long-term potentiation as a physiological phenomenon
DESCRIPTION
Long-term Potentiation as a Physiological Phenomenon. From Mechanisms of Memory by J. David Sweatt, Ph.D. The Entorhinal/Hippocampal System. Entorhinal Cortex. Perforant Pathway. Dentate Gyrus. Stratum Lacunosom Molecular inputs. Mossy Fiber. Lateral Septum, Contralateral CA1. - PowerPoint PPT PresentationTRANSCRIPT
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Long-term Potentiation as a Physiological Phenomenon
From Mechanisms of Memory by J. David Sweatt, Ph.D.
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Lateral Septum,
Contralateral CA1
EntorhinalCortex
Dentate Gyrus
CA3
IpsilateralCA1
PerforantPathway
Mossy Fiber
EntorhinalCortex
Subiculum Lateral Septum
Amygdala,Cortex
SchafferCollaterals
Norepinephrine,
Acetylcholine,
Serotonin
GABAergic Interneuron
CA1 Axon
Schaffer Collaterals
The Entorhinal/Hippocampal System
Str
atu
m L
acu
no
som
Mo
lecu
lar
inp
uts
RecurrentConnections
SLM Inputs
Dopamine,
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Bliss and Lomo’s First Published LTP Experiment
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StimulatingElectrode
RecordingElectrode
Electrodes in a Living Hippocampal Slice
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Stimulating Schaffer Collaterals
in Area CA3
Recording in Stratum Pyramidale
in Area CA1
Recording in Stratum Radiatum
in Area CA1
Stimulus Artifact
Fiber VolleyEPSP
Recording Configuration and Typical Responses in a Hippocampal Slice Recording Experiment
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-20 0 20 40 60 80 1000
1
2
3
Time (min)
No
rmal
ized
Initi
al S
lope
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1
2
3
Time (min)
No
rmal
ized
Initi
al S
lopeB
A Input/Output
0 10 20 30 400
250
500
750
Stimulus Intensity ( A)
Slo
pe
fEP
SP
( V
/ms)
Fiber Volley
0 10 20 30 400.0
0.1
0.2
0.3
0.4
Stimulus Intensity ( A)
Fib
er V
olle
y A
mp
litu
de
(V
)
Input/Output vs Fiber Volley
0.0 0.1 0.2 0.3 0.40.00
0.25
0.50
0.75
Fiber Volley Amplitude ( V)
Slo
pe
fEP
SP
( V
/ms)
An Input/Output Curve and a Typical LTP Experiment
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Figure 6: APV Block of LTP
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75
100
125
150
175
200
225Vehicle50 M APV
APV
Time(min)
fEP
SP
slo
pe
(% o
f b
asel
ine)
APV Block of LTP
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…
100-Hz 100-Hz 100-Hz 100-Hz
200
msec200
msec
200
msec
10 msec between pulses
• 5-Hz burst frequency• 10 bursts per train• 3 trains, 20-sec intertrain interval
A
B
Time(min)
fEP
SP
slo
pe
(% o
f b
asel
ine)
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75
100
125
150
175
200
LTP Triggered by Theta Burst Stimulation
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Pairing LTP
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Gly
Glu++++
----
---
+++
SynapticGlutamate Alone
CytoplasmSynaptic Cleft
Mg++
Ca++
Glu
Ca++
++++
----
Mg++
Gly
---
+++
Glutamate plusMembrane
Depolarization
CytoplasmSynaptic Cleft
Ca++
Coincidence Detection by the NMDA Receptor
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Back Propagating Action Potentials
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Timing of Back-propagating Action Potentials with Synaptic Activity
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EP
SP
’s
SynapticActivity
NEChange in
Local excitability1
2
Synapse
LTP?
A B
The Dendritic Tree and Regulation of Action Potential Propagation
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NMDAR independent LTPA
B C
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75
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200HzAP-5
Time (min)
% S
lop
e p
EP
SP
(Sta
nd
ard
ized
to
Bas
elin
e)
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-90 -60 -30 0 30 60 90 120 150 18080
100
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Seconds%
bas
elin
e fE
PS
P
PTPPPF