meredith rutledge bl6 and cyp2b-null presentation
TRANSCRIPT
2015 Summer Research Presentation
By Meredith Rutledge
Cytochrome P450 (CYPs)
• Hemoproteins (superfamily of proteins containing a heme cofactor)
• Terminal oxidase enzymes in electron chains• More than 21,000 identified CYP proteins
Cytochrome P450 Oxidase
Cytochrome P450 2B6
• Monooxygenase • Catalyze many reactions involved in drug
metabolism and synthesis of cholesterol, steroids and other lipids
• CYP2B localized in endoplasmic reticulum • Expression induced by phenobarbital• Known to metabolize some xenobiotics (anti-
cancer drugs)
Hypothesis: Cyp2b-null mice will be more susceptible to a HFD in comparison to the WT group
Activity Study on Cyp2b-null and BL6 (WT) Female MiceTreatment HFD and ND
HFD caused increase in boldness for WT females
Activity Study on Cyp2b-null and BL6 (WT) Male MiceTreatment HFD and ND
Decrease in total activity due to ND and HFD treatment
Boldness Study on Cyp2b-null and BL6 (WT) Female MiceTreatment HFD and ND
ND treatment increased boldness in WT
Boldness Study on Cyp2b-null and BL6 (WT) Male MiceTreatment HFD and ND
Diet had an effect on the weight gain
Weight Data on Cyp2b-null and BL6 (WT) Female MiceTreatment HFD and ND
Genotype and diet had an effect on weight gain
Weight Data on Cyp2b-null and BL6 (WT) Male MiceTreatment HFD and ND
Fasting Glucose Data on Cyp2b-null and BL6 (WT) Female MiceTreatment HFD and ND
Fasting Glucose Data on Cyp2b-null and BL6 (WT) Male MiceTreatment HFD and ND
Conclusion
• Diet and genotype has an effect on weight gain in males
• Diet had an effect on weight gain in female mice
*Cyp2b-null plays a role in fat metabolism