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MARINE ENVIRONMENT PROTECTION COMMITTEE 75th session Agenda item 4

MEPC 75/INF.21 24 January 2020 ENGLISH ONLY

Pre-session public release: ☒

HARMFUL AQUATIC ORGANISMS IN BALLAST WATER

Information on the type approval of Hyde GUARDIAN-US

Ballast Water Treatment System

Submitted by Norway

SUMMARY

Executive summary: This document provides information to the Organization by the Norwegian Maritime Authority that it has type approved the Hyde GUARDIAN-US Ballast Water Treatment System (BWTS) manufactured by Calgon Carbon UV Technologies LLC, d/b/a Hyde Marine in accordance with the Code for Approval of Ballast Water Management Systems (BWMS Code) in compliance with regulation D-3.1 of the International Convention for the Control and Management of Ships' Ballast Water and Sediments, 2004

Strategic direction, if applicable:

2

Output: 2.2

Action to be taken: Paragraph 40

Related documents: Resolutions MEPC.279(70) and MEPC.300(72)

Introduction 1 The BWMS Code, adopted by resolution MEPC.300(72), entered into force on 13 October 2019 revoking the 2016 Guidelines (G8). The 2016 Guidelines (G8) were the applicable guidelines when testing of this BWMS was planned and performed. The BWMS Code has thereafter been used for the certification. 2 The Norwegian Maritime Authority hereby notifies the Organization of its decision to type approve the Hyde GUARDIAN-US Ballast Water Treatment System (BWTS) manufactured by Calgon Carbon UV Technologies LLC, d/b/a Hyde Marine.

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3 DNV GL, on behalf of the Norwegian Maritime Authority, has reviewed technical documentation, test plans and reports throughout the type approval process. Surveys of the test facilities and the ship used for testing were also performed by DNV GL on behalf of the Norwegian Maritime Authority. 4 The Norwegian Maritime Authority finds that the system satisfies the requirements of the BWMS Code adopted by resolution MEPC.300(72). 5 This document is prepared in accordance with part 7 of the annex to resolution MEPC.300(72), Type approval report. The following information for the type approval of the Hyde GUARDIAN-US BWTS is provided:

.1 approval date: 23 January 2020; .2 name of the Administration: Norwegian Maritime Authority; .3 name of the manufacturer: Calgon Carbon UV Technologies LLC, d/b/a Hyde

Marine; .4 trade name and product designation: Hyde GUARDIAN-US BWTS; .5 a copy of the Type Approval Certificate can be found in annex 1 to this

document;

.6 summary results* from land-based testing and shipboard testing have been included in annexes 2 and 3 of this document.

Executive summary 6 The Hyde GUARDIAN-US BWTS has been successfully tested in accordance with resolution MEPC.300(72) from July 2018 to October 2019. The type approval testing has been conducted by the test facility DHI Denmark for land-based and shipboard biological efficacy testing and by Retlif Testing Laboratories for environmental testing. 7 The type approval of the Hyde GUARDIAN-US BWTS includes product series with flow rates (treatment rated capacity) ranging from 60 to 3000 m3/h. 8 The system successfully met the ballast water performance standard (D-2) in at least five consecutive land-based test cycles each in the three defined salinity ranges (marine, brackish and fresh water). The system also met the D-2 standard in at least two test cycles each in the three defined salinity ranges with a holding time of 24 hours. Furthermore, the system successfully demonstrated compliance with the performance standard (D-2) after 5 days holding time with no signs of regrowth in at least two test cycles in each of the three defined salinity ranges. Land-based testing challenge water parameters were selected to validate the System Design Limitations (SDL) for the Hyde GUARDIAN-US BWTS, and the system demonstrated compliance when operating within the boundaries of these SDLs.

* Owing to the volume of these test reports, only executive summaries are included in the annex to this INF

document. The complete test reports from land-based and shipboard testing, together with reports from environmental testing, can be obtained by making an email request to [email protected]. Requests for further information on the type approval of the BWTS may also be made to the same email address.

mailto:[email protected]

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9 Shipboard testing was conducted on board the ship Bore Sea (IMO 9443554) in harbours of the Baltic Sea such as Lübeck, Gdynia, Hanko and St. Petersburg during its normal trade route. Five consecutive, valid and successful test cycles complied with the requirements for density of viable organisms during uptake as defined in resolution MEPC.300(72), and the D-2 standard during discharge. 10 All electrical and electronic components of the Hyde GUARDIAN-US BWTS have successfully completed environmental testing in accordance with the test requirements specified by IACS UR E10 (rev.6). Description of the BWMS 11 The Hyde GUARDIAN-US BWTS utilizes filtration and UV disinfection to treat ballast water at uptake. During discharge, the ballast water is treated again by UV disinfection. The BWMS monitors the UV intensity, ballast water flow and controls the ballast flow rate to meet a target UV dose for the treatment. 12 The main components of the Hyde GUARDIAN-US BWTS are an automatic self-cleaning filter with a 20 μm mesh screen manufactured by Filtrex and a UV reactor manufactured by Hyde Marine with medium pressure UV lamps. Different model sizes of the filter and UV reactor can be installed separately and can be combined forming in total 19 different models of the Hyde GUARDIAN-US BWTS. Nine of these models are configured as Ex-proof models (with suffix "X"). The Ex-proof version of the BWTS is suitable for installation in hazardous area zone 1, but the electrical supply and control systems are to be located in a safe area zone. More technical details can be found in annex 1 to this document. 13 The Hyde GUARDIAN-US BWTS has demonstrated performance to the D-2 discharge standard at a UV dose above 170 mJ/cm2 for all water qualities. If the UV dose is monitored to be below 170 mJ/cm2, the system automatically reduces the flow rate. Minimum flow corresponds to the limitation of the control valve (V4) which can regulate the flow until it is 15% open. The system has demonstrated performance to the D-2 discharge standard at a flow rate down to 17% of TRC at a UV Intensity measured at 133 mW/cm2 in water with a UVT of 50% in brackish water and at a flow rate down to 30% of TRC at a UV Intensity measured at 209 mW/cm2 in water with an UVT of 48% in fresh water. The alarm set point for the UV dose is 170 mJ/cm2 for all salinities. 14 Hyde GUARDIAN-US models HG300U BWTS and HG750U BWTS have been tested during this type approval. 15 Hyde Marine also offers the GUARDIAN Gold BWTS in the market. The difference between the GUARDIAN "Gold" BWTS and the GUARDIAN "US" BWTS (Universal Service) is related to filter mesh, a higher target UV dose (including stronger UV lamps) as well as a software upgrade. In many other respects, most of the components of the system for "Gold" and "US" are very similar. Compared to GUARDIAN Gold BWTS, the Hyde GUARDIAN-US BWTS was tested to achieve ballast water treatment with a shorter holding time and at a UV dose mainly designed for USCG type approval (live organisms ≥10 and <50 μm were quantified by microscopy counting after staining with CMFDA/FDA). Overview of the type approval process 16 Based on review of documentation as required in paragraphs 1.3 and 1.4 in part 1 of the annex to MEPC.300(72), inspections of the system's control and monitoring system during functional tests, inspections of the installations at the test sites and on board the ship, and witnessing of commissioning test cycles, DNV GL qualified the Hyde GUARDIAN-US BWTS

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design to be in accordance with part 1 and part 5 of the annex to resolution MEPC.300(72). The test set-up was verified to be in accordance with paragraphs 1.5 to 1.15 in part 1 of the annex to resolution MEPC.300(72). 17 All land-based and shipboard test cycles were performed independently by the test facility DHI in Denmark. Environmental testing was performed by Retlif Testing Laboratories in the USA. The test facilities provided test plans and test reports. 18 DNV GL reviewed and approved the test plans and test reports provided by the test facilities and found them to be in compliance with resolution MEPC.300(72). Surveys were performed by DNV GL during biological efficacy test cycles at the land-based test facility and on board the ship used for testing. 19 DHI Denmark is certified and audited by an independent accreditation body (DANAK). The Quality Management Plan (QMP), Quality Assurance Project Plan (QAPP), and test plans for the Hyde GUARDIAN-US BWTS testing prepared by DHI have been reviewed by DNV GL on behalf of the Norwegian Maritime Authority and found in compliance with resolution MEPC.300(72). 20 Any augmentation of test water has been validated by DHI in accordance with paragraph 2.4.21 and all methods for sampling and analysis were in accordance with part 4 of the annex to resolution MEPC.300(72) and to the satisfaction of the Administration. During land-based testing, the BWTS has been challenged with test water with a low UVT of 48-61% in at least two test cycles per salinity, which represents a UVT at the lower end of the UVT in ambient water that would quantitatively meet the stated water quality criteria for fresh and brackish water, and a much lower UVT than what is common for marine water. A mix of Standard Test Organisms and ambient organisms in brackish and marine water was employed at DHI to ensure robust testing. For fresh water test cycles, challenge water contained ambient organisms only. Methods used for enumeration of the ≥10 and <50 μm size organisms were FDA/CMFDA. 21 Land-based testing of the GUARDIAN-US HG300U BWTS, equipped with a Filtrex ACB-945-200 filter with 20 μm mesh and the UV reactor model G300684 was conducted at DHI from July to October 2018. Fifteen valid successful land-based test cycles were performed in the three defined salinity ranges for the GUARDIAN-US HG300U model. The system successfully met the D-2 standard in at least five consecutive test cycles in each of the three defined salinity ranges (marine, brackish and fresh water). More details of the land-based test cycles are given in annex 2 to this document. 22 Shipboard testing of GUARDIAN-US HG750U BWTS, equipped with Filtrex ACB-985-300 filter with 20 μm mesh and the UV reactor model G300815, was conducted on board the ro-ro ship Bore Sea (IMO 9443554, Netherlands flag) from November 2018 to October 2019. The BWTS was operated independently by the ship's crew during testing in ports in the Baltic Sea (Lübeck, Gdynia, Hanko and St. Petersburg) during its normal trade routes. A total of 11 test cycles were conducted with five consecutive valid successful test cycles. More details of the shipboard test cycles are given in table 4 and in annex 3 to this document. 23 DNV GL has evaluated all electrical and electronic components of the Hyde GUARDIAN-US BWTS for relevant required environmental tests. Relevant components were identified for environmental testing at the test facility Retlif in the USA, which is approved as a laboratory to DNV GL. All tests performed were done according to the pre-approved test specification developed by Retlif in accordance with IACS UR E10 as specified by resolution MEPC.300(72). Environmental testing included, as relevant, vibration, cold and

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heat, damp heat, power variation, inclination and EMC tests. Some instruments are common marine equipment and were already certified under classification society rules in accordance with IACS UR E10 (rev.6). Operational safety requirements 24 The BWMS has two layers of safety to prevent overheating and over pressure inside the UV reactor. The temperature and water level in the reactor are monitored and the BWMS will also shut down in case of low flow rate. If failure occurs on the flow meter, the temperature sensor will serve as a second barrier for safety, and the system will shut down if the temperature exceeds the design set point. If a failure occurs to the control system, the BWTS has an independent temperature switch to shut down the lamp power. 25 Further, the system will also shut down if the system measures a high differential pressure across the filter (>0.5 bar). 26 The Hyde GUARDIAN-US BWTS design is verified to be in accordance with DNV GL Rules for Classification of Ships. Discussion 27 Resolution MEPC.279(70) and the US Coast Guard regulations for type approval testing were the relevant guidelines at the time of type approval testing of the GUARDIAN-US BWTS at DHI. However, the test specifications and challenge water characteristics of resolution MEPC.279(70) are identical with resolution MEPC.300(72). 28 In accordance with paragraph 6.6 in part 6 of resolution MEPC.300(72), table 1 presents the validation of the manufacturer’s stated System Design Limitations (SDL) and the corresponding tested ranges in valid and successful land-based testing. Table 2 lists other operational parameters relevant for the operation of the Hyde GUARDIAN-US BWTS and the ranges tested in land-based and shipboard testing. Table 1: Summary of System Design Limitations (SDL) of the Hyde GUARDIAN-US BWTS and overview of range tested in land-based testing of the HG300U BWTS, including challenge water parameters selected to validate the SDLs (based on the test report in annex 2)

Parameter Unit Vendor’s claim

System Design Limitation Range in valid and

successful test cycles

Treatment rated capacity (TRC)

(1) m3/h 300

52 - 235 (ballast) 53 - 246 (deballast)

Minimum UV dose mJ/cm2 170 178 - 229 (ballast)

175 - 185 (deballast)

Low flow UVT limit % 49 48

Full flow UVT limit % 77 -

Salinity PSU no limitation 0.33 - 30

Temperature of ballast water °C 0 - 40 12 - 24

Holding time hours not applicable 24 - 120

Power consumption kWh/100m3 19.2 25 - 115

(1) Average flow rate measured after filtration during ballasting

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Table 2: Operational restrictions for Hyde GUARDIAN-US BWTS and tested ranges during land-based (LB) testing of the HG300U BWTS, and during shipboard (SB) testing of the HG750U BWTS


System Design Limitation Range in valid and


UV dose set point for reducing lamp power to 60% (power level 1)

mJ/cm2 >295 -

Filter differential pressure for initiating the backflushing cycle

Δbar >0.3 0.3

Filter inlet pressure bar >1 1.5 - 2.7 (LB testing) 1.8 - 3.3 (SB testing)

Filter differential pressure at which the system will shut down

Δbar >0.5 tested using simulated signal

Maximum operation pressure bar 10 not tested

Power per UV lamp kW 6.0 7.7

3.6 and 6 (LB) 4.6 and 7.7 (SB)

29 Table 3 presents the challenge water used during land-based testing. Since the system applies flow regulation, test water prepared for the land-based testing in some occasions exceeded the minimum challenge water requirements defined in part 2 of the annex to resolution MEPC.300(72), in order to further challenge the system limitations for minimum UV intensity. Table 3: Minimum challenge water requirements for land-based testing and tested ranges during land-based testing of the Hyde GUARDIAN-US BWTS (based on test report in annex 2)

IMO requirements on challenge water parameter Range in valid and


Parameter Unit Value for 0-20 PSU

Value for 28-36 PSU

0.33 - 30 PSU

DOC mg/L >5 >1 6.5 - 15

POC mg/L >5 >1 5.8 - 8.3

TSS mg/L >50 >1 44 - 81

Organisms ≥50 µm

org/m3 ≥105 116,000 - 1,795,000

diversity 5 species from 3 phyla/divisions fulfilled

Organisms ≥10-<50 µm (1)

org/mL ≥103 1,050 - 3,590

diversity 5 species from 3 phyla/divisions fulfilled

Heterotrophic bacteria CFU/mL ≥104 15,000 – 59,000

(1) Live organisms ≥10 and <50 μm were quantified by microscopy counting after staining with CMFDA/FDA.

30 Despite the best efforts of the test facility staff to prepare test water meeting all relevant challenge water requirements, there were some deviations from the minimum criteria. During tests B-4, B-5 and B6 the flow rate of discharge of the control water was higher than

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that of the treated water. This was considered conservative, since higher flow rates from the control water would lead to higher die-off in the control water. Brackish water tests B-3 and B-6 did not pass the D-2 standard since the control logic of valve V4 prevented the BWMS to deliver the full target dose. The tests were deemed invalid due to this error after a technical evaluation and software corrections were done after each of these tests. 31 The BWMS has demonstrated performance to the discharge standard at the target dose of 170 mJ/cm2 applied during both the ballasting and deballasting operation. Under challenging conditions, the BWMS uses flow reduction to achieve the minimum dose. This explains why most of the test cycles documented under this type approval do not reach the maximum flow rate (TRC) since extreme conditions during testing have been planned for. Performance at near Treatment Rated Capacity (full flow) was demonstrated at a UVT of about 86% (SB-2). With flow reduction, the BWMS successfully performed with challenge conditions reflecting a UVT of 48% (F-2). This corresponds to challenge water that quantitatively and qualitatively meets the minimum water quality criteria of resolution MEPC.300(72). 32 A total of 11 shipboard test cycles were conducted on board the ship Bore Sea with the GUARDIAN-US HG750U BWTS model. Shipboard test cycles SB-1 and SB-3 were invalid due to insufficient counts of organisms of which SB-1 complied with the discharge standard, while SB-3 analysis was abandoned. Test cycles SB-5, SB-6, SB-8 and SB-10 were each determined invalid due to technical and procedural reasons. Each of these test cycles were thoroughly evaluated, both by Hyde Marine and DNV GL and the reasons found included filter damage, tank contamination due to specific piping arrangements and improper operation of the BWTS which was not according to the OMSM. 33 Shipboard testing was carried out at a flow rate of between 381 and 682 m3/h which was of a capacity that reflects ballast water pump capacity on board the ship used and the full rated capacity of the model HG750U BWTS as required by paragraph 2.8. in part 2 of the annex to resolution MEPC.300(72). 34 All log data from the control and monitoring system have been saved and collected after the last shipboard test. These data have been evaluated to confirm that the Hyde GUARDIAN-US HG750U BWTS was working as intended. The ballast water record book of the ship confirms that the BWTS was used throughout the testing period as required by paragraph 2.6.11 in part 2 of the annex to resolution MEPC.300(72). The actual test period was about 11 months and thus more than the required 6 months. 35 A summary of the shipboard test cycles including the SDLs and corresponding tested ranges is given in table 4 below.

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Table 4: Summary of System Design Limitations (SDL) of the Hyde GUARDIAN-US BWTS and overview of range tested in shipboard testing of the HG750U BWTS equipped with a filter Filtrex ACB -985-300 filter with 20 μm mesh and the UV reactor model G300815, including water parameters (based on the test report in annex 3)


System Design Limitation

Range tested in valid and successful test

cycles

Treatment rated capacity (TRC) (1)

m3/h 750 381 - 682

Minimum UV dose mJ/cm2 170 199 - 436 (ballast)

182 - 259 (deballast)

Salinity PSU No limitation 0.18 - 15

Temperature of ballast water °C 0 - 40 4.3 - 22

Holding time hours Not applicable 20 - 37

Power consumption kWh/100m3 18.4 -

IMO requirements on challenge water parameter

Parameter Unit Value Range in valid and


Organisms ≥50 μm org/m3 >100 at uptake 9.900 – 40,000

Organisms ≥10-<50 μm org/mL >100 at uptake 120 - 760

(1) Average flow rate measured after filtration during ballasting.

36 To fulfil the requirements to evaluate effective performance in a range of ballast water temperature as specified in section 2.46 in part 2 of the annex to resolution MEPC.300(72), testing of the Hyde GUARDIAN-US BWTS was performed in temperatures ranging from 4.3 to 24°C. The Hyde GUARDIAN-US BWTS was found to have no limitations with regards to the temperature of the ballast water to be treated. Performance of the Hyde GUARDIAN-US BWTS at temperatures higher than 24°C and up to 40°C is evaluated to have no influence on the UV dose given by UV emission spectrum in a temperature range between 0 and 40°C. 37 The Hyde GUARDIAN-US BWTS has demonstrated performance to the discharge standard with a holding time between uptake and discharge of 24 hours in land-based testing in at least two test cycles in each of the three defined salinity ranges as required by paragraph 2.13 in part 2 of the annex to resolution MEPC.300(72). UV treatment is instant and does not require any holding time in a ballast tank. Therefore, holding time is not found to be a limiting condition for the ballast water management system. 38 The requirement to evaluate regrowth as specified in paragraph 2.49 of part 2 of the annex to resolution MEPC.300(72) have been fulfilled by full-scale valid and successful land-based testing conducted with a holding time of at least 5 days. Results show that Hyde GUARDIAN-US BWTS performs to the D-2 discharge standard over the full range of tested salinities (<1-32 PSU) in two test cycles in each of fresh, brackish and marine water at DHI. The results of these land-based test cycles are presented in annex 2 to this document. 39 Evaluation of scaled units as required by paragraph 4.15 of resolution MEPC.300(72) has confirmed that scaled models have equivalent performance as compared to the base model that was used in land-based testing. In accordance with BWM.2/Circ.33/Rev.1, scaled units were verified by mathematical calculation of theoretical UV-dose by the different UV reactor models, and by filter design and filtration velocity comparisons of the different filter

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models. Operational data from land-based and shipboard testing, as well as separate scaling validation tests, have been used to verify the theoretical scaling of the UV reactor and filter models. Action requested of the Committee 40 The Committee is invited to note the information contained in this document.

***

Form code: TA 251 Revision: 2016-12 www.dnvgl.com Page 1 of 10

© DNV GL 2014. DNV GL and the Horizon Graphic are trademarks of DNV GL AS.

Certificate No: TAP000020S

This is to certify:

That the Ballast Water Management System

with type designation(s) Hyde GUARDIAN-US (model range HG60U to HG3000U)

Issued to

Calgon Carbon UV Technologies LLC, d/b/a Hyde MarineCoraopolis, PA, USA

is found to comply with DNV GL rules for classification – Ships DNV GL class programme DNVGL-CP-0209 – Type approval – Ballast water management

systems IMO Resolution MEPC.300(72) - Code for Approval of Ballast Water Management Systems (BWMS Code)

Application :

This is to certify that the Ballast Water Management System listed above has been examined and tested in accordance with the requirements of the specifications contained in the BWMS Code and DNV GL Rules stated above. This Certificate is valid only for the Ballast Water Management System referred to above.

System Design Limitations / Limiting Operating Conditions imposed are described in this document.

For the compliance with the BWMS Code, the Certificate is issued on behalf of the Norwegian Maritime Authority.

Product(s) approved by this certificate is/are accepted for installation on all vessels classed

by DNV GL, unless otherwise instructed by relevant Maritime Administrations.

Issued at Høvik on 2020-01-23

This Certificate is valid until 2025-01-22.

DNV GL local station: New York

Approval Engineer: Martin Olofsson

for DNV GL

Dag Sæle-Nilsen

Head of Section

This Certificate is subject to terms and conditions overleaf. Any significant change in design or construction may render this Certificate invalid. The validity date relates to the Type Approval Certificate and not to the approval of equipment/systems installed.

MEPC 75/INF.21 Annex 1, page 1 ANNEX 1

Job Id: 262.1-033203-1 Certificate No: TAP000020S


Name of ballast water management system (BWMS)

Hyde GUARDIAN-US BWTS

Ballast water management system manufactured by

Calgon Carbon UV Technologies LLC, d/b/a Hyde Marine

Place of production

2000 McClaren Woods Drive, Coraopolis, PA 15108, USA

Type and model designations

HG60U, HG100U, HG150U, HG250U, HG300U, HG500U, HG750U, HG1000U, HG2000U, HG3000U,

HG100UX, HG150UX, HG250UX, HG300UX, HG500UX, HG750UX, HG1000UX, HG2000UX, HG3000UX

(X) implies Ex-proof configuration

Equipment / assembly drawings

The Hyde GUARDIAN-US BWTS shall be installed in accordance with the relevant documents listed below.

Description Title Drawing No. Revision

Operation, maintenance and

safety manual

Hyde GUARDIAN-US BWTS

Operations & maintenance

manual

G800099 Rev.0

2019-12-19

Bill of materials (BoM)

Hyde GUARDIAN-US System

Bill of Materials

(standard and Ex)

G800112 Rev.10

2019-12-19

Overview of drawing

packages System drawing matrix G800156

Rev.1

2019-12-19

Piping and instrumentation

diagram (P&ID) Flow schematic G500340

Rev.0

2019-10-01

Drawings of components and

arrangements

HG60U - HG1000U,

HG100UX - HG1000UX

Mechanical Package

(one for each model)

see details in

G800156 2019-12-19

Electrical wiring diagram

HG60U - HG1000U,

HG100UX - HG1000UX

Electrical Drawing Package

(one for each model)

see details in

G800156 2019-12-19

Other equipment manufactured by

The Hyde GUARDIAN-US BWTS applies the ACB self-cleaning screen filter series manufactured by Filtrex.



Treatment Rated Capacity

60 - 3000 m3/h

Product description

Treatment sequence:

• Ballast water uptake: filtration and inactivation by UV treatment

• Ballast water discharge: inactivation by UV treatment

System design limitations / Water quality parameters

Temperature & Salinity Temperature and salinity of the ballast water are not limiting conditions for the ballast water

management system.

System design limitations / Operational parameters

Holding time

Hyde GUARDIAN-US BWTS has demonstrated performance to the discharge standard with a minimum

holding time between uptake and discharge of 24 hours in land-based testing. UV treatment is instant

and does not require any hold time in a ballast tank to render organisms inviable. Therefore, holding

time is not found to be a limiting condition for the ballast water management system.

Dosing The system is designed to deliver satisfactory treatment at a UV dose greather than or equal to

170 mJ/cm2. The UV dose is a calculated value based upon flow rate and the measured UV intensity

value from the UV sensor.

A treatment alarm is given if the minimum dose cannot be maintained by the system.

The treatment starts at 100% lamp power. If the dose is above 295 mJ/cm2, the lamp power is reduced

to 60%. If the dose is below 170 mJ/cm2 the flow is modulated to maintain the minimum dose at

170 mJ/cm2. The flow can be modulated down to 15% opening of the modulating valve (V4). In practice,

this may imply flow reduction down to about 20% of the TRC in very turbid water.

Treatment Rated Capacity of the BWMS The Treatment Rated Capacities (TRC) of the designated Hyde GUARDIAN-US models are listed in the

table below. The table also specifies the filter and the UV reactor that shall be installed for a specific

model.

UV reactors and filter units can be installed in parallel configuration to achieve higher flow capacities

according to the design and installation guide and the table below.

The BWMS controls the flow rate in the ballast water line by using a flow limiting valve to ensure that the

flow rate is kept within the TRC of a specific model.



Model name TRC (m3/h) Filter unit UV unit

HG60U 60 ACB-906-100 G300808

HG100U 100 ACB-910-150 G900747D15160660

(G300811xxx160660) (1)

HG100UX 100 ACB-910-150 G300816Xyxx160660 (2)

HG150U 150 ACB-915-150 G900747D15160660

(G300811xxx160660) (1)

HG150UX 150 ACB-915-150 G300816Xyxx160660 (2)

HG250U 250 ACB-935-200 G300684D20160860

(G300811xx160860) (1)

HG250UX 250 ACB-935-200 G300816Xyxx160860 (2)

HG300U 300 ACB-945-200 G300684D20160860

(G300811xxx160860) (1)

HG300UX 300 ACB-945-200 G300816Xyxx160860 (2)

HG500U 500 ACB-955-250 G900750D25201277

(G300815xxx201277) (1)

HG500UX 500 ACB-955-250 G900753D25201277

(G300818xxx201277) (1)

HG750U 750 ACB-985-300 G900750D30201877

(G300815xxx201877) (1)

HG750UX 750 ACB-985-300 G900753D30201877

(G300818xxx201877) (1)

HG1000U 1000 ACB-999-350 G300805D40201877

(G300815xxx201877) (1)

HG1000UX 1000 ACB-999-350 G300817D40201877

(G300818xxx201877) (1)

HG2000U (3) 2000 2x

ACB-999-350

2x

G300805D40201877 (G300815xxx201877) (1)

HG2000UX (3) 2000 2x

ACB-999-350

2x

G300817D40201877 (G300818xxx201877) (1)

HG3000U (4) 3000 3x

ACB-999-350

3x

G300805D40201877 (G300815xxx201877) (1)

HG3000UX (4) 3000 3x

ACB-999-350

3x

G300817D40201877 (G300818xxx201877) (1)

(1) Alternative assembly with fabricated UV chamber (instead of cast housing)

(2) Assembly with fabricated UV chamber

(3) Capacity of 2000 m3/h is designed with 2 identical HG1000 units in parallel

(4) Capacity of 3000 m3/h is designed with 3 identical HG1000 units in parallel



Pressure

The minimum/maximum pressure and the differential pressure initiating backflushing are listed below.

Filter type Minimum inlet pressure

(back‐pressure)

Differential pressure

initiating backflushing

Maximum operating

pressure

Filtrex type ACB 1 bar ≥ 0.3 bar 10 bar

Control and monitoring equipment

Software version The Hyde GUARDIAN-US BWTS is type approved with the system control software version 04.02.02.yy.

Any changes to the software are to be recorded as long as the system is in use on board. Major changes

in the software, as defined in the Hyde GUARDIAN Software Quality Plan, document QP No.275 (Rev.0

dated 2019-11-20), require approval. Testing of the application functions of the revised software may be

required.

Safety measures

The BWMS is type approved with the following instruments for monitoring the safe operation of the

BWMS:

• Temperature sensor (TP3237, mounted in UV unit)

• Water level sensor (LMT-100, mounted in the UV unit)

• Pressure transmitter (AST4000C00010B4E1000, at the inlet and outlet of filter unit)

• Flow meter (Endress+Hausser, mounted before the UV unit)

• Temperature switch (TP3237), arranged with independent shutdown functionality (≥80°C)

Electrical and electronic components

The Hyde GUARDIAN-US BWTS is type approved with the electrical and electronic components (including

the above listed instruments for monitoring safe operation of the BWMS) indicated on the P&ID and

specified on the Bill of Material. Except for the components listed below, alternate models to the ones

specified on the component lists may be used provided that information regarding the selected

components is part of the documentation related to the specific installation, by providing either a

reference to valid type approval certificate or technical documentation demonstrating that the selected

component was subject to environmental testing as per IACS UR E10.

For the following electrical and electronic components, the models specified in the table shall be used:

Component name Manufacturer Model(s)

Control panel Hyde Marine G104605

Power supply panel Hyde Marine G103340, G103370, G103392,

G104630, G103400

UVI duty sensor (UVI reference sensor)

Jenoptik R350793

UV lamp (HG60U) Light Sources R350842

UV lamp ETA Plus R350840, R350827



Hazardous area / Ex-proof

The Hyde GUARDIAN-US BWTS has been evaluated and found to be in compliance with DNV GL Rules

Pt.4 Ch.8 Sec.11 for hazardous area installations. The filter (including indexing arm motor and pressure

transmitters), the UV reactor, the wiper drive motor assembly, valves and flowmeter have

Ex-certification and can be installed in hazardous area zone 1, gas group IIC and temperature class T4.

The power supply panel and the control panel are to be located in a safe zone. Ex-certification is not

covered by this certificate. Installation in a hazardous area are to be approved in each case according to

the Rules and Ex-certification / Special Condition for Safe Use, listed in a valid Ex-certificate issued by a

notified/recognized Certification Body.

Documents approval

The following documentation is to be submitted for each BWMS installation:

• Piping and Instrumentation Diagram (P&ID) of the ballast system including the treatment system

installation

• Power supply arrangement

• Interface description towards the ship’s existing systems including alarms for failure

• Description confirming the arrangement of alarms for bypass of the BWMS system (as part of

Ballast Water Management Plan)

• List of Ex equipment according to DNV GL Rules Pt.4 Ch.8 Sec.11 if the system is to be installed

in hazardous area zone

• Commissioning checklist

• Class survey checklist

Type Approval documentation

System descriptive documentation

• Hyde GUARDIAN-US BWTS Operations & maintenance manual, Hyde Marine, Rev.0, 2019-12-19

• G800112 Bill of Materials, Hyde Marine, Rev.10, 2019-12-19

• G800156 Hyde BWTS system drawing matrix, Hyde Marine, Rev.1, 2019-12-19

• CFD analysis for scale-up of the Hyde GUARDIAN BWMS, Hyde Marine, Rev.7, June 2019

• GUARDIAN scaling: Filter sizing, Hyde Marine, 2019-11-11

• GUARDIAN scaling: Power sizing, Hyde Marine, 2019-11-15

• Hyde GUARDIAN software quality plan, QP No. 275, Hyde Marine, Rev.0, 2019-11-20

• ISO Management System Certificate, ISO 9001:2015, issued by DNV GL 2010-11-04

• Commissioning checklist for Hyde GUARDIAN-US, Hyde Marine, Rev.1, 2019-10-15

• Class survey checklist for Hyde GUARDIAN-US, Hyde Marine, Rev.1.0, 2020-01-23

Biological efficacy test plans and test reports

• Biological efficacy performance of Hyde GUARDIAN-US in land-based test – Test Plan,

DHI, 2018-07-09.

• Performance evaluation of Hyde GUARDIAN-US in shipboard test – Test Plan, DHI, 2018-11-02

• Biological efficacy performance evaluation of Ballast Water Management System Hyde

GUARDIAN-US HG300U in land-based test – LB test report, DHI, 2019-12-03

• Biological efficacy performance evaluation of Hyde GUARDIAN-US HG750U in shipboard test,

DHI, 2019-12-18



Environmental test plan and reports

• Test procedure for ballast water management system (procedure RTP-15827-1),

Retlif, Rev.D, 2019-04-09

• Environmental test report, R-15827-2, Retlif, Rev.A, 2019-10-01

• EMC test report, R-15827-1, Retlif, Rev.A, 2019-12-04

Tests carried out

• Land-based testing using Hyde GUARDIAN-US BWTS HG300U, with a TRC of 300 m3/h with one

Filtrex ACB-945-200 filter and one G300684D20160860 UV reactor at DHI in accordance with

Resolution MEPC.300(72) and USCG 46 CFR 162.060-26

• Shipboard testing using Hyde GUARDIAN-US BWTS HG750U, with a TRC of 750 m3/h with one

Filtrex ACB-985-300 filter and one G300815D30201877 UV reactor on board the vessel Bore Sea

(IMO 9443554) in accordance with Resolution MEPC.300(72) and USCG 46 CFR 162.060-28

• Function tests of the control and automation system witnessed by DNV GL

• Environmental testing in accordance with DNVGL-CG-0339 Standard for Certification Nov. 2015

“Environmental test specification for electrical, electronic and programmable equipment and

systems”, Resolution MEPC.300(72), USCG 46 CFR 162.060-30 and IACS E10

A summary of the test results from land-based and shipboard tests are given in an annex to this

certificate.

Marking of product

For Traceability of this type approval, each treatment system is to be marked with:

• Manufacturer’s name or trade mark

• Type designation

• Serial number

Periodical assessment

For retention of the Type Approval (TA), DNV GL Surveyor shall perform periodical assessments to verify

that the conditions of the TA are not altered since the certificate was issued.

The scope of periodical assessment includes:

• Review of the TA documentation and verification that the documentation is still used as basis for

the production.

• Review of possible changes in design, material and performance of the product.

• Verification of the company’s production and quality systems ensuring continued consistent

production of the type approved products to the required quality.

• Verification that the product marking for identification and traceability to the TA Certificate is not

altered.

Copy of type approval certificate

A copy of this type approval certificate should be carried on board a vessel fitted with this Ballast Water

Management System at all times. A reference to the test protocol and a copy of the test results should

be available for inspection on board the vessel.



ANNEX 1: SUMMARY OF TESTING

Summary of land-based testing for Hyde GUARDIAN-US BWTS Table 1 Test water conditions and operational data obtained during land-based testing of the Hyde GUARDIAN-US

BWTS, model HG300U with Filtrex ACB-945-200 filter (20 µm mesh size), UV reactor (8 lamps at 6.0 kW) and

capacity TRC 300 m3/h, performed in the period July to October 2018 at DHI in Denmark. All water quality data are

inlet samples and operational data are from ballasting operations.

Test cycle

Temp. (°C)

Salinity (PSU)

UVT (1) (%)

DOC (mg/L)

POC (mg/L)

TSS (mg/L)

UVI (2) (mW/cm2)

UV dose (3) (mJ/cm2)

Flow rate average (m3/h)

Holding time

(days) before filter

after filter (4)

Fresh water test cycles

F-1 23 0.36 48 7.8 7.3 66 213 182 108 93 1

F-2 22 0.33 48 8.5 7.2 65 209 179 101 90 1

F-3 16 0.37 59 6.5 7.6 56 311 222 128 118 5

F-4 16 0.36 55 6.6 6.8 62 282 213 120 108 1

F-5 17 0.36 56 7.1 7.8 52 272 187 122 111 5

Brackish water test cycles

B-1 20 17 68 7.1 7.5 72 486 197 254 199 1

B-2 22 17 65 7.8 8.3 65 457 191 247 187 1

B-3 (5) 18 20 52 12 8.2 64 226 178 103 99 1

B-4 14 22 61 8.8 7.8 68 343 215 138 125 5

B-5 14 22 61 8.8 7.8 68 341 229 132 116 5

B-6 (5) 13 20 51 14 8.3 68 187 227 78 70 1

B-7 12 19 50 15 6.9 58 133 209 56 52 1

Marine water test cycles

M-1 24 29 50 14 7.5 81 210 180 106 92 1

M-2 18 29 73 7.2 5.8 44 508 178 232 223 1

M-3 17 29 73 7.2 5.8 44 526 181 240 235 1

M-4 13 30 61 8.3 6.2 64 340 187 151 140 5

M-5 14 30 61 8.3 6.2 64 330 190 144 137 5

(1) UVT measured during ballast operation.

(2) UVI average value during ballasting operation.

(3) UV dose average value during ballasting operation.

(4) Flow rate is calculated (as processed volume by operation time) measured after filtration during ballasting operation. (5) Test cycles B-3 and B-6 were each determined to be invalid by DNV GL due to technical issues with the BWTS that prevented the system to

deliver the target dose.



Table 2 Average densities of live organisms in inlet and treated discharge water during land-based testing of the

Hyde GUARDIAN-US BWTS, model HG300U with Filtrex ACB-945-200 filter (20 µm mesh size) and UV reactor

(8 lamps at 6.0 kW) with TRC 300 m3/h. Live organisms ≥10 and <50 µm in discharge water were were quantified

based on CMFDA/FDA. All counts of pathogenic bacteria (E. coli, Enterococci and Vibrio cholerae) in treated water

were below the ballast water discharge standard. Tests were performed in the period July to October 2018 at DHI in

Denmark.

Test cycle

Organism densities in inlet water

Organisms densities in discharge water

≥50 µm (org/m3)

≥10-<50 µm (org/mL)

≥50 µm (org/m3) ≥10-<50 µm (org/mL)

inlet inlet treated control treated control

fresh water test cycles

F-1 1,185,134 2,108 0 528,022 7.6 2,388

F-2 1,794,978 1,801 1.5 712,876 7.8 1,490

F-3 488,056 1,132 0 352,607 0 629

F-4 361,056 1,492 0 226,483 8.2 1,235

F-5 697,556 1,230 2.7 360,426 1.0 664

Brackish water test cycles

B-1 544,609 1,102 0.33 150,918 0.17 749

B-2 207,161 2,347 0 98,235 0.67 1,321

B-3 (1) 334,561 2,433 0.33 102,300 17.7 1,163

B-4 165,828 1,073 0.33 92,844 0.33 515

B-5 184,494 1,045 1.0 92,844 1.7 515

B-6 (1) 172,408 1,515 2.7 127,679 10.1 1,159

B-7 115,789 1,378 3.3 54,634 5.1 1,382

Marine water test cycles

M-1 302,878 3,590 0 83,615 1.2 354

M-2 252,883 1,310 1.0 165,626 2.7 784

M-3 271,806 1,360 0 165,626 1.8 784

M-4 224,961 1,497 0.67 72,283 0 917

M-5 211,472 1,487 0 72,283 0.17 917

(1) Test cycles B-3 and B-6 were each determined to be invalid by DNV GL due to technical issues with the BWTS that prevented the system to

deliver the target dose.



Shipboard testing

Table 3 Results from shipboard testing of Hyde GUARDIAN-US BWTS, model HG750U with Filtrex ACB-985-300

filter (20 µm mesh size) and UV reactor (18 lamps at 7.7 kW) with TRC 750 m3/h, performed in the period November

2018 to October 2019 on board the vessel Bore Sea. Test conditions: salinity: 0.18-15 PSU, temp: 4.3-22°C.

Live organisms ≥10 and <50 µm in discharge water were quantified based on FDA/CMFDA method.

All counts of pathogenic bacteria (E. coli, Enterococci and Vibrio cholerae) in treated water were below the ballast

water discharge standard.

Test cycle (1)

UVT (%)

Flow

rate (2)

(m3/h)

Holding

time (hour:min)

UVI (3)

(mW/cm2)

UV dose (mJ/cm2)

≥50 µm

(org/m3)

≥10-<50 µm

(org/mL)

inlet discharge inlet discharge

SB-2 86 682 20:18 445 208 14,821 0.91 124 4.7

SB-4 72 611 20:47 821 436 10,180 7.5 755 2.0

SB-7 80 632 34:30 523 250 12,762 9.2 242 2.5

SB-9 83 633 24:49 532 268 9,882 2.0 212 0.33

SB-11 55 381 36:59 233 199 39,638 3.0 195 2.8

(1) Test cycle SB-1 (successful) was performed in November 2018 but was declared invalid due to low phytoplankton counts. Test cycle SB-3

was abandoned due to low phytoplankton counts. Test cycles SB-5, SB-6, SB-8 and SB-10 were each determined invalid due to technical and procedural reasons, which are explained more in details in the INF report to IMO related to this type approval certificate.

(2) Average flow rate after filtration during ballast operation of treated water, measured by the BWMS flow meter located after the filter;

extracted from BWMS log.

(3) UVI average value during ballasting operation.

***

DNV GL AS Final Report

December 2019

Biological efficacy performance evaluation of Ballast Water Management System Hyde GUARDIAN-US – HG300U in land-based test

Land-based test report


DHI - BWL-DK / 11822035 / DNV-GL – Hyde Marine / Performance evaluation in land-based test facility / Final report

DHI - BWL-DK / 11822035 / DNV-GL – Hyde Marine / Performance evaluation in land-based test facility / Final report

This report has been prepared under the DHI Business Management System certified by Bureau Veritas to comply with ISO 9001 (Quality Management)

Approved by

03-12-2019

XApproved by

Signed by: Torben Madsen

DHI • Agern Allé 5 • DK-2970 Hørsholm • Denmark Telephone: +45 4516 9200 • [email protected] • www.dhigroup.com • Company Reg. No.: DK36466871

Biological efficacy performance evaluation of Ballast Water Management System Hyde GUARDIAN-US – HG300U in land-based test

Land-based test report

Prepared for DNV GL AS

Represented by Sarah Lasselle DHI Maritime Technology Evaluation Facility

in Hundested, Denmark

Project manager Anne Sofie Kiil

Quality supervisor Torben Madsen

Project number 11822035

Approval date 2019-12-03

Revision Final report / 11822035 / 2019-12-03

Classification Confidential

DHI - BWL-DK / 11822035 / DNV–GL - Hyde Marine / Performance evaluation in land-based test facility / Final report i

CONTENTS

1 Executive summary .................................................................................... 5

2 Introduction ............................................................................................... 15

3 Objective .................................................................................................... 15

4 Independent Laboratory ........................................................................... 16

5 Manufacturer ............................................................................................. 16

6 Testing Organisation ................................................................................ 16

7 Ballast water management system ......................................................... 18 7.1 General system description .................................................................................... 18 7.2 Performance claim and BWMS limitation ............................................................... 18

8 Experimental design ................................................................................. 22 8.1 Trial period, installation and operation of the BWMS.............................................. 22 8.2 BWMS commissioning ............................................................................................ 23 8.3 Biological efficacy verification testing ..................................................................... 23 8.4 Test water and challenge conditions in BE verification testing ............................... 31 8.5 Operation and maintenance testing ........................................................................ 33 8.6 Qualitative operation and maintenance performance indicators ............................ 34

9 Sampling .................................................................................................... 37 9.1 Sample overview ..................................................................................................... 37 9.2 Sampling and analyses of inlet and treated water during ballasting ....................... 38 9.3 Treated discharge water and control discharge water ............................................ 39 9.4 Analyses .................................................................................................................. 40

10 Results from biological efficacy test cycles .......................................... 41 10.1 Amendments and deviations to Test Plan .............................................................. 42 10.2 Inlet and control discharge water ............................................................................ 43 10.3 Treated water .......................................................................................................... 55

11 Quality control and quality assurance .................................................... 65

12 Conclusion ................................................................................................ 68

13 References ................................................................................................ 69

FIGURES Figure 6.1 DHI Maritime Technology Evaluation Facility, Hundested, Denmark. .................... 17

DHI - BWL-DK / 11822035 / DNV–GL - Hyde Marine / Performance evaluation in land-based test facility / Final report ii

Figure 11.1 Graphic illustration of quality control processes in type approval testing of BWMS. ................................................................................................................................ 67

TABLES Table 1.1 Performance claim of Hyde GUARDIAN-US (for HG300U). .................................... 6 Table 1.2 Measurements during the land-based test of parameters with relevance for Hyde

Marine’s performance claim. ..................................................................................... 7 Table 1.3 Test water conditions in fresh, brackish and marine water test cycles. .................. 10 Table 1.4 Average flow rates at inlet and at discharge, UV-T, UV-I and UV dose readings,

and average numbers (three replicates) of live organisms in treated water and control at discharge. Live organisms ≥10 and <50 µm were quantified by microscopy counting after staining with CMFDA/FDA. All counts of pathogenic bacteria (E. coli, enterococci and Vibrio cholerae) in the test cycles were below the ballast water discharge standard. ........................................................................... 12

Table 7.1 UV-T / flow rate relationship. ................................................................................... 19 Table 7.2 Performance claim of Hyde GUARDIAN-US (for HG300U). .................................. 20 Table 7.3 Measurements during the land-based test of parameters with relevance for Hyde

Marine’s performance claim. ................................................................................... 21 Table 8.1 Volumes, flow rates and UV-T UV-I and UV dose at inlet and discharge. ............. 25 Table 8.2 Minimum water quality parameters according to the ETV protocol /5/, and, in

parentheses, the IMO G8 guidelines /2/. ................................................................ 31 Table 8.3 Minimum densities of live organisms in the test water according to the ETV

protocol /5/ and the IMO G8 guidelines /2/. ............................................................ 32 Table 8.4 Details for operation and maintenance (O&M) test cycles. .................................... 34 Table 9.1 Overview of sampling and purpose of samples. ..................................................... 37 Table 9.2 Sampling and analyses of source water from source tank. .................................... 38 Table 9.3 Sampling and analyses of inlet water to BWMS and control retention tank. .......... 38 Table 9.4 Sampling and analyses of treated water during ballasting operation. .................... 39 Table 9.5 Sampling and analysis of treated discharge water and control discharge water. ... 39 Table 10.1 Source, inlet and control discharge waters. Average concentrations (three

replicates) of total suspended solids (TSS), mineral matter (MM), particulate organic carbon (POC), dissolved organic carbon (DOC) and average measurements of UV-transmittance (UV-T). .......................................................... 43

Table 10.2 Inlet and control discharge water. Average measurements of dissolved oxygen, pH, salinity, temperature, turbidity and chlorophyll a. .................................................... 45

Table 10.3 Inlet and control discharge waters. Total sample volumes and average densities of live organisms ≥50 µm. Average of three replicates in inlet water and in control discharge water. ...................................................................................................... 48

Table 10.4 Inlet and control discharge waters. Average densities of live organisms ≥10 µm and <50 µm determined by microscopy counting. Furthermore, the fraction of standard test organisms (STOs) in the inlet (test water) is also provided. ............................ 50

Table 10.5 Densities of live organisms <10 µm. One replicate per event (Inlet to BWMS and Inlet to control) or three replicates (Discharge). ...................................................... 52

Table 10.6 Treated water. Average concentrations (three replicates) of total suspended solids (TSS), mineral matter (MM), particulate organic carbon (POC) and dissolved organic carbon (DOC). ............................................................................................ 56

Table 10.7 Treated water. Average measurements of dissolved oxygen, pH, salinity, temperature, turbidity and chlorophyll a. ................................................................. 57

Table 10.8 Treated water. Total sample volumes and average densities (three replicates) of live organisms ≥50 µm. ........................................................................................... 59

Table 10.9 Treated water. Average densities of live organisms ≥10 µm and <50 µm by microscopy counting (six replicates) ....................................................................... 61

Table 10.10 Treated water. Average bacterial densities (three replicates). ............................... 62

DHI - BWL-DK / 11822035 / DNV–GL - Hyde Marine / Performance evaluation in land-based test facility / Final report iii

APPENDICES

APPENDIX A Detailed data on physical-chemical parameters and biological efficacy analyses

APPENDIX B Test Plan (including QMP version 5.2, QAPP version 5.2), Amendment Nos. 1-3, Deviation Nos.

1-6

APPENDIX C Event log

APPENDIX D Data logging from the land-based test

APPENDIX E Data logging from the operation and maintenance test

APPENDIX F Commissioning verification report

APPENDIX G Quality control and quality assurance

APPENDIX H Summaries of validation studies

DHI - BWL-DK / 11822035 / DNV-GL – Hyde Marine / Performance evaluation in land-based test facility / Final report Page 1 of 181


Biological efficacy performance evaluation of Hyde GUARDIAN-US – HG300U Ballast Water Management System in land-based test

Land-based test report Final Report 11822035 /2019-12-03

Approved by

03-12-2019

XTorben MadsenVice President, BWLSigned by: Torben Madsen

Quality Compliance Statement

This report shall not be reproduced except in full, without written approval of DHI.

The biological efficacy performance evaluation of the ballast water management system (BWMS) was conducted as required in the United States Coast Guard Standards for Living Organisms in Ships’ Ballast Water Discharged in U.S. Waters [33 CFR 151 and 46 CFR 162. Federal Register, Vol. 77, No. 57, March 23, 2012] and the Guidelines for Approval of Ballast Water Management Systems (G8) [IMO G8 guidelines, Resolution MEPC. 279(70), 2016]

The report contains no known errors, omissions or false statements

The main part of the report (pages 1 to 181) was prepared in compliance with the international standard ISO/IEC 17025.

The methods described below were conducted in compliance with ISO/IEC 17025. These methods collectively cover all essential parts of the biological performance evaluation of the BWMS in the land-based test.

DHI SOP Specification of method

30/1738 Sampling – preparation, subsampling and transportation of samples (land-based test)

30/1769 Determination of dissolved organic carbon (DOC) and particulate organic carbon (POC)

30/530 Determination of total suspended solids (TSS)

30/1700 Determination of live organisms ≥50 µm

30/1701 Determination of live organisms ≥10 and <50 µm

30/1704 Most probable number assay with algae

30/1706 Determination of live organisms <10 µm – heterotrophic plate count

30/1708 Determination of live organisms <10 µm – total coliforms, E. coli and enterococci

30/1707 Determination of live organisms <10 µm – Vibrio cholerae


Abbreviations

Abbreviation Description

AVG Average

BE Biological efficacy

BWMS Ballast water management system

CFU Colony-forming units

CMFDA Chloromethylfluorescein diacetate

DANAK Danish Accreditation and Metrology Fund

DHI MTEF DHI Maritime Technology Evaluation Facility

DOC Dissolved organic carbon

DP Differential pressure

ETV US-EPA, Environmental Technology Verification Program

FDA Fluorescein diacetate

FR Field replicate

HMI Human machine interface

IMO International Maritime Organization

LR Laboratory replicate

MEPC Marine Environment Protection Committee

MM Mineral matter

MPN Most probable number

NTU Nephelometric turbidity units

O&M Operation and maintenance

OMSM Operation maintenance and safety manual

PLC Programmable logic controller

POC Particulate organic carbon

PSU Practical salinity units

QA Quality assurance

QAPP Quality Assurance Project Plan

QC Quality control

QMP Quality Management Plan


Abbreviation Description

SOP Standard operating procedure

STD Standard deviation

STO Standard test organisms

T0 Day 0 samples

T1 Day 1 samples

T5 Day 5 samples

TRC Total or treatment rated capacity

TRO Total residual oxidants

TSS Total suspended solids

USCG United States Coast Guard

UV-I Ultraviolet intensity

UV-T Ultraviolet transmittance


1 Executive summary DHI provides independent performance evaluation of ballast water management systems (BWMS) for the approval process. The purpose of the performance evaluation is to assure that BWMS approved by administrations are capable of meeting the ballast water discharge standard in land-based and shipboard tests. The objective of this project was to conduct a biological performance evaluation of the Hyde GUARDIAN-US – HG300U BWMS (hereafter Hyde GUARDIAN-US) manufactured by Hyde Marine Inc. at a land-based facility with the aim to meet the requirements for type approval testing in the United States Coast Guard Standards for Living Organisms in Ships’ Ballast Water Discharged in U.S. Waters [33 CFR 151 and 46 CFR 162. Federal Register, Vol. 77, No. 57, March 23, 2012] and the Guidelines for Approval of Ballast Water Management Systems (G8) [IMO G8 guidelines; Resolution MEPC. 279(70), 2016].

The test waters used for the land-based test cycles in fresh, brackish and marine waters were prepared in accordance with the Generic Protocol for the Verification of Ballast Water Treatment Technology [ETV protocol; U.S. Environmental Protection Agency, Environmental Technology Verification Program, September 2010] and the IMO G8 guidelines and complied with the requirements excepted from situations described in deviations to the Test Plan. During the performance evaluation of the Hyde GUARDIAN-US, the testing procedures and the applied quality assurance (QA) and quality control (QC) were consistent with the U.S. Coast Guard requirements, the ETV protocol and the IMO G8 guidelines. Furthermore, DHI’s testing procedures, and particularly the preparation of test waters, were supported by internal validation studies [DHI. Summaries of validation studies of conditions and procedures applied in type approval tests of ballast water management systems; included in Appendix H].

From 10 July to 25 October 2018, DHI conducted a land-based test of the Hyde GUARDIAN-US with DNV GL as the Independent Laboratory. The Hyde GUARDIAN-US was equipped with a 20 µm Filtrex ACB 945 during this land-based evaluation.

The basic treatment principles of the Hyde GUARDIAN-US were mechanical filtration and treatment by ultraviolet (UV) radiation.

The BWMS applied a Filtrex 20 µm filter to remove organisms and suspended matter. The organisms and suspended matter collected by the filtration unit are returned to the ambient water by an auto back-flushing function. The filtration unit is bypassed during de-ballasting.

Before initiation of the land-based test, Hyde Marine described a technology performance claim including limitations for the treatment performance of the Hyde GUARDIAN-US, which was included in Section 4.1 of the Test Plan (Appendix B). The full performance claims are provided in section 7.2 and a summary of the performance claims are reproduced below in italics:

1. The Hyde GUARDIAN-US meets the treatment and operation standards (with reference to the information required in the ETV protocol, Section 3.2) as:

a. BWMS designed to accomplish the ballast water discharge standard in IMO G8 (Revised) and U.S. Coast Guard Standards, §151.2030

b. A BWMS meeting the design and construction standards of DNV GL as a Type Approved BWMS as well as the requirements of 46 CFR for installation onboard US flagged vessels.

2. The biological treatment efficacy stated above can be achieved by the following quantitative measures of operational performance:

a. Maximum Treatment Rated Capacity (TRC) for the supplied BWMS unit: 300 m3/h


b. A calculated ultraviolet dose (UV dose) of minimum 170 mJ/cm2 based upon calculations of flow and the measured ultraviolet intensity (UV-I) in the chamber up to a full flow of the TRC

3. The maritime environmental conditions where the BWMS can be expected to achieve the ballast water discharge standard:

a. Salinity range: the BWMS works in any salinity b. Temperature: the BWMS works with water temperatures from 0 to 40 °C c. Ultraviolet transmittance (UV-T): the BWMS works with full flow rate from UV-T

(unfiltered) (measured in percentage) of 100 down to approximately 77. Flow regulating valve V4 automatically reduces flow when calculated UV dose drops below 170 mJ/cm2 down to an observed minimum flow corresponding to 15% minimum opening of V4. If a UV dose of minimum 170 mJ/cm2 cannot be achieved a treatment alarm and low UV intensity alarm is activated. It is expected that unfiltered UV-T values for water at the extreme low end of system performance is 48%. See Table 7.1 for estimated UV-T/flow relationship

4. Concentration of disinfection residuals, by-products and toxicity for relevant systems:

a. No disinfection residuals, by-products or toxicity are expected in the discharge water

5. The required operational and maintenance conditions (operator time, power requirements, chemical consumption requirements, reliability, etc.)

a. Power range: system has two power settings: 100% power (Level 2) and 60% power (Level 1). Level 1 power is only when UV-T of the water is excess of 93 UV-T (filtered)

b. Hold time: no hold time is required for treatment c. Operator Time: no operator time is required; the system is fully automatic and will

function without user interface once system is started. No manual valve actuation is necessary

d. Maintenance requirements: see OMSM for maintenance details

Table 1.1 Performance claim of Hyde GUARDIAN-US (for HG300U).

Parameter Units USCG (HG300U-SC)

Maximum flow rate (TRC) m3/h 300

Minimum flow rate (for lamp cooling)

m3/h 48

Minimum flow rate (based on low UV-T of water)

m3/h 68

Power consumption (total system max /nominal power

kWh/m3 0.192 / 0.112

Minimum UV dose Set Point mJ/cm2 170

Low flow UV-T-limit (unfiltered)

% cm (at 254 nm) 49

Full flow UV- T -limit (unfiltered)

% cm (at 254 nm) 77

Low power level set point % power 60% (3.6 kW/lamp)

High power level set point % power 100% (6.0 kW/lamp)


Holding time h Not applicable

Maximum Allowable Working Pressure

bar 10

Expected backwash volume m3/h 15 (during backflush)

Temperature °C 0 – 40

Salinity PSU 0 – 36+

During the land-based test, the aim was to test the Hyde GUARDIAN-US equipped with the 20 µm Filtrex ACB 945 filter according to Hyde Marine’s performance claim. Table 1.2 summarises the results of the measurements obtained during the land-based test that are related to the performance claim.

Table 1.2 Measurements during the land-based test of parameters with relevance for Hyde Marine’s performance claim.

Test cycle

Salinity (PSU)

UV-T (%)1)

UV dose readings (mJ/cm2)2)

Ballast

UV-dose readings (mJ/cm2)3)

De-ballast

Flow rate4) (m3/h)4)

Holding time (Days)

Power consumption (kWh/100 m3)5)

F-1 0.36 48 (72) 163 - 202 182 ± 13

176 - 182 179 ± 2

93 1 63

F-2 0.33 48 (70) 136 - 264 179 ± 21

174 - 186 179 ± 3

90 1 68

F-3 0.37 59 (77) 190 - 280 222 ± 21

176 - 183 179 ± 2

118 5 50

F-4 0.36 55 (75) 173 - 273 213 ± 31

177 - 202 180 ± 5

108 1 54

F-5 0.36 56 (74) 174 - 222 187 ± 12

176 - 183 178 ± 2

111 5 53

B-1 17 68 (88) 186 - 219 197 ± 9

177 - 180 178 ± 1

199 1 30

B-2 17 65 (86) 185 - 200 191 ± 3

172 - 190 178 ± 5

187 1 31

B-3 20 52 (71) 156 - 190 178 ± 7

174 - 184 179 ± 2

99 1 59

B-4 22 61 (82) 205 - 237 215 ± 9

177 - 186 179 ± 2

125 5 48

B-5 22 61 (82) 217 - 250 229 ± 9

131 - 180 175 ± 13

116 5 51

B-6 20 51 (68) 128 - 564 227 ± 105

127 - 295 185 ± 35

70 1 84


Test cycle

Salinity (PSU)

UV-T (%)1)

UV dose readings (mJ/cm2)2)

Ballast

UV-dose readings (mJ/cm2)3)

De-ballast

Flow rate4) (m3/h)4)

Holding time (Days)

Power consumption (kWh/100 m3)5)

B-7 19 50 (65) 166 - 304 209 ± 35

174 - 184 179 ± 2

52 1 115

M-1 29 50 (69) 167 - 192 180 ± 6

172 - 292 183 ± 23

92 1 64

M-2 29 73 (88) 173 - 181 178 ± 3

175 - 191 180 ± 4

223 1 27

M-3 29 73 (88) 174 - 187 181 ± 5

177 - 193 180 ± 5

235 1 25

M-4 30 61 (82) 165 - 227 187 ± 16

172 - 180 178 ± 2

140 5 42

M-5 30 61 (82) 177 - 227 190 ± 15

176 - 184 179 ± 2

137 5 42

1) UV-T measured during ballast operation; numbers in parentheses indicate value obtained after the sample was filtered through a 0.45-µm syringe filter upon receipt at the DHI laboratory

2) UV dose range measured at stable operating conditions (minimum and maximum) and average value ± standard deviation logged during ballast operation; the data were extracted from the BWMS log

3) UV dose range (minimum and maximum) measured at stable operating conditions and average value ± standard deviation logged during de-ballast operation; the data were extracted from the BWMS log

4) Calculated flow rate (as processed volume by operation time) measured after filtration during ballast operation 5) Power consumption measured during ballast operation for the total system

The land-based test was performed in accordance to DHI’s Quality Assurance Project Plan (QAPP version 5.2, included in the Test Plan, Appendix B). A total of 17 biological efficacy (BE) test cycles (5 marine water, 5 fresh water and 7 brackish water) were conducted, and 5 operation and maintenance (O&M) test cycles with a total of 53 operation hours were performed. The following pairs of test cycles were run consecutively by use of the same test water and the same control: BE test cycles B-4 and B-5; BE test cycles M-2 and M-3 and BE test cycles M-4 and M-5.

The Hyde GUARDIAN-US was operated independently by DHI staff during all BE test cycles and O&M test cycles. Staff from Hyde Marine were present at the test facility during several of the BE test cycles but did not participate in the test.

Table 1.3 summarises the test water conditions applied for the three water qualities, i.e., fresh, brackish and marine waters, and includes information on salinity, temperature, specific information on physical-chemical augmentation (dissolved organic carbon (DOC), particulate organic carbon (POC) and total suspended solid (TSS)) and live organisms (total count and standard test organisms (STOs) in percent of total count).

Table 1.4 summarises the flow rates, UV-T, UV-I and UV dose during the inlet (ballast operations) and flow rates, UV-I and UV dose during the discharge (de-ballast operations) in the individual BE test cycles. Furthermore, the numbers of live organisms at discharge in water treated by the Hyde GUARDIAN-US and in the control are shown for the BE test cycles. The results on the ≥10 and <50 µm size class included evaluations of the samples by microscopy counting after staining with chloromethylfluorescein diacetate (CMFDA) and fluorescein diacetate (FDA).


During the testing period three amendments and six deviations to the Test Plan were prepared. The full amendments and deviations can be found in Appendix B:

• Amendment No. 1 describes a revised sampling methodology for test cycles with flow rates lower than 100 m3/h. Thus, sequential sampling of live organisms ≥50 μm, live organisms ≥10 and <50 μm and live organisms <10 μm was performed in eight BE test cycles

• Amendment No. 2 describes the use of Staurastrum sp. as a freshwater STO in the ≥10 and <50 µm size class when needed to fulfil the density requirements of organisms ≥10 and <50 µm in the inlet water (≥1,000 organisms/mL). This STO was used in two BE test cycles

• Amendment No. 3 describes (1) renaming of the BWMS model (the model name HG3A was used in the original Test Plan; the new name HG300U is used in this report) and (2) revision of the performance claim. Temperature high limit values were corrected to 0-40°C and Low flow UV-T limit was corrected to 48% throughout the performance claim. Furthermore, corrections were made to the text.

• Deviation Nos. 1 and 2 describe two BE test cycles that were run with treated discharge water volumes of 194 m3 and 198 m3, i.e., up to 3% lower than the required 200 m3

• Deviation No. 3 describes four BE test cycles, where online monitoring data were lost during part of the tests

• Deviation No. 4 describes an operation and maintenance test cycle which was performed after the last BE test cycle to reach >50 hours accumulated run time.

• Deviation No. 5 describes erratic chlorophyll a measurements in six BE test cycles for the water sampled on the inlet side of the BWMS during ballast and de-ballast operations (inlet and before second treatment respectively)

• Deviation No. 6 describes two brackish water BE test cycles performed on the same day, where the salinity of the test water was 22 PSU and, thus, exceeded the requirement of 10- 20 PSU with 10%

The brackish water test cycles B-3 and B-6 were considered invalid by DNV GL with the following arguments:

“The reasons for B-3 failure was that the V4 regulation was preventing the system from stabilizing correctly after backwashing and reaching its claimed dose.” /email from DNV-GL, dated 05.03.2018/

“For B-6 this was the test in which the V4 failed to regulate flow due to software bug leading to an internal self-correct control loop. The UV-dose was thus not stably achieved, and Hyde had to update the software.” /email from DNV-GL, dated 05.03.2018/

These events were followed by a software update to the outlet flow restricting valve V4 (after BE test cycle B-3) and a parameter update on valve V4 (after BE test cycle B-6).

The composition of the test waters was considered compliant with the requirements in all 17 BE test cycles with fresh, brackish and marine water (Table 1.3 and Table 1.4). The Hyde GUARDIAN-US equipped with the filter Filtrex ACB 945 20 µm complied with the ballast water discharge standard in the 15 valid BE test cycles, five in each of the salinities fresh, brackish and marine water, when the treatment efficacy towards organisms ≥10 and <50 µm was evaluated by use of the results obtained by microscopy counting of CMFDA/FDA stained organisms.


Table 1.3 Test water conditions in fresh, brackish and marine water test cycles.

Test cycle

Temp. (˚C)

Salinity (PSU)

UV-T (%)1)

DOC (mg/L)

POC (mg/L)

TSS (mg/L)

Organisms ≥50 µm (organisms/m3)

Organisms ≥10 and <50 µm (organisms/mL)

Test water

Test water

Source water

Added DOC2)

Test water

Source water

Test water3)

Source water

Test water4)

Inlet to BWMS

% STOs Inlet to BWMS

% STOs5)

F-1 23 0.36 48 (72) 7.86) - 7.8 1.36) 7.3 35 66 1,185,134 0 2,108 0.40

F-2 22 0.33 48 (70) 8.76) - 8.5 0.876) 7.2 -7) 65 1,794,978 0 1,801 0

F-3 16 0.37 59 (77) 6.16) SC 6.5 0.546) 7.6 15 56 488,056 0 1,132 0

F-4 16 0.36 55 (75) 6.76) - 6.6 0.756) 6.8 16 62 361,056 0 1,492 0

F-5 17 0.36 56 (74) 7.16) - 7.1 0.626) 7.8 14 52 697,556 0 1,230 12

B-1 20 17 68 (88) 3.3 SC 7.1 0.8 7.5 13 72 544,609 0 1,102 42

B-2 22 17 65 (86) 3.8 SC 7.8 1.0 8.3 7.4 65 207,161 0 2,347 3

B-3 18 20 52 (71) 3.4 SC + LS 12 0.91 8.2 6.7 64 334,561 0 2,433 81

B-4 14 22 61 (82) 2.9 SC + LS 8.8 1.0 7.8 13 68 165,828 0 1,073 43

B-5 14 22 61 (82) 2.9 SC + LS 8.8 1.0 7.8 13 68 184,494 0 1,045 43


Test cycle

Temp. (˚C)

Salinity (PSU)

UV-T (%)1)

DOC (mg/L)

POC (mg/L)

TSS (mg/L)

Organisms ≥50 µm (organisms/m3)


Test water

Test water

Source water

Added DOC2)

Test water

Source water

Test water3)

Source water

Test water4)

Inlet to BWMS

% STOs Inlet to BWMS

% STOs5)

B-6 13 20 51 (68) 4.2 SC + LS 14 0.86 8.3 15 68 172,408 0 1,515 38

B-7 12 19 50 (65) 2.9 SC + LS 15 0.36 6.9 4.3 58 115,789 0 1,378 13

M-1 24 298) 50 (69) 4.0 SC + LS 14 1.6 7.5 12 81 302,878 0 3,590 0

M-2 18 298) 73 (88) 3.3 SC 7.2 0.53 5.8 6.3 44 252,883 0 1,310 66

M-3 17 298) 73 (88) 3.3 SC 7.2 0.53 5.8 6.3 44 271,806 0 1,360 66

M-4 13 308) 61 (82) 3.2 SC + LS 8.3 0.63 6.2 6.2 64 224,961 0 1,497 32

M-5 14 308) 61 (82) 3.2 SC + LS 8.3 0.63 6.2 6.2 64 211,472 0 1,487 32

1) UV-T measured during ballast operation; numbers in parentheses indicate value obtained after the sample was filtered through a 0;45-µm syringe filter upon receipt at the DHI laboratory 2) Minimum water quality characteristics were achieved by addition of sodium citrate (SC). Additional lignin sulphonate (LS) was added to adjust the UV-T when specific UV-T targets were

requested 3) Minimum water quality requirements were achieved by addition of corn starch 4) Minimum water quality requirements were achieved by addition of kaolin clay 5) The standard test organisms (STOs) percentage was calculated based on the number of STOs and total organisms count in the full test water volume contained in the source tank 6) DOC and POC determined for the source fresh water is based on individual measurements in tap water and Lake Arresø water 7) Analytical error. Sample disregarded 8) Required salinity was achieved by addition of brine


Table 1.4 Average flow rates at inlet and at discharge, UV-T, UV-I and UV dose readings, and average numbers (three replicates) of live organisms in treated water and control at discharge. Live organisms ≥10 and <50 µm were quantified by microscopy counting after staining with CMFDA/FDA. All counts of pathogenic bacteria (E. coli, enterococci and Vibrio cholerae) in the test cycles were below the ballast water discharge standard.

Test cycle

Inlet water Discharge water Organisms ≥50 µm (organisms/m3)


Average flow rate (m3/h)1)

UV-T (%)

UV-I (W/m2)

UV dose readings (mJ/cm2)

Average flow rate (m3/h)

UV-T (%)

UV-I (W/m2)


Treated Control Treated Control

Treated Control Test water

Applied during ballast2)

Treated Control Treated Applied during de-ballast2)

F-1 108 200 48 (72) 209 - 220 213 ± 3

163 - 202 182 ± 13

103 200 51 (72) 221 - 234 226 ± 3

176 - 182 179 ± 2

0 528,022 7.6 2,388

F-2 101 199 48 (70) 205 - 217 209 ± 3

136 - 264 179 ± 21

97 201 49 (71) 209 - 218 212 ± 2

174 - 186 179 ± 3

1.5 712,876 7.8 1,490

F-3 128 200 59 (77) 191 - 322 311 ± 27

190 - 280 222 ± 21

171 198 71 (78) 367 - 391 382 ± 7

176 - 183 179 ± 2

0 352,607 0 629

F-4 120 200 55 (75) 277 - 290 282 ± 4

173 - 273 213 ± 31

132 205 57 (76) 294 - 317 297 ± 5

177 - 202 180 ± 5

0 226,483 8.2 1,235

F-5 122 199 56 (74) 267 - 282 272 ± 4

174 - 222 187 ± 12

152 200 67 (75) 268 - 282 274 ± 3

176 - 183 178 ± 2

2.7 360,426 1.0 664

B-1 254 201 68 (88) 454 - 497 486 ± 10

186 - 219 197 ± 9

234 233 75 (89) 518 - 533 525 ± 4

177 - 180 178 ± 1.0

0.33 150,918 0.17 749

B-2 247 249 65 (86) 452 - 473 457 ± 5

185 - 200 191 ± 3

223 224 71 (88) 444 - 504 490 ± 22

172 - 190 178 ± 5

0 98,235 0.67 1,321

B-33) 103 199 52 (71) 218 - 230 226 ± 3

156 - 190 178 ± 7

100 201 52 (69) 222 - 227 224 ± 2

174 - 184 179 ± 2

0.33 102,300 17.7 (0.56) 4)

1,163


Test cycle

Inlet water Discharge water Organisms ≥50 µm

(organisms/m3)



UV-T (%)

UV-I (W/m2)



UV-T (%)

UV-I (W/m2)






B-45) 138 300 61 (82) 338 - 347 343 ± 2

205 - 237 215 ± 9

170 189 71 (81) 343 - 392 381 ± 13

177 - 186 179 ± 2

0.33 92,844 0.33 515

B-5 132 300 61 (82) 334 - 346 341 ± 3

217 - 250 229 ± 9

172 189 72 (81) 292 - 393 381 ± 26

131 - 180 175 ± 13

1.0 92,844 1.7 515

B-66) 78 299 51 (68) 154 - 194 187 ± 7

128 - 564 227 ± 105

85 297 53 (65) 184 - 194 190 ± 3

127 - 295 185 ± 35

2.7 127,679 10.1 (0.44)4)

1,159

B-7 56 301 50 (65) 124 - 142 133 ± 4

166 - 304 209 ± 35

53 284 49 (60) 116 - 124 119 ± 2

174 - 184 179 ± 2

3.3 54,634 5.1 1,382

M-1 106 201 50 (69) 204 - 224 210 ± 5

167 - 192 180 ± 6

98 199 52 (67) 188 - 219 217 ± 6

172 - 292 183 ± 23

0 83,615 1.2 354

M-25) 232 234 73 (88) 333 - 528 508 ± 55

173 - 181 178 ± 3

246 244 77 (89) 501 - 556 546 ± 16

175 - 191 180 ± 4

1.0 165,626 2.7 784

M-3 240 234 73 (88) 523 - 529 526 ± 2

174 - 187 181 ± 5

243 244 77 (89) 499 - 551 542 ± 16

177 - 193 180 ± 5

0 165,626 1.8 784

M-45) 151 202 61 (82) 332 - 350 340 ± 5

165 - 227 187 ± 16

170 194 72 (82) 355 - 390 384 ± 10

172 - 180 178 ± 2

0.67 72,283 0 917


Test cycle

Inlet water Discharge water Organisms ≥50 µm (organisms/m3)



UV-T (%)

UV-I (W/m2)



UV-T (%)

UV-I (W/m2)






M-5 144 202 61 (82) 325 - 334 330 ± 3

177 - 227 190 ± 15

154 194 70 (82) 330 - 351 347 ± 6

176 - 184 179 ± 2

0 72,283 0.17 917

1) Average flow rate was measured before filtration 2) UV-I and UV dose range measured at stable operating conditions (minimum and maximum) and average value ± standard deviation logged during ballast operation; the data were extracted

from the BWMS log 3) BE test cycle B-3 was considered invalid by DNV GL 4) Average number of organisms ≥10 and <50 μm after deduction of false positives. The average numbers of false positive organisms ≥10 and <50 μm in treated discharge, which were deducted

from the stated value, are provided in parentheses 5) This test cycle and the following test cycle were run consecutively by use of the same test water and the same control 6) BE test cycle B-6 was considered invalid by DNV-GL/

***

DNV GL AS Revised final Report

December 2019

Biological efficacy performance evaluation of Hyde GUARDIAN-US HG750U Ballast Water Management System in shipboard test

Hyde Marine Inc.

© Shutterstock / Alex Kolokythas Photography


DHI - BWL-DK / 11822035 / DNV GL - Hyde / Performance evaluation in shipboard testing / Report

This report has been prepared under the DHI Business Management System certified by Bureau Veritas to comply with ISO 9001 (Quality Management)

Approved by

18-12-2019

XApproved by


DHI A/S • Agern Allé 5 • DK-2970 Hørsholm • Denmark Telephone: +45 4516 9200 • [email protected] • www.dhigroup.com • Company Reg. No. DK36466871

Biological efficacy performance evaluation of Hyde GUARDIAN-US HG750U Ballast Water Management System in shipboard test

Hyde Marine Inc.

Prepared for DNV GL AS Represented by Ms Sarah Lasselle, Engineer

© Shutterstock / Alex Kolokythas Photography

Project manager Aron Lank Jensen

Quality supervisor Torben Madsen

Project number 11822035

Approval date 2019-12-18

Revision Revised final report 11822035 / 2019-12-18 (1st version issued 2019-12-12)

Classification Confidential

DHI - BWL-DK / 11822035 / DNV GL - Hyde / Performance evaluation in shipboard testing / Report Page i

CONTENTS

1 Executive summary................................................................................... 3

2 Introduction ............................................................................................... 9

3 Objective .................................................................................................... 9

4 Independent Laboratory ........................................................................... 9

5 Manufacturer ............................................................................................. 9

6 Testing Organisation .............................................................................. 10

7 Description of the ballast water management system ......................... 10 7.1 General system description .................................................................................... 10 7.2 Performance claim and BWMS limitation ............................................................... 10 7.3 Technology and process description ...................................................................... 12

8 Experimental design, trial period and locations ................................... 12

9 Sampling .................................................................................................. 15 9.1 Sample overview ..................................................................................................... 15 9.2 Isokinetic sampling .................................................................................................. 16 9.3 Samples for DOC, POC, UV-T and TSS analyses ................................................. 16 9.4 Samples for enumeration of organisms ≥50 µm ..................................................... 16 9.5 Samples for enumeration of organisms ≥10 and <50 µm and organisms <10 µm . 16

10 Analyses and data collection ................................................................. 17 10.1 Analysis overview.................................................................................................... 17 10.2 Physical-chemical analyses .................................................................................... 18 10.3 Organism size class ≥50 µm ................................................................................... 18 10.4 Organism size class ≥10 µm and <50 µm .............................................................. 19 10.5 Organism size class <10 µm ................................................................................... 19 10.6 Data collection during testing period ....................................................................... 20

11 Results on inlet and discharge waters .................................................. 20 11.1 Physical-chemical properties .................................................................................. 20 11.2 Organism size class ≥50 µm ................................................................................... 23 11.3 Organism size class ≥10 and <50 µm ..................................................................... 24 11.4 Organism size class <10 µm (bacteria) .................................................................. 26

12 Amendments and deviations to Test Plan ............................................. 27

13 Quality control and quality assurance ................................................... 28

14 Conclusion .............................................................................................. 31

15 References ............................................................................................... 31

DHI - BWL-DK / 11822035 / DNV GL - Hyde / Performance evaluation in shipboard testing / Report Page ii

FIGURES Figure 13.1 Graphic illustration of quality control processes in type approval testing of BWMS.

................................................................................................................................ 30

TABLES Table 1.1 Locations of the ballast and deballast operations, dates and holding time for the

shipboard test cycles with the Hyde GUARDIAN-US BWMS. .................................. 5 Table 1.2 Inlet water conditions during ballast operations. ....................................................... 6 Table 1.3 Average flow rates and UV-T and UV-I during ballast and deballast operations and

average numbers of live organisms in treated discharge water. .............................. 8 Table 8.1 Details for inlet and discharge operation for the shipboard test cycles. Detailed data

logging information for each test cycle is available in Appendix C. ........................ 13 Table 9.1 Overview and purpose of samples.......................................................................... 15 Table 10.1 Analyses of inlet water and discharge water. ......................................................... 17 Table 11.1 Inlet and discharge waters. Average concentrations (three replicates) of total

suspended solids (TSS), mineral matter (MM), particulate organic carbon (POC), dissolved organic carbon (DOC) and average measurements of UV-transmittance (UV-T). .................................................................................................................... 21

Table 11.2 Inlet and discharge waters. Average measurements of oxygen saturation, pH, salinity, temperature and turbidity. .......................................................................... 22

Table 11.3 Inlet and discharge waters. Total sample volumes and average densities of live organisms ≥50 µm. Average of three replicates in inlet water and in discharge water, except for test cycles Nos. 9 and 10 (See Deviation to Test Plan No. 1, Appendix B). Specific data and individual sample volumes are provided in Appendix A, Section A.2.1 ...................................................................................... 23

Table 11.4 Inlet and discharge waters. Average densities of live organisms ≥10 µm and <50 µm in the inlet water and the treated discharge water determined by microscopy counting ................................................................................................................... 25

Table 11.5 Inlet and discharge waters. Average densities of live organisms <10 µm in the inlet water (three subsamples from one replicate) and the discharge water (three subsamples from one replicate). Specific data including storage temperatures during transportation are provided in Appendix A, Section A.4. ............................. 26

APPENDICES

APPENDIX A Detailed data on physical-chemical parameters and biological analyses

APPENDIX B Test Plan (including QMP version 5.2, QAPP version 5.2), Amendment Nos. 1-3, Deviation No. 1, current QMP version 6.1 and QAPP version 5.3

APPENDIX C Data logging

APPENDIX D Quality control and quality assurance

APPENDIX E Hyde Marine shipboard testing documentation

APPENDIX F Hyde Marine contamination report

DHI - BWL-DK / 11822035 / DNV GL - Hyde / Performance evaluation in shipboard testing / Report Page 1 of 64

Biological efficacy performance evaluation of Hyde GUARDIAN-US HG750U Ballast Water Management System in shipboard test Shipboard test report Revised final report 11822035 / 2019-12-18

Approved by 18-12-2019

XTorben Madsen

Vice President, BWL


Quality compliance statement This report shall not be reproduced except in full, without written approval of DHI A/S (hereinafter referred to as “DHI”).

• The shipboard performance evaluation of the ballast water management system(BWMS) was conducted as required in the United States Coast Guard Standards forLiving Organisms in Ships’ Ballast Water Discharged in U.S. Waters [33 CFR 151and 46 CFR 162. Federal Register, Vol. 77, No. 57, March 23, 2012] and the BallastWater Management Convention and the BWMS Code [Ballast Water ManagementConvention and BWMS Code; Resolution MEPC.300(72), April 13, 2018]

• The report contains no known errors, omissions or false statements

The main part of the report (pages 1 to 64) was prepared in compliance with the international standard ISO/IEC 17025.

The revised final report was issued as response to request from DNV GL for minor revision of the description of the performance claim:

• Statement for holding time changed to “Not applicable” (instead of “No claim)• Correct minimum UV-T of 48% (instead of 49%)• Correct power consumption of the UV lamps at 60% power of 4.6 kW/lamp (instead

of 5.7 kW/lamp)

The methods described below were conducted in compliance with ISO/IEC 17025. These methods collectively cover all essential parts of the biological performance evaluation of the BWMS in the shipboard test.

DHI SOP Specification of method

30/1773 Shipboard test - Operation, sampling, data-log

30/1769 Determination of dissolved organic carbon (DOC) and particulate organic carbon (POC)

30/530 Determination of total suspended solids (TSS)

30/1700 Determination of live organisms ≥50 µm

30/1701 Determination of live organisms ≥10 and <50 µm

30/1708 Determination of live organisms <10 µm – total coliforms, E. coli and enterococci

30/1707 Determination of live organisms <10 µm – Vibrio cholerae


Abbreviations Abbreviation Description

AVG Average

BE Biological efficacy

BWMS Ballast water management system

CFU Colony-forming units

CMFDA Chloromethylfluorescein diacetate

DANAK The Danish Accreditation Fund

DOC Dissolved organic carbon

ETV US-EPA, Environmental Technology Verification Program

FDA Fluorescein diacetate

FNU Formazin Nephelometric Unit

FR Field replicate

IMO International Maritime Organization

LR Laboratory replicate

MEPC Marine Environment Protection Committee

MM Mineral matter

MPN Most probable number

NA Not Available

OMSM Operation maintenance and safety manual

POC Particulate organic carbon

PSU Practical salinity units

QA Quality assurance

QAPP Quality Assurance Project Plan

QC Quality control

QMP Quality management plan

SOP Standard operating procedure

STD Standard deviation

TRC Total or treatment rated capacity

TSS Total suspended solids

USCG United States Coast Guard

UV-I Ultraviolet intensity

UV-T Ultraviolet transmittance


1 Executive summary DHI provides independent performance evaluation of ballast water management systems (BWMS) for the approval process. The purpose of the performance evaluation is to assure that BWMS approved by administrations are capable of meeting the ballast water discharge standard in land-based and shipboard tests.

The objective of this project was to conduct a biological performance evaluation in a shipboard test of the Hyde GUARDIAN-US HG750U BWMS manufactured by Hyde Marine Inc. with the aim to meet the requirements for type approval testing in the United States Coast Guard (USCG) Standards for Living Organisms in Ships’ Ballast Water Discharged in U.S. Waters [33 CFR 151 and 46 CFR 162. Federal Register, Vol. 77, No. 57, March 23, 2012] hereafter referred as U.S. Coast Guard standards and the BWMS Code [Code for Approval of Ballast Water Management Systems (BWMS Code); Resolution MEPC.300(72), April 13, 2018]. The system evaluated in the present report will hereafter be described as the Hyde GUARDIAN-US BWMS. During the performance evaluation of the Hyde GUARDIAN-US BWMS, the applied quality assurance (QA) and quality control (QC) procedures were consistent with the U.S. Coast Guard requirements, and the sampling and analysis were conducted in accordance with the principles described in the Generic Protocol for the Verification of Ballast Water Treatment Technology (ETV protocol) [U.S. Environmental Protection Agency, Environmental Technology Verification Program, September, 2010].

From 5 November 2018 to 2 October 2019, DHI conducted a shipboard test of the Hyde GUARDIAN-US BWMS on board the Ro-Ro Cargo Ship, Bore Sea (IMO 9443554), with DNV GL as the Independent Laboratory.

The basic treatment principles of the Hyde GUARDIAN-US BWMS were mechanical filtration and treatment by ultraviolet (UV) radiation. During this shipboard evaluation, the Hyde GUARDIAN-US BWMS was equipped with a 20 µm Filtrex filter (ACB-985-300, NiAlBz construction, dP setting: 0.3 bar) to remove organisms and suspended matter. The organisms and suspended matter collected by the filtration unit are returned to the ambient water by an auto back-washing function. The UV unit (Calgon Carbon UV20E Reactor) was equipped with 18 medium pressure UV lamps, totalling 139 kW. The BWMS used power regulation and flow regulation to maintain a constant UV dose.

Mechanical filtration and disinfection with UV radiation were applied during ballast operations, whereas only UV radiation was applied during deballast operations.

Before initiation of the shipboard test, Hyde Marine described a technology performance claim including limitations for the treatment performance of the Hyde GUARDIAN-US BWMS, which was included in Section 4.1 of the Test Plan (Appendix B). The performance in the Test Plan is reproduced below:

Performance claim and BWMS limitation

Hyde Marine states that the BWMS meets the following treatment and operation standards (with reference to the information required in the ETV protocol /4/, Section 3.2):

• The BWMS is designed to accomplish the ballast water discharge standard in BWMS Code /1/ and U.S. Coast Guard Standards /3/, §151.2030, which should be supported by quantitative measures of biological treatment efficacy expressed as a concentration upon discharge of the specified organism size classes

The biological treatment efficacy stated above can be achieved by the following quantitative measures of operational performance:


Performance Claim of Hyde Guardian-US: • Model Number: HG750U• Treatment Rated Capacity: 750 m3/h• Minimum Dose Set Point: 170 mJ/cm2 (calculated MS2RED)• Minimum Flow (approximate, based on low UV-T of water): 80 m3/h• Minimum Flow (approximate, for lamp cooling): 70 m3/hr• UV-T set point: None• Minimum expected UV-T (unfiltered) minimum flow, max power: 48%• Minimum expected UV-T (unfiltered) for max flow at max power: 77%• Maximum allowed UV-T: 100%• Power Level Set Point: 60% power (4.6 kW/lamp x 18 lamps) 100% power (7.7

kW/lamp x 18 lamps)• Power Level Switching Point: GUARDIAN Power Level Switching Diagram in

Functional Description• Power Consumption (Total System, Max / Nominal Power): 148 / 112 kW• Expected Backwash Volume: 80 m3/h• Salinity range: No limitation• Temperature range: 0 – 40 ºC• Holding time: Not applicable• UV-transmission: The BWTS works with full flow rate from unfiltered UV-T

(measured in percentage) of 100 down to approximately 77. Flow valve V4 willautomatically reduce flow when calculated UV dose drops below 170 mJ/cm2 downto a minimum flow of approximately 80 m3/h (15% minimum opening of V4). If a UVdose of minimum 170 mJ/cm2 cannot be achieved a treatment alarm and low UVintensity alarm will be activated. It is expected that unfiltered UVT values for water atthe extreme low end of system performance is 48%

List of Major Components: • UV Chamber: Calgon Carbon UV20E Reactor, 316L Stainless Steel, DN300

Flanges, with internal surface preparation, pickling and passivation• Lamp Assembly: Hyde Marine Part G300780• Filter: Filtrex ACB-985-300, 20-μm mesh, NiAlBz construction• Power Panel: Calgon Carbon power cabinet, G104494, 440 V, 60 Hz• Control Panel: G103232 Panel with Siemens Simatic S700 HMI, Siemens 1215 PLC• Valves: Ghibson valves with pneumatic positions (supplied by Ghibson) and Burkert

positioner for modulating V4 valve• Flow Meter: Endress + Hauser PROMAG W magnetic flow meter, Serial

M7015E160000• UV Intensity Sensor: Hyde Marine Part G350792 Duty, G350793 Reference• Pressure Transmitter: Hyde Marine Part G400709• Software version: Bore Sea: Rev 4F.02.01.00

Reliability of Components: • Reactor: 5,000 hours maintenance-free operation• Lamps: 5,000 hours maintenance-free operation• Filter cartridge: 5,000 hours or 5 years maintenance-free operation

Anticipated maintenance at test site: • Quarterly recalibration of UV Duty Sensor

Anticipated Recalibration: • UV Intensity Sensor recalibration once per three months or more often if observed

values are lower than expected• Pressure Transmitter and Flow Meter as per vessel calibration requirements


A total of 11 shipboard biology efficacy (BE) test cycles were conducted on board the vessel Bore Sea. The Hyde GUARDIAN-US BWMS was operated by the vessel crew during all shipboard test cycles. DHI collected information relevant for the shipboard test cycles and recorded operational observations when DHI staff was present during BE testing. DHI staff members were only present on the vessel during the shipboard test cycles and did not witness scheduled and unscheduled system maintenance performed on the Hyde GUARDIAN-US BWMS during the shipboard testing period. Representatives from the Independent Laboratory were present during test cycle No. 1. Representatives from Hyde Marine were present during all test cycles.

The shipboard test cycles with the Hyde GUARDIAN-US BWMS were conducted in ports in Lübeck (DE), Gdynia (PL), Hanko (FI), St. Petersburg (RU) and en route to locations in the Baltic Sea and the Gulf of Finland. The holding time varied from approx. 14 to 37 hours for treated water. The Hyde GUARDIAN-US BWMS was tested at salinities ranging from 0.18 to 17 PSU with water temperatures ranging from 1.5 to 22°C.

Table 1.1 summarizes the locations of the ballast operations, dates and holding time of the shipboard test cycles.

Table 1.1 Locations of the ballast and deballast operations, dates and holding time for the shipboard test cycles with the Hyde GUARDIAN-US BWMS.

Test cycle

Ballast location 1) Deballast location 1) Inlet (UTC) Date and time

Discharge (UTC) Date and time

Holding time 1)

1 Lübeck, Germany Lübeck, Germany 2018.11.05 17:32

2018.11.06 09:46

16:14

2 Lübeck, Germany Baltic Sea 1) 2019.03.14 15:41

2019.03.15 12:00

20:18

3 3) Hanko, Finland - 2019.03.16 07:16

- -

4 Gdynia, Poland Baltic Sea 1) 2019.03.17 10:31

2019.03.18 07:19

20:47


2019.04.28 07:39

20:23


2019.06.28 13:13 23:48

7 Hanko, Finland Gdynia, Poland 2019.06.29 05:44

2019.06.30 16:15 34:30

8 On departure from St. Petersburg, Russia 1)

Hanko, Finland 2019.07.09 10:58

2019.07.10 00:31 13:33

9 Lübeck, Germany Baltic sea 1) 2019.08.31 13:56

2019.09.01 14:46 24:49

10 On approach to St. Petersburg, Russia

Gulf of Finland 1) 2019.09.02 11:51

2019.09.03 15:13

27:21

11 On approach to St. Petersburg, Russia

Gulf of Finland 1) 2019.09.30 12:25

2019.10.02 01:25 36:59

1) Detailed GPS locations are available in Appendix C2) Holding time in hours and minutes; from start of ballast to start of deballast; the data were extracted from onsite data logs,

Appendix C3) Deballast of test cycle No. 3 was abandoned due to densities below 100 org/mL for organisms ≥10 and <50 µm


Samples were processed within the shortest possible time. Samples for the enumeration of live organisms ≥50 µm were analysed on board, and, for discharge samples, the total volume of each of the field replicates was analysed, except for test cycle No. 4, where 2.3 m3 (out of 3.3 m3 sample) was analyzed. Discharge samples for the enumeration of live organisms ≥10 and <50 µm were analysed on board within the shortest possible time. The detailed description of the processing of the different samples is provided in Chapter 5). Detailed data for sample temperature logging during storage and transportation are available in Appendix A.

For test cycle Nos. 9 and 10, the samples for organisms ≥50 µm were collected as one replicate by use of one sampling net instead of the usual three replicates. The alternative sampling procedure was in accordance with the ETV Protocol and was not considered to have affected the results. This event is described in Deviation No. 1 to the Test Plan (Appendix B).

Table 1.2 summarizes the inlet water conditions including water quality parameters and organism concentrations during ballast operations.

Table 1.2 Inlet water conditions during ballast operations.

Test cycle

Salinity (PSU)

Temp. (°C)

TSS (mg/L)

POC (mg/L)

DOC (mg/L)

MM (mg/L)

UV-T 1) (%)

Organisms ≥50 µm (org./m3)

Organisms ≥10 and <50 µm (org./mL)

1 17 10 9.8 0.18 3.3 9.7 83 (86) 8,468 22

2 15 5.3 14 0.11 3.5 14 86 (86) 14,821 124

3 2) 6.0 1.5 - - - - - - 79

4 6.9 4.3 13 0.50 3.8 13 72 (75) 10,180 755

5 11 12 7.4 0.82 4.2 6.6 75 (77) 26,357 359

6 10 20 8.1 0.74 4.7 7.4 76 (79) 47,431 956

7 6.5 10 11 0.27 4.3 11 80 (81) 12,762 242

8 1.8 17 2.6 0.48 6.5 2.1 59 (62) 59,458 777

9 12 22 11 0.29 3.7 11 83 (86) 9,882 212

10 0.48 19 4.6 0.29 7.3 4.3 56 (58) 122,783 140

11 0.18 9.9 4.0 0.19 7.0 3.8 55 (57) 39,638 195

1) UV-T measured during the ballast operation; numbers in parentheses indicate value obtained after the sample was filtered through a 0.45-µm syringe filter

2) Deballast of test cycle No. 3 was abandoned, because the density of organisms ≥10 and <50 µm was below 100 org/mL in the inlet water

Test cycle Nos. 1 and 3 were invalid as the average densities of live organisms ≥10 and <50 µm in the inlet water were 22 organisms/mL and 79 organisms/mL, respectively. For test cycle No. 3 the deballast operation was abandoned.


The average densities of live organisms in the inlet water were in accordance with the BWMS Code and the U.S. Coast Guard standards (§162.060-28(e)(2)(ii)) in test cycle Nos. 2 and 4–11:

• The densities of live organisms ≥10 and <50 µm in the inlet water ranged from 124to 956 organisms/mL

• The densities of live organisms ≥50 µm in the inlet water ranged from approx. 10,000to 123,000 organisms/m3

Table 1.3 summarizes the flow rates and UV-T, UV-I and UV dose during ballast operations and the average numbers of live organisms at discharge in water treated by the Hyde GUARDIAN-US BWMS. The live organisms in the ≥10 and <50 µm size class were quantified by microscopy counting after staining with chloromethylfluorescein diacetate (CMFDA) and fluorescein diacetate (FDA).

The Hyde GUARDIAN-US BWMS did not comply with the ballast water discharge standard in test cycles Nos. 5, 6, 8 and 10.

DNV GL concluded that, in test cycle No. 5, the BWMS did not function properly due to holes found in the filter, which resulted in untreated water passing the filter as indicated by high numbers of live organisms in samples taken directly after the BWMS on ballast.

Furthermore, DNV GL concluded that test cycle Nos. 6, 8 and 10 suffered from contamination from the ambient water during deballast, due to improper operation of the BWMS by the ship’s crew and no check valve having been fitted on the overboard discharge line during installation. The findings were a result of an extensive investigation by Hyde Marine into the operational practises and the configuration between the BWMS and the vessel’s automation system. The report produced by Hyde Marine has been attached to this report in Appendix F.

The BWMS functioned according to the performance claim during test cycle No. 1, and, therefore, the trial period was considered started on 5 November 2018.

The deballast operation of test cycle No. 11 was conducted on 2 October, approximately 11 months after the start of the shipboard trial period. Thus, the requirement in the U.S. Coast Guard standards (§162.060-28(b)) that shipboard test cycles must be conducted throughout a period of operation of at least six months was fulfilled.

The Hyde GUARDIAN-US BWMS complied with the ballast water discharge standard in test cycle Nos. 2, 4, 7, 9 and 11.


Table 1.3 Average flow rates and UV-T and UV-I during ballast and deballast operations and average numbers of live organisms in treated discharge water.

Test cycle

Ballast operation Deballast operation

Average flow rate 1) (m3/h)

UV-T 2) (%)

UV-I 3) (mW/cm2)

UV Dose 4) (mJ/cm2)

Average flow rate5) (m3/h)

UV-T 2) (%)

UV-I 3) (mW/cm2)

UV Dose 4) (mJ/cm2)

Organisms ≥50 µm (org./m3)

Organisms ≥10 and <50 µm (org./mL)

Organisms <10 µm V. cholerae

Inlet water

Applied at ballast

Applied at ballast

Inlet water

Applied at deballast

Applied at deballast

1 6) 701 83 (86) 469 214 711 84 (86) 475 213 3.3 1.2 Absent

2 682 86 (86) 445 208 659 86 (87) 459 223 0.91 4.7 Absent

3 6) 7) 684 - 377 177 - - - - - - -

4 611 72 (75) 821 436 660 83 (86) 435 211 7.5 2.0 Absent

5 8) 662 75 (77) 556 269 637 81 (82) 548 272 93 49 Absent

6 9) 644 76 (79) 568 293 527 79 (80) 425 252 69 9.5 Absent

7 632 80 (81) 523 250 508 81 (83) 357 248 9.2 2.5 Absent

8 9) 558 59 (62) 334 193 479 61 (64) 354 244 19 8.2 Absent

9 633 83 (86) 532 268 558 85 (86) 457 259 2.0 0.33 Absent

10 9) 346 56 (58) 250 232 440 59 (61) 279 208 190 0.50 Absent

11 381 55 (57) 233 199 454 57 (58) 259 182 3.0 2.8 Absent

1) Average flow rate after filtration during ballast operation of treated water, measured by the BWMS flow meter located after the filter; extracted from BWMS log 2) UV-T measured during the ballast operation; numbers in parentheses indicate value obtained after the sample was filtered through a 0.45-µm syringe filter 3) UV-I average at stable operating conditions logged during ballast and deballast operation; the data were extracted from the BWMS log 4) UV dose average at stable operating conditions logged during ballast and deballast operation; the data were extracted from the BWMS log 5) Average flow rate during deballast operation of treated water; extracted from BWMS log 6) Invalid test cycles; densities of live organisms ≥10 and <50 µm in the inlet water were below the validity criterion 7) Deballast of test cycle No. 3 was abandoned 8) Test cycle No. 5 was concluded invalid by DNV GL due to a filter failure (details provided in Appendix F) 9) Test cycle Nos. 6, 8 and 10 were concluded invalid by DNV GL due to an installation issue of the BWMS (details provided in Appendix F)

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