Poster presentations / International Journal of Gynecology & Obstetrics 119S3 (2012) S531–S867 S597

early menopause, sterlity, recurrent abortions and intrauterine fetal

growth retardation. The only treatment for celiac disease is a

gluten free diet and this treatment is also known as ameliorate

gynaecological symptoms.

The main aim of the study was to investigate the association

between coeliac disease and reproduction.

Materials: During the period May 2009 to December 2012,

100 proven coeliac patients in the department of obstretics

and gynaecology of DMC&H, Ludhiana were evaluated for their

gynaecological problems.

Methods: In patients with infertility and recurrent abortions,

gynaecological, endocrine and hematological investigations were

done. All patients are on gluten free diet and were reassessed for

outcome of pregnancy.

Results: A total of 100 proven celiac patients were enrolled.

The mean age at presentation was 29.4years. Thirty patients had

menstrual abnormality. Reproductive outcome in all the patients

were variable, 28 patients were infertile, 21 patients had recurrent

abortions and the rest had a term pregnancy.

Conclusions: An assciation has been observed between celiac

disease and oligomenorrhoea, hypomonorrhoea, dysmenorrhoea

and metrorrhagia. A correlation has emerged for coeliac disease and

threatened abortion, geatational hypertension, placental abruption.

Silent celiac disease can be considered in patients with unexplained

infertility.

M200

MATERNAL OBESITY (MO) INCREASES OXIDATIVE STRESS (OS)

IN MALE OFFSPRING (OFF) TESTES: PREVENTED BY

PRE-PREGNANCY MATERNAL DIETARY INTERVENTION (DINT)

E. Zambrano1, L.A. Reyes1, C.C. Vega1, O. Saldana1, P. Nathanielsz2,

F. Larrea1. 1Reproductive Biology, Instituto Nacional de Nutricion

Salvador Zubiran, Mexico City, Mexico; 2The University of Texas

Health Sciences Center at San Antonio, San Antonio, TX, United States

Objectives: Maternal obesity adversely affects offspring health:

metabolic disorders, and endocrine function. We observed an

increase OS and impaire reproductive function in OFF of MO rats.

We hypothesized that MO affects OFF testes function and this could

be reverse by our model of DINT.

Materials:We studied female Wistar rats fed fromweaning through

pregnancy and lactation on chow (C) or high energy obesogenic diet

(OD). A third group was switched from OD to C at to postnatal day

(PND) 90 and then chow diet (DINT).

All mothers were bred at PND 120 and ate the same diet during

pregnancy until weaning. OFF ate C diet from weaning.

Methods: Fertility rate was tested at PND 440. Six male OFF

(different litters) were euthanized at PND 450 by decapitation.

Fat depots were weighed. Adiposity index (AI): fat tissue weight

(thoracic and visceral)//weight body ×100. Testis was dissected

and homogenized in saline solution 0.9%. OS test were performed

(MAD) in serum and testis by spectrophotometry. GPx was analyzed

by chemical colorimetry. One way ANOVA; p < 0.05.

Fig 1. OFF of C (open), OD (solid) and DINT (gray) rats. Data

Mean±SEM, p < 0.05 for data not sharing a letter.

Results: At PND 450 OFF weight was C: 547±25a, OD: 627±13b

and DINT: 563±9a (g), gonadal fat C: 12±0.4a, OD: 16.5±1.3b,

DINT: 14±0.3ab (g), and AI, C: 6.0±0.3a, OD: 8.2±0.5b and DINT

6.0±0.2a. OD OFF fertility rate (FR) were decreased, DINT restored

FR completely to normal (Fig. 1A). Malondialdehyde (MDA) serum

level was significantly increased in OD and recuperated in DINT

OFF (Fig. 1B). In DINT OFF testis, MDA and Glutathione peroxidase

(GP) activity were partially recuperated (Fig. 1C–D).

Conclusions: MO decreased OFF fertility rate associated with

increased testes OS markers and antioxidant enzymes. DINT

recuperated functional FR and reversed partially oxidative damage.

M201

UTERINE FIBROIDS, MANAGEMENT AND EFFECT ON

REPRODUCTION

T.C. Malayan1. 1Samuel Merritt University, San Francisco, CA, United

States

Objectives: At least one in four women develops uterine fibroids in

her lifetime (King & Overton, 2011). There are many treatment

options for fibroids, but no defined gold standard. Recently,

techniques such as laparotomy, hysteroscopy and myomectomy

have provided additional options. Fibroids are thought to be

responsible for 2–3% of cases with infertility (Davis, 2010). Does

removal of the cause cure the symptom?

Methods: One comparative study investigated the chances of

pregnancy in women who underwent laparoscopic myomectomy.

In this study, 212 women complaining of infertility and recurrent

miscarriages were recruited. 106 women underwent myomectomy

and the other 106 did not receive treatment.

Results: A higher delivery rate, 42% vs. 11%, was observed in the

surgical group (Abbiati, 2008).

Conclusions: Studies regarding fibroids and treatment are few and

inconsistent. Although some evidence suggests that myomectomies

may improve fertility rates and decrease pregnancy complications,

determining guidelines for the management of fibroids requires

further research. The most effective treatment for fibroids in

women attempting to become pregnant should be decided using

a personalized approach. Her age, the number, dimension, and

location of the fibroids, the symptoms and the expertise of the

surgeon should all be considered when deciding on the best

treatment option. Women need to be informed of the pros and

cons of each option, risks of fibroids during pregnancy, and the

risks of treatment. The goal should be to come up with a shared

decision between doctor and the patient.

M202

Withdrawn