(Ft,LE Case Number: 20120406772

Sample ID 3 Description swab casings

Run 31-Jan-13 . . 0851179 nsd

021511 nsd

075820 nsd

C5F1PO nsd

0351358 nsd

TH01 nsd

0135317 nsd

0165539 nsd

0251338 nsd

0195433 nsd

vWA nsd

TPOX nsd

018551 nsd

AMEL nsd

055818 nsd

FGA nsd

Sample ID

Run

0851179 021511 075820 C5F1PO

0351358 TH01

0135317 0165539 0251338 0195433

vWA TPOX

018551 AMEL

055818 FGA

Florida Department of Law Enforcement STR SUMMARY SHEET

Approved 01 May 2012

Date: 02/11/13

-:-··

Date: ., �- \� Initials:

FDLE#: --FDLE Case #: �o \d-. oL-io l_p T --:=t �

D2te:J

Oitho-di'l.: 1 I . I a-napt

spot I tube I overiayf

Po sf

I$$Uet! 'from 8WN10 March 25, 20i0

P3n I HEMATRACE Date:/

K! Lot[ Ser.le!i Fcr.P30: . Ex!;. Da:e: . I

. .

Area Cleaned :

PHADEBAS.

. . 1:50j

O.SM ... . .. · -·

. ... -

1

2

3

4a

Florida Department of Law Enforcement

FD Biology Section ·.

SAMPLE PREPARATION WORKSHEET

Initials

..... ;;)

"7)

I

DNA IQ™ LYSIS BUFFER METHOD- NON-DIFFERENTIAL / Date: . C;i ... . Analyst: Area: / Cleaned: K

-Vol. Sample I D I Description Case # I Comments (IJL)

lsw20120406772_Ex3 1t lsw20120406772_RBQ lsw20 12040680 1_Ex1 lsw20120406801_Ex2 lsw20120406801_Ex5 lsw20120406801_Ex6 lsw20120406801_Ex7 lsw20120406801_Ex8 lsw20120406801_RBQ

Place the sample cutting into a sterile extraction tube.

Add 1001JL DNA IQ™ Lysis Buffer with DIT to the sample tube.

Vortex and b�iefly centrifuge to force the cutting into the buffer.

FTA Cards

Sample I D I Description Case # I Comments

lsw20120406045 Ex1 lsw20120406045 RBQ

lsw20110404779_Ex9.1 -- :·-1 ; '

lsw2011 0404779_Ex9.2 lsw2011 0404779_RBQ

J.

?.

-Vol. (�L)

DNA IQ™ Lysis Buffer with OTT Lot: ·;

Temperature:

Start Time:

/ Incubate in a dry heat block at 95°C for approximately 30 minutes. Stop Time:

4b ----;. 0 ( General Temperature: '10 -� .. Start Time: : Incubate in a dry heat block at 70°C for approximately 30 minutes to overnight.

Stop Time:

Allow the sample to reach room temperature and briefly centrifuge to remove any condensation from the cap.

6 Optional: Transfer the substrate into a spin basket. Centrifuge at maximum speed for 2 Remove the basket from the extract tube and retain the substrate . . ;!f\ ·,�0 � \

Optional: DNA Concentration I Wash

Date: Analyst: Area: Cleaned: Microcon Lot: DNA IQ™ Lysis Buffer with OTT Lot: I I

I· Comments:

Note: Samples can be left in the buffer for a maximum of 48 hours prior to the start of automated extraction by the BIOMEK® 3000. B Pipette a maximum of 100�L of the sample lysate into the

appropriate wells of a 96 deep-well plate. - . . .

Time I Date Prepared:

Time I Date Loaded�

, ·:-1 .:.:-s r .

� G\l

I

I :

BIO PreProcNonDiff2col (14)1 Issued from BEW1 o March 25, 201 o

---- · - -- -- ---·-- -- - - - -- -

CFDLE Sample ID Description

1 lsw:20120406772 Ex3

2 lsw20120406772_RBQ

3 lsw20120406801_Ex1

4 lsw20120406801_Ex2

5 lsw20120406801_Ex5

6 �w20120406801 Ex6

7 lsw20120406801_Ex7

8 lsw20120406801 Ex8

9 lsw20120406801 RBQ

0 lsw20120406045 Ex1 1 1

1 1

1 1 1

1 lsw20120406045 RBQ 2 lsw2011 0404779_Ex9. 1

3 lsw2011 0404779_Ex9.2

4 lsw20110404779 RBQ

5 lsw20090401384_Ex2.3

6 lsw20090401384_RBK-3

1 7 lsw20120406645_Ex6

1 8 lsw20120406645_RBK

9 lsw20120406801_Ex3 1 2

2

22

23

24

25

26

27

28

29

30

31

32

33

34 35

36

37

3,8

39

40

0 lsw20120406801_RBK-FTA

1 lsw20120406801 Ex4

lsw20120406801_RBK

. ·lsw20120406045 Ex2

lsw20120406045 RBK . .

·I�W20120406771_Ex1.1

lsw20120406771_RBK

lsw20 120404249 _ Ex5

lsw20 120404249 _RBK

lsw20120406578_Ex1

lsw20 120406578 _RBK

lsw20120406998 Ex1

lsw20120406998 RBK lsw20 120407000 _ Ex1

lsw20120407000 RBK

lsw20120406771_Ex1.2sp

lsw20120406771_Ex1.2epi

lsw20120406771_RBQsp

·. lsw20120406771_RBQepi

edmh_20120407111_1

edmh_20120407111_2

-. -- ----- -

Florida Department of Law Enforcement

Biology Section

Biomek® 3000 Extraction Set

Date: 30-Jan-13

B3K Plate Name:

Case #I Comments KIQ

Q

Q

Q

Q

Q

Q

Q

Q

Q

Q

Q

Q

Q

Q

K

K

K

K

K

K

K

K

K

K

K

K

K

K

K

K

K

K

K

K

Q

Q

Q

Q

Q

Sample ID Description

1 edmh 20120407111 3

2 edmh_20120407111 4

3 edmh_20120407111

4

4

4

4

4

4 4

4

4

5

5

5

5

5

5

5

5

5

5

6

6

6

6

64

65

66

RBQ

4 edmh_20120407113_1

5 edmh_20120407113_2

6 edmh_20120407113_RBQ

7 edmh_20120402713_4

8 edmh_20120402713_RBQ2

9 edmh_20130400029 1

0 edmh_20130400029_2

1 edmh 20130400029 3

2 edmh_20130400029_4

3 edmh_20130400029 RBQ

4 edmh_20120407111 5 FTA

5 edmh_20120407111_RBQ_FTA

6 edmh_20120407111 6

7 edmh_20120407111_RBK

8 edmh_20120407113_3

9 edmh_20120407113_ 4

0 edmh_20120407113_5

1 edmh 20120407113 RBK

2 lsw20130400493 Ex2

3 lsw20130400493 RBQ

empty1

jtm20120404427 2 mn2

jtm20120404427 _RBQ mn2

67

68

69 70

71

72

73

74 75

76 77 78

79

80

Case # I Comments KIQ

Q

Q

Q

Q

Q

Q Q

Q

Q

Q

Q

Q

Q K

K

K

K

K

K

K K Q

Q

plexor only Q plexor only Q

/\II II Dill./ n-:rto nrinin�k �rR m�int::�inP.d in the laboratory � p

�

CFDLE Florida Department of Law Enforcement Biology Section

BIOMEK® 3000 Sample Run Worksheet

for DNA IQ™ and Plexor® HY

DNA IQ™ Lot#:

End Column:

0000036314 8

Lysis Buffer w/ OTT lot#:

1X Wash Buffer lot#:

Exp Date: 23 Oct 2015 Elution Vol: 60 1

B-158 B-158

B3K Plate Name: AR30Jan13 Sample Plate was centrifuged prior to removal of sealing film & rf DNA IQ Date Run: 30-Jan-13 Time Run:

A

B

c

D

E

F O«J4 779 _ Ex6 RBQ

G Ex7 Ex2.3

H1 H2

H ExB RBK-3

AM Operator- DNA IQ: rf B3K Robot#: 20300

edmh 201 JJ4071 11_6

1.5ml Rack 1 1 .5ml Rack 2 Comments: A9 & 89-plexor only, to be added by operator

empty1

1.5ml Rack 3

Plexor® HY Lot#: 0000042914 Exp Date: 10/22/2013 TE-4 Lot#: 8-51

orientation on deck verified by:

Operator- Quant:

7500 Instrument#:

rf 7500A

10 11 12

1.5ml Rack 4

DNA Standard Lot#: 0000042914 Total Extracted Samples:

631

66 1.5ml Tubes for Transfer Method Verified by: rf

1-

CFDLE Florida Department of Law Enforcement Biology Section

Biomek® 3000 Amplification Worksheet

Plexor® HY Results Autosomal (H) Male (Y)

yes Slope

yes H- {-3.3 to -4.0) Y-·(-3.0 to �3.6)

yes (H -23-25) Y-lntercept (Y-24-26) yes

yes R2 yes

yes Crvalue Std. B & 81 (1 0ng) yes

yes H- (20 to 21 ) Y- (21 to 22} yes

yes Cr value Std. E & E1 (0.08ng) yes

yes H- (28 to 29} Y- (29 fo 30) yes

yes IPC Verified

yes OveraiiiPC Cr Grouped f.lrr- H- (79-81°C) TmValue Y- (81-B3°C)

::.yes IPC Melt CurveT M -(79 81°C)

*lPG out of N/A ,. -v·- ·

range:

� �- Melt (T ml out of N/A . . . . range:

Quant Pit Name: PHY AR30Jan 13 7500A RF

Quant evaluator: EDMH Comments: Std G 11 omitted

.. • .· .. ..

. . ...... . .

.... �Rurce Well

_ __ ,: :.· A1 .... 81 .. � C1

01 E1

... F1 . . G1

H1 A2 82 C2 D2 E2 F2 G2 H2 A3

. . .. ,. . B3 C3 .

. . '!··�: 03 ···

. .' . E3

. -� -

F3 G3 H3 A4

02, A5 - deleted black flags

Plexor® HY Results H Quantity

Sample Name/ Sample ID (ng/J!l)

lsw20120406772 Ex3 0.0000 lsw20120406772 RBQ 0.0006 lsw20120406801 Ex1 1.7800 lsw20120406801 Ex2 0.4000 lsw20120406801 ExS 0.0053 lsw20 120406801 Ex6 0.0170 lsw20120406801 Ex? 0.0055 lsw20120406801 Ex8 0.0259 lsw20120406801 RBQ 0.0000 lsw20 120406045 Ex1 0.0966 lsw20120406045 RBQ 0.0039 lsw2011 0404779 Ex9.1 25.3000 lsw20110404779 Ex9.2 0.1250 lsw2011 0404779 RBQ 0.0000 lsw20090401384 Ex2.3 9.9900 lsw20090401384 RBK-3 0.0000 lsw20120406645 Ex6 4.8000 lsw20120406645 RBK 0.0000 lsw20 120406801 Ex3 2.0400 lsw20120406801 RBK-FTA 0.0000 lsw20 120406801 Ex4 4.1200 lsw20120406801 RBK 0.0000 lsw20 120406045 Ex2 8.8200 lsw20120406045 RBK 0.0000 lsw20120406771 Ex1.1 13.4000

Y Quantity (ng/�1)

0.0000 0.0000 1.0500 0.0390 0.0007 0.0011 0.0004 0.0006 0.0000 0.0482 0.0033

22.9000 0.0478 0.0000 4.5200 0.0004 2.3300 0.0000 1.1800 0.0000 0.0000 0.0000 6.6200 0.0000 0.0006

[Auto]/M

0.00 0.00 1.70

10.30 7.50

15.40 13.80 41.90 0.00 2.00 1.20 1.10 2.60 0.00 2.20 0.00 2.10 0.00 1.70 0.00 0.00 0.00 1.30 0.00

23848.90

#of

2 3 4 5 6 7 8 9

10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

I D+ Kit Lot #: Exp. Date:

Thermal Cycler:

9700 #: Calibration date:

TE""' Batch #:

----- ---·- Operator: rf j Analyst(s): lsw edmh

Date Run: 30-Jan-13

83K Plate Name: AR30Jan13

1209050

04 Oct 2013

3

1-15 13

B-51

- -

Number of Samples to be Amplified: 65

----· --·- ---·---

. . - • • 0

, · - � .

84 C4 04 E4 F4 G4 H4 A5 B5 C5 05

· · · ·· · E5 .. , · F5 · .. G5

:,.,

. . . •

. . . . . -- ·- · · . .

. .

H5 A6 B6 C6 06 E6 F6 G6 H6 A7 B7 C7 07 E7 F7 G7 H7 AB BB CB DB EB FB GB HB A9 B9 C9 09 E9 F9 G9 H9

A10 B10 C10 ..

. 010 E10

.. F10 ·- ·. -. . . G10

. . . . H10 . . . A12 B12

Plexor® HY Results lsw20120406771 RBK 0.0000 lsw20120404249 Ex5 5.3300 lsw20120404249 RBK 0.0000 lsw2012040657B Ex1 7.5000 lsw20120406578 RBK 0.0000 lsw20120406998 Ex1 10.1000 lsw20120406998 RBK 0.0000 lsw20120407000 Ex1 19.5000 lsw20120407000 RBK 0.0000 lsw20120406771 Ex1.2sp 6.0300 lsw20120406771 12.4000 lsw20120406771 0.0000 lsw20120406771 0.0000 edmh 20120407111 1 0.0385 edmh 20120407111 2 0.0660 edmh 20120407111 3 0.0108 edmh 20120407111 4 0.0846 edmh 20120407111 RBQ 0.0000 edmh 20120407113 1 0.0135 edmh 20120407113 2 0.0043 edmh 20120407113 RBQ' 0.0000 edmh 20120402713 4 0.3300 edmh 20120402713 RBQ2 0.0000 edmh 20130400029 1 2.8900 edmh 20130400029 2 0.4370 edmh 20130400029 3 0.0207 edmh 20130400029 4 0.5830 edmh 20130400029 RBQ 0.0000 edmh 20120407111 5 FTA 0.1160 edmh 20120407111 RBQ FTA 0.0073 edmh 20120407111 6 2.9700 edmh 20120407111 RBK 0.0000 edmh 20120407113 3 2.3100 edmh 20120407113 4 0.5540 edmh 20120407113 5 4.6300 edmh 20120407113 RBK 0.0000 lsw20130400493 Ex2 0.0005 lsw20130400493 RBQ 0.0000

#N/A 2 mn2 0.0170 RBQ mn2 0.0000

APC AR30Jan 13 0.2510 ANC AR30Jan13 0.0000

AR30Jan13 Date:

0.0000 1.9500 0.0000 4.6400 0.0000 0.0000 0.0000 0.0000 0.0000 6.0900 0.1040 0.0000 0.0000 0.0051 0.0115 0.0055 0.0502 0.0000 0.0003 0.0005 0.0000 0.2610 0.0000 3.0900 0.4850 0.0095 0.7660 0.0000 0.0241 0.0078 2.1500 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 #N/A

0.0124 0.0000

n/a n/a

0.00 2.70 0.00 1.60 0.00 0.00 0.00 0.00 0.00 1.00

119.90 0.00 0.00 7.50 5.70 2.00 1.70 0.00

48.10 8.50 0.00 1.30 0.00 0.90 0.90 2.20 0.80 0.00 4.80 0.90 1.40 0.00 0.00 0.00 0.00 0.00 0.00 0.00

#N/A 1.40 0.00

nla n/a

26 27 28 29 30 31 32 33 34 35 35 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82

C4 lsw20120404249 Ex5 5.3300 04 lsw20120404249 RBK 0.0000 E4 lsw20120406578 Ex1 7.5000 F4 lsw20120406578 RBK 0.0000 G4 lsw20120406998 Ex1 10.1000 H4 lsw20120406998 RBK 0.0000 85 lsw20120407000 RBK 0.0000 C5 lsw20 120406771 6.0300 05 lsw20 120406771 12.4000 E5 lsw20 120406771 0.0000 F5 lsw20120406771 0.0000 G5 edmh 20120407111 1 0.0385 H5 edmh 20120407111 2 0.0660 A6 edmh 20120407111 3 0.0108 B6 edmh 20120407111 4 0.0846 C6 edmh 20120407111 RBQ 0.0000 06 edmh 20120407113 1 0.0135 E6 edmh 20120407113 2 0.0043 F6 edmh 20120407113 RBQ 0.0000 G6 edmh 20120402713 4 0.3300 H6 edmh 20120402713 RBQ2 0.0000 A7 edmh 20130400029 1 2.8900 B7 edmh 20130400029 2 0.4370 C7 edmh 20130400029 3 0.0207 07 edmh 20130400029 4 0.5830 E7 edmh 20130400029 RBQ 0.0000 F7 edmh 20120407111 5 FTA 0.1160 G7 edmh 20120407111 RBQ FTA 0.0073 H7 edmh 20120407111 6 2.9700 AB edmh 20120407111 RBK 0.0000 BB edmh 20120407113 3 2.3100 CB edmh 20120407113 4 0.5540 DB edmh 20120407113 5 4.6300 EB edmh 20120407113 RBK 0.0000 FB lsw20130400493 Ex2 0.0005 GB lsw20130400493 RBQ 0.0000

A12 APC AR30Jan13 0.2510 B12 ANC AR30Jan13 0.0000

A9 2 mn2 0.0170 B9 RBQ mn2 0.0000

Paae 2

- - -- --- --- -- ---- ----- -- - --- ·--- -

101-1l lnput DNA+ 15j.11 Master Mix= 251-11 Amps

(ID+-1 0 f.d total input DNA)+ (1 01-11 extra volume for robotic pipetting) = 20J.ll total input DNA

Number of Samples to be Amped: 65

Normalization End Column #: 9

P50 Setup is used if volume is between2.0J.I and 10.9J.I

P200 Setup is used if volume is over 11.0f-LI

Verify that TE volumes do not exceed 11 Q.I or 230J.!I

Analyst(s): lsw edmh Date: 30-Jan-13

83K Plate Name: AR30Jan13

TE vol ijl.l) X

1.0

1.0

2.4

1.0

1.0

1.0

1.0 1.0

1.0 1.0

1.0

1.0

1.0

10.0 1.0 6.4

1.0 2.7 1.0

5.5

1.0

8.8

1.0

8.9

1.0

7.1 1.0

10.0

1.0 10.1

1.0

1.0 8.0

8.3

1.0

1.0

- --- - -- -- --- - - - -- - -- --- -- · -- --

83K Plate Name: AR30Jan13 Analyst(s}: P�nP.?

X

1.0

1.0

1.0

1.0

1.0

1.0

1.0

1.0

1.0

1.0

3.9

1.0

1.0

1.0

1.0

1.0

1.0

4.0

1.0

3.1

1.0

6.2

1.0

1.0

1.0

1.0

1.0

1.0

1.0

lsw edmh Date: 30-Jan-13

�,dLM0�11-?-

Ext Pit P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1

D'?f1

Source

Well

A1 81 C1 01 E1 F1 G1 H1 A2 82 C2 E2 F2 G2 H2 A3 83 C3 03 E3 F3 G3 H3 A4 84 C4 04 E4 F4 G4 H4 85 C5 05 E5 F5 G5 H5 A6 86 C6 06 E6 F6 G6 H6 A7 87 C7 07 E7 F7 G7 H7 AS

- ·-

Sample Name

lsw201 20406772..;.;Ex3 lsw20 1 20406772_RBQ lsw201 20406801 _Ex1 lsw201 20406801 _Ex2 lsw201 20406801 _Ex5 lsw201 20406801 _Ex6 lsw201 20406801 _Ex7 lsw201 20406801 _Ex8 lsw201 20406801 _R8Q lsw201 20406045_Ex1 lsw201 20406045_R8Q lsw201 1 0404779_Ex9.2 lsw201 1 0404779_R8Q lsw20090401 384_Ex2.3 I sw2009040 1 384_RBK-3 lsw201 20406645_Ex6 lsw201 20406645_RBK lsw201 20406801 _Ex3 lsw201 20406801 _RBK-FTA lsw201 20406801 _Ex4 lsw201 20406801 _R8K lsw20 1 20406045 _Ex2 lsw201 20406045_R8K lsw201 20406771 _Ex1 .1 lsw201 20406771 _R8K lsw201 20404249_Ex5 lsw201 20404249_R8K lsw201 20406578_Ex1 lsw201 20406578_R8K lsw20 1 20406998 _Ex1 lsw201 20406998_R8K lsw201 20407000_R8K lsw201 20406771 _Ex1 .2sp lsw201 20406771 _Ex1 .2epi lsw201 20406771 _R8Qsp lsw201 20406771 _R8Qepi edmh_201 204071 1 1 _1 edmh_201 204071 1 1 _2 edmh_201 204071 1 1 _3 edmh_201 204071 1 1 _ 4 edmh_201 204071 1 1 _R8Q edmh_201 204071 1 3_1 edmh_201 204071 1 3_2 edmh_201 204071 1 3_R8Q edmh_201 2040271 3_ 4 edmh_201 2040271 3_RBQ2 edmh_201 30400029_1 edmh_201 30400029_2 edmh_201 30400029_3 edmh_201 30400029_4 edmh_201 30400029_RBQ edmh_201 204071 1 1 _5_FTA edmh_20120407111_RBQ_FTA

edmh_201 204071 1 1 _6 edmh_201 204071 1 1 _RBK

AR �n l!ln1�

- · -

Norm Pit P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3

· · - -

New Well

A1 81 C1 01 E1 F1 G1 H1 A2 82 C2 02 E2 F2 G2 H2 A3 83 C3 03 E3 F3 G3 H3 A4 84 C4 04 E4 F4 G4 H4 A5 85 C5 05 E5 F5 G5 H5 A6 86 C6 06 E6 F6 G6 H6 A7 87 C7 07 E7 F7 G7

vol (ul)

0.0 0.0

2.0 3.8 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 2.0 0.0 2.0 0.0 2.0 0.0 2.0 0.0 2.0 0.0 2.0 0.0 2.0 0.0 2.0 0.0 2.0 0.0 0.0 2.0 2.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 4.5 0.0 2.0 3.4 0.0 2.6 0.0 0.0 0.0 2.0 0.0

AR 30Jan1 3 flV?(

--r--E---

TE _vol Reservoir

0.0 P2 0.0 P2 0.0 P2 0.0 P2 0.0 P2 0.0 P2 0.0 P2 0.0 P2 0.0 P2 4.5 P2 0.0 P2 8.0 P2 0.0 P2 0.0 P2 0.0 P2 0.0 P2 0.0 P2 0.0 P2 0.0 P2 0.0 P2 0.0 P2 0.0 P2 0.0 P2 0.0 P2 0.0 P2 0.0 P2 0.0 P2 0.0 P2 0.0 P2 0.0 P2 0.0 P2 0.0 P2 0.0 P20.0 P2 0.0 P2 0.0 P2 0.0 P2 0.0 P2 0.0 P2 2.3 P2 0.0 P2 0.0 P2 0.0 P2 0.0 P2 0.0 P2 0.0 P2 0.0 P2 0. 0 P2 0.0 P2 0.0 P2 0.0 P2 7.1 P2 0.0 P2 0.0 P2 0.0 P2

- -

TEwell

A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1

...

Av

- -- - -- - --- - · - · -- -·- - -

Ext Pit Source

Sample Name Norm Pit New Well Template

TE_vol TE

TEwell Well vol (ul) Reservoir

P1 88 edmh_201 204071 1 3_3 P3 H7 2.0 0.0 P2 A1 P1 CB edmh_201 204071 1 3_ 4 P3 AB 2.7 0.0 P2 A1 P1 DB edmh_201 204071 1 3_5 P3 88 2.0 0.0 P2 A1 P1 E8 edmh_201 204071 1 3_RBK P3 CB 0.0 0.0 P2 A1 P1 F8 lsw201 30400493_Ex2 P3 08 0.0 0.0 P2 A1 P1 GB lsw201 30400493_RBQ P3 E8 0.0 0.0 P2 A1 P1 A1 2 APC_AR30Jan 1 3 P3 F8 6.0 0.0 P2 A1 P1 81 2 ANC _AR30J an 1 3 P3 GB 0.0 0.0 P2 A1 P1 empty P3 H8 0.0 0.0 P2 A1 P1 A9 jtm201 20404427 _2_mn2 P3 A9 0.0 0.0 P2 A1 P1 89 jtm201 20404427 _RBQ_mn2 P3 89 0.0 0.0 P2 A1

P?n AR �n.l::1� AR 30Jan1 3

. .

�

Ext Pit

P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1

.. .- P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1

, P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1 P1

- · -

Source

Well

A1 81 C1 01 E1 F1 G1 H1 A2 82 C2 E2 F2 G2 G2 H2 A3 A3 83 C3 03 E3 F3 G3 G3 H3 A4 A4 84 C4 C4 04 E4 E4 F4 G4 G4 H4 85 C5 C5 05 05 E5 F5 G5 H5 A6 86 C6 06 E6 F6 G6 H6

. - - - - · -- ------ - �

Sample Name

lsw201 20406772_Ex3 1sw201 20406772_R8Q lsw201 2040680 1 _Ex1 lsw201 20406801 _Ex2 lsw201 20406801 _Ex5 lsw201 20406801 _Ex6

· lsw201 20406801 _Ex7 lsw201 20406801 _Ex8 tsw201 20406801 _R8Q lsw20 1 20406045 _Ex1 lsw20 1 20406045 _R8Q lsw201 1 0404779_Ex9.2 lsw201 1 0404779_R8Q lsw20090401 38 4_Ex2.3 lsw20090401 384_Ex2.3 lsw20090401 38 4_R8K-3 lsw201 20406645_Ex6 lsw201 20406645_Ex6 lsw20 1 20406645 _RB K lsw20 1 2040680 1 _ Ex3 lsw201 20406801 _R8K-FT A lsw201 20406801 _Ex4 lsw201 20406801 _RBK lsw201 20406045_Ex2 lsw20 1 20406045 _Ex2 lsw20 1 20406045 _RBK lsw201 20406771 _Ex1 .1 lsw201 20406771 _Ex1 .1 lsw201 20406771 _RBK lsw201 20404249_Ex5 lsw20 1 20404249 _Ex5 lsw20 1 20404249 _RBK lsw201 20406578_Ex1 lsw201 20406578_Ex1 lsw20 1 20406578 _RBK lsw201 20406998_Ex1 lsw201 20406998_Ex1 lsw20 1 20406998 _RBK lsw201 20407000_RBK lsw201 20406771 _Ex1 .2sp lsw201 20406771 _Ex1 .2sp lsw201 20406771 _Ex1 .2epi lsw201 20406771 _Ex1 .2epi lsw201 20406771 _RBQsp lsw201 20406771 _RBQepi edmh_201 204071 1 1 _1 edmh_201 204071 1 1 _2 edmh_201 204071 1 1 _3 edmh_201 204071 1 1 _ 4 edmh_201 204071 1 1_RBQ edmh_201 204071 1 3_1 edmh_201 204071 1 3_2 edmh_201 204071 1 3_R8Q edmh_201 2040271 3_4 edmh_201 2040271 3_RBQ 2

n.-,nn AD ':U11-:n1'<

- - --

Norm Pit

P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3

P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P:3 P3 P-3 P.3 P3 P3 P3 P3 P3

· - - - -

New Well

A1 81 C1 01 E1 F1 G1 H1 A2 82 C2 02 E2 F2 F2 G2 H2 H2 A3 83 C3 03 E3 F3 F3 G3 H3 H3 A4 84 84 C4 04 04 E4 F4 F4 G4 H4 AS AS 85 85 C5 05 E5 F5 G5 H5 A6 86 C6 06 E6

· Template-

vol (ul)

20.0 20.0 0.0 0.0

20.0

20.0

20.0 20.0 20.0 1 5.5 20.0 1 2. 0 20.0 0.0 0.0

20.0 0.0 0.0

20.0

0.0 20.0 0.0

20.0 0.0 0.0

20.0 0.0 0.0

20.0 0.0 0.0

20.0 0.0 0.0

20.0 0.0 0.0

20.0 20.0 0.0 0.0 0.0 0.0

20.0 20.0

20.0 20.0 20.0 1 7.7 20.0 20.0

20.0 20.0 0.0

P3 F6 iJl 20.0

AR 30Jan1 3

-

-

-

-

-

TE _vol

0.0 0.0

45.5 1 6.3 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0

98.9 98.9 0.0

63.0 63.0 0.0

52.4 0.0

1 07.9 0.0

8 7.2 87.2 0.0

88.4 88.4 0.0

70.1 70.1 0.0

99.0 99.0 0.0

1 00.0 1 00.0

0.0 0.0

79.4 79.4 81 .7

81 .7

0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0

1 5.5 0.0

TE -Reservoir

P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2 P2

·

--- -

TE well

A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 A1 Av ·

· ----- -- ·- --

-- ----- - ---- - - - -- - - - - -

·-- -- - - - - --- ·

Ext Pit Source Sample Name Norm Pit New Welt

Template TE_vot

TE TEwell

Well vel {ul) Reservoir

P1 A7 edmh_201 30400029_1 P3 G6 0.0 75.1 P2 A1 P1 87 edmh_201 30400029_2 P3 H6 0.0 1 6.6 P2 A1 P1 C7 edmh_201 30400029_3 P3 A7 20.0 0.0 P2 A1 P1 07 edmh_201 30400029_4 P3 87 0.0 1 7.4 P2 A1

P1 E7 edmh_201 30400029_R8Q P3 C7 20.0 0.0 P2 A1 P1 F7 edmh_201 204071 1 1 _5_FTA P3 07 1 2.9 0.0 P2 A1 P1 G7 edmh_20120407111_RBQ_FTA P3 E7 20.0 0.0 P2 A1 P1 H7 edmh_201 204071 1 1 _6 P3 F7 0.0 77.2 P2 A1 P1 AS edmh_201 204071 1 1 _RBK P3 G7 20.0 0.0 P2 A1 P1 88 edmh_201 204071 1 3_3 P3 H7 0.0 59.6 P2 A1 P1 C8 edmh_201 204071 1 3_ 4 P3 AS 0.0 1 7.3 P2 A1 P1 08 edmh_201 204071 1 3_5 P3 88 0.0 60.8 P2 A1 P1 08 edmh_201 204071 1 3_5 P3 88 0.0 60.8 P2 A1 P1 E8 edmh_201 204071 1 3_R8K P3 C8 20.0 0.0 P2 A1 P1 F8 lsw20 1 30400493 _Ex2 P3 08 20.0 0.0 P2 A1 P1 G8 lsw20 1 30400493 _RBQ P3 EB 20.0 0.0 P2 A1 P1 A1 2 APC _AR30Jan 1 3 P3 F8 0.0 1 4.0 P2 A1 P1 81 2 ANC_AR30Jan1 3 P3 GB 20.0 . 0.0 P2 A1 P1 empty P3 H8 0.0 0.0 P2 A1 P1 A9 jtm201 20404427 _2_mn2 P3 A9 ·0.0 0.0 P2 A1 P1 89 jtm201 20404427 _RBQ_mn2 P3 89 0.0 0.0 P2 A1

yJ-(f

AR �n.l�n1�

(FDLE

Instrument ID:

Capillary Array Lot:

Array Count or Date:

POP4 Lot:

Buffer Lot:

Florida Department of Law Enforcement

Biology" Section

3130x/ Plate Record . };_,· :�---�--Biomek 2000 Operator: rf

Analyst(s): lsw edmh

Date: 31-Jan-13

Plate Record Name: AR 30Jan13 Biomek 2000 Method: 61-75

3130xiB ID+ Ladder Lot: 1205041 32A06704 Yfiler Ladder Lot:

7 Formamide Lot: 1202160 1209121 GS 600 Lot: 1207049 1209410 Denature: TC#3@ 95d

�io·s� �W�if�

../=Used V- "'1-' I 1--..J

A1 A2 81 82 C1 C2 01 02 E1 E2 F1 F2 G1 G2 H1 H2 A3 A4 83 84 C3 C4 03 04 E3 E4 F3 F4 G3 G4 H3 H4 A5 AS 85 66 C5

05 06 E5 E6 F5 F6 G5 G6 H5 HS

10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+

10+ Ladder lsw20120406772 Ex3 lsw20120406772 RBO lsw20120406801 Ex1 lsw20120406801 Ex2 lsw20120406801 Ex5 lsw20120406801 Ex6 lsw20120406801 Ex7 lsw20120406801 Ex8 lsw20120406801 RBQ lsw20120406045 Ex1 lsw20120406045 RBQ lsw20 11 0404 779 Ex9 .2 lsw2011 0404779 RBQ lsw2009040 1384 Ex2.3 lsw20090401384 RBK-3 10+ Ladder lsw20120406645 Ex6 lsw20120406645 RBK lsw20120406801 Ex3 lsw20120406801 RBK-FTA lsw20120406801 Ex4 lsw20120406801 RBK lsw20120406045 Ex2 lsw20120406045 RBK lsw20 120406771 Ex 1.1 lsw20120406771 RBK lsw20120404249 ExS lsW20120404249 RBK lsw20120406578 Ex1 lsw20120406578 RBK lsw20120406998 Ex1 10+ Ladder lsw20120406998 RBK lsw20120407000 RBK lsw20120406771 lsw20120406771 Ex1.2epi lsw20120406771 lsw20120406771 RBQepi edmh 20120407111 1 edmh 20120407111 2 edmh 20120407111 3 edmh 20120407111 4 edmh 20120407111 RBQ edmh 20120407113 1 edmh 20120407113 2 edmh 20120407113 RBQ edmh 20120402713 4 !?

Pace 1

A7 ID+ ID+ Ladder AS ID+ edmh 20120402713 RBQ2 87 ID+ edmh 20130400029 1 88 ID+ edmh 20130400029 2 C7 ID+ edmh 20130400029 3 CB ID+ edmh 20130400029 4 07 ID+ edmh 20130400029 RBQ DB ID+ edmh 20120407111 5 FTA E7 ID+ edmh 20120407111 RBQ FTA EB ID+ edmh 20120407111 6 F7 ID+ edmh 20120407111 RBK FB 10+ edmh 20120407113 3 G7 10+ edmh 20120407113 4 GB ID+ edmh 20120407113 5 H7 10+ edmh 20120407113 RBK HB 10+ lsw20130400493 Ex2 A9 10+ 10+ Ladder

A10 ID+ lsw20130400493 RBQ 89 10+ APC AR30Jan13 810 ID+ ANC AR30Jan13

C9 10+ no C10 10+ 2 mn2 plexor 09 10+ RBQ mn2 plexor

010 10+ no E9 10+ no sample

E10 10+ no sample F9 10+ no

F10 10+ no sample G9 10+ no sample

G10 10+ no sample H9 10+ no sample

H10 10+ no sample A11 ID+ A12 10+ 811 10+ 812 10+ C11 10+ C12 ID+ 011 10+ 012 10+ E11 10+ E12 10+ F11 10+ F12 ID+ G11 ID+ G12 10+ H11 10+ H12 ID+

./=Used Paae 2

Florida Department of Law Enforcement Biology Section

RUN ANALYSIS FORM Analyst Run Folder. fW. S !r.:o:> Location: !-'\. � t.SN Case Number(s) or Sample Set

• GS600 present and correctly labeled

• All ladders have correct allelic calls

• Alleles above threshold/below max rfu, peak heights within ratio

• All controls have correct results (Positive/Negative/Blanks)

Comments:

� 0 \� bL-\ 6 �OL\S \ �

Original in first case listed

Date

Applied B1osystems GeneMapper 10 v3.2.1

31308 AR 30Jan131sw

File Name Panel A02 002 lsw20120406772 Ex3 0 1.fsa Ex3 0 Plus Panels v1

90

90

60

150

A02 ·ooz lsw2.0120406772 Ex3 o 1.fsa

90 150

90

60

210

270

330

390

Ex3 0 Plus Panels v1

210

. .

270 330 390

A02 002 lsw20120406772 Ex3 0 1.fsa lsw20120406772 Ex3 0 ldentifiler Plus Panels v1

90 150

90

60

30

Ao2 002 lsw20120406772 Ex3 0 1.fsa

90 150

210 270 330 390

lsw20120406772 Ex3 0 ldentifiler Plus Panels v1

210 270 330 390

801 003 lsw20120406772 RBQ 0 1.fsa RBQ 0 Plus Panels v1

90 150 210 270 330 390

90

60

30

Mon Feb 11,2013 01:57PM, EST Printed by: lsw Page 1 of 2

Applied B1osystems GeneMapper ID v3.2.1

31308 AR 30Jan 13 lsw

File Name Panel 801 003 lsw20120406772 RBQ 0 1.fsa lsw20120406772 RBQ 0 ldentifiler Plus Panels v1

90

90

60

150 210 270 330 390

801 003 lsw20120406772 RBQ ·o 1.fsa lsw20120406772 RBQ 0 ldentifiler Plus Panels v1

90 150 210 270 330 390

90

60

30

801 003 lsw20120406772 RBQ o 1.fsa lsw20120406772 RBQ 0 ldentifiler Plus Panels v1

90 150 210 270 330 390

90

60

Mon Feb 11,2013 01:57PM, EST Printed by: lsw Page 2 of 2

Applied Biosystems GeneMapper 10 v3.2.1

File 810 ·oo4 ANC AR30Jan13 1.fsa

31308 AR 30Jan13 lsw

ANC AR30Jan13

Panel Plus Panels v1

90

60

30

105 175 245 315

385

810 004 ANC AR30Jan13 1 .'fsa ANC AR30Jan13 ldentifiler Plus Panels v1

,;;- -;::;;�D�Sj 105 175 245 315 385

810 004 ANC AR30Jan13 1.fsa ANC AR30Jan13 ldentifiler Plus Panels v1 ' ' '

105

810 004 ANC AR30Jan13 1.fsa

105

Mon Feb 11,2013 02:12PM, EST

175

175

245 3 15

I ANC AR30Jan13

245 3 15

20120406772 Printed by: lsw

385

ldentifiler Plus Panels v1

385

Page 1 of 1

·FDLE Florida Department of Law Enforcement

Jacksonville Regional Operations Center 921 N. Davis Street, Building E Jacksonville, Florida 32209-683 8 1-800-226-6481

Rick Scott, GJvernor Pam Bond� Attorney General Jeff Atwater, Chief Financial Officer

Gerald M. Bailey Commissioner

Adam Putnam, Commissioner of Agriculture

TO:

www.fdle.s tate.fl.us

LABORATORY REPORT February 12, 2013

Sheriff John H. Rutherford Jacksonville Sheriffs Office

501 East Bay Street Jacksonville, FL 32202-2975

FDLE NUMBER: SUBMISSION: AGENCY NUMBE R:

20120406772 1 and2 12-0853093

ATTN: M. A. Musser 61139 SUBPOENAS PERTAINING TO THIS CASE SHOULD REFER TO THE FDLE NUMBER.

VICTIM(S): Jordan Russell Davis

SUBJE CT(S): Michael David Dunn

0 FFENSE(S): Death Investigation Duval County

11/23/2012

L. SukHan Warf Crime Laboratory Analyst Biology Section

RE FE RENCE :

This report references evidence submited to the Florida Department of Law Enforcement on December 06, 2012 and on December 18, 2012 by Maurice Lawrence. This report may contain conclusions, opinions, and/ or interpretations made by the author.

E VIDENCE :

FDLE Item#

3 8 9 10

Agency E xhibit#

1-9 6-2 6-3 6-4

RE SULTS:

Description

DNA swab from shell casings at scene DNA swabs from shell casings recovered from suspect vehicle DNA swab from top of magazine recovered from suspect vehicle DNA swab from live rounds recovered from suspect vehicle

STR DNA analysis was performed on a sample from Exhibit 3 utilizing the AmpF lSTR Identif.tler Plus PCR Amplification Kit.

No DNA results were obtained from Exhibit 3 .

STR DNA analysis was not performed on Exhibits 8, 9 and 10 .

111 111111111111111111111111111 11 1111 1 111 Page 1 of2

Service • Integrity • Respect • Quality

FDLE NUMBER: 20120406772 Submissions: 1 and 2

REMARKS:

· · · · · · The·submited evidence· is available-Jor retrieval orreturatthe earliest opportunity; Thls evidence ·includes DNA extracts. It is recommended that all DNA extracts be stored frozen.

Abbreviations that may appear in this report are: CO DIS Combined DNA Index System; DNA deoxyri bonucleic acid; PCRPolymerase Chain Reaction; STR short tandem repeat; Y -STR male spe cific short tandem repeat; FBI Fede ral Bureau of Investigation; and JFS Journal of Forensic Sciences.

Questions regarding this report should be addressed to: jrocbiology@fdle. state.fl.us.

Page� of2

ASCLDILAB-International

STATEMENT OF QUALIFICATIONS Name Lucretia SukHan W arf Date sno12o13

Florida of Law Enforcement I Jacksonville Center

Job Title Crime

Indicate all disciplines in which you do casework:

D Drug Chemistry D Toxicology

D Firearms/Tool marks IX] Biology

D Trace Evidence D Questioned Documents

D Latent Prints D Crime Scene

D Digital & Multimedia Evidence

L ist all category(ies) of testing in which you do casework:

and DNA.

Breath Alcohol Calibration Categories

D Toxicology- Breath Alcohol Measuring Instruments (The work of the laboratory MUST include calibration certificates-do not check the box if work is limited to breath/alcohol testing)

D - Breath Alcohol Calibration Reference Material

Education: List all higher academic institutions attended (list high school only if no college degree has been attained)

Institution Dates Attended Major Degree

ofNorth Florida 8/1998 - 5/2002 B. S. of Florida 5/2003 - 12/2005 Forensic DNA & Serology M.S.

Other Training: List continuing education, workshops, in-service and other formal training received. Please include the course title, source and date of the training.

University of North Florida, Biochemisty FDLE Forensic Technologist Serology and DNA training program FDLE Crime Laboratory Analyst Serology and DNA training program Paternity Index DNA Statistics, SAFS, MAFS, MAAFS, CAS Joint meeting Applied Biosystems GeneMapper ID Workshop, SAFS, MAFS, MAAFS, CAS Joint meeting GeneMapper ID Training, FDLE 3130xl Genetic Analyzer training, FDLE Statistics in the Courtroom, 5th Annual Advanced DNA Technology Workshop, The Bode Technology

Group, Inc. Beckman Biomek 3000 and 2000 rpbotics training with Promega's DNA IQ extraction system, FDLE HID University 3130xl and Genemapper ID training, Applied Biosystems FBI DNA Auditor Workshop DNA Identifications for Missing Persons and Disasters SAFS, MAFS, MAAFS, SW AFS Joint meeting YSTRs: Science and Statistics SAFS, MAFS, MAAFS, SW AFS Joint meeting ASCLD/LAB International Preparation Course, ASCLD/LAB Plexor HY and PowerPlex Y training, Promega

ASCLD/LAB-lnternational Statement of Qualifications Approval Date: August 3, 2012 Approved By: Executive Director

Page 1 of2 Effective Date: August 3, 2012

AL-PD-3018-Ver 3.0

Plexor HY quantitation training, FDLE Y filer training, FD LE Identifiler Plus training, FDLE

Courtroom Experience: List the discipline/category(ies) of testing in which you have qualified to testify as an expert witness and indicate over what period of time and approximately how many times you have testified in each.

Serology (since 2005):25 DNA (since 2005) :35

Professional Affiliations: List any professional organizations of which you are or have been a member. Indicate any offices or other positions held and the date(s) of these activities.

Employment History: List all scientific or technical positions held, particularly those related to forensic science. List current position first. Be sure to indicate employer and give a brief summary of principal duties and tenure in each position.

Job Title Crime Tenure 2/2005 - FDLE

Provide a brief of duties: evidence STR-DNA of evidence and courtroom

Job Title Crime Tenure Crime

FDLE Provide a brief of duties : In to learn STR-DNA of evidence and courtroom

Job Title Forensic Tenure 8/2002 - 5/2004

FDLE Provide a brief of duties : General lab support, instrument maintenance and operation, analyst support, sweep cases for trace evidence, general serology evidence

Job Title Volunteer Tenure 3/2002 - 8/2002

FDLE Provide a brief description of duties: Evidence and office

Job Title Tenure

Provide a brief of duties :

Other Qualifications: List below any scientific publication and/or presentation you have authored or co-authored, research in which you are or have been involved, academic or other teaching positions you have held, and any other information which you consider relevant to your qualification as a forensic scientist. (Use additional sheets if necessary.)

FDLE Plexor HY and Y-filer internal validation team

ASCLD/LAB-lnternational Statement of Qualifications Approval Date: August 3 , 20 1 2 Approved By: Executive Director

Page 2 of2 Effec-tive Date: August 3, 20 1 2

AL-PD-301 8-Ver 3 .0

tj_ Full Member

Form

IF Associate Member, Name of Full Member Sponsor

Badge Name tY'-:C. \·L-£. .;:' 6 4- -1 -4 ...

Drivers License No . .) D <;

Newsletter by Email? Yes .J No Email Address :

I �- Cbc"'­NRA Membership Number

Sign me up for the NRA

I wil email my NRA number Initiation F ee 1, ()6 Yearly Dues

NRA Fee � s Total ?>�1

. Vice President.

Treasurer

Executive Officer

I certify that I am of good repute; that I am not a member of any organization or group having as its purpose the overthrow or disruption by force or viol ence the Government of the United States of America or any of the political subdivisions; that I have never been convicted of a crime of violence; that if admitted to membership, I will fulfill the obligations of good sportsmanship and good citizenship . I hereby attest the above statements are true, and any falsehood will deprive me of membership. I understand that I must remain an NRA member for the duration of my club membership. I also understand that by signing this application I am consenting to a possible background check.

Applicant Signature

FOR CLUB USE ONLY

Orientation Attended: � Date Orientation Lead Signature

Page � Revised 1 0/2 1109

RELEASE AND HOLD HARMLESS AGREEMENT In consideration for participating in club activity sponsored or organized by the Port Malabar Rifle and Pistol Club, Inc., the undersigned Participant HEREBY AGREES TO WAIVE, RELEASE, DISCHARGE, AND HOLD HARMLESS the Port Malabar Rifle and Pistol Club, Inc. from any and all claims for damages for death, personal injury, or property damage which they may have or which they may hereafter accrue as a result of participation in any Port Malabar Rifle and Pistol Club, Inc. program or related activity and events including, but not limited to unorganized practice, organized practice, training and instruction and organized shooting events.

By signing this Release and Hold Harmless Agreement, the undersigned recognizes that the sport of rifle, pistol, and shotgun shooting or related activities present certain inherent dangers that cannot be eliminated even by the exercise of reasonable care. As such, the undersigned Participant hereby expressly assumes the risk that is inherent to the sport of rifle, pistol, and shotgun shooting.

As a requisite condition to the Participant being allowed to attend and participate in any Port Malabar Rifle and Pistol Club, Inc. sponsored event or trip, the undersigned Participant hereby acknowledges that the State of F lorida has the most significant relationship with the parties to this Agreement rather then the destination state or providence. As such, the parties hereto expressly agree to F lorida as the Choice of Law and Choice of Forum to any claim, suit, or controversy that may arise by and between the p arties or any of its related affiliates, and further agree that any action, suit, or claim made by the Participant must be brought in the State of Florida as the proper Venue having exclusive subj ect matter jurisdiction to -any such claim, demand, or suit. To effectuate this expressed Choice of Law, Choice of Forum and V enue provision, the undersigned hereby agrees that the state or providence where the injury or cause of action arose shall not have either subject matter jurisdiction or in personam jurisdiction over the parties hereto .

By signing this Release and Hold Harmless Agreement, the undersigned Participant assumes the risk inherent to rifle, pistol, and shotgun shooting, and thereby consents to the Participant's p articipation in Port Malabar Rifle and Pistol Club, Inc. programs and related activities, and further acknowledge that he/she understands that all risks, whether known or unknown, are expressly assumed by the undersigned, and that all claims, whether known or unknown, are expressly waived in advance.

In addition to the foregoing, the undersigned Participant hereby expressly agrees and recognizes that the Port Malabar Rifle and Pistol Club, Inc., through its individual Match Directors, Match Chairmen, Range Officers, judges and referees, officers, directors, agents, individuals or entities perform volunteer services, and as such, said entities and/or individuals are immune from liability as volunteer leaders, assistants, and organizers.

Arbitration: In further consideration for being allowed to participate in any activities sponsored by the Port Malabar Rifle and Pistol

Club, Inc. the undersigned Participant recognizes and agrees that this contract involves interstate commerce which is necessarily controlled by the Federal Arbitration Act (9 USC § 2), and therefore, pursuant to the Federal "Arbitration Act (FAA) as well as the Florida Arbitration Act (Florida Statutes § 682.02) hereby agree to submit to binding arbitration any and all claims, demands, suits, or other disputes that may arise out of this contract, or that may be presented by or on

. behalf of the Participant for resolution

under the Commercial Rules of the American Arbitration Association with the initial filing fees thereof to be paid by the party filing the Demand for Arbitration subject to the schedule of fees then in effect at the time of the filing of a Demand with the American Arbitration Association.

I HAVE READ THE FOREGOING TERMS AND CONDITIONS AND AGREE TO BE BOUND BY ALL OF THE CONDITIO S SET FORTH ABOVE.

Page 1 Revised 1 0/2 1/09

Detective T. A. Stapp #7725 Detective M . A. M usser #61 1 39 (Lead Detective) Detective E. M. Cayenne #6060 Detective T. D. Ol iver #7779

WITNESSES INTERVIEWED BY DETECTIVE CAYENNE

DETAILS OF INVESTIGATION

Friday/1 1 -23-1 2/2000 hours : Detective Cayenne received a phone call from Sergeant Paul advising our team was being called to 8251 Southside Boulevard (Gate Gas Station) in reference to a shooting in the parking lot. Sergeant Pau l advised that when we are done with our other crime scene we could meet at the Gate Gas Station.

Note: Let i t be noted that Detectives Stapp and Cayenne was handling another death investigation and never made it to the crime scene.

F riday/1 1 -23-1 2/231 0 hours: Detective Cayenne spoke with Sergeant Paul and advised him that Detectives Cayenne and Stapp were done with our death investigation where did he need us to respond. Sergeant Paul advised Detective Cayenne to respond to the Homicide Office.

Friday/1 1 -23-1 2/230 hours: Detective Cayenne arrived at the Homicide Office.

Tuesday/1 1 -27 -1 2/1 805 hours : Detectives Cayenne and Stapp responded to the strip mall located at 8221 Southside Boulevard to conduct a canvass of the businesses.

Detective Cayenne spoke with owners or employees of the fol lowing businesses:

U n it 3- Kaluby's Dance Club advised they had video survei l lance i nside the business and outside on the back side. There was no video outside the front of the business.

U n it 5 - The Loop Restaurant advised the business has surveillance inside and views outside the rear of the business. There was no video outside the front of the business.

U n it 1 1 - Tropical Smoothie advised the business has surveil lance inside and views outside the rear of the business. There was no video outside the front of the business.

Detective Cayenne spoke with employee who stated he was working on the n ight of the shooting. He advised he d id not hear any gunshots. remembered a police car had pul led over a veh icle in the Publix parking lot. The next th ing he remembered were multiple police cars responding to the scene. He did not learn of the shooting until later that night.

Detective Cayenne spoke with witness who was working on the night of the incident. did not hea r any gunshots.

S U P Page 2 of 4 Date Printed: 1013/2013 J O H N H . RUTH E RFORD, S H E RI F F 2012 853093-9 S U P

Case Cleared CLEARED BY ARREST (DETECTIVE)

CRIME ANAL YSIS Aggravated Assault/Murder: ARGUMENT Type of Weapon: HANDGUN (DISCHARGED) Forced Entry: NOT APPLICABLE Structure Occupancy Code: NOT APPLICABLE Number of Premises Entered:

Location Type: Gas/Service Station(business that services vehicles) Incident Occurred Outside th is Location Incident Occurred In the Parking Lot at this Location: Yes Number of Vehicles Recovered: Number of Arrested: 1 School Name: School Number

MISCELLANEOUS: Is Offense Related to Domestic Violence: No If yes, were Children under 18 Present: No If No is it Domestic Related: NO Is there additional information included on a continuation report: No Are there other Pertinent Reports: Yes In your opinion is there sign(ficant reason to believe that the crime can be solved by a patrol follow-up investigation? No Neighborhood Canvass Conducted: Yes Case Information Card Left With: N.A. If Other (Name Address):

Address: Apt./Lot #: Sub-Sector:

City: State: Zip:

Taz: Crossstreet:

Home Phone # Bus. Phone # Ext.

Cell Phone # Cell Phone Provider E-mail

Is Vagrant? NO

Investigative Time: #1 0 Hours: 1 0 Minutes: 0 Cost Amount: $482.40

Detective Called To the Scene:M.A.MUSSER #61 1 39 Evidence Technician Called to the Scene: #0 Reporting Officers: E.M.CAYENNE

Approving SupervisorM .A. PAUL Division: DETECTIVE Unit: HOMICIDE

NA: ( 1 NA: [ 1

ID #6060 1D #0 ID #6097

Not(fied: [ X 1 Assigned By HQ: [ X 1

8/1 5/201 3 1 3 :05

S U P Page 4 of 4 Date Printed: 1 01312013 J O H N H . RUTH E RFORD, S H E RI F F 2012 853093-9 S U P

at the Burger King located near the i ntersection of Beach Boulevard and St. Johns Bluff.

Th is report documents all ind ividual investigative efforts in this case that were conducted by Detective Oliver. Refer to Detective M usser's report for more detai ls of the investigation.

Clearance Status: CLEARED BY ARREST Clearance Code: ADULT Date case was cleared: 1 1 /24/201 2 Number of Cases Cleared 1 Case Cleared CLEARED BY ARREST (DETECTIVE)

CRIME ANAL YSIS Aggravated Assault/Murder: ARGUMENT Type of Weapon: HANDGUN (DISCHARGED) Forced Entry: NOT APPLICABLE Structure Occupancy Code: NOT APPLICABLE Number of Premises Entered:

Location Type: Gas/Service Station(business that services vehicles) Incident Occurred Outside th is Location Incident Occurred In the Parhng Lot at this Location: Yes Number of Vehicles Recovered: Number of Arrested: 1 School Name: School Number

MISCELLANEOUS: Is Offense Related to Domestic Violence: No Jfyes, were Children under 18 Present: No If No is it Domestic Related: NO Is there additional information included on a continuation report: No Are there other Pertinent Reports: Yes In your opinion is there significant reason to believe that the crime can be solved by a patrol follow-up investigation? No Neighborhood Canvass Conducted: Yes Case Information Card Left With: N.A. If Other (Name Address):

Address: Apt./Lot #: Sub-Sector:

City: State: Zip:

Taz: Crossstreet:

Home Phone # Bus. Phone # Ext.

Cell Phone # Cell Phone Provider E-mail

Is Vagrant? NO

Investigative Time: #1 1 Hours: 0 Minutes: 0 Cost Amount:

Detective Called To the Scene:M.A.MUSSER #61 1 39 Evidence Technician Called to the Scene: #0 Reporting Officers: T. D. 0 LIVER

Approving Supervisor M .A. PAUL Division: DETECTIVE Unit: HOMICIDE

NA: [ 1 NA: [ 1

ID #7779 ID #0 ID #6097

Notified: [ X 1 Assigned By HQ: [ X 1

8/1 9/201 3 1 4:23

S U P Page 3 of 3 Date Printed: 10/312013 J O H N H . RUTH E RFORD, S H E RI F F 2012 853093 10 S U P

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Ok J enough filler, on to the real news. Thought of you yesterday after all of this and said man Mike waul probably really enjoy this article and the talk back section.

e Are Watching You Act, propo sed by Democratic Massachuset Rep. Michael Capuano and Rep lican North Carolina Rep. Walter Jones, will keep corporations from using digiW vide<>.t" recor ers (DVRs) to collect and share your personal information.

Kine Polygon reports. The bill would require the consumer to provide prior consent before info ation is collected, and the operator of the would have to provide access to how the data s collected, said a press release on the House's website.

Infr ed cameras and installed in the DVRs would be used to collect the surveillance info ation. If not given a consumer 's permission to be filmed, the would be forc�d to offer other option that does not film their consumers but that is "otherwise identical in all aspects." If a c nsumer does allow the information to be collected, a bright red message that reads "WE ARE WAT HING YOU" must be flashed across the screen at all times while recording.

The ouse's press release explains the teci.Jx.• companies' interest in this technology: Essentially, they want to better and more quickly figure out what to sell you- a way to "super-target ads," Capuano said . "If s meone is watching a favorite program while cleaning the kitchen, ads for products that will make appli ces shine may show up. If a couple is talking about where to dine over the weekend, or enjoying a c chy snack while watching the Stanley Cup finals, their television may serve up food suggestions. The atent application even goes so far as to suggest that if a couple is fighting, an advertisemen.tt"' asso iated with relationship counseling might be selected for them," it said.

Jone and Capuano both offered quotes in their press release. In li ht of the government's "unfortunate history of secretly collecting private citizens' information from we must ensure that safeguards are in place to protect Americans' rights," Jone said. Cap o stressed that though "this may sound preposterous," it is "neither a joke nor an exaggeration,"

and ged consumers to consider the impact this sort of information-sharing will have on their personal

about what you do in the privacy of your own home and then think about how· you would feel g that information with your cable company, their advertisers and your

ress release concluded with the message that this is · a matter of awareness, and having a choice iri atter.

minimum, consumers should have the option of saying no to being watched."

d it interesting . At first I thought it was another over reach of govenunent then I looked at the Dire TV DVR box and re-read the article. I realized this wasn't written specifically for the Xbox One. This was written and aimed at Cable companies with DVR's. I had already read the comments section at s point and most of the comments were along the lines of . . . .

er solution, don't buy an Xbox. There i s n o need for a law. "

an, this is so over the top"

I I ..

Then e post I replied to . . .

"Micr soft and other companies will make it al-or-none . Either you enable the earner� to transmit back to the orne office, or you don't use the camera for anything. I still don't see the need for a federal law. If don't want to be surveilled, don't buy an Xbox. Sony or Nintendo will come out with a compttitive product that is not so intrusive. Fre market win."

My I too this until I looked at my cable box. What if cable companies decided to do thisl We're very Ilmited in what cable companies you can use. Without any oversight and very little effort, the cable �ompanies could work together and push that out. Most people will do without a gaming

conso�es, how many would actually say no to cable? I

Somefne else replying to me You :must admit though, the principle is the exact same no matter how much you want the product. /1 My Reply back

While I do agree the federal government should be very limited in its interference with I anything, this is not one of those cases. The government has a job of protecting your right to

I privacy. It is one of the few things the government is supposed to do. I have no problem with I the government telling corporations that they can not punish us if we don't allow them to spy on

I us.

I I

The same guy replying back to n1e The government has no such duty to protect your right of privacy in voluntary transactions

between private citizens/entities. You have no more right to an xbox without a camera than a right to vegetables without genetically modified organisms . Nor do you have a right to cable.

And what if you did? Take it to the logical conclusion, what other freely produced/purchased

items would you then have the right to force (by the State, legislature) producers to make according to your whim? I don't like being spied on, so I won't buy the product . That's where the analysis stops.

My reply back

It does when it is the one using said corporations to spy on the citizens. Cable companies are

also not entirely private entities. They are highly subsidized by big government. If they were

truly private entities many non..;spying ones would pop up as soon all the major ones started

spying. Every home would have a choice of 2 or more cable providers but that isn't the case for most people.

I don't eat GMO's but would agree the government has no right to make companies label GMO products . Free Market works just fine because non-GMO foods make sure it is written al over. As a consumer you're left with many choices.

Cable companies are completely different. It's about the control of information. If cable companies tomorrow could tell you that you either put in a camera or they stop providing you

TV /Internet access, you're at a serious disadvantage in life. �th the way things are going, that isn't completely far-fetched.

In a truly free society we wouldn't need this, but that's not where we are in history.

His Final Reply back to me.

exists through State action. So maybe it is a different analysis with cable. But that is one of a very few industries where that is the case. (Thankfully)

It as an interesting back and forth. I think I would actually argue that even in a case like the Xbox One, as a consumer you should have the option of buying that hardware without the spy cam on it. I thi at some point, a conversation has to take place about the true role of the constitution and the fed ral government. I'm one of those that believes the federal governments j ob is to protect the bill of rig Sometimes the few need to be protected from the masses when it comes to the bill of rights. If 80° o of the population decided tomorrow that they don't care about their right to privacy and every co pany out there started to put a camera, microphone, and internet connection on their devices then req ·red you to have those parts operational in order to use the device, what happens to the 20% of pe le that don't want their privacy stolen from them? I'm talking about a stove, a microwave, a

·gerator, your cable box, your AC unit. This seems far fetched for now, but 20-3 0 years from now, I do 't know that it will be as far fetched. I completely understand that in a truly free republic, this wo ld never happen. But that's not what we have. We have a society filled with idiots that don't care ab t any of their rights and will give them up for the latest gadget and a government that is loving the}r new abilities to spy on the people. I think people need to be cautious when it comes to the kind of leg slation. As I pointed out earlier in my replies, I don't think foods that contain GMO's should have to e labeled. I'm more concerned about a person's right to privacy in their home.

Fe eral Nullification efforts mounting in states . . . . Im gine the scenario: A federal agent attempts to arrest someone for illegally selling a machine gun. Ins ead, the federal agent is arested - charged in a state court with the crime of enforcing federal gun la s . Farfetched? Not as much as you might think. Th scenario would become conceivable if legislation passed by Missouri's Republican-led Legislature is s�gned into law by Democratic Gov. Jay Nixon.

Missouri legislation is perhaps the most extreme example of a states' rights movement that has be spreading across the nation. States are increasingly adopting laws that purport to nullify federal

s -. setting up intentional legal conflicts, directing local police not to enforce federal laws and, in rar cases, even threatening criminal charges for federatagents who dare to do their jobs.

An Associated Press analysis found that about four-fifths of the states now have enacted local laws that dir ctly reject or ignore federal laws on marijuana use, gun control, health insurance requirements and ide standards for driver's licenses. The trend began in Democratic leaning California with a

1 9 6 medical marijuana law and has proliferated lately in Republican strongholds like Kansas, where Go . Sam Brownback this spring became the frrst to sign a measure threatening felony charges against fed ral agents who enforce certain firearms laws in his state.

So e states, such as Montana and Arizona, have said "no" to the feds again and again - passing es' rights measures on all four subjects examined by the AP - despite questions about whether their

"n " caries any legal significance.

"It see ·s that there has been an uptick in nullification efforts from both the left and the right," said Adam a professor at the University of California at Los Angeles who specializes in consti law.

Yet ' e law is clear - the supremacy clause (of the U.S. Constitution) says specifically that the feder laws are supreme over contrary state laws, even if the state doesn't like those laws," Wm.k.ler added

over state ones hasn't stopped some tates from flouting those federal laws - sometimes successfully.

20 states now have medical marijuana laws allowing people to use pot to treat chronic pain and other ' lments - despite a federal law that still criminalizes marijuana distribution and possession. Cedin ground to the states, President Barack Obama's administration has made it known to federal prose utors that it wasn't worth their time to target those people.

authorities have repeatedly delayed implementation of the 2005 Real ID Act, an anti-terrorism law set stringent requirements for photo identification cards to be used to board commercial flights or r federal buildings. The law has been stymied, in part, because about half the state legislatures have pposed its implementation, according to the National Conference of State Legislatures .

Abou 20 states have enacted measures challenging Obama's 201 0 health care laws, many of which speci tcally reject the provision mandating that most people have health insurance or face tax penalties begi ing in 20 14.

After Montana passed a 2009 law declaring that federal fireans regulations don't apply to guns made and pt in that state, eight other states have enacted similar laws.

The upreme Court ruled in 1 997 that local police could not be compelled to carry out provisions of a feder gun control law. But some states are now attempting to take that a step further by asserting that certa n federal laws can't even be enforced by federal authorities .

A ne Kansas law makes it a felony for a federal agent to attempt to enforce laws on guns made and own d in Kansas. A similar Wyoming law, passed in 201 0, made it a misdemeanor. The Missouri bill also ould declare it a misdemeanor crime but would apply more broadly to all federal gun laws and reg ations - past, present, or future - that "infringe on the people's right to keep and bear arms."

U.S . General Eric Holder sent a letter in late April to the Kansas governor warning that the al government is willing to go to court over the nevv lav.'. "Kansas may not prevent federal

emp oyees and officials from caring out their official responsibilities," Holder wrote.

So woul� happen if a local prosecutor actually charges a federal agent for doing his or her job? "Th y're going to have problems if they do it - there's no doubt about it," said Michael Boldin, exe tive director of the Tenth Amendment Center, a Los Angeles-based entity that promotes states' righ s . "There 's no federal court in the country that's going to say that a state can pull this off."

Yet tates may never need to prosecute federal agents in order to make their point. If enough states resi , "it's going to be very difficult for the federal government to force their laws down our throats," Bol in said.

Mis ouri 's governor has not said whether he will sign or veto the bill nullifying federal gun laws. Me while, thousands of people have sent online messages to the governor's office about the legi lation.

Signi g the measure "will show other states how to resist the tyrany of federal bureaucrats who want to ro you of your right to self-defense," said one message, signed by Tim and Arlena Sowash, who

gun shop in rural Stover, Mo. ·

e? dang time! People need to realize that the federal government does not have our best interest in If we can't take our country back with elections, maybe we can take parts back with nullification