2

3

List of projects handled and of publications is given on page 7 and 8

List of seminars/training courses conducted is given on page 9

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List of available major equipment is given on page 7

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6

Research and Extension Projects of ABFS.

No. Project Title Duration Funding by

Budget (in Rs. Lakhs)

1 Crop Improvement through Biotechnologies 2009-10 RKVY 334.002 Establishment of Bio Control Lab at ABF 2009-10 SHM 27.00 3 Popularization of Softer Biotechnologies for Agriculture Improvement 2011-16 DBT 67.59 4 Crop Improvement through Biotechnologies 2011-12 RKVY 200.005 Screening of BLB and Blast resistant rice lines 2012-13 Makteshim

Agan India05.00

6 FTTF-Crop Productivity Improvement Through Seed Village Scheme 2012-15 NABARD 46.44 7 Identification and utilization of new sources of BPH resistance through

MAS in rice improvement 2013-14 RKVY 39.00

8 Genetic diversity of Abiotic stress tolerant Entomopathogenic fungai in Plant Production

2013-14 RKVY 13.00

9 Development and standardization of Abiotic stress tolerant Cyst based liquid formulations of Azotobacter and Azospirillum

2013-14 RKVY 16.00

10 Development of Groundnut transgenic plant carrying Eukaryotic translation initiations factor 4A under stress inducible promotor for improved Abiotic stress tolerance

2013-14 RKVY 19.00

11 Development of Herbicide Tolerant Rice and Mungbean through genetic engineering

2013-14 RKVY 11.00

12 Documentation of Indigenous coping strategies to climate change in different areas of agriculture with special reference to drought prone areas of Andhra Pradesh and Karnataka

2013-16 ICAR-NICRA

35.00

13 Socio-Economic upliftment of rural and Peri Urban SC/ST Population of Srikakulam District through Agri Biotechnologies

2013-16 DBT,GOI 34.92

14 Endophyte mediated enhancement of plant performance in relation to biocontrol and gene expression under drought stress

2013-16 DBT,GOI BIOCARE

14.80

15 Molecular marker-assisted introgression of two major blast resistance genes and a major QTL for grain yield under drought stress in rice

2013-17 DBT, GOI 16.68

16 Differential expression of RNAs in maize under drought stress inoculated with ACC deaminase producing rhizobacteria

2014-17 DST-GOI 19.00

17 Seed Distribution under TSP 2014-15 IIMR 12.0018 Seed Distribution under TSP 2014-16 IIOR 21.7519 Establishment of Biofertilizers production unit at Ananatapuram 2015-16 RKVY,AP 115.0020 Establishment of IBRC at Ananatapuram 2015-16 RKVY 122.0021 Short term training course on “Molecular Cloning and transgenic

technologies for crop improvement”Sept..2015

DBT, GOI 03.00

22 Molecular cross-talk between defense pathways in rice: From antagonism to synergism

2015-18 ICAR-NASF 63.34

23 Development of Transgenic Cotton 2015-16 RKVY 15.0024 Identification and Utilization of new sources of BPH resistance through

MAB in Rice2015-16 RKVY 12.00

25 Field Evaluation of liquid bioformulations of nitrogen fixing and phosphate solubilizing bacterial

2015-16 RKVY 14.00

26 Micropropagation of Strawberry 2016-17 M/S Ichigo 03.00 27 Micropropagation of Blue berry 2016-17 M/S Simply

Fresh04.50

28 Micro propagation of Pomegranate 2016-17 M/S PYR 00.35 29 Molecular basis of differential resistance in rice line RP2068-18-3-5

against BPH and WBPH2017-19 DST-GOI

NPDF19.00

30 Study of genetic diversity and population structure of Brown Plant Hopper (BPH), Nilaparvata lugens (Stal.) from NE India

2017-20 DBT, GOI 15.60

31 Public Perception of Genetically Modified Animals, Science, Untility and Society (PEGASUS)

2009-12 European Commission

23.62

32 Agri Clinics and Agri–Business Center (AC-ABC) 2012-13 MANAGE 9.31

Major Equipments available at ABFEquipment Equipment/greenhouse

Real-Time PCR HPLCNano spectrophotometer Fluorescent MicroscopeMultiporator Millipore water purification unitHybridization oven Autoclaves (Horrizontal & Vertical)Ligation bathDeep Freezer (-80oC & - 20oC) Bench top refregirated CentrifugeAgarose Electrophoresis unit Refrigerated CentrifugeRefrigerated Shaking Incubator Shade net houseGradient PCR PolyhouseSDS PAGE Unit Insect rearing greenhousesGel Doc Unit Transgenic greenhouse

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List of Publications 1. Chandra Teja KNP and Rahman SJ (2017). Effect of media pH on the growth of Entomopathogenic fungi isolated from

different rhizosphere soils. International Journal of Bioassays, 6(3): 5325-5327. Doi: 10. 21746/ijbio.2017.03.0092. Chandra Teja KNP and Rahman SJ (2017). Evaluation of the growth and sporulation of different entomopathogenic fungi

in different liquid and solid media at varied concentrations. International Journal of Bioassays, 6(8): 5459-5464. Doi: 10. 21746/ijbio.2017.08.003

3. Ojha A, Sinha DK, Padmakumari AP, Bentur JS and Nair S (2107). Bacterial Community Structure in the Asian Rice Gall Midge Reveals a Varied Microbiome Rich in Proteobacteria. Scientific Reports, 7: 9424. Doi: 10.1038/s41598-017-09791-0 (IF: 4.8)

4. Rao TSRB, Juturu VN, Reddy PS, Reddy MK and Mallikarjuna G (2017). Expression of Pennisetum glaucum eukaryotic translational initiation factor 4A (PgeIF4A) confers improved drought, salinity, and oxidative stress tolerance in groundnut. Frontiers in Plant Sciencs, 8:453. Doi: 10.3389/ fpls.2017.00453 (IF: 4.2)

5. Sandhya V, Ali Sk Z, and Prasad SSKV (2017). Plant growth promoting endophytes and their interaction with plants to alleviate abiotic stress. Current Biotechnology, 6:1-12. Doi: 10.2174/2211550106666161226154619

6. Sandhya V, Shrivastava M, Ali Sk Z, Prasad SSKV and Shrivastava M (2017). Endophytes from maize with plant growth promotion and biocontrol activity under drought stress. Russian Agricultural Sciences, 43:22-24.

7. Sinha DK, Atray I, Agarrwal A, Bentur JS and Nair S (2017). Genomics of the Asian rice gall midge and its interactions with rice. Current Opinion in Insect Science, 19:76-81. Doi.org/10.1016/j.cois.2017.03.004 (IF: 3.6)

8. Bentur JS, Rawat N, Divya D, Sinha DK, Agarrwal R, Atray I and Nair S (2016). Rice-gall midge interactions: Battle for survival. Journal of Insect Physiology, 84:40-49. Doi.org/ 10.1016/ j.jinsphys. 2015.09.008. (IF: 2.5)

9. Chakraborthy M, Sairam Reddy P, Laxmi Narasu VM, Gaurav Krishna and Rana D (2016). Agrobacterium-mediated genetic transformation of commercially elite rice restorer line using nptII gene as a plant selection marker. Physiology and Molecular Biology of Plants, 22(1): 51-60. Doi: 10.1007/s12298-015-0334-y. (IF: 0.8)

10. Chakraborthy M, Sairam Reddy P, Mustafa G, Rajesh G, Laxmi Narasu VM, Udayasuriyan V and Rana D (2016). Transgenic rice expressing the cry2AX1 gene confers resistance to multiple lepidopteran pests. Transgenic Research, 25: 665-678. Doi; 10.1007/s11248-016-9954-4. (IF: 2.3)

11. Chandra Teja KNP and Rahman SJ (2016). Characterisation and evaluation of Metarhizium anisopliae (Metsch.) Sorokin strains for their temperature tolerance. Mycology, 7: 171-179. Doi: 10.1080/21501203.2016.124711.

12. Divya D, Singh YT, Nair S and Bentur JS (2016). Analysis of SSH library of rice variety Aganni reveals candidate gall midge resistance genes. Functional & Integrative Genomics. 16:153-169. Doi: 10.1007/s10142-016-0474-3. (IF: 3.4)

13. Gopalakrishna M, Juturu VN, Mallikarjuna G, Kirti PB and Yadav SK (2016). Optimization of Agrobacterium-mediated genetic trans-formation of shoot tip explants of green gram (Vigna radiata (L.) Wilczek). Plant Cell Tissue and Organ Culture, 127:651-663. Doi. 10.1007/s11240-016-1085-3. (IF: 2.0)

14. Juturu VN, Gopalakrishna M, Akila CS, Puli COR and Mallikarjuna G (2016). High frequency induction of multiple shoots and plant regeneration from zygotic embryo axis explants of cotton cultivar L-604 (Gossypium hirsutumL.). International Journal of Bioassays, 5(9): 4791-4796. Doi: 10.21746/ijbio.2016.09.002.

15. Juturu VN, Gopalakrishna M, Mallikarjuna G, Puli COR and Akila CS (2016). Optimization of in vitro regeneration protocol for a popular indica rice (Oryza sativa L. cv. Swarna). Annals of Plant Sciences, 5(8):1395-1401. Doi: 10.21746/ aps.2016.08.002

16. Mallikarjuna G, Rao TSRB and Kirti PB (2016). Genetic engineering for peanut improvement: current status and prospects. Plant Cell Tissue and Organ Culture, 125(3):399-416. Doi: 10.1007/s11240-016-0966-9. (IF: 2.0)

17. Rakesh Kumar, Vinai Kumar, Sreenu Kadiri and Sairam Reddy Palicherla (2016). Epidemiology and Diagnosis of Chilli leaf curl virus in Central India, a major chilli growing region. Indian Phytopathalogy, 69(4S): 61-64.

18. Prasad SSKV, Sandhya V, Shrivastava M, Ali Sk.Z. (2016). Multifunctional Pseudomonas putidastrain FBKV 2 from arid rhizosphere soil and its growth promotional effects on maize under drought stress. Rhizosphere, 1: 4-13. Doi: 10.1016/j.micres.2015.12.003.

19. Prasad SSKV, Sandhya V, Manjari Shrivastava M, and Ali Sk.Z (2016). Enhancement of drought stress tolerance in crops by plant growth promoting rhizobacteria. Microbiological Research, 184:13-24. Doi: 10.1016/j.micres.2015.12.003 (IF: 3.1)

20. Bhattacharyya D, Mallikarjuna G,and Lee YH (2015). Volatile Indole produced by Rhizobacterium Proteus vulgaris JBLS202 Stimulates growth of Arabidopsis thaliana through auxin, cytokinin, and brassinosteroid pathways. Journal Plant Growth Regulation, 34(1): 158-168. Doi: 10.1007/s00344-014-9453-x. (IF: 2.0)

21. Prasannakumar KL, Sujatha B, Subbareddy B and Pakki Reddy G (2015). Technological inovations management in micropropogation industry. International Journal of Entrepreneurship & Business Environment Perspectives, 4(3):1886-1895.

22. Sandhya V and Ali Sk.Z. (2015) The production of exopolysaccharide by Pseudomonas putida GAP-P45 under various abiotic stress conditions and its role in soil aggregation. Microbiology, 84:512-519. Doi: 10.1134/S0026261715040153. (IF: 0.8)

23. Srichandana B, Durgarani ChV, Gouthami T, Yamini KN, Arun PKN, Jamaloddin Md and Swathi G (2015). Screening of rice genotypes for resistance to brown planthopper biotype 4 and detection of BPH resistance genes. International Journal of Sciences Biotechnology and Pharma Research, 4(2).

24. Juturu VN, Gopala Krishna Ma, Surabhi GK, Kirti PB (2015). Current status of tissue culture and genetic transformation research in cotton. Plant Cell Tissue and Organ Culture, 120(3):813-839.Doi: 10.1007/s11240-014-0640-z. (IF: 2.0)

25. Ali Sk Z, Sandhya V and Rao LV (2014). Isolation and characterization of drought tolerant ACC deaminase and exopolysaccharide producing fluorescent Pseudomonas sp. Annals in Microbiology, 64:493-502.

26. Ramesh K, Padmavathi G, Deen R, Pandey MK, Lakshmi VJ and Bentur JS (2014). Whitebacked planthopper Sogatella

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furcifera (Horváth) (Homoptera: Delphacidae) resistance in rice variety Sinna Sivappu. Euphytica, 200(1):139-148. Doi: 10.1007/s10681-014-1175-4. (IF: 1.6)

27. Prasannakumar KL and Sujatha B (2014) Innovations in human resource management to scaleup cottage industry. Internatinal Journal of Social Science and Interdisciplinary Research, 3(8):40-48.

28. Sama VSAK, Rawat N, Sundaram RM, Himabindu K, Naik BS, Viraktamath BC and Bentur JS (2014). A putative candidate for the recessive gall midge resistance gene gm3 in rice identified and validated. Theoretical Applied Genetics, 127(1): 113-124. Doi: 10.1007/s00122-013-2205-7. (IF: 4.1)

29. Sandhya Vardharajula and Ali Sk Z (2014). Exopolysaccharide Production By Drought Tolerant Bacillus Spp. And Effect On Soil Aggregation Under Drought Stress. J Microbiol Biotechnol Food Science, 4(1): 51-57. Doi: 10.15414/jmbfs.2014.4.1.51-57

30. Scott B, Pakki Reddy G, and Millar K (2013). Bioscience and Innovation Research: Examining the GM animals case with Indian researchers using the ethical matrix. Asian Biotechnology and Development Review. 15(2): 1-17.

For pdf reprint of any of the publications contact concerned Faculty

List of book chapters:1. Prasad SSKV, Sandhya V and Ali SkZ. (2016). Plant-rhizobacteria interactions mitigates drought

stress. Scientific Publishers (INDIA), ISBN: 978-81-7233-990-62. Sandhya V and Ali SkZ (2016). Endophytic bacteria as plant growth promoters. Scientific Publishers (INDIA),

ISBN: 978-81-7233-990-63. Ali Sk Z, Reddy GP and Sandhya V (2015) Organic Farming: Food security of small holding farmers. In: Leire

E San-Epifanio and M De RenobalesScheifler (Edt) Envisioning a future without food waste and food poverty: Societal challenges. Wageningen Academic Publishers, Netherland, pp. 309- 316.

4. Sandhya V, Reddy GP, Ali SkZ and Kumar PKL (2015) Traditional Knowledge and sustainable agriculture: The strategy to cope with climate change. In: Leire E San-Epifanio and M De Renobales Scheifler (Edt) Envisioning a future without food waste and food poverty: Societal challenges. Wageningen Academic Publishers, Netherland pp.109-118.

5. Makkar GS and Bentur JS (2017) Breeding for stem borer and gall midge resistance in rice. Pp. 323-352 In: Arora R and Sandhu S (Edt) Breeding Insect Resistant Crops for Sustainable Agriculture. Springer International Publishing.

Skill Development Training Programmes Organized by Agri Biotech Foundation

S. No. Training Name, Dates (No. of Participants) S. No. Training Name, Dates

(No. of Participants)

1Training Programme on “Biopesticides and Biofertilizers – Production and Usage” 17-31 March, 2010 (12)

16 Summer Internship In Biotechnology for Undergraduates, 1st -31st May, 2016 (22)

2 Three weeks hands on training programme in Plant Molecular Biology 19th April to 10th May, 2010 (22)

17 Hands-on Training Programme in Plant Tissue Culture and Applications, 20-29th June, 2016 (20)

3Three months Finishing School in “Agricultural Biotechnology” 20th December, 2010 to 21st March, 2011 (15)

18 Molecular tools for gene identification and expression studies in crop plants, 19-24th September, 2016 (19)

4 One day training programme for trainees from JNTU. 6th July, 2011 (40)

19 Hands-on Training Programme in Plant Tissue Culture, 17-22 October, 2016 (13)

5 Two day Hands on Training Programme on “Plant Molecular Biology-Tools and Techniques”, 29 – 30th November, 2011 (16)

20 Hands on skills on Real Time PCR and Data Analysis, 4-6th January, 2017 (10)

6 “Techniques in plant tissue culture and Biotechnology”, 20th April, 2012 (60)

21 Techniques in Plant Molecular Biology, 13 – 18th March, 2017 (10)

7Two months training programme on “skill development in Agri-Biotechnologies” for Agriculture polytechnic students, 23rd July – 22nd Septmeber, 2012 (30)

22 Summer Internship in Biotechnology for Undergraduates, 1st -31st May, 2017 (20)

8 Three week Refresher course on “Plant Biotechnology” for in-service candidates, 1st – 21st October, 2012 (15)

23 Hands on training on “Introduction to Molecular Entomology”, 7-12th August, 2017 (12)

9 “Agri Clinics and Agri Business Centers Scheme”, 21st January – 21st March, 2017 (25)

24 Hands on skills on Real Time PCR and Data Analysis, 1st -2nd June, 2017 (20)

10 “Refresher Course on Plant Molecular Biology” 16-25th July, 2013 (20)

25 Recent Techniques in molecular Microbiology,20-25th June, 2016 (30)

11 Summer Internship In Biotechnology for Undergraduates 1st – 31st May 2014 (14)

26 Recent Techniques in molecular Microbiology,29th August-31st 2016 (22)

12A ten day refresher course on “Molecular cloning and transgenic technologies for crop improvement” 9 – 18th March, 2015 (12)

27 Recent Techniques in molecular Microbiology, 30th May-4th June, 2016 (38)

13 Summer Internship In Biotechnology for Undergraduates, 1st – 31st May 2015 (13)

28 Recent Techniques in molecular Microbiology,1st to 3rd September, 2016 (44)

14Short term training course on “Molecular cloning and transgenic technologies for crop improvement” 1-16th September, 2016 (21)

29 Recent Techniques in Microbiology Biotechnology, 5th and 6th September, 2017 (80)

15Training course on “Basics in Plant molecular biology techniques” for B.Sc students from RBVR Reddy Women’s college, Hyderabad 16-19 August 2017 (34)

30 Techniques in Molecular Biology 9 -14 October, 2017 (20)

31 Trainings programmes for, Community Resource Persons, Agri Entrepreneurs and FarmersHands on skills on Organic Agriculture Total no. =37; Total participants= 2004

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