m2d1: introduction to cell culture introduction to cell culture • module 1 data summary due monday...
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M2D1: Introduction to cell culture
• Module 1 Data Summary due Monday at 5pm !
★ submit to Stellar• Blog Posts due next week.
• Primer design memos due Thursday at 10pm
• Talk more on Tuesday
• Office Hours:
Noreen([email protected]) Sunday 10-12pm 56-302
and Monday 2-4pm 16-429b
Shannon ([email protected]) Sunday 12-2pm 56-302
Leslie([email protected]) Monday 1-2pm
Email us to meet outside of office hours!!!
Announcements
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Slide from M2D1 Lecture — Prof. Samson!
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Slide from M2D1 Lecture — Prof. Samson!
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Module 2 Overview — Authentic Research!
“Normal cells”“DNA repair-deficient
cells”
“Normal cells +inhibitor of DNA repair”
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Tools to study DNA repair: Our model system
“Normal cells” “DNA repair-deficient cells”
=No Ku80
M2D1 Start M2D2
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Mammalian cell culture — Tissue culture medium
What do cells need to survive?
Food(s):
Non-food(s):
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http://www.sigmaaldrich.com/catalog/product/sigma/cls3815?lang=en®ion=US
Mammalian cell culture — ‘Splitting’ cells
FlaskPlate
1. Rinse with PBS — why?
2. Detach cells — why?
3. Count cells — why?
4. Add to new culture vessel — why?
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Mammalian cell culture — ‘Plating’ cells
http://www.allcells.com/blog/how-to-count-fresh-primary-cells/
https://www.youtube.com/watch?v=pP0xERLUhyc
http://www.cellsignet.com/media/templ.html
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Today in lab:★ Seed cells for Western blot analysis of Ku80 expression:
Blue/Pink/Purple/Platinum— in TC first
★ Leslie R. will be here to talk about abstracts at 2pm
★ Learn about our system:1. Read paper from Jeggo lab
• Answer questions on wiki in your EN notebook• This is a preview of what we’ll be talking about — don’t stress• Speaking of — share your notebook with Nova!