MICROSCOPEMICROSCOPE

Microscope is a tool that is used to Microscope is a tool that is used to observed microscopic objects that cannot observed microscopic objects that cannot be seen with the naked eyes.be seen with the naked eyes.

A light microscope works by means of A light microscope works by means of centralizing light caught by our eyes to centralizing light caught by our eyes to form a magnified image of an object.form a magnified image of an object.

ObjectivesObjectives

To know parts and their functions of a To know parts and their functions of a microscopemicroscope

To use a microscope correctlyTo use a microscope correctly

Stereo microscope Light microscope

A stereo microscope is often used for lower-power

magnification on large subjects

Electron Electron MicroscopeMicroscope

Electron microscopes, which use beams of electrons instead of light, are designed for very high magnification usage.

Electrons, which have a much smaller wavelength than visible light, allow a much higher resolution.

MirrorMirror

Objective lensObjective lens

Hole Hole

DiaphragmDiaphragm

EyepieceEyepiece

Body tubeBody tube

Revolver Revolver

CoaCoarrse se AdjustmentAdjustment

Stage Stage

Stage ClipsStage Clips

BaseBase

ArmArm

Fine Fine AdjustmentAdjustment

Microscope PartsMicroscope Parts

Microscope Parts   

Body tube

Base

Stage Clips

Revolver

Arm

Parts of Microscope and their functionParts of Microscope and their function

Eyepiece (Lensa Okuler)Eyepiece (Lensa Okuler): to magnify image you : to magnify image you look throughlook through

Coarse Coarse adjusmentadjusment (Pemutar Kasar) (Pemutar Kasar):: focuses the focuses the image under low powerimage under low power

Body tube (Tabung)Body tube (Tabung): connect the eyepiece to the : connect the eyepiece to the revolving nose piecerevolving nose piece

Fine Fine adjustmentadjustment (Pemutar Halus) (Pemutar Halus):: sharpens the sharpens the image under high and low magnificationimage under high and low magnification

Revolver (Pemutar Lensa)Revolver (Pemutar Lensa): holds and turns the : holds and turns the objectives into viewing positionobjectives into viewing position

Low-power objective Lens (Lensa Objektif Low-power objective Lens (Lensa Objektif Pembesaran Lemah)Pembesaran Lemah):: contains the lens with contains the lens with lower power magnificationlower power magnification

High-power objective Lens (Lensa Objektif High-power objective Lens (Lensa Objektif Pembesaran Kuat)Pembesaran Kuat):: contains the lens with high contains the lens with high (the most) magnification(the most) magnification

Arm (Lengan Mikroskop)Arm (Lengan Mikroskop): support the body tube.: support the body tube.

Stage (Meja Benda)Stage (Meja Benda): platform used to support the : platform used to support the microscope slidemicroscope slide

Stage clips (Penjepit)Stage clips (Penjepit): hold the microscope slide in place: hold the microscope slide in place

Diaphragm (Diafragma)Diaphragm (Diafragma): regulates the amount of light : regulates the amount of light entering the body tubeentering the body tube

Mirror (Cermin)Mirror (Cermin): allows light to go upward through the : allows light to go upward through the diaphragm, the specimen, and the lensesdiaphragm, the specimen, and the lenses

Base (Kaki Mikroskop)Base (Kaki Mikroskop): provides support for the : provides support for the microscopemicroscope

How to figure out the How to figure out the Magnification?Magnification?

Low Power 10 X 10 X 100 XLow Power 10 X 10 X 100 X

Eyepiece Objective Lens Magnification

High Power 10 X 45 X 450 X

Working with your microscope Working with your microscope !!

1.1. Place the microscope on flat surface. The Place the microscope on flat surface. The arm should be toward you.arm should be toward you.

2.2. Look through the eyepiece. Open the Look through the eyepiece. Open the diaphragm.diaphragm.

3.3. Adjust the mirror until you can see the most Adjust the mirror until you can see the most light coming through the tube. light coming through the tube.

Seven easy steps Seven easy steps for beginners !for beginners !

4.4. Place the slide so that the material to be Place the slide so that the material to be observed is in the middle of the hole on the observed is in the middle of the hole on the stage. stage.

5.5. Turn the nosepiece to the low- or medium-power Turn the nosepiece to the low- or medium-power objective- objective- never start to the high-powernever start to the high-power. .

6.6. Turn the coarse Turn the coarse adjustmentadjustment until the objective until the objective goes as far as it can without touching the slide.goes as far as it can without touching the slide.

7.7. Finally, look through the eyepiece and sharpens Finally, look through the eyepiece and sharpens the image by turning the fine the image by turning the fine adjustmentadjustment. .

Attentions !!Attentions !!

1.1. Always carry the microscope by holding the Always carry the microscope by holding the arm with one hand and supporting the base arm with one hand and supporting the base with the other hand.with the other hand.

2. Do not touch the lenses with your finger.2. Do not touch the lenses with your finger.

3. Always focus first with lower-power objective.3. Always focus first with lower-power objective.

4. Do not use coarse 4. Do not use coarse adjustmentadjustment when the high when the high power objective is in place.power objective is in place.

Once you have examined Once you have examined the specimen under low the specimen under low

power, try this:power, try this: fiddle with the mirror. Sometimes you can change fiddle with the mirror. Sometimes you can change

the light and see the specimen in a different way. the light and see the specimen in a different way.

as you look through the eyepiece, try moving the as you look through the eyepiece, try moving the slide to see different parts of the specimen. This slide to see different parts of the specimen. This takes practice. takes practice.

try a high-power objective, but expect the light to be try a high-power objective, but expect the light to be dimmer. You will probably have to change the focus dimmer. You will probably have to change the focus a bit. a bit.

Position of Object Glass and Cover Glass