mehul patel 2016
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MEHUL PATEL13 Sherman Place Lawrenceville, NJ 08648 (609) 375-7317 [email protected]
SUMMARY Knowledgeable in RNA-binding proteins and post-translation modification processes.
Excellent understanding of basic bimolecular techniques, cancer cell biology and life sciences. Strong problem solving skills, troubleshooting and reach deadlines in timely manner. Proficient in writing and strong oral communication skills. Very detail oriented, motivated, cross-functional team player and good interpersonal skills.
EDUCATION Medical University of South Carolina Graduate School, Charleston, SC Masters of Biomedical Sciences (Aug. 2015) Major: Biomedical Sciences
Rider University, Lawrenceville, New Jersey Bachelor of Science (Dec. 2010) Major: Biochemistry Minor: General Business
SKILLS Instruments: Spectronic 20D spectrophotometer FTIR spectrometer
SpectraMax MZe HPLC- Affinity, Ion exchange & SEC NanoDrop Biocore- Surface plasmon resonance DLS
Techniques: Cell culture Western Blot: cDNA library Microscopy Protein isolation/purification PCR/qRT-PCR DNA/RNA isolation/purification Bradford dye assay RNA-IP Gel electrophoresis SDS-PAGE TFF
Cloning ELISA Ultrafiltration Ultracentrifugation Polysome analysis
Computer: Dynamic Software, End Point, ProLog, ImageJ, Adobe Photoshop, MS: Word, Power Point, Excel,
PUBLICATION Yamniuk AP1,2, Ditto N1, Patel M1, Sejwal P1, Stetsko P1, Doyle ML1. Application of a kosmotrope-based solubility assay to multiple protein therapeutic classes indicates a broad use as a high-throughput screening for protein therapeutic aggregation propensity. J Pharm Sci. 2013 Aug; 102 (8): 2424-39.
GRADUATE/ UNDERGRDUATE SCHOOL RESEARCHMedical University of South Carolina, Charleston, South Carolina Department of Oral Health Sciences- Masters’ Candidate in the College of Graduate Studies Aug. 2012- Aug. 2015Graduate research in the laboratory of Dr. Viswanathan Palanisamy, focused on proteomic analysis of RNA binding protein: CELF1 and its role in post-translation modification.
Utilizing proteomic approach such as pSILAC and Hi-Seq (NSG) to determine CELF1’s associated targets. Employ in vitro models and various molecular biology techniques to investigate the role of CELF1 and function of
its associated targets in oral squamous cell carcinoma cells (OSCC). pSILAC data validation through ribosome profiling. Identified novel CELF1 target MARCKS from pSILAC was further validated in various OSCC through western
blot, RT-qPCR, RNA immunoprecipitation (RNA-IP), overexpression through cloning and in vitro transceint knockdown (shRNA) models.
Generating overexpressing MARCKS clones to further support our findings and support the overall hypothesis. Used various migration assays to understand CELF1 and MARCKS migratory role in OSCC.
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Rider University, Lawrenceville, New JerseyIndependent Research: Sept. 2009- May 2010
The objective of my project was to purify various G proteins in order to use them as reagents for identification of complexes between these proteins and other signal transduction proteins inside cells. In order to identify these complexes, I have used combination of immunoprecipitation and Western blotting techniques. The overall goal of the research was to construct an in vitro system to study G-protein Coupled Receptor signaling (GPCRs).
1st Step- Isolated rod outer segment (ROS) from bovine retinas 2nd Step- Isolated heterotrimeric G protein from membrane 3rd Step- Use affinity chromatography to separate G and G subunits
WORK EXPERIENCE
Bristol Myers Squibb, Princeton, New JerseyProtein Science & Structure - Research Associate Nov. 2011- Apr. 2012
Preparation of many biological buffer solutions to aid in high throughput stability screening of protein reagents and biologics drug candidates.
Preparation of protein samples by spin-concentration and dialysis into panels of buffers. Conducted library screening through high-throughput thermal and aggregation biophysical stability
measurements. Utilized HPLC (Size-exclusion, ion-exchange and affinity chromatography) to asses biologics for their structural
integrity and further usage in high-throughput screening method development. Knowledgeable of protein-protein interactions of biologics candidates by surface plasmon resonance (SPR) and
microcalorimetry techniques. Developed volume exclusion methods using Ammonium Sulfate and Polyethylene Glycol (PEG) for high
throughput solubility measurement of protein drug candidates. Handle multiple projects in parallel. Closely collaborate with scientists in protein biochemistry and structural
biology. Maintained accurate laboratory notebook (LIMS) and prepared written protocols and reports in GMP/GLP
compliant manner.
Johnson & Johnson, Raw Material Center, Skillman, New Jersey Supplier Verification Project- Scientist I/ Supply Chain Analyst Sept. 2010-Aug. 2011
Enabled Supplier Verification Project in an accordance with new FDA regulations to acquire chain of custody database.
Cooperated with 100+ suppliers regarding to raw materials manufacturer data. Managed an on-online portal page and endowed with unique controlled access to each supplier & distributor to
submit Requests for Information (RFIs). Hold web meetings & training with suppliers to introduce the scope of the project and the interface of the system Implement an IT database to reconcile the information received from suppliers in the most efficient and consistent
manner. Played key role in 2011 North America planning and upgrade data-mining analysis.
Tyger Scientific Inc., Ewing, New Jersey Internship May 2008- Jan 2009
Classify and input chemicals into CHEMsw to ensure inventory is accurate and up to date. Compiled marketing materials for client presentations. Utilized educational background to create lab reports to be presented to senior management. Wrote research paper on Castro Plant and determine the feasibility of new compounds.