Microbiology Laboratory Microbiology Laboratory Safety and RulesSafety and Rules

Biology IIBiology II

Mrs. HienemanMrs. Hieneman

#1#1

• Do not bring food, drink, or Do not bring food, drink, or tobacco into the lab.tobacco into the lab.

• Do not invite friends into the Do not invite friends into the lab.lab.

• Keep pencils, fingers, etc. out of Keep pencils, fingers, etc. out of your mouth, ears, eyes, nose, your mouth, ears, eyes, nose, etc.etc.

#2#2

• Wear protective clothing at all Wear protective clothing at all times.times.

• Shoes are always requiredShoes are always required• Open-toed sandals are not Open-toed sandals are not

allowed.allowed.

#3#3

• Do not take any cultures out of Do not take any cultures out of the lab for any reason.the lab for any reason.

#4#4

• Wipe the bench tops down with Wipe the bench tops down with disinfectant both before you disinfectant both before you begin your work and after you begin your work and after you have completed your work.have completed your work.

#5#5

• Keep nonessential books and Keep nonessential books and clothing far away from your clothing far away from your work area.work area.

#6#6

• Wash your hands with soap and Wash your hands with soap and water for 15 seconds before and water for 15 seconds before and after you work in the lab, or if you after you work in the lab, or if you leave the lab for any reason.leave the lab for any reason.

#7#7

• Do not mouth pipet ever!!!!!!!!!!Do not mouth pipet ever!!!!!!!!!!

#8#8

• Be careful near bunsen burners.Be careful near bunsen burners.• Tie long hair back away from Tie long hair back away from

the face.the face.• Turn off burners when not in Turn off burners when not in

use.use.

#9#9

• Beware of the chemicals and Beware of the chemicals and reagents used in biochemical reagents used in biochemical tests.tests.

#10#10

• Report any accidents (spills, broken Report any accidents (spills, broken glass, cuts, or injuries) to your glass, cuts, or injuries) to your instructor immediately.instructor immediately.

#11#11

• Dispose of contaminated or used Dispose of contaminated or used materials quickly and in the materials quickly and in the manner set forth by your manner set forth by your instructor.instructor.

#12#12

• Label all of your tubes and plates Label all of your tubes and plates exactly as directed by your exactly as directed by your instructor.instructor.

• Place all cultures for incubation Place all cultures for incubation exactly where instructed or you exactly where instructed or you risk their loss.risk their loss.

• Any cultures not handled Any cultures not handled accordingly will be discarded!!!accordingly will be discarded!!!

#13#13

• Keep microscopes clean and the Keep microscopes clean and the objectives free of oil.objectives free of oil.