meccanismi della modulazione immunologica dei...
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Meccanismi della modulazione immunitaria
IMMAGIE
IMMAGIE
Nelle diapositive seguenti: - I probiotici stimolano il sistema immunitario?
- Le componenti della cellula batterica coinvolte nella stimolazione
- Le sostanze secrete immunostimolanti
Probio,csandtheimmunesystem
Basicfacts Thebeginning
• Modula'onoftheimmune
system(GALTbutnotonly…)iswellestablished
• Modula,onisstrain
dependent(some,mesoppositeac,onswithinthesamespecies)
• Thefirststeps:a)miceimmunized
intraperitoneally(Bloksmaetal,1979)b)humanorallyfedduringaSalmonellavaccinetreatment(Schiffrinetal,1995)
• Twosecondsteps:a)supportvaccina,onb)deliveryvectors(GMM)
• Speciesandstrainsused:• L.caseiNCCNCC2461andL.paracaseiDN-114001forelderly• L-rhamnosusATCC53103andL.fermentumCECT5716foradults
• Dailydosagefrom1billionto10billions• Treatmentfrom28daysupto13weeks
Adjuvantsinfluvaccineinbrief
Adjuvanteffectsincombina,onwithvaccineconsis,ngofaZenuatedviruses.
Virus:fluvirusesH1N1,H3N2andBinadultsandelderly;Hepa,,sininfants
Adjuvantsinfluvaccineinbrief
• About400elderlyand50adultsinthetreatmentgroups;
• 4differentlactobacilliused,allbelongingtotheheterofermenta,vetaxonomicgroups
• Differentoutcomes;alltrialsoverallposi,vebut(apparently)withadifferentac,vityagainstdifferentviralstrains
• Strainspecificityordifferentprotocoldesign?
• Specifican,bodyresponsestoinfantHepa,,sBvaccina,on(Sohetal,2010);twostrains
(B.longumBL999andL.rhamnosusLPR)Posi,veoutcomesinthecaseofasingledose,notinascheduleof3doses
Adjuvantsinothervaccinacina,on
• Inac,vatedcholerainchildren:onestudyinBangladeshwithB.breveBBG-01(Matsudaet,2011):noadjuvanteffectinchildren
• Inac,vatedcholerainadults:6outof7strainsassayedsingularlyshowedfasterimmuneresponsemeasuredwithbymeansofserumimmunoglobulinbutoverallvaccina,onwasnotinfluenced.(Paineauetal,2008)
Adjuvantsinothervaccinacina,on
• L.acidophilusLAVRI-A1atadailydosageof3X109CFU/dayx6months(Tayloretal,2006).Tetanus(TT)andStaphylococcus
• enterotoxinB(SEB)vaccinesused.• “Insummary,althoughwedidnotseeanyconsistenteffectsonallergen-specificresponses,ourstudysuggeststhatprobio,csmayhaveimmunomodulatoryeffectsonvaccineresponses.”
Adjuvantsinothervaccinacina,on
• Theeffectsofprobio,csonan,bodyresponsestodiphtheria,tetanusandHaemophilusinfluenzaevaccinesin6-month-oldinfantsweremesuredbyKukkonenetalin2006.
• Probio,csusedwerefourstrains(LGG,L.rhamnosusLC705,B.breveBbi99andP.freudenreichii
ssp.shermaniiJS)• Differencesinconcentra,onsofan,-diphtheriaoran,-
tetanusIgGwerenotsignificant.Intheprobio,cgroup,thegeometricmeanHibIgGconcentra,ontendedtobehigher,butthedifferencewasnotsignificant
Adjuvantsinothervaccinacina,on
The probiotic mixture was formed by 4 strains; The clear final effect was vaccine-related
Adjuvantsinothervaccina,on
Meccanismi della modulazione immunitaria
IMMAGIE
IMMAGIE
Si, con una azione ceppo specifica e attraverso due categorie di meccanismi: 1. Interazione fra GALT e strutture di superficie dei batteri 2. Interazione con sostanze secrete e assorbibili