicate formation at the
interstices
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After pyrolysis to remove
organic matter
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2D-DNA templates
Linear arrays can be
made using gold
particles
2D arrays possible
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Control of size and spacing
Distance
Siz
e
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Bacterial S-layers
Outer surface protein layer of bacteria
Highly regular ordered 2D array
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Mineralization using CdS
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Can also use to pattern and
assemble other protein layers
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Lithographic methods for nano
to micro scale patterning
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Ferritin and other spherical
cages for nanodot formation
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http://www.ncbi.nlm.nih.gov/ICTVdb/
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Making a 2D array of
nanoparticles using lipids
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Single Nanodot arrays
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Array of 5nm quantum dots from
ferritin
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Designing a self assembled single
electron transistor (SASET)
7nm gate and 4 nm interconnects
WiresGates Self Assembly
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Designing a self assembled single
electron transistor (SASET)
Nanodots
Nanowires
Self assembly
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Ferritin
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Biomineralization
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TEM following mineralization
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Designing a self assembled single electron
transistor (SASET)
Nanodots
Nanowires
Self assembly
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Strategy for wires
Prototypical tobamovirus
18nm diameter 300nm length
~2100 identical protein monomers
17.5 kD coat protein monomer
Very stable to a variety of conditions
Genetically accessible
Structure of monomer available
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Genetic modification TMV to
achieve a chemoselective
template.
The three potential external labeling positions
N-terminus
C-terminus
63-66 loop
Conversion of Threonine to Lysine
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N-Hydroxy-Succinamide
Facile
Specific primary amine reactive
Inexpensive
Mild and biocompatible products
Variety of solvent conditions
Aqueous or non-aqueous
O
O
N
O
OL
RNH2
O
N
O
OH
O
L NHR
>99%
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C-term
N-term
63-66 loop
Rational for exterior labeling
ssRNA
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Mutagenesis and expression
•Subcloned coat coding region
•PCR mutagenesis
•Re-ligate back mutated fragment
•Transcription and plant dusting
TMV 9kB
pUC19
2.6 kBPCR mutagenesis
mTMV 9kBX
[ ] Excise and religate
pUC19
TMV 6kB
pUC19
mTMV 6kB
Transcription
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Quantification of labeling
•Quantitative labeling of mutant TMV (mTMV) 106+/- 12%
•No labeling of native TMV (nTMV)
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10x
Fluorescent labeling
EM
Confocal
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Ordered assembly of avidin on biotin TMV
Biotin labeled
+Avidin+Avidin
+Avidin
Computed diffraction
pattern from avidin treated
biotin labeled TMV
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Metal coating via in situ photoreduction
Cu++
e-
hv
O
OH OH
O
OH
OH
Cu+
e-
Cu
O
OO
O
OH
OH
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Metal coated TMV
+hn-hn
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Tobacco Mosaic Virus
Prototypical tobamovirus
18nm diameter 300nm length
~2100 identical protein monomers
17.5 kD coat protein monomer
Very stable to a variety of conditions
Genetically accessible
Structure of monomer available
Internal diameter ~4nm
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Can we apply a similar strategy for
labeling the interior of TMV (4nm)
ssRNA
2 internal Threonines
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Repeat the similar mutation
strategy for internal Threonines
Mass difference 27.1
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Confirmed by MALDI-TOF
Native expect 17529
Mutant expect 17566
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3nm wires on the inside of
TMV
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Designing a self assembled single
electron transistor (SASET)
Nanodots
Nanowires
Self assembly
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Strategy
Use SELEX to determine RNAs with high
affinity to Ferritin
Introduce such sequences into TMV RNA
Test directed assembly
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SELEX Systematic Evolution of Ligands by
EXponential enrichment” (Tuerk and Gold, 1990)
10 random positions and 4 possible bases 410 or > 106 different sequences
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Panning and selection
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Assembly of virus
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Use of ferritin symmetry sites
(432)
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Bacterial phages
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Mutations based on peptides
selected to bind materials
Insert peptide sequence
into phage gene
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Phage M13
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Nanowires after annealing
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3D-DNA templates.
Chen, J. H. & Seeman,N. C. Synthesis from DNAof a molecule with
the connectivity of a cube. Nature 350, 631–633 (1991).
Shih, W. M., Quispe, J. D. & Joyce, G. F. A 1.7-kilobase single-stranded
DNA that folds into a nanoscale octahedron. Nature 427, 618-21
(2004).{Shih, 2004 #849}
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DNA surface nanostructures
Lui et al Nanolet 2002, Vol 2, 863
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Virus templates for Gold Cell
Sensors
Introduce gold particles in to viruses
Infect cells with the virus and perform raman
imaging of the cell
~30nm resolution
Image cellular conditions
pH
Metabolites
etc
http://www.indiana.edu/~bdlab/
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Addition of gold particles
Dissociate viral shell proteins at low pH and then
reassemble in the presence of 5 nm gold particles
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High resolution IR imaging of
cell interior
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Chaperonins as nanodot
depots
Ishi et al, Nature 2003, Vol 423, 628
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Some dimensions
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Uptake of CdS particles
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Demonstration of complete
release of the CdS particles
Size exclusion chromatography, UV:protein F:CdS.
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EM analysis in absence and
presence of ATP
CdS qdot
With ATPWithout ATP
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A piconewton force meter
Hess et al , Nanolet, 2002, Vol 2 , 1113.
Tool for measuring pN
to nN forces.
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•Biological systems offer a direct route into highly ordered well
defined templates for nanomolecular assembly.
•Many such systems can be genetically altered to optimize a
variety of desired properties
•Can be used for nanoscale pattering and enhanced lithography
•Such system open the way for highly efficient, inexpensive self
assembled systems for materials fabrication at the
nanoscale.
Conclusions