mass spectrometry proteomics and...
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Research Center for Civilization Diseases
LIFE – Research Center for Civilization DiseasesLeipzig University Philipp-Rosenthal-Str. 2704103 [email protected]
contact person: Prof. Dr. Uta Ceglarek [email protected]
Mass SpectrometryProteomics and Metabolomics
In the LIFE Research Center
LIFE is funded by means of the European Union, by the European Regional Development (ERDF) an by means of the Free State of Saxony withhin the framework of the excellence initiative.
Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics University Hospital Leipzig AöR Liebigstr. 2704103 Leipzig
director: Prof. Dr. Joachim Thiery www.ilm.uniklinikum-leipzig.de
• Preanalytical Investigation of Polyunsaturated Fatty Acids and Eicosanoids in Human Plasma by Liquid Chromatography-Tandem Mass Spectrometry; Dorow et al.; Biopreserv Biobank; 2016; 14(2); 107-113.
• Immunoassay or LC-MS/MS for the measurement of salivary cortisol in children? Bae, Gaudl et al.; Clin Chem Lab Med; 2016; 54(5); 811-822.
• LC-MS/MS-based quantification of cholesterol and related metabolites in dried blood for the screening of inborn errorsof sterol metabolism; Becker et al.; Anal Bioanal Chem; 2015; 407(17); 5227-5233.
• Preanalytical standardization of sphingosine-1-phosphate, sphinganine-1-phosphate and sphingosine ana-lysis in humanplasma by liquid chromatography-tandem mass spectrometry; Ceglarek et al.; Clin Chim Acta; 2014; 435; 1-6.
• Preanalytical standardization for reactive oxygen species derived oxysterol analysis in human plasma by liquid chromatography-tandem mass spectrometry; Helmschrodt et al.; Biochem Biophys Res Commun; 2014; 446(3); 726-730.
• Quantification of seven apolipoproteins in human plasma by proteotypic peptides using fast LC-MS/MS;Ceglarek, Dittrich et al.; Proteomics Clin Appl; 2013; 11-12; 794-801.
• Fast LC-MS/MS analysis of free oxysterols derived from reactive oxygen species in human plasma and carotid plaque;Helmschrodt et al.; Clin Chim Acta; 2013; 425; 3-8.
• Fast liquid chromatography-quadrupole linear ion trap-mass spectrometry analysis of polyunsaturated fatty acids andeicosanoids in human plasma; Kortz et al.; J Chromatogr B Analyt Technol Biomed Life Sci; 2013; 927; 209-13.
Services Metabolomics and proteomics in the LIFE research program
The coupling of liquid chromatography and mass spectrometry enables rapid and highly sensitive analysis of biologically active metabolites, peptides, and proteins in body fluids. Within the LIFE research program a mass spectrometric platform for metabolom and proteom analysis has been established, allowing targeted and simultaneous quantitation of metabolites and proteins. By means of ultra-high per-formance liquid chromatography (UHPLC) in combination with quadrupol linear ion trap mass spectrometry, it is possible to detect and quantitate metabolites in con-centration ranges of ng/L.
In the past years method development focused on metabolic pathways that are related to vascular and cardiovascular di-seases such as arteriosclerosis or cardiac insufficiency, as well as diabetes and de-mentia. Standardized preanalytical proto-cols covering sample drawing, handling, and sample preparation were developed for ensuring reliable quantitation of partly very low abundant metabolites and pro-teins in body fluids. Methods developed in the mass spectrometry workgroup were validated for the analysis in body fluids and were partly accredited according DIN EN ISO 15189:2014 and 17025:2005.
Standardized Preanalytical Protocols
State of the Art mass Spectrometry
Quality ensured Quantitative Analysis according FDA and CLSI
Standardized Data Processing
In the LIFE research program, samples from patient popula-tions are used to create biomarker profiles paving the way for identifying novel candidate genes for civilization diseases in the line of genome-wide association studies (GWAS). Aim of method development is their potential future application in the clinical routine laboratory. Since the beginning of the year 2016, the simultaneous quantitation of nine steroid hormo-nes via liquid chromatography tandem mass spectrometry is pioneeringly and invariably applied to routine diagnostic ana-lysis at the Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics of University Hospital Leipzig AöR.
Analyte classesSteroid hormones*Apolipoproteins*Amino Acids/AcylcarnitinesSterol Lipids*Cholesterol EstersEicosanoids/ResolvinsPoly-Unsaturated Fatty Acids Sphingolipids*OxysterolsBile AcidsPhospholipidesEndocannabinoidsTriglycerides
*accredited according DIN EN ISO 15189:2014 and DIN EN ISO 17025:2005