lung cancer mortality and diagnostic methods
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Analysis of exhaled breath with chromatography or electronic nose . D iagnosis of lung cancer by multifactorial logistic regression analysis. - PowerPoint PPT PresentationTRANSCRIPT
Analysis of exhaled breath with chromatography or electronic nose. Diagnosis of lung cancer by
multifactorial logistic regression analysisDr.med. Māris Bukovskis 1 2 4, Dr.biol. Gunta Strazda 1 2 3, Dr.med Uldis
Kopeika 2 5, Dr.biol.Normunds Jurka 2, Līga Balode 2, Dr. Jevgenija Aprinceva 3, Dr. Ainis Pirtnieks 5, Prof. Immanuels Taivans 2 3
1 Department of Pulmonology and Allergology, Pauls Stradins Clinical University Hospital, 2 Institute of Experimental and Clinical Medicine, University of Latvia, 3 Faculty of Medicine, University of Latvia, 4 Department of Internal Medicine, Faculty of Medicine, University of Latvia, 5 Department of Thoracic Surgery, Pauls Stradins Clinical University Hospital
Lung cancer mortality and diagnostic methods• Lung cancer is the leading cancer killer in both men
and women and causes more deaths than the next three most common cancers combined (colon, breast and prostate)
• 58 - 73% of stage I lung cancer patients suvive 5-years compared to 9 - 24% of patients with stage III lung cancer
• Available diagnostic methods - nonsensitive (sputum, chest x-ray), expensive (CT) or invasive (FB)
Goldstraw P et al. J Thorac Oncol 2007American Cancer Society Cancer. Facts and Figures 2012
VOC’s in exhaled breath
Lung cancer sniffer dogsCBC News Aug 17, 2011
Gordon SM et al. Clin Chem 1985Machado et al. AJRCCM 2005
Chen X et al. Cancer 2007
Is this so easy?
Complex consisting of desorbtion unit, gas chromatograph and mass spectrometer
Chromatogram showing multiple peaks representing particular chemical substances
Time (s)
Rel
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ectri
c re
sist
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Baseline
Response of 32 sensors to air sample
Functional principles of electronic nose
ΔRmax
Cyranose 320
Methods
• 475 patients in total, 252 of them lung cancer patients, and 223 patients with different lung diseases and healthy volunteers, were recruited in the study
• Collection and sampling of exhaled air by electronic nose (Cyranose 320)
• Analysis of “smell prints” by multifactorial regression analysis
ResultsClassification of cases in nonsmokers
Lung cancer No cancer
Lung cancer 128 12 PPV 91.4%
No cancer 5 120 NPV 96.0%
Sensitivity 96.2% Specificity 90.6%
Classification of cases in smokers
Lung cancer No cancer
Lung cancer 114 7 PPV 94.2%
No cancer 5 84 NPV 94.4%
Sensitivity 95.8% Specificity 92.3%
Why is this so valuable?
• Advantages of exhaled breath analysis by electronic nose: - cheap - easy to do - non-invasive - sufficiently accurate - possible to do in a large number of patients at risk (all
smokers > 50 years of age)
• Chromatography analysis is valuable for scientific research purposes
Thank You for Your Attention!
How to sniff out the disease?