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Ligase: joins (seals) the ends of DNA. Vectors: DNA molecule that can replicate & is used to transport foreign DNA. - PowerPoint PPT Presentation

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Vectors: DNA molecule that can replicate & is used to transport foreign DNA

Four types of Vectors: Plasmids; Bacteriophage & other viruses; artificial chromosomes (YAC or yeast artificial chromosome/ DNA + Ori +centromere for sister chromatid separation + telomeres to mark end of chromosome); & Cosmids (plasmid + cos site & can be pckged into phage capsid)

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Transgenic Plant: Vector = Agrobacterium tumefaciens plasmid called Ti (causes crown gall tumors). Plasmid codes for virulence gene and regulates pant growth (codes for plant growth hormones, auxin & cytokinin). It acts like a transposon.

Ti sequence 23 bp direct repeats, that flank the virulence & hormone genes

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Osaka University Knowledge Archive : OUKA...3). Finally, EXO1 exonuclease removes the error-contianing strand, DNA polymerases resynthesize the correct strand, and a DNA ligase seals

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DNA Replication and Repair – Sep. 26-28, 2007 Dr. Jim ...education.med.nyu.edu/courses/molecular/molbio/repnotes.pdf · fills the gap, DNA ligase joins the two strands. In each

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Biologia Genômica 2º Semestre, 2017 Replicação de DNA em ... · Okazaki fragment to the 5′ beginning of the next fragment. FIGURE 25: DNA ligase seals nicks between adjacent

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