MIqPCR

A Untergasser

Scope of the guidelines

Aims to describe quantitative PCR.

Technical guideline to create a meaningful

RDML file correctly (overcome limits of XML).

Extended/replaced by MIQE in future.

Level of implementation

MIqPCR was published with RDML v1.0.

MIQE is in the focus of the journals.

qPCR-Ninja is developed to support RDML and

MIQE.

Status, contributors, funding

MIqPCR is dead.

MIQE is under active development

MIQE

Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT

Scope of the guidelines

MIQE - minimum information for publication of

quantitative real-time PCR experiments

entire qPCR workflow

experimental design

sample information

sample processing

target information

oligos and probes

qPCR protocol

qPCR validation

data analysis

Level of implementation

creation of public awareness presentations on numerous scientific meetings

company tours

part of qPCR courses

letter to editors

follow up papers

standard data format RDML (listed as MIqPCR @ MIBBI)

cited in 185 papers since 2009

guidelines adopted by journals Clinical Chemistry

Nucleic Acids Research

BMC-Series journals

RDML

1.0 since 2008

1.1 to be released soon

2.0 planned

Taken up by different companies

Life Technologies

Bio-Rad

Biogazelle

Status & funding

Continued effort to create public awareness

Active discussion with industry on what is acceptable

under the MIQE label

Planning for MIQE 2.0

Strengthen link with RDML

Enhance development of website

No dedicated MIQE funding