lightsight ™ 2.0 software © 2008 applera corporation and mds inc
TRANSCRIPT
LightSight™ 2.0 Software© 2008 Applera Corporation and MDS Inc.
LightSight™ 2.0 Software© 2008 Applera Corporation and MDS Inc.
Make light work of metabolite IDMake light work of metabolite IDThe result—faster, more comprehensive metabolite identification.The result—faster, more comprehensive metabolite identification.Now the metabolite identification workflow is even easier and more efficient with automated method development.Now the metabolite identification workflow is even easier and more efficient with automated method development.Designed by customers with input from pharmaceutical companies worldwide.Designed by customers with input from pharmaceutical companies worldwide.Specifically designed workflow for the Met ID labSpecifically designed workflow for the Met ID lab
• Automated method builder creates a full set of optimal IDA methods on the QTRAP® System
• Automated method builder creates a full set of optimal IDA methods on the QTRAP® System
• Auto-built expert-level methods provide increased metabolite detection within a single IDA analysis
• Auto-built expert-level methods provide increased metabolite detection within a single IDA analysis
• Auto-built “pMRM” methods increase metabolite detection significantly over traditional strategies
• Auto-built “pMRM” methods increase metabolite detection significantly over traditional strategies
• Intelligent application and automation reduce the need for extensive QTRAP® System operator training
• Intelligent application and automation reduce the need for extensive QTRAP® System operator training
AB SCIEX Drug Discovery Solutions for Metabolite Identification
LightSight™ 2.0 Software© 2008 Applera Corporation and MDS Inc.
AB SCIEX Drug Discovery Solutions for Metabolite Identification
Comprehensive set of features makes light work of metabolite IDComprehensive set of features makes light work of metabolite ID
AcquireMRM
AcquireMRM
AcquirePrecursor
AcquirePrecursor
AcquireNeutral
Loss
AcquireNeutral
Loss
AcquireEMS
AcquireEMS
LightSight™Software
MetaboliteSearch
LightSight™Software
MetaboliteSearch
ACD/MS Processor
ACD/MS Processor
ReportReport
LightSight™ 2.0 Software© 2008 Applera Corporation and MDS Inc.
Make light work of metabolite IDMake light work of metabolite ID
AB SCIEX Drug Discovery Solutions for Metabolite Identification
LightSight™ 2.0 Software© 2008 Applera Corporation and MDS Inc.
LightSight™ 2.0 Software automates Predictive MRM (pMRM)LightSight™ 2.0 Software automates Predictive MRM (pMRM)MRM is the next generation of theoretically predicted metabolite MRMs for Information Dependent Acquisition (IDA)MRM is the next generation of theoretically predicted metabolite MRMs for Information Dependent Acquisition (IDA)Also predicts MRM transitions for metabolites that are a result of combined transitionsAlso predicts MRM transitions for metabolites that are a result of combined transitions
Biotransformation List
Biotransformation List
Predicted FragmentsPredicted
Fragments
+Automated
Method Built With pMRMs
Automated Method Built With pMRMs
IDA collects MS/MS data to confirm
metabolites
IDA collects MS/MS data to confirm
metabolites
AB SCIEX Drug Discovery Solutions for Metabolite Identification
LightSight™ 2.0 Software© 2008 Applera Corporation and MDS Inc.
GSH conjugated metabolites indicates potential reactive metabolitesGSH conjugated metabolites indicates potential reactive metabolitesLightSight™ Software Automates Glutathione Detection to Make light work of metabolite IDLightSight™ Software Automates Glutathione Detection to Make light work of metabolite IDReactive/electrophilic metabolites conjugate with the tri-peptide GlutathioneReactive/electrophilic metabolites conjugate with the tri-peptide GlutathioneOnly the QTRAP® System allows selective HT GSH screening with confirmationOnly the QTRAP® System allows selective HT GSH screening with confirmation
NH
NH
O
O
O
O
OH
NH2
SH
OH
Gly
CysGlu
Three Automated Methods for finding Glutathione usingLightSight™ Software:
› Negative Precursor 272
› Positive Neutral Loss 129
› pMRM with the GSH only biotransformation set
Three Automated Methods for finding Glutathione usingLightSight™ Software:
› Negative Precursor 272
› Positive Neutral Loss 129
› pMRM with the GSH only biotransformation set
Metabolite Profiling is efficient when
Combining LightSight 2.0 Software
And the powerful QTRAP® System
Metabolite Profiling is efficient when
Combining LightSight 2.0 Software
And the powerful QTRAP® System
AB SCIEX Drug Discovery Solutions for Metabolite Identification