LOGOLOGOLOGOLOGO

Sequence analysis of protein Sequence analysis of protein Sequence analysis of protein Sequence analysis of protein MB21D1MB21D1MB21D1MB21D1

组别：组别：组别：组别：G5G5G5G5组员：杨永康组员：杨永康组员：杨永康组员：杨永康 高居一高居一高居一高居一 王帅王帅王帅王帅 鲁慧囡鲁慧囡鲁慧囡鲁慧囡

报告人报告人报告人报告人：：：：高居一高居一高居一高居一

2013-2013-2013-2013-1111----11111111

STINGSTINGSTINGSTING

Innate immune recognition of DNA

AminoAminoAminoAmino acid sequence of mouse acid sequence of mouse acid sequence of mouse acid sequence of mouse MB21D1MB21D1MB21D1MB21D1 protein (E330016A19). protein (E330016A19). protein (E330016A19). protein (E330016A19). Peptides identified by massPeptides identified by massPeptides identified by massPeptides identified by mass spectrometry are shown in spectrometry are shown in spectrometry are shown in spectrometry are shown in red.red.red.red.

Number of amino acids: 522Molecular weight: 58814.4Theoretical pI: 9.54

MB21D1 belongs to MB21D1 belongs to MB21D1 belongs to MB21D1 belongs to Mab-21Mab-21Mab-21Mab-21 family family family family

C-terminal of MB21D1 is not C-terminal of MB21D1 is not C-terminal of MB21D1 is not C-terminal of MB21D1 is not that conserved as its Mab-21 that conserved as its Mab-21 that conserved as its Mab-21 that conserved as its Mab-21 domain among speices.domain among speices.domain among speices.domain among speices.

Homology of MB21D1 Homology of MB21D1 Homology of MB21D1 Homology of MB21D1 matches the matches the matches the matches the evolutional evolutional evolutional evolutional relationship among relationship among relationship among relationship among speices overall.speices overall.speices overall.speices overall.

MB21D1 may function as a MB21D1 may function as a MB21D1 may function as a MB21D1 may function as a nucleotidyltransferasenucleotidyltransferasenucleotidyltransferasenucleotidyltransferase

NNNNucleotidyltransferase (NTase) family ucleotidyltransferase (NTase) family ucleotidyltransferase (NTase) family ucleotidyltransferase (NTase) family iiiincludes adenylate cyclase, poly[A] ncludes adenylate cyclase, poly[A] ncludes adenylate cyclase, poly[A] ncludes adenylate cyclase, poly[A] polymerase and DNA polymerasespolymerase and DNA polymerasespolymerase and DNA polymerasespolymerase and DNA polymerases，，，，and is relative to Mab-21 family.and is relative to Mab-21 family.and is relative to Mab-21 family.and is relative to Mab-21 family.

NTP_transf_2NTP_transf_2NTP_transf_2NTP_transf_2

Adenyl_cycl_NAdenyl_cycl_NAdenyl_cycl_NAdenyl_cycl_N

PolyA_polPolyA_polPolyA_polPolyA_pol

This superfamily has a This superfamily has a This superfamily has a This superfamily has a G[G/S]xG[G/S]xG[G/S]xG[G/S]x9-139-139-139-13[E/D]h[E/D]h [E/D]h[E/D]h [E/D]h[E/D]h [E/D]h[E/D]h motif.motif.motif.motif.

>Q3C6L5_MOUSE>Q3C6L5_MOUSE>Q3C6L5_MOUSE>Q3C6L5_MOUSE>M21D1_HUMAN>M21D1_HUMAN>M21D1_HUMAN>M21D1_HUMAN>Motif1>Motif1>Motif1>Motif1GSGSGSGSBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBDDDDBBBBDDDDBBBB>Motif2>Motif2>Motif2>Motif2GSGSGSGSJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJDDDDJJJJDDDDJJJJ

MB21D1_MOUSEMB21D1_MOUSEMB21D1_MOUSEMB21D1_MOUSE RLLRRMQKRESEFKGVEQLNTGSGSGSGSYYEHVKISAPNEEEEFDDDDVMFKLEVPRIELQEYYETGAFYHHHHHHHHHHHHHHHHHH EEEEE HHHHHHHHHHHHHHHHHH HHHHMB21D1_HUMANMB21D1_HUMANMB21D1_HUMANMB21D1_HUMAN DHLLLRLKCDSAFRGVGLLNTGSGSGSGSYYEHVKISAPNEEEEFDDDDVMFKLEVPRIQLEEYSNTRAYYHHHHHHH EEEE EE EEEEEE HHHHHHHHHHHHHHHHH HHH OAS1_HUMANOAS1_HUMANOAS1_HUMANOAS1_HUMAN LKERCFRGSSYPVCVSKVVKGGSGSGSGSSGKGTTLRGRSDDDDADDDDLVVFLSPLTTFQDQLNRRGEFIH EEEEEEEE EEEE EEEEEEE EE H

Predicted secondary structure is indicated abovePredicted secondary structure is indicated abovePredicted secondary structure is indicated abovePredicted secondary structure is indicated above,,,, alpha helices (H) and beta strands alpha helices (H) and beta strands alpha helices (H) and beta strands alpha helices (H) and beta strands(E)(E)(E)(E)

MB21D1 MB21D1 MB21D1 MB21D1 exhibitexhibitexhibitexhibitssss structural and sequence homology to the structural and sequence homology to the structural and sequence homology to the structural and sequence homology to the catalytic domain of oligoadenylate synthase (OAS1)catalytic domain of oligoadenylate synthase (OAS1)catalytic domain of oligoadenylate synthase (OAS1)catalytic domain of oligoadenylate synthase (OAS1)

LOGOLOGOLOGOLOGO

THX^^