methodology ir raman spectroscopies
DESCRIPTION
Methodology IR Raman SpectroscopiesTRANSCRIPT
-
(IR SPECTROSCOPY) I Raman (Raman SPECTROSCOPY)
1000 1200 1400 1600 1800
1391
1547
1519
1373
1334
1275
1158
1041
1228
1446
1627
1670 17
32
2nd
deriv
ative
Wavenumber (cm-1)
cA peptide oriented fiber stained with Congo red
ATR
units
2015
. . 1
-
: H
: EINAI MIA STEIN: /
2 . . .
-
/ .
3 . . .
-
/ : : o
=c/
c: (3 x 1010 cm/sec)
4 . . .
-
cm. :
= 1/ = /c
5 . . .
-
/ . : E = h
h: Planck=6,624x10-34 J s
6 . . .
-
/ 3 : A
7 . . .
-
H
~ 4000-8000
8 . . .
-
?
hv1 hv2
hv3
hv1
hv3
9 . . .
-
:
: To , Planck: E = h (, ) . . , . 1.
10 . . .
-
Lambert-Beer
(: )
leII = 00I
l
I
11 . . .
-
2 :
= / 0 %
12 . . .
-
A=Absorbance=
IIA 010log=
0I
l
I
lCIIA ..log 010 ==
Lambert-Beer
(: )
:
13 . . .
-
() Boltzmann
(=0). 1 . .
14 . . .
-
2 ( ) m1 m2.
H ( , ) :
E= Kx2
.
, .
, .
;
m2 k m1
15 . . .
-
orse
1
16 . . .
-
;
m1 m2 .
m1 m2 k
H k. , Hooke. : =1/2 k/ (= ) 1/=1/m1+1/m2 =m1m2/(m1+m2) 17
. . .
-
()
: m1 m2 :
m1>>>>>>>>>>>>>>>>>>>>>m2 m1=m2
: a. 1/=1/+1/m2=1/m2 . =m2 b. 1/=1/m1+1/m2=2/m2 . =m2/2
18 . . .
-
!
, ( , ). Morse , . (-) ( , ).
19 . . .
-
orse
1
20 . . .
-
21 . . .
-
. :
. ,
: 3N-6 3-5 ( )
22 . . .
-
23 . . .
-
IR
:
, , ...
in-situ :
24 . . .
-
( ) .................................................................................................................. ................................................................................................................. ................................................................................................................
:
25 . . .
-
,
26 . . .
-
27 . . .
-
(IR)
1000 1500 2000 2500 3000 3500 4000
A
Wavenumber (cm-1)
vmax
28 . . .
-
: Wavenumber = ( )
v -
1
= (cm-1)
cv =
29 . . .
-
A=Absorbance=
IIA 010log=
leII = 0 0Il
I
lCIIA ..log 010 ==
Lambert-Beer
(: )
(: )
:
30 . . .
-
1000 1500 2000 2500 3000 3500 4000
A
Wavenumber (cm-1)
?
31 . . .
-
32 . . .
-
...
= 1100 - 1800 cm-1
1000 1500 2000 2500 3000 3500 4000
A
Wavenumber (cm-1) 33 . . .
-
1200 1400 1600 1800
1660
1273 13
90
1157 12
28 1450
1531
1628A
Wavenumber (cm-1)
1100 - 1800 cm-1
34 . . .
-
A : 1600-1700 cm-1
: 1500-1600 cm-1 : 1220-1330 cm-1
( )
35 . . .
-
A : C=O
: C-N , - : N-H
36 . . .
-
:
-
-
-
I
1645 - 1660
1620 - 1640
1662 - 1695
1645-1655
II
1546
1520, 1530
III
1295 - 1328
1225 - 1250
1257 - 1288
IR
37 . . .
-
1200 1400 1600 1800
1660
1273 13
90
1157 12
28 1450
1531
1628
A
Wavenumber (cm-1)
38 . . .
-
1000 1500 2000 2500 3000 3500 4000
A
Wavenumber (cm-1)
N-H C-H O-H
3280 cm-1
-: 3290 - 3300 cm-1 - : 3280 3300 cm-1
39 . . .
-
900 1000 1100 1200 1300 1400 1500 1600 1700 1800
A
Wavenumber (cm-1)
1200 1400 1600 1800
1660
1273 13
90
1157 12
28 1450
1531
1628
A
Wavenumber (cm-1)
40 . . .
-
1000 1200 1400 1600 1800
1391
1547
1519
1373
1334
1275
1158
1041
1228
1446
1627
1670 17
32
2nd
deriv
ative
Wavenumber (cm-1)
cA peptide oriented fiber stained with Congo red
ATR
units
2
S : M: W:
41 . . .
-
42 . . .
-
, .
CO2
: 43
. . .
-
44 . . .
-
: M . , , . , , . , Rayleigh.
45 . . .
-
Raman (1928)
O C.V. Raman (laser) , . Raman. , , . Rayleigh Raman , (h= h-). Stokes ( ), (h= h+). - Stokes 46
. . .
-
47 . . .
-
Raman: , ( Rayleigh) ( Raman, ). ( ) .
48 . . .
-
H Rayleigh Raman ( Stokes anti-Stokes) .
Stokes anti-Stokes Boltzman .
Raman Stokes ( laser )
RAMAN
49 . . .
-
600 800 1000 1200 1400 1600 18000,015
0,020
0,025
0,030
Ram
an In
tens
itycA-peptide
925
8948
51
643
75572
4
5975
23
1615
11681
102
1032
1003
1333
1241
1453
1669
Wavenumber (cm-1)
FT-Raman
50 . . .
-
Raman
- -
-
I 1650-1660 1665-1680 1665, 1690 1660-1667
A II 1516, 1545 1535, 1560 (I) 1550-1555
1567
(II) 1545, 1555
1560
A III 1260-1290 1230-1240 1290-1330
51 . . .
-
K (cm-1) 510 S-S (g-g-g)
525 S-S (g-g-t)
540 S-S (t-g-t)
622 Phe
643 Tyr / Phe
759 Trp
830 Tyr
850 Tyr
882 Trp
1003 Phe C-C
1035 Phe
1100-1110 C-N
1171 Tyr
1206 Tyr, Phe
1245 (. -)
1268 (- ??? -)
1341 (-)
1447 CH2
1553 (-)
1611 Tyr, Phe, Trp
1671 I (. -)
2800-3100 C-H /N-H / OH 52
. . .
-
53 . . .
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