marine pelagic protists revealed by metabarcoding · 2019-02-15 · marine pelagic protists...
TRANSCRIPT
Marine pelagic protistsrevealed by metabarcoding
Karoline Saubrekka
SupervisorsProfessor Bente EdvardsenDoctor Anders K. Krabberød
• Eukaryotes
Protists
From Caron et al. 2012 based on Baldauf 2008.
Marine pelagicprotists
0.2 – 200 µmPhytoplankton
Marine environmental monitoring
• Cornerstone of the marine foodweb• Predators, decomposers, parasites• Climate change• Eutrophication• Toxic species• Invasive species• Good taxonomer• Large (>15 µm)• Well preserved
MetabarcodingCollect water sample
• 2 year periode• 5 m
Collect water sample
Filtration
> 0.22 µm
Collect water sample
Filtration
DNA extraction
Amplify markerregion
Genetic marker: V4 region in the 18S ribosomal gene
Collect water sample
Filtration
DNA extraction
Amplify barcoderegion Sequencing
Sequence filtering
Clustering (OTU)
Taxonomic classification
DiversityCommunity stucture
Population dynamics
What groups of protists can be detected by metabarcoding in the inner Oslofjord?
How did the samples vary through thesampling period?
Revealed only by metabacoding: • Parasites• Naked celles
Revealed only by light microscope: • Lack interspesific variation in the marker• Lack sequences in the referance database
Light microscope cell count data by NIVA (Norli & Eikrem, 2018)Metabarcoding data
Light microscope vs. Metabarcoding?Light microscope + Metabarcoding
Thank you!