Garantirana detekcija in kvantifikacija z uporabo laboratorijsko preverjenih testov

Zanesljivi rezultati z integriranimi kontrolami

Pripravljena programska orodja za preprosto interpretacijo rezultatov

Microbial DNA qPCR Assays (330025) 30% OFF

1X 100 µl tube Microbial DNA qPCR Assay, 1X 1.35 ml tube Microbial qPCR Mastermix

Izbor testov: Microbial DNA qPCR Assay/Assay Izbor testovza odpornost na antibiotike: Antibiotic Resistance Gene Resource and Assay List

Microbial DNA qPCR Assay Kit (330033) 30% OFF

1X 20 µl tube of Microbial DNA qPCR Assay, 1X tube Positive PCR Control (20 µl), 1X tube Microbial DNA Positive

Control (50 µl), 1X tube Microbial DNA-Free Water (1.35 ml), 1X tube Microbial qPCR Mastermix (1.35 ml)

Microbial DNA qPCR Arrays (330261)

Pred pripravljeni hidrolizirani testi so naneseni na različnih PCR ploščah 96 ( pakiranje 2/12/24), 384 (pakiranje

1/4/16), 100 mestni okrogli nosilec, primernih za različne qPCR naprave. PCR miksi lahko vsebujejo ROX, Fluorescin ali

so brez dodatka. Arreji vsebujejo vse reagente za pripravo, tudi vodo brez vsebnosti mikrobne DNA.

Testiranje Hrane: Perutnina

Testiranje Hrane: Morska hrana

Testiranje Hrane: Zelenjava

Testiranje Hrane: Mlečni izdelki

Testiranje Hrane: Meso

Vaginalna flora

Bakterijska Vaginoza

Okužbe sečil

Črevesne okužbe

Presnovne motnje

Okužbe dihal

Ustne bolezni

Sepsa

Odpornost na antibiotike

Analiza vode

Biološka obramba

Slika: Primer plošče Microbial DNA qPCR Arrays. Na plošči testiramo en vzorec z 90 Microbial testi. Vsaka jamica vsebuje posamezen test. Vključene so tudi kontrole vzorca, pan-bakterijska, pan-glive in pozitivna PCR kontrola

Microbial DNA qPCR Multi-Assay Kits (330043)

Kompletni kit za mikrobno profiliranje s kontrolami in reagenti

Vsebuje ploščo z experimentalno preverjenimi Microbial DNA qPCR aseji.

Uporablja se 5' hidrolizirano sondo za občutljivo in specifično detekcijo

Microbial DNA qPCR aseji, Microbial DNA Positive Control, Positive PCR Control, Microbial DNA-Free Water, and

Microbial qPCR Mastermix.

Bartonella henselae Pathogenicity Mycobacterium spp Pathogenicity

Bordetella pertussis Pathogenicity Neisseria meningitidis Pathogenicity

Brucella spp Pathogenicity Pseudomonas aeruginosa Pathogenicity

Campylobacter jejuni Pathogenicity Salmonella enterica Pathogenicity

Chlamydia trachomatis Pathogenicity Shigella dysenteriae Pathogenicity

Clostridium difficile Pathogenicity Streptococcus agalactiae Pathogenicity

Clostridium perfringens Pathogenicity Streptococcus pneumoniae Pathogenicity

C. diphtheriae Pathogenicity Streptococcus pyogenes Pathogenicity

Enterococcus faecalis Pathogenicity Vibrio cholerae Pathogenicity

Enterohaemorrhagic E. coli Pathogenicity Yersinia enterocolitica Pathogenicity

Haemophilus influenzae Pathogenicity Yersinia spp Pathogenicity

Helicobacter pylori Pathogenicity MRSA: Methicillin-resistant S. aureus

Legionella pneumophila Pathogenicity Laboratory Rodent Testing

Listeria monocytogenes Pathogenicity SHV Antibiotic Resistance Genes

Sestava Microbial DNA qPCR Multi-Assay Kita, MRSA: Methicillin-resistant S. aureus

Microbial DNA-Free Water: 338132; Microbial DNA Positive Control: 338134; Supplemental Microbial qPCR Mastermix (Fluorescein): 330540; Supplemental Microbial qPCR Mastermix (ROX): 330530;

BLU-V Viability PMA Kit 296015, 9002300 Za diferenciacijo živih in mrtvih mikroorganizmov v PCR reakcijah

Akcija velja do 15.07. 2015.

Tehnične informacije

Limit of detection versus lower limit of quantification.

This chart demonstrates the difference between the limit of detection (LOD) and the lower limit of quantification (LLOQ). The LOD is

defined as the lowest concentration at which 95% of the positive

samples are detected, whereas the LLOQ is the lowest concentration that falls within the linear range of a standard curve. LOD depends

upon the precision of the assay, and requires at least 40 replicates for

determination of a positive sample. For the Microbial DNA qPCR Assays, LLOQ is sufficient to determine assay sensitivity.

Linearity and sensitivity of Microbial DNA qPCR Arrays.

Linearity and sensitivity for each Microbial DNA qPCR Array was determined using synthetic templates over a 6 log serial dilution ranging from 1 copy to 1 million copies.

The following are representative results for all the qPCR assays. [A] shows the real-

time amplification curves of the KPC antibiotic resistance gene qPCR assay. In [B], a standard curve was prepared that shows that the primer efficiency equals 103%

(calculated from slope = –3.3236) and the correlation coefficient is 0.9983, indicating

optimum performance for the KPC qPCR assay. All Microbial DNA qPCR Assays have primer efficiencies between 80–120% and correlation coefficients (R)>0.995.

The lower limit of quantification

(LLOQ) for all Microbial DNA qPCR

Assays reveals high sensitivity.

This chart shows the distribution of LLOQ for all Microbial DNA qPCR Assays. 93% of all

Microbial DNA qPCR Assays have a LLOQ of

<100 gene copies.

The lower limit of quantification

(LLOQ) for antibiotic resistance gene detection Microbial DNA qPCR

Assays reveals high sensitivity.

This chart shows the distribution of LLOQ for Microbial DNA qPCR

Assays for antibiotic resistance gene

detection. 95% of all antibiotic resistance gene assays have a LLOQ

of <100 gene copies.

The lower limit of quantification

(LLOQ) for microbial identification Microbial DNA qPCR Assays reveals

high sensitivity.

This chart shows the distribution of LLOQ for microbial identification

Microbial DNA qPCR Assays. 92%

of all microbial identification assays have a LLOQ of <100 gene copies.

The lower limit of quantification

(LLOQ) for virulence factor gene detection Microbial DNA qPCR

Assays reveals high sensitivity.

This chart shows the distribution of LLOQ for Microbial DNA qPCR

Assays for virulence factor gene

detection. 97% of all virulence factor gene assays have a LLOQ of <100

gene copies

Microbial DNA qPCR Assays display high sensitivity even in complex metagenomic samples.

To ensure that Microbial DNA qPCR Assays performed comparably in a complex sample, where there

may be up to a thousand different microbial species, each assay was tested using stool, tooth plaque, and sputum samples. For each sample, synthetic template targets were spiked in and the CT was compared to

synthetic template alone. PCR was performed using several sample types, which included pooled synthetic

template targets alone, stool, stool plus pooled synthetic template targets, plaque, plaque plus pooled synthetic template targets, sputum, and sputum plus pooled synthetic template targets. If the CT<35 in

stool, plaque, or sputum samples alone, then ΔCT was calculated (i.e., CTstool + pooled synthetic template

targets – CTpooled synthetic template targets). This calculation was performed for all the assays. For each assay, the ΔCT<3, indicating that a complex metagenomic background does not affect the performance of

each Microbial DNA qPCR Assay.

Vaginal samples positive for Gardnerella vaginalis also show changes in commensal and bacterial vaginosis-related

microbes compared to healthy samples.

To compare any differences in the vaginal microbiome between healthy women and women with bacterial

vaginosis, each sample that tested positive for Gardnerella

vaginalis using the Vaginal Flora Microbial DNA qPCR Array was compared to samples from healthy women

(n=3). Fold-change in microbial species abundance was

calculated by the ΔΔCT method using human genomic

DNA to normalize. The results show that as the relative

abundance of Gardnerella vaginalis increases, the abundance of the commensal species Lactobacillus

crispatus decreases. Also, an increase in Gardnerella

vaginalis was associated with an increase in other bacterial vaginosis-associated microbial species. This suggests that

Lactobacillus crispatus protects the vagina from bacterial

vaginosis-associated microbial species.

Specificity of the Antibiotic Resistance Genes Microbial DNA qPCR Array is verified by pyrosequencing.

To verify the specificity of the Antibiotic Resistance Genes Microbial DNA

qPCR Array (cat no. BAID-1901Z) results from Klebsiella pneumoniae isolates, pyrosequencing assays were designed to detect for the presence and

sequences of SHV-156G, SHV-156D, SHV-238G240E, SHV-238S240K,

SHV-238S240E, SHV-238G240K, ermB, mefA, tetA, tetB,CTX-M-1 Group, CTX-M-2 Group, AAC(6′)-lb-cr and aadA1. For each Klebsiella pneumoniae

isolate, results from the Antibiotic Resistance Genes Microbial DNA qPCR

Array were confirmed by pyrosequencing. Representative pyrograms for [A] SHV-156G, [B] SHV-238/240, [C] KPC and [D] CTX-M-1 group are shown.

For SHV variants, the Antibiotic Resistance Gene Microbial DNA qPCR

Array was able to reliably distinguish single nucleotide polymorphisms

occurring at different sites.

The Vaginal Flora Microbial DNA qPCR Array provides accurate profiling for cervical swab samples.

The vaginal microbiota is a key component influencing women’s urogenital

health. To determine what changes in the vaginal microbiota occurs during bacterial vaginosis, the Vaginal Flora Microbial DNA qPCR Array (cat. no.

BAID-1902Z), which detects up to 90 different microbial species, was used to

test cervical swabs from healthy individuals and from patients with bacterial vaginosis. Genomic DNA from vaginal samples originating from three

patients that tested negative for bacterial vaginosis, three patients that tested

positive for Candida, three patients that tested positive for Garderella vaginalis, and one patient that tested positive for Trichomonas vaginalis by

BD Affirm™ VPIII Microbial Identification Test were run on the Vaginal

Flora Microbial DNA qPCR Array. Genomic DNA from ThinPrep samples

were isolated using QIAGEN’s QIAamp MinElute Media Kit and 500 ng

genomic DNA from each sample was analyzed. After the PCR run on a Roche

LightCycler 480, raw CT values were exported to the Microbial DNA qPCR data analysis software. Positive (+) / negative (blank) / inconclusive (+/-)

results for each microbial species were determined using the identification

criteria. The results from the Vaginal Flora Microbial DNA qPCR Array were in concordance with the BD Affirm VPIII Microbial Identification Test.

The Microbial DNA qPCR Array screens gut microbiota for the presence of antibiotic resistance genes.

The human gut microbiota is known to act as a reservoir for antibiotic resistance genes, where they can be transferred horizontally to potential pathogenic

bacteria. To detect the presence of antibiotic resistance genes from gut microbiota, stool samples from five healthy adults were collected and genomic DNA was

isolated using QIAGEN’s QIAamp DNA Stool Mini Kit. 500 ng genomic DNA from each stool sample was analyzed for presence of antibiotic resistance genes using the Antibiotic Resistance Genes Microbial DNA qPCR Array (cat. no. BAID-1901Z). The Antibiotic Resistance Genes Microbial DNA qPCR Array

contains assays for 83 antibiotic resistance genes, assays to identify methicillin-resistant Staphylococcus aureus, and control assays. ErmB, mefA, and tetA

were found in all or most of the stool samples tested, showing that they may be highly prevalent in the gut. These antibiotic resistance genes have been reported to be isolated from bacterial strains originating from food, suggesting a possible source of origin. This highlights the importance of increased monitoring of

antibiotic resistance reservoirs to identify potential sources of antibiotic-resistant bacteria.