microbiology analytical service guide - a&b labs

A&B ENVIRONMENTAL SERVICES, INC.

Microbiology TestingLaboratory Service Details

2008

NELAP CERTIFIED & AIHA ACCREDITED LAB

10100 East Freeway Houston, TX 77029Toll Free: 877.478.6060

(P) 713.453.6060 (F) 713.453.6091ablabs.com

MICROBIOLOGY TESTING SERVICE GUIDETABLE OF CONTENTS

Microbiology Terms and Conditions…………..……………………...…...……..BIO 3

A&B Labs Map of Certification States for Environmental Testing…………….BIO 8

Microbiology Testing Pricing

Alphabetical Order of Microbiology Testing……….….………..………..BIO 9

Terms and Abbreviations……………………………………………………....….BIO 15

Phone: 713-453-6060 BIO 2 www.ablabs.com

– BIO 3 –A&B LABS MICROBIOLOGY TERMS AND CONDITIONS 01.10.2008

MICROBIOLOGY TERMS & CONDITIONS

A&B LABS is an independent minority owned analytical laboratory established in1988. We offer an extensive array of analytical capabilities in environmental, industrialhygiene, microbiology and food safety areas. A&B Laboratories has an unmatchedcommitment to quality data and personalized service. We utilize EPA, NIOSH, OSHA,FDA/BAM and AOAC methodology.

At A&B Laboratories, our mission is to deliver quality analytical services to our clientswhile following ethical practices and providing stellar customer service with continualimprovement.

ANALYTICAL REPORTS AND DATA DELIVERABLE PACKAGES

A&B Labs provides a Level II QA Report Deliverable Package as our standard reportingpackage. This report includes a case narrative, spiked sample and spiked duplicateresults, surrogate recoveries, laboratory control, blank results, sample conditionchecklist and sample chain of custody. PDF copies of reports are available and A&BLabs reserves the right to send PDF summary report pages whenever possible. Thestandard analysis turnaround time for Level II Reports is seven (7) Business days. Hardcopy reports will be mailed within 48 hours after the reports are sent via email to theclient. Original analytical reports, invoices and original chain of custodies will be sent tothe client as well.

ELECTRONIC DELIVERABLE PACKAGES (EDD’s)

A&B Labs can provide client specific EDD’s to meet specifications. Surcharges for suchpackages may apply. Contact Sales and/or Project Management for more information.

On-line capabilities include:ƒ Viewing of results via the webƒ Access data 24/7ƒ Access complete PDF reportsƒ Download results in ExcelFormat

ƒ View summary of completed testsƒ Track the status of samplesƒ Sample receipt notification

TURNAROUND TIME AND RUSH POLICY

The Standard Turnaround Time for A&B Labs is Seven (7) Business days. A&B Labscounts the turnaround time as follows: Day Zero (0) is when our lab receives the project;Day One (1) is counted as the next business day. Weekend and holidays are notcounted for Turnaround Time Calculations. Our normal working hours are Monday-Friday, 8am-5pm.



To guarantee Rush Turnaround Time for your project, all rush requests must beconfirmed with the lab prior to shipment of samples. A&B Labs cannot and will notguarantee rush request unless lab is notified in advance. The following surcharges willapply for Rush Turn Around Times:

Rush Turn Around Time SurchargeSame Day 200%

1 Day 100%2 Day 75%3 Day 50%5 Day 25%

Some parameters may not be able to be performed within a 24 or 48-hour period. TheLab may limit the number of Rush samples accepted in one day. 1 Day Rush sampleswill be reported by 5:00 PM (Central Time) the day after the samples are received at thelab. 1 Day Rush samples may only be able to be analyzed once within the first 24-HourRush. TAT may not be able to be met if dilutions are required, or if matrix interferencesare present. In this case, estimated values may be given to the client within 24 hoursafter receipt of the sample with the surcharge still applicable.

INVOICING POLICY

A&B Labs will invoice the client with a copy of analytical reports and original chain ofCustody (COC). Invoices will reflect prices listed in the quotation setup by your salesand/or project manager. Any parameters analyzed that are not included on the pricingagreement will be billed at A&B Lab’s 2008 list price less 10%. The client is responsiblefor supplying any required P.O. Numbers, Project Code Numbers, etc. Terms are Net30 days. For International projects payments arrangement must be made in advance.

SAMPLE MEDIA AND SAMPLE PICKUP POLICY

A&B Labs will provide routine sample kits at no additional charge provided thelaboratory is given at least 24 hours notice, in some cases charges may be applied forrush kits. These routine kits include the proper bottles, preservations, labels, chain ofcustody forms and Federal Express return shipment forms with A&B Lab’s ID number.Cassettes and other media may be available for an additional charge. A&B Labs mayalso agree to provide sample pick-up free of charge within a 25-mile radius of ourfacility, please contact the A&B Sales and/or Project Management Department todetermine if this service will be available for your site.

SHIPMENT OF OUTGOING MEDIA FROM A&B LABS TO THE CLIENT

A&B Labs will pay for the outgoing media shipment costs only if the kit order is receivedearly enough (typically 4 to 5 business days) to use the most cost efficient means ofshipping available (Examples: UPS Ground Service, FedEx Ground, FedEx Express3Day etc).


For International shipping, the client is responsible for shipping cost both outbound andinbound. Any other outgoing expedited shipments (FedEx Priority and StandardOvernight, 2 Day, etc.) will be invoiced to the client. Charges for expedited shipmentswill depend on the weight of the shipment and the applicable zone charges.

Notes:

A&B Labs agrees to provide sample bottles for samples to be analyzed by A&B Labsonly. The client or the consultant must supply any sample bottles required for analysisby any other lab, or to be used for field screening by the consultant or any otherpurposes.

Sufficient time should be given to ship kits by routine ground delivery (4 to 5-Daynotice), thus avoiding a priority shipment charge. The client should contact A&B Labswhenever a kit is being returned for Saturday delivery, as special Fed Ex proceduresare required to be completed on the Air bill. A&B Labs will not be held responsible forbreakage of sample bottles during the return shipment to the lab.

SAMPLING PROCEDURES

LIQUIDS/SOLIDS

Please properly pack and ice down return sample kits to keep temperature at the EPArequired 4°C for liquid or solid samples. The holding time liquid or solid samples is six(6) hours and a minimum of 4 ounces is required. Water samples only require 100milliliters of sample. All liquid or solid samples must be packed in a sterile glass orplastic container. A sterile bag is also acceptable.

FOOD

All food should be kept at the original temperature as well as in the original storagecontainer. Food should be sent or brought to the laboratory as soon as possible. If thefood cannot be stored in the original container, then a sterile bag or container isrequired.

RETURN SHIPMENT OF SAMPLE KITS FROM THE FIELD TO A&B LABS

Any shipments incoming from the site to the lab, whether by FedEx, UPS, or anothercarrier, will be invoiced to the client. The client will be responsible for the properlabeling of the kits. Please note that Federal Express requires special procedures forshipments to be delivered to the lab on Saturday.

– BIO 5 –A&B Labs Microbiology Terms and Conditions 01.10.2008


A&B Labs reserves the right to reject samples for any of the following reasons:ƒ Chain of Custody documentation not includedƒ Discrepancies between the Chain of Custody and sample labelsƒ Incomplete sample containers for the analysis requestedƒ Inappropriate preservation for the analysis requestedƒ Sample received exceeding hold time for analysis requestedƒ Inappropriate sample labeling (not using water resistant labels and indelible ink)ƒ Samples with signs of damage or contamination

ƒ Samples containing high levels of potentially dangerous substances or vaporsthat may cause health or safety problems during sample preparation or analysis

REPLACEMENT SAMPLE POLICY

If A&B Labs fails to meet regulatory hold times or has specific errors, which are due tothe sole fault of the laboratory, A&B Labs will either retest the sample or a replacementsample at no additional charge. A&B Labs will not be held responsible for any re-sampling costs as a normal procedure. A&B Labs will also not be liable for any chargesdue to delay by any courier used for shipment of the samples including (weather,equipment failure, acts of God, etc.)

SAMPLE DISPOSAL POLICY

A&B Labs will agree to hold all sample remainders for thirty (30) days after the finalreport is issued. Request to hold samples by A&B Labs beyond this date will be chargedat a rate of $3 per sample per day. A&B Labs reserves the right to charge $3 persample for sample disposal. Disposal of samples is controlled and specified by A&BLabs’ SOP (Standard Operating Procedures).

ƒ Samples determined by analysis or other characteristics to be hazardousmaterials will either be returned to the client or be disposed of as follows:

ƒ The samples shall be composite as waste material. The waste shall be stored indrums, which meet or exceed both RCRA and DOT specifications.

ƒ All drums will prominently display the appropriate DOT and RCRA labelsidentifying the general contents of the drum while on-site. The drumidentifications will meet the requirements of the contracted RCRA waste disposalservice prior to off-site shipments.

ƒ All disposals of both wastes shall be fully compliant with applicable Federal andState regulations. A&B Labs uses EPA and Texas Natural ResourcesConservation Commission approved TSD facility for handling all hazardouswaste.



GENERAL WARRANTY

A&B Labs warrants that all work will be preformed in accordance with sound laboratorypractice and accepted analytical procedures, but make no other warranty, expressed orimplied. A&B Labs is solely responsible for performance of this contract, and noaffiliated company, director, officer, employee, agent or subcontractor of A&BLabs shall have any legal responsibility hereunder, whether in contract or tort, orotherwise. Liability for injury or damage to persons or property arising out of this work(other than for professional errors, omissions, or other professional negligence, whichare covered by the next sentence) shall be limited to the amount covered by the generalcomprehensive insurance carried by A&B Labs. In the event of error, omission, or otherprofessional negligence or any breach of the above warranty, the sole and exclusiveresponsibility of A&B Labs shall be to re-perform the deficient work at its own expense,and A&B Labs shall have no other liability whatsoever. In no event shall A&B Labs beliable, whether in contract or tort, or otherwise for any incidental consequential orspecial damages including but not limited to, damages in any way connected with orinterpretation of information or analysis provided by A&B Labs or with action related tosuch use or interpretation.


A&B Environmental Services, Inc.State Certification 2008

Microbiology

Certified or No Certification Required

Not Certified

Microbiology Testing Service Guide

Analytical TestMatrix Sample Size

ContainerPreservation Holding Time

Leach/Extr/AnalyMethod


Actinomyces sppw 100 ml p 4ºC 8 hours SSSAs 4 oz p 4ºC N/A SSSA

Aerobic Plate Count (APC) / TPC/Standard Plate Countf 4 oz/ orig. cont. as is 8 hours CMMEF 7.63

Aerobic Plate Count (cosmetics/products-bam)Liq/Sol 4 oz p N/A N/A BAM 23H1

Aerobic Plate Count-N Certified APC / HPC / TPCws/ww

4 oz p 4ºC4 oz p 4ºC

6 hours6 hours

SM 9215BSM 9215B

2 dilutions max, $20 for each additional dilution

Aerobic Plate Count-Petrifilmf 4 oz/ orig. cont. as is ASAP AOAC

Aeromonasw/s 4 oz p N/A N/A AOAC/CMMEF

Algal countw/ww 4 oz p 4ºC 6 hours SM

Anaerobic Plate Countw/s 4 oz p 4ºC 6 hours 9215B mod.

Bacterial Count with Gram StainB/Sw/Wi/a 4 oz p 4ºC 6 hours AOAC

Bacterial ID per SpeciesB/Sw/Wi/a 4 oz p 4ºC N/A Various

Camplylobacter-Nf 4 oz/ orig. cont. as is 6 hours CMMEF

Campylobacter-Nw 4 oz p 4ºC 6 hours SM9260G

Clostridium perfringensw/s 4 oz p 4ºC 6 hoursf 4 oz/ orig. cont. as is ASAP AOAC/CMMEF






Denitrifying Bacteriaw 100 ml p 4ºCs 4 oz p N/A

8 hours6 hours

SSSASSSA

Denitrifying Bacteria Identificationw 100 ml p 4ºCs 4 oz p N/A

6 hours6 hours

SSSASSSA

Drinking Water Package (Total Coliform P/A and HPC)w 100 ml p 4ºC 6 hour SM 9223B

E. Colif 4 oz/ orig. cont. as is ASAP CMMEF 35.24

E. Coli H7:0157 including H7-P/Aw 100 ml p 4ºCf 4 oz/ orig. cont. as is

6 hoursASAP

SM 9260FAOAC 996.09

E. Coli-IDEXX-N Certifiedw 100 ml p 4ºC 6 hours SM 9223B

E. Coli-MF-Nw 100 ml p 4ºC 6 hours SM 9222G

E. Coli-N-Petrifilmf 4 oz/ orig. cont. as is ASAP CMMEF/AOAC

E.Coli-Chromogenic P/A Certifiedw 100 ml p 4ºC 24 hour SM 9223B

Enterococcus-Ns/ww 4 oz p 4ºC 6 hours SM 9230C

Fecal Coliforms-MF-N Certifiedw 100 ml p 4ºC 6 hours SM 9222D

Fecal Coliforms-MTF-4 dil-N certifiedw 100 ml p 4ºC 6 hours SM 9221E

Fecal Coliforms-Ns/ww 4 oz p 4ºC 6 hours SM 9222D






Fecal Streptococcus mFw 100 ml p 4ºC 6 hours SM 9215B

Fecal Streptococcus-Ns/ww 4 oz p 4ºC 6 hours SM 9230C

Filth Testvarious 4 oz p 4ºC NA AOAC 945.80

Flavobacterium spp.w/sf

4 oz p N/A4 oz/ orig. cont. as is

ASAPASAP

VariousVarious

Hemolytic Bacterial Countw 100 ml p 4ºCs 4 oz p N/A

6 hours6 hours

AOAC/CMMEFAOAC/CMMEF

Heterotrophic Plate Count (HPC) / APC / TPCws/ww

100 ml p 4ºC4 oz p 4ºC

6 hours6 hours

SM 9215BSM 9215B

2 dilutions max, $20 for each additional dilution

Iron Depositing Bacterias/w 4 oz p 4ºC 6 hours SM 10200

Klebsiella spp.f 4 oz/ orig. cont. as is ASAP AOAC/CMMEF

Lactic acid bacteria (Petrifilm)f 4 oz/ orig. cont. as is ASAP AOAC

Legionella-air culturea air sampler N/A (RT) N/A SM 9260J

Legionella-spp-P/Aw 100 ml p 4ºC 6 hours SM 9260J

Listeria monocytogenesf 4 oz/ orig. cont. as is ASAP AOAC/CMMEF

Listeria spp-P/Af 4 oz/ orig. cont. as is ASAP CMMEF BAM/AOAC






Luminometer Testw/s 4 oz p 4ºC 6 hours Bioluminescence

Microscopic Examvarious 4 oz p 4ºC N/A SM 10200

Mold by Agar Plate ID 3spp.w/s 4 oz p N/A 6 hours Viable

Mold by Culture CountsB/Sw/Wi/A 4 oz p N/A (RT) 6 hours Viable

Mold by Culture ID-3 speciesB/Sw/Wi/A 4 oz p N/A (RT) 6 hours Viable

Mold by Direct ExamB/Sw/Wi/T Air Cassette N/A N/A Microscopy

Pseudomonas Aeroginosaw 100 ml p 4ºC 6 hours SSSA/SMs 4 oz p N/A 6 hours SSSA/SM

Salmonella Composites 4 oz. P N/A N/A AOAC/CMMEFf/w 4 oz p as is/ 4ºC 6 hours AOAC/CMMEF

Salmonella Sero ID Onlys 4 oz. P N/A 6 hours AOAC/CMMEFf/w 4 oz p as is/ 4ºC 6 hours AOAC/CMMEF

Salmonella spp.w 100 ml p N/A 6 hours SM 9260Bf 4 oz/ orig. cont. as is ASAP CMMEF BAM/AOAC

Sewage Scres/sw

en: Moist, dry,4 oz p

soil, waste water: Package N4ºC 6 hours SM various $120Shigella spp

f 4 oz/ orig. cont. as is ASAP CMMEF BAM/AOAC

Shigella spp.w 100 ml p N/A 6 hours SM 9260E/ AOAC






Staphylococcus Aeureus-N Petrifilmf 4 oz/ orig. cont. as is ASAP CMMEF/AOAC

Staphylococcus Aureus Coagulasef/w/s Sterile Bag as is/ 4ºC 8 hours AOAC/CMMEF

Staphylococcus spp.-Nw 100 ml p 4ºC 6 hours SM 9213B

Sulfate Reducing Bacteriaw/s 4 oz p 4ºC 6 hour SM 9240D

Total Coliform VRBAw/f/s 4 oz p 4ºC 6 hours AOAC/CMMEF

Total Coliforms Chromogenic P/A Certifiedw 100 ml p 4ºC 24 hours SM 9223B

Total Coliforms-IDEXX-Nw 100 ml p 4ºC 6 hours SM9223B

Total Coliforms-MF-N certifiedw 100 ml p 4ºC 6 hours SM 9222B

Total Coliforms-Ns/ww 4 oz p 4ºC 6 hours SM 9222D

Total Coliforms-Petrifilmf 4 oz/ orig. cont. as is ASAP CMMEF/AOAC

Total Free Nitrogen Fixers + Bradirhizobiumw 100 ml p 4ºC 6 hours SSSAs 4 oz. P N/A 6 hours SSSA

Total Plate Count (TPC) / APC / HPCw 100 ml p 4ºC 6 hours SM 9215Bs/ww 4 oz p 4ºC 48 hours SM 9215B2 dilutions max, $20 for each additional dilution

Total Plate Count-Nf 4 oz/ orig. cont. as is ASAP CMMEF 7.63

Vibro spp-P/A






liquid/solid 4 oz p N/A N/A Bam 23H2

Yeast and Mold-Nw/s/liq 100 ml p 4ºC 6 hours SM 92610B

Yeast and Mf/solid

ld-N (3-5d)4 oz/ orig. cont. as is ASAP CMMEF/BAM $50Yersinia spp

w/s 4 oz. P N/A 6 hours AOAC/CMMEFf 4 oz/ orig. cont. as is ASAP AOAC/CMMEF

w 100 ml p 4ºC 6 hours SM 9260Hf 4 oz/ orig. cont. as is ASAP CMMEF BAM

Yeast and Mold in Cosmetics

o

Terms and Abbreviations

a airAOAC Association of Official Analytical ChemistsB BulkBAM Bacteriological Analytical ManualC air-o-cell CasetteCMMEF Compendium of Methods for the Microbiological Examination of Foodsd day(s)E.coli Escherichia colif foodG Glassg GramID Identificationml millileterorig. cont Original Containeroz ouncemod ModifiedL LiterM ModifiedN/A Not ApplicableN Numbersp Polyethylene/PlasticP/A Presence or Absencep/g Plastic/GlassRT Room Temperatures SoilSM Standard Methodspp SpeciesSSSA Soil Science Society of AmericaSw Swabsw Solid WasteT TapeVRBA violet red bile agarw Waterww Waste Water


microbiology analytical service guide - a&b labs

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