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F A C U L T Y O F S C I E N C E U N I V E R S I T Y O F C O P E N H A G E N
Mixotrophic Protists among Marine Ciliates and Dinoflagellates: Distribution, Physiology and Ecology
Academic advisor: Associate Professor Per Juel Hansen
Submitted: 29/04/10
PhD thesis Woraporn Tarangkoon
Contents
List of publications 3
Preface 4
Summary 6
Sammenfating (Danish summary) 8
สรุป (Thai summary) 10
The sections and objectives of the thesis 12
Introduction 14 1) Mixotrophy among marine planktonic protists 14
1.1) The role of light, food concentration and nutrients for 17
the growth of marine mixotrophic planktonic protists
1.2) Importance of marine mixotrophic protists in the 20
planktonic food web
2) Marine symbiont-bearing dinoflagellates 24
2.1) Occurrence of symbionts in the order Dinophysiales 24
2.2) The spatial distribution of symbiont-bearing dinoflagellates in 27
marine waters
2.3) The role of symbionts and phagotrophy in dinoflagellates with symbionts 28
3) Symbiosis and mixotrophy in the marine ciliate genus Mesodinium 30
3.1) Occurrence of symbiosis in Mesodinium spp. 30
3.2) The distribution of marine Mesodinium spp. 30
3.3) The role of symbionts and phagotrophy in marine Mesodinium rubrum 33
and Mesodinium pulex
Conclusion and future perspectives 36
References 38
Paper I
Paper II
Paper III
Appendix-Paper IV
Appendix-I
3
Lists of publications
The thesis consists of the following papers, referred to in the synthesis by their roman
numerals. Co-author statements are attached to the thesis (Appendix-I).
Paper I
Tarangkoon W, Hansen G Hansen PJ (2010) Spatial distribution of symbiont-bearing
dinoflagellates in the Indian Ocean in relation to oceanographic regimes. Aquat Microb Ecol
58:197-213.
Paper II
Tarangkoon W, Hansen PJ (Submitted) Prey selection, ingestion and growth responses of the
common marine ciliate Mesodinium pulex in the light and in the dark.
Aquat Microb Ecol
Paper III
Hansen PJ, Moldrup M, Tarangkoon W, Garcia-Cuetos L, Moestrup Ø (draft manuscript)
Does the marine red tide ciliate Mesodinium rubrum have replaceable symbionts?
Appendix-Paper IV
Farnelid H, Riemann L, Tarangkoon W, Hansen G, Hansen PJ (Submitted)
Putative N2-fixing heterotrophic bacteria associated with dinoflagellate-cyanobacteria
consortia in the low-nitrogen Indian Ocean Aquat Microb Ecol
Paper I is reprinted with kind permission from Inter-Research
4
Preface
This thesis is written as part of the fulfillment of PhD degree from the Faculty of Science,
University of Copenhagen. My PhD grant was supported from Rajamangala University
Srivijaya, Thailand.During the PhD period, I was based at the Marine Biological Laboratory
(MBL), Helsingør. However, Paper I & Appendix-paper IV were based on results from
materials sampled during the “Galathea 3” expedition (Leg 7).
Heartfelt thanks to my supervisor, Per Juel Hansen, for all his help and kindness
throughout my study. I could not have done it without him. Apart from him being a superb
supervisor in an academic way (to open my eyes on culture experiments, contribute ecological
thinking, guide the writing etc.), he has also solved my many life abroad obstacles during my
study (language, finding a place to stay, visa problem, etc). He is always patient, always
stimulate me during my difficult moments, and a companion along the way. “Thank you for
being you, Per”. I am also grateful for the stimulating contribution of the other co-authors in
my 4 papers. It has been a great pleasure to collaborate with you. I am particularly grateful to
Torkel G. Nielsen for providing me with the opportunity to join Leg 7 in the “Galathea 3”
expedition and sharing environmental data. I also thank other scientists on board, especially
Thomas Kiørboe, Andre Visser, Karen Marie Hillingsø, Maria Hastrup Jensen, Carsten Smidt
(Captain of HMDS ‘Vædderen’) and his crew for their help during Leg7. Sincere gratitude to
Michael Olesen, regardless of what questions I brought up to him e.g. Danes life, religion,
relationship, He was always open for an enthusiastic discussion.
I am grateful to all members of the plankton group at the MBL, particularly Lasse T.
Nielsen, Karen Riisgaard, Louise K. Poulsen, and Morten Moldrup, who always tried to
understand my Thai-English accent and were helpful. Special thanks to Morten, who was
frequently bothered (questioning, asking for help, etc) by me during all these years. All my
officemates, Jane W. Berens, Herik Staahl, Jon Svendsen, Michael van Deurs, Maria F.
Steinhausen, Bjørn Tirsgård are thanked for their help and friendly atmosphere. Another
special thank is given to Marianne Ernsted, for her help with finding books and big warm
hugs when I was frustrated and homesick. Birgit Thorell Lyck, Marriane Saietz, and other
staffs at the MBL and the Aquarium also deserve my thanks for their support and help during
my study.
5
Many thanks to Marc Staal and Carola Simon for your generous and all
thoughtfulness, you guys are my Dutch big brother and sister who have taken good care of
me, in my academic as well as during free time through these years. Cátia Carreirra, Christian
Lønborg, Alejandro M. Garcia, are thanked for kindly help, making me laugh and having
good times even though we have only met at the last phase of my stay in Denmark but your
friendship and kindness are memorized. I am also lucky that I met my new Thai friends and
their in laws, Worasiri&Urlik Pederson, Akkaraya&Allan Nielsen, Chanchira&Dennis
Mølbæk, Atchaneey Chamnansinp. Thanks for your warm hospitality and help.
I am deeply grateful to my colleagues at the Marine Science Department, Faculty of
Science and Fisheries Technology, Rajamangala University Srivijaya for their support and
hard work while I was away. Thanks are specially given to Suwat Tanyaros, for the achieved
grant and encouragement, and to Pornthep Wiruchawong for listening to all my problems and
being helpful in a general sense. Suree Satapoomin and Ajcharaporn Piumsomboon are
appreciated for their help, advice and support. Without their help I might not end up having
my PhD in Denmark. I am indebted to Suriyan Saramul for his advice in using Surfer
program, Patama Singhruck, Itchika Sivaipram for always being there, listening and
understanding all my issues.
Last but not least, million thanks are not enough for my beloved family, mom, dad, my
sister, my brother, and my grandparents. Without their love, support and beliefs in me, I could
never have been strong enough to endure the cold and dark winters in Denmark and finish my
PhD.
28th of April 2010
Woraporn (Mam)
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Summary
The mixotrophic protists (= protists which combine heterotrophy and photosynthesis) are
common in marine waters around the world. They vary widely in their photosynthetic and
ingestion capabilities and they add a further complication to the marine planktonic food web.
This thesis focused on 2 groups of mixotrophic protists: 1) symbiont-bearing dinoflagellates
and 2) ciliates belonging to the genus Mesodinium spp.
The spatial distribution (horizontal and vertical) of symbiont-bearing dinoflagellates
(order Dinophysiales, genera Ornithocercus, Histioneis Parahistioneis, Cithoristes,
Amphisolenia, Triposolenia) was investigated along a transect from the deep ocean (Indian
Ocean) to shallow coastal waters (North West off Australia), as well as on a transect outside
Broome (Australia). The symbionts of these dinoflagellates are either prokaryotic (e.g.
heterotrophic bacteria, cyanobacteria) or eukaryotic algae. Cell concentrations of these
dinoflagellates were very low in these waters (< 4 cells L-1). The ectosymbionts-bearing
dinoflagellates were most common and had the highest species diversity in waters
characterized by high temperatures (> 28 ºC) and very low nitrogen concentrations (< 0.4µM).
Using light and transmission electron microscopy, we could demonstrate that Ornithocercus
spp. ingested not only their ectosymbionts but also other prey items (i.e. ciliates). For future
research on their physiology, the successful establishment of these organisms in laboratory
culture is required.
The ciliate genus Mesodinium contains heterotrophic and mixotrophic species (so far
only one symbiont containing species M. rubrum has been described). This study investigated
the prey selection, photo and feeding physiology of a non-symbiont containing Mesodinium
species, M. pulex. The results showed that Mesodinium pulex ingests a variety of prey cells,
but that ingestion rates and especially growth rates varied depending upon the diet. The effects
of light and prey concentration on photosynthesis, ingestion and growth rate of M. pulex was
studied in detail when fed the dinoflagellate Heterocapsa rotundata, The photosynthetic
performance of Mesodinium pulex was quite small, amounting for less then 4 % of its carbon
uptake, indicating that M. pulex is primarily a heterotrophic species. Despite this, light
affected ingestion rates. Ingestion rates increased by a factor of 2 in the light compared to in
the dark. Consequently, growth rates also increased in the light.
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The ciliate Mesodinium rubrum contains symbionts of cryptophyte origin. In the
laboratory, our strain of Mesodinium rubrum is normally cultured on cryptophytes within the
“Teleaulax clade”. Prey selection of M. rubrum was investigated by offering different prey
types (i.e. cryptophytes, dinoflagellate). Mesodinium rubrum ingested all the offered prey, but
it could only maintain sustained growth when fed on Teleaulax amphioxeia. To test whether
the symbionts of M. rubrum are permanent or temporary (replaceable), M. rubrum cultures
were offered preys from 4 different cryptophyte clades. TEM pictures of M. rubrum revealed
no evidence of sequestered chloroplasts from the prey. Also, the molecular data could not
confirm that chloroplasts of M. rubrum can be replaced when offered other cryptophyte prey
outer the “Teleaulax clade”.
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Sammenfating Mixotrofe protister (dvs. protister, der kombinerer en heterotrof og autotrof levevis) er yderst
almindelige i verdenshavene. De varierer utroligt meget i deres evne til at fotosyntetisere og
optage føde. Dette komplicerer unægtelig det planktoniske fødenet yderligere. Denne
afhandling har fokuseret på 2 grupper af mixotrofe organismer: 1) symbiontbærende
dinoflagellater og 2) ciliater tilhørende slægten Mesodinium spp.
Den rummelige fordeling (horisontalt og vertikalt i vandsøjlen) af de
symbiontbærende dinoflagellater (i ordenen Dinophysiales mere specifikt slægterne
Ornithocercus, Histioneis Parahistioneis, Cithoristes, Amphisolenia, Triposolenia) blev
undersøgt langs et transekt gående fra det dybe Indiske Ocean til grunde kystnære vande ved
Nordvest Australien. Desuden undersøgtes et transekt udenfor Broome (Australien).
Symbionterne hos disse dinoflagellater er enten prokaryoter (dvs. heterotrofe bakterier eller
cyanobakterier) eller eukaryote alger. Cellekoncentrationerne af disse dinoflagellater var
meget lave i disse vande (< 4 celler L-1). Blandt disse var de ectosymbiontbærende
dinoflagellater de mest almindelige, og vi fandt den største artsdiversitet i vande med høj
temperatur (> 28 ºC) og lave nitrogen koncentrationer (< 0.4µM). Ved hjælp af lys og
transmissions elektronmikroskopi viste vi at Ornithocercus spp. udover at æde deres deres
ectosymbionter, også ernærede sig af andre fødeemner (f. eks. ciliater). Fremtidige studier af
disse dinoflagellaters fysiologi vil være dybt afhængige af at kunne etablere
laboratoriekulturer.
Ciliatslægten Mesodinium indeholder heterotrofe samt mixotrofe arter (endnu er der
kun beskrevet en enkelt symbiontbærende art). Dette studie undersøgte bytteselektion samt
foto- og fødeoptagelses-fysiologi hos en Mesodinium-art uden symbionter, Mesodinium pulex.
Her kunne vi vise, at M. pulex optager et bredt udvalg af arter, men at fødeoptagelsesrater, og
især vækstrater varierer alt efter byttet. Effekten af lys og fødekoncentration blev undersøgt i
detaljer, når M. pulex blev tilbudt dinoflagellaten Heterocapsa rotundata som føde. Den målte
fotosyntese var ret lille, og udgjorde mindre end 4 % af det totale kulstofoptag. Heraf følger at
M. pulex formodentligt primært er en heterotrof art. Til trods for dette, så vi at lys påvirkede
fødeoptagelsesraten, og denne fordobledes i lys i forhold til mørke. Dette påvirker så igen
vækstraten, således at denne er højere i lys end i mørke.
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Ciliaten Mesodinium rubrum indeholder en symbiont af rekylalge (crytophycé)
oprindelse. For at undersøge om symbionten er permanent eller midlertidig, undersøgtes
fødeselektion også her. Mesodinium rubrum blev tilbudt et bredt udvalg af føde i passende
størrelse (crytophycéer og dinoflagellater). Alle byttetyper blev optaget af Mesodinium
rubrum, men vedvarende vækst sås kun når den blev tilbudt Teleaulax amphioxeia, som
tilhører ”Teleaulax kladen”. Så for at teste hvorvidt symbionten var permanent eller
midlertidig (dvs. udskiftelig), blev den tilbudt cryptophycéer fra 4 forskellige klader. TEM
billeder kunne ikke vise at Mesodinium rubrum havde tilegnet sig kloroplastre fra sit bytte.
Heller ikke molekylære metoder kunne vise udskiftning af symbionter, hvis ciliaten fik tilbudt
byttealger uden for ”Teleaulax kladen”.
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สรุป (Thai Summary)
โปรโตซัวท่ีดํารงชีพแบบ Mixotroph (=โปรโตซัวท่ีดํารงชีพแบบผสมโดยกินอินทรียสารและสังเคราะหแสงแบบพืช) เปนกลุมท่ีพบไดท่ัวไปในทะเลท่ัวโลก ความสามารถที่หลากหลายในการสังเคราะหแสงและการกินอาหารของพวกมันทําใหสายใยอาหารในทะเลซับซอนย่ิงขึ้น วิทยานิพนธฉบับน้ีมุงเนนโปรโตซัว Mixotroph 2 กลุม คือ ไดโนแฟลกเจลเลตท่ีมี symbionts อาศัยอยูรวม และซิลิเอตในสกุล Mesodinium การศึกษาคร้ังน้ีศึกษาการแพรกระจายตามพื้นท่ีในแนวนอนและแนวด่ิงของไดโนแฟลกเจลเลตท่ีมี symbionts อาศัยอยูรวม ไดแก ไดโนแฟลกเจลเลตในวงศ Dinophysuales เฉพาะสกุล Ornithocercus, Histioneis Parahistioneis, Cithoristes, Amphisolenia, Triposoleniaจากบริเวณนํ้าลึกมหาสมุทรอินเดียไปยังแนวชายฝงท่ีตืน้ฝงตะวันตกเฉียงเหนือของประเทศออสเตรเลีย และรวมถึงบริเวณนอกฝงเมือง Broome ประเทศออสเตรเลีย พบวาประเภทของ symbionts ในไดโนแฟลกเจลเลต คือ prokaryotic (ตัวอยางเชน แบคทีเรีย ไซยาโนแคทีเรีย) หรือ สาหรายเซลลเดียว ซ่ึงพบไดโนแฟลกเจลเลตท่ีมี symbiont อาศัยอยูรวมในความหนาแนนต่ํา (นอยกวา 4 เซลลตอลิตร) ตลอดพ้ืนท่ีศึกษา นอกจากน้ันไดโนแฟลกเจลเลตที่มี ectosymbionts (symbiontsท่ีอาศัยอยูดานนอกเซลล) สามารถพบไดท่ัวไปและมีความหลากชนิดสูงในนํ้าท่ีมีอุณหภูมิสูง (สูงกวา 28 ºC ) และสารอาหารไนโตรเจนตํ่า (ต่ํากวา 0.4μM) การศึกษาองคประกอบภายในเซลลของไดโนแฟลกเจลเลตสกุล Ornithocercus แสดงวาไดโนแฟลกเจลเลตสกุลน้ีสามารถกินอาหารท่ีเปนท้ัง symbionts ของพวกมันเองและส่ิงมีชีวติอ่ืน เชน ซิลิเอต ท้ังน้ีการศึกษาวจิัยเก่ียวกับสรีรวิทยาของไดโน-แฟลกเจลเลตที่มี symbionts อาศัยอยูรวมในอนาคตข้ึนอยูกับความสําเร็จในการเล้ียงไดโนแฟลกเจลเลตกลุมน้ีในหองปฏิบัติการ ซิลิเอตในสกุล Mesodinium ประกอบดวยชนิดท่ีดํารงชีพแบบกินอินทรียสารซ่ึงพบไดหลากกลายชนิดและชนิดท่ีดํารงชีพแบบกินอินทรียและสังเคราะหแสงแบบพืชผสมกัน ซ่ึงในปจจุบันพบเพียงชนิดเดียวคือ Mesodinium rubrum ท่ีมี symbionts อยูดวย การศึกษาคร้ังน้ีมุงศึกษาการเลือกสรรเหย่ือ การสังเคราะหแสงและสรีรวทิยาการกินอาหารของ Mesodinium อีกชนิดหน่ึง คือ Mesodinium pulex ซ่ึงเปนชนิดท่ีไมมี symbiont อาศัยอยูรวม วาสามารถดํารงชีพแบบ mixotroph ไดหรือไม ผลการศึกษาพบวา M. pulexสามารถกินเหย่ือไดหลากชนิดแตอัตราการกินและอัตราการเติบโตของ Mesodinium pulex ผันแปรตามชนิดของเหย่ือ การศึกษาอิทธิพลของความเขมแสงและปริมาณเหย่ือวามีผลตออัตราการสังเคราะหแสง อัตราการกินและการเติบโตของ M. pulex เม่ือใหไดโนแฟลกเจลเลตชนิด Heterocapsa rotundata เปนเหย่ือ พบวาอัตราการสังเคราะหแสงต่ํากวา 4% ของคารบอนจากการกิน ซ่ึงบงชี้วา M. pulex มีการดํารงชีพแบบกินอินทรียสาร
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เปนหลัก นอกจากน้ีแสงมีอิทธิพลตออัตราการกินโดยเพ่ิมอัตราการกินขึ้นเปน 2 เทาในท่ีมีแสงพอเพียงเมื่อเทียบกับท่ีมืดสนิท ซ่ึงสงผลใหการเติบโตในท่ีมีแสงสูงขึ้นดวยเชนกัน ซิลิเอตอีกชนิดในสกุลเดียวกันน้ี Mesodinium rubrum ซ่ึงเปนซิลิเอตท่ีมี symbionts ชนิดท่ีตนกําเนิดเปนสาหราย cryptophyte การศึกษาเร่ืองการเลือกสรรเหย่ือของ M. rubrum สายพันธุเดนมารก ท่ีเล้ียงดวยสาหราย cryptophyte ในกลุม “Teleaulax clade” ในหองปฏิบัติการ โดยใหเหย่ือหลายชนิด (ตัวอยางเชน ไดโนแฟลกเจลเลต สาหราย cryptophyte) พบวาซิลิเอตชนิดน้ีกินเหย่ือทุกชนิด แตมีการเติบโตเม่ือไดรับอาหารชนิด Teleaulax amphioxeia เพียงชนิดเดียว ศึกษาวา symbionts น้ันเปนแบบอาศัยรวมแบบถาวรหรือเปล่ียนแทนท่ีไดในซิลิเอต M. rubrum โดยการใหเหย่ือเปนสาหราย cryptophyte ตางชนิดจาก 4 กลุม (clade) ท่ีตางกัน สัณฐานวิทยาภายในของเซลลซิลิเอตเมื่อกินสาหราย cryptophyte ไมพบหลักฐานวา chloroplasts ของเหย่ือถูกเก็บรักษาไวโดยซิลิเอต และผลการศึกษาทางชวีโมเลกุลยืนยันวา chloroplasts ของซิลิเอต M. rubrum น้ันไมสามารถเปล่ียนแทนท่ีโดยสาหราย cryptophyte นอกกลุม Teleaulax clade ได
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The sections and objectives of the thesis
Mixotrophy among protists is widespread in marine waters, especially among ciliates and
dinoflagellates. Some types of mixotrophic protists have not yet been established in laboratory
culture, mainly because our knowledge of their feeding preferences and physiology is very
restricted. In those cases, important information on their biology can be gathered from
studying their distribution in the field in relation to environmental factors like light, inorganic
nutrients, temperature etc. Other types of mixotrophic protists have recently been brought into
culture, but our knowledge of their physiology and prey preferences is in many cases still very
limited.
The first part of this thesis focuses on how the distribution of the symbiont-bearing
dinoflagellates (Order Dinophysiales) is related to environmental factors in the Indian Ocean.
None of these species have ever been established in laboratory culture and very little is known
about their distribution and biology. This study was part of larger project carried out during
the “leg 7” of the Galathea 3 expedition, autumn 2006. The objective of this work was to 1)
study the spatial distribution (vertical and horizontal distribution) of the symbiont-bearing
dinoflagellates from oceanic stations to coastal stations during the cruise from Cape Town,
South of Africa to North West Australia, 2) relate the cell distributions to physical and
chemical variables, 3) study the relationship between the symbionts and the host cells. This
includes the use of light and electron microscopy to reveal the contents of food vacuoles
(Paper I). Finally, I collected a large number of species during the cruise in order to study the
potential of these symbionts to fix N2 (i.e. having NifH genes). Since the molecular work,
which is the bulk part of the work, was carried by colleagues at the Linnaeus University and I
therefore just attached this part of my work as an appendix (Appendix-Paper IV).
The second part of my thesis deals with the ciliates Mesodinium spp, which are
common and important species in marine waters. This part aimed at understanding the prey
selection and feeding physiology of a non-symbiont containing Mesodinium pulex and the
symbiont containing species M. rubrum (Paper II, Paper III). I specifically investigated the
photosynthetic rate of M. pulex cells to test to what extent this species rely on photosynthesis
for growth and survival (Paper II). I also studied the effects of light on ingestion and growth
rates of M. pulex. There is an ongoing discussion on whether the symbionts of M. rubrum are
13
replaceable or not. Thus, I studied this by offering M. rubrum different types of cryptophytes
and monitored the growth and ingestion rates of M. rubrum in each case. The fate of ingested
prey cells were investigated using a combination of ultrastructural and molecular techniques
(Paper III).
Introduction
14
Introduction This introduction comprises of 3 parts of which the first part presents the current knowledge
of marine mixotrophic protists in general. The second and third parts of the introduction
present what we do know on the specific groups of marine mixotrophic protists (i.e. symbiont-
bearing dinoflagellates and Mesodinium spp.), which I have studied during my PhD study.
Part 1. Mixotrophy among marine planktonic protists
Mixotrophy is a term often used to describe the use of mixed nutrition by an organism. The
most popular use of this term refers to the combined use of photosynthetic and heterotrophic
nutrition (e.g. phagotrophy, osmotrophy, saprotrophy) in a single organism (Caron 2000,
Stoecker 1999, Jones 1994, Burkholder et al. 2008).
Mixotrophic planktonic protists are common in aquatic habitats around the world.
They can be found in polar to equatorial regions, in coastal to oceanic waters, in freshwater as
well as in seawater; they can even be encountered in super euryhaline waters. Also, they live
in nutrient poor as well as in eutrophic waters (reviewed in Stoecker 1999, Burkholder et al.
2008). Mixotrophic protists vary widely in their photosynthetic and ingestion capabilities and
may be considered to occupy a wide spectrum of nutritional strategies from absolute
autotrophy to absolute heterotrophy (Jones 1994). Therefore, mixotrophic planktonic protists
complicate the flow of energy and nutrients in food webs by functioning both as producers
and consumers, rendering classical models of aquatic ecosystems incomplete (Fig. 1). For
example, mixotrophic planktonic protists compete with phototrophs and bacteria for dissolved
inorganinc and organic nutrients and prey on them at the same time (Ptacnik et al. 2004).
Mixotrophic protists may not only compete with phototrophs for soluble nutrients, but may in
some cases even actually facilitate them (Rothhaupt 1996). Mixotrophic protists prey on a
variety species and sizes of prey depending upon their feeding habits. Thus, different feeding
strategies may put them on different trophic levels in planktonic food webs (Hansen & Calado
1999).
Introduction
15
Fig. 1 Marine mixotrophic protists in the marine microbial food web. Solid arrows represent pathways of consumption of organic matter; dashed arrows represent pathways by which organic matter, both dissolved (DOM) and particulate (POM) is released from living organisms (adapt from Caron 2000).
Roger I. Jones (Jones 1994) was the first to describe mixotrophy nutrition as a
spectrum of nutritional strategies from absolute heterotrophy over mixotrophy to absolute
autotrophy in aquatic protists (Fig. 2). He separated the organisms into three groups. The first
group comprises of organisms which have chloroplasts of their own (left side on Fig. 2). In
this group the entire mixotrophic continuum can be found from largely phototrophic to large
heterotrophic organisms. The second group is made up of organisms which can sequester
(steal) functional chloroplasts from algal prey (right side bottom). These organisms are
considered mainly heterotrophic organisms which mainly use photosynthesis to survive
periods of starvation. The third group comprises of organisms which form symbiosis with
alga. These are considered mainly to belong to the phototrophic end of the spectrum.
DOM & POM
Phytoplankton
Herbivorous protists
Mixotrophs protists
Bacterivorous protists
Heterotrophic bacteria
Viral lysis
Zooplankton
Introduction
16
Fig. 2 Schematic representation of the continuum of nutritional strategies amongst planktonic
protists. Examples are shown of genera which include mixotrophic forms amongst their species.
(Jones 1994)
Harriet JL Jones (Jones 1997) further discussed the role of light and prey concentration
for aquatic (but mainly freshwater) mixotrophs with their own chloroplasts (= mixotrophy in
phytoflagellates). She categorized these phytoflagellates into 4 groups of mixotrophics (A, B,
C and D) depending upon the respective role of photosynthesis and phagotrophy: Group A
comprises of organisms that are primarily heterotrophic and phototrophy is employed only
when prey concentrations limit heterotrophic growth. In all the other 3 groups (B, C and D)
phototrophy is the dominant mode of nutrition. In group B, phagotrophy supplements growth
when light is limiting, therefore growth is inversely proportional to light intensity. In group C,
phagotrophy provides essential substances for growth and ingestion is proportional to light
intensity. Group D includes protists which have very low ingestion rates, ingesting prey only,
for example, for cell maintenance during prolong dark periods. Later, Stoecker (1998) made a
review in which the goal was to include all types of mixotrophy, thus including the protists
with symbionts and those which sequester chloroplasts from their prey. In this review she
treated, besides light and prey concentration, also the role of nutrients and growth factors. She
Introduction
17
came up with 3 main types for which she made predictions for their dependency on light, prey
concentration and nutrients. In short, Type I are the ‘ideal mixotrophs’ which are able to
utilize phototrophy and phagotrophy equally well. The type II, covers all what she called the
primarily phototrophic “algae”, which for different reasons supplements photosynthesis with
food uptake. Finally, her type III mixotroph, covers predominantly heterotrophic protozoa
which use photosynthesis for survival in the light when food is scarce or use photosynthesis as
a supplement in carbon metabolism. The three reviews mentioned above (Jones 1994, Jones
1997, Stoecker 1999) are all more than 10 years old and were all build upon a very limited
number protist species of which many species were freshwater species. Since then many more
marine protists have been studied.
In the following part (part 1), I will try in a short way to summarize our current
knowledge on role of light, prey concentration and nutrients for the growth of marine
mixotrophic planktonic protists and their role in the planktonic food web
1.1) The role of light, food concentration and nutrients for the growth of marine
mixotrophic planktonic protists
Mixotrophic protists with their own chloroplasts (=phytoflagellates)
Mixotrophic protists with chloroplasts of their own display a growth response towards
irradiance like an ordinary autotrophic alga. However their growth response to addition of
preys varies quite bit among species. In the case of the dinoflagellate Fragilidium
subglobosum, addition of prey will increase the maximum growth rate at all irradiances.
However, the difference in growth rates is by far largest at low irradiances and this species
can even grow quite fast in the dark if supplied with fresh food (Skovgaard 1996). Overall this
species grows equally well as a phototrophic and a heterotrophic. However, it grows fastest as
a mixotrophic. Some down regulation of the photosynthetic apparatus occurs when F.
subglobosum is well fed and the contribution of phagotrophy to the total carbon uptake is
more than 70%, even at high irradiances.
The prymnesiophytes Chrysochromulina spp. function completely different. In these
species the addition of even large amounts of prey will only affect the growth rate at low
Introduction
18
irradiances and thus food serves only as carbon supplement when light becomes limiting for
growth (Jones 1997, Hansen & Hjorth 2002). Feeding in these species does not seem to boost
the photosynthetic apparatus (Jones 1997). However, light is required for growth in
Chrysochromulina spp (Jones 1997). In other cases, like in the dinoflagellates Karlodinium
armiger and K. veneficum/micrum (Li et al. 1999, Berge et al. 2008a), the maximum growth
rate in a growth medium is quite low (between 0.01-0.25 d-1 depending upon species and the
use of soil extract) even at high irradiances. Addition of prey will lead to considerably higher
growth rates (0.7 – 0.94 d-1) at relative high irradiance. So in these species, feeding is very
much dependent upon light and they will not feed in the dark (Li et al. 1999). Feeding also
seems to boost the photosynthetic apparatus Karlodinium spp, as chloroplasts tend to increase
in size when the cells are fed (Berge et al. 2008a,b, Li et al. 1999).
The dinoflagellate Gymnodinim resplendens behaves very much like the Karlodinium
species. This species has chloroplasts of its own, but it can not grow in the light in an
inorganic nutrient medium, if it does not feed on another dinoflagellate, Prorocentrum
minimum. The ingested prey supplies the dinoflagellate with organic carbon as well as
essential growth factors for phototrophy growth. Like in Karlodinium, feeding seems to boost
the photosynthetic apparatus, as rates of photosynthesis go up when the cells are fed. In
contrast to Karlodinium spp, Gymnodinium resplendens can grow in the dark if fed, but with
much lower growth rates (Skovgaard 2000). An even more extreme example is represented by
mixotrophic dinoflagellates belonging to the genus Dinophysis. These species have
chloroplasts of their own (of either cryptophyte or haptophyte origin), but they cannot grow in
an inorganic medium without food (Riisgaard & Hansen 2009). All Dinophysis species in
culture so far have been grown with the ciliate Mesodinium rubrum as prey (Park et al. 2006,
Kim et al. 2008, Nishitani et al. 2008a, b, Cuetos-Gracia 2010). Food uptake may provide the
dinoflagellate with more than 70 % of its carbon needs (Riisgaard & Hansen 2009) However,
at natural prey concentrations food uptake will only make up for less than 10 % of its carbon
requirements (Riisgaard & Hansen 2009). Mixotrophic Dinophysis are dependent upon light
for growth (Kim et al. 2008, Riisgaard & Hansen 2009).
Some mixotrophic dinoflagellates only feed when they are nutrient limited and stop
feeding when nutrients become available e.g. Ceratium furca (Smalley et al. 2003). Likewise,
Heterocapsa triquetra and Procentrum minimum only ingest prey when inorganic nutrients
are limiting (Stoecker et al. 1997, Legrand et al. 1998) and ingestion of prey by the
Introduction
19
mixotrophic dinoflagellate Prorocentrum minimum is in fact inhibited by addition of nitrate
and phosphate (Stoecker et al. 1997).
Mixotrophic protist with symbionts and kleptochloroplasts
In the case that chloroplast do not originate from the mixotrophic itself. The photosynthetic
component in mixotrophic can be in different forms. “Symbiosis” and “kleptochloroplastidy”
have been found commonly among marine protists (e.g. Norris 1967, Taylor 1982, Laval-
Peuto & Rassoulzadegan 1988, reviewed by Stoecker 2009). “Symbiosis” refers to the
relationship between two or more organisms. Typically, a symbiont living inside (as
endosymbiont) or is attached to the outside/surface (as ectosymbiont) of another organism
(the host). Kleptochloroplastidy (or kleptoplastidy) is the process by which the chloroplasts of
a prey are sequestered by a predator and photosynthetically still active for a while in predator.
Thus, stolen chloroplasts are called kleptochloroplasts or just kleptoplastids (Schnepf 1993).
Mixotrophic protists with endosymbionts (hosts) tend to largely depend on the
photosynthetic activity of their endosymbiont. In many cases, however, the hosts need to
ingest prey to get essential substances for sustaining their symbionts. Therefore, when starved
under optimal light conditions, the photosynthetic activity supports and prolongs survival of
the hosts. An example of this type of symbiosis is found in the green Noctiluca scintillans,
which is a common bloom former in South East Asia. This species contains thousands of cells
of the pedinophyte Pedinomonas noctilucae. Some strains can grow photoautotrophically in
an inorganic medium with some vitamins added, while others strains of the green Noctiluca
scintillans are dependent upon ingestion of food (Hansen et al. 2004, Saito et al. 2006). The
phagotrophy can contribute as much as 30% to the growth rate of the green N. scintillans
under saturated prey concentrations and optimum light intensity. However, at natural prey
concentrations typically found where it blooms, the contribution of ingested prey is much
smaller (a few %; Hansen et al. 2004).
Another example of a marine protist with endosymbionts is the ciliate Mesodinium
rubrum. This ciliate contains a cryptophyte symbiont. However, it can only grow if supplied
with cryptophyte prey (Gustafson et al. 2000). It gets most of its carbon from photosynthesis,
very much like in the case of the green Noctiluca (Johnson & Stoecker 2005, Smith & Hansen
2007).
Introduction
20
Some dinoflagellates, within the order Dinophysiales (i.e. Ornithocercus, Histioneis),
bear cyanobacterial ectosymbionts (Norris 1967, Lucas 1991). However, the exact
relationship between ectosymbionts and their hosts is not known.
Some marine protists only sequester the chloroplasts from their prey. In all known
cases in marine waters, the marine kleptochloroplastidic protists depends on largely
phagotrophy for growth, while photosynthesis of the chloroplasts only seem to be enough to
cover for respiration and excretion. (Putt 1990a). The sequestered chloroplasts need to
replaced, because they only will be functional for a short time; from few to several days
depends on species (Stoecker & Silver 1990, Skovgaard 1998, Lewitus et al. 1999). Thus the
main role of kleptochloroplasts seems to be that they help the hosts to survive periods of low
prey abundances (Stoecker et al. 1988, Stoecker & Silver 1990, Skovgaard 1998,). An
example of such a protist is the ciliate Laboea strobila (Putt 1990a), but quite a few
“oligotrichs” have the same ability (e.g many Strombidium spp. ; Stoecker & Silver 1990,
Jonsson 1987). These ciliates are all able to grow in the dark, although their growth rates are
higher in the light. Among the dinoflagellates, kleptoplastidity has been reported for a number
dinoflagellates, i.e. Amphidinium poechilochroum (Larsen 1988; Gymnodinium graciletum
(Skovgaard 1998, Jakobsen et al. 2000), Cryptoperidiniopsis sp, and Pfiesteria piscicida (e.g.
Lewitus et al. 1999, Eriksen et al. 2002). Like for the ciliates, the growth rate of the
kleptoplastidic dinoflagellate Cryptoperidiniopsis sp. is strongly influenced by light intensity
when fed prey cells in excess (Storeatula major, cryptophyte).
1.2) Importance of marine mixotrophic protists in the planktonic food web
Scientists dealing the marine plankton food web often split protists into either
phototrophic protists (=phytoplankton) and heterotrophic protists (= protozoa) and relatively
few papers have incorporated mixotrophs into the foods web. One of the reasons for this is
that our knowledge on which species are mixotrophs is still somewhat limited. Another reason
is that it is difficult to “identify” mixotrophs in field samples. In many cases you will not be
able to apply a species name to fixed cells in water samples. In those cases, mixotrophy is
“confirmed” by addition of stained prey or plastic beads. In a few cases, where the grazing of
large protists has been considered, beads have been added to some smaller protists which then
again have been fed to the mixotrophs in question (Smalley & Coats 2002). Pitta &
Introduction
21
Giannakourou (2000) reviewed the obstacles in distinguishing mixotrophic from phototrophic
or heterotrophic protists with recently ingested algae. They also discussed the use of different
methodology in sampling and preserved which led either underestimated or overestimated
number of mixotrophic planktonic protists. Overall, however, mixotrophs are every where; it
is just a matter of identifying them and their impact on the food web.
Mixotrophic nanoflagellates can contribute up to 39 - 50 % of the phototrophic
nanoflagellates abundance in surface waters of Sargasso Sea (Arenovski et al. 1995, Sanders
et al. 2000). The prey of nanoflagellates is usually consisting of bacteria and in some cases
picoplanktonic algae. Zublov & Tarran (2008) revealed that the small mixotrophic flagellates
(<5μm) carried out 40-95% and 37-70% of the bacterivory in the euphotic layer of temperate
North Atlantic Ocean in summer and in the surface waters of the tropical North –East Atlantic
Ocean, respectively. Marine mixotrophic nanoplankton are also important grazers on
picoplankton (i.e. Synechococcus) or nanophytoplankton (Sanders et al. 2000, Tsai et al. 2007,
Chan et al. 2009). Safi & Hall (1999) revealed that the mixotrophic nanoflagellates
contributed 57 % of measured grazing impact on picophytoplankton-sized particles, 40% of
grazing on bacteria-sized particles and 55% of grazing on stained bacteria per day. Also, in
the northwestern Black Sea during the summer 1995, the mixotrophic planktonic protists
(nano- and microplankton) contributed to 14% and 24% of the ingestion of bacteria and
nanoplankton, respectively even though their biomass were significantly lower than
heterotrophic planktonic protists (Bouvier et al. 1998). Recently, Unrein et al (2010) estimated
that only one single mixotrophic species, Dinobyon faculiferum represent 4.5% of the total
bacterial grazing losses in coastal Mediterranean.
Very little is known about the role of mixotrophic ciliates and dinoflagellates as
grazers in natural communities. Smalley & Coats (2002) investigated the feeding rate of
dinoflagellate Ceratium furca in Chespeake Bay. They obtained feeding rates of 0-0.11 prey
C. furca-1h-1 and estimated that Ceratium furca alone removed on average 16% of
Choreotrichid (<20µm size) d-1and 67% of Strobilidium spp populations per day.
Li et al. (2000) revealed the mean number of ingested cryptophyte per Gyrodinium
galatheanum was high as 0.46 for G. galatheanum populations in the surface waters of the
mid- and upper of Chesapeake Bay. However, G. galatheanum has only minor grazing impact
on cryptophyte prey populations, removing about 0-4% of the cryptophyte standing stock on a
Introduction
22
daily basis. Mixotrophic oligotrich ciliates can form an important part of the photosynthetic
biomass. Estimates of their contribution to total chlorophyll range up to 24% for surface
samples from Nordic Seas (Putt 1990b). Mixotrophs can likewise make up for a substantial
biomass of grazers. Stoecker et al. (1996) estimated the biomass of mixotrophs (i.e. ciliates,
foraminifera, acantharia, polycystine radiolaria) in upper 90 m in Equatorial Pacific (at
1400W) made up for as much as 27%, 47% and 56% of the protozooplankton biomass in size
ranges 20-64 µm ,>64-200 µm and > 200µm, respectively and this does even not include the
mixotrophic dinoflagellates. Pitta & Giannakourou (2000) found that mixotrophic ciliates (i.e
mixotrophic oligotrichs) contributed 17-54% of ciliate abundance and 13-62% of ciliate
biomass in oligotrophic Eastern Mediterranean. Especially one mixotrophic ciliate tends to
dominate in many waters, Mesodinium rubrum. This species is primarily a phototrophic
mixotroph, but when it blooms it will have a huge impact on the occurrence of other protists
(Smith & Hansen 2007). Even in non bloom occurrences, Stoecker et al. (1991) reported
Mesodinium rubrum made up for from <1 to ≥ 70% of the community primary production in
the surface water.
One of the disadvantages of the methods used in the papers mentioned above is that
these techniques do not give the full picture of what the protists eat. Recently, a new
technique has been invented, which in principle will allow for the determination of more
realistic ingestion rates (i.e. a measure of the total amount of food ingested) (Rose et al 2004).
The principle of the method is that it uses a pH stain (a fluorescent acidotropic probe) which
will penetrate the predator cell and stain food vacuoles (with low pH) (Carvalho & Granéli
2006). Carvalho et al. (2008) combined this staining technique with flow cytometry to verify
Dinophysis norvegica feeding frequency (i.e. the percentage of cells containing food vacuoles
in a given population) in natural populations in the Baltic Sea. Using this technique a greater
number of D. norvegica cells can be examined in the short time and it allows the analysis of
live cells from cultures or natural populations in real time. Also, a much higher frequency of
food vacuoles were obtained with this technique than previously reported. The limitation of
the acidotropic probe technique is that it will stain other acidic vesicles (i.e. autophagic,
parasitophorus) and not only food vacuoles (Carvalho & Granéli 2006). Recently, Bowers et
al. (2010) combined flow cytometry and real-time PCR to demonstrate consumption of a
cryptophyte species (Rhodomonas sp.) by a toxic mixotrophic haptophyte (Prymnesium
parvum). Using flow cytometry, the feeding frequency of a population of P. parvum cells was
calculated using the phycoerythrin (PE) autofluorescence signal from Rhodomonas sp. and the
Introduction
23
fluorescence of an acidotropic probe labeling the food vacuoles. Thus, flow cytometry
allowed for a rapid enumeration of food vacuoles and enumeration of consumed prey cells
based on their pigments, while real-time PCR confirmed the identity of the species within the
food vacuoles. These recent advanced techniques (i.e. Carvalho et al. 2008, Bowers et al.
2010) now allow us to study the role of phagotrophy by mixotrophs in natural populations
much better.
Introduction
24
Part 2) Marine symbiont-bearing dinoflagellates
Part 2.1 Occurrence of symbionts in the order Dinophysiales
The dinoflagellate order Dinophysiales includes the families Amphisoleniaceae,
Dinophysiaceae, and Oxyphysaceae (Steidinger & Tangen 1997). So far, symbionts have only
been found associated with species within the families Amphisoleniaceae and Dinophysiaceae
(Table1). The family Amphisoleniaceae consists of the genera Amphisolenia and
Triposolenia, which both of which have species that contain endosymbionts of prokaryotic as
well as of eukaryotic origin (Kofoid & Skogsberg 1928, Lucas 1991). Recently, Daugbjerg et
al. (unpublished) revealed that symbionts of A. bidentata were in fact closely related to some
free-living pelagophytes.
Fig. 3 Some marine symbionts-bearing dinoflagellates in order Dinophysiales: a) Amphisolenia b) Triposolenia c) Citharistes d) Histioneis e) Parahistioneis f) Ornithocercus (Kofoid & Skogsberg 1928, Taylor 1976)
Introduction
25
In the family Dinophysiaceae, the genera Citharistes, Histioneis, Parahistioneis,
Ornithocercus , all bear ectosymbionts (Fig. 3). Originally, the ectosymbionts were named
“Phaeosomes” by Schütt (1895), who first gave this name to the brown coloured bodies he
discovered in the girdle list of these dinoflagellates. Norris (1967) was the first to identify that
these structures contained symbionts and after studying the living specimens, he finally
concluded that these symbionts were cyanobacteria. The species he found were named
Synechococcus carcerarius and Synechocystis consortia. Apart from these species he also
found some symbionts, which he thought might be eukaryotic algae. In 1991, Lucas identified
4 types of cyanobacterial symbionts by examination of the cytoplasmic content of cells (Lucas
1991). Foster et al. (2006b) later provided the first phylogenetic description of the diversity of
symbionts of dinophysoid genera (Amphisolenia, Citharistes, Histioneis, Ornithocercus).
They revealed that majority of the cyanobacterial symbionts sequences were most similar to
Synechococcus and Prochlorococcus, while some sequences had a low identity (<92%) to
eukaryotic chloroplasts. Some sequences were, however, more similar to a variety of
heterotrophic bacteria. In the same year, Foster et al (2006a) identified 8 different
morphotypes of cyanobacterial ectosymbionts and 2 bacteria morphotypes from
Ornithocercus spp. and Histioneis spp. based on TEM.
Other members of the family Dinophysiaceae include the genus Dinophysis, which
contains phototrophic species which have either cryptophyte (e.g. D. acuminta, D. acuta, D.
norvegica) or haptophyte (only D. mitra) chloroplasts (Table 1) . Molecular data have
suggested that Dinophysis spp. relies on sequestered chloroplasts from ingested prey
(kleptochloroplasts; e.g. Janson 2004, Minnhagen & Janson 2006, Nagai et al. 2008, Park et
al. 2008). Recently, Garcia-Cuetos et al. (2010), however, using a combination of molecular,
TEM and experimental techniques revealed that the chloroplasts of D. acuminata in fact are
chloroplasts, which are now fully incorporated in the dinoflagellate, and not
kleptochloroplasts, which the cells get from ingested prey. However, more research is needed
to clarify if this is true for all phototrophic Dinophysis spp. Nevertheless, in this thesis, the
Dinophysis spp. are not considered symbiont bearing.
Introduction
26
Table 1. Mixotrophy in marine Dinophysiales
?: Status not confirmed
Host species Status of symbiont/chloroplast
Symbionts species (or taxa)/chloroplast origin
Distribution References
Family Amphisoleniaceae Amphisolenia spp. endosymbiont Eukaryotic algae (chrysophyte? or
dinoflagellate?), cyanobacteria e.g. Synechococcus carcerarius, bacteria
Oceanic, sometimes associated with upwelling; cosmopolitan in warm temperate to tropical waters
Paper I & references therein
Pelagophyte species Daugbjerg et al. unpublished Triposolenia spp. endosymbiont? Small irregular, very pale yellowish-
green chromatophores Probably exclusive eupelagic; cosmopolitan in warm temperate to tropical waters
Paper I & references therein
Family Dinophysiaceae Citharistes spp. ectosymbiont Cyanobacteria Oceanic, tropical, subtropical and
warm temperate seas Paper I & references therein
Histioneis/ Parahistioneis spp.
ectosymbiont Eukaryotic algae, cyanobacteria e.g. Synechococcus carcerarius, Synechocystis consortia
Oceanic; cosmopolitan in warm temperate to tropical waters
Paper I & references therein
Ornithocercus spp. ectosymbiont Cyanobacteria e.g. Synechococcus carcerarius, bacteria
Neritic,Oceanic; cosmopolitan in warm temperate to tropical waters
Paper I & references therein
Dinophysis acuminata chloroplast yellow-orange autofluorescence, Neritic, typically cold and warm Lessard & Swift 1986, Cryptophyte origin temperate waters, worldwide Takishita et al. 2002, Garcia-Cuetos et al. 2010
Dinophysis caudata chloroplast Cryptophyte origin Neritic and estuarine in warm temperate to tropical waters, worldwide; rarely found in cold water, possibly an intruder in warm water masses
Park et al. 2008
Dinophysis fortii chloroplast Cryptophyte origin Oceanic and neritic; cold temperate to tropical waters, worldwide
Nagai et al. 2008, Takishita et al. 2002
Dinophysis infundibulus chloroplast Cryptophyte origin Temperate waters Nishitani et al. 2008b Dinophysis mitra chloroplast Haptophyte origin Temperate to tropical waters Koike et al. 2005
Dinophysis norvegica chloroplast yellow-orange autofluorescence, Neritic,Oceanic; temperate waters Lessard & Swift 1986 Cryptophyte origin Takishita et al. 2002
26
Introduction
27
Part 2.2 The spatial distribution of symbiont-bearing dinoflagellates in marine waters
The geographical distribution of symbiont-bearing dinoflagellates (i.e. Amphisolenia,
Citharistes Histioneis Ornithocercus, Parahistioneis, and Triposolenia) belonging to the order
Dinophysiales, is restricted to tropical, subtropical and warm-temperate seas from about 460N
to 400S (Table 6&Fig 10 in Paper I) and they are exclusively found in oligotrophic waters
(Gordon et al. 1994, Jyothibabu et al. 2006, Paper I). Very little is known about these
dinoflagellates as most of the papers published so far have been qualitative studies. The
reason for this is undoubtedly due to their scarce numbers even in their most favored offshore
environments (0.1-5 cells l-1) and to fact that none of them have been successfully established
in the laboratory cultures yet. Few researches have investigated the seasonal distribution of
symbiont-bearing dinoflagellates. Gordon et al. (1994) investigated the seasonal distribution
of ectosymbiont-bearing dinoflagellates (genera Ornithocercus, Hisitoneis/Parahistioneis and
Citharistes) during almost 5 years in the Gulf of Aqaba, Red Sea. They found that the
ectosymbiont-bearing dinoflagellates showed peak in numbers during periods characterized
by thermal stratification and nitrogen limitation. Similar results were obtained by Jyothibabu
et al. (2006) in the Bay of Bangal. Here the highest cell concentrations of ectosymbiont-
bearing dinoflagellates were found in the spring intermonsoon, which is characterized by
strong thermal stratification and nitrogen depletion in the upper water layers. To the best of
my knowledge, there are no reported on the temporal distribution of endosymbiont-bearing
dinoflagellates.
In Paper I, I investigated the horizontal and vertical distribution of symbiont-bearing
dinoflagellates along the Indian Ocean and in Broome Sea, Australia. My study revealed that
the highest cell concentrations and the highest species diversity of symbiont-bearing
dinoflagellates were found at surface temperature between 20-300C and associated with low
nitrogen concentrations (N-limitation). However, the symbiont dinoflagellates were not
common in coastal waters, with high turnover rates of primary production, even though
nitrogen concentrations were low. This indicates that turnover rates of nutrients also play a
role. Symbiont-bearing dinoflagellates have typically been found in surface waters down to
100-400 m’s depth (Kofoid & Skogsberg 1928, Gómez 2005, Paper I). Paper I suggested
that their vertical distributions were related to the depth of optimal irradiance for the growth
of the symbionts (in the upper 100 m). However, other factors (i.e prey cells) may play a role
to in constraining the vertical distribution of the symbiont-bearing dinoflagellates.
Introduction
28
Part 2.3. The role of symbionts and phagotrophy in dinoflagellates with symbionts
What do the otherwise heterotrophic dinoflagellates get out of having ectosymbionts? Light
microscopical photographs (Fig 7 in Paper I) showed food vacuoles inside the
dinoflagellates, which had resemblance in size and color to their symbionts. TEM pictures
confirmed food vacuoles inside the dinoflagellates, but the remains could not in most cases be
identified. However, one food vacuole revealed remnants of a chloroplast close to trichocysts.
The trichocysts seemed to come from a ciliate and thus it was speculated that this was remains
from a ciliate, which had ingested a eukaryotic prey (Fig 9 in Paper I). Likewise, Lucas
(1991) found that some cells of Ornithocercus magnificus, Histioneis dolon and Parahistineis
para contained food vacuoles with unidentified prey inside. Unfortunately, no direct
observations of the ingestion process in symbiont-bearing dinoflagellates have been observed
so far.
The ectosymbionts in Dinophysiales are cyanobacteria and nitrogen fixation has been
shown among marine strains of small unicellelular cyanobacteria: Synechococcus,
Synechocystis, Cyanothece and Gleocapsa sp. (Spiller & Shanmugam 1987, Reddy et al
1993). Since the cyanobionts of symbiont-bearing dinoflagellates have been shown to be
closely related to the N2 fixing genera Synechococcus and Cyanotheca, it seems likely that
they indeed fix nitrogen (Foster et al 2006b). Also, it is well known that high temperatures
and N-limitation seem to promote nitrogen fixation in non heterocyst diazotrophic
cyanobacteria (Gordon et al.1994, Capone 2000, Karl et al. 2002, Staal et al. 2003, Breitbarth
et al. 2006). It may in fact be one of reasons why the cyanobiont-bearing dinoflagellates are
restricted to warm nutrient depleted waters.
So far, cyanobacteria symbionts have been found as ectosymbionts in the genera
Ornithocercus spp., Histioneis spp., Parahistioneis, Citharistes spp. and confirmed as
endosymbionts in Amphisolenia spp. (Norris 1967, Lucas 1991, Janson et al. 1995, Foster et
al. 2006a, b, Paper I, Appendix-Paper IV). However, Janson et al. (1995) did not detect the
nitrogenase (nitrogen fixing enzyme) from cyanobionts of Ornithocercus spp. by labeling
antisera against the enzyme nitrogenase, which is involved in nitrogen fixation. Interestingly,
the cyanobiont type 4 of Histioneis depressa is so far the only one, which has been shown to
be capable of N2 fixation (Foster et al 2006a). Recently, Farnelid et al. (Appendix-Paper IV)
Introduction
29
revealed that some heterotrophic bacteria, which were found in association with the symbiont-
bearing dinoflagellates, were the ones containing the nifH genes. Much more research is
however required on this topic
Some Amphisolenia species (i.e A. bidentata, A. thrinax), possess eukaryotic
endosymbionts (Lucas 1991, Appendix-Paper IV, Daugberg et al. umpublished). The
symbionts appear to be completely intact eukaryotic cells (pelagophytes). No food vacuoles
have been found in Amphisolenia species, indicating the species do not digest their symbionts
and thus rely on photosynthetate leaking from the symbionts (Lucas 1991).
Introduction
30
Part 3) Symbiosis and mixotrophy in the marine ciliate genus Mesodinium
Part 3.1. Occurrence of symbiosis in Mesodinium spp.
The ciliate genus Mesodinium (Stein 1863) is a small genus belonging to the order
Cyclotrichiida (Class Litostomatea; Lynn 2008). This genus is globally distribution in aquatic
habitats and includes species which are mixotrophic as well as completely heterotrophic. The
mixotrophic species (the red M. rubrum (Lohmann 1908) Hamburger & Buddenbrock 1911
(=Myrionecta rubra), possesses a symbiont which originates from the Teleaulax /Geminigera
clade (Hansen & Fenchel 2006, Garcia-Cuetos et al. 2010). This species is found in saline
lakes, brackish and marine waters (Lindholm 1985, Perriss et al. 1995). Isolates of
Mesodinium rubrum are now in culture from Antarctic waters (Gustafson et al 2000) and
temperate waters (Korea: Yih et al. 2004, Denmark: Hansen & Fenchel 2006). To what extent
all these isolates are the same species is unknown at present.
Heterotrophic non-symbiont containing species i.e. M. pulex Claparède & Lachmann
1858,1859, M. acarus Stein 1867, M. fimbriatum Stokes 1887, M. cinctum Calkins 1902, M.
pupula Kahl 1933, and M. velox Tamar 1986 have been found in both freshwater and marine
waters, except for M. velox, which has only been reported from marine habitats (Tamar 1986,
Foissner et al. 1999). Interestingly, some species (i.e. M. pulex, M. acarus) have been found in
both planktonic and benthic habitats. However, M. pulex can easily be confused with M.
acarus and M. fimbriatum (Noland 1937, Foissner et al. 1999). Recently, Bass et al. (2009)
discovered that clones of so-called M. pulex were located in two different phylogenetic clades,
indicating the existence of an additional undescribed species. Clearly, more research on the
species diversity in this genus is needed.
Part 3.2. The distribution of marine Mesodinium spp.
Marine Mesodinium spp. includes species without symbionts i.e. M. pulex, M. acarus ,
M. pupula and M. velox and the symbiont containing M. rubrum (Lindholm 1985, Tamar
1986, Tamar 1992, Madoni 2006). Best known among the species without symbionts is M.
pulex, which is common in brackish and marine waters and the only one of this type which is
established successfully in laboratory cultures (Dolan &Coast 1991, Jakobsen et al. 2006,
Table 2).
Introduction
31
Table 2 Occurrence of Marine Mesodinium spp.
Species Length
(μm) Width (μm) (x103μm3)
Abundance (cells l-1) (mean) Living form Location references
Heterotrophic species M. .acarus - - - - benthic Lesina lagoon, Adriatic Sea Madoni 2006
- - - - benthic Chernaya River estuary,Kandalaksha Bay,
White Sea Mazei & Burkovsky 2005
M. pulex 18 - 1.3 - planktonic pond in L'Houmeau, near La Rochelle, French Atlantic coast
Dupuy et al.2007
- - - 15x103-≥ 3.00x105 benthic Molenplaat, Schelde estuary, Netherlands Hamels et al. 2005 20 15 345-6250 planktonic solar saltern of the Yellow Sea, China Lei et al. 2009
- - - - benthic Chernaya River estuary,Kandalaksha Bay,
White Sea Mazei & Burkovsky 2005
- - - - planktonic Baltic Sea Mironova et al. 2009 18-25 15-18 - - planktonic Yellow Sea, China Zhang et al. 2002 - - - - planktonic Nervión River estuary, Bay of bisca, Spain Urrutxurtu et al. 2003 12 - - - planktonic Oyster farming area, Thau Lagoon, France Dupuy et al. 2000 M. pulex var pupula Kahl 1933
- - - - benthic Chernaya River estuary,Kandalaksha Bay, White Sea
Mazei & Burkovsky 2005
M. pupula 25-30 18-20 planktonic Yellow Sea, China Zhang et al. 2002 - - - - planktonic Baltic Sea Mironova et al. 2009 M.velox 40 18 - 12 planktonic solar saltern of the Yellow Sea, China Lei et al. 2009 Phototrophic species M. rubrum 20 - - 1134 planktonic solar saltern of the Yellow Sea, China Lei et al. 2009 25-30 20-25 - - planktonic Yellow Sea, China Zhang et al. 2002
38 - 9.3 - planktonic pond in L'Houmeau, near La Rochelle,
French Atlantic coast Dupuy et al.2007
30 - - 180 (a) planktonic Wester basin of the Mediterranean Sea Dolan & Marrasé 1995
- - - 1.0x106 (a) planktonic Southampton Water and Test and Itchen
estuaries, England Crawford et al. 1997
M. rubrum large 33 28 - 402-8794 planktonic open northen Baltic Sea proper Johansson et al. 2004 M. .rubrum small 17 14 - 1257-9487 planktonic open northen Baltic Sea proper Johansson et al. 2004 M. rubrum - - - 50-3.7x104 planktonic estuary of the Gulf of Main, USA Sanders 1995 - - - - planktonic Nervión River estuary, Bay of bisca, Spain Urrutxurtu et al. 2003
(a) maximum concentration
31
Introduction
32
The symbiont containing M. rubrum forms regularly blooms in many areas i.e.
coastal, upwelling zones around the world (e.g. Stoecker et al.1989, Lindholm 1985,
Wilkerson & Grunseich 1990, Williams 1996, Table 2), and thus has received much more
attention than other marine Mesodinium spp (Crawford et al. 1997).
Mesodinium rubrum is generally present throughout the water column down to 200 m
(i.e. Lindholm 1985, Dolan & Marrasé 1995), however it can form dense patches in certain
parts of the water column, which is probably due to that fact that M. rubrum exhibits a strong
phototactic response (e.g. Lindholm 1985, Fenchel & Hansen 2006). The swimming
behaviour of M. rubrum is quite different from most other ciliates. Quick jumps are often
succeeding periods where the ciliate does not move. During jumps M. rubrum can travel at a
speed exceeding 5 mm s-1 (Lindholm 1985) and during jumps M. rubrum can reach a speed
up to 1.2 cm s-1(Fenchel & Hansen 2006). Mesodinium rubrum usually blooms in surface
waters, however blooms have been found subsurface (e.g. Lindholm 1985, Owen et al. 1992).
The factors related to subsurface bloom formation may be availability of nutrients or a
negative phototaxic response of M. rubrum to very high light intensities which can be found
in surface waters (Fenchel & Hansen 2006, Stoecker et al. 2009). Surprisingly, Montagnes et
al. (2008) found no relationship between M. rubrum abundance and suspected prey
(cryptophyte) in the open waters of the North Atlantic, but instead found a correlation
between abundance and temperature in the spring.
Most quantitative data on M. pulex come from freshwaters, i.e. lakes, reservoirs,
where it can be found in sediments and in ground waters as well (Barbieri&Godinho-Orlandi
1989, Wiackowski et al. 2001, Zingel et al. 2007,Andrushchyshyn et al. 2007). Very few
investigations have related M. pulex distributions to biotic or abiotic factors, but for example,
Gomes & Godinho (2003) found high abundances of M. pulex in a eutrophic lake near the
sediment, characterized by low dissolved oxygen levels. Also, it was found that M. pulex can
be positively correlated to chlorophyll a concentrations in highly eutrophic shallow lakes, like
in the Lake Köyliönjärvi (SW Finland) (Wiackowski et al. 2001). Although Mesodinium
pulex is a common ciliate in marine waters, quantitative data are rare and mostly derive from
investigation of ciliate fauna associated with the sediment. Reported cell densities range from
345-6250 cells l-1 in the solar saltern (10-30 cm deep) of the Yellow Sea (Lei et al. 2009) to I
5x103 to ≥3.00x105 cells l-1 in sandy sediments of an estuarine intertidal flat (Table 2, Hamels
et al. 2005).
Introduction
33
Part 3.3. The role of symbionts and phagotrophy in marine Mesodinium rubrum and
Mesodinium pulex
During the 1970’s it was shown that Mesodinium rubrum contains cryptophyte chloroplasts,
nucleomorphs, cryptophyte mitochondria, cryptophyte cytoplasma and a so-called symbiont
nucleus (Taylor et al. 1971, Hibberd 1977). Later, molecular data revealed that this symbiont
is very similar to (or identical) free-living cryptophytes belonging to the
Teleaulax/Geminigera clade and it was suggested that the ciliate got its chloroplasts and
symbiont nuclei from ingested prey (e.g. Johnson & Stoecker 2005, Johnson et al. 2006).
However, about the same time it was shown that M. rubrum can divide its chloroplasts and at
least in some isolates (Danish) also its symbiont nucleus (Hansen & Fenchel 2006, Garcia-
Cuetos et al. 2010). At present we do not know if the different isolates of M. rubrum in fact
are the same species, but the different results obtained with the Danish and Antarctic strains
may suggest that they are not the same species. Nevertheless, there is an ongoing debate
whether or not the symbionts in M. rubrum are permanent or indeed replaceable (i.e.
Gustafson et al. 2000, Hansen & Fenchel 2006, Johnson et al. 2007, paper III).
Photosynthesis is the main source of the nutrition of all studied M. rubrum strains (e.g.
Stoecker et al. 1991, Smith & Hansen 2007) and the ingestion of just 1 cryptophyte cell per
day is enough for M. rubrum in order to maintain its maximum growth rate. Besides this, the
symbiont allows M. rubrum to survive for extended periods (months) of time in the light
when subjected to sudden starvation (Johnson & Stoecker 2005, Smith & Hansen 2007). The
role of light for the non-symbiont containing Mesodinium species has never been investigated.
It is possible that the often so-called heterotrophic Mesodinium species in fact do carry out
some photosynthesis, which might increase their growth rate in the light or allow them to
survive longer in the light when starved. In Paper II, this was investigated for the first time
using M. pulex. It turned out that photosynthetic rates of M. pulex were quite low and never
exceeded 4% of the total carbon requirements of M. pulex.
Phagotrophy is important for both M. rubrum and M. pulex although it plays quite
different roles in the two species (i.e. Johnson & Stoecker 2005, Smith & Hansen 2007,
Paper II, Paper III). Mesodinium rubrum is able to feed on a variety of prey items, i.e.
cryptophytes, dinoflagellates and bacteria (Gustafson et al. 2000, Yih et al. 2004, Myung et al.
2006, Smith & Hansen 2007, Park et al. 2007, Paper III). However so far, M. rubrum has
Introduction
34
only been maintained successfully when fed cryptophytes, belonging to the
Teleaulax/Geminigera clade (i.e. Danish isolate: Teleaulax amphioxeia, Teleaulax sp., Korean
isolate:Teleaulax spp. and Antarctic isolate: Geminigera cryophila, Table 4 in Paper III).
Maximum growth rates of M. rubrum are obtained at very low prey concentrations (1000 prey
cells ml-1) and at very low food uptake rates (1-2% of total carbon uptake; Smith & Hansen
2007). Why M rubrum has to feed it still not fully resolved. Either, Mesodinium rubrum needs
to ingest a “growth factor”, which it only can get from the ingestion of specific species of
cryptophytes, or it needs to replace its symbionts by eating specific cryptophyte species from
time to time (Paper III).
Marine Mesodinium pulex gets most of its nutrition from phagotrophy (Paper II), and
thus maximum ingestion rates of M. pulex are far higher than that of M. rubrum (10-20 times;
Table 3, Smith & Hansen 2007, Paper II, Paper III). Like in the case of M. rubrum, M.
pulex ingests many different types of preys, including cryptophytes, dinoflagellates and
ciliates (Dolan &Coats 1991, Jakobsen et al. 2006, Paper II). Maximum growth rates of
Mesodinium pulex are higher than those of the symbiont containing M. rubrum, when
incubated in the sufficient light and prey concentrations (Smith & Hansen 2007, Paper II).
However, M. rubrum survives much better periods of starvation than M. pulex does. All
Mesodininum species catch their prey by the use of tentacles which are equipped with
mucocysts. Despite this, M.rubrum and M.pulex displayed quite different preferences for prey
species (Paper II, Paper III).
Table 3 Comparison of ingestion rates and growth rates between M.rubrum and M.pulex when fed on different prey M.rubrum M.pulex
Prey species Ingestion rate ± SE Growth rate ± SE Ingestion rate ± SE Growth rate ± SE (cells predator-1 d-1) (d-1) (cells predator-1 d-1) (d-1) Heterocapsa rotundata 0.84±0.11 0.08±0.04a 14.93±0.85 1.13±0.03 Guillardia theta 0.43±0.01 0.37±0.01 6.51±2.80 -0.10±0.29 Hemiselmis tepida 0.47±0.02 0.32±0.01 4.21±2.63 -0.37±0.03 Monoculture of Predator - 0.11±0.04a - -0.65±0.09 - 0.31±0.02 - - a : the same experiment
Introduction
35
Interestingly, light seems to affect ingestion and growth rates of the marine non-
symbiont containing M. pulex, which is unexpected for so-called heterotrophic protists (Paper
II). Most studies of functional and numerical responses of heterotrophic protists have been
carried out in dim light or in the dark (e.g. Verity 1991, Montagnes et al. 1996, Strom &
Morello 1998, Jeong et al.1999, Gismervik 2005). When supplied with sufficient prey, the
maximum ingestion rate of M. pulex was 49 prey cells predator-1 d-1 in the light, while it in the
dark the maximum ingestion rate was considerably lower (27 prey cells predator-1 d-1). As a
consequence the maximum growth rate of M. pulex in the light was 1.41±0.03 d-1, as
compared to 1.19±0.07 d-1 in the dark (Paper II). Effects of light on ingestion and growth
may be due to both direct effects of light on digestion rates and to indirect effects due to light
effects on food quality (Skovgaard 1998, Strom 2001). The experiments carried out on M.
pulex (Paper II) were not designed to differentiate between direct and indirect effects of light.
However, the starvation experiments gave some indication that it may well be direct light
effects we observed. To our surprise we found higher mortality rates of M. pulex in the light
than in the dark (Paper II). This is in contrast to previous studies on heterotrophic protists
which showed no effect of light on survival rates when they were starved (Skovgaard 1998,
Strom 2001). It is well known that M. pulex can ingest prey of its own size (Dolan & Coats
1991) and recently, Moestrup et al. (in prep) found cannibalism in marine M. pulex. Therefore
if M. pulex has higher ingestion rates in the light than in the dark, this may explain the higher
mortality rates in the light compared to in the dark.
Introduction
36
Conclusion and future perspectives
Protists which mix phototrophy and phagotrophy (mixotrophs) are common and important
members of the marine plankton around the world. They occur in small numbers in
oligotrophic tropical offshore waters as well as in coastal eutrophic waters where they
sometimes form blooms. They potentially play great role both as primary producers and as
grazers on bacteria and other protists. This thesis focuses on distribution and biology of
protists which form symbiosis with phototrophic protists and bacteria. I have mainly worked
with two groups little studied groups: 1) Dinoflagellates which bear ecto and endo-symbionts
and 2) Ciliates with cryptophyte endosymbionts
Quite a few genera belonging to the class Dinophysiales contain species which have
either ectosymbionts (cyanobacterial) or intact endosymbionts (cyanobacterial or eukaryotes).
This study revealed that the species diversity and cell concentrations were highest in the
photic zone in offshore warm waters characterized by low nitrogen concentrations and low
turnover rates. They seem to live where most algae cannot make a living. We found evidence
for that the dinoflagellates bearing ectosymbionts ate their symbionts (ectosymbionts) as well
as other prey (ciliates). Besides this we found nifH genes associated N2 fixation in the
consortia. The big surprise was that these genes were not associated with the cyanobacteria,
but rather with heterotrophic bacteria, which were attached to the dinoflagellates. This group
of dinoflagellates has still not been cultured and we do not know much their biology.
Questions like: 1) how fast do these consortia grow, 2) how much of the carbon need of the
dinoflagellate come from the ectosymbionts, 3) what are the rates N-fixation and how
important is this for the success of the consortia, remains to be answered. We also found some
dinoflagellates (Amphisolenia spp) which had endosymbionts. The functional importance of
these endosymbionts to the dinoflagellates is completely unknown.
The ciliate genus Mesodinium contains species with cryptophyte endosymbionts as
well as species without symbionts. The study on the symbiont containing species Mesodinium
rubrum dealt with to what extent it can replace its symbionts or not. Despite feeding it with
different cryptophytes belonging to all major marine cryptophyte clades, we found no proof of
the ability of this ciliate to be able to exchange its symbionts. It would feed and digest all the
offered preys, but it could not sustain growth of any of them, except for when fed a Telaulax
Introduction
37
clade species. So either it can only exchange symbionts within this clade or it needs a growth
factor which it can only get by preying on Teleaulax clade species. Future studies should test
if it can exchange symbionts via ingestion of prey cells by offering it different species (which
can be separated genetically) within the Teleaulax clade.
I also studied the non symbiont containing Mesodinium species, M. pulex, in order to
test if photosynthesis is of any importance to such species. It turned out the photosynthesis
plays a little role for its carbon metabolism. Instead, to my surprise I found that ingestion rates
and growth rates were indeed increased in the light as compared to in the dark. This finding
has potential huge implications for our understanding on how light influences digestion rates
in large heterotrophic protists. If this is a general phenomenon, most published rates of
heterotrophic protists may have been underestimated a great deal.
Over the past decades, the studies on the functional biology of mixotophic protists
have revealed that mixotrophy is common among marine protists. However, due to
methodological problems in quantifying food uptake in natural communities, the role of
mixotrophs in marine food webs is not well understood. New promising tools like the
acidotropic stains, which in principle allow for detection of food vacuoles in protists in
general, have recently been developed. Combined with modern flow cytometry and molecular
tools, it may for the first time allow us to proper quantify food uptake in mixotrophs in natural
communities.
Introduction
38
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© Reprinted with kind permission from Inter-Research
PAPER I
PAPER II
Prey selection, ingestion and growth responses of the common
marine ciliate Mesodinium pulex in the light and in the dark
Woraporn Tarangkoon1,2, Per Juel Hansen1*
1Marine Biological Laboratory, Strandpromenaden 5, DK-3000 Helsingør, Denmark 2Faculty of Science and Fisheries Technology, Rajamangala University of Technology Srivijaya, 92150 Trang, Thailand *Corresponding author. E-mail [email protected]
ABSTRACT
The ciliate Mesodinium pulex (Class Litostomatea) ingested all 5 species of cryptophytes and the
autotrophic dinoflagellate Heterocapsa rotundata offered as prey. Despite this, it could only
grow on the cryptophytes Teleaulax sp. and Guinardia theta and the dinoflagellate H. rotundata,
because ingestion rates of the other prey cells, even at very high prey concentrations, were too
low to support growth. The numerical and functional responses of Mesodinium pulex fed
Heterocapsa rotundata were investigated in the laboratory in the light (100 μmol photons m-2 s-1)
and in the dark. In the light the growth rate was significantly higher than in the dark at all prey
concentrations. Active photosynthesis was measured in M. pulex and the measured rates could
not explain the increased growth rates in the light. Instead, the increased growth rates could
mainly be explained by elevated ingestion rates in the light. We also studied the starvation
response at different irradiances and in the dark (100, 50 μmol photons m-2 s-1 and dark). M.
pulex survived for up to 2 weeks without food, but mortality rates of in the light were larger than
in the dark.
Key words: Mesodinium pulex, growth, ingestion, ciliate
Tarangkoon & Hansen
1
INTRODUCTION
The ciliate genus Mesodinium (Stein 1863) is a small genus belonging to the Order Cyclotrichiida
(Class Litostomatea; Lynn 2008). Presently, the genus includes the red M. rubrum (Lohmann
1908) Hamburger & Buddenbrock 1911 (=Myrionecta rubra), which forms symbiosis with
cryptophytes belonging to the Teleaulax /Geminigera clade (i.e. Garcia-Cuetos et al. 2010) and 6
species without symbionts: M. pulex Claparède & Lachmann 1858,1859, M. acarus Stein 1867,
M. fimbriatum Stokes 1887, M. cinctum Calkins 1902, M. pupula Kahl 1933, and M. velox Tamar
1986.
Mesodinium acarus, M. fimbriatum and M. pulex have all been reported from freshwater,
but at least M. acarus and M. pulex also occurs in brackish and marine waters (i.e. Foissner et al
1999). The remaining species are commonly found in brackish and marine waters (Lindholm
1985, Foissner et al. 1999). Recently, Bass et al. (2009) reported that clones identified as M.
pulex fall into two different phylogenetic clades, indicating the existence of additional
undescribed species.
Our knowledge of which diet supports the growth of Mesodinium spp derives mainly
from studies of M. rubrum and M. pulex. Mesodinium rubrum has been shown to ingest many
different species of cryptophytes (Gustafson et al. 2000, Yih et al. 2004, Park et al. 2007, Hansen
et al. in prep). However, it can also feed on other types of prey (i.e a dinoflagellate; Hansen et al.
in prep). Despite this, successful cultures of Mesodinium rubrum have so far only been
established on cryptophytes within the Teleaulax clade as prey (i.e Teleaulax amphioxeia, T.
acuta and Geminigera cryophila; Gustafson et al. 2000, Yih et al. 2004, Hansen & Fenchel 2006,
Park et al. 2007, Hansen et al in prep). Mesodinium pulex has so far been reported to feed on the
dinoflagellate Heterocapsa rotundata and the ciliate Metanophrys sp and successful cultures
have been established on both types of prey (Dolan & Coats 1991, Jakobsen & Strom 2004,
Jakobsen et al. 2006). It has been shown to be a very inefficient grazer on the cryptophyte
Rhodomonas salina or the dinoflagellate Gymnodinium simplex, mainly due a very low
successful capture rate. Also it will not ingest artificial beads (Dolan & Coats 1991, Carrias et al.
1996). A recent study has suggested that M. rubrum also ingests bacteria (Myung et al. 2006);
Tarangkoon & Hansen
2
however the capture mechanism of the small sized bacteria is unknown at this stage and it is
currently unknown if M. rubrum can sustain growth on bacteria.
Detailed physiological studies have only been carried out on temperate strains and an
antarctic strain of Mesodinium rubrum (Yih et al. 2004, Johnson & Stoecker 2005, Hansen &
Fenchel 2006, Park et al. 2007, Smith & Hansen 2007). This species, which forms symbiosis with
cryptophytes, is predominantly phototrophic and can only grow in the light. Maximum growth
rates are obtained at very low prey concentrations (1000 prey cells ml-1) and at very low food
uptake rates (1-2% of total carbon uptake; Smith & Hansen 2007). When subjected to sudden
starvation, M. rubrum will perform 3-4 cell divisions and to a large extent replicate its
cryptophyte chloroplasts and at least in some isolates also its symbiont nucleus (Hansen &
Fenchel 2006, Johnson et al. 2007, Smith & Hansen 2007). Thereafter it is able to starve for up to
a month depending on the incubation temperature. Very little is known about the physiology of
the non-symbiont containing species, and their photosynthetic potential has never been studied
(Jakobsen 2006). However, prior to this study we observed that M. pulex cells often are filled
with autotrophic food even after the prey has been depleted, indicating possible active
photosynthesis.
The aim of the present work was to study the prey selection and feeding physiology of a
non-symbiont containing Mesodinium species. We chose M. pulex because it is a very common
species around the world and because it is the only species which at the moment can be held in
laboratory culture. We hypothesized that: 1) Mesodinium pulex is an omnivorous feeder that
relies on its ability to sense and successfully catch prey cells. 2) Mesodinium pulex can carry out
photosynthesis in the light and that this carbon uptake in light will increase its growth rate or
make it survive better when suddenly starved.
MATERIALS AND METHODS
Culture of organisms. Mesodinium pulex was provided by the culture collection of the Marine
Biological Laboratory (Helsingør, Denmark). It had originally been isolated from water samples
collected from a boat launch near Shannon Point Marine Center, Washington, USA by H.H.
Tarangkoon & Hansen
3
Jakobsen (Jakobsen et al. 2006). Cultures of M. pulex were maintained on the dinoflagellate
Heterocapsa rotundata unless otherwise stated. The organisms were grown at a temperature of
20+1 0C in enriched filtered (0.22µm) f/20 seawater medium (Guillard 1983) at salinity 30+1 psu
without silicate. Light was provided by cool-white fluorescent light at a light:dark cycle of
14:10h with an illumination of ca. 100 µmol photons m-2 s-1. Other prey items (cryptophytes,
Table1) used in some experiments were grown in enriched filtered h/20 seawater media
(modified from medium ‘h/2’ Guillard 1975), otherwise the growth conditions were as stated
above. All cultures were non-axenic.
Table 1. Algae used in the experiments, their cell volumes, estimated spherical diameters (ESD; n=20),
and culture identification.
Cell volume. Cell length and width of preserved organisms were measured in a Sedgewick-
Rafter chamber under an inverted microscope at 400x magnification. Suitable geometrical forms
of organisms were used and the cell volumes were calculated according to Hillebrand et al.
(1999). The following geometrical forms were used: A cone with hemisphere was used for M.
Species (taxa) Cell volume±SE
(µm3)
ESD
(µm) Culture collection –ID number
Heterocapsa rotundata
(Dinophyceae) 142.2±12.8 6.3 SCCAP-K0441
Teleaulax amphioxeia
(Cryptophyceae) 127.4±9.5 6.1 SCCAP
Teleaulax sp.
(Cryptophyceae) 105.3±2.9 5.8 MBL - 1
Guillardia theta
(Cryptophyceae) 100.7±4.5 5.6 CCMP-2712
Hemiselmis rufescens
(Cryptophyceae) 72.4±4.3 5.0 CCMP-440
Hemiselmis tepida
(Cryptophyceae) 58.2±4.8 4.7 CCMP-442
Tarangkoon & Hansen
4
pulex, H. rotundata, Teleaulax sp. and T. amphioxeia, while the shape of polate spheroid was
used for G. theta, H. rufescens, H. tepida. The ESD (Equivalent Spherical Diameter) was
estimated by the equation: ESD = (biovolume/0.523)0.33. Carbon contents of M. pulex and the
prey organisms were estimated with the carbon conversion factor: pg C cell-1 = 0.216 x
volume0.939 and pg C cell-1 = 0.760 x volume0.819, respectively (Menden-Deuer&Lessard 2000).
Feeding and growth of M. pulex fed different prey items. This experiment was designed to test
whether M. pulex was able to feed and grow on a variety of cryptophytes and a dinoflagellate
(Table 1). For comparison we ran control experiments with M. pulex in monocultures (thus
exposed to starvation). Preliminary experiments had shown that some of the cryptophytes cannot
grow on the f/20 medium, because they cannot use nitrate as an inorganic N-source. We therefore
switched to the medium “h/20”, which is basically the same as f/20, except that it also includes
ammonium (Guillard 1975). Prior to the initiation of the experiments, M. pulex had been grown
on H. rotundata and allowed to deplete H. rotundata as prey (= residual prey concentrations less
than ca < 5 cells ml-1). This experiment was carried out at an irradiance of 100 µmol photons m-2
s-1.
Preliminarily, the initial predator-prey concentration ratio was set at ca. 25:1250 cells ml-
1, to ensure sufficient prey in mixed culture during the experiment period (4 days). However, it
turned out that M. pulex could not control the cryptophyte prey populations. We therefore used an
initial predator-prey concentration ratio of ca. 200:1000 cells ml-1 (1:5 ratio) on Day 0.
Monocultures of cryptophytes were initiated at a concentration of ca. 1000 cells ml-1. Subsamples
(5-10ml) were withdrawn at Day 0, 2 and 4 from triplicates of 65-ml tissue culture bottles and
cells counted. After subsampling on Day 2, all experimental bottles were refilled to capacity with
fresh filtered h/20 medium. In cases where prey populations had been almost depleted during the
first 2 days of the experiment, additional prey was added to the experimental bottles. Only the
experiments in which M. pulex displayed growth between Day 2 and 4 were allowed to continue
to Day 6. pH was measured in all experimental bottles during experimental period with a
Sentron® pH meter (model 2001) equipped with Red line probe, with a detection limit of 0.01
pH units. The pH meter probe was calibrated with Sentron buffers of 7 and 10.
Tarangkoon & Hansen
5
The growth/mortality rate of M. pulex ( yμ ) and prey cells ( xμ ) was calculated assuming
exponential growth/mortality:
tNN
dxy)ln(ln)( 011
,−
=−μ
where N0 and N1 are cell number at start (t0) and at the end (t1) of each incubation experiment,
respectively, and t is the interval time experiment (d).
The ingestion rate U is the per capita ingestion rate, which is dependent on prey (x). This
was estimated using the following 2 equations.
Uydtdx
x −= μ
ydtdy
yμ=
Ingestion rate of M. pulex was calculated assuming that predator (y) and prey (x) grow
exponentially with rate constant of yμ and xμ , respectively. The decrease in prey due to grazing
isUy . This ingestion rate was calculated by using software as described in Jakobsen & Hansen
(1997).
Rates of growth and ingestion of M. pulex were fitted to the Michelis-Menten equation,
respectively, using the software SigmaPlot 10 (Systat Software, Inc) :
)x(xK)x(xμ
dm 0
0max1)(−+−
=−μ
Where maxμ is the maximal growth rate of M. pulex, x is the actual prey concentration, 0x is the
threshold prey concentration for growth (where yμ = 0) and mK is the prey concentration
sustaining ½ maxμ ,and:
(x)K(x)U
dUm +
=− max1)(
Tarangkoon & Hansen
6
Where maxU ís the maximal ingestion rate per M. pulex (prey cells predator-1 d-1), x is the prey
concentration (cells ml-1) and mK is the prey concentration sustaining ½ maxU
For statistic analysis, t-test analyses were used to test for differences found in rates between dark
and light treatments. T-test and ANOVA were used to compare means (ingestion rate and growth
rate) between each prey to zero value and between each other, respectively.
Photosynthetic performance of Mesodinium pulex and the prey Heterocapsa rotundata. The
photosynthetic performance of Mesodinium pulex cultures when fed the dinoflagellate
Heterocapsa rotundata was measured at an irradiance of 100 µmol photons m-2s-1. The
photosynthetic performance was measured in both well fed M. pulex cultures and in cultures
which had almost depleted their prey. In the first case, M. pulex cells was separated from its prey
by individually picking the ciliates with a drawn Pasteur pipette and transferring them to 0.2 µm
filtered growth medium. This was repeated 3 times to exclude all H. rotundata. In the second
case, M. pulex cultures were allowed to almost deplete their prey, before bulk measurements of
the mixed cultures were carried out (Table 2). Subsamples were taken for enumeration of prey
and M. pulex cells. This allowed for subtraction of the contribution of H. rotundata to the total
photosynthesis and thus an estimation of the photosynthetic performance due to M. pulex cells.
Photosynthetic rates were measured by a modification of the “single cell method”
(Stoecker et al. 1988, Skovgaard et al. 2000). A NaH14CO3- stock solution (specific activity 100
µCi ml-1) was then added to each vial, containing 2 ml cell suspension of resulting in a specific
activity of ~ 1.0 µCi ml-1. The 2 ml cell suspension contained either 20 or 40 ciliates in the case
where the cells were picked individually, while it was in the range of 400-1300 cells when the
photosynthetic performance was carried out on the mixed cultures (Table 2). The vials were
incubated on a glass shelf with light coming from beneath for 2 h. All measurements were carried
out in triplicates. The vials were always accompanied by parallel dark vials, which were treated
similarly, except that they were wrapped in aluminum foil during incubation.
After incubation, the specific activity of the medium was measured by transferring 100 µl
from each vial to new vials containing 200 µl phenylethylamine. The remaining suspension
received 2.0 ml of 10 % acetic acid in methanol to remove all inorganic C. The vials were dried
Tarangkoon & Hansen
7
overnight at 60 °C and the residue was then re-dissolved in 1.5 ml distilled water. Finally, 10 ml
of Packard Insta-Gel Plus scintillation flour were added to all vials (including those for specific
activity) and activity was measured using a Packard 1500 Tri-Carb liquid scintillation counter.
Calculations of photosynthetic rates were based on Parsons et al. (1984). Total dissolved
inorganic carbon content was measured with a 225-Mk3 infrared gas analyzer (Analytic
Development Co. Ltd. Hoddesdon, England).
Functional and numerical response in the light and in the dark. These experiments were
designed to test the effect of light on growth and ingestion rates of M. pulex. Heterocapsa
rotundata was selected as prey based on the prey selection experiments. Experiments were
initiated by mixing exponentially growing cultures of M. pulex and H. rotundata. Cultures of H.
rotundata were also run as monocultures, thereby allowing the calculation of ingestion rates. All
experiments were carried out in triplicate in 65 ml tissue culture bottles filled to capacity. The
experiments were carried out in darkness (culture bottles were wrapped in aluminum foil), and at
an irradiance of 100 µmol photons m-2 s-1. Irradiance was measured using a LI-COR, LI-1000
radiation sensor equipped with a spherical probe. The initial prey concentrations during these
experiments were approximately 450-18,200 cells ml-1 and a prey:predator cell concentration
ratio of > 10 (based on preliminary data). The initial two days of the incubation served as
acclimation to light and prey concentration. Subsamples, 5-10 ml, were taken for cell counts
every 1-2 days, depending on growth rates, during experimental period (3-6 days) at a fixed time
of day to eliminate potential diurnal variations in ingestion and growth rate of M. pulex.
Subsamples were fixed in Lugol’s (final concentration 1%) and counted on inverted microscope
at 100 x magnification. A Sedgewick-Rafter cell was used for counting when cell densities were
above 200 cells ml-1. Otherwise 2 or sometimes 25 ml sedimentation chambers were used in
cases where cell numbers were lower. To avoid pH effects on the growth of H. rotundata and M.
pulex, pH was monitored during all the experiments with high prey concentrations (above 8000
cells ml-1) directly in bottles. Subsequently, the experimental bottles were refilled to capacity
with fresh filtered f/20 media. In order to keep prey concentrations at a certain concentration and
to avoid pH effects dilution of the experimental bottles and/or addition of prey cells were often
required.
Tarangkoon & Hansen
8
Responses of M. pulex to starvation in the light and in the dark. This experiment was
designed to examine whether or not light affects growth/survival responses of M. pulex when
starved. This experiment was conducted in darkness, 50 and 100 µmol photons m-2 s-1 for 18-21
days. Initial predator-prey concentration ratio was ca 200:2800 cells ml-1. Monocultures of H.
rotundata were also run parallel with all mixed cultures. The experiments were adapted to each
irradiance level for 2 days (Day 0-2). Subsamples (5-10ml) were collected at Day 2, 3, 4 and
thereafter every 2 days until the termination of each experiment and the experimental bottles
were filled to capacity with fresh filtered f/20 media. The mortality rate was calculated in the
same way as growth (see previous section). For comparison between light treatments, t-test
analyses were used to test for differences between means. Mean values were averaged using only
data after Day 4 (= no residual prey in all treatments).
RESULTS
Feeding and growth of M. pulex fed different prey items
The culture of M. pulex subjected to starvation decreased in cell concentration throughout the
duration of the experiments and estimated mortality rates were in the range to 0.85-1.07 d-1 after
Day 2 (Fig. 1A, 2A). In the experiment where M. pulex was fed H. rotundata at an initial prey
concentration of 1000 cells ml-1, the ciliate almost depleted the prey during the first 2 days of the
incubation and the negative growth of the ciliate was observed (-0.02 d-1) (Fig. 1B). Additional
prey was therefore added on Day 2, thereby increasing the H. rotundata cell concentration to
10,000 cells ml-1. On Day 4, the prey populations had been considerably reduced, and M. pulex
had grown with an average rate of 0.78 d-1 during this period (Fig 2A). Additional prey was not
added on Day 4, which lead to a total depletion of prey on Day 6, and a decrease in M. pulex
concentration.
The response of M. pulex to the cryptophytes was quite different than to that of H.
rotundata. Mesodinium pulex ingested all of the offered species, however, it was unable to
control the growth of any of them (Fig. 1, 2). Highest ingestion rates were obtained with
Tarangkoon & Hansen
9
Teleaulax sp and Guillardia theta as prey (22-25 and 12-15 cells predator-1 d-1, respectively),
while lower ingestion rates were obtained with the other prey species (2-10 cells predator-1 d-1)
(Fig 2B). Taking the different cell volumes of the individual preys (Table 1), allow for a
calculation of ingested biovolume in each case (Fig. 2C). From this it is evident that the
difference in growth rates on the different preys are reflected in different levels of ingestion rates
(in terms of biovolume).
The highest growth rate of M. pulex in this experiment was obtained on Teleaulax sp.
(0.8-1.0 d-1). However, this growth rate was obtained at a much higher cell concentration than
when M. pulex was fed H. rotundata (Fig.1, 2). Mesodinium pulex did also grow well when fed
G. theta, although growth rates were lower (0.5 d-1). In cases where M. pulex were fed Teleaulax
amphioxeia, H. rufescens or H. tepida, M. pulex either maintained its population (on T.
amphoxeia) or died out during the incubation even though cell concentrations became high (H.
rufescens and H. tepida). Overall, it seems like M. pulex needs to ingest a biovolume of >800-
1000 µm3 d-1 to support growth.
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Fig. 1. Cumulative growth (corrected for dilutions) of M. pulex when fed on different prey: (A) no prey,
(B) H. rotundata, (C) H. rufescens, (D)H. tepida, (E) G. theta, (F) Teleaulax sp., (G) T. amphioxeia. Dash
line indicates addition of prey and arrow indicates half volume dilution. Dot line represents the speculated
of died out H.rotundata. Symbols represent treatment means± 1SE
Tarangkoon & Hansen
11
Fig. 2. Mesodinium pulex. (A) Growth rate of M. pulex fed on different prey items (B) Ingestion rate of M. pulex (C) Ingestion rate in terms of biovolume of M. pulex. Black bars represent the measurement data between Day 2 to Day 4. Bars represent treatment means± 1SD.Grey bars represent the measurement data between Day 4 to Day 6. Asterisk indicates that the experiment was ended on Day 4.
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The pH of the medium was monitored in both monocultures and in the mixed cultures throughout
the duration of the experiments (Fig. 3). In the mixed cultures it remained below pH 8.1, except
for in the mixed culture with Teleaulax sp. as prey. Here it increased to pH 8.6 at the end of the
experiment. In the algal monoculture, pH increased to reach high pH values in the cases of
Teleaulax sp. and Heterocapsa rotundata (pH 9). This coincides with the entry into stationary
growth observed in the monocultures of these species between Day 4 and 6 (Fig.1 B, F).
Photosynthetic performance of Mesodinium pulex and the prey Heterocapsa rotundata
The photosynthetic performance of Mesodinium pulex was 2.3 ± 0.86pg C cell-1 h-1 (average ±
SEM) when fed the dinoflagellate Heterocapsa rotundata in excess at an irradiance of 100 µmol
photons m-2s-1 (Table 2). This is equivalent to a daily rate of ca. 32 pg C cell-1 (14:10 light dark
period). In M. pulex cultures that have just about depleted the prey in mixed cultures that
Fig. 3. pH dynamics during the experiment of M. pulex fed different prey types (A) monocultures of prey items ,(B) monocultures of M. pulex and mixed cultures of prey and predator. Symbols represent treatment means ± 1SE.
Tarangkoon & Hansen
13
photosynthetic performance was much lower, 0.37 ± 0.08 pg C cell-1 h-1 (average ± SEM) (Table
2). This is equivalent to ca. 5.2 pg C cell-1 d-1. For comparison, the photosynthetic performance of
H. rotundata cells in monocultures were 3.76 ± 0.18 pg C cell-1 h-1 or 52 ± 2.7 pg C cell-1 d-1
(average ± SEM, n=21).
Table 2. Photosynthetic performance of well fed and starved M. pulex cells when fed H.rotundata as prey.
n = number of replicates.
Functional and numerical responses in the dark and in the light
The functional response of M. pulex fed H. rotundata was investigated in the light (100 μmol
photons m-2 s-1) and in the dark (Fig. 4A). Maximum ingestion rate was 49 H. rotundata cells
predator-1 d-1 in the light, while it in the dark the maximum ingestion rate was considerably
lower, 27 H. rotundata cells predator-1 d-1 (t-test, p<0.01). Half saturation constants were 1210
and 1580 cells ml-1 for the light and dark treatments. Ingestion rates of ~35 and 15 H. rotundata
cells predator-1 d-1 were sufficient to maintain maximum growth rate of M. pulex in the light and
in the dark, respectively (Fig. 4A).
The growth rate of M. pulex fed H. rotundata followed Michaelis-Menten kinetics both in
light and in the dark (Fig. 4B). In the light the growth rate of M. pulex reached the maximum
growth rate of 1.41±0.03 d-1 at average prey concentration of ~6,700 cells ml-1. A prey
concentration of ~40 cells ml-1 was required for maintenance (μ=0). In the dark M. pulex reached
a maximum growth rate of 1.19±0.07 d-1, which was significantly (t-test, p<0.01) lower than the
Range in average ciliate Range in average prey Photosynthetic rate
Physiological state concentrations concentrations average±SEM n
(cells ml-1) (cells ml-1) (pgC cell-1h-1)
Starving cells 210-645 4-83 0.37±0.08 21
Well fed cells 131-441 3090-18266 2.32±0.86 12
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maximum growth rate obtained in the light (Fig 4B). Maintenance (μ=0) in the dark was obtained
at a prey concentration of ca.10 cells ml-1, which was also lower than that found in the light.
Response of M. pulex to starvation in the dark and at two light levels
The effect of starvation on survival and cell volume of M. pulex when exposed to
irradiances of 100 and 50 μmol photons m-2 s-1 and to darkness was studied (Fig. 5). During
acclimation period (Day 0-2), prey concentrations were high in all cases leading to initial high
growth rates of M. pulex (Fig.5D). Prey was depleted at Day 3 and 4 in the light and in the dark,
respectively, and M. pulex subjected to sudden starvation. Initial growth rates were 0.99, 0.95 and
0.71 d-1 (Day 0-2) for the light treatments (100 and 50 μmol photons m-2 s-1) and the dark,
respectively. These initial growth rates changed to mortality rates of 0.55, 0.62 and 0.09 d-1 for
the light treatments (100 and 50 μmol photons m-2 s-1) and the dark, respectively during Day 4-6
(Fig. 5D). After Day 6, the mortality rates stayed fairly constant over the next 10-14 days, maybe
with the exception of a slightly increased mortality rate from 0.46 to 0.73 d-1 during Day 12-16 in
the light treatments. From Day 6, the mortality rates of the light treatments were significantly
higher than the dark (t-test, p<0.05). No different of the mortality rates between high light and
medium light showed in the same period (t-test, p>0.05).
Tarangkoon & Hansen
15
The cell volumes of M. pulex decreased slightly from Day 2 to Day 6 of the starvation
experiment in all treatments (Fig 5E). After Day 6 the cell volumes were constant. The observed
changes were not significantly different among treatments (t-test, p>0.05).
Growth/survival experiments were also carried out on monocultures of H. rotundata in
the 2 light levels and dark to follow the fate of the prey organisms in three treatments (Fig 5A-C).
In the high and medium light treatments, H. rotundata grew at a rate of 1.11 and 0.90 d-1,
respectively for the first 6 days of the incubation. In the dark treatment, H. rotundata maintained
its cell concentration for the first 4 days of the incubation. However, at Day 6 no cells were left
any more.
Fig. 4. Mesodinium pulex. (A) Ingestion rate of M . pulex fed on H.rotundata at 2 irradiances (100 and 0 μmol photons m-2 s-1) as function of prey concentration (Cp), (B) Growth rate (μ) of M. pulex when fed on H. rotundata at 2 irradiances. Symbols represent treatment means± 1SE. The curves were fitted to Michaelis-Menten kinetics.μ = 1.44 x (Cp- 40)/(707.13+( Cp-40) for the high light and μ = 1.10 x (Cp- 10)/(1745.44+( Cp-10) for the dark incubated
Tarangkoon & Hansen
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Fig. 5. Mesodinium pulex. Cumulative growth (corrected for dilutions) of M. pulex at (A)100 μmol photon m-2 s-1, (B) 50 μmol photon m-2 s-1, (C) Dark, (D) Growth rate of M. pulex at 3 different irradiances, (E) Cell volume of M. pulex at different irradiances. Symbols represent treatment means± 1SE. Dot line represents the speculated of died out H. rotundata from the monoculture.
Tarangkoon & Hansen
17
DISCUSSION
Prey selection in Mesodinium pulex
All Mesodinium species are phagotrophic and they seem to rely on motile prey for food (i.e.
Dolan & Coats 1991, Gustafson et al. 2000, Jakobsen et al. 2006). The motile prey is detected by
a band of equatorial cirri encircling the cell by sensing hydrodynamic disturbances of the medium
made by the swimming prey. Prey capture in M. pulex has been studied in some detail (Jakobsen
et al. 2006). Upon detection Mesodinium pulex immobilizes the prey cell using the oral tentacles,
which have extrusomes attached to each tentacle tip (Lindholm et al. 1988, Dolan&Coats 1991,
Tamar 1992, Jakobsen et al. 2006). The prey is then transported to the mouth by retraction of the
tentacles (Jakobsen et al. 2006). The entire process takes typically less than 1/30 of a second from
detection to ingestion of H. rotundata (Jakobsen et al. 2006).
In our study, M. pulex successfully ingested all the offered prey organisms, which were in
the size range of 4.7-6.3 µm (estimated spherical diameter, ESD). However, the ingestion rates
varied in terms of both cells-1 h-1 and as biovolume h-1, allowing it only to grow when fed 2 out of
the 5 different preys offered. Previously, it has been shown that M. pulex cannot ingest algae like
the haptophyte Phaeocystis globosa (4 µm, single cells) or the cryptophyte Rhodomonas salina
(ESD 7 µm) (Tang et al. 2001, Jakobsen et al. 2006). Also, quite low ingestion rates were
obtained with the dinoflagellate Gymnodinium simplex (ESD 9 µM) as prey. This raises the
question of what causes this differentiated ingestion rate of quite similarly-sized prey cells.
Jakobsen et al. (2006) showed that fast swimming prey like the dinoflagellate Gymnodinium
simplex will make deformation rates in the water of a magnitude that disguises its as a predator
and the ciliate performs jumps upon encountering it in the same way as it does when escaping an
approaching predator. None of the cryptophytes studied in the present investigation were
particularly fast swimming cells, so that explanation can be ruled out. However, since M. pulex
feeds rheotactically on motile prey, swimming prey cells need to generate a sufficient
deformation rate to trigger an attack from M. pulex. That is; slow swimming among prey cells
may be a strategy aimed at reducing detection. This leaves us with two possible mechanisms that
can account for the low capture success of M. pulex on the cryptophytes. Either the ciliate is
Tarangkoon & Hansen
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unable to physically capture the prey cells or cells are able to a large extent to evade being caught
by jumping away from the ciliate or by reducing its deformation rate due to slow swimming.
Physical capture of prey cells in M. pulex involves 3 overall steps: Prey immobilizing
using the extrusomes, followed by adhesion of the prey to the tentacle tips, and finally ingestion
of the prey (Jakobsen et al. 2006). At each of the capture steps there is chance that M. pulex will
fail to ingest the prey. Jakobsen et al. (2006) demonstrated that M. pulex paralyzed the
cryptophyte R. salina less efficiently than it did the dinoflagellate H. rotundata (Jakobsen et al.
2006), indicating that the initial attack is an important step in the capture success of M. pulex.
However, attaching prey to the tentacles may involve a complex and often highly specific
recognition process between prey surface lectines and glycoprotein binding sites on the predator
that acts as prey discriminating component in itself as has been shown in other protists
(Sakaguchi et al. 2002, Wotton et al. 2007). Our visual investigation in the inverted microscope
did not suggest that the cryptophytes we offered to M. pulex could escape by jumping away from
the ciliate upon attack. Hence, the low capture success is most likely a product of both the
inability of the extrusomes to properly attack to the prey and an inefficient predator prey match of
binding glycoproteins and prey lectines.
If this explanation is true, why can not the low capture success be compensated for by
high concentrations of prey cells? Even though cryptophyte concentrations were very high in
some cases, ingestion rates were still low. The reasons for this lie most probably in the
Mesodinium spp capture mechanism. Mesodinium spp use tentacles equipped with extrusomes
(Lindholm et al.1988). Extrusomes can most likely only be fired once, where after they will have
to be replaced. In Mesodinium spp. this probably means that the tentacles will have to be
withdrawn into the cell and reabsorbed when the extrusomes have been fired, and new ones
synthesized. This will put a maximum limit to the number of capture attempts by the ciliate and
may thus explain our results.
Tarangkoon & Hansen
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Effects of light on growth and ingestion by Mesodinium pulex
Mesodinium pulex displayed considerably higher growth rates in the light (using a 14:10
light:dark cycle) compared to the dark, irrespective of prey concentration (Fig 4). This may
suggest that it is mixotrophic and that it gains a considerable input in terms of carbon from
photosynthesis. Rates of photosynthesis of M. pulex were ~ 32 pg C cell-1 d-1 in well-fed cultures,
which is about ten times lower than the rates measured in the predominantly photosynthetic
mixotroph Mesodinium rubrum using the same techniques (Hansen & Fenchel 2006). Using a
growth efficiency of the carbon uptake due to photosynthesis of 50 % in phototrophs (Hansen et
al. 2000), suggests that 16 pg C will be available for M. pulex growth per day. For comparison, a
M. pulex cell contains ~210 pg C (Table 3). The maximum growth rate of M. pulex in the light
was ~ 1.4 d-1 or 2 doublings per day (Fig. 4B). This means that the expected growth increase due
to photosynthesis of a well-fed M. pulex culture is less than 4 %. Thus, the increased growth rates
in M. pulex in the light, were by and large due to increased ingestion rates in the light (Fig. 4).
Table 3. Cell volumes, estimated spherical diameter and estimated cell carbon contents of M. pulex (well
fed) and H. rotundata cultured in f/20 medium at an irradiance of 100 μmol photons m-2 s-1 and in the dark
(n=20). Carbon content was estimated using the equations given by Menden-Deuer & Lessard (2000).
The finding of increased ingestion and growth rates in M. pulex in the light is not unique
among heterotrophic protists, although very little information is presently available. Elevated
ingestion and growth rates in the light compared to in the dark (or very low irradiance levels; i.e.
≤ 20 µmol photons m-2s-1) have previously been shown for a heterotrophic dinoflagellate,
Irradiance
(μmol photons m-2 s-1) Species
Cell volume±SD
(µm3) ESD(µm)
Estimated Carbon content
±SD (pg C cell-1)
100 Mesodinium pulex 1510.93±445.22 13.7 208.30±57.93
Heterocapsa rotundata 116.94±41.55 5.9 37.21±2.43
Dark Mesodinium pulex 1431.93±413.46 13.5 198.12±53.59
Heterocapsa rotundata 89.44±21.35 5.4 29.96±7.37
Tarangkoon & Hansen
20
Gymnodinium sp (Skovgaard 1998) and 2 ciliates: Coxliella sp. and Strombidinopsis acumicatum
(Strom 2001).
Effects of light on ingestion and growth can be due to both direct effects of light on
digestion rates and to indirect effects due to light effects on food quality. Our growth and grazing
experiments were not designed to differentiate between direct and indirect light effects, such as
prey quality, or prey carbon content etc. However, Strom (2001) found direct positive effect of
light on ingestion rates of 2 ciliate species, which increased by a factor of 2-7 at all irradiances
≥20 µmol photons m-2s-1 using short term incubations (mins) and FLAs (fluorescently labeled
algae) as prey. Thus, although very few data are available on light effects on ingestion and
growth of heterotrophic ciliates, these data tend to suggest that our observations of increased
ingestion and growth rates in the light at least partly can be explained by direct light effects on
ingestion rates. What causes the increased ingestion rates in the light? Previous studies on the
topic have suggested “light aided” digestion of prey (Skovgaard 1998, Strom 2001). This is not
unlikely because it is well established that dissolved organic matter is photochemically
degradable (reviewed by Moran & Zepp 1997). No data are however available on the effect of
light on digestion rates in heterotrophic protists, apart from Strom (2001), who found a 40-fold
increase in loss of food vacuoles (a proxy for digestion rate) of the heterotrophic dinoflagellate
Noctiluca scintillans, in the light compared to in the dark, when fed algal prey. More research on
this topic is required to see if this might be a general phenomenon among heterotrophic protists.
Survival response in the light and in the dark
Mesodinium pulex cultures, which were allowed to deplete their prey to very low cell
concentrations, displayed very low rates of photosynthesis (Table 2). Also, when M. pulex was
subjected to starvation, it survived better in the dark than in the two light treatments in our
experiments. Thus, we can reject our hypothesis that photosynthesis in the light slows down
mortalities compared to in the dark. Previous studies of survival responses of algivorous
heterotrophic protists (2 ciliates and 1 dinoflagellate) have not been able to demonstrate any
differences between light and dark treatments (Skovgaard 1998, Strom 2001). So why does M.
pulex differ so much from the other species tested?
Tarangkoon & Hansen
21
Mesodinium pulex is known to ingest preys of its own size (i.e. the ciliate Metanophrys
sp., Dolan & Coats 1991) and has very recently been shown to be cannibalistic (Moestrup et al. in
prep). None of the species tested by Skovgaard (1998) and Strom (2001) have been reported to be
cannibalistic. Thus, if M. pulex indeed eats its own kind when starved and if ingestion and
digestion rates in the light are higher compared to those in the dark this may explain the higher
mortality rates observed in the light. This question would be worth studying in much more detail.
CONCLUDING REMARKS
Many different groups of protists capture single prey items using a variety of capture
mechanisms. Dinoflagellates often use capture filaments, while haptophytes often use the
haptonema for prey capture (Kawachi & Inouye 1995, Hansen & Calado 1999). The cyclotrichs,
including Mesodinium spp, use extrusomes for prey capture. Our knowledge on the mechanisms
of prey selection in many protist predators, which rely in single prey capture, is very limited.
While some studies indicate chemical as well as mechanical cues for detection of prey items
(Jacobson & Anderson 1986, Hansen & Calado 1999, Naustvoll 2000a,b, Jakobsen et al. 2006,
Riisgaard & Hansen 2009), the role of capture success of such predator types is largely
unexplored (e.g. Jakobsen et al. 2006). In Mesodinium pulex, we show that prey species with a
similar size are ingested at very different rates, suggesting that the cell surface of the prey is
important in avoiding being consumed by predators like Mesodinium. Much more work is needed
on this topic and its significance in species succession, prey/predator population dynamics and
carbon flow in marine waters.
An unexpected result of our work was the apparent role of light for ingestion and growth
of M. pulex. Our hypothesis of a significant role of photosynthesis for the growth and survival of
M. pulex was rejected. Thus, our data instead support the suggestions by Skovgaard (1998) and
Strom (2001) that light plays a direct role in the digestion of prey by heterotrophic protists. Most
studies dealing with the growth and grazing rates of heterotrophic ciliates have been carried out
in complete darkness or at very low levels of irradiance (< 25 µmol photons m-2s-1; Verity 1991,
Montagnes et al. 1996, Jeong et al.1999, Gismervik 2005). The reasons for the incubation of such
experiments at low or no light among most researchers have primarily been to reduce prey
Tarangkoon & Hansen
22
growth rate during the experiments and the fact that light has not been considered an important
factor for grazing of entirely heterotrophic protists. If light turns out to play an important role for
digestion of prey by protists in general it may have considerable implications for the application
of ingestion data from laboratory experiments to field abundances of protists aiming to estimate
the role of protists in microbial food webs (e.g. Hansen et al. 1997). Thus, work on this topic is
strongly urged.
Acknowledgements
We are indebted to Hans Henrik Jakobsen, Øjvind Moestrup and Lydia Garcia-Cuetos for
comments and suggestions, which improved this paper significantly. We thank Hans Henrik
Jakobsen for the use of this isolate of Mesodinium pulex. This study was supported by the Danish
Research Council to Per Juel Hansen, grant no 272-06-0485, and a PhD grant from Rajamangala
University of Technology Srivijaya, Thailand to Woraporn Tarangkoon.
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PAPER III
Does the marine red tide ciliate Mesodinium rubrum
have replaceable symbionts?
Hansen PJ1*, Moldrup M1, Tarangkoon W1,3, Garcia-Cuetos L2, Moestrup Ø2
1Marine Biological Laboratory, Department of Biology, University of Copenhagen, Strandpromenaden 5, DK-3000 Helsingør 2Phycology Laboratory, Department of Biology, University of Copenhagen, Øster Farimagsgade 2D, DK-1353 Copenhagen 3Faculty of Science and Fisheries Technology, Rajamangala University of Technology Srivijaya, 92150 Trang, Thailand
*Corresponding author: [email protected]
KEY WORDS: Mesodinium rubrum, symbionts, ingestion, cryptophytes
ABSTRACT:
The red tide ciliate Mesodinium rubrum (=Myrionecta rubra) is known to contain a symbiont of
cryptophyte origin. Molecular data have shown that the symbiont is very closely related or similar
to free-living species belonging to the “Teleaulax clade”. This suggests that the symbiont of M.
rubrum is either a temporary symbiont or a quite recently established symbiont. Here we present
data from a number of experiments in which we tried to replace the symbionts in M. rubrum by
supplying a number of different cryptophyte species belonging to different cryptophyte clades.
Growth and ingestion rates of M. rubrum fed these cryptophytes were measured. In addition, cells
of M. rubrum were analyzed for type of chloroplast using transmission electron microscopy and
DNA sequences of the nucleomorph LSU. We found that M. rubrum ingested all the offered
cryptophyte species, but it was unable to incorporate any of the offered prey species as
symbionts. Also, M. rubrum can only sustain growth on cryptophyte species belonging to the
“Teleaulax clade”.
Hansen et al.
2
INTRODUCTION
Mesodinium rubrum is a ciliate which hosts a cryptophyte symbiont including cryptophyte
chloroplasts, mitochondria, nucleomorph, cytoplasm and a so-called “symbiont nucleus” (Taylor
et al. 1971, Hibberd 1977, Hansen & Fenchel 2006). Several isolates are now in culture from
Antarctic (Gustafson et al. 2000) and temperate waters (e.g. Yih et al. 2004, Hansen & Fenchel
2006). To what extent they belong to the same species is presently unknown as some
morphological and physiological differences exist between them. For example the Danish and the
Antarctic strains differ in terms of chloroplast number and the occurrence and size of the
symbiont nucleus. The Danish isolate has on average about 17-20 chloroplasts per cell (Hansen &
Fenchel 2006), while only about 8 chloroplasts have been found in the Antarctic isolate
(Gustafson et al. 2000, Johnson & Stoecker 2005, Johnson et al. 2006). Also, while the Danish
isolate apparently has a permanent large symbiont nucleus (Hansen & Fenchel 2006), the
Antarctic isolate may lose its symbiont nucleus but acquire it again (Johnson et al. 2007). Also,
the nucleus of the Antarctic isolate seems to be able to grow in size. This suggests that the strains
belong to different species, but it is currently unresolved.
It has previously been shown that the Danish isolate of M. rubrum only needs to ingest 1
cryptophyte cell per day to grow at its maximum growth rate (Smith & Hansen 2007). In term of
ingested carbon, the food uptake only accounts 1-2 % of the carbon requirements per day for M.
rubrum. This raises the question why M. rubrum has to eat to sustain growth? One explanation
could be that M. rubrum harbors chloroplasts of its own but needs to ingest prey to obtain some
essential micronutrients. Another alternative could be a renewal of the chloroplast after it
underwent several divisions within the host. This implies that the chloroplasts are replaceable
and have to be acquired from prey cells for sustained growth. So far, the Korean and the Danish
isolates have been cultured on Teleaulax spp, while the Antarctic isolate has been cultured on
Geminigera cryophila. All these cryptophytes are closely related and belong to the same
cryptophyte clade ( Hoef-Emden, 2008) Recently however, Park et al. (2007), found some growth
of the Korean M. rubrum when fed a cryptophyte belonging to the “Rhodomonas” clade
suggesting that M. rubrum might be able to utilize different types of cryptophytes. However, to
what extent, M. rubrum can ingest and grow on a large variety of different kinds of cryptophytes
is presently unknown.
Hansen et al.
3
The aim of present investigation was to study ingestion and growth responses of the
Danish isolate of M. rubrum when offered 7 different species of cryptophytes belonging to
different marine cryptophyte clades and 1 dinoflagellate species. Moreover, we tried to determine
to what extent M. rubrum can replace its symbionts using ultrastructural and molecular
techniques
MATERIALS AND METHODS
Cultures. A culture of the photosynthetic ciliate Mesodinium rubrum was established from
single cells isolated from surface sea water samples collected in Frederikssund, Denmark, during
a bloom event on April 17th 2007. Single cells were isolated from the samples using a drawn-out
Pasteur micropipette. The isolates were washed by several transfers through sterile-filtered
seawater (0.20 μm Satorius Minisart filter) before placing in multi-dish wells (3 cells per well),
each containing 2 ml sterile filtered seawater-based h/20 (Guillard 1975) medium with a salinity
of 30. The cryptophyte Teleaulax amphioxeia (K-0434) was used as prey species for M. rubrum
and was established from seawater samples collected from the Øresund in March 1990,
Denmark, and provided by the Scandinavian Culture Collection of Algae and Protozoa of the
University of Copenhagen. Every 2-3 d, 1 drop of dilute T. amphioxeia suspension in sterile
filtered h/20 medium was added to the wells containing M. rubrum. When M. rubrum was not
able to consume all added T. amphioxeia, it was transferred to a new vial containing new sterile-
filtered h/20 medium. After one month the wells contained dense suspensions of M. rubrum. All
cultures were kept in h/20 medium at a salinity of 30 and kept on a glass table. Light (cool white,
100 µmol photons m-2 s-1) was provided from beneath in a light:dark cycle of 14:10. All
experiments were performed at a temperature of 20±1°C.
Hansen et al.
4
Growth and survival responses of Mesodinium rubrum when fed 7 different cryptophytes
and a dinoflagellate.
Seven cryptophyte species and 1 dinoflagellate were tested as food for Mesodinium rubrum
(Table 1). The selection covers the 4 marine clades found within the cryptophytes (Fig. 1).
Information on the strains is listed in Table 1. All experiments were carried out in 65-ml tissue
culture bottles filled to capacity. Samples (2ml) were withdrawn at Day 4, 8, 12 (or 10) and
bottles were refilled to capacity with fresh h/20 medium. It has previously been shown that
growth of M. rubrum is affected when pH exceeds 8.5 and that it dies when pH exceeds 8.8
(Smith & Hansen 2007). Thus, in order to interpret data correctly, pH was measured directly in
the bottles on each sampling occasion, using a Sentron Argus pH meter equipped with a HOT-Fet
line pH probe, which was calibrated using standard buffers of pH 7 and 10. Prior to all
experiments, M. rubrum was grown on the cryptophyte T. amphioxeia. Only M. rubrum cultures
that had just depleted the prey were used for experiments.
Table 1. Protist strains used as prey in the experiments.
Species Culture collection - ID number GenBank accession number Heterocapsa rotundata K-0483 (SCCAP) NA Chroomonas vectensis CCMP-432 HM126534 Guillardia theta CCMP-2712 X57162 Hanusia phi CCMP-325 U53126 Hemiselmis tepida CCMP-442 HM126533 Proteomonas sulcata CCMP-321 HM126536 Rhodomonas salina K-1487 (SCCAP) HM126532 Teleaulax amphioxeia K-0434 (SCCAP) AJ421146
In the first set of experiments, the two cryptophytes T. amphioxeia and Proteomonas
sulcata and the dinoflagellate Heterocapsa rotundata were used as prey for M. rubrum. Initial
cell concentrations were 1000 cells ml-1 of both prey and predator. This prey concentration was
chosen based on the previous observation that maximum growth of M. rubrum occurs during
these conditions when fed T. amphioxeia. Moreover, it has been shown that M. rubrum ingests ~1
prey cell (T. amphioxeia) d-1 at this prey concentration (Smith & Hansen 2007), which means that
M. rubrum should be able to control its prey if it ingests the offered prey. In cases where prey was
depleted totally in the experimental bottles, M. rubrum from that particular experiment was
subcultured (i.e. diluted) and prey concentrations were increased and/or sampling frequency
Hansen et al.
5
increased. Control experiments were carried out with both a monoculture of unfed M. rubrum and
monocultures of the given prey species. All experiments were carried out in triplicate.
In the second set of experiments, the growth responses of M. rubrum were studied when
offered five species of cryptophytes: Chroomonas vectensis, Rhodomonas salina, Hanusia phi,
Guillardia theta, Hemiselmis tepida. The setup of the experiment was as in the first set of
experiments, except that all the mixed cultures were diluted on Day 4 to diminish effects of
elevated pH on the outcome of the experiments.
Measurements of ingestion rates
Ingestion rates of M. rubrum were determined from the decrease in prey concentrations over 2-4
day periods when comparing with the growth of the control cultures as described by Jakobsen &
Hansen (1997). The ingestion rate U was estimated using the following 2 equations:
Uyµdtdx
x −= (2)
yµdtdy
y= (3)
where (x) is ingested by grazer (y). It is assumed that the grazer (y) grows exponentially with the
rate constant of μy and that the prey (x) grows with the rate constant of μx. The mortality of the
cryptophytes due to grazing is Uy, where U (cells predator-1d-1) is the per capita ingestion rate,
which is independent of x. The ingestion rate (U) was iteratively calculated using “Prey” (by B.
Vismann) software (Jakobsen & Hansen 1997).
Hansen et al.
6
Fig.1. Phylogeny based on nuclear SSU rDNA sequences (1572 bp) inferred from Bayesian analysis. Glaucocystis nostochinearum, Gloeochaete wittrockiana, Cyanophora paradoxa and Cyanoptyche gloeocystis constituted the outgroup. Branch support was obtained from Bayesian posterior probabilities and bootstrap (100 replicates) in maximum likelihood analyses. At internodes, posterior probabilities (1) are written first followed by bootstrap values (in percentage) from ML. (*) Highest possible posterior probability (1.0) and bootstrap value (100%). Species in bold face were used for the experiments in this study.
Hansen et al.
7
Transmission electron microscopy (TEM)
A culture of Mesodium rubrum fed Hemiselmis tepida was mixed 1:1 with 4% glutaraldehyde in
0.2 M cacodylate buffer at pH 7.4 and containing 0.4 M sucrose. After 1 hour at 4 ºC, the cells
were concentrated by centrifugation. Subsequently, they were rinsed 3 times in cold cacodylate
buffer of decreasing sucrose content. Once rinsed, the material was post-fixed overnight in 2%
osmium tetroxide in 0.2 M cacodylate buffer at pH 7.4 at 4 ºC. Before dehydration, the material
was rinsed briefly in buffer. Each step of the dehydration lasted 20 min at 4 ºC in the following
ethanol concentrations: 30%, 50%, 70%, 90% and 96%. The material was transferred to room
temperature while in 96% ethanol and dehydration completed in two changes of absolute ethanol,
20 min in each change. Following two brief rinses in propylene oxide, the material was
transferred to a 1:1 mixture of Spurr’s embedding mixture (Spurr) and propylene oxide and left
uncovered overnight, followed by 5 hours in a fresh mixture of Spurr. The material was then
moved to a new recipient and Spurr was added. Finally, it was polymerized at 70 ºC overnight.
Sectioning was carried out on a Reichert Ultracut E ultramicrotome using a diamond knife. The
sections were collected on slot grids (Rowley & Moran 1975) and stained for 15 min with 2%
uranyl acetate in methanol, followed by Reynold’s lead citrate. The grids were examined in a
JEM-1010 electron microscope (JEOL, Tokyo, Japan), fitted with a digital camera.
DNA extraction, cell isolation, PCR amplification, cloning and sequencing
The DNA extractions of the cryptophytes were performed as previously described in Hansen et
al. (2003) on the cryptophyte species used as prey during the present experiment (Table 1).
Samples from the experimental flasks were fixed in acid Lugol at Day 4 for the experiments
carried out with C. vectensis, G. theta, H. phi and H. tepida as well as Day12 for the two latter
species. Lugol-fixed cells of M. rubrum were isolated using drawn Pasteur glass pipette under an
Olympus inverted microscope CKX31 (Olympus, Tokyo, Japan) and washed at least three times
in ddH2O under the inverted microscope to avoid any cryptophyte present in the fixed sample to
be carried with the cell of interest. Finally, the five washed M. rubrum cells were transferred into
a 0.2 ml PCR tube (StarLab, Ahrensburg, Germany) and kept frozen at -20 ºC until further
processing.
Hansen et al.
8
PCR reactions were carried out in 50 µl volume. PCR amplifications of the nuclear SSU
rDNA (nSSU rDNA) of the cryptophytes were performed as outlined in Hoef-Emden et al. 2002,
with a combination of primers CrN1F and SSUBR . While, PCR amplifications of the
nucleomorph LSU rDNA (nmLSU rDNA) were carried out as described in Garcia-Cuetos et al.
(2010) with the primer combination nmLSUCr3F and D3B (Nunn et al. 1996). Prior to
amplification, physical disruption was conducted using glass beads (Sigma-Aldrich, Gillingham,
UK) to ensure cell disruption of the M. rubrum cells (Frommlet & Iglesias-Rodríguez 2008). A
semi-nested PCR amplification was subsequently performed using nmLSUCr3F and D2C
(Scholin et al. 1994) applying the same PCR profile. All PCR reactions were carried out on a MJ
Research PTC-200 Peltier Thermal Cycler (MJ Research Inc, Waltham, MA, USA).
To discriminate between possible copies of the nucleomorph LSU present in M. rubrum,
all gene amplifications were cloned with the TOPO TA Cloning Kit (Catalogue nr. K4500-01)
from Invitrogen (Carlsbad, CA). Following plating, transformed clones were selected and the
nucleomorph LSU was amplified, as described above.
All DNA fragments were purified using Nucleofast, following the manufacturer’s
recommendations (Macherry-Nagel Inc., Bethlehem, Pennsylvania, USA). 500 ng PCR product
was air-dried over night and sent to the sequencing service at Macrogen (Seoul, Korea) for
determination in both directions using the same primers employed for amplification.
Alignments and phylogenetic analyses
To determine the phylogenetic position of our cryptophyte isolates, a data set with numerous
cryptophyte taxa, consisting of nuclear SSU rDNA sequences was analyzed. The sequences were
first aligned using MAFFT 6.624 (Katoh & Toh 2008) and then improved manually using
BioEdit 7.0.5 sequence alignment software (Hall 1999). The data set was composed of 103
sequences including four glaucocystophyte sequences as outgroup taxa based on previous
phylogenetic studies based on non-coding genes (Bhattacharya et al. 1995, Hoef-Emden 2008).
Hansen et al.
9
A Bayesian method was used to infer phylogeny, using the program MrBayes v.3.2
(Huelsenbeck & Ronquist 2001). Two simultaneous Monte Carlo Markov chains (MCMC; Yang
& Rannala 1997) were run from random trees for a total of 2,000,000 generations (metropolis-
coupled MCMC). One of every 50 trees was sampled. AWTY (Wilgenbusch et al. 2004) was
used to graphically evaluate the extent of the MCMC analysis. After excluding the first sampled
trees categorized as the ‘‘burn-in period’’, a consensus tree was constructed using PAUP*
4.0.b10 software (Swofford 2002) based on 39.000 trees. Then, Modeltest (Posada & Crandall
1998), implemented in the PAUP* 4.0.b10 software (Swofford 2002), identified GTR model as
the best. Using these settings, a tree was reconstructed with the online version of the PhyML
software (Guidon & Gascuel 2003) available on the Montpellier bioinformatics platform at
http://www.atgc-montpellier.fr/phyml using the maximum likelihood (ML) method (Felsenstein
1981). The reliability of internal branches was assessed using the bootstrap method with 100
replicates (Felsenstein 1985).
RESULTS
Growth response and food uptake of Mesodinium rubrum when fed Teleaulax amphioxeia as
prey
Mesodinium rubrum grew initially in the unfed control experiments during the first 4-8 days of
the incubation in both experiments (Figs 2A, 3A, Table 2). When M. rubrum was fed Teleaulax
amphioxeia at a prey:predator ratio of 1:1 the growth of M. rubrum was not significantly different
from the unfed control during the first 4 days of incubation. During these first 4 days the prey was
completely depleted (Fig. 2B). When the M. rubrum culture was re-fed T. amphioxeia at a prey
predator ratio of 5:1 on Day 4, growth increased considerably compared to the control during the
subsequent 4 days of incubation (Fig. 2A), and the prey was again completely depleted (Fig. 2B).
A second re-feeding of the M. rubrumon Day 8, in combination with a shortening of the
incubation period to 2 days, resulted in a further increase in M. rubrum growth rate (Fig. 2A).
However, T. amphioxeia was not completely depleted on Day 10 (Fig. 2B). The growth of T.
amphioxeia in monoculture was also studied (Fig. 2D), allowing the calculation of crude
Hansen et al.
10
0 2 4 6 8 10 12
Cel
l con
cent
ratio
n (c
ells
ml-1
)
101
102
103
104
105
106
T.amphioxeia-mix P.sulcatum-mix H.rotundata-mix
B
0 2 4 6 8 10 12
Cel
l con
cent
ratio
n (c
ells
ml-1
)
103
M. rubrum mono M. rubrum fed T.amphioxeiaM. rubrum fed P.sulcatum M. rubrum fed H.rotundata
A
Time (days)
0 2 4 6 8 10 12
pH
7.0
7.5
8.0
8.5
9.0
9.5
10.0M. rubrum monoM.rubrum/T.amp-mix M.rubrum/P.sul-mix M.rubrum/H. rotun-mix
C
Time (days)
0 2 4 6 8 10 12
Cel
l con
cent
ratio
n (c
ells
ml-1
)
101
102
103
104
105
106
T. amphioxeia-mono Psulcata-mono H.rotundata-mono
D
estimates of average ingestion rates for the periods 0-4 days, 4-8 days and 8-10 days. Calculated
ingestion rates varied from 0.4 to 2.5 prey cells M. rubrum d-1, at average prey concentrations of
200-1000 cells ml-1 (see Table 2 or Figure 4 for details).
Fig. 2. Experiment 1. A. Changes in cell concentrations of M. rubrum as a function of incubation time (day) in monoculture and when grown in mixed cultures with the cryptophytes Teleaulax amphioxeia, Proteomonas sulcata, and the dinoflagellate Heterocapsa rotundata. B. Changes in prey cell concentrations in the mixed cultures. Development of pH in the cultures (C) and changes in prey cell concentrations cells in monocultures (D).
Cell c
once
ntra
tion
(cell
s m
l-1)
pH
Cell c
once
ntra
tion
(cell
s m
l-1)
Time (days) Time (days)
A B
C D
Hansen et al.
11
Table 2. Growth rates (average +/- SE) of all prey species (except T. amphioxeia) and M. rubrum in monocultures and mixed cultures during the first 4 days of the incubations (based on data shown on Fig. 2&3). The growth rate of M. rubrum in monoculture was 0.11±0.04 d-1 in the first set of experiments and 0.31±0.02 d-1 in the second set. The number of replicates was 3 in all experiments (n=3). For all species, prey growth rate in the mixed culture was significantly lower than growth rates in monoculture (t-test, p<0.05). The growth rate of M. rubrum in monoculture was not significantly lower than in the growth rate in mixed cultures.
Growth rate (mean ± std) (µ), d-1 Species Prey
in monoculture Prey
in mixed culture M. rubrum
in mixed culture Experiment 1 Heterocapsa rotundata 1.19±0.03 0.77±0.03 0.08±0.04 Proteomonas sulcata 0.91±0.02 0.84±0.01 0.11±0.04 Experiment 2 Chroomonas vectensis 0.82±0.03 0.60±0.03 0.29±0.05 Guillardia theta 1.00±0.01 0.67±0.01 0.37±0.01 Hanusia phi 0.86±0.03 0.49±0.04 0.38±0.02 Hemiselmis tepida 0.78±0.01 0.33±0.01 0.32±0.01 Rhodomonas salina 1.16±0.05 1.02±0.06 0.28±0.04
Growth responses and food uptake of Mesodinium rubrum when fed other prey items
Mesodinium rubrum could not sustain growth when fed the dinoflagellate Heterocapsa rotundata
and the other cryptophytes species tested (Figs 2A&3A). Initial growth of M. rubrum in the
mixed cultures containing these prey types was observed for the first 4-8 days in all cases, but the
growth rate never exceeded the growth of M. rubrum in monoculture. In some cases, M. rubrum
died in the mixed cultures between Day 4 and Day 12, depending upon prey species (Fig.3A).
The death of M. rubrum always coincided with an increase in pH above 9 (Figs 2C&3C).
In many cases the prey items grew fast in both algal monocultures and in the mixed
cultures (Figs 2D&3 D). pH increased above 9 after 8-12 days of incubation, and growth of the
algae decreased and in some cases stopped. A comparison of the growth response of the prey in
monocultures and in the mixed cultures with M. rubrum during the first 4 days of the incubation,
revealed that prey concentrations in the mixed cultures were always lower than in the
monocultures (Table 2). The lower growth rate found in the mixed culture (Table 2) indicated
that M. rubrum ingested all types of the offered prey items. Calculation of ingestion rates during
Hansen et al.
12
0 2 4 6 8 10 12 14
Cel
l con
cent
ratio
n (c
ells
ml-1
)
102
103
104
M.rubrum, monoM.rubrum fed C.vectensis M.rubrum fed R.salina M.rubrum fed H.phi M.rubrum fed G.theta M.rubrum fed H.tepida
A
Time (days)
0 2 4 6 8 10 12 14
Cel
l con
cent
ratio
n (c
ells
ml-1
)
103
104
105
106
C.vectensis, mixR.salina-mix H.phi-mix G.theta-mix H.tepida-mix
B
Time (days)
0 2 4 6 8 10 12 14
pH
7.5
8.0
8.5
9.0
9.5
10.0
10.5
M.rubrum/C.vect-mix M.rubrum/R.sal-mix M.rubrum/H.phi-mix M.rubrum/G.theta-mix M.rubrum/H.tepida-mix
C
Time (days)
0 2 4 6 8 10 12 14
cell
conc
entr
atio
n (c
ells
ml- 1
)
103
104
105
106
C.vectensis, mono R. salina, mono H. phi, mono G.theta, mono H. tepida, mono
D
the first 4 days of the incubation gave ingestion rates around 0.25-0.8 prey cells M. rubrum d-1 at
average prey concentrations of 3200-15300 cells ml-1 (see Table 3 and Fig. 4 for details). These
are considerable lower ingestion rates than when T. amphioxeia was used as prey at lower
concentrations (Table 3). For all species the estimated ingestion rates were significantly different
from zero (p<0.05). It should be stressed that the prey concentration changed considerably in the
experimental flasks, thus the estimated rates are crude average rates for the 2-4 day incubations,
and should not to be regarded as precise measurements.
Fig. 3. Experiment 2. A. Changes in cell concentrations of M. rubrum as a function of incubation time (day) in monoculture and when grown in mixed cultures with the cryptophytes Chroomonas vectensis, Rhodomonas salina, Hanusia phi, Guillardia theta, Hemiselmis tepida. B. Changes in prey cell concentrations in the mixed cultures. Development of pH in the cultures (C) and changes in prey cell concentrations cells in monocultures (D).
Hansen et al.
13
Table 3. Average prey concentrations and end prey concentrations are also listed. In cases where T. amphioxeia was used as prey item, data are also given for Days 4-8 and 8-10. Initial prey concentrations were around 1000 cells ml-1, exception the experiments involving T. amphioxeia, Day 4 and 8, where initial prey concentrations were 5000 cells ml-1. For all species the estimated ingestion rates were significantly different from zero (p<0.05)
Species
Average prey concentration in mixed culture with M. rubrum during the
experiment (cells ml-1)
End prey concentration (cells ml-1)
Heterocapsa rotundata 6817 22000 Chroomonas vectensis 3590 9500 Guillardia theta 4279 12350 Hanusia phi 3172 7300 Hemiselmis tepida 2536 4600 Proteomonas sulcata 12204 42600 Rhodomonas salina 15264 63700 Teleaulax amphioxeia (Day 0-4) 203 7 Teleaulax amphioxeia (Day 4-8) 287 0 Teleaulax amphioxeia (Day 8-10) 1018 33
Prey type
H. rotun
data
C.vecte
nsis
G. theta
H.phi
H. tepid
a
P.sulca
ta
R. sali
na
T.amp,
day 0
-4
T.amp,
day 4
-8
T.amp d
ay 8-
10
Inge
stio
n ra
te (c
ells
d-1
)
0.0
0.5
1.0
1.5
2.0
2.5
3.0
Fig. 4. Mesodinium rubrum. Estimated average ingestion rates calculated based on data shown in Fig. 2 and 3. Only data for the first 4 days (Day 0-4) of the incubations were used, except for the prey T. amphioxeia where ingestion rates also were estimated for Day 4-8 and Day 8-10.
Hansen et al.
14
Transmission electron microscopy
Figures 5A-F are a series of micrographs obtained from a culture of Mesodinium rubrum fed the
cryptomonad Hemiselmis tepida. The longitudinal section of Mesodinium (Fig. 5A) shows
several chloroplasts in the cell, located in both the oral and the aboral end of the cell.
Collectively we designate these as chromatophores as their origin is different (see below).
Hemiselmis is ingested at the oral end of Mesodinium, which here possesses, in addition to a
small mouth, bands of microtubules and a number of tentacles (Fig. 5B). Hemiselmis cells are
ingested whole (Figs 5C&D), and Fig 5C illustrates a cell in which both the cryptomonad
nucleus, the cryptomonad chloroplasts and the two types of trichocysts (ejectosomes) of
Hemiselmis are visible. One of the two macronuclei of Mesodinium is also visible Fig. 5A, while
the small nucleus in the upper part of the cell is from a prey cell. Details of the two types of
chromatophore in Mesodinium are shown in Figs 5E&F. In one type, the permanent
chromatophore or chloroplast of Mesodinium, the thylakoids are grouped in lamella of three
thylakoids (Fig. 5E). For comparison, Fig. 5F shows a chloroplast from a Hemiselmis cell located
within Mesodinium, demonstrating the 2-thylakoid lamella characteristic of Hemiselmis. In
contrast to the 3-thylakoid lamellae, the 2-thylakoid lamellae of Hemiselmis were often not well
preserved in the sections, indicating that they were being subjected to digestion enzymes.
Molecular result
The nuclear SSU rDNA alignment consisted of 1572 bp. The molecular phylogeny based on this
alignment and inferred from Bayesian analysis yielded the tree topology shown in Fig. 1.
Glaucocystophytes rooted the tree and the cryptophytes were divided in five clades. The first
clade included four genera: Hemiselmis, Chroomonas, Komma and Falcomonas. The second
clade comprised four genera: Rhodomonas, Rhinomonas, Pyrenomonas and Storeatula. The third
clade was formed by two genera: Hanusia and Guillardia. The fourth clade was composed of
Cryptomonas genus only. Finally, the fifth clade included three genera: Teleaulax, Plagioselmis
and Geminigera. Proteomonas sulcata and Falcomonas daucoides were isolated and did not
belong to any clade. Yet, the relationship between the clades was unresolved.
Hansen et al.
15
Fig. 5. Mesodinium rubrum grown in culture and fed Hemiselmis tepida. A. longitudinal section of M. rubrum from the oral end (Oe) to the aboral end (Ae) illustrating the cilia, chloroplasts (Chl), starch grains (St), the macronucleus (ma-N) and the bands of microtubules around the mouth. B. section of the mouth (M) of M. rubrum through the tentacles (T) about to engulf a cell of H. tepida (Hr) showing part of the bands of microtubules (Bm). C. Engulfed cell of H. tepida (arrows) within M. rubrum. Visible organelles are the chloroplast, the small (sTri) and large trichocysts (lTri) and the cryptomonad nucleus (N). D. Chloroplast of M. rubrum (arrowheads) with triplets of thylakoids (t-Thy) next to an engulfed cell of H. tepida (arrows) with a chloroplast showing thylakoids in pairs (p-Thy). E. Detail of the chloroplast of M. rubrum harbouring thylakoids in triplets. F. Detail of the chloroplast within H. tepida, which holds thylakoids in pairs.
Hansen et al.
16
DISCSSION Ingestion and growth responses of Mesodinium rubrum when fed different types of prey
Cultures of Mesodinium rubrum from various parts of the world have only been established by
offering them cryptophytes from the Teleaulax/Geminigera clade as food (Table 4). Recently,
Park et al. (2007) found some growth of a strain of M. rubrum fed the cryptophyte strain (CR-
MAL03) belonging to the “Rhodomonas” clade. However, to what extent M. rubrum can ingest
and grow on a variety of cryptophyte prey is presently unknown. In this study, our M. rubrum
strain ingested all the offered prey items. Estimated ingestion rates were in the range of 0.5 to 1
cell d-1 for all prey types exception T. amphioxeia, where ingestion rates were higher at
comparable cell concentrations (Fig. 2, Table 2). From a previous study we know that this level
of ingestion rate suffices to support good growth of M. rubrum (Smith & Hansen 2007). Yet, M.
rubrum did only grow when T. amphioxeia was offered as food.
Table 4. List of successful M. rubrum cultures and preys that they can be maintained on.
Place of origin of culture Prey reference Antarctica Geminigera cryophila Gustafson et al. 2000, Johnson et
al. 2005, 2006, 2007, Hacket et al. 2009
Denmark Teleaulax amphioxeia, Teleaulax sp.
Hansen & Fenchel 2006, Smith & Hansen 2007, Riisgaard & Hansen 2009
Korea Teleaulax spp, Unidentified strains belong to the Teleaulax clade
Yih et al. 2004, Park et al. 2006, 2007, 2010
Japan Teleaulax amphioxeia Nagai et al. 2008, Nishitani et al. 2008a,b
Can Mesodinium rubrum sequester chloroplasts from cryptophyte prey?
This raises the question of why M. rubrum cannot grow when fed other prey types than Teleaulax
spp. although it ingests all kinds of prey items. One explanation could be that M. rubrum relies on
the replacement (from time to time) of chloroplasts for sustained growth. T. amphioxeia is
characterized by having chloroplasts with thylakoids that are arranged in triplets (like in M.
Hansen et al.
17
rubrum). With the exception of P. sulcata that shares this thylakoid structure, the other
cryptophytes used in the present study have thylakoids arranged in pairs (Cuetos-Garcia 2010).
Thus, if functional chloroplasts had been taken up from these other cryptophytes by M. rubrum,
this would be revealed in TEM sections of M. rubrum cells. However, in the case of M. rubrum
fed H. tepida that we studied in detail, prey cells taken up were found in food vacuoles and often
partly digested. The same holds true when we used molecular methods to test whether functional
chloroplasts from other prey species were taken up by M. rubrum. In all cases investigated, we
only found sequences matching T. amphioxeia within M. rubrum cells and never sequences that
would match that of the other prey fed to M. rubrum. Thus, we found no proof of chloroplast
sequestration by M. rubrum when fed these other types of cryptophytes.
Why is Mesodinium rubrum not selective in its choice of prey?
One of the most striking observations of the present study was that M. rubrum ingested all the
offered prey items, including a dinoflagellate of the same size as the cryptophyte prey. This
appears to be a bad strategy for an organism that seems to rely on the ingestion of specific
cryptophyte prey. Like other Mesodinium species, M. rubrum is a raptorial feeder that senses
individual prey items before it attacks and captures them, using the tentacles at the oral end of the
cell (see Jakobsen et al. 2006). Apparently, the ciliate cannot recognize its prey. As long as the
prey has the right size and it is actively swimming it will be ingested. This in turn explains why
M. rubrum, when the prey concentration is high, is able to ingest much more food that it actually
needs (see Smith & Hansen 2007). Therefore, in waters where Teleaulax spp are present all year
round such as in the Kattegat (Denmark), M. rubrum will always eat enough of the right kind of
prey to sustain its growth despite the fact that it might also eat preys that cannot. This feeding
mechanism might seem inadequate, yet it works.
Conclusions and future perspectives
We found no evidence of chloroplast replacement in our strain of Mesodinium rubrum when
offered cryptophytes from a variety of cryptophyte clades. This might suggest that the
cryptophyte symbiont in M. rubrum is a permanent symbiont that cannot be replaced by prey
Hansen et al.
18
ingestion. It implies that M. rubrum feeds to obtain some important, as yet unknown, growth
factor(s) which it can get only through ingestion of cryptophytes from the Teleaulax/Geminigera
clade. Yet, we can not discard the possibility that M. rubrum can replace its symbionts with other
species from the Teleaulax/Geminigera clade. Unfortunately, the only temperate Teleaulax
species available in culture is T. amphioxeia. Thus, to solve the problem of symbiont replacement
in M. rubrum, a cryptophyte species belonging to the Teleaulax/ Geminigera clade that posseses a
distinctive chloroplast or nucleomorph molecular signature from T. amphioxeia needs to be
established in culture.
Acknowledgements This study was supported by the Danish Research Council to Per Juel Hansen, grant no 272-06-
0485, and a PhD grant from Rajamangala University of Technology Srivijaya, Thailand to
Woraporn Tarangkoon.
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APPENDIX-PAPER IV
Putative N2-fixing heterotrophic bacteria associated with dinoflagellate-cyanobacteria consortia in the low-nitrogen
Indian Ocean
Hanna Farnelid1, Woraporn Tarangkoon2,3, Gert Hansen4, Per Juel Hansen2 and Lasse Riemann1*
1Department of Natural Sciences, Linnaeus University, SE-39182 Kalmar, Sweden 2Marine Biological Laboratory, Strandpromenaden 5, 3000 Helsingør, Denmark 3Faculty of Science and Fisheries Technology, Rajamangala University of Technology Srivijaya, 92150 Trang, Thailand 4Department of Phycology, Ø. Farimagsgade 2D, 1353, Copenhagen, Denmark *Corresponding author. E-mail [email protected]; Tel. (+46)480447334; Fax:
(+46)480447340.
Farnelid et al.
1
ABSTRACT
Heterotrophic dinoflagellates bearing unicellular cyanobacterial symbionts are common
within the order Dinophysiales. However, the ecological role of these symbionts is unclear.
Due to the occurrence of such consortia in oceanic waters characterized by low nitrogen
concentrations, we hypothesized that the symbionts fix gaseous nitrogen (N2). Individual
heterotrophic dinoflagellates containing cyanobacterial symbionts were isolated from the
open Indian Ocean and off Western Australia, and characterized using light microscopy,
transmission electron microscopy (TEM), and nitrogenase (nifH) gene amplification, cloning,
and sequencing. Cyanobacteria, heterotrophic bacteria and eukaryotic algae were recognized
as symbionts of the heterotrophic dinoflagellates. NifH gene sequences were obtained from 23
of 37 (62%) specimens of dinoflagellates (Ornithocercus spp. and Amphisolenia spp.).
Interestingly, only two specimens contained cyanobacterial nifH sequences, while 21
specimens contained nifH genes related to heterotrophic bacteria. Of the 137 nifH sequences
obtained 68% were most similar to Alpha-, Beta- and Gamma-proteobacteria, 8% clustered
with anaerobic bacteria, and 5% were related to second alternative nitrogenases (anfH).
Twelve sequences from five host cells formed a discrete cluster which may represent a not yet
classified nifH Cluster. Eight dinoflagellates contained only one type of nifH sequence (>99%
sequence identity) but overall the putative N2-fixing symbionts did not appear host specific
and mixed assemblages were often found in single host cells. This study provides the first
insights into the nifH diversity of dinoflagellate symbionts and suggests a symbiotic co-
existence of non-diazotrophic cyanobacteria and N2-fixing heterotrophic bacteria in
heterotrophic dinoflagellates.
Key words: symbionts, nitrogen fixation, nifH, heterotrophic bacteria, Ornithocercus,
Amphisolenia, Histioneis, dinoflagellates, Indian Ocean, Galathea 3
Farnelid et al.
2
INTRODUCTION
Cyanobacterial symbionts (cyanobionts) of non-photosynthetic dinophysoids (Dinophyceae)
were first observed more than 100 years ago (Schütt 1895). They are thought to function as
photosynthetic partners in their relationship with the host (Taylor 1982) but although many
different types of cyanobacterial and bacterial symbionts have been described for several
dinophysoid genera (such as Amphisolenia, Histioneis, Ornithocercus and Parahistioneis)
little is known about the identity and diversity of these symbionts. Further, their ecological
significance is essentially unknown.
Recently, we found that symbiont-bearing dinoflagellates were most common in the
photic zone of the Indian Ocean characterized by low nutrients and low phytoplankton
biomass (Tarangkoon et al. 2010). This is consistent with previous observations from the
Indian Ocean (Jyothibabu et al. 2006) and the Red Sea (Gordon et al. 1994). Due to this
distribution it has been suggested that the photosynthetic symbionts are N2-fixers
(diazotrophs), providing their hosts with reduced N (Gordon et al. 1994; Jyothibabu et al.
2006). To date only a single study has demonstrated nitrogenase in a cyanobiont of a
heterotrophic dinoflagellate (Foster et al. 2006a); however, several studies indicate that
cyanobionts may be diazotrophic. For instance, even though most of the 65 cyanobacterial
16S rRNA gene sequences retrieved from individual heterotrophic eukaryotic host cells were
related to Synechococcus, three sequences from two Histioneis sp. hosts were related to the
unicellular N2-fixing cyanobacterium Cyanothece sp. (Foster et al. 2006b). Further, using
fluorescent in situ hybridization putative unicellular diazotrophic cyanobionts associated with
dinoflagellates have been observed in the Mediterranean Sea (Le Moal & Biegala 2009) and
the Southwest Pacific Ocean (Biegala & Raimbault 2008).
In tropical and subtropical waters the ubiquitous filamentous cyanobacterium
Trichodesmium sp. (Capone et al. 1997) and intracellular cyanobacterial symbionts of diatoms
(Rhizolenia-Richelia; Carpenter et al. 1999) were long believed to be solely responsible for
pelagic N2 fixation. However, recent molecular studies targeting the nifH gene, encoding the
iron protein component of the nitrogenase enzyme, have shown that free-living unicellular
cyanobacteria are also abundant and can account for a significant fraction of the N2 fixation
(Montoya et al. 2004; Zehr et al. 2001). Similarly, it has recently been recognized that non-
Farnelid et al.
3
cyanobacterial diazotrophs, mostly heterotrophic bacteria, are widespread in marine waters,
and their ecological function and importance is currently unknown (Farnelid & Riemann
2008).
In the present study we sought to identify potential N2-fixing symbionts of
heterotrophic dinoflagellates in the Indian Ocean. The symbionts were characterized using
light microscopy and transmission electron microscopy (TEM) and nifH genes were amplified
from individual symbiont-bearing dinoflagellate hosts using a nested PCR approach. Our
study points to a hitherto unrecognized importance of heterotrophic bacteria for N acquisition
in cyanobacteria-dinoflagellate consortia in tropical waters.
MATERIALS AND METHODS
Sample collection. Sampling was carried out onboard the Danish Navy surveillance frigate
“F359 Vædderen” during Leg 7 of the 3rd Danish Galathea expedition (October–November
2006). Samples were obtained from 21 stations located across the Indian Ocean and along a
transect perpendicular to Broome in North Western Australia (Fig. 1 in Tarangkoon et al.
2010). For nifH gene analysis samples were obtained at stations BR5 to BR9 (17° 03' S, 120°
49' E; 16° 50' S, 120° 34' E; 16° 26' S, 119° 56' E; 16° 15' S, 119° 38' E; 16° 01' S 119° 19' E) in
the Broome transect. Live plankton samples were collected at each station with a 20 µm mesh
size plankton net as vertical hauls, from about 70 m depth to the surface or from water
samples (30 L) from 10 m and 30 m depths collected by Niskin bottles attached to a
conductivity, temperature and depth profiler rosette. Subsequently, plankton was concentrated
using a 20 µm Nitex mesh size filter. The filters were kept immersed during the filtration to
facilitate the retention of live cells. The concentrated samples were transferred to 100 ml of
filtered seawater from which single cells were isolated using a drawn Pasteur pipette. Cells
for nifH gene analysis were then successively washed in three baths of 2 ml 0.2 µm filtered
seawater, placed individually in a 0.2 ml PCR tube, and immediately frozen at -20oC. The
cells included a range of dinoflagellates species (Table 3), though, no Histioneis species were
obtained.
Farnelid et al.
4
Microscopy. Dinoflagellates were examined shortly after sampling using an Olympus BX51
light microscope fitted with a Soft-Imaging ColorView III digital camera and identified as
described in (Tarangkoon et al. 2010). In total ~100 cells were examined in the study. Seven
cells of O. magnificus and two of O. quadratus were collected at station 5 in the Indian Ocean
transect (29° 35' S, 95° 15' E) and preserved for TEM (Tarangkoon et al. 2010). In the
laboratory, sectioning was done on an Ultracut E ultramicrotome using a diamond knife, and
the sections were collected on slot grids and placed on Formvar film. After staining in uranyl
acetate and lead citrate, sections were examined in a JEOL JEM-1010 electron microscope
operated at 80 kV. Micrographs were taken using a GATAN 792 digital camera.
DNA extraction and nifH amplification. DNA was extracted from individual heterotrophic
dinoflagellates with symbionts using an enzyme/phenol-chloroform protocol (Riemann et al.
2008) and 200 μl SET lysis buffer (20% sucrose, 50 mM EDTA, 50 mM Tris-HCl, pH 8.0).
An extraction without added sample served as a control on the purity of the extraction
chemicals. Seven of the samples were instead of the extraction procedure subjected to three
cycles of freeze/thawing (-80°C for 1 min and 75°C for 1 min constituted one cycle), which
lyses the cells (Sebastian & O'Ryan 2001). To amplify nifH, degenerate primers purified by
high-performance liquid chromatography and polyacrylamide gel electrophoresis (Sigma-
Aldrich; Zani et al. 2000; Zehr & McReynolds 1989) were used according to a nested PCR
protocol (Zehr & Turner 2001) using Pure Taq Ready-To-Go PCR Beads (GE Healthcare). A
negative control reaction with UV-treated water was included in each PCR batch. To
minimize the risk of contamination, mixing of reagents was done in a UV-treated sterile flow
bench in a UV-treated room, template was added in a PCR/UV workstation in a separate
room, and single tubes (not strips) were used. For the initial PCR reaction 3-6 μl of the
extracted DNA or freeze/thawed solution was added as template and 1 μl PCR product was
transferred to the subsequent PCR reaction. Five μl from the second PCR reaction was
examined on a 1% agarose gel and for samples that produced a ~359 bp product, the
remaining 20 μl was gel purified (E.Z.N.A Gel extraction kit, VWR). The negative PCR
control and the negative extraction control were always blank. For the negative control PCR
reaction, although there was no visible product the gel region corresponding to the correct
product size was excised, gel purified and cloned. All purified products were cloned using the
TOPO TA Cloning Kit (Invitrogen). Plasmid DNA was obtained using the R.E.A.L Prep96
Farnelid et al.
5
Plasmid Kit (Qiagen) according to manufacturer’s protocol and sequencing was done
commercially (Macrogen, Korea).
Sequence and phylogenetic analysis. Vector sequences and primers were removed manually
and the sequences were translated and aligned using the Lasergene 7 package (DNASTAR).
The most similar uncultured and cultured relatives as identified from BLASTN comparisons
from the NCBI GenBank database were added to the dataset and a neighbor-joining
phylogenetic tree was constructed in MEGA4 (Tamura et al. 2007). The partial nifH
sequences have been deposited in GenBank under accession numbers GU196835-GU196971.
RESULTS
Microscopy analyses of symbionts
The morphologies (e.g. color, shape and size) of symbionts of heterotrophic dinoflagellates
(~100 cells) were compared to published data on ectosymbionts (Table 1) and endosymbionts
(Table 2). All Ornithocercus spp. cells had orange and elongated cyanobacterial
ectosymbionts located within the cingulum, while some also had large rod-shaped non-
cyanobacterial prokaryotes on their sulcal lists (Fig. 1A, large arrow and arrowhead,
respectively; Table 1). These putative ectosymbiotic heterotrophic bacteria were not observed
on Histioneis or Amphisolenia. Histioneis spp. contained two other types of cyanobacterial
ectosymbionts (Fig. 1B, Table 1). In Amphisolenia spp. only endosymbiotic spheres of 3 - 7
µm were found (Fig. 1C, large arrow; Table 2). The endosymbionts in A. bidentata contained
a single yellow chloroplast and a nucleus demonstrating its eukaryotic origin. The symbionts
of A. thrinax had a more brownish color, but whether these symbionts were of a eukaryotic
origin is unclear (not shown).
Farnelid et al.
6
Table 1. Types and characteristics of ectosymbionts of heterotrophic dinoflagellates.
Group Cell shape Length Width Characteristics / Internal structure of symbionts Heterotrophic References (μm) (μm) dinoflagellates (name type) Indian Ocean Synechococcus carcerarius Ellipsoid, 8-10 3-5 Light pink to purplish-red in color Ornithocercus formosus, Norris 1967 Short rod, O. heteroporus, Cylindrical O. magnificus, O. quadratus, O. splendidus, O. thumii, H. carinata, H. dolon, H. pacifica, H. striata Parahistioneis sp.
Synechocystis consortia Spherical 6-8 Grey-Blue in color H. carinata, Parahistioneis sp. Norris 1967
Cyanobacteria Rod/ 1.5-2.8 1.2-1.5 3-4 concentric thylakoids,carboxysomes central Ornithocercus sp. Histioneis sp. Parahistioneis sp.
Lucas 1991 (Type I)
Ellipsoid
Cyanobacteria Rod/ 1.0-1.7 2 Peripheral/central thylakoids, occasional carboxysomes Ornithocercus sp. Citharistes apsteinii Lucas 1991
Ellipsoid (Type II)
Cyanobacteria Spherical 3.5-4.8 Short, irregular thylakoids, few large carboxysomes, many cyanophycin granules
Histioneis sp. Parahistioneis sp.
Lucas 1991 (Type III)
Cyanobacteria Elongate 8-10 1.7-3.3 2-3 peripheral thylacoids, several transverse thylakoids, cluster of carboxysomes, occasional putative cyanophycin granule
O. magnificus, O. quadratus This study
Cyanobacteria Ellipsoid 1.25 Orange color Histioneis spp. This study Cyanobacteria Spherical 2.5-5 - Pale light greenish color Histioneis spp. This study
a : Mean length
6
Farnelid et al.
7
Table 1. (continue) Group Cell shape Length Width Characteristics / Internal structure of symbionts Heterotrophic References (μm) (μm) dinoflagellates (name type) Pacific Ocean Cyanobacteria Spherical/ 1.6±0.6a 1.3±0.4a Prominent glycogen clusters throughout Ornithocercus sp. Foster et al. 2006a Oblong the cytoplasm, carboxysomes scattered often in clusters (Type 1) Cyanobacteria Ellipsoid 2.4±0.6a 1.9±0.5a Sheath, 4-5 concentric peripheral/central Ornithocercus sp Foster et al. 2006a thylakoids, carboxysomes, and glycogen stores central (Type 2) Cyanobacteria Spherical 3.7±0.7a 2.3±0.8a Sheath, large glycogen inclusions throughout cytoplasm, H. depressa Foster et al. 2006a no distinct thylakoids (Type 4) Cyanobacteria Rod/ 1.4±0.5a 1.0±0.3a Glycogen in smaller packets scattered, H. depressa Foster et al. 2006a Spherical diffuse thylakoids and no carboxysomes apparent (Type 5) Cyanobacteria Rod 2.8±0.2a 1.3±0.2a Sheath, 3-4 peripheral thylakoids, small packets Histioneis sp. Foster et al. 2006a of glycogen or occasional as larger inclusion, (Type 6) no visible carboxysomes Cyanobacteria Ellipsoid 1.7±0.6a 0.9±0.1a 4-6 peripheral thylakoids with small packets of glycogen Histioneis sp. Foster et al. 2006a scattered in between, no visible carboxysomes (Type 7) Prochlorococcus? Spherical/ 0.6±0.2a 0.3±0.1a 2-3 peripheral thylakoids, small scattered glycogen packets, Histioneis sp. Foster et al. 2006a Oblong no visible carboxysomes (Type 8) Atlantic Ocean
Cyanobacteria Spherical/ 3.5±0.7a 2.8±0.3a Thylakoids throughout cytoplasm, carboxysomes scatted in O. magnificus Foster et al. 2006a (Type 3),
Rod clusters, glycogen in larger bands between thylakoids Janson et al. 1995 Cyanobacteria Rod/ 2.5 3-4 concentric thylakoids O. magnificus Janson et al. 1995 (type I of Lucas) Ellipsoid Cyanobacteria Rod/ 1-2 Peripheral/central thylakoids O. magnificus Janson et al. 1995 (type II of Lucas) Ellipsoid Cynabacteria Rod 10 1.5 Peripheral thylakoids O. magnificus Janson et al. 1995 (type IV of Lucas)
Heterotrophic bacteria Coccoid 0.4±0.1a -0.5±0.2a
0.4±0.1a - 0.3±0.1a Glycogen scattered throughout the cytoplasm O. magnificus Foster et al. 2006a
(Type b1)
Heterotrophic bacteria Coccoid 1.0±0.4
a - 0.3±0.1a
0.4±0.1a 0.3±0.3a Glycogen scattered throughout the cytoplasm O. magnificus Foster et al. 2006a
(Type b2) a : Mean length
7
Farnelid et al.
8
Table 2. Types and characteristics of endosymbionts of heterotrophic dinoflagellates in the Indian Ocean.
Group Cell shape Length Width Characteristics / Internal structure of symbionts Heterotrophic References (μm) (μm) dinoflagellates (name type) S. carcerarius Ellipsoid, 8-10 3-5 Light pink to purplish-red in color A. globifera Norris 1967 Short rod, Cylindrical Cyanobacteria Rod 10 1.5 Peripheral thylakoids, carboxysomes in rosettes. Amphisolenia sp. Lucas 1991 Oblique division (Type IV) Eukaryotic Spherical - - Golden cells, possibly Chrysophyceae or A. thrinax, Norris 1967 Dinophyceae A. palmata Eukaryotic Spherical 2-3 - 1-2 plastids, a nucleus, mitochondrion A. bidentata, Lucas 1991 A. thrinax Eukaryotic Spherical 3-5 - Single yellow chloroplast and a nucleus A. bidentata This study - Spherical 4-7 - Brownish color A. thrinax This study Heterotrophic bacteria Coccoid, - 0.5 A central core of DNA fibrils, numerous ribosomes A. bidentata, Lucas 1991 Short rod A. thrinax
8
Farnelid et al.
9
Fig. 1. Light microscopy of live cells. (A) Ornithocercus thumii; cyanobacterial ectosymbionts (large arrow).
Notice large bacteria on the sulcal list (arrowhead). The small arrows indicate LCL (lower cingular list),
UCL (upper cingular list), LSL (left sulcal list). (B) Histioneis biremis; two different types of cyanobacterial
ectosymbionts are present (large and small arrows, respectively). (C) Amphisolenia bidentata with numerous
eukaryotic endosymbionts (large arrow). Inset: Details of the endosymbionts. Chloroplast (small arrow);
nucleus (arrowhead).
Farnelid et al.
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TEM revealed that the ectosymbionts of O. magnificus and O. quadratus were cyanobacteria
and heterotrophic bacteria (Fig. 2A, large and small arrows respectively). In both species,
cyanobacterial ectosymbionts were present in the cingulum (Fig. 2A); though a substantial number
was lost during the TEM fixation process. These all appeared to be of the same type, i.e. containing
2 - 3 peripheral thylakoid bands in addition to several bands traversing the cell (Fig. 2E, large
arrow). Clusters of polyhedral granules, carboxysomes (Lucas 1991), were present in all the
cyanobacterial ectosymbionts examined (Fig. 2B, D). In some, electron translucent granules were
present (Fig. 2D), similar to putative cyanophycin granules (Lucas 1991). A typical eubacterial
Gram-negative wall, consisting of a thin wall in-between two membranes, surrounded the
cyanobiont cells (Fig. 2C, arrows). In some Ornithocercus cells, the cingulum also contained small
rod-shaped, 1.5 x 0.2 µm, heterotrophic bacteria (Fig. 2A, F, G). Unfortunately, the large rod-
shaped heterotrophic bacteria seen on the sulcal list by light microscopy (Fig. 1A, arrowhead) were
lost during the TEM preparation procedure.
Farnelid et al.
11
Fig. 2. TEM of Ornithocercus magnificus. (A). Longitudinal section of whole cell of O. magnificus. Four cyanobacterial ectosymbionts and small bacteria are present in the cingulum (large and small arrows, respectively). Numerous rhabdosomes are present within the cell (arrowhead). (B) Longitudinal section of a cyanobiont. Peripheral- (small arrow) and central thylakoid membranes (large arrow); putative carboxysomes (c). (C) The triple-layered cyanobiont wall (small arrows). (D) The putative carboxysomes. (E) Traversing thylakoids (large arrow) and putative cyanophycin granule (arrowhead) of a cyanobiont. (F,G) Details of the bacterial ectosymbionts, in longitudinal (large arrow) and transverse view (arrowhead).
NifH sequence composition and phylogeny
NifH amplicons were obtained from 23 of the 37 analyzed symbiont-bearing heterotrophic
dinoflagellates. The 137 nifH sequences obtained were related to nifH Cluster I (Cyanobacteria and
Alpha-, Beta- and Gamma-proteobacteria), Cluster II (alternative nitrogenases; anfH) and Cluster
III (anaerobic bacteria) as defined by (Chien & Zinder 1996). Sixty-eight% of the sequences
Farnelid et al.
12
(originating from 17 samples) were most similar to proteobacterial nifH sequences (Fig. 3). All ten
sequences from the negative control formed a cluster within Betaproteobacteria (>98% within
cluster sequence identity; Fig. 3) related to a previously reported PCR reagent contaminant
sequence. Four sample sequences (A. bidentata, samples P60 and P62; Table 3) were affiliated with
this cluster, but were not identical to the negative control sequences. Other sequences clustering
with Betaproteobacteria were most similar to Ideonella dechloratans (18 sequences from five
samples; 91-92% sequence identity) and Burkholderia vietnamiensis (12 sequences from five
samples; 97-98% sequence identity) but were clearly distinguished from the negative control
sequences (Fig. 3).
Two of the dinoflagellates contained nifH sequences clustering with cyanobacteria (Fig. 3).
Sample P7 contained sequences of 97 % nucleotide similarity to Nostoc punctiforme while the
sequences from P1 were only distantly related to known phylotypes (<78 % sequence identity).
Three samples contained nifH sequences within nifH Cluster III and two samples contained
sequences related to anfH genes, encoding the iron-only nitrogenase, within nifH Cluster II (Table
3). Twelve sequences, originating from five samples, formed a well supported cluster (bootstrap
99%, Fig. 3), which may represent a novel nifH cluster. These sequences clustered with
Caldicellulosiruptor saccharolyticus (87-98% sequence identity) and with environmental nifH
sequences (EU978414 and EU693383). Five sequences formed a separate cluster only distantly
related to known nifH phylotypes (<69% sequence identity; Fig. 3).
To link sequence composition to dinoflagellate hosts we examined whether a sample
contained single or several nifH sequence types and whether specific nifH sequences were
associated with specific host species. Eight host cells, among which all examined host species were
represented, contained only one nifH sequence type (>99% sequence identity) while nine host cells
contained two or three nifH sequence types each (Table 3). In addition, similar nifH sequences were
found in several hosts and different host species (Fig. 3). For example, 13 nifH
gammaproteobacterial sequences from four samples of different species formed a distinct cluster
with an uncultured nifH phylotype from the Pacific Ocean (DQ481270, 98-99 % sequence identity)
and 13 sequences originating from three samples of different species clustered with Klebsiella
pneumoniae (98-99% sequence identity).
Farnelid et al.
13
Fig. 3. Neighbor-joining phylogenetic tree of nifH-deduced amino acid sequences from symbiont bearing
heterotrophic dinoflagellates. Bootstrap values >50% (1000 replications) are shown. Scale indicates the
number of amino acid substitutions per site. Multiple sequences clustering together are collapsed into
triangles. Sample number and the individual host with the symbionts are indicated in bold with the number
of sequences in brackets
Farnelid et al.
14
Table 3. Phylogenetic affiliations of the nitrogenase gene (nifH) sequences obtained from various
dinoflagellate species. The % sequence identity among the sequences clustering together is indicated in
brackets after the number of sequences per cluster. Below the nifH Cluster (I – III) is indicated with the
phylogenetic affiliation in brackets as designated in Fig. 3. Species Sample
IDAccession numbers Total number of
sequences per sample
P26 GU196890 - GU196894 5 (99.1) 5I (β)
P29 GU196895 - GU196899 4 (99.1) 1 5I (α) ?
P45 GU196923 - GU196930 2 (99.7) 2 (98.2) 1 1 1 1 8? I (γ) I (γ) I (γ) I (β) III
P50 GU196931 - GU196938 5 (99.7) 2 (96.3) 1 8?? I (α) ?
P60 GU196947 - GU196949 3 (99.4) 3I (β)*
P62 GU196950 - GU196953 2 (92.7) 1 1 4I (γ) I (β) I (β)*
P63 GU196954 - GU196960 7 (99.1) 7I (β)
P39 GU196918 - GU196922 3 (98.1) 2 (100) 5II I (β)
P85 GU196966 - GU196971 6 (99.4) 6I (γ)
P01 GU196845 - GU196850 6 (97.8) 6I (**)
P02 GU196851 - GU196855 5 (98.8) 5I (γ)
P53 GU196939 - GU196946 5 (99.4) 2 (99.4) 1 8I (γ) I (β) ?
P06 GU196856 - GU196862 5 (99.4) 2 (98.5) 7I (γ) I (β)
P13 GU196874 - GU196879 6 (96.9) 6I (β)
P14 GU196880 - GU196885 6 (98.8) 6I (γ)
P07 GU196863 - GU196869 3 (99.4) 3 (99.1) 1 7I (**) I (γ) I (γ)
P08 GU196870 - GU196873 4 (99.1) 4I (γ)
P33 GU196916 - GU196917 2 (99.7) 2I (β)
P21 GU196886 - GU196889 4 (98.1) 4II
P31 GU196900 - GU196906 7 (99.4) 7?
P32 GU196907 - GU196915 4 (99.1) 2 (98.8) 1 1 1 9III III III I (β) I (γ)
P66 GU196961 - GU196963 3 (95.9) 3III
P67 GU196964 - GU196965 2 (99.4) 2I (β)
Negative control NTC GU196835 - GU196844 10 (98.5) 10I (β)*
Total 137
α -Alphaproteobacteriaβ -Betaproteobacteriaγ -Gammaproteobacteria* putative PCR reagent contaminant** CyanobacteriaI -nifH Cluster III -nifH Cluster IIIII -nifH Cluster III? Unknown cluster related to Caldicellulosiruptor saccharolyticus?? Unknown cluster distantly related to known nifH phylotypes
O. quadratus
O. steinii
O. thumii
Number of sequences per phylogenetic group
Amphisolenia sp.
A. bidentata
O. heteroporus
O. magnificus
Farnelid et al.
15
DISCUSSION
The role of heterotrophic dinoflagellate symbionts has been a mystery for many years. Due to the
apparent restriction of these consortia to marine waters deplete of inorganic reduced N it has been
suggested that the cyanobacterial symbionts provide their hosts with N through N2 fixation (Gordon
et al. 1994; Jyothibabu et al. 2006; Tarangkoon et al. 2010). In this first report of nifH genes from
dinoflagellate-cyanobacteria consortia, we show that 23 of the 37 investigated dinoflagellate cells
carried putative diazotrophs, and that 21 of these carried nifH genes exclusively related to
heterotrophic bacteria. Hence, our analysis suggests that heterotrophic diazotrophs rather than
cyanobacteria supply the dinoflagellates with reduced N.
Identification of symbionts through microscopy
The identification of symbionts of heterotrophic dinoflagellates has so far primarily been based on
size, shape, pigmentation, and in some cases, ultrastructure. Heterotrophic bacterial ectosymbionts
and/or cyanobacterial ectosymbionts of heterotrophic dinoflagellates have been described from the
Indian, Pacific and Atlantic Oceans (Table 1). The types of ectosymbionts that we observed are
similar to those previously described from the Indian Ocean (Table 1). For instance, the fairly large
ectosymbionts (8 - 10 x 3 - 5 µm) in Ornithocercus magnificus and O. quadratus were similar in
pigmentation, size and shape to Synechococcus carcerarius (Norris 1967, Table 1). This was
supported by a molecular study targeting cyanobacterial 16S rRNA gene sequences from symbionts
of eukaryotic hosts where the majority of the cyanobacterial sequences were closely related to
Synechococcus (>96% similarity; Foster et al. 2006b). Further, the ectosymbionts of Histioneis
carinata and H. biremis both contained at least two types of reddish cyanobacterial ectosymbionts,
a large one (2.5 - 5 µm) and a smaller one (1.25 µm; Fig. 1B), similar to types III and I, respectively
(Table 2 in Lucas 1991).
In accordance with previous observations from the Indian Ocean we found cyanobacterial
and eukaryotic endosymbionts in Amphisolenia spp. (Table 2). Photosynthetic endosymbionts were
observed in both A. bidentata (Fig. 1C) and A. thrinax. So far only one type of prokaryotic
endosymbiont, S. carcerarius, has been reported; originating from A. globifera (Hallegraeff &
Jeffrey 1984; Lucas 1991; Norris 1967), while eukaryotic endosymbionts have been reported from
different species of Amphisolenia (Lucas 1991; Norris 1967). We observed an additional type of
eukaryotic symbiont in A. bidentata. Interestingly, Foster et al. (2006b) also recovered 16S rRNA
Farnelid et al.
16
genes <92% identical to eukaryotic plastids from an A. bidentata host, which could represent the
eukaryotic symbionts as reported by Lucas (1991) and/or in this study (Table 2).
Heterotrophic bacteria have previously been reported as both ecto- and endosymbionts of
heterotrophic dinoflagellates (Tables 1 and 2). For instance, Foster et al. (2006a) detected two
morphotypes of heterotrophic bacteria associated with Ornithocercus magnificus sp. Likewise, we
observed heterotrophic bacterial ectosymbionts of Ornithocercus spp. (Fig. 1A) and both
cyanobacterial and heterotrophic bacterial ectosymbionts for O. magnificus and O. quadratus (Fig.
2A). In O. magnificus and O. steinii, Janson et al. (1995) observed heterotrophic bacteria between
the upper and the lower girdle list of the cingular groove in all samples examined by TEM. Also,
groups of heterotrophic bacterial endosymbionts have been observed in the cytoplasm of A. thrinrax
and A. bidentata (Lucas 1991). Interestingly, although using primers targeting cyanobacteria, 26%
of the 16S rRNA sequences recovered from eukaryotic marine hosts by Foster et al. (2006b)
originated from heterotrophic bacteria; however, none of these could be directly linked to
diazotrophic species (based on BLASTN search results on sequences provided by R. A. Foster).
Thus, the occurrence of heterotrophic ecto- and endosymbionts of heterotrophic dinoflagellates is
not unusual but to our knowledge there is no previous documentation of a N2-fixing potential in
these symbionts.
Identities of nifH genes obtained from symbiont-bearing dinoflagellates
Although cyanobacterial symbionts were visible in all examined dinoflagellates only two of the 23
cells, which yielded nifH sequences, had sequences related to cyanobacteria. Intriguingly, sample
P7 (O. steinii) contained nifH sequences 97% similar to the filamentous heterocystous
cyanobacterium Nostoc punctiforme, which is known from freshwater and for its endosymbiotic
associations with plants (Meeks et al. 2002). Similarly, 16S rRNA gene sequences 92% identical to
Nostoc spp. were found in an A. bidentata host (Foster et al. 2006b). Taken together, these results
may suggest that symbiosis facilitates the survival of Nostoc species in the saline marine
environment. NifH sequences related to cyanobacteria were also obtained from sample P1 (O.
magnificus) but these were only distantly related to known phylotypes (Fig. 3). Since very few nifH
sequences related to cyanobacteria were found we find it unlikely that the role of the cyanobacteria
in the symbiosis should be to supply the host with reduced N. Importantly, in a parallel study using
the same primer sets, we detected representatives from the major groups of unicellular
cyanobacteria (e.g., Crocosphaera watsonii, Cyanothece and Group A; Bergman et al. 1997; Stal &
Farnelid et al.
17
Zehr 2008). Thus, the lack of these known cyanobacteria in the present data set is not due to a
primer mis-match.
Twenty-one samples contained sequences clustering in nifH Cluster I, with Alpha-, Beta-
and Gamma-proteobacteria (Fig. 3, Table 3). NifH gene contamination of PCR reagents, particularly
with Alpha- and Beta-proteobacterial sequences, may occur in the nested PCR (Goto et al. 2005;
Zehr et al. 2003). However, the ten nifH sequences we obtained from non-visible negative control
samples clustered with only four sample sequences (Fig. 3, Table 3) and were not identical to any
sample sequences. Hence, reagent contamination appeared negligible in our study. However,
hypothetical sources of error such as amplification of nifH genes derived from bacteria ingested by
the host or from free-living bacteria which may have been passed through the three washing steps
with 0.2 µm filtered seawater cannot be ruled out. In addition, as the detection limit of the nested
nifH assay is unknown, samples which did not yield a nifH product could theoretically have
contained putative diazotrophs.
Diverse Proteobacteria within nifH Cluster I are commonly detected in marine waters (e.g.,
Church et al. 2005; Hewson et al. 2007; Langlois et al. 2005; Moisander et al. 2008; Zehr et al.
1998). Associated with dinoflagellates, we found eight diverse clusters of nifH sequences related to
Gammaproteobacteria while only two sequences were related to Alphaproteobacteria (Fig. 3).
Interestingly, 30 sequences (22% of all sequences) were affiliated with two betaproteobacterial
clusters, distinct from the negative control sequences (Fig. 3). Similarly, bacteria associated with
the photosynthetic dinoflagellate Gyrodinium instriatum were dominated by Betaproteobacteria
(Alverca et al. 2002). Hence, although rare in marine ecosystems (Barberán & Casamayor 2010),
Betaproteobacteria appear common as symbionts of dinoflagellates.
Sequences clustering in nifH Cluster II were obtained from two samples (O. heteroporus
P39 and O. thumii P21, Table 3). Mo- independent nitrogenases are present in a diverse group of
diazotrophs and second alternative nitrogenases are expressed under Mo- and V- deficient
conditions (Betancourt et al. 2008). Bacteria containing alternative nitrogenase genes have been
isolated from diverse marine environments (Loveless et al. 1999) but interestingly anfH related
genes seem to be absent in sub-tropical and tropical open waters (e.g., Church et al. 2005; Hewson
et al. 2007; Langlois et al. 2005; Moisander et al. 2008; Zehr et al. 1998). Thus, the recovery of
anfH related genes suggests that symbionts of dinoflagellates may be an environmental niche in
open water where second alternative nifH genes can be used.
Farnelid et al.
18
Sequences from Cluster III, which contains nifH genes from anaerobic bacteria, have been
detected (Church et al. 2005) but appear uncommon in the open ocean (Langlois et al. 2005;
Langlois et al. 2008). The presence of Cluster III sequences in three dinoflagellates therefore
suggests that the cyanobacteria-dinoflagellate consortia provide low oxygen (O2) habitats required
for N2 fixation (Paerl & Prufert 1987). Similarly, Cluster III sequences from strict anaerobes and
nitrogenase activity have been detected in association with zooplankton (Braun et al. 1999).
However, since our Cluster III sequences were only distantly related to cultivated anaerobic bacteria
(76-87% sequence identity) the phenotypes they represent are rather uncertain. Survival of strict
anaerobes associated with dinoflagellates would presumably require vertical inheritance of these
symbionts as the dinoflagellate host divides. However, given the observed non-host specificity for
the symbionts (see below), it may be more likely that the obtained Cluster III sequences derive from
facultatively anaerobic bacteria.
Twelve sequences originating from five samples formed a discrete nifH cluster separate
from the known nifH clusters I-IV (Chien & Zinder 1996; Fig. 3). These sequences were 87-98%
similar to the nifH gene of C. saccharolyticus (van de Werken et al. 2008), which can grow in the
absence of reduced N (van Niel et al. 2002). It is surprising to find sequences closely related to an
anaerobic extreme thermophile in the pelagic zone; however, the cluster also contains nifH
sequences from a marine bloom and from symbionts of corals. Hence, surface-associated growth in
the marine environment, like in association with dinoflagellates, may be characteristic for these
bacteria.
Eleven clone libraries yielded two or three different nifH sequence types per dinoflagellate.
This suggests the presence of mixed assemblages of diazotrophic symbionts in host cells (Table 3),
consistent with previous microscopy observations of mixed populations of cyanobionts and/or
bacterial cell types in one host cell (Foster et al. 2006a). In addition, observations of several
specimens of the same dinoflagellate species with diverging nifH sequences and different species of
dinoflagellates hosting identical nifH sequences suggested that the nifH phylotypes were not host
specific. Similar patterns of non-host specific 16S rRNA gene phylotypes were also observed for
cyanobacterial symbionts in ciliates, dinoflagellates, and radiolarians (Foster et al. 2006b). In
contrast, in the Richelia intracellularis-diatom symbiosis a divergence of hetR and nifH sequences
of symbionts from different host species was interpreted as an indication of host specificity (Janson
et al. 1999, Foster & Zehr 2006). Thus, it appears that at any one time dinoflagellate hosts may
contain multiple symbionts but the low degree of specificity also indicates that their dependence on
specialized symbionts is not fundamental.
Farnelid et al.
19
Putative ecological roles of the consortia
In dinoflagellate-cyanobacteria consortia the host’s requirement for fixed carbon as well as N is
presumably the driving force for the relationship. Our results show that heterotrophic bacterial
symbionts rather than cyanobionts have the genetic potential for fixing N2. Consequently, we
speculate that the widespread, and somewhat counter intuitive distribution of these large (50 - 1000
µm) species of heterotrophic dinoflagellates in the oligotrophic subtropical and tropical oceans is
partly made possible by symbiont-mediated photosynthesis (cyanobacteria) and N2 fixation
(heterotrophic bacteria).
Acknowledgements
Danish Galathea expedition and the Captain of HMDS ‘Vædderen’, Carsten Smidt, and his crew are
thanked for excellent assistance in connection with sampling. We thank R.A. Foster for generously
providing unpublished sequence data. The project was supported by grants from Knud Højgaards
Fond, Danish Natural Sciences Research Council (272-05-0333 and 272-06-0485 to P.J.H. and 277-
05-0421 to G.H.) and Dansk Expeditions fond. The work of H.F. was supported by the Swedish
Research Council FORMAS (217-2006-342 to L.R.). The present work was carried out as part of
the Galathea 3 expedition under the auspices of the Danish Expedition Foundation. This is Galathea
3 contribution no. xx.
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Appendix-I Contributions in paper
Woraporn Tarangkoon (WT), Per Juel Hansen (PJH), Gert Hansen (GH), Morten Moldrup (MM), Øjvind Moestrup (ØM), Lydia Garcia-Cuetos (LG), Hanna Farnelid (HF), Lasse Riemann (LR)
Paper I II III Appendix-IV Original idea PJH, WT PJH, WT PJH,WT, MM PJ, WT Study design and method
PJH, WT PJH, WT PJH,WT, MM PJ, WT, HF, LR
TEM work GH, WT - ØM GH,WT Molecular Work
- - LG HF, LR
Data gathering WT WT PJ, WT, MM, ØM, LG
HF
Writing WT, PJ, GH WT,PJ WT, PJ, MM, ØM, LG
HF, LR, PJ, WT, GH