mlab 2434 – microbiology keri brophy-martinez
DESCRIPTION
MLAB 2434 – MICROBIOLOGY KERI BROPHY-MARTINEZ. Use of Colony Morphology for Presumptive ID of Microorganism. Importance of Colonial Morphology as a Diagnostic Tool. Provide a presumptive diagnosis in times of critical need - PowerPoint PPT PresentationTRANSCRIPT
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MLAB 2434 – MICROBIOLOGYKERI BROPHY-MARTINEZ
Use of Colony Morphology for Presumptive ID of Microorganism
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Importance of Colonial Morphology as a Diagnostic Tool
Provide a presumptive diagnosis in times of critical need Best guess giving type of specimen and what is seen
before confirmatory tests Help jump-start therapy
Enhance quality of patient care through rapid results and cost effectiveness Prevents time-consuming testing that yields few
results Play a significant role in quality control
Helps maintain accuracy of commercial or automated systems
Troubleshoot mixed cultures or errors in commercial systems
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Colony Morphology (cont’d)
Initial Observation & Interpretation of Cultures“Read” routine cultures after 18
to 24 hours of incubation If cultures cannot be read after
24 hours, refrigerate to prevent overgrowth and drying
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Colony Morphology (cont’d)
Compare growth on different media
Examples:• If good growth on BAP but no growth
on MAC, the bacteria is probably a gram positive organism
• If MAC growth is pink, the organism is a lactose fermenter
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Fermenting vs. Non-Fermenting
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Colony Morphology (cont’d)
Gross Colony CharacteristicsHemolysis on BAP (Chocolate
agar does not show true hemolysis)• α hemolysis
• green discoloration on the media• Due to partial lysing of the RBC’s
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Colony Morphology (cont’d)
β hemolysis – complete clearing of blood around colonies on BAP
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Colony Morphology (cont’d)
Gamma hemolysis Absence of
hemolysis, nonhemolytic
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Colony Morphology (cont’d)
Size ( large, medium, small, pinpoint)
Margin or edge (smooth, filamentous)
Elevation ( raised, convex, flat)Density( transparent, translucent,
opaque)Consistency (brittle, creamy, dry)Pigment (production of color)Odor (fruity, old sock)
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Colony Morphology (cont’d)
Shape, edge and elevation of bacterial colonies
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Liquid Media Environment
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Growth of Organisms in Liquid Media
Streamers or vines are associated with certain streps
Puff balls associated with streps
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Growth of Organisms in Liquid Media Turbidity
Cloudiness of the medium
Often see gas
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References
Engelkirk, P., & Duben-Engelkirk, J. (2008). Laboratory Diagnosis of Infectious Diseases: Essentials of Diagnostic Microbiology . Baltimore, MD: Lippincott Williams and Wilkins.
Mahon, C. R., Lehman, D. C., & Manuselis, G. (2011). Textbook of Diagnostic Microbiology (4th ed.). Maryland Heights, MO: Saunders.