MICRO TOTAL ANALYSIS SYSTEMS 2002

Micro Total Analysis Systems 2002

Proceedings of the IlTAS 2002 Symposium, held in N ara, Japan, 3-7 November 2002

Volume 2

edited by

Yoshinobu Baba The University of Tokushima, Tokushima, Japan

Shuichi Shoji Waseda University, Tokyo, Japan

and

Albert van den Berg University ofTwente, Enschede, The Netherlands

SPRINGER-SCIENCE+BUSINESS MEDIA, RV,

Library of Congress Cataloging-in-Publication Data.

ISBN 978-94-010-3953-6 ISBN 978-94-010-0504-3 (eBook) DOI 10.1007/978-94-010-0504-3

Printed on acid-free paper

All Rights Reserved © 2002 Springer Science+Business Media Dordrecht Originally published by Kluwer Academic Publishers in 2002 Softcover reprint of the hardcover 1 st edition 2002 No part of this work may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, microfilming, recording or otherwise, without written permission from the Publisher, with the exception of any material supplied specifically for the purpose of being entered and executed on a computer system, for exclusive use by the purchaser of the work.

PROGRAM COMMITTEE IJTAS2002

Yoshinobu Baba University of Tokushima

David Beebe University of Wisconsin-Madison

Albert van den Berg MESA + Research Institute, University of Twente

D. Jed Harrison University of Alberta

Klavs F. Jensen Massachusetts Institute of Technology

Thomas Laurell Lund Institute of Technology, Lund University

Andreas Manz Imperial College of London

M. Allen Northrup Microfluidic Systems Inc.

J. Michael Ramsey Oak Ridge National Laboratory

Shuichi Shoji Waseda University Takehiko Kitamori University of Tokyo Sabeth Verpoorte

University of NeucMtel

PREFACE

The Sixth International Conference on Miniaturized Chemical and Biochemical Analysis Systems, known as IlTAS2002, will be fully dedicated to the latest scientific and technological developments in the field of miniaturized devices and systems for realizing not only chemical and biochemical analysis but also synthesis. The first IlTAS meeting was held in Enschede in 1994 with approximately 160 participants, bringing together the scientists with background in analytical and biochemistry with those with Micro Electro Mechanical Systems (MEMS) in one workshop. We are grateful to Piet Bergveld and Albert van den Berg of MESA Research Institute of the University of Twente for their great efforts to arrange this exciting first meeting. The policy of the meeting was succeeded by late Prof. Dr. Michael Widmer in the second meeting, IlTAS'96 held in Basel with 275 participants. The first two meetings were held as informal workshops. From the third workshop, IlTAS'98 (420 participants) held in Banff, the workshop had become a worldwide conference. Participants continued to increase in IlTAS2000 (about 500 participants) held in Enschede and IlTAS2001 (about 700 participants) held in Monterey. The number of submitted papers also dramatically increased in this period from 130 in 1998, 230 in 2000 to nearly 400 in 2001. From 2001, IlTAS became an annual symposium. The steering committee meeting held in Monterey, confirmed the policy of former IlTAS that quality rather than quantity would be the key-point and that the parallel-session format throughout the 3.5 days would be maintained. The upcoming IlTAS2002 is the first meeting specialized on this field that is held in Asia. Again there is a very large increase in submitted abstracts (over 450), especially from the Asian Countries such as Japan, Korea, Taiwan, and China. The program committee had a very hard work to do and had to select 66 abstracts as oral presentations and more than 250 abstracts as poster presentations, an acceptance rate slightly over 70 %. In addition, we have excellent invited speakers from North America, Europe and Asia. The co-chairs of IlTAS2002 wish everyone will understand the consistent IlTAS policy and agree that the program committee did a very excellent job in selecting abstracts and arranging the program. This is the first IlTAS meeting in Asia, we do hope the meeting will contribute to spread the latest knowledge of IlTAS to Asia as well as North America and Europe.

Recent trend that appeared in the previous IlTAS2001 is that the contributions are much more focused on the actual applications than on the basic studies. Microfluidc components, devices and integrated systems are still a main stream in the MEMS field. A droplet base fluidics, nanofluidics and a gas-liquid two phase flow are the new topics. Integration of the flow devices for a total system is obvious. Plastic micro fabrication, using PDMS are currently used and miniaturization technologies proceeds to realize the structure of nanometer scale. The interest in materials for biochemical and biomedical applications and nanotechnologies is showing remarkable growth. The genomics and proteomics analysis and separation science remain high from the previous meeting. The

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interests of the clinical diagnosis and cellular analysis have considerably increased. Devices and systems developments that address highly sensitive measurements of chemical and biochemical molecules continued in the micro-optics, mass spectroscopy and electrochemical detection.

We would like to express our great gratitude to program committee members of the 12 steering committee members and Takehiko Kitamori for their really tough work. We would like to greatly thank D. Jed Harrison, Albert van den Berg and J. Mike Ramsey for sharing their experiences with previous meetings and providing many useful suggestions. We also thank Hiroyuki Fujita, Takehiko Kitamori and other local organizing committee members. The excellent arrangement could not be done without the efforts by June Echizen of the secretariat of IlTAS2002 and her colleagues. Finally we wish to thank the most important people of all, the invited speakers, the technical contributors and participants! We hope all participants will enjoy IlTAS2002.

Yoshinobu Baba Shuichi Shoji IlTAS2002 Co-Chairman August 10, 2002

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VOLUME 1 Day 1 Keynote 1

CONTENTS

EXPLORING FET CONCEPTS FOR LAB-ON-A-CHIP ............................................. 1

P. Bergveld Cell Growth and Monitoring MONITORING OF CELL GROWTH, OXYGEN AND pH IN MICROFERMENTORS .................................................................................................. 7

Nicolas Szita, Andrea Zanzotto, Paolo Boccazzi, Anthony J. Sinskey, Martin A. Schmidt and Klavs F. Jensen OBSERVATION OF RESPONSE TO STIMULI IN OSCILLATING CELLS PATTERNED BY MICROFABRICATED STRUCTURE ........................................... 10

Atsuko Takamatsu and Teruo Fujii ON-CHIP AGAROSE MICROCHAMBER (AMC) ARRAY CELL-CULTIVATION SYSTEM FOR TOPOGRAPHICAL CONTROL OF NEURAL NETWORK ............ 13

Hiroyuki Moriguchi, Kazunori Takahashi, Tomoyuki Kaneko and Kenji Yasuda Separation, General INTEGRATED SIZE EXCLUSION AND REVERSED-PHASE ELECTROCHROMATOGRAPHY .............................................................................. 16

Abebaw B. Jemere, Richard D. Oleschuk and D. J. Harrison DISPERSION ANALYSIS OF A SOLID-PHASE MICROEXTRACTION CHIP BY COMPUTATIONAL FLUID DYNAMICS .................................................................. 19

Jonas Bergkvist, Simon Ekstrom, Johan Nilsson and Thomas Laurel! INTERFACING MICROCHIP CE WITH ICPMS FOR ELEMENT SPECIATION ................................................................................................................ 22

Qi Jun Song, Gillian M. Greenway and Tom McCreedy Gels for Biochemical Analysis ELECTRODELESS DIELECTROPHORETIC TRAPPING AND SEPARATION OF CELLS ........................................................................................................................... 25

Chia-Fu Chou, Margaret Morgan, Frederic Zenhausern, Christelle Prinz and Robert H. Austin Si02 NOZZLE ARRAY-BASED PATCH-CLAMP MICROSYSTEM ....................... 28

T. Lehnert, R. Netzer, U. Bischoff and M. A. M. Gijs A PROGRAMMABLE CELL ASSAY PLATFORM FOR KINETIC STUDIES OF A SINGLE CELL .............................................................................................................. 31

Antoine Daridon, William Throndset, Ian Liau, Kevin Farrel!, Flora Tseng, Shervin Javadi and Ian Manger

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Micro Analysis Systems AN INTEGRATED MICRODIALYSIS-BASED SYSTEM ........................................ 34

D. G. Pijanowska, A. l.Sprenkels, l. Bomer, W. Olthuis and P. Bergveld USER-ASSEMBLY, FULLY INTEGRATED MICRO CHEMICAL LABORATORY USING BIOCHEMICAL IC CHIPS FOR WEARABLEIIMPLANTABLE APPLICATIONS ........................................................................................................... 37

Koji Ikuta, Atsushi Takahashi, Kota Ikeda and Shoji Maruo MICROFLUIDIC REACTOR ARRAY FOR MULTISTEP DROPLET REACTIONS ................................................................................................................. 40

Masumi Yamada, Nobuyuki Sugiyama and Minoru Seki Keynote 2 SINGLE MOLECULAR DETECTION OF DNAJPROTEIN AND ITS APPLICATION TO BIOCHIPS .................................................................................... 43

Tomoji Kawai DNA Separation DNA SEPARATION IN GRADIENT OF CONFINED NANOSPACE GENERATED BY NANOPARTICLES IN A MICROCHANNEL.. .................................................... 45

Noritada Kaji, Mari Tabuchi, Yukio Nagasaki, Kazunori Kataoka, Kenichi Yoshikawa and Yoshinobu Baba SELF-ASSEMBLED MAGNETIC COLLOIDS FOR DNA SEPARATIONS IN MICROFLUIDIC DEVICES ........................................................................................ 48

Patrick S. Doyle, Claus Filtterer, Nicolas Mine, Cecile Goubault, Jerome Bibette and lean-Louis Viovy A MICROFABRICATED DEVICE FOR SEPARATING -200KILO-BASE-PAIR DNA MOLECULES IN -15 SECONDS ...................................................................... 51

Lotien Richard Huang, Jonas O. Tegenfeldt, lames C. Sturm, Robert H. Austin and Edward Cox Droplet Base Fluidics AIRBORN CHEMISTRY LEVITATED PROTEIN DROPLETS AS A NOVEL ANALYTICAL TOOL FOR NUCLEATION SCREENING IN MACROMOLECULAR CRYSTALLISATION ........................................................... 54

Sabina Santesson, Eila S. Cedergren-Zeppezauer, Thomas lohansson, Thomas Laurell, lohan Nilsson and Staffan Nilsson ON-LINE MONITORING OF AIRBORNE CHEMISTRY IN LEVITATED DROPLETS: IN-SITU SYNTHESIS AND APPLICATION OF SERS ACTIVE Ag-SOLS FOR TRACE ANALYSIS BY RAMAN SPECTROMETRy ..................... 58

Nicolae Leopold, Michael Haberkorn, Thomas Laurell, Johannes Nielson, losefa R. Baena and Bernhard Lendl

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CHIP-TO-WORLD INTERFACES FOR HIGH-THROUGHPUT LAB-ON-A-CHIP DEVICES ...................................................................................................................... 61

Sebastian Bohm, Theo Veenstra, Albert van den Berg, Nghia Chiem and John Gilbert Day 1 Posters Fluid Mechanics & Design Tools THE MEMS MODELING SYSTEM BY COLLABORATION OF MULTI-SCALE SIMULATORS AND APPLICATION TO THE MICROREACTOR .......................... 64

Tatsuya Yamaue, Taku Ozawa, Eisuke Nishitani, Makoto Sasaki, Shinji Shibano, Takashi Taniguchi, Yuichi Masubuchi and Masao Doi FEM STUDY OF COULTER COUNTER WITH WATER-BASED ADAPTABLE APERTURE ................................................................................................................... 67

J. H. Nieuwenhuis and M. J. Vellekoop MICROMANIPULATING MAGNETIC PARTICLES IN MICROFLUIDIC SySTEMS ..................................................................................................................... 70

E. Brunet, B. Michaud, F. Okkels and P. Tabeling OPTIMAL DESIGN OF MICROMIXER USING NONHOMOGENEOUS MULTILAYER LAMINAR FLOW .............................................................................. 73

Masaki Kanai, Daisuke Uchida, Satoshi Sugiura, Hironobu Sato, Yoshikazu Mori, Katsumasa Sakamoto, Hiroaki Nakanishi and Shuichi Shoji COMPUTATIONAL SIMULATIONS OF FLUID FLOW DYNAMICS, AND BEAD PACKING IN SOLID PHASE EXTRACTION MICROSYSTEMS ........................... 76

Paul D. Senkans, Vern de Biasi and David A. Barrow THE LOW-VOLTAGE CASCADE EOF PUMP: COMPARING THEORY WITH PUBLISHED DATA ...................................................................................................... 79

Anders Brask, Goran Goranovif: and Henrik Bruus UNIVERSAL JOULE HEATING MODEL IN ELECTROPHORETIC SEPARATION MICROCHIPS ..................................................................................... 82

Yi Wang, Qiao Lin and Tamal Mukherjee MATHEMATICAL MODEL OF INTERFACIAL LAYER IN ULTRA-FINE LIQUID DROP BASED ON MOLECULAR DYNAMICS SIMULATION ............... 85

Satoyuki Kawano, Tomoyuki Shiga and Kazuhiro Nakanishi DEVELOPMENT OF MICROMIXER BASED ON INSTABILITY OF INTERFACE BETWEEN TWO IMMISCIBLE LIQUIDS .......................................... 88

Satoyuki Kawano, Hirofumi Shintaku, Isaku Kanno and Hidetoshi Kotera THEORETICAL STUDY OF AC ELECTROOSMOTIC FLOWS IN NON-UNIFORMLY CHARGED MICROCHANNELS .............................................. 91

Sung Jae Kim, Hyo Sook Cha, In Seok Kang and Byung Jun Yoon

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NUMERICAL SIMULATION OF MIXING IN A MICRO-CHANNEL WITH NON-UNIFORM ZETA POTENTIAL SURFACE ...................................................... 94

Seungbae Hong, Luc G. Frechette and Vijay Modi

INJECTION OF SAMPLE BANDS FROM OPEN CHANNELS INTO PACKED SEPARATION COLUMNS .......................................................................................... 97

Robert H. Nilson, Stewart K. Griffiths and Anup K. Singh MICROSCALE MEASUREMENTS OF FLOW BOUNDED BY AIR-WATER INTERFACES ............................................................................................................. 100

J. M. Bauer and D. J. Beebe VIRTUAL FLOW CHANNEL: A NOVEL MICRO-FLUIDICS SYSTEM WITH ORTHOGONAL, DYNAMIC CONTROL OF SAMPLE FLOW DIMENSIONS .... 103

J. H. Nieuwenhuis, J. Bastermeijer, P. M. Sarro and M. J. Vellekoop

Micro Machining Methods MICROFABRICATION OF 3-D OBLIQUE STRUCTURES BY INCLINED UV LITHOGRAPHy ......................................................................................................... 106

Manhee Han, Woonseob Lee, Sung-Keun Lee and Seung S. Lee

A NEW FABRICATION TECHNIQUE OF A DNA ELECTROPHORESISCHIP BY SILICON MICROMACHINING TECHNOLOGY ................................................... 109

J. Tsuda, Y. Tada, G. Hashiguchi, K. Hirano, N. Kaji and Y. Baba

PRECISION PATTERNING OF PDMS THIN FILMS: A NEW FABRICATION METHOD AND ITS APPLICATIONS ...................................................................... 112

Kee Suk Ryu and Chang Liu

FABRICATION OF MICROFLUIDIC CHANNELS WITH SYMMETRIC CROSS-SECTIONS FOR INTEGRATED NMR ANALySIS ................................... 115

A. Homsy, J. Lichtenberg, C. Massin, F. Vincent, P. -A. Besse, R. S. Popovic, N. F. de Rooij and E. Verpoorte PRECISE AND FEASIBLE FABRICATION PROCESS OF MICROFLUIDIC DEVICES ON A BOROSILICATE GLASS CHIP .................................................... 118

Y. Sugiyama, Y. Otsu, T. Ichiki and Y. Horiike

LAMINATED ELECTRODES CHIP FOR PULSE-IMMUNOASSAY .................... 121

Takeshi Ooi, Yusuke Okabe, Masayuki Nakao and Keisuke Iwata

FABRICATION OF ON-CHIP SORTER DEVICES WITH SUB-MICROMETER SCALE CHANNELS AND SELF-ALIGNED MICROELECTRODES ................... 124

T. Hara, T. Ichiki, Y. Horiike and K. Yasuda

POWDER HANDLING DEVICE FOR DRUG FORMULATIONS ......................... 127

Torsten Vilkner and Andreas Manz

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Micropumps & Microvalves THERMAL MICROPUMP USING SURFACE TENSION IMBALANCE IN MICROCHANNELS ................................................................................................... 130

Koji Takahashi, Hitoshi Ikeda, Tatsuya Ikuta, Kunihito Nagayama and Tanemasa Asano OPTIMIZATION OF ELECTROKINETIC PUMPS FOR CHIP-BASED CHROMATOGRAPHIC SEPARATIONS .................................................................. 133

S. K. Griffiths and R. H. Nilson PRACTICAL VALVES AND PUMPS FOR LARGE-SCALE INTEGRATION INTO MICROFLUIDIC ANALYSIS DEVICES .................................................................. 136

William H. Grover, Alison M. Skelley, Chung N. Liu, Eric T. Lagally and Richard A. Mathies ELECTRO OSMOTIC FLOW PUMP BASED ON THE CORBINO DISC GEOMETRY ............................................................................................................... 139

Jonatan Kutchinsky, Monica Backes, Stephen Dennison, Ian Gibb, Claus B.SBrensen, Ras Kaas Vestergaard, John Dodgson, John Shaw and Rafael Taboryski A TEMPERATURE CONTROLLED MICRO VALVE FOR BIOMEDICAL APPLICATIONS USING A TEMPERATURE SENSITIVE HYDROGEL .............. 142

Heiko J. van der Linden, David J. Beebe and Piet Bergveld ELECTROOSMOTICALLY DRIVEN TWO-LIQUID VISCOUS PUMP FOR NONCONDUCTING LIQUIDS ................................................................................. 145

Anders Brask, Goran Goranovic and Henrik Bruus BI-DIRECTIONAL VALVE-LESS MICROPUMP USING PIEZOELECTRIC MICRO-ARRAY ACTUATORS ................................................................................ 148

Shunsuke Yakushiji, Isaku Kanno and Hidetoshi Kotera GRAVITY-DRIVEN MICROPUMP WITH A STEADY FLOW RATE ................... 151

Xiaoyue Zhu, Nandita Phadke, Joseph Chang, Brenda Cho, Dongeun Huh and Shuichi Takayama A MICRO PUMP, COMPATIBLE WITH MULTIPLE MANUFACTURING METHODS, FOR FLUIDHANDLING IN DISPOSABLE MICROSYSTEMS ....... 154

M. Schlilter, U. Kampmeyer, A. Hermsdorf and H. J. Lilienhof MICROFABRICATION PROCESS FOR HIGH-DENSITY MICRO PIPETTE ARRAY AND MATCHING MULTI-WELL PLATE WITH MIXERS ..................... 157

Kee Suk Ryu, ZhiJang Fan and Chang Liu MICROFABRICATED PHASE CHANGE LATCHED VALVE FOR INTEGRATED DEVICES .................................................................................................................... 160

Rohit Pal and Mark Burns THERMAL-ACTUATED PARAFFIN MICROVALVES .......................................... 163

Robin H. Liu, Justin Bonanno and Piotr Grodzinski

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INJECTION MODES FOR EVAPORATION DRIVEN CONTINUOUS LIQUID FLOW .......................................................................................................................... 166

N. Goedecke and A. Manz

Clinical Diagnosis DNA AMPLIFICATION AND HYBRIDIZATION ASSAYS IN INTEGRATED PLASTIC MONOLITHIC DEVICES ......................................................................... 169

Yingjie Liu, Cory B. Rauch, Randall L. Stevens and Piotr Grodzinski MICROFLUIDIC DEVICE FOR NUCLEIC ACID FRAGMENTATION BY SHEAR FORCE ........................................................................................................................ 172

Jan Lichtenberg, Laura Ceriotti, Nathan A. Lacher, Susan M. Lunte, Nico F. de Rooij and Elisabeth Verpoorte COMBINED NUCLEIC ACID EXTRACTION AND ENRICHMENT IN BEAD-PACKED PLASTIC BEDS ............................................................................. 175

Laura Ceriotti, Jan Lichtenberg, Arash Dodge, Nico de Rooij and Elisabeth Verpoorte MEASURING LITHIUM IN WHOLE BLOOD USING CAPILLARY ELECTROPHORESIS ................................................................................................ 178

Elwin Vrouwe, Regina Luttge and Albert van den Berg AN INGESTIBLE ELECTRONIC PILL FOR REAL TIME ANALYTICAL MEASUREMENTS OF THE GASTRO-INTESTINAL TRACT .............................. 181

Erik A. Johannessen, Tong-Boon Tang, Lei Wang, Lili Cui, Mansour Ahmadian, Nizamettin Aydin, Alexandros Astaras, Alan F. Murray, Brian W. Flynn, Tugrul Asian, Steve P. Beaumont, David R. S. Cumming and Jonathan M. Cooper DETECTION OF MULTIPLE REAL-TIME NASBA IN NANOLITER VOLUME .................................................................................................................... 184

Anja Gulliksen, Lars Solli, Frank Karlsen, Reidun Sirevag and Henrik Rogne DISPOSABLE BIOCHIP CARTRIDGE FOR CLINICAL DIAGNOSTICS TOWARD POINT-OF-CARE SYSTEMS .................................................................. 18 7

Chong H. Ahn, Jin-Woo Choi, Aniruddha Puntambekar, Chien-Chong Hong, Xiaoshan Zhu, Chuan Gao, Ramachandran Trichur, Srikanth Chilukuru, Madhulika Dutta, Suresh Murugesan, Sanghyo Kim, Young-Soo Sohn, Joseph H. Nevin, Gregory Beaucage, Jeong-Bong Lee, Jae Y. Lee and Michael G. Bissell MICROCHIP-BASED ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) SySTEM ..................................................................................................................... 190

Kiichi Sato, Maho Yamanaka, Manabu Tokeshi, Keisuke Morishima and Takehiko Kitamori INFRARED-MEDIATED THERMOCYCLING FOR DNA AMPLIFICATION AND ELECTROPHORETIC SEPARATION ON AN INTEGRATED MICROCHIP DEVICE ...................................................................................................................... 193

Yien C. Kwok, Braden C. Giordano, Jerome P. Ferrance and James P. Landers

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INTEGRATED MICRODEVICE FOR DNA EXTRACTION FROM A SINGLE HAIR ........................................................................................................................... 195

Nae Yoon Lee, Masumi Yamada and Minoru Seki MONOLITHIC SOL-GEL MICROCHIP DEVICE FOR EFFICIENT ISOLATION OF NUCLEIC ACID FROM CLINICAL SAMPLES ................................................ 198

Qirong Wu, Michael C. Breadmore, Benjamin J. Hassan, Barden C. Giordano, Yien C. Kwok, James M. Karlinsey, Jerome P. Ferrance, Sushil Shrinivasan, Pamela A. Norris and James P. Landers MINIATURIZATION OF A HEMATOLOGY ANALYZER FOR MONITORING ONCOLOGY OUTPATIENTS ................................................................................... 201

Ulrik Darling Larsen and Bjorn Ekberg FABRICATIONS AND APPLICATIONS OF MICRO HEMOCYTOMETER FOR EARLY DETECTION OF CANCER ......................................................................... 204

Junha Park, Seok Chung, Chanil Chung, Dong Chul Han and Jun Keun Chang ENABLING TECHNOLOGIES FOR A PERSONAL FLOW CYTOMETER, PART II: INTEGRATED ANALYSIS CARTRIDGES ......................................................... 207

Jon Hayenga, Ron Bardell, Chris Morris, Paul Graham, Natasa Kesler, Christy Lancaster, Aravind Padmanabhan, Cleopatra Cabuz, Tom Schulte, Bernhard H. Weigl, Patrick Saltsman, Mingchao Shen and C. Fred Battrell HIGH THROUGHPUT STRESS MARKER ASSAY USING POLYMER MICROCHIP ELECTROPHORESIS WITH LASER INDUCED FLUORESCENCE DETECTION ............................................................................................................... 210

Shin-ichi Wakida, Xiaoling Wu, Kyoko Akama, Tomoko Motoshige, Kohzoh Yoshino, Katsunori Matsuoka and Etsuo Niki Genomics and Proteomics DNA SIZE SEPARATION EMPLOYING MICRO-FABRICATED MONOLITHIC NANO-STRUCTURE ................................................................................................. 212

Yojiro Tezuka, Masanori Ueda, Yoshinobu Baba, Hiroaki Nakanishi, Takahiro Nishimoto, Yuzuru Takamura and Yasuhiro Horiike INTEGRATED MICROSYSTEM OF ISOTHERMAL AMPLIFICATION OF DNA AND ELECTROPHORESIS ON A MICROFABRICATED PLASTIC CHIP FOR DETECTION OF SPECIFIC GENE AND ANALYSIS OF GENETIC MATERIALS ............................................................................................................... 215

Yukari Hataoka, Tsugunori Notomi and Yoshinobu Baba SNP ANALYSIS BY ALLELE-SPECIFIC EXTENSION OF FLUORESCENTLY LABELED NUCLEOTIDES IN A MICROFLUIDIC FLOW-THROUGH DEVICE ...................................................................................................................... 218

Aman Russom, Helene Andersson, Peter Nilsson, A/shin Ahmadian and Goran Stemm

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DEVICE FOR LABEL-FREE BIO-LIGAND INTERACTION STUDIES BASED ON TIME RESOLVED FOURIER TRANSFORM INFRARED SPECTROMETRY ...................................................................................................... 221

B. Lendl, P. Hinsmann, P. Svasek and J. Frank A DISPOSABLE PASSIVE MICROFLUIDIC SYSTEM INTEGRATED WITH MICROMIXER AND DNA PURIFICATION CHIP FOR DNA SAMPLE PREPARATION .......................................................................................................... 224

Joon-Ho Kim, Byoung-Gyun Kim, Munho La, Jun-Bo Yoon and Euisik Yoon

DNA HYBRIDIZATION FEATURE ON "BEAD-ARRAY" - DNA PROBES ON BEADS ARRAYED IN A CAPILLARy .................................................................... 227

Yoshinobu Kohara, Hideyuki Noda, Kazunori Okano and Hideki Kambara DEVELOPMENT OF A LABEL-FREE PROTEIN ARRAY CHIP .......................... 230

James K. Kariuki, Vishal Kanda, Mark T. McDermott and D. Jed Harrison DETECTION OF DNA POINT MUTATIONS IN A POLYMER MICROFLUIDIC NETWORK USING A TEMPORAL THERMAL GRADIENT ................................ 233

Jesse S. Buch, Frederick Rosenberger, Don DeVoe and Cheng S. Lee MICRO MULTIPLE IMMUNOASSAY USING SILICON-BASED MICROFABRICATED PROTEIN BIOCHIP ............................................................. 236

Mitsuo Isomura, Chisato Okamura, Nobuyuki Ise, Takashi Shirakawa and Yoshihiro Ashihara AN ACTIVE MIXER FOR MICROSCALE PURIFICATION AND SEQUENCING REACTION CLEAN-UP ............................................................................................ 239

Ling-Sheng Jang, Deirdre R. Meldrum and Mark R. Holl IN VITRO PROTEIN SYNTHESIS ON A HIGH-INTEGRATED MICROCHAMBER CHIP WITH LOW DNA MOLECULES .................................. 242

Takeshi Kinpara, Yasutaka Morita, Hideo Nakano, Tsuneo Yamane and Eiichi Tamiya PARALLEL SEQUENCING OF REAL-WORLD SAMPLES USING A MICROFABRICATED HYBIRD DEVICE ................................................................ 245

Shaorong Liu MICROMACHINED DEVICE FOR MANIPULATION OF CHARGED BIO-MOLECULE ....................................................................................................... 248

Jae Y. Yun, Sang J. Moon, Hong K. Nam, Seung S. Lee and Yeun T. Chi

HIGH PRECISION MICROPREPARATIVE SEPARATION SYSTEM BASED ON PLASTC MICROFLUIDIC MODULE - CAPILLARY COUPLING ....................... 251

Julia Khandurina and Andras Guttman

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Micro-Optical Systems HIGH FIDELITY AND LOW COST DETECTION OF MULTI-COLOR FLUORESCENCE FROM BIOLOGICAL CELLS IN A MICRO INTEGRATED FLOW CYTOMETER (MIFC) WITH DISPOSABLE OBSERVATION CELL ....... 254

Y. -G. Tung, G. -T. Lin, K. Kurabayashi and S. J. Skerlos

INTEGRATED AMORPHOUS SILICON PHOTODIODE DETECTOR FOR MICROFABRICATED CAPILLARY ELECTROPHORESIS DEVICES ................. 257

Toshihiro Kamei, James R. Scherer, Brian M. Paegel, Alison M. Skelley, Robert A. Street and Richard A. Mathies

INTEGRATION OF POLYMER WAVEGUIDES FOR OPTICAL DETECTION IN BIOCHEMICAL MICROSYSTEMS ......................................................................... 260

J. El-Ali, K. B. Mogensen, l. R. P. Nielsen, J. P. Kutter, P. Telleman and A. Wolff REAL-TIME SPR IMAGING OF BIOCHEMICAL REACTIONS IN MICROFLUIDIC SYSTEMS ..................................................................................... 263

Osamu Niwa, Yuzuru Iwasaki, Tatsuya Tobita, Kazuyoshi Kurihara and Koji Suzuki

A MINIATURIZED GLOW DISCHARGE OPTICAL EMISSION DETECTOR FOR AQUEOUS ANALYTES ............................................................................................ 266

Gareth Jenkins and Andreas Manz FIBER-OPTIC MICRODEVICES FOR SURFACE PLASMON RESONANCE APPLIED TO MICROFLUIDIC DEVICES ............................................................... 269

Kazuyoshi Kurihara, Yuzuru Iwasaki, Osamu Niwa, Tatsuya Tobita, Takeshi Ito and Koji Suzuki

OPTICAL RECOVERY OF PARTICLES ON A CHIP TOWARD CELL SORTING AND BEAD-BED DETECTION ................................................................................ 272

Ken Hirano and Yoshinobu Baba INTEGRATED ON-CHIP ABSORPTION AND LUMINESCENCE SPECTROSCOPY WITH POLYDIMETHYLSILOXANE (PDMS) MICROFLUIDIC FLOW CHANNELS USING BROADBAND ILLUMINATION 275 Mark L. Adams, Stephen R. Quake and Axel Scherer

DEVELOPMENT OF LIQUIDILIQUID OPTICAL WAVEGUIDE USING A TWO PHASE SHEATH FLOW AND ITS APPLICATION TO FLUORESCENT DETERMINATION OF RHODAMINE B ................................................................. 278

Hiromi Takiguchi, Tamao Odake, Tomonari Umemura and Kin-ichi Tsunoda

INTEGRATION OF PHOTO-THERMAL SPECTROSCOPY DETECTION SYSTEM ON A CHIP ................................................................................................ 281

J. Yamaguchi, A. Hattori, M. Tokeshi and T. Kitamori

INTEGRATED MICROFLUIDIC OPTICAL SYSTEMS (iMOS) WITH LED ........ 284

Jeonggi Seo and Luke P. Lee

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APPLICATION OF ORGANIC LIGHT EMITTING DIODE AS AN ON-CHIP LIGHT SOURCE FOR FLUORESCENCE SPECTROSCOPy ................................ 287

S. Camou, M. Kitamura, J-P. Gouy, H. Fujita, Y. Arakawa and T. Fujii MICRO-OPTICAL LASER INDUCED FLUORESCENCE DETECTION ON A MINIATURISED FLOW CYTOMETRY DEVICE ................................................... 290

Jan KrUger, Pier paolo Porta, Alan Morrison, Kirat Singh, Alan O'Neill and Peter O'Brien COMPARISON OF DIFFERENT STRATEGIES OF CHEMILUMINESCENCE DETECTION FOR MICROCHIP SYSTEM FABRICATED IN POLY(DIMETHYLSILOXANE) ............................................................................... 293

Bi-Feng Liu, Motoaki Ozaki, Shigeru Terabe, Yuichi Utsumi and Tadashi Hattori FIBER-LOOP RING-DOWN SPECTROSCOPY FOR ENHANCED DETECTION OF ABSORPTION WITH LIMITED PATH LENGTH ............................................. 296

Zhaoguo Tong, R. Stephen Brown, Hans-Peter Loock and Richard D. Oleschuk MULTICOMPONENT ANALYSIS BY SUBMILLIMETER-EFFECTIVE-LENGTH CAPILLARY ELECTROPHORESIS USING PATTERNED FLUORESCENCE CORRELATION SPECTROSCOPY .......................................................................... 299

Tsuyoshi Sonehara and Takashi Irie

Day 2 Plenary 1 SUPRAMOLECULAR AND MACROMOLECULAR APPROACHES TO FUNCTIONAL NANO MATERIALS ....................................................................... 302

TakuzoAida DNA Assay LABEL-FREE DIELECTRIC DETECTION OF DNA HYBRIDIZATION WITH NANOGAP JUNCTIONS ........................................................................................... 305

Joon Sung Lee, Sebaek Oh, Yang-Kyu Choi and Luke P. Lee DETECTION OF SINGLE NUCLEOTIDE INCORPORATION USING PYROSEQUENCING IN A MICROFLUIDIC DEVICE .......................................... 308

Aman Russom, Wouter van der Wijngaart, Hanno Ehring, Nigel Tooke, Helene Andersson and Goran Stemme FAST SCREENING OF SINGLE-NUCLEOTIDE POLYMORPHISMS USING CHIP-BASED TEMPERATURE GRADIENT CAPILLARY ELECTROPHORESIS ................................................................................................ 311

Peng Liu, Wan-Li Xing, Dong Liang, Guo-Liang Huang and Jing Cheng

xviii

Nano Fluidics MOLECULAR TRANSPORT THROUGH NANOMETER CONFINED CHANNELS ................................................................................................................ 314

J. Michael Ramsey, Jean Pierre Alarie, Stephen C. Jacobson and N. J. Peterson DNA TRAP-AND-RELEASE ELEMENT EMPLOING ELECTRIC AND HYDRO DRAG FORCE FIELDS FOR ON-CHIP PRE-TREATMENT .................................. 317

Yuzuru Takamura, Tetsuya Hayama, Masanori Ueda, Yoshinobu Baba and Yasuhiro Horiike MICROCHIP ELECTROPHORESIS INTEGRATED WITH NANOFLUIDIC SAMPLE HANDLING ............................................................................................... 320

Sang-Ho Lee, Seung Il Cho, Doo Soo Chung and Yong-Kweon Kim Magnetic MAGNETOPHORETIC-DIELECTROPHORETIC FIELD-FLOW FRACTIONATION ..................................................................................................... 323

Peter R. C. Gascoyne, Jody Vykoukal, Roy Weinstein, Alberto Gandini, Drew Parks and Ravi Sawh FREE-FLOW MAGNETOPHORESIS - SEPARATION OF MAGNETIC MICROPARTICLES AND AGGLOMERATES ON CHIP ....................................... 326

Nicole Pamme and Andreas Manz HYBRID TYPE ON-CHIP MAGNETIC PARTICLE SEPARATORS FOR ACCURATE POSITIONING MAGNETIC BEADS ................................................. 329

Jin- Woo Choi, Jaephil Do and Chong H. Ahn

Materials PHOTOPOLYMERIZED AND PHOTOGRAFTED POROUS POLYMER MONOLITHS FOR FABRICATION OF MICROFLUIDIC ANALYTICAL SYSTEMS ................................................................................................................... 332

Frantisek Svec, Jean M. J. Frechet, Emily F. Hilder, Dominic S. Peterson and Thomas Rohr ENGINEERED DIELECTRIC MICROSPHERES FOR USE IN MICROSYSTEMS ...................................................................................................... 335

Jody Vykoukal, Susan Sharma, Daynene Mannering Vykoukal and Peter R. C. Gascoyne MICROVALVE ARCHITECTURES FOR HIGH-PRESSURE HYDRAULIC AND ELECTROKINETIC FLUID CONTROL IN MICROCHIPS .................................... 338

Brian J. Kirby and Timothy J. Shepodd

Plenary 2 BIOPOEMS AND NANOGAP DNA JUNCTIONS .................................................. 341

Luke P. Lee

xix

Sample Prep System MULTIPLEXED PROTEIN PREPARATION SYSTEMS FOR PROTEOMICS ...... 344

Justine Taylor, Can Wang and D. Jed Harrison A NOVEL MINIATURIZED PROTEIN PRECONCENTRATOR BASED ON ELECTRIC FIELD-ADDRESSABLE RETENTION AND RELEASE .................... 347

Anup K. Singh, Daniel J. Throckmorton, Brian J. Kirby and Aidan P. Thompson DESIGN PRINCIPLES, PERFORMANCE AND PERSPECTIVES OF A COMPLETE MINIATURIZED ELECTROPHORETIC INSTRUMENT ................. 350

B. Stanislawski, D. Kaniansky, M. Masar and M. Johnck Microfluidics I GAS-LIQUID FLOWS IN MICROCHEMICAL SYSTEMS .................................... 353

Axel GUnther, Manish Jhunjhunwala, Nuria de Mas, Martin A. Schmidt and Klavs F. Jensen ON-CHIP CONCENTRATION OF LIQUID SAMPLES USING AN AIR-LIQUID TWO-PHASE FLOW .................................................................................................. 356

Manabu Tokeshi, Kazuteru Kanda, Akihide Hibara and Takehiko Kitamori TWO-PHASE FLOWS (OIUWATER): FLOW REGIMES AND WETTING .......... 359

Herve Willaime, Remi Dreyfus and Patrick Tabeling Day 2 Posters Microfluidics I PREPARATION OF PICOLITER-SIZED REACTION/ANALYSIS CHAMBERS FOR DROPLET-BASED CHEMICAL AND BIOCHEMICAL SYSTEMS ............ 362

Takasi Nisisako, Toru Torii and Toshiro Higuchi A MICRO-REACTOR EQUIPPED WITH SHEATH-FLOW INJECTORS FOR HIGH-SPEED EXTRACTION ................................................................................... 365

Tadashi Sano, Ryo Miyake, Akira Koide and Takeshi Harada MASS-PRODUCTION SYSTEM OF NEARLY MONODISPERSE DIAMETER GEL PARTICLES USING DROPLETS FORMATION IN A MICROCHANNEL .. 368

A. Kawai, T. Futami, H. Kiriya, K. Katayama and K. Nishizawa A MULTIPHASE LAMINAR FLOW DIFFUSION CHIP WITH ION SELECTIVE ELECTRODE DETECTION ...................................................................................... 371

Hong Chen, Qun Fang, Xue-Feng Yin and Zhao-Lun Fang

MICRO PIV MEASUREMENT OF ELECTROOSMOTIC FLOW .......................... 374

Haruyuki Kinoshita, Marie Oshima, Jong Wook Hong, Teruo Fujii, Tetsuo Saga and Toshio Kobayashi HIGH-EFFICIENCY SEPARATION IN MICRO FLUIDIC DEVICES FOR HIGH-THROUGHPUT SCREENING OF KINASES ............................................... 377

Bahram Fathollahi, Matthew B. Kerby, Spencer Wu and Ring-Ling Chien

xx

A MULTI-STAGE MICRO-TANGENTIAL FLOW FILTRATION DEVICE FOR MTAS APPLICATIONS .............................................................................................. 380

Patrik Leung, Leonggi Seo and Luke P. Lee MICROCONTACT SWITCHING OF SINGLE MOLECULES ................................ 383

Stefano A. Levi, Petra Me/a, Frank C. J. M. van Veggel, David N. Reinhoudt, Albert van den Berg and Niek F. van Hulst SIMULTANEOUS HYDRODYNAMIC AND ELECTROKINETIC FLOW CONTROL .................................................................................................................. 386

Ring-Ling Chien, Andrea Chow and J. Wallace Parce A MICRO-FLUIDIC PLATFORM FOR CONTACTLESS, DROPLETLESS DISPENSING OF HIGH-DENSITY PROTEIN MICRO-ARRAYS ......................... 389

P. -F. Indermuhle, P. Lin, M. X. Tan, F. G. Zaugg, P. Zwahlen and P. Wagner Plastic Machining PDMS MICROSTRUCTURES INTEGRATED WITH DETECTION ELEMENTS ................................................................................................................ 392

Michal Chudy, Piotr Prokaryn, Artur Dybko, Wojciech Wr6blewski and Zbigniew Brz6zka DEVELOPMENT OF PLASTIC MICRONEEDLES FOR TRANSDERMAL INTERFACING USING INJECTION MOLDING TECHNIQUES .......................... 395

Ramachandran Trichur, Sanghyo Kim, Xiaoshan Zhu, Ji Won Suk, Chien-Chong Hong, Jin- Woo Choi and Chong H. Ahn A NOVEL BONDING TECHNIQUE FOR MICRO POLYMER CHIP USING SACRIFICIAL CHANNEL AND ADHESIVE PRINTING ...................................... 398

Masahiro Sugimoto, Hitoshi Saika, Shinsuke Shibata, Shouji Shinohara and Osamu Tabata. ADHESIVE AND DEAD VOLUME FREE INTERFACING BETWEEN PDMS MICROFLUIDIC CHANNELS .................................................................................. 401

Ho Kim, Kyung Won Ro, Kwanseop Lim, Nokyoung Park, Mina Kim and Jong Hoon Hahn HIGH PRESSURE THERMAL BONDING FOR SEALING OF PLASTIC MICROSTRUCTURES .............................................................................................. 404

Frederick Rosenberger, Eric Jones, Cheng Lee and Don DeVoe INNOVATIVE LASER MACHINING AND SURFACE MODIFICATION FOR PLASTIC MICROFLUIDIC CHIP ............................................................................. 407

Ji- Yen Cheng, Kai-Hsiung Hsu, Cheng- Wey Wei and Tai-Horng Young

LIQUID PHASE 3-D CHANNEL NETWORKS ....................................................... 410

Glenny Mensing and David J. Beebe

xxi

DESIGN AND FABRICATION OF METAL ELECTRODES IMPLANTED PDMS STRUCTURES FOR MICRO FLOW DEVICE ........................................................ .413

M. Ishizuka, H. Hojo, M. Abe, K. Akahori, N. Honda, M. Mori, T. Sekiguti and S. Shoji STRONG BONDING OF METAL THIN-FILM MICROPATTERNS ON PDMS PLATE USING SELF-ASSEMBLED MONOLAYERS ............................................ 416

Yongmin Park, Young Chan Kim, Bong Chu Shim and long Hoon Hahn

Materials STUDY ON PROTEIN (IgG) ADSORPTION IN TERMS OF SURFACE MODIFICATION OF CYCLO OLEFIN COPOLYMER (COC) FOR PROTEIN BIOCHIP ..................................................................................................................... 419

lunhai Kai, Young-Soo Sohn and Chong H. Ahn 3-D MICROLFUIDIC NETWORKS FOR COMBINATORIAL CHEMISTRy ....... 422

Aniruddha Puntambekar, Ramachandran Trichur, lin Woo Choi and Chong H. Ahn EFFECT OF SURFACE MODIFICATION ON THERMOPLASTIC FUSION BONDING FOR 3-D MICROFLUIDICS .................................................................. 425

Aniruddha Puntambekar, Suresh Murugesan, Ramachandran Trichur, Hyoung l. Cho, Sanghyo Kim, lin-Woo Choi, Gregory Beaucage and Chong H. Ahn SURFACE MODIFICATION OF MICROGELS WITH N-ALKYL FATTY ACID LAYERS: ION GRADIENT PROPERTIES ............................................................... 428

Mary L. Kraft, David l. Beebe and leffrey S. Moore SURFACE MODIFICATION OF PDMS FOR CONTROL OF ELECTROOSMOTIC FLOW: CHARACTERIZATION USING ATOMIC AND CHEMICAL FORCE MICROSCOPY ........................................................................................................... 431

Bin Wang, Sarin Nita, J. Hugh Horton and Richard D. Oleschuk MOLECULARLY -IMPRINTED STATIONARY PHASES FOR PLASTIC MICROCHIP-BASED CAPILLARY ELECTRO CHROMATOGRAPHY ............... 434

Alyssa C. Henry, David J. Ross and Laurie E. Locascio CONTINUOUS SELF ARRANGEMENT OF NANOPARTICLES IN A MICRO-CAPILLARY ................................................................................................ 437

Hongzhi Wang, Xianying Li, Hiroyuki Nakamura, Masaya Miyazaki and Hideaki Meada SURFACE MODIFICATION OF MICROMOLDS BY FLUOROCARBON FILMS ......................................................................................................................... 440

Taegon Kim, Namkyun Kim, Seung-Mo Ha, lin-Goo Park, Yoomin Ahn, Eunkyu Lee, Younggyu Chai, Nahmgyoo Cho, laebum Choo and Seungyoung Hwang

xxii

FUNCTIONALIZED PARYLENE COATINGS FOR MICROFLUIDIC APPLICATIONS ......................................................................................................... 443

Jorg Lahann, Mercedes Balcells, Hang Lu, Teresa Rodon, Klavs F. Jensen and Robert Langer PHOTORESIST-FREE MICROPATTERNING OF POLYMER SURFACES USED IN MICROANALYTICAL DEVICES ....................................................................... 446

Bikas Vaidya, Steven A. Soper and Robin L. McCarley A NOVEL IN-DEVICE ENZYME IMMOBILIZATION METHOD FOR BIOMEMS, DEMONSTRATED FOR A CONTINUOUS GLUCOSE MONITOR .................................................................................................................. 449

Stefan Zimmermann, Doerte Fienbork, Mathew Wasilik and Dorian Liepmann DESIGNING SURFACE CHEMISTRIES FOR A HIGH DENSITY CHEMICAL MICROARRAY .......................................................................................................... 452

Sammy Datwani, Emilie Johnson, Sherri Biondi, Gloria Cheng, Jyotsna Iyer and Ravi Vijayendran MICRO PEPTIDE ARRAY SYNTHESIS ON A CHIP ............................................. 455

Dong-Sik Shin, Do-Hyun Kim, Ki-Hoon Jang, Woo-Jae Chung, Chan-Ku Kang, Kook-Nyung Lee, Yong-Kweon Kim and Yoon-Sik Lee THE INTEGRATION OF MICRO ARRAY AND MICROCHANNEL USING PLASTIC CHIP ........................................................................................................... 458

Ji-Yen Cheng, Cheng-Wey Wei, Kai-Hsiung Hsu and Tai-Horng Young DIAGNOSTIC ANTIGENS AND ANTIBODIES PATTERNED BY MICRO STAMPING SYSTEM ................................................................................................ 461

Mon-Juan Lee, Haimei Huang, Cheng-Kung Chou, Yi-Chin Tsai, Fan-Gang Tseng and Ching-Chang Chieng SU-8 STRUCTURES FOR INTEGRATED HIGH-SPEED SCREENING .............. .464

Bonnie L. Gray, Ventzeslav P. Iordanov, Pasqualina M. Sarro and Andre Bossche MULTIMODE OPTICAL WAVEGUIDE SPECTRSCOPY OF AQUEOUS GPLD SOL .................................................................................................................. 467

Z. -M. Qi, N. Matsuda, T. Yoshida, J. Santos, A. Takatsu and K. Kato PRISM-FREE BROADBAND COUPLING APPROACH FOR SPECTROELECTROCHEMICAL CHARACTERIZATION OF SURFACE-IMMOBILIZED MOLECULES .............................................................. 470

Jose H. Santos, Naoki Matsuda, Zhi-Mei Qi, Akiko Takatsu, Kenji Kato and Takamitsu Yoshida SMART, TEMPERATURE-RESPONSIVE SURFACES ........................................... 473

Alison F. Smith and Robin L. McCarley

XXlll

ELECTROKINETIC TRAPPING OF PROTEINS IN CAST-TO-SHAPE MATRICES ................................................................................................................. 476

Alexander Artau, Anup K. Singh and Timothy J. Shepodd LIPID MEMBRANE MICROARRAY WITH DISCRETE CHAMBERS ................ 479

Yuji Murakami, Ryotaro Hara, Takeshi Kinpara, Yasutaka Matsubara and Eiichi Tamiya PROTEIN PATTERNING WITH PROGRAMMABLE SURFACE CHEMISTRY CHIPS .......................................................................................................................... 482

Y. Wang, X. Cheng, Y. Hanein, A. Shastry, D. D. Denton, B. D. Ratner and K. F. Bohringer Electrochemical Detection MULTICHANNEL CAPILLARY ELECTROCHROMATOGRAPHY PMMA MICRODEVICE WITH INTEGRATED PULSED CONDUCTIVITY DETECTOR ................................................................................................................ 485

Michelle Galloway, Wieslaw Stryjewski, Sean Ford, Shawn D. Llopis, Bikas Vaidya and Steven A. Soper DEVELOPMENT OF ELECTROCHEMICAL METHODS FOR MONITORING OF SINGLE MACROMOLECULE PROCESSES .......................................................... 488

Masanori Ueda, Yuzuru Takamura, Yasuhiro Horiike and Yoshinobu Baba SEPARATION AND DETECTION OF ORGANIC ACIDS IN A CE MICRODEVICE WITH CONTACTLESS FOUR-ELECTRODE CONDUCTIVITY DETECTION ............................................................................... 491

F. Laugere, G. van der Steen, J. Bastemeijer, R. M. Guijt, P. M. Sarro, M. J. Vellekoop and A. Bossche BIOANALYTICAL DEVICE FOR HIGHLY SELECTIVE MEASUREMENT OF IN VIVO BIOCHEMICALS ............................................................................................ 494

Katsuyoshi Hayashi, Osamu Niwa, Tianyan You, Ryoji Kurita, Tsutomu Horiuchi, Shigeru Hirono and Kenji Sunagawa A NOVEL CONTACTLESS IMPEDANCE DETECTOR WITH SHALLOW INSULATION GAP .................................................................................................... 497

Songyi Kim, Yongwon Jeong, Haehyung Lee, Yongseok Han, Moon Koo Kang, Doo Soo Chung, Jun Keun Chang and Kukjin Chun

Mass Spectrometry MICROMACHINED POLYMERIC TIP AS AN ELECTROSPRAY IONIZATION SOURCE ..................................................................................................................... 500

Jun Kameoka, Reid Orth and Harld. G. Craighead MODIFIED POROUS SILICON SURFACES AS DIOS-MS SAMPLE PLATES ... 503

Santeri Tuomikoski, Katri Huikko, Pekka Ostman, Kestas Grigoras, Marc Baumann, Risto Kostiainen, Sami Franssila and Tapio Kotiaho

xxiv

PDMS ELECTROSPRAY DEVICES FABRICATED BY PDMS-DIAMOND-COATED SU-8 MASTERS ....................................................... 506

K. Huikko, P. Ostman, K. Grigoras, S. Tuomikoski, V. -M. Iiainen, A. Soininen, A. Manz, S. Franssila, R. Kostiainen and T. Kotiaho MICROFLUIDIC DEVICE WITH INTEGRATED PROTEIN DIGESTION, PEPTIDE SEPARATION AND NANOELECTROSPRAY INTERFACE ON POLY(DIMETHYLSILOXANE) PDMS SUBSTRATE ............................................ 509

Wang-Chou Sung, Pao-Chi Liao, Pang-Wei Chen, Gwo-Bin Lee, Mong-Kuan Chou and Shu-Hui Chen Novel Detection Techniques QUANTITATIVE MEASUREMENT AND CONTROL OF ELECTROKINETICALLY DRIVEN FLOW IN MICROSPACE ............................. 512

Yohei Sato, Koichi Hishida and Masanobu Maeda CONTROLLING CELL DEVELOPMENT BY MICROFLUIDIC TECHNIQUES: A STEP TOWARDS WHOLE-CELL BIOSENSORS WITH DEFINED BIOLOGICAL FEATURES ................................................................................................................. 515

Fabrice Morin, Matthieu Denoual, Laurent Griscom, Bruno LePioufle, Hiroyuki Fujita and Eiichi Tamiya PROTEIN DYNAMICS STUDY USING A CONTINUOUS-FLOW MICROFLUIDIC MIXER .......................................................................................... 518

Masaya Kakuta, Peter Hismann, Bernhald Lendl, Dimuthu A. Jayawickrama, Andrew M. Wolters, Jonathan V. Sweedler and Andreas Manz Environmental Assays FIELD TESTING AND NEW APPLICATIONS OF A GAS PHASE MINIATURE CHEMICAL ANALYSIS SYSTEMS (IlCHEMLABTM) ........................................... 521

Curtis Mowry, Richard Kottenstette and Patrick Lewis

RESULTS FROM IlCHEMLABTM/CB, A PORTABLE SYSTEM FOR DETECTING CHEMICAL AND BIOLOGICAL WARFARE AGENTS ........................................ 524

Julia A. Fruetel, Brent A. Horn, Jay A. A. West, James F. Stamps, Victoria A. Vandernoot, Mary Clare Stoddard, Ronald F. Renzi and Debbie Padgen DEVELOPMENT OF MICRO DETECTOR FOR BENZO[A]PYRENE MONITORING ........................................................................................................... 527

Hiroyuki Masaki, Hironori Susaki, Kenji Uchiyama, Masayoshi Ito and Takashi Korenaga DEVELOPMENT AND EVALUATION OF A GAS ABSORPTION MICROCHIP USING POROUS GLASS PLATE ............................................................................. 530

Yasunari Ono, Hiromi Yamashita, Kenji Uchiyama and Takashi Korenaga

xxv

DEVELOPMENT AND OPTIMIZATION OF AIR-POLLUTANT MONITORING DEVICE INCORPORATING WITH MICROCHIP .................................................. 533

Yoshimasa Takabayashi, Kenji Uchiyama and Takashi Korenaga PORTABLE SYSTEM FOR SELECTIVE DETECTION OF AROMATIC VOC MIXTURE-GASES USING A MICROFLUIDIC DEVICE ...................................... 536

Tsutomu Horiuchi, Yuko Ueno, Osamu Niwa, Hao-shen Zhou, Takeo Yamada and Itaru Honma Separation Science FREE FLOW ELECTROPHORESIS DEVICE SHOWING SUB-SECOND ISOELECTRIC FOCUSING FOR A PEPTIDE ......................................................... 539

Yi Xu, Chao-xuan Zhang and Andreas Manz A NEW APPLICATION BY MICROFABRICATED FREE FLOW ELECTROPHORESIS MODULE .............................................................................. 542

Nobuyoshi Tajima, Jun Funazaki, Hirobumi Suzuki and Etsuo Shinohara NANO-FABRICATED SIZE EXCLUSION CHROMATOGRAPH .......................... 545

Danielle Svehla, Sabrina Feldman, Jason Feldman, Frank Grunthaner, Partha Shakkottai, Linda del Castillo and Victor White PINPOINT INJECTION AND DIELECTROPHORETIC FLOATING OF AUTONOMOUSLY ALIGNED MICROTOOLS ...................................................... 548

Fumihito Arai, Hisataka Maruyama, Akihiko Ichikawa, Toshihiro Sakami and Toshio Fukuda RAPID PRECOLUMN REACTION AND CHIRAL SEPARATION ON AN INTEGRATED MICROCHIP ..................................................................................... 551

Kyung Won Ro, Ho Kim and Jong Hoon Hahn STEPWISE GRADIENT OF LINEAR POLYMER MATRICES IN MICROCHIP ELECTROPHORESIS FOR HIGH RESOLUTION SEPARATION OF DNA ......... 554

Lihua Zhang, Fuquan Dang, Feng Xu and Yoshinobu Baba CONTINUOUS CELL PREPARATION USING TRAVELING WAVE DIELECTROPHORESIS ............................................................................................ 557

Yongmin Park, Ji Mee Kim, Nokyoung Park and Jang Hoon Hahn A NEW PLASTIC CE CHIP WITH WIDE OPTICAL CLARITY USING CYCLIC OLEFIN COPOLYMERS(COC) ................................................................................ 560

Ramachandran Trichur, Sanghyo Kim, Se Hwan Lee, Yasser A. Abdelaziez, Dustin E. Starkey, H. Brian Halsall, William R. Heineman and Chong H. Ahn MICROCHIP ELETROPHORESIS OF OLIGOSACCHARIDES FROM GLYCOPROTEIN ....................................................................................................... 563

Fuquan Dang, Lihua Zhang and Yoshinobu Baba

xxvi

DIAGNOSIS OF HEPATITIS C VIRUS (HCV) THROUGH AFFINITY CHROMATOGRAPHY USING PHOTOLABILE MAGNETIC BEADS LINKED RNA APTAMER ON A CHIP .................................................................................... 566

SUhyung Cho, Sang-Ho Lee, Woo-Jae Chung, Yong-Kweon Kim, Yoon-Sik Lee and Byung-Gee Kim CONTINUOUS CELL PARTITIONING IN TWO-PHASE FLOW SYSTEM ......... 569

Jun'ichi Edahiro, Megumi Nakashima, Vivi Kasim, Masumi Yamada and Minoru Seki GENE MUTATION ASSAY BY AFFINITY MICROCHIP ELECTROPHORESIS USING DNA-POLYACRYLAMIDE CONJUGATE .................................................. 572

Tohru Takarada, Yuzo Hamaguchi, Masako Ogawa and Mizuo Maeda REMOVAL OF SODIUM ION AND CHIRAL ANALYSIS USING CROWN ETHER AS A CHIRAL SELECTOR IN MICROCHIP ELECTROPHORESIS ....... 575

Seung Il Cho, Jiyeon Shim, Min-Su Kim, Doo Soo Chung and Yong-Kweon Kim A MULTILAYER MICROELECTRODE ARRAY FOR PARTICLE SEPARATION BY DIELECTROPHORESIS ..................................................................................... 578

M. Frenea, N. Melaine, B. Le Pioufle, A. TlXier and H. Fujita CONTINUOUS FREE-FLOW ELECTROPHORESIS SEPARATION OF PROTEINS BY MICROFABRICATED CHAMBER WITH MICROMODULE FRACTION SEPARATOR .......................................................................................... 581

Hidesaburo Kobayashi, Katuyoshi Shimamura, Tomohiko Akaida, Kenji Sakano, Nobuyoshi Tajima, Jun Funazaki, Hirobumi Suzuki and Etsuo Shinohara VIRAL SEPARATIONS USING A MICROFABRICATED ELECTRICAL SPLITT SySTEM ..................................................................................................................... 584

Avinash D. Saldanha and Bruce K. Gale CHIP BASED SINGLE-COLUMN TITP (TRANSIENT ISOTACHOPHORESIS) SEPARATOR ............................................................................................................... 587

Yongseok Han, Yongwon Jeong, Songyi Kim, Haehyung Lee, Seok Chung, Doo Soo Chung, Jun Keun Chang, Moon Koo Kang and Kukjin Chun CAPILLARY ELECTROCHROMATOGRAPHY AND PRECONCENTRATION OF NEUTRAL COMPOUNDS ON PDMS MICROCHIPS ............................................ 590

Kyung Won Ro, Woo-Jin Chang, Ho Kim, Yoon-Mo Koo and Jong Hoon Hahn A MICROCONCENTRATOR FOR SENSORS AND CHROMATOGRAPHy ....... 593

Minhee Kim and Somenath Mitra MINIATURIZATION OF PROTEIN SEPARATION: ISOELECTRIC FOCUSING AND SDS-PAGE ......................................................................................................... 596

Jongyoon Han and Anup K. Singh

xxvii

ON-LINE MID-IR (QUANTUM CASCADE LASER AND FfIR SPECTROMETRIC) DETECTION IN CAPILLARY BASED SEPARATION SYSTEMS ................................................................................................................... 599

B. Lendl, M. Kolhed, P. Hinsmann, M. Haberkorn, P. Svasek and B. Karlberg MONOLITHIC SILICA COLUMNS FOR CAPILLARY HPLC .............................. 602

Norio Ishizuka, Hiroyoshi Minakuchi, Kazuki Nakanishi, Masanori Motokawa, Kouji Takahashi, Masahiro Furuno and Nobuo Tanaka

VOLUME 2 Day 3 Plenary 3 BEADS IN BIOCHEMICAL MICROFULUIDICS ................................................... 605

Patrick Griss, Helene Andersson, Wouter van der Wzjngaart and Goran Stemme Proteomics HIGH PERFORMANCE TWO DIMENSIONAL SEPARATIONS OF TRYPTIC DIGESTS ON MICROFLUIDIC DEVICES .............................................................. 608

Stephen C. Jacobson, Jeremy D. Ramsey, Christopher T. Culbertson and J. Michael Ramsey 2-D PROTEIN SEPARATION WITH CHROMATOGRAPHY AND ISOELECTRIC FOCUSING ................................................................................................................. 611

Masumi Yamada and Minoru Seki A 15 SECONDS PROTEIN SEPARATION BY EMPLOYING HIDRODYNAMIC FORCE ON A MICROCHIP ...................................................................................... 614 Mari Tabuchi, Yasuhiro Kuramitsu, Kazuyuki Nakamura and Yoshinobu Baba Microfluidics II INTEGRATED TEMPERATURE CONTROL SYSTEM FOR MICROFLUIDIC DEVICES .................................................................................................................... 617

A. Dodge, R. M. Guijt, G. W. K. van Dedem, N. F. de Rooij and E. Verpoorte PATTERNING FLOWS USING GROOVED SURFACES: APPLICATION TO MICROFLUIDICS ...................................................................................................... 620

Armand Ajdari, Abraham D. Stroock, Stephan K. Dertinger and George M. Whitesides MICRO-EVAPORATOR FOR PRETREATMENT OF ENVIRONMENTAL MONITORING ........................................................................................................... 623

Akira Koide, Tadashi Sano, Takeshi Harada and Ryou Miyake

xxviii

Sample Preparation AUTOLOGOUS BLOOD RECOVERY AND WASH IN MICROFLUIDIC CHANNEL ARRAYS UTILIZING ULTRASONIC STANDING WAVES ............... 625

Andreas Nilsson, Filip Petersson, Hans W. Persson, Henrik Jonsson and Thomas Laurell PLANAR ULTRA-FILTRATION CHIP FOR RAPID PLASMA SEPARATION BY DIFFUSION ................................................................................................................ 627

Kazuhiro Iida, Hisao Kawaura, Noriyuki Iguchi, Tohru Sano and Masakazu Baba MICROFLUIDIC DEVICE FOR BIOANALYSIS USING FREELY MOVING BEADS TRAPPED IN A RECIRCULATING FLOW ............................................... 630

G. -L. Lettieri, N. F. de Rooij and E. Verpoorte Detection Systems SPR IMAGING MEASUREMENTS OF PEPTIDE MICROARRAYS IN PDMS MICROFLUIDIC CHANNELS ON GOLD THIN FILMS ........................................ 633

Greta Wegner, Hye Jin Lee and Robert M. Corn SHAH CONVOLUTION FOURIER TRANSFORM DETECTION OF PARTICLE VELOCITIES BY USING AN INTEGRATED Ixl28 PLANAR WAVEGUIDE BEAMSPLITTER ....................................................................................................... 636

Klaus B. Mogensen, Yien C. Kwok, Jan C. T. Eijkel, Nickolaj J. Petersen, Andreas Manz and Jorg P. Kutter A MICRO VISCOSITY DETECTOR FOR USE IN MINIATURIZED CHEMICAL SEPARATION SYSTEMS .......................................................................................... 639

M. T. Blom, E. Chmela, F. H. J. van der Heyden, R. E. Oosterbroek, R. TIjssen, M. Elwenspoek and A. van den Berg

Plenary 4 TOWARDS INTEGRATED MICROSYSTEMS FOR CHEMICAL SYNTHESIS ... 642

Klavs F. Jensen Cell Manipulation in Flows ENABLING TECHNOLOGIES FOR A PERSONAL FLOW CYTOMETER (PARTD ...................................................................................................................... 646

C. Cabuz, A. Padmanabhan, B. Fritz, E. Cabuz, J. Schwichtenberg, P. Reutiman, B. Demers, T. Rezachek, E. Satren and F. Battrell LEUKOCYTES DISCRIMINATION BY IMPEDANCE SPECTROSCOPY FLOW CYTOMETRY ............................................................................................................ 649

Shady Gawad, Pascal Batard, Urban Seger, Stefan Metz and Philippe Renaud DESIGN AND FABRICATION OF MICROINANO-FLUIDIC CHIP PERFORMING SINGLE-CELL POSITIONING AND NANOLITER DRUG INJECTION FOR SINGLE-CELL ANALYSIS ......................................................... 652

Kwang-Seok Yun, Sang-II Lee, Gyun Min Lee and Euisik Yoon

xxix

Separation, Concepts A CIRCULAR AC ELECTROOSMOTIC MICROPUMP FOR CHROMATOGRAPHIC APPLICATIONS ................................................................ 655

S. Debesset, C. J. Hayden, C. Dalton, J. C. T. Eijkel and A. Manz PULSED FIELD ELECTROPHORESIS; A NEW APPROACH FOR HIGH THROUGHPUT EXPERIMENTATION FOR NON-FLUOROGENIC ASSAYS .... 658

Andrea Chow, Anubhav Tripathi, Yue Suo, Roger Dettloff, John Owicki and Wally Parce TEMPERATURE GRADIENT FOCUSING .............................................................. 661

David Ross and Laurie E. Loascio

Day 3 Posters Microfluidics II DEVELOPMENT OF ELECTROPHORETIC ANALYSIS SYSTEM USING MULTIPLE CHANNEL MICROCHIPS .................................................................... 664

Hisashi Hagiwara, Yoshinori Mishina, Mika Inoue, Masumi Yamada, Toshiyuki Sakurai, Teruo Fujii, Minoru Seki and Yoshiyuki Sakaki IMPROVED SAMPLE INJECTION METHOD ADAPTING HYDROPHOBIC PASSIVE VALVE SYSTEMS FORMICROFLUIDIC DEVICES ............................ 667

Nae Yoon Lee, Masumi Yamada and Minoru Seki A CLOSED-LOOP CALIBRATION SYSTEM FOR A MICRODIALYSIS-BASED LAB-ON-A-CHIP ....................................................................................................... 670

A. J. Sprenkels, D. G. Pijanowska, J. G. Bomer, S. Herber, K. M. van Delft, W. Olthuis and P. Bergveld FACTORS INFLUENCING RESPONSE TIME OF DEP MICROFLUIDIC ACTUATION .............................................................................................................. 673

T. B. Jones, T. [to, H. Yoo and R. Ahmed DROPLET FLIGHT STABILITY AND SAMPLE ENRICHMENT BY GAS-FLOW GUIDED DISPENSING ............................................................................................. 676

Simon Ekstrom, Jonas Bergkvist, Thomas Laurell and Johan Nilsson PROTEIN MICROARRAY PATTERNED BY A SURFACE-TENSION-DRIVEN STAMPING SYSTEM WITH DISCRETE DISPENSING CHANNELS .................. 679

Shih-Chang Lin, Yi-Chin. Tsai, Fan-Gang Tseng, Haimei Huang and Ching-Chang Chieng MICROMACHINED HOLLOW NEEDLE WITH INTEGRATED PRESSURE SENSOR FOR PRECISE, CALIBRATED INJECTION INTO CELLS AND EMBRYOS .................................................................................................................. 682

S. Zappe, X. J. Zhang, l. W. Jung, R. W. Bernstein, E. E. M. Furlong, M. Fish, M. Scott and o. Solgaard

xxx

FALLING-DROP INTERFACE FOR CONTINUOUS SAMPLE INTRODUCTION IN MICROFLUIDIC CHIPS ....................................................................................... 685

Qun Fang, Zhi-Jian Jia and Zhao-Lun Fang NEW ASSAYS AND BASED ON LAMINAR FLUID DIFFUSION INTERFACES -RESULTS FROM PROTOTYPE AND PRODUCT TESTING ................................. 688

Christopher J. Morris, Troy Daiber, Bernhard H. Weigl, Natasa Kesler and Ron L. Bardell A NEW APPROACH FOR FABRICATING A ZERO DEAD VOLUME ELECTROSPRAY TIP FOR NON-AQUEOUS MICROCHIP CE-MS .................... 691

Daria Petersen, Sami Varjo, Oliver Geschke, Marja-Liisa Riekkola and Jorg P. Kutter SILICONE RUBBER COUPLING FOR MICRO FLUIDIC DEVICES - THEORETICAL ANALYSIS OF SEALING PROPERTIES FOR INTERCONNECTION - ............................................................................................ 694

Tadahiro Hasegawa and Koji [kuta A RAPID, FLOW-THROUGH, DNA EXTRACTION MODULE FOR INTEGRATION INTO MICROFLUIDIC SYSTEMS ............................................... 697

Phillip Belgrader, Kevin Ness, Farzad Pourahmadi, Kevin Brounstein, M. Allen Northrup and Gary Long THE INFLUENCE OF FLOW CHANNEL GEOMETRY ON CAPTURE EFFICIENCY OF RARE CELLS USING PROTEIN A-ANTI HUMAN CD3 MAGNETIC BEADS .................................................................................................. 700

Vasile l. Furdui and D. Jed Harrison

ENGINEERING IMPROVEMENT OF THE GENERATION-2 ~CHEMLABTM BIOTOXIN DETECTOR ............................................................................................ 703

Ronald F. Renzi, James F. Stamps, Robert W Crocker, Boyd J. Wiedenman, Scott M. Ferko, Brent A. Horn, Dan Yee, Victoria A. Vandernoot, Jason A. West, Julia A. Fruetel, Yolanda Fintschenko and Mary Clare Stoddard SPATIALLY LOCALIZED VOLTAGE CONTROL IN GLASS MICROCHANNELS FOR ENHANCED SAMPLE HANDLING FLEXIBILITy ...................................... 706

R. M. Guijt, G. W K. van Dedem, N. F. de Rooij and E. Verpoorte Microfluidic Components INTEGRATED ELECTROSPRAY CHIP FOR MASS SPECTROMETRy ............. 709

Jun Xie, Qing He, Yu-Chong Tai, Jun Liu and Terry Lee DISSOLVABLE AND ASYMMETRIC HYDROGELS AS COMPONENTS FOR MICROFLUIDIC SYSTEMS ..................................................................................... 712

Qing Yu, Jeffrey S. Moore and David J. Beebe

xxxi

THE DEVELOPMENT OF QUITE LOW COST DISPOSABLE, RAPID, INDIVIDUALLY CONTROLLED MATRIX (10 x 10) MICROREACTOR SySTEM ..................................................................................................................... 715

Masahiro Kuwata, Hajime Sudo, Koichiro Kawano and Kaname Miyazaki

DEMONSTRATION OF HYDROGEL VOLUME CONTROL USING PULSE WIDTH MODULATION ............................................................................................ 718

Michael J. Bassetti and David J. Beebe

DEP-DRIVEN SIMULTANEOUS 2 x 2 DROPLET MIXER ARRAy ..................... 721

Masahide Gunji, T. B. Jones and Masao Washizu INTEGRATED THIN FILM TEMPERATURE SENSORS FOR POLYCARBONATE MICROFLUIDICS ................................................................... 724

Chris Kimball and Don L. De Voe

POLYIMIDE-BASED MICROFLUIDIC DEVICES WITH NANOPOROUS MEMBRANES FOR FILTRATION AND SEPARATION OF PARTICLES AND MOLECULES ............................................................................................................. 727

S. Metz, S. Gawad, Ch. Trautmann, A. Bertsch and Ph. Renaud MICRO ELECTROPHORESIS CHIPS WITH ON-CHIP OPTICAL WAVEGUIDES UTILIZING NOVEL BURIED SU-8/S0G DOUBLE LAyERS ............................... 730

Che-Hsin Lin, Gwo-Bin Lee, Chun-Che Lin and Shu-Hui Chen

DYNAMICS OF BUBBLES IN MICROCHANNELS .............................................. 133

Mads Jakob Jensen, Goran Goranovic and Henrik Bruus EXPERIMENMTAL VERIFICATION OF A BI-DIRECTIONAL DRIVING SYSTEM FOR MICROFLUIDS ................................................................................ 736

C. P. Jen, W. D. Wu, Y. C. Lin, G. G. Wu, C. C. Chang and Y. C. Chung MICROFABRICATION OF A SI MESH STRUCTURE DEPTH FILTER ............... 739

Yu Chen, Yubo Miao, Victor Samper, Fatimah Bte Mustafa, Qingxin Zhang, Chewkiat Heng, Huijen Lye and Tztmeng Lim

IN-SITU FABRICATION OF DIALYSIS MEMBRANES IN GLASS MICROCHANNELS USING LASER-INDUCED PHASE-SEPARATION POLyMERIZATION ................................................................................................... 742

Brian J. Kirby and Anup K. Singh

MICRO ULTRASONIC HOMOGENIZER CHIP MADE BY HYBRID MICROSTEREOLITHOGRAPHY ............................................................................ 745

Koji Ikuta, Yasuhiko Sasaki, Hiroyuki Maegawa, Shoji Maruo and Tadashiro Hasegawa

THE 1C-INJECTOR: A NEW ELECTROKINETIC DISPENSING ELEMENT FOR MICRODEVICE SEPARATIONS .............................................................................. 748

Charles A. Emrich and Richard A. Mathies

xxxii

MANIPULATION OF SUSPENDED PARTICLES IN A LAMINAR FLOW .......... 751

Andreas Nilsson, Filip Peterson, Hans W. Persson, Henrik Jonsson and Thomas Laurell POLYMER-BASED ACTUATORS INTEGRATED INTO MICROFLUIDIC SYSTEMS ................................................................................................................... 754

Nikolas Chronis and Luke P. Lee

BARRIER EMBEDDED CHAOTIC MICROMIXER. .............................................. 757

Dong Sung Kim, Seok Woo Lee, Tai Hun Kwon and Seung S. Lee

MAGNETICALLY ACTUATED COLLOIDAL CHAINS IN MICROCHANNELS ................................................................................................... 760

Sibani Lisa Biswal and Alice P. Gast

Nanotechnology SIZE EXCLUSION CHROMATOGRAPHY WITH PATTERNED NANO-PILLAR ARRAY ....................................................................................................................... 763

Masakazu Baba, Tohru Sano, Noriyuki Iguchi, Kazuhiro !ida, Toshitsugu Sakamoto and Hisao Kawaura SIZE EXCLUSION CHROMATOGRAPHY USING SELF-ORGANIZED NANO-PORES IN ANODIC POROUS ALUMINA ................................................. 766

Tohru Sano, Noriyuki Iguchi, Kazuhiro !ida, Hisao Kawaura and Toshitsugu Sakamoto NANOCHANNEL ON FUSED-SILICA MICROCHIP AND LIQUID PROPERTIES INVESTIGATION BY TIME-RESOLVED FLUORESCENCE MEASUREMENTS .................................................................................................... 769

Akihide Hibara, Takumi Saito, Haeng-Boo Kim, Manabu Tokeshi, Takeshi Ooi, Masayuki Nakao and Takehiko Kitamori

CONTROLLED QUANTUM DOT SYNTHESIS WITHIN MICROFLUIDIC CIRCUITS ................................................................................................................... 772

Joshua B. Edel, Robin Fortt, John C. de Mello and Andrew J. de Mello PDMS NANOSTRUCTURES FOR BIOMOLECULES ANALYSIS ....................... 775

Eric Leclerc, Takatoki Yamamoto and Teruo Fujii

NANOSTRUCTURED ELECTRODES FOR NEURAL CHIP APPLICATIONS .... 778

Karen Cheung, Yang-Kyu Choi, TIm Kubow and Luke P. Lee MOLECULAR FILTER-NANOSIEVE ...................................................................... 781

Terrell D. Neal and Axel Scherer

Cellular Analysis

DEVELOPMENT OF MICROFLUIDIC SHEAR ASSAYS FOR QUANTITATIVE ANALYSIS OF CELL ADHESION ........................................................................... 784

Hang Lu, Lily Y. Koo, Linda Griffith and Klavs F. Jensen

xxxiii

A MICROFLUIDIC BIOSYSTEM FOR METABOLIC MONITORING OF HUMAN ISLET CELLS WITH INTEGRATED BIOSENSORS .............................. 787

Chuan Gao, Horacio L. Rodriguez Rilo, Jin- Woo Choi and Chong H. Ahn A LABCHIP@ CELL SORTING AND DEPOSITION SYSTEM BASED ON A RAPID FUNCTIONAL RESPONSE TO STIMULATION OF PURINOCEPTORS ..................................................................................................... 790

Jeffrey Wolk, Anhubav Tripathi, Roy Sandberg, Yue Suo, Andrea Chow and Gary Wada POSITIONING AND IMMOBILIZATION OF DROSOPHILA EMBRYOS IN 2-D ARRAYS FOR DRUG INJECTION ........................................................................... 793

Ralph W. Bernstein, Xiaojing Zhang, Stefan Zappe, Matt Fish, Matthew Scott and Olav Solgaard TOWARDS SINGLE-CELL-CONTROLLED ELECTROPORATION IN A MICROFLUIDIC DEVICE ........................................................................................ 796

Urban Seger, Shady Gawad, Alexander Scheer and Philippe Renaud ENHANCED VELOCITY GRADIENTS WITHIN MICROFLUIDICS FOR CELLULAR MANIPULATION ................................................................................. 799

Dino Di Carlo and Luke P. Lee CELL ANALYSIS IN VALVED MICROFLUIDIC DEVICES ................................. 802

Aaron R. Wheeler, Andrew M. Leach and Richard N. Zare A MULTI-PURPOSE MICRO SYSTEM FOR ELECTROPHYSIOLOGICAL ANALYSES OF SINGLE CELLS .............................................................................. 805

Arum Han, Eileen Moss, Richard D. Rabbitt and A. Bruno Frazier TOPOLOGICALLY INDUCED BACTERIAL AGGREGATION IN A MICROFABRICATED RANDOM MAZE ................................................................ 808

Sungsu Park, Emil Yuzbashyan, Peter Wolanin, Jeffy Stock, Pascal Silberzan and Robert Austin MONITORING OF HUMAN CHEMILUMINESCENT REPORTER CELL LINES EXPRESSING G-PROTEIN-COUPLED RECEPTORS IN A MICROFLUIDIC SYSTEM ..................................................................................................................... 811

Richard Davidsson, Jesper Bristulf, Knut Kotarsky, BjOrn aIde, Christer Owman, Martin Bengtsson, Thomas Laurell and Jenny Emneus

DEVELOPMENT OF NOVEL BIOASSAY SYSTEM USING MICROPATTERNED MAMMALIAN CELLS .............................................................................................. 814

Hirokazu Kaji, Matsuhiko Nishizawa and Tomokazu Matsue DNA EXTRACTION FROM BACTERIAL CELLS BY REVERSE ELECTROPORATION AND SPLITT METHODS ON A MICROFABRICATED DEVICE ...................................................................................................................... 817

Melanie Fennah and Andreas Manz

xxxiv

CELL SEPARATION WITH TRANSIENT ADHESIONS ........................................ 820

Wesley C. Chang, Luke P. Lee and Dorian Liepmann GENE MANIPULATION SYSTEM ON INTEGRATED POLYMER MICROCHIPS ............................................................................................................. 823

Morio Fukui, Masumi Yamada, Takaaki Saeki, Yasuhiro Kakigi, Nae Yoon Lee and Minoru Seki THREE DIMENSIONAL PDMS MICROSTRUCTURE FOR 3T3-Ll FIBROBLAST AND HEP G2 CELLS PERFUSION CULTURE ............................. 826

Eric Leclerc, Yasuyuki Sakai and Teruo Fujii DIELECTROPHORETIC CHROMATOGRAPHY OF CELLS ................................ 829

David Holmes and Hywel Morgan CELLULAR ANALYSIS SYSTEM OF HISTAMINE RELEASE ON A MICROCHIP ............................................................................................................... 832

Takahito Tokuyama, Shin-ichiro Fujii, Mitsuru Abo and Akira Okubo SYNCHRONIZATION OF BACTERIAL CULTURES IN A MICROSYSTEM ..... 835

T. Fettah Kosar, Deirdre R. Meldrum and Mark R. Holl SINGLE CELL ANALYSIS OF BOVINE CHROMAFFIN CELLS USING MICRO ELECTRICAL IMPEDANCE SPECTROSCOPY ..................................................... 838

Swomitra K. Mohanty, Surendra K. Ravula, Katherin Engisch and A. Bruno Frazier ON-CHIP NEURAL CELL CULTIVATION SYSTEM FOR LONG-TERM OBSERVATION WITH MULTI ELECTRODE AND MICRO-CHAMBER ARRAYS .................................................................................. 841

Kazunori Takahashi, Yoshihiro Sugio, Hiroyuki Moriguchi, Yasuhiko limbo and Kenji Yasuda SELF-ASSEMBLED MAGNETIC BEADS FOR CELL SORTING ........................ 844

Cecile Goubault, lean-Louis Viovy and Jerome Bibette OBSERVATION OF EXTREMELY LOW TRANSMEMBRANE POTENTIAL OF CELLS IN ELECTROPORATION USING MICROCHIPS ...................................... 847

Yu-Cheng Lin, Ming-Yuan Huang and Min Li Drug Discovery ON CHIP GENERATION AND REACTION OF UNSTABLE INTERMEDIATES: MONOLITHIC MICROREACTORS FOR DIAZONIUM CHEMISTRIES ............. 850

Robin Fortt, Robert C. R. Wootton and Andrew l. de Mello MICROSYSTEMS TECHNOLOGY FOR CELL SCREENING IN NEW MEDICINES DISCOVERY ........................................................................................ 853

Norbert Klauke, Godfrey Smith and Jon Cooper

xxxv

ELECTROPHYSIOLOGICAL HIGH THROUGHPUT DRUG SCREENING SySTEM .................................. ; .................................................................................. 856

Nobuhiko Ozaki, Ryuta Ogawa, Hirokazu Sugihara, Fumiaki Emoto, Makoto Tanabe, Satoshi Konishi and Hiroaki Oka A COMBINATORIAL LIBRARY OF PYRAZOLES PERFORMED IN AN AUTOMATED MICRO REACTOR SySTEM .......................................................... 859

Eduardo Garcia-Egido, Valerie Spikmans, Stephanie Y. F. Wong and Brian H. Warrington HIGH-THROUGHPUT SCREENING OF ANTICANCER DRUGS USING MICRO ARRAY BASED CELL CHIP ....................................................................... 862

Sathuluri Ramachandra Rao, Yoshinori Akagi, Yasutaka Morita and Eiichi Tamiya Biochemical Applications ELECTRIC FIELD ASSISTED EXTRACTION AND FOCUSING OF FINGERPRINT RESIDUES BY MEANS OF A MICROFLUIDIC DEVICE .......... 865

Silvia Valussi and Andreas Manz DESIGN OF A MICROFLUIDIC FLOW INJECTION ANALYSIS SySTEM ........ 868

Andrew M. Leach, Aaron R. Wheeler and Richard N. Zare BIOCHEMICAL REACTION ON A CHIP USING A QUANTITATIVE VOLUME CONTROL .................................................................................................................. 871

Sang-Ho Lee, Chang-Soo Lee, Youn-Suk Choi, Kwang-Won Suh, Byung-Gee Kim and Yong-Kweon Kim INTEGRATED DEVICE FOR POINT-OF-CARE GENOTYPING .......................... 874

Eugen Ermantraut, Torsten Schulz and Thomas Ellinger PHOTOCYANATION OF AROMATIC HYDROCARBONS ACROSS OIUWATER INTERFACE IN POLYMER MICROFLUIDIC CHIPS ............................................ 876

Fumihiko Kitagawa, Kosei Veno and Noboru Kitamura MICROFLUIDIC PROTEIN PATTERNING CONTROLLED BY HYDROPHOBIC SURFACE AND PNEUMATIC CONTROL ................................ 879

Chang-Soo Lee, Sang-Ho Lee, Yun-Gon Kim, Yong-Kweon Kim and Byung-Gee Kim FABRICATION AND APPLICATIONS OF POLYMER-BASED MICROCHANNEL-HEATER CHIP AS MICROREACTOR ................................... 882

Yukiko Hosoda, Kosei Veno, Shoji Ishizaka and Noboru Kitamura CdSe NANOPARTICLES FORMATION IN A MICROS PACE AND THEIR PROPERTIES .............................................................................................................. 885

Hiroyuki Nakamura, Masaya Miyazaki, Hideaki Maeda, Asuka Tashiro, Kazuya Ogino and Takanori Watari TO PLACE CELLS AS AN ARRAY USING ASPIRATION TECHNIQUE ............ 888

Agnes Tuier-Mita, Yoshio Mita, Katell Cozic, Marie Frenea, Bruno Le Pioufle and Hiroyuki Fujita

xxxvi

Novel Quantification Strategies AN INTEGRATED CHEMILUMINESCENCE DETECTOR FOR MEASURING ENZYMATICALLY GENERATED HYDROGENPEROXIDE ................................ 891

Anders M. Jorgensen, Daria Petersen and Oliver Geschke AN ATMOSPHERIC-PRESSURE MICROPLASMA JET SOURCE FOR OPTICAL EMISSION SPECTROSCOPY OF LIQUID SAMPLE ............................................. 894

T. Koidesawa, T. Ichiki and Y. Horiike RAPID DETECTION OF MICROORGANISMS USING MICROWAVES ............. 897

Grant Hollis, Daniel B. Oerther and Ian Papautsky QUANTIFICATION OF MOLECULAR MIXING WITH EPIFLUORESCENCE IMAGING ................................................................................................................... 900

Sneha Madhavan-Reese, Daniel Lim, Jyotirmoy Mazumder and E. F. Hasselbrink, JT. EVALUATION OF THE CAPTURE EFFICIENCY FOR DNA IN A FLOW THROUGH DEVICE .................................................................................................. 903

Olena Melnychuk and D. Jed Harrison PRACTICAL STUDIES ON MULTI-CHANNEL OPERATION OF A NOVEL HIGHLY SENSITIVE QUARTZ CRYSTAL MICROBALANCE-BASED PLANAR BIOSENSOR ............................................................................................................... 906

Hiroyuki Sota, Hiroshi Yoshimine, Yasuro Shinohara, Lars Fiigerstam, Patrie Fricking, Mattias Vangbo and Yoshio Okahata. SENSITIVITY ENHANCEMENT IN CHIP-BASED CAPILLARY ELECTROPHORESIS SYSTEMS BY A HADAMARD TRANSFORM APPROACH ................................................................................................................ 909

Tao Zhang, Qun Fang and Zhao-Lun Fang Day 4 Plenary 5 MICROINANO MACHINED DEVICES PROBING INTO NANO/BIO WORLD ...................................................................................................................... 912

Hiroyuki Fujita BIO-CHEMICAL ANALYSIS ON MICROFABRICATED POLYMER CHIPS .......................................................................................................................... 918

Koji Shiomoide, Kazuma Mawatari, Shigemi Mukaiyama and Hiroyuki Fukui Single Cell Analysis BIOCHEMICAL SIGNAL TRANSFER USING LIPOSOMES IN MICROFLUIDIC CHANNELS ................................................................................................................ 922

1. A. Gimm, A. E. Ruoho and D. 1. Beebe

xxxvii

NOVEL BIOMOLECULE SORTER USING THERMOSENSITVE HYDROGEL IN MICRO FLOW SySTEM ........................................................................................... 925

Yoshitaka Shirasaki, Hiroshi Makazu, Koichi Tashiro, Shingo Ikeda, Tetsushi Sekiguchi, Shuichi Shoji, Shoichiro Tsukita and Takashi Funatsu FIXATION AND ISOLATION OF MICROORGANISMS BY LOCAL VISCOSITY CONTROL OF METHYL CELLULOSE SOLUTION ............................................. 928

Fumihito Arai, Akihiko Ichikawa, Toshio Fukuda and Tohoru Katsuragi Nano-microfabrication CHEMICO-FUNCTIONAL MEMBRANE FOR INTEGRATED CHEMICAL PROCESSES ON A MICROCHIP ............................................................................. 931

Hideaki Hisamoto, Yuki Shimizu, Kenji Uchiyama, Manabu Tokeshi, Yoshikuni Kikutani, Akihide Hibara and Takehiko Kitamori IN SITU FABRICATED MICROCHANNELS USING POROUS POLYMER AND XENON DIFLUORIDE ETCHANT .......................................................................... 934

George C. Lopez, Alan J. Rosenbloom, Victor W. Weedn and Kaigham J. Gabriel DIRECT NANOMANIPULATION TOOLS FOR BIOLOGICAL SAMPLES ......... 937

Shoji Maruo, Koji Ikuta and Hayato Korogi DNA System MICROFLUIDIC CIRCUIT FOR INTEGRATED DNA SEQUENCING PRODUCT PURIFICATION AND ANALySIS ............................................................................ 940

Brian M. Paegel, Stephanie H. I. Yeung, James R. Scherer and Richard A. Mathies FULLY INTEGRATED MCM MICROFLUIDIC SYSTEM FOR DNA ANALySIS .................................................................................................................. 943

Robin H. Liu, Justin Bonanno, Jianing Yang, Dale Ganser, Ralf Lenigk, Dave Rhine, Thomas Smekal, Randy Stevens and Piotr Grodzinski AUTOMATED DNA FRACTION COLLECTION ON GLASS MICROCHIPS ...... 946 Abdel Minalla, Li Ding, Luc Bousse and Robert Dubrow Microfluidics III A DISPOSABLE ON-CHIP AIR DETONATOR FOR DRIVING FLUIDS ON POINT -OF-CARE SYSTEMS .................................................................................... 949

Chien-Chong Hong, Jin- Woo Choi and Chong H. Ahn MICROFLUIDIC CAPILLARY SYSTEMS FOR THE AUTONOMOUS TRANSPORT OF BIO/CHEMICALS ........................................................................ 952

D. Juncker, H. Schmid, U. Drechsler, H. Wolf, B. Michel, N. de Rooij and E. Delamarche. ELECTRIC DAMAGE FREE SEPARATION CHIP OF LYMPHOCYTE SUB-UNIT EMPLOYING HIGH PRESSURE ELECTROOSMOSIS PUMP ............................. 955

Hiroyuki Onoda, Yuzuru Takamura and Yasuhiro Horiike

xxxviii

Authors index ........................................................................................................ 959

Subject index ......................................................................................................... 972

xxxix